Restriction map for
pFN21AB0207
(Circular) (Six-base) MAPSORT of: pFN21AB0207.seq Check: 5477 from: 1 to: 7774
pFN21AB0207 7774 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4765 4765
Size: 7774
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5058 5584 278
Size: 53 83 186 936 3522 526 2468
Fragments arranged by size:
3522 2468 936 526 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5117 1937
Size: 3180 4594
Acc65I G'GTAC_C
Cuts at: 5095 5095
Size: 7774
AclI AA'CG_TT
Cuts at: 1563 5901 6274 1563
Size: 4338 373 3063
Fragments arranged by size:
4338 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3219 4019 4850 5837 7221 7489 1632
Size: 1587 800 831 987 1384 268 1917
Fragments arranged by size:
1917 1587 1384 987 831 800 268
AfeI AGC'GCT
Cuts at: 2834 2834
Size: 7774
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7577
AflIII A'CryG_T
Cuts at: 1203 3459 6673 7650 1203
Size: 2256 3214 977 1327
Fragments arranged by size:
3214 2256 1327 977
AgeI A'CCGG_T
Cuts at: 6605 6605
Size: 7774
AhdI GACnn_n'nnGTC
Cuts at: 6503 6503
Size: 7774
AleI CACnn'nnGTG
Cuts at: 1166 3392 1166
Size: 2226 5548
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3252 4349 1184
Size: 2068 1097 4609
Fragments arranged by size:
4609 2068 1097
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2657 2689 2657
Size: 32 7742
AlwNI CAG_nnn'CTG
Cuts at: 2341 4543 4979 5517 7089 2341
Size: 2202 436 538 1572 3026
Fragments arranged by size:
3026 2202 1572 538 436
ApaLI G'TGCA_C
Cuts at: 3317 5831 6987 3317
Size: 2514 1156 4104
Fragments arranged by size:
4104 2514 1156
ApoI r'AATT_y
Cuts at: 1493 3678 5083 5309 5461 7570 1493
Size: 2185 1405 226 152 2109 1697
Fragments arranged by size:
2185 2109 1697 1405 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6328 160
Size: 872 5296 1606
Fragments arranged by size:
5296 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
AvaI C'yCGr_G
Cuts at: 1870 1943 3399 3443 4294 4601 4743 5099 1870
Size: 73 1456 44 851 307 142 356 4545
Fragments arranged by size:
4545 1456 851 356 307 142 73 44
BamHI G'GATC_C
Cuts at: 2703 5104 5471 2703
Size: 2401 367 5006
Fragments arranged by size:
5006 2401 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2427 3311 4869 5095 6550
Size: 325 217 627 640 884 1558 226 1455
Cuts at: 6550 618
Size: 1842
Fragments arranged by size:
1842 1558 1455 884 640 627 325 226
217
BanII G_rGCy'C
Cuts at: 1391 2148 2266 3035 3561 3967 5093 1391
Size: 757 118 769 526 406 1126 4072
Fragments arranged by size:
4072 1126 769 757 526 406 118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2551 3929 5009 5050 928
Size: 971 652 1378 1080 41 3652
Fragments arranged by size:
3652 1378 1080 971 652 41
BbvCI CC'TCA_GC
Cuts at: 3281 3281
Size: 7774
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5965 5999 1903
Size: 34 4028 34 3678
Fragments arranged by size:
4028 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5666 6876 5666
Size: 1210 6564
BclI T'GATC_A
Cuts at: 1548 2919 1548
Size: 1371 6403
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6385 136
Size: 107 122 71 631 605 4713 1525
Fragments arranged by size:
4713 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2988 3696 3719 3735 7769 2988
Size: 708 23 16 4034 2993
Fragments arranged by size:
4034 2993 708 23 16
BlpI GC'TnA_GC
Cuts at: 2142 3627 3908 4022 4857 5191 2142
Size: 1485 281 114 835 334 4725
Fragments arranged by size:
4725 1485 835 334 281 114
Bme1580I G_kGCm'C
Cuts at: 1790 2276 2430 2490 3321 4049 5835 6991 1790
Size: 486 154 60 831 728 1786 1156 2573
Fragments arranged by size:
2573 1786 1156 831 728 486 154 60
BmgBI CAC'GTC
Cuts at: 1318 3394 3505 4036 1318
Size: 2076 111 531 5056
Fragments arranged by size:
5056 2076 531 111
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3970 4261 5505
Size: 416 53 83 399 24 2553 291 1244
Cuts at: 5505 6463 442
Size: 958 1753
Fragments arranged by size:
2553 1753 1244 958 416 399 291 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7774
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2654 2708 3035 4968 6434 1239
Size: 1415 54 327 1933 1466 2579
Fragments arranged by size:
2579 1933 1466 1415 327 54
Bpu10I CC'TnA_GC
Cuts at: 2300 3281 2300
Size: 981 6793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2858 3155 5899 6764 7062 2858
Size: 297 2744 865 298 3570
Fragments arranged by size:
3570 2744 865 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2714 3263 6437 882
Size: 617 1215 549 3174 2219
Fragments arranged by size:
3174 2219 1215 617 549
BsaAI yAC'GTr
Cuts at: 493 1204 2917 3322 3427 3460 4525 4909 5565
Size: 711 1713 405 105 33 1065 384 656
Cuts at: 5565 493
Size: 2702
Fragments arranged by size:
2702 1713 1065 711 656 405 384 105
33
BsaBI GATnn'nnATC
Cuts at: 2469 7768 2469
Size: 5299 2475
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4534 5055 5581 5963
Size: 53 83 186 936 3001 521 526 382
Cuts at: 5963 6598 275
Size: 635 1451
Fragments arranged by size:
3001 1451 936 635 526 521 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 3035 3948 4554 6207 6605 6879 7026 7437 3035
Size: 913 606 1653 398 274 147 411 3372
Fragments arranged by size:
3372 1653 913 606 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2171 2201 2657 2687 1178
Size: 30 165 30 768 30 456 30 6265
Fragments arranged by size:
6265 768 456 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2195 2470 2765 2813 3364 3499 3686 4892
Size: 1009 275 295 48 551 135 187 1206
Cuts at: 4892 1186
Size: 4068
Fragments arranged by size:
4068 1206 1009 551 295 275 187 135
48
BseYI C'CCAG_C
Cuts at: 4180 4192 4972 6977 7473 4180
Size: 12 780 2005 496 4481
Fragments arranged by size:
4481 2005 780 496 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2112 2414 4699 4859 1163
Size: 573 376 302 2285 160 4078
Fragments arranged by size:
4078 2285 573 376 302 160
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3477 4966 5224 5985 7013 1540
Size: 275 186 1476 1489 258 761 1028 2301
Fragments arranged by size:
2301 1489 1476 1028 761 275 258 186
BsiHKAI G_wGCw'C
Cuts at: 3321 3491 4074 4884 5835 5920 6991 3321
Size: 170 583 810 951 85 1071 4104
Fragments arranged by size:
4104 1071 951 810 583 170 85
BsmI GAATG_Cn'
Cuts at: 2260 3415 5285 5378 2260
Size: 1155 1870 93 4656
Fragments arranged by size:
4656 1870 1155 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2266 2276 2430 2490 3035 3321
Size: 399 358 118 10 154 60 545 286
Cuts at: 3321 3491 3561 3967 4049 4074 4884 5093 5835
Size: 170 70 406 82 25 810 209 742
Cuts at: 5835 5920 6991 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 810 742 545 406 399 358
286 209 170 154 118 85 82 70
60 25 10
BspEI T'CCGG_A
Cuts at: 3035 4554 7437 3035
Size: 1519 2883 3372
Fragments arranged by size:
3372 2883 1519
BspHI T'CATG_A
Cuts at: 5663 7393 5663
Size: 1730 6044
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3297 4109 4765 5129 844
Size: 2453 812 656 364 3489
Fragments arranged by size:
3489 2453 812 656 364
BsrBI CCG'CTC
Cuts at: 1394 1823 3372 3577 4026 4042 4393 5661 1394
Size: 429 1549 205 449 16 351 1268 3507
Fragments arranged by size:
3507 1549 1268 449 429 351 205 16
BsrDI GCAATG_nn'
Cuts at: 65 1248 6269 6443 65
Size: 1183 5021 174 1396
Fragments arranged by size:
5021 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3528 4718 4961 4991 6418 6605 1700
Size: 110 1718 1190 243 30 1427 187 2869
Fragments arranged by size:
2869 1718 1427 1190 243 187 110 30
BsrGI T'GTAC_A
Cuts at: 96 2218 3582 96
Size: 2122 1364 4288
Fragments arranged by size:
4288 2122 1364
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7774
BssSI C'ACGA_G
Cuts at: 2372 3927 5834 6846 2372
Size: 1555 1907 1012 3300
Fragments arranged by size:
3300 1907 1555 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2135 3813 794
Size: 1341 1678 4755
Fragments arranged by size:
4755 1678 1341
BstBI TT'CG_AA
Cuts at: 2168 6578 2168
Size: 4410 3364
BstEII G'GTnAC_C
Cuts at: 1184 3437 1184
Size: 2253 5521
BstXI CCAn_nnnn'nTGG
Cuts at: 2657 3394 2657
Size: 737 7037
BstYI r'GATC_y
Cuts at: 1972 2703 2988 3591 3696 3719 3735 3915 4539
Size: 731 285 603 105 23 16 180 624
Cuts at: 4539 4610 5104 5471 5856 5873 7325 7411 7423
Size: 71 494 367 385 17 1452 86 12
Cuts at: 7423 7769 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 731 624 603 494 385 367
346 285 180 105 86 71 23 17
16 12
BtgI C'CryG_G
Cuts at: 513 1065 3150 3822 4203 4345 513
Size: 552 2085 672 381 142 3942
Fragments arranged by size:
3942 2085 672 552 381 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7035
Fragments arranged by size:
7035 683 56
BtsI GCAGTG_nn'
Cuts at: 2274 2397 2699 5290 6084 6104 2274
Size: 123 302 2591 794 20 3944
Fragments arranged by size:
3944 2591 794 302 123 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7774
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2776 2811 4289 4324 529
Size: 35 2212 35 1478 35 3979
Fragments arranged by size:
3979 2212 1478 35 35 35
DraI TTT'AAA
Cuts at: 5078 5078
Size: 7774
DraIII CAC_nnn'GTG
Cuts at: 4069 4069
Size: 7774
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3509 4424 5054 6781 809
Size: 507 2193 915 630 1727 1802
Fragments arranged by size:
2193 1802 1727 915 630 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2769 3117 3250
Size: 54 1425 50 275 18 939 348 133
Cuts at: 3250 3474 3538 4027 4206 4779 4963 5221 6110
Size: 224 64 489 179 573 184 258 889
Cuts at: 6110 7739 8
Size: 1629 43
Fragments arranged by size:
1629 1425 939 889 573 489 348 275
258 224 184 179 133 64 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3474 4963 5221 1537
Size: 275 1662 1489 258 4090
Fragments arranged by size:
4090 1662 1489 275 258
EarI CTCTTCn'nnn_
Cuts at: 1353 4715 5704 1353
Size: 3362 989 3423
Fragments arranged by size:
3423 3362 989
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4369 6361 6735 6881 387
Size: 1064 138 2780 1992 374 146 1280
Fragments arranged by size:
2780 1992 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2654 2708 3035 3219 4019 4850 4968
Size: 393 1022 54 327 184 800 831 118
Cuts at: 4968 5837 6434 7221 7489 1239
Size: 869 597 787 268 1524
Fragments arranged by size:
1524 1022 869 831 800 787 597 393
327 268 184 118 54
EcoNI CCTnn'n_nnAGG
Cuts at: 2552 4001 4829 2552
Size: 1449 828 5497
Fragments arranged by size:
5497 1449 828
EcoO109I rG'GnC_Cy
Cuts at: 4135 4969 4135
Size: 834 6940
EcoRI G'AATT_C
Cuts at: 5083 5461 5083
Size: 378 7396
FspI TGC'GCA
Cuts at: 6280 6280
Size: 7774
HaeII r_GCGC'y
Cuts at: 1678 2836 5029 6921 1678
Size: 1158 2193 1892 2531
Fragments arranged by size:
2531 2193 1892 1158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2462 2486 2494 2518 2611
Size: 32 135 32 890 24 8 24 93
Cuts at: 2611 2643 2980 3012 3017 3049 3278 3310 4524
Size: 32 337 32 5 32 229 32 1214
Cuts at: 4524 4556 4595 4627 4787 4819 6415 6447 6489
Size: 32 39 32 160 32 1596 32 42
Cuts at: 6489 6521 1373
Size: 32 2626
Fragments arranged by size:
2626 1596 1214 890 337 229 160 135
93 42 39 32 32 32 32 32
32 32 32 32 32 32 24 24
8 5
HincII GTy'rAC
Cuts at: 669 1657 1938 3331 3655 4495 5118 5364 669
Size: 988 281 1393 324 840 623 246 3079
Fragments arranged by size:
3079 1393 988 840 623 324 281 246
HindIII A'AGCT_T
Cuts at: 748 3708 748
Size: 2960 4814
HpaI GTT'AAC
Cuts at: 5364 5364
Size: 7774
KpnI G_GTAC'C
Cuts at: 5099 5099
Size: 7774
MfeI C'AATT_G
Cuts at: 4480 5373 4480
Size: 893 6881
MluI A'CGCG_T
Cuts at: 7650 7650
Size: 7774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6888 7072 1305
Size: 5583 184 2007
Fragments arranged by size:
5583 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2771 3119 3252 4208 4781
Size: 54 1425 343 939 348 133 956 573
Cuts at: 4781 10
Size: 3003
Fragments arranged by size:
3003 1425 956 939 573 348 343 133
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2927 3234 3392 3458 4109 5732 6091
Size: 648 1761 307 158 66 651 1623 359
Cuts at: 6091 6250 518
Size: 159 2042
Fragments arranged by size:
2042 1761 1623 651 648 359 307 159
158 66
MspA1I CmG'CkG
Cuts at: 1606 2443 2492 2812 3023 3143 4651 4801 4976
Size: 837 49 320 211 120 1508 150 175
Cuts at: 4976 5190 5867 7015 7260 1606
Size: 214 677 1148 245 2120
Fragments arranged by size:
2120 1508 1148 837 677 320 245 214
211 175 150 120 49
NaeI GCC'GGC
Cuts at: 4963 4963
Size: 7774
NcoI C'CATG_G
Cuts at: 513 1065 4203 4345 513
Size: 552 3138 142 3942
Fragments arranged by size:
3942 3138 552 142
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7774
NgoMIV G'CCGG_C
Cuts at: 4961 4961
Size: 7774
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7774
NotI GC'GGCC_GC
Cuts at: 5221 5221
Size: 7774
NspI r_CATG'y
Cuts at: 1132 1229 2932 3064 3811 5132 6677 1132
Size: 97 1703 132 747 1321 1545 2229
Fragments arranged by size:
2229 1703 1545 1321 747 132 97
PasI CC'CwG_GG
Cuts at: 1348 2452 2726 1348
Size: 1104 274 6396
Fragments arranged by size:
6396 1104 274
PciI A'CATG_T
Cuts at: 6673 6673
Size: 7774
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4036 4087 4351 4667 1108
Size: 2928 51 264 316 4215
Fragments arranged by size:
4215 2928 316 264 51
PfoI T'CCnGG_A
Cuts at: 4633 5031 4633
Size: 398 7376
PmeI GTTT'AAAC
Cuts at: 5078 5078
Size: 7774
PmlI CAC'GTG
Cuts at: 3322 3427 4525 3322
Size: 105 1098 6571
Fragments arranged by size:
6571 1098 105
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2657 2689 3548 3580 5843 5875 7356 7388 2657
Size: 32 859 32 2263 32 1481 32 3043
Fragments arranged by size:
3043 2263 1481 859 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4969 4969
Size: 7774
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7774
PsiI TTA'TAA
Cuts at: 5344 5344
Size: 7774
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4880 4912 4880
Size: 32 7742
PstI C_TGCA'G
Cuts at: 830 4678 5025 5126 830
Size: 3848 347 101 3478
Fragments arranged by size:
3848 3478 347 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7774
PvuII CAG'CTG
Cuts at: 2443 3023 4801 2443
Size: 580 1778 5416
Fragments arranged by size:
5416 1778 580
SalI G'TCGA_C
Cuts at: 1936 5116 1936
Size: 3180 4594
SanDI GG'GwC_CC
Cuts at: 4969 4969
Size: 7774
SbfI CC_TGCA'GG
Cuts at: 5126 5126
Size: 7774
ScaI AGT'ACT
Cuts at: 6022 7734 6022
Size: 1712 6062
SexAI A'CCwGG_T
Cuts at: 2789 2789
Size: 7774
SfcI C'TryA_G
Cuts at: 826 1045 2807 4674 5021 5122 6257 6938 7129
Size: 219 1762 1867 347 101 1135 681 191
Cuts at: 7129 826
Size: 1471
Fragments arranged by size:
1867 1762 1471 1135 681 347 219 191
101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7774
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7774
SmaI CCC'GGG
Cuts at: 1872 4296 4745 5101 1872
Size: 2424 449 356 4545
Fragments arranged by size:
4545 2424 449 356
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2873 3134 5878 6779 7041 820
Size: 197 926 930 261 2744 901 262 1553
Fragments arranged by size:
2744 1553 930 926 901 262 261 197
SnaBI TAC'GTA
Cuts at: 493 5565 493
Size: 5072 2702
SpeI A'CTAG_T
Cuts at: 152 2162 152
Size: 2010 5764
SphI G_CATG'C
Cuts at: 1132 2932 5132 1132
Size: 1800 2200 3774
Fragments arranged by size:
3774 2200 1800
SspI AAT'ATT
Cuts at: 5 52 5698 5
Size: 47 5646 2081
Fragments arranged by size:
5646 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7774
StyI C'CwwG_G
Cuts at: 513 1065 3111 4203 4345 4891 5213 513
Size: 552 2046 1092 142 546 322 3074
Fragments arranged by size:
3074 2046 1092 552 546 322 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4300 5801 5818 5971 6156 6601 325
Size: 1255 2720 1501 17 153 185 445 1498
Fragments arranged by size:
2720 1501 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2187 2218 3582
Size: 274 80 33 51 1443 210 31 1364
Cuts at: 3582 3975 6020 7732 96
Size: 393 2045 1712 138
Fragments arranged by size:
2045 1712 1443 1364 393 274 210 138
80 51 33 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3067 3099 7734 7766 3067
Size: 32 4635 32 3075
Fragments arranged by size:
4635 3075 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2555 1316
Size: 1239 6535
XbaI T'CTAG_A
Cuts at: 2842 5110 2842
Size: 2268 5506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4858 4858
Size: 7774
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7774
XmaI C'CCGG_G
Cuts at: 1870 4294 4743 5099 1870
Size: 2424 449 356 4545
Fragments arranged by size:
4545 2424 449 356
XmnI GAAnn'nnTTC
Cuts at: 5903 5903
Size: 7774
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5056 5582 276
Size: 53 83 186 936 3522 526 2468
Fragments arranged by size:
3522 2468 936 526 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PspOMI PspXI RsrII
SacI SacII SapI SfiI SfoI SrfI SwaI