Restriction map for
pFN21AB0408
(Circular) (Six-base) MAPSORT of: pFN21AB0408.seq Check: 7636 from: 1 to: 7114
pFN21AB0408 7114 bp
With 182 enzymes: SgfI *
October 20, 2009 10:58 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2209 4294 2209
Size: 2085 5029
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4924 278
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3882 4457 1937
Size: 1945 575 4594
Fragments arranged by size:
4594 1945 575
Acc65I G'GTAC_C
Cuts at: 4435 4435
Size: 7114
AclI AA'CG_TT
Cuts at: 1563 5241 5614 1563
Size: 3678 373 3063
Fragments arranged by size:
3678 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3101 3407 3419 3491 4109 5177 6561 6829
Size: 1469 306 12 72 618 1068 1384 268
Cuts at: 6829 1632
Size: 1917
Fragments arranged by size:
1917 1469 1384 1068 618 306 268 72
12
AfeI AGC'GCT
Cuts at: 3689 3689
Size: 7114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6917
AflIII A'CryG_T
Cuts at: 1203 3510 6013 6990 1203
Size: 2307 2503 977 1327
Fragments arranged by size:
2503 2307 1327 977
AgeI A'CCGG_T
Cuts at: 5945 5945
Size: 7114
AhdI GACnn_n'nnGTC
Cuts at: 5843 5843
Size: 7114
AleI CACnn'nnGTG
Cuts at: 1166 2087 3579 3763 4043 1166
Size: 921 1492 184 280 4237
Fragments arranged by size:
4237 1492 921 280 184
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7114
AlwNI CAG_nnn'CTG
Cuts at: 2224 2633 4234 4309 4391 4857 6429 2224
Size: 409 1601 75 82 466 1572 2909
Fragments arranged by size:
2909 1601 1572 466 409 82 75
ApaI G_GGCC'C
Cuts at: 3423 3423
Size: 7114
ApaLI G'TGCA_C
Cuts at: 2183 3236 3911 5171 6327 2183
Size: 1053 675 1260 1156 2970
Fragments arranged by size:
2970 1260 1156 1053 675
ApoI r'AATT_y
Cuts at: 1493 4423 4649 4801 6910 1493
Size: 2930 226 152 2109 1697
Fragments arranged by size:
2930 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5668 160
Size: 872 4636 1606
Fragments arranged by size:
4636 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 2571 2792 2830 2856 3157 3208 3947
Size: 73 628 221 38 26 301 51 739
Cuts at: 3947 4439 1870
Size: 492 4545
Fragments arranged by size:
4545 739 628 492 301 221 73 51
38 26
BamHI G'GATC_C
Cuts at: 3486 4444 4811 3486
Size: 958 367 5789
Fragments arranged by size:
5789 958 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2873 3477 4045 4435 5890
Size: 325 217 627 1086 604 568 390 1455
Cuts at: 5890 618
Size: 1842
Fragments arranged by size:
1842 1455 1086 627 604 568 390 325
217
BanII G_rGCy'C
Cuts at: 1391 2261 2595 2657 2682 2844 3423 3948 4433
Size: 870 334 62 25 162 579 525 485
Cuts at: 4433 1391
Size: 4072
Fragments arranged by size:
4072 870 579 525 485 334 162 62
25
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3071 3911 4137 4321 928
Size: 971 1172 840 226 184 3721
Fragments arranged by size:
3721 1172 971 840 226 184
BbvCI CC'TCA_GC
Cuts at: 2634 2748 4350 2634
Size: 114 1602 5398
Fragments arranged by size:
5398 1602 114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3685 3719 3769 3803 5305 5339 1903
Size: 34 1748 34 50 34 1502 34 3678
Fragments arranged by size:
3678 1748 1502 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5006 6216 5006
Size: 1210 5904
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7114
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 5725 136
Size: 107 122 71 631 605 2628 1425 1525
Fragments arranged by size:
2628 1525 1425 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7109 7109
Size: 7114
BlpI GC'TnA_GC
Cuts at: 2249 2349 2444 4203 4531 2249
Size: 100 95 1759 328 4832
Fragments arranged by size:
4832 1759 328 100 95
Bme1580I G_kGCm'C
Cuts at: 1790 2187 2825 3240 3350 3423 3915 5175 6331
Size: 397 638 415 110 73 492 1260 1156
Cuts at: 6331 1790
Size: 2573
Fragments arranged by size:
2573 1260 1156 638 492 415 397 110
73
BmgBI CAC'GTC
Cuts at: 1318 2179 3565 1318
Size: 861 1386 4867
Fragments arranged by size:
4867 1386 861
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4845 5803 442
Size: 416 53 83 399 24 3428 958 1753
Fragments arranged by size:
3428 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2863 2863
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2246 2474 2795 3335 3852 3962 5774 1239
Size: 1007 228 321 540 517 110 1812 2579
Fragments arranged by size:
2579 1812 1007 540 517 321 228 110
Bpu10I CC'TnA_GC
Cuts at: 2255 2634 2748 2869 4350 2255
Size: 379 114 121 1481 5019
Fragments arranged by size:
5019 1481 379 121 114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2321 2483 2579 3203 3410 5239 6104 6402 2321
Size: 162 96 624 207 1829 865 298 3033
Fragments arranged by size:
3033 1829 865 624 298 207 162 96
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3311 4367 5777 882
Size: 617 1812 1056 1410 2219
Fragments arranged by size:
2219 1812 1410 1056 617
BsaAI yAC'GTr
Cuts at: 493 1204 3511 3649 3901 4290 4905 493
Size: 711 2307 138 252 389 615 2702
Fragments arranged by size:
2702 2307 711 615 389 252 138
BsaBI GATnn'nnATC
Cuts at: 3407 7108 3407
Size: 3701 3413
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4921 5303 5938 275
Size: 53 83 186 936 3388 382 635 1451
Fragments arranged by size:
3388 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5547 5945 6219 6366 6777 5547
Size: 398 274 147 411 5884
Fragments arranged by size:
5884 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2219 2249 3329 3359 3878
Size: 30 165 30 816 30 1080 30 519
Cuts at: 3878 3908 4047 4077 1178
Size: 30 139 30 4215
Fragments arranged by size:
4215 1080 816 519 165 139 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 2519 2714 2732 2741 2744 2774 2810
Size: 1249 84 195 18 9 3 30 36
Cuts at: 2810 2825 3113 3965 1186
Size: 15 288 852 4335
Fragments arranged by size:
4335 1249 852 288 195 84 36 30
18 15 9 3
BseYI C'CCAG_C
Cuts at: 2016 2062 2486 2769 4316 6317 6813 2016
Size: 46 424 283 1547 2001 496 2317
Fragments arranged by size:
2317 2001 1547 496 424 283 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2562 3040 3125 3787 3796 4142
Size: 573 329 497 478 85 662 9 346
Cuts at: 4142 1163
Size: 4135
Fragments arranged by size:
4135 662 573 497 478 346 329 85
9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2887 3045 3277 4564 5325 6353
Size: 275 186 886 158 232 1287 761 1028
Cuts at: 6353 1540
Size: 2301
Fragments arranged by size:
2301 1287 1028 886 761 275 232 186
158
BsiHKAI G_wGCw'C
Cuts at: 2187 2595 2657 2844 3240 3342 3915 5175 5260
Size: 408 62 187 396 102 573 1260 85
Cuts at: 5260 6331 2187
Size: 1071 2970
Fragments arranged by size:
2970 1260 1071 573 408 396 187 102
85 62
BsmI GAATG_Cn'
Cuts at: 3570 4625 4718 3570
Size: 1055 93 5966
Fragments arranged by size:
5966 1055 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4070 4070
Size: 7114
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2261 2595 2657 2682 2825 2844
Size: 399 397 74 334 62 25 143 19
Cuts at: 2844 3240 3342 3350 3423 3915 3948 4433 5175
Size: 396 102 8 73 492 33 485 742
Cuts at: 5175 5260 6331 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 485 399 397 396
334 143 102 85 74 73 62 33
25 19 8
BspEI T'CCGG_A
Cuts at: 6777 6777
Size: 7114
BspHI T'CATG_A
Cuts at: 5003 6733 5003
Size: 1730 5384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2209 3147 3897 4294 4469 844
Size: 1365 938 750 397 175 3489
Fragments arranged by size:
3489 1365 938 750 397 175
BsrBI CCG'CTC
Cuts at: 1394 1823 3279 5001 1394
Size: 429 1456 1722 3507
Fragments arranged by size:
3507 1722 1456 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5609 5783 65
Size: 1183 4361 174 1396
Fragments arranged by size:
4361 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2353 4048 5758 5945 1700
Size: 110 543 1695 1710 187 2869
Fragments arranged by size:
2869 1710 1695 543 187 110
BsrGI T'GTAC_A
Cuts at: 96 2235 96
Size: 2139 4975
BssHII G'CGCG_C
Cuts at: 1925 3167 1925
Size: 1242 5872
BssSI C'ACGA_G
Cuts at: 2608 3007 5174 6186 2608
Size: 399 2167 1012 3536
Fragments arranged by size:
3536 2167 1012 399
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3522 3774 4309 794
Size: 2728 252 535 3599
Fragments arranged by size:
3599 2728 535 252
BstBI TT'CG_AA
Cuts at: 5918 5918
Size: 7114
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7114
BstXI CCAn_nnnn'nTGG
Cuts at: 3721 4198 3721
Size: 477 6637
BstYI r'GATC_y
Cuts at: 1972 3402 3486 3936 4444 4811 5196 5213 6665
Size: 1430 84 450 508 367 385 17 1452
Cuts at: 6665 6751 6763 7109 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1430 508 450 385 367 346
86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 3460 3460
Size: 7114
BtgI C'CryG_G
Cuts at: 513 1065 2004 2286 513
Size: 552 939 282 5341
Fragments arranged by size:
5341 939 552 282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3394 3625 487
Size: 683 56 2168 231 3976
Fragments arranged by size:
3976 2168 683 231 56
BtsI GCAGTG_nn'
Cuts at: 2130 3352 3520 3964 4068 4630 5424 5444 2130
Size: 1222 168 444 104 562 794 20 3800
Fragments arranged by size:
3800 1222 794 562 444 168 104 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7079
DraI TTT'AAA
Cuts at: 4418 4418
Size: 7114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6121 809
Size: 507 4805 1802
Fragments arranged by size:
4805 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2241 2283 2289
Size: 54 1425 50 275 18 411 42 6
Cuts at: 2289 2388 2430 2532 3042 3274 3723 4561 5450
Size: 99 42 102 510 232 449 838 889
Cuts at: 5450 7079 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 838 510 449 411 275
232 102 99 54 50 43 42 42
18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3042 3274 4561 1537
Size: 275 1230 232 1287 4090
Fragments arranged by size:
4090 1287 1230 275 232
EarI CTCTTCn'nnn_
Cuts at: 1353 2165 2691 3104 3203 5044 1353
Size: 812 526 413 99 1841 3423
Fragments arranged by size:
3423 1841 812 526 413 99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3743 4322 5701 6075 6221 387
Size: 1064 138 2154 579 1379 374 146 1280
Fragments arranged by size:
2154 1379 1280 1064 579 374 146 138
EcoICRI GAG'CTC
Cuts at: 2593 2655 2842 2593
Size: 62 187 6865
Fragments arranged by size:
6865 187 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2246 2474 2795 3101 3335 3407 3419
Size: 393 614 228 321 306 234 72 12
Cuts at: 3419 3491 3852 3962 4109 5177 5774 6561 6829
Size: 72 361 110 147 1068 597 787 268
Cuts at: 6829 1239
Size: 1524
Fragments arranged by size:
1524 1068 787 614 597 393 361 321
306 268 234 228 147 110 72 72
12
EcoNI CCTnn'n_nnAGG
Cuts at: 2216 2501 2216
Size: 285 6829
EcoO109I rG'GnC_Cy
Cuts at: 2483 2494 2788 2860 3420 3802 2483
Size: 11 294 72 560 382 5795
Fragments arranged by size:
5795 560 382 294 72 11
EcoRI G'AATT_C
Cuts at: 4423 4801 4423
Size: 378 6736
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2176 2176
Size: 7114
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 3226 3552 3691 6261 1678
Size: 1548 326 139 2570 2531
Fragments arranged by size:
2570 2531 1548 326 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5755 5787 5829 5861 1373
Size: 32 135 32 4183 32 42 32 2626
Fragments arranged by size:
4183 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4458 4704 669
Size: 988 281 2520 246 3079
Fragments arranged by size:
3079 2520 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7114
HpaI GTT'AAC
Cuts at: 4704 4704
Size: 7114
KpnI G_GTAC'C
Cuts at: 4439 4439
Size: 7114
MfeI C'AATT_G
Cuts at: 4713 4713
Size: 7114
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3060 3312 6228 6412 1305
Size: 1755 252 2916 184 2007
Fragments arranged by size:
2916 2007 1755 252 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2291 2390 2432 3725 10
Size: 54 1425 343 459 99 42 1293 3399
Fragments arranged by size:
3399 1425 1293 459 343 99 54 42
MslI CAynn'nnrTG
Cuts at: 518 1166 2087 3579 3595 3763 4043 5072 5431
Size: 648 921 1492 16 168 280 1029 359
Cuts at: 5431 5590 518
Size: 159 2042
Fragments arranged by size:
2042 1492 1029 921 648 359 280 168
159 16
MspA1I CmG'CkG
Cuts at: 1606 2044 2056 2062 2538 2747 2773 2819 3177
Size: 438 12 6 476 209 26 46 358
Cuts at: 3177 3232 3243 3495 3559 4530 5207 6355 6600
Size: 55 11 252 64 971 677 1148 245
Cuts at: 6600 1606
Size: 2120
Fragments arranged by size:
2120 1148 971 677 476 438 358 252
245 209 64 55 46 26 12 11
6
NaeI GCC'GGC
Cuts at: 2355 2355
Size: 7114
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5623
Fragments arranged by size:
5623 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7114
NgoMIV G'CCGG_C
Cuts at: 2353 2353
Size: 7114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7114
NotI GC'GGCC_GC
Cuts at: 3274 4561 3274
Size: 1287 5827
NruI TCG'CGA
Cuts at: 4341 4341
Size: 7114
NspI r_CATG'y
Cuts at: 1132 1229 2033 4211 4472 6017 1132
Size: 97 804 2178 261 1545 2229
Fragments arranged by size:
2229 2178 1545 804 261 97
PasI CC'CwG_GG
Cuts at: 1348 2366 1348
Size: 1018 6096
PciI A'CATG_T
Cuts at: 6013 6013
Size: 7114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3232 4234 1108
Size: 2124 1002 3988
Fragments arranged by size:
3988 2124 1002
PfoI T'CCnGG_A
Cuts at: 3939 3939
Size: 7114
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PmlI CAC'GTG
Cuts at: 3649 3901 4290 3649
Size: 252 389 6473
Fragments arranged by size:
6473 389 252
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5183 5215 6696 6728 5183
Size: 32 1481 32 5569
Fragments arranged by size:
5569 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2494 3802 2494
Size: 1308 5806
PshAI GACnn'nnGTC
Cuts at: 1613 4025 4082 1613
Size: 2412 57 4645
Fragments arranged by size:
4645 2412 57
PsiI TTA'TAA
Cuts at: 4684 4684
Size: 7114
PspOMI G'GGCC_C
Cuts at: 3419 3419
Size: 7114
PstI C_TGCA'G
Cuts at: 830 2141 2629 3558 4063 4314 4466 830
Size: 1311 488 929 505 251 152 3478
Fragments arranged by size:
3478 1311 929 505 488 251 152
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7114
PvuII CAG'CTG
Cuts at: 2044 2062 2747 2773 3232 2044
Size: 18 685 26 459 5926
Fragments arranged by size:
5926 685 459 26 18
SacI G_AGCT'C
Cuts at: 2595 2657 2844 2595
Size: 62 187 6865
Fragments arranged by size:
6865 187 62
SalI G'TCGA_C
Cuts at: 1936 4456 1936
Size: 2520 4594
SapI GCTCTTCn'nnn_
Cuts at: 2165 3104 3203 2165
Size: 939 99 6076
Fragments arranged by size:
6076 939 99
SbfI CC_TGCA'GG
Cuts at: 4466 4466
Size: 7114
ScaI AGT'ACT
Cuts at: 2147 5362 7074 2147
Size: 3215 1712 2187
Fragments arranged by size:
3215 2187 1712
SexAI A'CCwGG_T
Cuts at: 3372 3372
Size: 7114
SfcI C'TryA_G
Cuts at: 826 1045 2137 2625 3554 4059 4310 4383 4462
Size: 219 1092 488 929 505 251 73 79
Cuts at: 4462 5597 6278 6469 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1092 929 681 505 488 251
219 191 79 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7114
SmaI CCC'GGG
Cuts at: 1872 2573 2858 4441 1872
Size: 701 285 1583 4545
Fragments arranged by size:
4545 1583 701 285
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2336 2498 2594 3218 3425 5218
Size: 197 926 393 162 96 624 207 1793
Cuts at: 5218 6119 6381 820
Size: 901 262 1553
Fragments arranged by size:
1793 1553 926 901 624 393 262 207
197 162 96
SnaBI TAC'GTA
Cuts at: 493 4905 493
Size: 4412 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 4472 1132
Size: 3340 3774
SspI AAT'ATT
Cuts at: 5 52 5038 5
Size: 47 4986 2081
Fragments arranged by size:
4986 2081 47
StuI AGG'CCT
Cuts at: 1517 2211 2384 2903 3029 3617 3785 1517
Size: 694 173 519 126 588 168 4846
Fragments arranged by size:
4846 694 588 519 173 168 126
StyI C'CwwG_G
Cuts at: 513 1065 2004 2385 2898 4553 513
Size: 552 939 381 513 1655 3074
Fragments arranged by size:
3074 1655 939 552 513 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2491 3638 5141 5158 5311 5496 5941
Size: 1255 911 1147 1503 17 153 185 445
Cuts at: 5941 325
Size: 1498
Fragments arranged by size:
1503 1498 1255 1147 911 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 2235 5360
Size: 274 80 33 51 1443 168 90 3125
Cuts at: 5360 7072 96
Size: 1712 138
Fragments arranged by size:
3125 1712 1443 274 168 138 90 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3329 3361 4047 4079 7074 7106 3329
Size: 32 686 32 2995 32 3337
Fragments arranged by size:
3337 2995 686 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3989 1316
Size: 2673 4441
XbaI T'CTAG_A
Cuts at: 4450 4450
Size: 7114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2614 4287 2614
Size: 1673 5441
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7114
XmaI C'CCGG_G
Cuts at: 1870 2571 2856 4439 1870
Size: 701 285 1583 4545
Fragments arranged by size:
4545 1583 701 285
XmnI GAAnn'nnTTC
Cuts at: 2945 3002 3449 5243 2945
Size: 57 447 1794 4816
Fragments arranged by size:
4816 1794 447 57
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4922 276
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I
DraIII EcoRV FseI FspAI KasI NarI NsiI PacI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI