Restriction map for
pFN21AB0356
(Circular) (Six-base) MAPSORT of: pFN21AB0356.seq Check: 241 from: 1 to: 7909
pFN21AB0356 7909 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5719 278
Size: 53 83 186 936 4183 2468
Fragments arranged by size:
4183 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2624 5252 1937
Size: 687 2628 4594
Fragments arranged by size:
4594 2628 687
Acc65I G'GTAC_C
Cuts at: 5230 5230
Size: 7909
AclI AA'CG_TT
Cuts at: 1563 2439 5208 6036 6409 1563
Size: 876 2769 828 373 3063
Fragments arranged by size:
3063 2769 876 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2691 3986 5972 7356 7624 1632
Size: 1059 1295 1986 1384 268 1917
Fragments arranged by size:
1986 1917 1384 1295 1059 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7712
AflIII A'CryG_T
Cuts at: 1203 4697 4855 6808 7785 1203
Size: 3494 158 1953 977 1327
Fragments arranged by size:
3494 1953 1327 977 158
AgeI A'CCGG_T
Cuts at: 6740 6740
Size: 7909
AhdI GACnn_n'nnGTC
Cuts at: 4369 6638 4369
Size: 2269 5640
AleI CACnn'nnGTG
Cuts at: 1166 3675 1166
Size: 2509 5400
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7909
AlwNI CAG_nnn'CTG
Cuts at: 5652 7224 5652
Size: 1572 6337
ApaLI G'TGCA_C
Cuts at: 3000 4608 5966 7122 3000
Size: 1608 1358 1156 3787
Fragments arranged by size:
3787 1608 1358 1156
ApoI r'AATT_y
Cuts at: 1493 2246 2384 3015 3693 3727 4561 4581 4835
Size: 753 138 631 678 34 834 20 254
Cuts at: 4835 4931 5218 5444 5596 7705 1493
Size: 96 287 226 152 2109 1697
Fragments arranged by size:
2109 1697 834 753 678 631 287 254
226 152 138 96 34 20
AseI AT'TA_AT
Cuts at: 160 1032 2129 6463 160
Size: 872 1097 4334 1606
Fragments arranged by size:
4334 1606 1097 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
AvaI C'yCGr_G
Cuts at: 1870 1943 3507 5234 1870
Size: 73 1564 1727 4545
Fragments arranged by size:
4545 1727 1564 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3297 3330 3297
Size: 33 7876
BamHI G'GATC_C
Cuts at: 5239 5606 5239
Size: 367 7542
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5230 6685 618
Size: 325 217 627 3443 1455 1842
Fragments arranged by size:
3443 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4909 5228 1391
Size: 3518 319 4072
Fragments arranged by size:
4072 3518 319
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6938
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 6100 6134 1903
Size: 34 50 34 4079 34 3678
Fragments arranged by size:
4079 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3514 4706 5801 7011 3514
Size: 1192 1095 1210 4412
Fragments arranged by size:
4412 1210 1192 1095
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7909
BfrBI ATG'CAT
Cuts at: 5201 5201
Size: 7909
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6520 136
Size: 107 122 71 631 605 4848 1525
Fragments arranged by size:
4848 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3063 7904 3063
Size: 4841 3068
BlpI GC'TnA_GC
Cuts at: 5326 5326
Size: 7909
Bme1580I G_kGCm'C
Cuts at: 1790 3004 3999 4612 5970 7126 1790
Size: 1214 995 613 1358 1156 2573
Fragments arranged by size:
2573 1358 1214 1156 995 613
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5640 6598 442
Size: 416 53 83 399 24 4223 958 1753
Fragments arranged by size:
4223 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7909
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3638
Size: 7909
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6569 1239
Size: 5330 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2211 6034 6899 7197 2211
Size: 3823 865 298 2923
Fragments arranged by size:
3823 2923 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6572 882
Size: 617 5073 2219
Fragments arranged by size:
5073 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3034 5700 493
Size: 711 1830 2666 2702
Fragments arranged by size:
2702 2666 1830 711
BsaBI GATnn'nnATC
Cuts at: 5142 7903 5142
Size: 2761 5148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5716 6098 6733 275
Size: 53 83 186 936 4183 382 635 1451
Fragments arranged by size:
4183 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6342 6740 7014 7161 7572 6342
Size: 398 274 147 411 6679
Fragments arranged by size:
6679 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7684
Fragments arranged by size:
7684 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3108 4254 1186
Size: 1922 1146 4841
Fragments arranged by size:
4841 1922 1146
BseYI C'CCAG_C
Cuts at: 7112 7608 7112
Size: 496 7413
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5359 6120 7148 1540
Size: 275 186 3358 761 1028 2301
Fragments arranged by size:
3358 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3004 4612 5970 6055 7126 3004
Size: 1608 1358 85 1071 3787
Fragments arranged by size:
3787 1608 1358 1071 85
BsmI GAATG_Cn'
Cuts at: 2114 4919 5037 5420 5513 2114
Size: 2805 118 383 93 4510
Fragments arranged by size:
4510 2805 383 118 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3004 3999 4612 4909 5228 5970 6055
Size: 399 1214 995 613 297 319 742 85
Cuts at: 6055 7126 1391
Size: 1071 2174
Fragments arranged by size:
2174 1214 1071 995 742 613 399 319
297 85
BspEI T'CCGG_A
Cuts at: 7572 7572
Size: 7909
BspHI T'CATG_A
Cuts at: 5121 5798 7528 5121
Size: 677 1730 5502
Fragments arranged by size:
5502 1730 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5264 844
Size: 4420 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5796 1394
Size: 429 3973 3507
Fragments arranged by size:
3973 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2499 4134 5058 6404 6578 65
Size: 1183 1251 1635 924 1346 174 1396
Fragments arranged by size:
1635 1396 1346 1251 1183 924 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6553 6740 1700
Size: 110 4743 187 2869
Fragments arranged by size:
4743 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7909
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7909
BssSI C'ACGA_G
Cuts at: 3195 3612 5969 6981 3195
Size: 417 2357 1012 4123
Fragments arranged by size:
4123 2357 1012 417
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4435 794
Size: 3641 4268
BstBI TT'CG_AA
Cuts at: 3073 4498 5071 6713 3073
Size: 1425 573 1642 4269
Fragments arranged by size:
4269 1642 1425 573
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7909
BstXI CCAn_nnnn'nTGG
Cuts at: 3273 4055 3273
Size: 782 7127
BstYI r'GATC_y
Cuts at: 1972 2337 2401 2458 3063 3616 3826 5175 5239
Size: 365 64 57 605 553 210 1349 64
Cuts at: 5239 5606 5991 6008 7460 7546 7558 7904 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1349 605 553 385 367 365
346 210 86 64 64 57 17 12
BstZ17I GTA'TAC
Cuts at: 2625 2625
Size: 7909
Bsu36I CC'TnA_GG
Cuts at: 4295 4295
Size: 7909
BtgI C'CryG_G
Cuts at: 513 1065 3088 513
Size: 552 2023 5334
Fragments arranged by size:
5334 2023 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7170
Fragments arranged by size:
7170 683 56
BtsI GCAGTG_nn'
Cuts at: 3454 4610 5425 6219 6239 3454
Size: 1156 815 794 20 5124
Fragments arranged by size:
5124 1156 815 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7909
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4326 4361 529
Size: 35 3762 35 4077
Fragments arranged by size:
4077 3762 35 35
DraI TTT'AAA
Cuts at: 2155 2301 2760 4017 4408 5213 2155
Size: 146 459 1257 391 805 4851
Fragments arranged by size:
4851 1257 805 459 391 146
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6916 809
Size: 507 5600 1802
Fragments arranged by size:
5600 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3341 5356 6245
Size: 54 1425 50 275 18 1511 2015 889
Cuts at: 6245 7874 8
Size: 1629 43
Fragments arranged by size:
2015 1629 1511 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5356 1537
Size: 275 3544 4090
Fragments arranged by size:
4090 3544 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3477 4299 5000 5839 1353
Size: 2124 822 701 839 3423
Fragments arranged by size:
3423 2124 839 822 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6496 6870 7016 387
Size: 1064 138 4907 374 146 1280
Fragments arranged by size:
4907 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2691 3986 5972 6569 7356 7624 1239
Size: 393 1059 1295 1986 597 787 268 1524
Fragments arranged by size:
1986 1524 1295 1059 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2117 2117
Size: 7909
EcoO109I rG'GnC_Cy
Cuts at: 2467 3916 2467
Size: 1449 6460
EcoRI G'AATT_C
Cuts at: 3727 5218 5596 3727
Size: 1491 378 6040
Fragments arranged by size:
6040 1491 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2897 3150 2897
Size: 253 7656
FspI TGC'GCA
Cuts at: 6415 6415
Size: 7909
HaeII r_GCGC'y
Cuts at: 1678 7056 1678
Size: 5378 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2329 2361 3608 3640 4355
Size: 32 135 32 757 32 1247 32 715
Cuts at: 4355 4387 6550 6582 6624 6656 1373
Size: 32 2163 32 42 32 2626
Fragments arranged by size:
2626 2163 1247 757 715 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5253 5499 669
Size: 988 281 3315 246 3079
Fragments arranged by size:
3315 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7909
HpaI GTT'AAC
Cuts at: 5499 5499
Size: 7909
KpnI G_GTAC'C
Cuts at: 5234 5234
Size: 7909
MfeI C'AATT_G
Cuts at: 3493 5508 3493
Size: 2015 5894
MluI A'CGCG_T
Cuts at: 7785 7785
Size: 7909
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3408 7023 7207 1305
Size: 2103 3615 184 2007
Fragments arranged by size:
3615 2103 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3343 10
Size: 54 1425 343 1511 4576
Fragments arranged by size:
4576 1511 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2375 2646 3271 3675 4574 4702 4769
Size: 648 1209 271 625 404 899 128 67
Cuts at: 4769 5148 5867 6226 6385 518
Size: 379 719 359 159 2042
Fragments arranged by size:
2042 1209 899 719 648 625 404 379
359 271 159 128 67
MspA1I CmG'CkG
Cuts at: 1606 5325 6002 7150 7395 1606
Size: 3719 677 1148 245 2120
Fragments arranged by size:
3719 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3088 513
Size: 552 2023 5334
Fragments arranged by size:
5334 2023 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7909
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7909
NotI GC'GGCC_GC
Cuts at: 5356 5356
Size: 7909
NsiI A_TGCA'T
Cuts at: 5203 5203
Size: 7909
NspI r_CATG'y
Cuts at: 1132 1229 2374 3491 4089 4988 5201 5267 6812
Size: 97 1145 1117 598 899 213 66 1545
Cuts at: 6812 1132
Size: 2229
Fragments arranged by size:
2229 1545 1145 1117 899 598 213 97
66
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7909
PciI A'CATG_T
Cuts at: 6808 6808
Size: 7909
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7909
PmeI GTTT'AAAC
Cuts at: 5213 5213
Size: 7909
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5978 6010 7491 7523 5978
Size: 32 1481 32 6364
Fragments arranged by size:
6364 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 3916 2467
Size: 1449 6460
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7909
PsiI TTA'TAA
Cuts at: 2993 4845 5479 2993
Size: 1852 634 5423
Fragments arranged by size:
5423 1852 634
PstI C_TGCA'G
Cuts at: 830 4617 5261 830
Size: 3787 644 3478
Fragments arranged by size:
3787 3478 644
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7909
SalI G'TCGA_C
Cuts at: 1936 5251 1936
Size: 3315 4594
SapI GCTCTTCn'nnn_
Cuts at: 3477 3477
Size: 7909
SbfI CC_TGCA'GG
Cuts at: 5261 5261
Size: 7909
ScaI AGT'ACT
Cuts at: 6157 7869 6157
Size: 1712 6197
SfcI C'TryA_G
Cuts at: 826 1045 4247 4613 5257 6392 7073 7264 826
Size: 219 3202 366 644 1135 681 191 1471
Fragments arranged by size:
3202 1471 1135 681 644 366 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7909
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7909
SmaI CCC'GGG
Cuts at: 1872 5236 1872
Size: 3364 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2226 6013 6914 7176 820
Size: 197 926 283 3787 901 262 1553
Fragments arranged by size:
3787 1553 926 901 283 262 197
SnaBI TAC'GTA
Cuts at: 493 5700 493
Size: 5207 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7909
SphI G_CATG'C
Cuts at: 1132 2374 3491 4988 5201 5267 1132
Size: 1242 1117 1497 213 66 3774
Fragments arranged by size:
3774 1497 1242 1117 213 66
SspI AAT'ATT
Cuts at: 5 52 2431 3046 3869 3935 5833 5
Size: 47 2379 615 823 66 1898 2081
Fragments arranged by size:
2379 2081 1898 823 615 66 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7909
StyI C'CwwG_G
Cuts at: 513 1065 3088 5348 513
Size: 552 2023 2260 3074
Fragments arranged by size:
3074 2260 2023 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5936 5953 6106 6291 6736 325
Size: 1255 4356 17 153 185 445 1498
Fragments arranged by size:
4356 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2410 3910 6155
Size: 274 80 33 51 1443 433 1500 2245
Cuts at: 6155 7867 96
Size: 1712 138
Fragments arranged by size:
2245 1712 1500 1443 433 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2082 2114 7869 7901 2082
Size: 32 5755 32 2090
Fragments arranged by size:
5755 2090 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7909
XbaI T'CTAG_A
Cuts at: 3731 5245 3731
Size: 1514 6395
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7909
XmaI C'CCGG_G
Cuts at: 1870 5234 1870
Size: 3364 4545
XmnI GAAnn'nnTTC
Cuts at: 6038 6038
Size: 7909
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5717 276
Size: 53 83 186 936 4183 2468
Fragments arranged by size:
4183 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI