Restriction map for
pFN21AB0407
(Circular) (Six-base) MAPSORT of: pFN21AB0407.seq Check: 5262 from: 1 to: 6100
pFN21AB0407 6100 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2516 3065 2516
Size: 549 5551
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2736 3910 278
Size: 53 83 186 936 1200 1174 2468
Fragments arranged by size:
2468 1200 1174 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3443 1937
Size: 1506 4594
Acc65I G'GTAC_C
Cuts at: 2946 3421 2946
Size: 475 5625
AclI AA'CG_TT
Cuts at: 1563 4227 4600 1563
Size: 2664 373 3063
Fragments arranged by size:
3063 2664 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2899 4163 5547 5815 1632
Size: 1267 1264 1384 268 1917
Fragments arranged by size:
1917 1384 1267 1264 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5903
AflIII A'CryG_T
Cuts at: 1203 4999 5976 1203
Size: 3796 977 1327
Fragments arranged by size:
3796 1327 977
AgeI A'CCGG_T
Cuts at: 2943 4931 2943
Size: 1988 4112
AhdI GACnn_n'nnGTC
Cuts at: 2538 4829 2538
Size: 2291 3809
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6100
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2976 1184
Size: 1792 4308
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3374 3406 3374
Size: 32 6068
AlwNI CAG_nnn'CTG
Cuts at: 2508 3843 5415 2508
Size: 1335 1572 3193
Fragments arranged by size:
3193 1572 1335
ApaLI G'TGCA_C
Cuts at: 2725 4157 5313 2725
Size: 1432 1156 3512
Fragments arranged by size:
3512 1432 1156
ApoI r'AATT_y
Cuts at: 1493 2624 3409 3635 3787 5896 1493
Size: 1131 785 226 152 2109 1697
Fragments arranged by size:
2109 1697 1131 785 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4654 160
Size: 872 3622 1606
Fragments arranged by size:
3622 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
AvaI C'yCGr_G
Cuts at: 1870 1943 2313 3332 3425 1870
Size: 73 370 1019 93 4545
Fragments arranged by size:
4545 1019 370 93 73
BamHI G'GATC_C
Cuts at: 3430 3797 3430
Size: 367 5733
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2946 3421 4876 618
Size: 325 217 627 1159 475 1455 1842
Fragments arranged by size:
1842 1455 1159 627 475 325 217
BanII G_rGCy'C
Cuts at: 1391 2301 2744 2907 3419 1391
Size: 910 443 163 512 4072
Fragments arranged by size:
4072 910 512 443 163
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5129
BbvCI CC'TCA_GC
Cuts at: 2153 2324 2153
Size: 171 5929
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4291 4325 1903
Size: 34 2354 34 3678
Fragments arranged by size:
3678 2354 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3992 5202 3992
Size: 1210 4890
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6100
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3203 4711 136
Size: 107 122 71 631 605 1531 1508 1525
Fragments arranged by size:
1531 1525 1508 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6095 6095
Size: 6100
BlpI GC'TnA_GC
Cuts at: 2097 3517 2097
Size: 1420 4680
Bme1580I G_kGCm'C
Cuts at: 1790 2729 4161 5317 1790
Size: 939 1432 1156 2573
Fragments arranged by size:
2573 1432 1156 939
BmgBI CAC'GTC
Cuts at: 1318 3397 1318
Size: 2079 4021
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2039 3316 3831
Size: 416 53 83 399 24 622 1277 515
Cuts at: 3831 4789 442
Size: 958 1753
Fragments arranged by size:
1753 1277 958 622 515 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6100
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2328 2328
Size: 6100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2014 2274 4760 1239
Size: 775 260 2486 2579
Fragments arranged by size:
2579 2486 775 260
Bpu10I CC'TnA_GC
Cuts at: 2153 2324 2153
Size: 171 5929
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2066 4225 5090 5388 2066
Size: 2159 865 298 2778
Fragments arranged by size:
2778 2159 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2212 4763 882
Size: 617 713 2551 2219
Fragments arranged by size:
2551 2219 713 617
BsaAI yAC'GTr
Cuts at: 493 1204 2724 2776 3891 493
Size: 711 1520 52 1115 2702
Fragments arranged by size:
2702 1520 1115 711 52
BsaBI GATnn'nnATC
Cuts at: 6094 6094
Size: 6100
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2733 3907 4289 4924
Size: 53 83 186 936 1200 1174 382 635
Cuts at: 4924 275
Size: 1451
Fragments arranged by size:
1451 1200 1174 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2574 2943 4533 4931 5205 5352 5763 2574
Size: 369 1590 398 274 147 411 2911
Fragments arranged by size:
2911 1590 411 398 369 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2218 2248 2337 2367 1178
Size: 30 165 30 815 30 89 30 4911
Fragments arranged by size:
4911 815 165 89 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2981 1186
Size: 1795 4305
BseYI C'CCAG_C
Cuts at: 2550 5303 5799 2550
Size: 2753 496 2851
Fragments arranged by size:
2851 2753 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2192 2234 2357 2495 2521 2640 2982
Size: 573 456 42 123 138 26 119 342
Cuts at: 2982 3154 3346 1163
Size: 172 192 3917
Fragments arranged by size:
3917 573 456 342 192 172 138 123
119 42 26
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3550 4311 5339 1540
Size: 275 186 1549 761 1028 2301
Fragments arranged by size:
2301 1549 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2647 2729 2907 4161 4246 5317 2647
Size: 82 178 1254 85 1071 3430
Fragments arranged by size:
3430 1254 1071 178 85 82
BsmI GAATG_Cn'
Cuts at: 2251 2594 3611 3704 2251
Size: 343 1017 93 4647
Fragments arranged by size:
4647 1017 343 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2301 2647 2729 2744 2907 3419 4161
Size: 399 511 346 82 15 163 512 742
Cuts at: 4161 4246 5317 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 512 511 399 346 163
85 82 15
BspEI T'CCGG_A
Cuts at: 5763 5763
Size: 6100
BspHI T'CATG_A
Cuts at: 3989 5719 3989
Size: 1730 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2408 2516 3065 3455 844
Size: 1564 108 549 390 3489
Fragments arranged by size:
3489 1564 549 390 108
BsrBI CCG'CTC
Cuts at: 1394 1823 2293 3020 3987 1394
Size: 429 470 727 967 3507
Fragments arranged by size:
3507 967 727 470 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3076 4595 4769 65
Size: 1183 1828 1519 174 1396
Fragments arranged by size:
1828 1519 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2943 4744 4931 1700
Size: 110 1133 1801 187 2869
Fragments arranged by size:
2869 1801 1133 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6100
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6100
BssSI C'ACGA_G
Cuts at: 4160 5172 4160
Size: 1012 5088
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2508 2724 794
Size: 1714 216 4170
Fragments arranged by size:
4170 1714 216
BstBI TT'CG_AA
Cuts at: 4904 4904
Size: 6100
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6100
BstXI CCAn_nnnn'nTGG
Cuts at: 2550 2957 2550
Size: 407 5693
BstYI r'GATC_y
Cuts at: 1972 3430 3797 4182 4199 5651 5737 5749 6095
Size: 1458 367 385 17 1452 86 12 346
Cuts at: 6095 1972
Size: 1977
Fragments arranged by size:
1977 1458 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3154 3154
Size: 6100
BtgI C'CryG_G
Cuts at: 513 1065 2035 2222 513
Size: 552 970 187 4391
Fragments arranged by size:
4391 970 552 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5361
Fragments arranged by size:
5361 683 56
BtsI GCAGTG_nn'
Cuts at: 2602 3142 3616 4410 4430 2602
Size: 540 474 794 20 4272
Fragments arranged by size:
4272 794 540 474 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6100
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2762 2797 529
Size: 35 2198 35 3832
Fragments arranged by size:
3832 2198 35 35
DraI TTT'AAA
Cuts at: 2635 3404 2635
Size: 769 5331
DraIII CAC_nnn'GTG
Cuts at: 2212 2212
Size: 6100
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5107 809
Size: 507 3791 1802
Fragments arranged by size:
3791 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3547 4436 6065
Size: 54 1425 50 275 18 1717 889 1629
Cuts at: 6065 8
Size: 43
Fragments arranged by size:
1717 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3547 1537
Size: 275 1735 4090
Fragments arranged by size:
4090 1735 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2925 4030 1353
Size: 1572 1105 3423
Fragments arranged by size:
3423 1572 1105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4687 5061 5207 387
Size: 1064 138 3098 374 146 1280
Fragments arranged by size:
3098 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2905 2905
Size: 6100
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2014 2274 2899 4163 4760 5547 5815
Size: 393 382 260 625 1264 597 787 268
Cuts at: 5815 1239
Size: 1524
Fragments arranged by size:
1524 1264 787 625 597 393 382 268
260
EcoO109I rG'GnC_Cy
Cuts at: 2459 2459
Size: 6100
EcoRI G'AATT_C
Cuts at: 3409 3787 3409
Size: 378 5722
FspI TGC'GCA
Cuts at: 4606 4606
Size: 6100
HaeII r_GCGC'y
Cuts at: 1678 5247 1678
Size: 3569 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2218 2250 2524 2556 4741
Size: 32 135 32 646 32 274 32 2185
Cuts at: 4741 4773 4815 4847 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2185 646 274 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3444 3690 669
Size: 988 281 1506 246 3079
Fragments arranged by size:
3079 1506 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6100
HpaI GTT'AAC
Cuts at: 3690 3690
Size: 6100
KpnI G_GTAC'C
Cuts at: 2950 3425 2950
Size: 475 5625
MfeI C'AATT_G
Cuts at: 3699 3699
Size: 6100
MluI A'CGCG_T
Cuts at: 5976 5976
Size: 6100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2684 5214 5398 1305
Size: 1379 2530 184 2007
Fragments arranged by size:
2530 2007 1379 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4278
Fragments arranged by size:
4278 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3302 4058 4417 4576 518
Size: 648 843 1293 756 359 159 2042
Fragments arranged by size:
2042 1293 843 756 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2405 3264 3516 4193 5341 5586 1606
Size: 799 859 252 677 1148 245 2120
Fragments arranged by size:
2120 1148 859 799 677 252 245
NcoI C'CATG_G
Cuts at: 513 1065 2035 513
Size: 552 970 4578
Fragments arranged by size:
4578 970 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6100
NotI GC'GGCC_GC
Cuts at: 3547 3547
Size: 6100
NspI r_CATG'y
Cuts at: 1132 1229 3301 3458 5003 1132
Size: 97 2072 157 1545 2229
Fragments arranged by size:
2229 2072 1545 157 97
PasI CC'CwG_GG
Cuts at: 1348 2536 2609 1348
Size: 1188 73 4839
Fragments arranged by size:
4839 1188 73
PciI A'CATG_T
Cuts at: 4999 4999
Size: 6100
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2405 1108
Size: 1297 4803
PmeI GTTT'AAAC
Cuts at: 3404 3404
Size: 6100
PmlI CAC'GTG
Cuts at: 2776 2776
Size: 6100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3151 3183 4169 4201 5682 5714 3151
Size: 32 986 32 1481 32 3537
Fragments arranged by size:
3537 1481 986 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6100
PsiI TTA'TAA
Cuts at: 3670 3670
Size: 6100
PspXI vC'TCGA_Gb
Cuts at: 3332 3332
Size: 6100
PstI C_TGCA'G
Cuts at: 830 2513 2982 3452 830
Size: 1683 469 470 3478
Fragments arranged by size:
3478 1683 470 469
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6100
PvuII CAG'CTG
Cuts at: 2405 2405
Size: 6100
SacI G_AGCT'C
Cuts at: 2907 2907
Size: 6100
SalI G'TCGA_C
Cuts at: 1936 3442 1936
Size: 1506 4594
SapI GCTCTTCn'nnn_
Cuts at: 2925 2925
Size: 6100
SbfI CC_TGCA'GG
Cuts at: 2513 3452 2513
Size: 939 5161
ScaI AGT'ACT
Cuts at: 4348 6060 4348
Size: 1712 4388
SfcI C'TryA_G
Cuts at: 826 1045 2509 2978 3175 3448 4583 5264 5455
Size: 219 1464 469 197 273 1135 681 191
Cuts at: 5455 826
Size: 1471
Fragments arranged by size:
1471 1464 1135 681 469 273 219 197
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6100
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6100
SmaI CCC'GGG
Cuts at: 1872 3427 1872
Size: 1555 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2081 3332 4204 5105 5367 820
Size: 197 926 138 1251 872 901 262 1553
Fragments arranged by size:
1553 1251 926 901 872 262 197 138
SnaBI TAC'GTA
Cuts at: 493 3891 493
Size: 3398 2702
SpeI A'CTAG_T
Cuts at: 152 2615 152
Size: 2463 3637
SphI G_CATG'C
Cuts at: 1132 3301 3458 1132
Size: 2169 157 3774
Fragments arranged by size:
3774 2169 157
SspI AAT'ATT
Cuts at: 5 52 4024 5
Size: 47 3972 2081
Fragments arranged by size:
3972 2081 47
StuI AGG'CCT
Cuts at: 1517 3173 1517
Size: 1656 4444
StyI C'CwwG_G
Cuts at: 513 1065 2035 3539 513
Size: 552 970 1504 3074
Fragments arranged by size:
3074 1504 970 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4127 4144 4297 4482 4927 325
Size: 1255 2547 17 153 185 445 1498
Fragments arranged by size:
2547 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2937 4346 6058
Size: 274 80 33 51 1443 960 1409 1712
Cuts at: 6058 96
Size: 138
Fragments arranged by size:
1712 1443 1409 960 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2337 2369 2569 2601 6060 6092 2337
Size: 32 200 32 3459 32 2345
Fragments arranged by size:
3459 2345 200 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6100
XbaI T'CTAG_A
Cuts at: 3436 3436
Size: 6100
XhoI C'TCGA_G
Cuts at: 1943 3332 1943
Size: 1389 4711
XmaI C'CCGG_G
Cuts at: 1870 3425 1870
Size: 1555 4545
XmnI GAAnn'nnTTC
Cuts at: 4229 4229
Size: 6100
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2734 3908 276
Size: 53 83 186 936 1200 1174 2468
Fragments arranged by size:
2468 1200 1174 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI
PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI