Restriction map for
pFN21AB0404
(Circular) (Six-base) MAPSORT of: pFN21AB0404.seq Check: 838 from: 1 to: 6541
pFN21AB0404 6541 bp
With 182 enzymes: SgfI *
August 16, 2010 14:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2284 2885 2284
Size: 601 5940
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4351 278
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3884 1937
Size: 1947 4594
Acc65I G'GTAC_C
Cuts at: 3862 3862
Size: 6541
AclI AA'CG_TT
Cuts at: 1563 4668 5041 1563
Size: 3105 373 3063
Fragments arranged by size:
3105 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2360 3464 3660 3669 3750 4604 5988 6256
Size: 728 1104 196 9 81 854 1384 268
Cuts at: 6256 1632
Size: 1917
Fragments arranged by size:
1917 1384 1104 854 728 268 196 81
9
AfeI AGC'GCT
Cuts at: 2213 2213
Size: 6541
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6344
AflIII A'CryG_T
Cuts at: 1203 5440 6417 1203
Size: 4237 977 1327
Fragments arranged by size:
4237 1327 977
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 5270 5270
Size: 6541
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6541
AlwNI CAG_nnn'CTG
Cuts at: 3035 3301 4284 5856 3035
Size: 266 983 1572 3720
Fragments arranged by size:
3720 1572 983 266
ApaLI G'TGCA_C
Cuts at: 3657 4598 5754 3657
Size: 941 1156 4444
Fragments arranged by size:
4444 1156 941
ApoI r'AATT_y
Cuts at: 1493 2366 3308 3830 3850 4076 4228 6337 1493
Size: 873 942 522 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 942 873 522 226 152 20
AseI AT'TA_AT
Cuts at: 160 1032 5095 160
Size: 872 4063 1606
Fragments arranged by size:
4063 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 2565 3866 1870
Size: 73 622 1301 4545
Fragments arranged by size:
4545 1301 622 73
BamHI G'GATC_C
Cuts at: 2295 3330 3871 4238 2295
Size: 1035 541 367 4598
Fragments arranged by size:
4598 1035 541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2878 2986 3718 3862 5317
Size: 325 217 627 1091 108 732 144 1455
Cuts at: 5317 618
Size: 1842
Fragments arranged by size:
1842 1455 1091 732 627 325 217 144
108
BanII G_rGCy'C
Cuts at: 1391 2315 2643 2906 3797 3860 1391
Size: 924 328 263 891 63 4072
Fragments arranged by size:
4072 924 891 328 263 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2348 928
Size: 971 449 5121
Fragments arranged by size:
5121 971 449
BbvCI CC'TCA_GC
Cuts at: 2994 2994
Size: 6541
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4732 4766 1903
Size: 34 2795 34 3678
Fragments arranged by size:
3678 2795 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4433 5643 4433
Size: 1210 5331
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6541
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5152 136
Size: 107 122 71 631 605 337 3143 1525
Fragments arranged by size:
3143 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2836 3699 6536 2836
Size: 863 2837 2841
Fragments arranged by size:
2841 2837 863
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 3661 3815 4602 5758 1790
Size: 1871 154 787 1156 2573
Fragments arranged by size:
2573 1871 1156 787 154
BmgBI CAC'GTC
Cuts at: 1318 2182 1318
Size: 864 5677
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2473 2709 3311
Size: 416 53 83 399 24 1056 236 602
Cuts at: 3311 4272 5230 442
Size: 961 958 1753
Fragments arranged by size:
1753 1056 961 958 602 416 399 236
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2343 3374 2343
Size: 1031 5510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2256 3536 3783 3788 5201 1239
Size: 884 133 1280 247 5 1413 2579
Fragments arranged by size:
2579 1413 1280 884 247 133 5
Bpu10I CC'TnA_GC
Cuts at: 2994 2994
Size: 6541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3677 4666 5531 5829 3677
Size: 989 865 298 4389
Fragments arranged by size:
4389 989 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2855 3345 5204 882
Size: 617 1356 490 1859 2219
Fragments arranged by size:
2219 1859 1356 617 490
BsaAI yAC'GTr
Cuts at: 493 1204 4332 493
Size: 711 3128 2702
Fragments arranged by size:
3128 2702 711
BsaBI GATnn'nnATC
Cuts at: 2135 6535 2135
Size: 4400 2141
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2380 4348 4730 5365
Size: 53 83 186 936 847 1968 382 635
Cuts at: 5365 275
Size: 1451
Fragments arranged by size:
1968 1451 936 847 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4974 5372 5646 5793 6204 4974
Size: 398 274 147 411 5311
Fragments arranged by size:
5311 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6316
Fragments arranged by size:
6316 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2120 2185 2279 2667 2690 2777 2840 2996
Size: 934 65 94 388 23 87 63 156
Cuts at: 2996 3011 3128 3266 1186
Size: 15 117 138 4461
Fragments arranged by size:
4461 934 388 156 138 117 94 87
65 63 23 15
BseYI C'CCAG_C
Cuts at: 5744 6240 5744
Size: 496 6045
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2622 2706 3086 1163
Size: 573 886 84 380 4618
Fragments arranged by size:
4618 886 573 380 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2629 3991 4752 5780 1540
Size: 275 186 628 1362 761 1028 2301
Fragments arranged by size:
2301 1362 1028 761 628 275 186
BsiHKAI G_wGCw'C
Cuts at: 3043 3661 4602 4687 5758 3043
Size: 618 941 85 1071 3826
Fragments arranged by size:
3826 1071 941 618 85
BsmI GAATG_Cn'
Cuts at: 4052 4145 4052
Size: 93 6448
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2315 2643 2906 3043 3661 3797 3815
Size: 399 525 328 263 137 618 136 18
Cuts at: 3815 3860 4602 4687 5758 1391
Size: 45 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 618 525 399 328 263
137 136 85 45 18
BspEI T'CCGG_A
Cuts at: 6204 6204
Size: 6541
BspHI T'CATG_A
Cuts at: 4430 6160 4430
Size: 1730 4811
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2284 2594 2885 3058 3896 844
Size: 1440 310 291 173 838 3489
Fragments arranged by size:
3489 1440 838 310 291 173
BsrBI CCG'CTC
Cuts at: 1394 1823 3388 3460 4428 1394
Size: 429 1565 72 968 3507
Fragments arranged by size:
3507 1565 968 429 72
BsrDI GCAATG_nn'
Cuts at: 65 1248 2539 3477 5036 5210 65
Size: 1183 1291 938 1559 174 1396
Fragments arranged by size:
1559 1396 1291 1183 938 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2078 2989 3354 5185 5372 1700
Size: 110 268 911 365 1831 187 2869
Fragments arranged by size:
2869 1831 911 365 268 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 3682 4601 5613 3682
Size: 919 1012 4610
Fragments arranged by size:
4610 1012 919
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6541
BstBI TT'CG_AA
Cuts at: 3082 5345 3082
Size: 2263 4278
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6541
BstXI CCAn_nnnn'nTGG
Cuts at: 2291 2528 2291
Size: 237 6304
BstYI r'GATC_y
Cuts at: 1972 2136 2295 2836 3330 3699 3871 4238 4623
Size: 164 159 541 494 369 172 367 385
Cuts at: 4623 4640 6092 6178 6190 6536 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 541 494 385 369 367 346
172 164 159 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2760 3015 2760
Size: 255 6286
BtgI C'CryG_G
Cuts at: 513 1065 2004 3632 513
Size: 552 939 1628 3422
Fragments arranged by size:
3422 1628 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5802
Fragments arranged by size:
5802 683 56
BtsI GCAGTG_nn'
Cuts at: 4057 4851 4871 4057
Size: 794 20 5727
Fragments arranged by size:
5727 794 20
ClaI AT'CG_AT
Cuts at: 1554 2625 1554
Size: 1071 5470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6506
DraI TTT'AAA
Cuts at: 3845 3845
Size: 6541
DraIII CAC_nnn'GTG
Cuts at: 2717 2717
Size: 6541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5548 809
Size: 507 4232 1802
Fragments arranged by size:
4232 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3988 4877 6506
Size: 54 1425 50 275 18 2158 889 1629
Cuts at: 6506 8
Size: 43
Fragments arranged by size:
2158 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3988 1537
Size: 275 2176 4090
Fragments arranged by size:
4090 2176 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3243 3645 3726 4471 1353
Size: 1890 402 81 745 3423
Fragments arranged by size:
3423 1890 745 402 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2027 3617 5128 5502 5648 387
Size: 1064 138 438 1590 1511 374 146 1280
Fragments arranged by size:
1590 1511 1280 1064 438 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2123 2256 2360 3464 3536 3660 3669
Size: 393 491 133 104 1104 72 124 9
Cuts at: 3669 3750 3783 3788 4604 5201 5988 6256 1239
Size: 81 33 5 816 597 787 268 1524
Fragments arranged by size:
1524 1104 816 787 597 491 393 268
133 124 104 81 72 33 9 5
EcoO109I rG'GnC_Cy
Cuts at: 2288 2562 2843 2968 3004 3820 2288
Size: 274 281 125 36 816 5009
Fragments arranged by size:
5009 816 281 274 125 36
EcoRI G'AATT_C
Cuts at: 2366 3850 4228 2366
Size: 1484 378 4679
Fragments arranged by size:
4679 1484 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3718 3718
Size: 6541
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 2215 5688 1678
Size: 537 3473 2531
Fragments arranged by size:
3473 2531 537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2370 2402 2630 2662 2802
Size: 32 135 32 798 32 228 32 140
Cuts at: 2802 2834 2852 2884 5182 5214 5256 5288 1373
Size: 32 18 32 2298 32 42 32 2626
Fragments arranged by size:
2626 2298 798 228 140 135 42 32
32 32 32 32 32 32 32 18
HincII GTy'rAC
Cuts at: 669 1657 1938 3885 4131 669
Size: 988 281 1947 246 3079
Fragments arranged by size:
3079 1947 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6541
HpaI GTT'AAC
Cuts at: 4131 4131
Size: 6541
KpnI G_GTAC'C
Cuts at: 3866 3866
Size: 6541
MfeI C'AATT_G
Cuts at: 4140 4140
Size: 6541
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5655 5839 1305
Size: 4350 184 2007
Fragments arranged by size:
4350 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4719
Fragments arranged by size:
4719 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4499 4858 5017 518
Size: 648 3333 359 159 2042
Fragments arranged by size:
3333 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2234 3754 3957 4634 5782 6027 1606
Size: 628 1520 203 677 1148 245 2120
Fragments arranged by size:
2120 1520 1148 677 628 245 203
NaeI GCC'GGC
Cuts at: 2991 2991
Size: 6541
NcoI C'CATG_G
Cuts at: 513 1065 2004 3632 513
Size: 552 939 1628 3422
Fragments arranged by size:
3422 1628 939 552
NdeI CA'TA_TG
Cuts at: 387 2362 387
Size: 1975 4566
NgoMIV G'CCGG_C
Cuts at: 2989 2989
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NspI r_CATG'y
Cuts at: 1132 1229 3899 5444 1132
Size: 97 2670 1545 2229
Fragments arranged by size:
2670 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6541
PciI A'CATG_T
Cuts at: 5440 5440
Size: 6541
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2890 1108
Size: 1782 4759
PfoI T'CCnGG_A
Cuts at: 3408 3408
Size: 6541
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2567 2599 4610 4642 6123 6155 2567
Size: 32 2011 32 1481 32 2953
Fragments arranged by size:
2953 2011 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3004 3820 3004
Size: 816 5725
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6541
PsiI TTA'TAA
Cuts at: 4111 4111
Size: 6541
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3282 3314 3282
Size: 32 6509
PstI C_TGCA'G
Cuts at: 830 3184 3893 830
Size: 2354 709 3478
Fragments arranged by size:
3478 2354 709
PvuI CG_AT'CG
Cuts at: 2001 2629 2001
Size: 628 5913
PvuII CAG'CTG
Cuts at: 2234 2234
Size: 6541
SalI G'TCGA_C
Cuts at: 1936 3883 1936
Size: 1947 4594
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 4789 6501 4789
Size: 1712 4829
SfcI C'TryA_G
Cuts at: 826 1045 2753 3180 3600 3889 5024 5705 5896
Size: 219 1708 427 420 289 1135 681 191
Cuts at: 5896 826
Size: 1471
Fragments arranged by size:
1708 1471 1135 681 427 420 289 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 2567 3868 1872
Size: 695 1301 4545
Fragments arranged by size:
4545 1301 695
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3692 4645 5546 5808 820
Size: 197 926 1749 953 901 262 1553
Fragments arranged by size:
1749 1553 953 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4332 493
Size: 3839 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 3899 1132
Size: 2767 3774
SspI AAT'ATT
Cuts at: 5 52 4465 5
Size: 47 4413 2081
Fragments arranged by size:
4413 2081 47
StuI AGG'CCT
Cuts at: 1517 2486 1517
Size: 969 5572
StyI C'CwwG_G
Cuts at: 513 1065 2004 2284 2481 2910 3632 3980 513
Size: 552 939 280 197 429 722 348 3074
Fragments arranged by size:
3074 939 722 552 429 348 280 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2728 3363 4568 4585 4738 4923 5368
Size: 1255 1148 635 1205 17 153 185 445
Cuts at: 5368 325
Size: 1498
Fragments arranged by size:
1498 1255 1205 1148 635 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2202 2766 4787
Size: 274 80 33 51 1443 225 564 2021
Cuts at: 4787 6499 96
Size: 1712 138
Fragments arranged by size:
2021 1712 1443 564 274 225 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6501 6533 6501
Size: 32 6509
Tth111I GACn'n_nGTC
Cuts at: 1316 2866 1316
Size: 1550 4991
XbaI T'CTAG_A
Cuts at: 3877 3877
Size: 6541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2872 2872
Size: 6541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6541
XmaI C'CCGG_G
Cuts at: 1870 2565 3866 1870
Size: 695 1301 4545
Fragments arranged by size:
4545 1301 695
XmnI GAAnn'nnTTC
Cuts at: 4670 4670
Size: 6541
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4349 276
Size: 53 83 186 936 2815 2468
Fragments arranged by size:
2815 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PspOMI PspXI RsrII
SacI SacII SanDI SapI SexAI SfiI SfoI SrfI
SwaI