Restriction map for
pFN21AB0402
(Circular) (Six-base) MAPSORT of: pFN21AB0402.seq Check: 6932 from: 1 to: 6637
pFN21AB0402 6637 bp
With 182 enzymes: SgfI *
January 31, 2011 17:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3244 3244
Size: 6637
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4447 278
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3286 3980 1937
Size: 1349 694 4594
Fragments arranged by size:
4594 1349 694
Acc65I G'GTAC_C
Cuts at: 3076 3958 3076
Size: 882 5755
AclI AA'CG_TT
Cuts at: 1563 4764 5137 1563
Size: 3201 373 3063
Fragments arranged by size:
3201 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2147 2205 3084 3609 4700 6084 6352 1632
Size: 515 58 879 525 1091 1384 268 1917
Fragments arranged by size:
1917 1384 1091 879 525 515 268 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6440
AflIII A'CryG_T
Cuts at: 1203 2211 2984 5536 6513 1203
Size: 1008 773 2552 977 1327
Fragments arranged by size:
2552 1327 1008 977 773
AgeI A'CCGG_T
Cuts at: 2681 5468 2681
Size: 2787 3850
AhdI GACnn_n'nnGTC
Cuts at: 2659 5366 2659
Size: 2707 3930
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6637
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6637
AlwNI CAG_nnn'CTG
Cuts at: 2315 4380 5952 2315
Size: 2065 1572 3000
Fragments arranged by size:
3000 2065 1572
ApaI G_GGCC'C
Cuts at: 2405 2560 2405
Size: 155 6482
ApaLI G'TGCA_C
Cuts at: 4694 5850 4694
Size: 1156 5481
ApoI r'AATT_y
Cuts at: 1493 2357 2632 3093 3946 4172 4324 6433 1493
Size: 864 275 461 853 226 152 2109 1697
Fragments arranged by size:
2109 1697 864 853 461 275 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3879 5191 160
Size: 872 2847 1312 1606
Fragments arranged by size:
2847 1606 1312 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
AvaI C'yCGr_G
Cuts at: 1870 1943 3962 1870
Size: 73 2019 4545
Fragments arranged by size:
4545 2019 73
BamHI G'GATC_C
Cuts at: 3765 3967 4334 3765
Size: 202 367 6068
Fragments arranged by size:
6068 367 202
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 3232 3366 3958 5413
Size: 325 217 627 1289 156 134 592 1455
Cuts at: 5413 618
Size: 1842
Fragments arranged by size:
1842 1455 1289 627 592 325 217 156
134
BanII G_rGCy'C
Cuts at: 1391 2405 2465 2478 2560 3956 1391
Size: 1014 60 13 82 1396 4072
Fragments arranged by size:
4072 1396 1014 82 60 13
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3597 3632 928
Size: 971 1698 35 3933
Fragments arranged by size:
3933 1698 971 35
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4828 4862 1903
Size: 34 2891 34 3678
Fragments arranged by size:
3678 2891 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4529 5739 4529
Size: 1210 5427
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6637
BfrBI ATG'CAT
Cuts at: 2702 2702
Size: 6637
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5248 136
Size: 107 122 71 631 605 3576 1525
Fragments arranged by size:
3576 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6632 6632
Size: 6637
BlpI GC'TnA_GC
Cuts at: 4054 4054
Size: 6637
Bme1580I G_kGCm'C
Cuts at: 1790 2405 2560 3369 4698 5854 1790
Size: 615 155 809 1329 1156 2573
Fragments arranged by size:
2573 1329 1156 809 615 155
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6637
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2413 3131 3304
Size: 416 53 83 399 24 996 718 173
Cuts at: 3304 4368 5326 442
Size: 1064 958 1753
Fragments arranged by size:
1753 1064 996 958 718 416 399 173
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2020 2337 2376 2492 2634 2858 3135 5297
Size: 781 317 39 116 142 224 277 2162
Cuts at: 5297 1239
Size: 2579
Fragments arranged by size:
2579 2162 781 317 277 224 142 116
39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4762 5627 5925 4762
Size: 865 298 5474
Fragments arranged by size:
5474 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3020 5300 882
Size: 617 1521 2280 2219
Fragments arranged by size:
2280 2219 1521 617
BsaAI yAC'GTr
Cuts at: 493 1204 4428 493
Size: 711 3224 2702
Fragments arranged by size:
3224 2702 711
BsaBI GATnn'nnATC
Cuts at: 3447 6631 3447
Size: 3184 3453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3853 4444 4826 5461
Size: 53 83 186 936 2320 591 382 635
Cuts at: 5461 275
Size: 1451
Fragments arranged by size:
2320 1451 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2681 5070 5468 5742 5889 6300 2681
Size: 2389 398 274 147 411 3018
Fragments arranged by size:
3018 2389 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3818 3848 1178
Size: 30 165 30 2415 30 3967
Fragments arranged by size:
3967 2415 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2359 2855 1186
Size: 1173 496 4968
Fragments arranged by size:
4968 1173 496
BseYI C'CCAG_C
Cuts at: 5840 6336 5840
Size: 496 6141
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3222 3594 1163
Size: 573 1486 372 4206
Fragments arranged by size:
4206 1486 573 372
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4087 4848 5876 1540
Size: 275 186 2086 761 1028 2301
Fragments arranged by size:
2301 2086 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4698 4783 5854 4698
Size: 85 1071 5481
Fragments arranged by size:
5481 1071 85
BsmI GAATG_Cn'
Cuts at: 2327 2493 4148 4241 2327
Size: 166 1655 93 4723
Fragments arranged by size:
4723 1655 166 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 2465 2478 2560 3369 3956 4698
Size: 399 615 60 13 82 809 587 742
Cuts at: 4698 4783 5854 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 809 742 615 587 399 85
82 60 13
BspEI T'CCGG_A
Cuts at: 6300 6300
Size: 6637
BspHI T'CATG_A
Cuts at: 4526 6256 4526
Size: 1730 4907
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2387 3244 3992 844
Size: 1543 857 748 3489
Fragments arranged by size:
3489 1543 857 748
BsrBI CCG'CTC
Cuts at: 1394 1823 3253 4524 1394
Size: 429 1430 1271 3507
Fragments arranged by size:
3507 1430 1271 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2095 3708 5132 5306 65
Size: 1183 847 1613 1424 174 1396
Fragments arranged by size:
1613 1424 1396 1183 847 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2681 5281 5468 1700
Size: 110 871 2600 187 2869
Fragments arranged by size:
2869 2600 871 187 110
BsrGI T'GTAC_A
Cuts at: 96 2690 2773 96
Size: 2594 83 3960
Fragments arranged by size:
3960 2594 83
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6637
BssSI C'ACGA_G
Cuts at: 4697 5709 4697
Size: 1012 5625
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6637
BstBI TT'CG_AA
Cuts at: 2590 5441 2590
Size: 2851 3786
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6637
BstXI CCAn_nnnn'nTGG
Cuts at: 3362 3362
Size: 6637
BstYI r'GATC_y
Cuts at: 1972 3765 3967 4334 4719 4736 6188 6274 6286
Size: 1793 202 367 385 17 1452 86 12
Cuts at: 6286 6632 1972
Size: 346 1977
Fragments arranged by size:
1977 1793 1452 385 367 346 202 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2334 2364 3228 3331 3391 3481 3511
Size: 552 1269 30 864 103 60 90 30
Cuts at: 3511 513
Size: 3639
Fragments arranged by size:
3639 1269 864 552 103 90 60 30
30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5898
Fragments arranged by size:
5898 683 56
BtsI GCAGTG_nn'
Cuts at: 4153 4947 4967 4153
Size: 794 20 5823
Fragments arranged by size:
5823 794 20
ClaI AT'CG_AT
Cuts at: 1554 2709 1554
Size: 1155 5482
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6602
DraI TTT'AAA
Cuts at: 2107 3941 2107
Size: 1834 4803
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5644 809
Size: 507 4328 1802
Fragments arranged by size:
4328 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2970 3040 3328
Size: 54 1425 50 275 18 1140 70 288
Cuts at: 3328 3334 3388 3508 4084 4973 6602 8
Size: 6 54 120 576 889 1629 43
Fragments arranged by size:
1629 1425 1140 889 576 288 275 120
70 54 54 50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 4084 1537
Size: 275 2272 4090
Fragments arranged by size:
4090 2272 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2336 2537 3000 3243 3589 4567 1353
Size: 983 201 463 243 346 978 3423
Fragments arranged by size:
3423 983 978 463 346 243 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5224 5598 5744 387
Size: 1064 138 3635 374 146 1280
Fragments arranged by size:
3635 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2020 2147 2205 2337 2376 2492 2634
Size: 393 388 127 58 132 39 116 142
Cuts at: 2634 2858 3084 3135 3609 4700 5297 6084 6352
Size: 224 226 51 474 1091 597 787 268
Cuts at: 6352 1239
Size: 1524
Fragments arranged by size:
1524 1091 787 597 474 393 388 268
226 224 142 132 127 116 58 51
39
EcoO109I rG'GnC_Cy
Cuts at: 2401 2402 2401
Size: 1 6636
EcoRI G'AATT_C
Cuts at: 3946 4324 3946
Size: 378 6259
EcoRV GAT'ATC
Cuts at: 3775 3775
Size: 6637
FspI TGC'GCA
Cuts at: 5143 5143
Size: 6637
HaeII r_GCGC'y
Cuts at: 1678 5784 1678
Size: 4106 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2645 2677 2836 2868 3818
Size: 32 135 32 1073 32 159 32 950
Cuts at: 3818 3850 5278 5310 5352 5384 1373
Size: 32 1428 32 42 32 2626
Fragments arranged by size:
2626 1428 1073 950 159 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3012 3109 3116 3981 4227 669
Size: 988 281 1074 97 7 865 246 3079
Fragments arranged by size:
3079 1074 988 865 281 246 97 7
HindIII A'AGCT_T
Cuts at: 748 2867 748
Size: 2119 4518
HpaI GTT'AAC
Cuts at: 4227 4227
Size: 6637
KpnI G_GTAC'C
Cuts at: 3080 3962 3080
Size: 882 5755
MfeI C'AATT_G
Cuts at: 2604 4236 2604
Size: 1632 5005
MluI A'CGCG_T
Cuts at: 6513 6513
Size: 6637
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3539 3908 5751 5935 1305
Size: 2234 369 1843 184 2007
Fragments arranged by size:
2234 2007 1843 369 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2972 3042 3330 3336 3390
Size: 54 1425 343 1140 70 288 6 54
Cuts at: 3390 3510 10
Size: 120 3137
Fragments arranged by size:
3137 1425 1140 343 288 120 70 54
54 6
MslI CAynn'nnrTG
Cuts at: 518 1166 3227 3342 3348 3498 3504 3893 4595
Size: 648 2061 115 6 150 6 389 702
Cuts at: 4595 4954 5113 518
Size: 359 159 2042
Fragments arranged by size:
2061 2042 702 648 389 359 159 150
115 6 6
MspA1I CmG'CkG
Cuts at: 1606 4053 4730 5878 6123 1606
Size: 2447 677 1148 245 2120
Fragments arranged by size:
2447 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3228 3331 3391 3481 513
Size: 552 2163 103 60 90 3669
Fragments arranged by size:
3669 2163 552 103 90 60
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6637
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6637
NotI GC'GGCC_GC
Cuts at: 4084 4084
Size: 6637
NsiI A_TGCA'T
Cuts at: 2704 2704
Size: 6637
NspI r_CATG'y
Cuts at: 1132 1229 2988 3995 5540 1132
Size: 97 1759 1007 1545 2229
Fragments arranged by size:
2229 1759 1545 1007 97
PasI CC'CwG_GG
Cuts at: 1348 3081 1348
Size: 1733 4904
PciI A'CATG_T
Cuts at: 2984 5536 2984
Size: 2552 4085
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6637
PfoI T'CCnGG_A
Cuts at: 3847 3847
Size: 6637
PmeI GTTT'AAAC
Cuts at: 3941 3941
Size: 6637
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4706 4738 6219 6251 4706
Size: 32 1481 32 5092
Fragments arranged by size:
5092 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6637
PsiI TTA'TAA
Cuts at: 4207 4207
Size: 6637
PspOMI G'GGCC_C
Cuts at: 2401 2556 2401
Size: 155 6482
PstI C_TGCA'G
Cuts at: 830 3989 830
Size: 3159 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6637
SalI G'TCGA_C
Cuts at: 1936 3979 1936
Size: 2043 4594
SapI GCTCTTCn'nnn_
Cuts at: 3243 3589 3243
Size: 346 6291
SbfI CC_TGCA'GG
Cuts at: 3989 3989
Size: 6637
ScaI AGT'ACT
Cuts at: 2501 3598 4885 6597 2501
Size: 1097 1287 1712 2541
Fragments arranged by size:
2541 1712 1287 1097
SfcI C'TryA_G
Cuts at: 826 1045 3985 5120 5801 5992 826
Size: 219 2940 1135 681 191 1471
Fragments arranged by size:
2940 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6637
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6637
SmaI CCC'GGG
Cuts at: 1872 3964 1872
Size: 2092 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4741 5642 5904 820
Size: 197 926 2798 901 262 1553
Fragments arranged by size:
2798 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4428 493
Size: 3935 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6637
SphI G_CATG'C
Cuts at: 1132 3995 1132
Size: 2863 3774
SspI AAT'ATT
Cuts at: 5 52 2954 4561 5
Size: 47 2902 1607 2081
Fragments arranged by size:
2902 2081 1607 47
StuI AGG'CCT
Cuts at: 1517 3148 1517
Size: 1631 5006
StyI C'CwwG_G
Cuts at: 513 1065 2991 3228 3331 3355 3385 3391 3460
Size: 552 1926 237 103 24 30 6 69
Cuts at: 3460 3481 4076 513
Size: 21 595 3074
Fragments arranged by size:
3074 1926 595 552 237 103 69 30
24 21 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3029 3658 4664 4681 4834 5019 5464
Size: 1255 1449 629 1006 17 153 185 445
Cuts at: 5464 325
Size: 1498
Fragments arranged by size:
1498 1449 1255 1006 629 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2289 2499 2690
Size: 274 80 33 51 1443 312 210 191
Cuts at: 2690 2773 2929 3596 4883 6595 96
Size: 83 156 667 1287 1712 138
Fragments arranged by size:
1712 1443 1287 667 312 274 210 191
156 138 83 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6597 6629 6597
Size: 32 6605
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6637
XbaI T'CTAG_A
Cuts at: 3973 3973
Size: 6637
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2794 3377 2794
Size: 583 6054
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6637
XmaI C'CCGG_G
Cuts at: 1870 3962 1870
Size: 2092 4545
XmnI GAAnn'nnTTC
Cuts at: 2728 4766 2728
Size: 2038 4599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4445 276
Size: 53 83 186 936 2911 2468
Fragments arranged by size:
2911 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SexAI SfiI SfoI SrfI SwaI