Restriction map for
pFN21AB0179
(Circular) (Six-base) MAPSORT of: pFN21AB0179.seq Check: 3852 from: 1 to: 8365
pFN21AB0179 8365 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5386 5386
Size: 8365
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6175 278
Size: 53 83 186 936 4639 2468
Fragments arranged by size:
4639 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5708 1937
Size: 3771 4594
Acc65I G'GTAC_C
Cuts at: 5686 5686
Size: 8365
AclI AA'CG_TT
Cuts at: 1563 6492 6865 1563
Size: 4929 373 3063
Fragments arranged by size:
4929 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2369 3162 3734 5139 6428 7812 8080 1632
Size: 737 793 572 1405 1289 1384 268 1917
Fragments arranged by size:
1917 1405 1384 1289 793 737 572 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8168
AflIII A'CryG_T
Cuts at: 1203 7264 8241 1203
Size: 6061 977 1327
Fragments arranged by size:
6061 1327 977
AgeI A'CCGG_T
Cuts at: 7196 7196
Size: 8365
AhdI GACnn_n'nnGTC
Cuts at: 7094 7094
Size: 8365
AleI CACnn'nnGTG
Cuts at: 1166 4088 4751 1166
Size: 2922 663 4780
Fragments arranged by size:
4780 2922 663
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2511 1184
Size: 1327 7038
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4398 4430 4398
Size: 32 8333
AlwNI CAG_nnn'CTG
Cuts at: 3754 3779 3808 6108 7680 3754
Size: 25 29 2300 1572 4439
Fragments arranged by size:
4439 2300 1572 29 25
ApaI G_GGCC'C
Cuts at: 4903 5230 4903
Size: 327 8038
ApaLI G'TGCA_C
Cuts at: 2888 6422 7578 2888
Size: 3534 1156 3675
Fragments arranged by size:
3675 3534 1156
ApoI r'AATT_y
Cuts at: 1493 2156 2231 3248 5105 5674 5900 6052 8161
Size: 663 75 1017 1857 569 226 152 2109
Cuts at: 8161 1493
Size: 1697
Fragments arranged by size:
2109 1857 1697 1017 663 569 226 152
75
AseI AT'TA_AT
Cuts at: 160 1032 6919 160
Size: 872 5887 1606
Fragments arranged by size:
5887 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8365
AvaI C'yCGr_G
Cuts at: 1870 1943 2078 2640 5646 5690 1870
Size: 73 135 562 3006 44 4545
Fragments arranged by size:
4545 3006 562 135 73 44
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2066 2099 5013 5046 2066
Size: 33 2914 33 5385
Fragments arranged by size:
5385 2914 33 33
BamHI G'GATC_C
Cuts at: 5695 6062 5695
Size: 367 7998
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5686 7141 618
Size: 325 217 627 3899 1455 1842
Fragments arranged by size:
3899 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2790 2904 3386 4903 5230 5684 1391
Size: 1399 114 482 1517 327 454 4072
Fragments arranged by size:
4072 1517 1399 482 454 327 114
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4330 5047 928
Size: 971 2431 717 4246
Fragments arranged by size:
4246 2431 971 717
BbvCI CC'TCA_GC
Cuts at: 4153 4153
Size: 8365
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3858 3892 4518 4552 6556 6590 1903
Size: 34 1921 34 626 34 2004 34 3678
Fragments arranged by size:
3678 2004 1921 626 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5041 6257 7467 5041
Size: 1216 1210 5939
Fragments arranged by size:
5939 1216 1210
BclI T'GATC_A
Cuts at: 1548 2163 4866 1548
Size: 615 2703 5047
Fragments arranged by size:
5047 2703 615
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6976 136
Size: 107 122 71 631 605 5304 1525
Fragments arranged by size:
5304 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3037 8360 3037
Size: 5323 3042
BlpI GC'TnA_GC
Cuts at: 5570 5782 5570
Size: 212 8153
Bme1580I G_kGCm'C
Cuts at: 1790 2892 3360 4903 5230 5425 6426 7582 1790
Size: 1102 468 1543 327 195 1001 1156 2573
Fragments arranged by size:
2573 1543 1156 1102 1001 468 327 195
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8365
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2357 2994 3538
Size: 416 53 83 399 24 940 637 544
Cuts at: 3538 3796 4430 4449 4904 6096 7054 442
Size: 258 634 19 455 1192 958 1753
Fragments arranged by size:
1753 1192 958 940 637 634 544 455
416 399 258 83 53 24 19
BmtI G_CTAG'C
Cuts at: 1056 5144 1056
Size: 4088 4277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3106 3172 5661 3106
Size: 66 2489 5810
Fragments arranged by size:
5810 2489 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2271 2763 3051 4979 5656 7025 1239
Size: 1032 492 288 1928 677 1369 2579
Fragments arranged by size:
2579 1928 1369 1032 677 492 288
Bpu10I CC'TnA_GC
Cuts at: 3002 3916 4153 4650 3002
Size: 914 237 497 6717
Fragments arranged by size:
6717 914 497 237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3335 3701 4784 6490 7355 7653 3335
Size: 366 1083 1706 865 298 4047
Fragments arranged by size:
4047 1706 1083 865 366 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2586 3068 3080 5557 7028 882
Size: 617 1087 482 12 2477 1471 2219
Fragments arranged by size:
2477 2219 1471 1087 617 482 12
BsaAI yAC'GTr
Cuts at: 493 1204 6156 493
Size: 711 4952 2702
Fragments arranged by size:
4952 2702 711
BsaBI GATnn'nnATC
Cuts at: 3018 3674 8359 3018
Size: 656 4685 3024
Fragments arranged by size:
4685 3024 656
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4634 6172 6554 7189
Size: 53 83 186 936 3101 1538 382 635
Cuts at: 7189 275
Size: 1451
Fragments arranged by size:
3101 1538 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2377 2706 5376 6798 7196 7470 7617 8028 2377
Size: 329 2670 1422 398 274 147 411 2714
Fragments arranged by size:
2714 2670 1422 411 398 329 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2671 2677 2701 2707 4871
Size: 30 165 30 1268 6 24 6 2164
Cuts at: 4871 4901 5159 5189 1178
Size: 30 258 30 4354
Fragments arranged by size:
4354 2164 1268 258 165 30 30 30
30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2766 3098 3914 1186
Size: 1580 332 816 5637
Fragments arranged by size:
5637 1580 816 332
BseYI C'CCAG_C
Cuts at: 2465 3480 3852 4483 4676 5536 7568 8064 2465
Size: 1015 372 631 193 860 2032 496 2766
Fragments arranged by size:
2766 2032 1015 860 631 496 372 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2247 2870 3940 5066 5333 1163
Size: 573 511 623 1070 1126 267 4195
Fragments arranged by size:
4195 1126 1070 623 573 511 267
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5815 6576 7604 1540
Size: 275 186 3814 761 1028 2301
Fragments arranged by size:
3814 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2892 2904 3139 4095 5472 6426 6511 7582 2892
Size: 12 235 956 1377 954 85 1071 3675
Fragments arranged by size:
3675 1377 1071 956 954 235 85 12
BsmI GAATG_Cn'
Cuts at: 3276 5876 5969 3276
Size: 2600 93 5672
Fragments arranged by size:
5672 2600 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2270 2270
Size: 8365
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2790 2892 2904 3139 3360 3386 4095
Size: 399 1000 102 12 235 221 26 709
Cuts at: 4095 4903 5230 5425 5472 5684 6426 6511 7582
Size: 808 327 195 47 212 742 85 1071
Cuts at: 7582 1391
Size: 2174
Fragments arranged by size:
2174 1071 1000 808 742 709 399 327
235 221 212 195 102 85 47 26
12
BspEI T'CCGG_A
Cuts at: 8028 8028
Size: 8365
BspHI T'CATG_A
Cuts at: 6254 7984 6254
Size: 1730 6635
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5386 5720 844
Size: 4542 334 3489
Fragments arranged by size:
4542 3489 334
BsrBI CCG'CTC
Cuts at: 1394 1823 2619 2734 6252 1394
Size: 429 796 115 3518 3507
Fragments arranged by size:
3518 3507 796 429 115
BsrDI GCAATG_nn'
Cuts at: 65 1248 3438 6860 7034 65
Size: 1183 2190 3422 174 1396
Fragments arranged by size:
3422 2190 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7009 7196 1700
Size: 110 5199 187 2869
Fragments arranged by size:
5199 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4245 4710 4887 5148 5607 96
Size: 4149 465 177 261 459 2854
Fragments arranged by size:
4149 2854 465 459 261 177
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8365
BssSI C'ACGA_G
Cuts at: 4625 6425 7437 4625
Size: 1800 1012 5553
Fragments arranged by size:
5553 1800 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8365
BstBI TT'CG_AA
Cuts at: 7169 7169
Size: 8365
BstEII G'GTnAC_C
Cuts at: 1184 5372 1184
Size: 4188 4177
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 3201 3865 2011
Size: 1190 664 6511
Fragments arranged by size:
6511 1190 664
BstYI r'GATC_y
Cuts at: 1972 2311 3037 3750 3776 4377 5695 6062 6447
Size: 339 726 713 26 601 1318 367 385
Cuts at: 6447 6464 7916 8002 8014 8360 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1318 726 713 601 385 367
346 339 86 26 17 12
Bsu36I CC'TnA_GG
Cuts at: 3285 3285
Size: 8365
BtgI C'CryG_G
Cuts at: 513 1065 2004 2022 2058 3510 4806 4875 5183
Size: 552 939 18 36 1452 1296 69 308
Cuts at: 5183 513
Size: 3695
Fragments arranged by size:
3695 1452 1296 939 552 308 69 36
18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4521 487
Size: 683 56 3295 4331
Fragments arranged by size:
4331 3295 683 56
BtsI GCAGTG_nn'
Cuts at: 4176 5014 5335 5881 6675 6695 4176
Size: 838 321 546 794 20 5846
Fragments arranged by size:
5846 838 794 546 321 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8365
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8330
DraI TTT'AAA
Cuts at: 5669 5669
Size: 8365
DraIII CAC_nnn'GTG
Cuts at: 4325 4325
Size: 8365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3670 7372 809
Size: 507 2354 3702 1802
Fragments arranged by size:
3702 2354 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2922 3450 3882
Size: 54 1425 50 275 18 1092 528 432
Cuts at: 3882 5812 6701 8330 8
Size: 1930 889 1629 43
Fragments arranged by size:
1930 1629 1425 1092 889 528 432 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5812 1537
Size: 275 4000 4090
Fragments arranged by size:
4090 4000 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2363 2778 3261 3330 3856 4455 5432 6295
Size: 1010 415 483 69 526 599 977 863
Cuts at: 6295 1353
Size: 3423
Fragments arranged by size:
3423 1010 977 863 599 526 483 415
69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3827 6952 7326 7472 387
Size: 1064 138 2238 3125 374 146 1280
Fragments arranged by size:
3125 2238 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2902 2902
Size: 8365
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2271 2369 2763 3051 3162 3734 4979
Size: 393 639 98 394 288 111 572 1245
Cuts at: 4979 5139 5656 6428 7025 7812 8080 1239
Size: 160 517 772 597 787 268 1524
Fragments arranged by size:
1524 1245 787 772 639 597 572 517
394 393 288 268 160 111 98
EcoNI CCTnn'n_nnAGG
Cuts at: 3812 3812
Size: 8365
EcoO109I rG'GnC_Cy
Cuts at: 2054 2146 2637 3310 4406 4899 4900 4915 2054
Size: 92 491 673 1096 493 1 15 5504
Fragments arranged by size:
5504 1096 673 493 491 92 15 1
EcoRI G'AATT_C
Cuts at: 5674 6052 5674
Size: 378 7987
EcoRV GAT'ATC
Cuts at: 2669 4330 2669
Size: 1661 6704
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 3646 2374
Size: 1272 7093
FspI TGC'GCA
Cuts at: 6871 6871
Size: 8365
HaeII r_GCGC'y
Cuts at: 1678 3175 7512 1678
Size: 1497 4337 2531
Fragments arranged by size:
4337 2531 1497
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2072 2104 2671
Size: 32 135 32 450 32 18 32 567
Cuts at: 2671 2677 2703 2709 3761 3793 4255 4287 4811
Size: 6 26 6 1052 32 462 32 524
Cuts at: 4811 4843 7006 7038 7080 7112 1373
Size: 32 2163 32 42 32 2626
Fragments arranged by size:
2626 2163 1052 567 524 462 450 135
42 32 32 32 32 32 32 32
32 32 26 18 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3535 5709 5955 669
Size: 988 281 1597 2174 246 3079
Fragments arranged by size:
3079 2174 1597 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2454 748
Size: 1706 6659
HpaI GTT'AAC
Cuts at: 5955 5955
Size: 8365
KpnI G_GTAC'C
Cuts at: 5690 5690
Size: 8365
MfeI C'AATT_G
Cuts at: 3722 5964 3722
Size: 2242 6123
MluI A'CGCG_T
Cuts at: 8241 8241
Size: 8365
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2025 2385 4035 5202 5680 7479 7663 1305
Size: 720 360 1650 1167 478 1799 184 2007
Fragments arranged by size:
2007 1799 1650 1167 720 478 360 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2924 3452 3884 10
Size: 54 1425 343 1092 528 432 4491
Fragments arranged by size:
4491 1425 1092 528 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3377 4086 4088 4751 4874 4991
Size: 648 843 1368 709 2 663 123 117
Cuts at: 4991 6323 6682 6841 518
Size: 1332 359 159 2042
Fragments arranged by size:
2042 1368 1332 843 709 663 648 359
159 123 117 2
MspA1I CmG'CkG
Cuts at: 1606 2060 2854 3484 4885 5781 6458 7606 7851
Size: 454 794 630 1401 896 677 1148 245
Cuts at: 7851 1606
Size: 2120
Fragments arranged by size:
2120 1401 1148 896 794 677 630 454
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2022 3510 4806 4875 5183 513
Size: 552 939 18 1488 1296 69 308 3695
Fragments arranged by size:
3695 1488 1296 939 552 308 69 18
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8365
NheI G'CTAG_C
Cuts at: 1052 5140 1052
Size: 4088 4277
NotI GC'GGCC_GC
Cuts at: 5812 5812
Size: 8365
NspI r_CATG'y
Cuts at: 1132 1229 2765 3629 5491 5723 7268 1132
Size: 97 1536 864 1862 232 1545 2229
Fragments arranged by size:
2229 1862 1545 1536 864 232 97
PasI CC'CwG_GG
Cuts at: 1348 4402 4691 1348
Size: 3054 289 5022
Fragments arranged by size:
5022 3054 289
PciI A'CATG_T
Cuts at: 7264 7264
Size: 8365
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3808 3928 4627 5184 5398 5464 5634 1108
Size: 2700 120 699 557 214 66 170 3839
Fragments arranged by size:
3839 2700 699 557 214 170 120 66
PfoI T'CCnGG_A
Cuts at: 2633 2675 5156 2633
Size: 42 2481 5842
Fragments arranged by size:
5842 2481 42
PmeI GTTT'AAAC
Cuts at: 5669 5669
Size: 8365
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6434 6466 7947 7979 6434
Size: 32 1481 32 6820
Fragments arranged by size:
6820 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2146 2637 3310 4406 2146
Size: 491 673 1096 6105
Fragments arranged by size:
6105 1096 673 491
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8365
PsiI TTA'TAA
Cuts at: 2808 5935 2808
Size: 3127 5238
PspOMI G'GGCC_C
Cuts at: 4899 5226 4899
Size: 327 8038
PspXI vC'TCGA_Gb
Cuts at: 2078 2078
Size: 8365
PstI C_TGCA'G
Cuts at: 830 5009 5330 5717 830
Size: 4179 321 387 3478
Fragments arranged by size:
4179 3478 387 321
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8365
PvuII CAG'CTG
Cuts at: 2854 4885 2854
Size: 2031 6334
SacI G_AGCT'C
Cuts at: 2904 2904
Size: 8365
SacII CC_GC'GG
Cuts at: 2061 2061
Size: 8365
SalI G'TCGA_C
Cuts at: 1936 5707 1936
Size: 3771 4594
SapI GCTCTTCn'nnn_
Cuts at: 2778 3330 4455 2778
Size: 552 1125 6688
Fragments arranged by size:
6688 1125 552
SbfI CC_TGCA'GG
Cuts at: 5717 5717
Size: 8365
ScaI AGT'ACT
Cuts at: 6613 8325 6613
Size: 1712 6653
SexAI A'CCwGG_T
Cuts at: 4321 4321
Size: 8365
SfcI C'TryA_G
Cuts at: 826 1045 2016 5005 5059 5326 5713 6848 7529
Size: 219 971 2989 54 267 387 1135 681
Cuts at: 7529 7720 826
Size: 191 1471
Fragments arranged by size:
2989 1471 1135 971 681 387 267 219
191 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8365
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8365
SmaI CCC'GGG
Cuts at: 1872 2642 5692 1872
Size: 770 3050 4545
Fragments arranged by size:
4545 3050 770
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 3314 3680 4799 5646 6469
Size: 197 926 135 1236 366 1119 847 823
Cuts at: 6469 7370 7632 820
Size: 901 262 1553
Fragments arranged by size:
1553 1236 1119 926 901 847 823 366
262 197 135
SnaBI TAC'GTA
Cuts at: 493 6156 493
Size: 5663 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8365
SphI G_CATG'C
Cuts at: 1132 3629 5723 1132
Size: 2497 2094 3774
Fragments arranged by size:
3774 2497 2094
SspI AAT'ATT
Cuts at: 5 52 6289 5
Size: 47 6237 2081
Fragments arranged by size:
6237 2081 47
StuI AGG'CCT
Cuts at: 1517 2883 1517
Size: 1366 6999
StyI C'CwwG_G
Cuts at: 513 1065 2004 2022 3447 3510 4161 4806 4875
Size: 552 939 18 1425 63 651 645 69
Cuts at: 4875 5067 5183 5478 5804 513
Size: 192 116 295 326 3074
Fragments arranged by size:
3074 1425 939 651 645 552 326 295
192 116 69 63 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2661 3815 3834 5437 6392 6409 6562
Size: 1255 1081 1154 19 1603 955 17 153
Cuts at: 6562 6747 7192 325
Size: 185 445 1498
Fragments arranged by size:
1603 1498 1255 1154 1081 955 445 185
153 19 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3981 4245 4440
Size: 274 80 33 51 1443 2004 264 195
Cuts at: 4440 4710 4887 5148 5607 6611 8323 96
Size: 270 177 261 459 1004 1712 138
Fragments arranged by size:
2004 1712 1443 1004 459 274 270 264
261 195 177 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8325 8357 8325
Size: 32 8333
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8365
XbaI T'CTAG_A
Cuts at: 2663 5701 2663
Size: 3038 5327
XhoI C'TCGA_G
Cuts at: 1943 2078 5646 1943
Size: 135 3568 4662
Fragments arranged by size:
4662 3568 135
XmaI C'CCGG_G
Cuts at: 1870 2640 5690 1870
Size: 770 3050 4545
Fragments arranged by size:
4545 3050 770
XmnI GAAnn'nnTTC
Cuts at: 3249 5054 6494 3249
Size: 1805 1440 5120
Fragments arranged by size:
5120 1805 1440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6173 276
Size: 53 83 186 936 4639 2468
Fragments arranged by size:
4639 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PsrI RsrII SanDI SfiI SfoI SrfI SwaI
XcmI