Restriction map for
pFN21AB0174
(Circular) (Six-base) MAPSORT of: pFN21AB0174.seq Check: 5516 from: 1 to: 8779
pFN21AB0174 8779 bp
With 182 enzymes: SgfI *
March 28, 2012 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3715 6589 278
Size: 53 83 186 936 2179 2874 2468
Fragments arranged by size:
2874 2468 2179 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2257 2673 4077 6122 1937
Size: 320 416 1404 2045 4594
Fragments arranged by size:
4594 2045 1404 416 320
Acc65I G'GTAC_C
Cuts at: 5492 6100 5492
Size: 608 8171
AclI AA'CG_TT
Cuts at: 1563 2605 2905 3008 6906 7279 1563
Size: 1042 300 103 3898 373 3063
Fragments arranged by size:
3898 3063 1042 373 300 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2960 3330 4337 4820 5919 6842 8226 8494
Size: 1328 370 1007 483 1099 923 1384 268
Cuts at: 8494 1632
Size: 1917
Fragments arranged by size:
1917 1384 1328 1099 1007 923 483 370
268
AfeI AGC'GCT
Cuts at: 2381 2381
Size: 8779
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8582
AflIII A'CryG_T
Cuts at: 1203 4546 5033 7678 8655 1203
Size: 3343 487 2645 977 1327
Fragments arranged by size:
3343 2645 1327 977 487
AgeI A'CCGG_T
Cuts at: 4768 7610 4768
Size: 2842 5937
AhdI GACnn_n'nnGTC
Cuts at: 5383 7508 5383
Size: 2125 6654
AleI CACnn'nnGTG
Cuts at: 1166 3694 1166
Size: 2528 6251
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4887 5001 1184
Size: 3703 114 4962
Fragments arranged by size:
4962 3703 114
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5501 5533 5501
Size: 32 8747
AlwNI CAG_nnn'CTG
Cuts at: 2401 4315 6522 8094 2401
Size: 1914 2207 1572 3086
Fragments arranged by size:
3086 2207 1914 1572
ApaI G_GGCC'C
Cuts at: 5966 5966
Size: 8779
ApaLI G'TGCA_C
Cuts at: 5688 6836 7992 5688
Size: 1148 1156 6475
Fragments arranged by size:
6475 1156 1148
ApoI r'AATT_y
Cuts at: 1493 3890 3968 5268 5913 6088 6314 6466 8575
Size: 2397 78 1300 645 175 226 152 2109
Cuts at: 8575 1493
Size: 1697
Fragments arranged by size:
2397 2109 1697 1300 645 226 175 152
78
AseI AT'TA_AT
Cuts at: 160 1032 7333 160
Size: 872 6301 1606
Fragments arranged by size:
6301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8779
AvaI C'yCGr_G
Cuts at: 1870 1943 2127 6104 1870
Size: 73 184 3977 4545
Fragments arranged by size:
4545 3977 184 73
AvrII C'CTAG_G
Cuts at: 3296 3296
Size: 8779
BamHI G'GATC_C
Cuts at: 2179 4294 6109 6476 2179
Size: 2115 1815 367 4482
Fragments arranged by size:
4482 2115 1815 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4025 5492 6100 7555 618
Size: 325 217 627 2238 1467 608 1455 1842
Fragments arranged by size:
2238 1842 1467 1455 627 608 325 217
BanII G_rGCy'C
Cuts at: 1391 3594 5560 5966 6098 1391
Size: 2203 1966 406 132 4072
Fragments arranged by size:
4072 2203 1966 406 132
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2541 4362 5093 5379 928
Size: 971 642 1821 731 286 4328
Fragments arranged by size:
4328 1821 971 731 642 286
BbvCI CC'TCA_GC
Cuts at: 3885 3885
Size: 8779
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4670 4704 6970 7004 1903
Size: 34 2733 34 2266 34 3678
Fragments arranged by size:
3678 2733 2266 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4008 6671 7881 4008
Size: 2663 1210 4906
Fragments arranged by size:
4906 2663 1210
BclI T'GATC_A
Cuts at: 1548 4642 1548
Size: 3094 5685
BfrBI ATG'CAT
Cuts at: 5308 5308
Size: 8779
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7390 136
Size: 107 122 71 631 605 5718 1525
Fragments arranged by size:
5718 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5102 8774 5102
Size: 3672 5107
BlpI GC'TnA_GC
Cuts at: 2751 4887 6196 2751
Size: 2136 1309 5334
Fragments arranged by size:
5334 2136 1309
Bme1580I G_kGCm'C
Cuts at: 1790 5692 5966 6840 7996 1790
Size: 3902 274 874 1156 2573
Fragments arranged by size:
3902 2573 1156 874 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2986 4494 4671
Size: 416 53 83 399 24 1569 1508 177
Cuts at: 4671 6510 7468 442
Size: 1839 958 1753
Fragments arranged by size:
1839 1753 1569 1508 958 416 399 177
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8779
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2348 2348
Size: 8779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2237 2451 5343 7439 1239
Size: 998 214 2892 2096 2579
Fragments arranged by size:
2892 2579 2096 998 214
Bpu10I CC'TnA_GC
Cuts at: 3885 3885
Size: 8779
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3337 3836 6904 7769 8067 3337
Size: 499 3068 865 298 4049
Fragments arranged by size:
4049 3068 865 499 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7442 882
Size: 617 5943 2219
Fragments arranged by size:
5943 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3785 6570 493
Size: 711 2581 2785 2702
Fragments arranged by size:
2785 2702 2581 711
BsaBI GATnn'nnATC
Cuts at: 3777 5307 5760 8773 3777
Size: 1530 453 3013 3783
Fragments arranged by size:
3783 3013 1530 453
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3712 4942 5569 6586
Size: 53 83 186 936 2179 1230 627 1017
Cuts at: 6586 6968 7603 275
Size: 382 635 1451
Fragments arranged by size:
2179 1451 1230 1017 936 635 627 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3382 4768 7212 7610 7884 8031 8442 3382
Size: 1386 2444 398 274 147 411 3719
Fragments arranged by size:
3719 2444 1386 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5121 5151 1178
Size: 30 165 30 3718 30 4806
Fragments arranged by size:
4806 3718 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2593 2715 3832 4662 5709 1186
Size: 1407 122 1117 830 1047 4256
Fragments arranged by size:
4256 1407 1117 1047 830 122
BseYI C'CCAG_C
Cuts at: 5373 7982 8478 5373
Size: 2609 496 5674
Fragments arranged by size:
5674 2609 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2229 1163
Size: 573 493 7713
Fragments arranged by size:
7713 573 493
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6229 6990 8018 1540
Size: 275 186 4228 761 1028 2301
Fragments arranged by size:
4228 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3594 5329 5692 6840 6925 7996 3594
Size: 1735 363 1148 85 1071 4377
Fragments arranged by size:
4377 1735 1148 1071 363 85
BsmI GAATG_Cn'
Cuts at: 6290 6383 6290
Size: 93 8686
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3594 5329 5560 5692 5966 6098 6840
Size: 399 1804 1735 231 132 274 132 742
Cuts at: 6840 6925 7996 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1804 1735 1071 742 399 274 231
132 132 85
BspEI T'CCGG_A
Cuts at: 3382 8442 3382
Size: 5060 3719
BspHI T'CATG_A
Cuts at: 2074 4383 4435 4972 5989 6668 8398 2074
Size: 2309 52 537 1017 679 1730 2455
Fragments arranged by size:
2455 2309 1730 1017 679 537 52
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2726 6134 844
Size: 1882 3408 3489
Fragments arranged by size:
3489 3408 1882
BsrBI CCG'CTC
Cuts at: 1394 1823 6666 1394
Size: 429 4843 3507
Fragments arranged by size:
4843 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2025 2186 2724 2950 3289 4876 5401
Size: 1183 777 161 538 226 339 1587 525
Cuts at: 5401 7274 7448 65
Size: 1873 174 1396
Fragments arranged by size:
1873 1587 1396 1183 777 538 525 339
226 174 161
BsrFI r'CCGG_y
Cuts at: 1700 1810 4768 7423 7610 1700
Size: 110 2958 2655 187 2869
Fragments arranged by size:
2958 2869 2655 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8779
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8779
BssSI C'ACGA_G
Cuts at: 6839 7851 6839
Size: 1012 7767
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2320 794
Size: 1526 7253
BstBI TT'CG_AA
Cuts at: 4810 7583 4810
Size: 2773 6006
BstEII G'GTnAC_C
Cuts at: 1184 2470 1184
Size: 1286 7493
BstXI CCAn_nnnn'nTGG
Cuts at: 4417 4938 5210 4417
Size: 521 272 7986
Fragments arranged by size:
7986 521 272
BstYI r'GATC_y
Cuts at: 1972 2040 2179 2397 2653 4294 5102 6109 6476
Size: 68 139 218 256 1641 808 1007 367
Cuts at: 6476 6861 6878 8330 8416 8428 8774 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1641 1452 1007 808 385 367 346
256 218 139 86 68 17 12
Bsu36I CC'TnA_GG
Cuts at: 3114 5381 3114
Size: 2267 6512
BtgI C'CryG_G
Cuts at: 513 1065 2004 4029 5496 5731 5979 513
Size: 552 939 2025 1467 235 248 3313
Fragments arranged by size:
3313 2025 1467 939 552 248 235
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8040
Fragments arranged by size:
8040 683 56
BtsI GCAGTG_nn'
Cuts at: 4871 5494 5692 6295 7089 7109 4871
Size: 623 198 603 794 20 6541
Fragments arranged by size:
6541 794 623 603 198 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8779
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3058 3093 529
Size: 35 2494 35 6215
Fragments arranged by size:
6215 2494 35 35
DraI TTT'AAA
Cuts at: 2119 4164 5409 5421 6083 2119
Size: 2045 1245 12 662 4815
Fragments arranged by size:
4815 2045 1245 662 12
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7786 809
Size: 507 6470 1802
Fragments arranged by size:
6470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6226 7115 8744
Size: 54 1425 50 275 18 4396 889 1629
Cuts at: 8744 8
Size: 43
Fragments arranged by size:
4396 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6226 1537
Size: 275 4414 4090
Fragments arranged by size:
4414 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2705 4275 4344 6709 1353
Size: 1352 1570 69 2365 3423
Fragments arranged by size:
3423 2365 1570 1352 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4275 7366 7740 7886 387
Size: 1064 138 2686 3091 374 146 1280
Fragments arranged by size:
3091 2686 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3592 3592
Size: 8779
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2237 2451 2960 3330 4337 4820 5343
Size: 393 605 214 509 370 1007 483 523
Cuts at: 5343 5919 6842 7439 8226 8494 1239
Size: 576 923 597 787 268 1524
Fragments arranged by size:
1524 1007 923 787 605 597 576 523
509 483 393 370 268 214
EcoNI CCTnn'n_nnAGG
Cuts at: 5875 5875
Size: 8779
EcoO109I rG'GnC_Cy
Cuts at: 4624 5377 5829 5962 4624
Size: 753 452 133 7441
Fragments arranged by size:
7441 753 452 133
EcoRI G'AATT_C
Cuts at: 5268 6088 6466 5268
Size: 820 378 7581
Fragments arranged by size:
7581 820 378
EcoRV GAT'ATC
Cuts at: 3370 3370
Size: 8779
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4379 4517 4379
Size: 138 8641
FspI TGC'GCA
Cuts at: 7285 7285
Size: 8779
HaeII r_GCGC'y
Cuts at: 1678 2383 7926 1678
Size: 705 5543 2531
Fragments arranged by size:
5543 2531 705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4081 4113 4967 4999 5003
Size: 32 135 32 2509 32 854 32 4
Cuts at: 5003 5035 5121 5153 5369 5401 5408 5440 5658
Size: 32 86 32 216 32 7 32 218
Cuts at: 5658 5690 7420 7452 7494 7526 1373
Size: 32 1730 32 42 32 2626
Fragments arranged by size:
2626 2509 1730 854 218 216 135 86
42 32 32 32 32 32 32 32
32 32 32 32 7 4
HincII GTy'rAC
Cuts at: 669 1657 1938 3327 4713 6123 6369 669
Size: 988 281 1389 1386 1410 246 3079
Fragments arranged by size:
3079 1410 1389 1386 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8779
HpaI GTT'AAC
Cuts at: 6369 6369
Size: 8779
KpnI G_GTAC'C
Cuts at: 5496 6104 5496
Size: 608 8171
MfeI C'AATT_G
Cuts at: 6378 6378
Size: 8779
MluI A'CGCG_T
Cuts at: 8655 8655
Size: 8779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2459 7893 8077 1305
Size: 1154 5434 184 2007
Fragments arranged by size:
5434 2007 1154 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6957
Fragments arranged by size:
6957 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3570 3694 3783 4434 4936 4971 6737
Size: 648 2404 124 89 651 502 35 1766
Cuts at: 6737 7096 7255 518
Size: 359 159 2042
Fragments arranged by size:
2404 2042 1766 651 648 502 359 159
124 89 35
MspA1I CmG'CkG
Cuts at: 1606 2408 4070 5612 5780 6195 6872 8020 8265
Size: 802 1662 1542 168 415 677 1148 245
Cuts at: 8265 1606
Size: 2120
Fragments arranged by size:
2120 1662 1542 1148 802 677 415 245
168
NcoI C'CATG_G
Cuts at: 513 1065 2004 5496 5731 513
Size: 552 939 3492 235 3561
Fragments arranged by size:
3561 3492 939 552 235
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8779
NotI GC'GGCC_GC
Cuts at: 6226 6226
Size: 8779
NsiI A_TGCA'T
Cuts at: 5310 5310
Size: 8779
NspI r_CATG'y
Cuts at: 1132 1229 4246 4550 4591 4618 5037 6137 7682
Size: 97 3017 304 41 27 419 1100 1545
Cuts at: 7682 1132
Size: 2229
Fragments arranged by size:
3017 2229 1545 1100 419 304 97 41
27
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8779
PciI A'CATG_T
Cuts at: 4546 5033 7678 4546
Size: 487 2645 5647
Fragments arranged by size:
5647 2645 487
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4011 4934 1108
Size: 2903 923 4953
Fragments arranged by size:
4953 2903 923
PfoI T'CCnGG_A
Cuts at: 3492 5907 3492
Size: 2415 6364
PmeI GTTT'AAAC
Cuts at: 6083 6083
Size: 8779
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6848 6880 8361 8393 6848
Size: 32 1481 32 7234
Fragments arranged by size:
7234 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5377 5829 5377
Size: 452 8327
PshAI GACnn'nnGTC
Cuts at: 1613 4742 1613
Size: 3129 5650
PsiI TTA'TAA
Cuts at: 3149 6349 3149
Size: 3200 5579
PspOMI G'GGCC_C
Cuts at: 5962 5962
Size: 8779
PspXI vC'TCGA_Gb
Cuts at: 2127 2127
Size: 8779
PstI C_TGCA'G
Cuts at: 830 3022 5755 6131 830
Size: 2192 2733 376 3478
Fragments arranged by size:
3478 2733 2192 376
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8779
PvuII CAG'CTG
Cuts at: 2408 4070 5612 5780 2408
Size: 1662 1542 168 5407
Fragments arranged by size:
5407 1662 1542 168
SacI G_AGCT'C
Cuts at: 3594 3594
Size: 8779
SalI G'TCGA_C
Cuts at: 1936 6121 1936
Size: 4185 4594
SbfI CC_TGCA'GG
Cuts at: 6131 6131
Size: 8779
ScaI AGT'ACT
Cuts at: 2107 5851 7027 8739 2107
Size: 3744 1176 1712 2147
Fragments arranged by size:
3744 2147 1712 1176
SfcI C'TryA_G
Cuts at: 826 1045 2049 3018 3161 5743 5751 5875 6127
Size: 219 1004 969 143 2582 8 124 252
Cuts at: 6127 7262 7943 8134 826
Size: 1135 681 191 1471
Fragments arranged by size:
2582 1471 1135 1004 969 681 252 219
191 143 124 8
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8779
SmaI CCC'GGG
Cuts at: 1872 6106 1872
Size: 4234 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2127 3316 3851 6883 7784 8046
Size: 197 926 184 1189 535 3032 901 262
Cuts at: 8046 820
Size: 1553
Fragments arranged by size:
3032 1553 1189 926 901 535 262 197
184
SnaBI TAC'GTA
Cuts at: 493 6570 493
Size: 6077 2702
SpeI A'CTAG_T
Cuts at: 152 5852 152
Size: 5700 3079
SphI G_CATG'C
Cuts at: 1132 6137 1132
Size: 5005 3774
SspI AAT'ATT
Cuts at: 5 52 2961 3376 3655 6703 5
Size: 47 2909 415 279 3048 2081
Fragments arranged by size:
3048 2909 2081 415 279 47
StuI AGG'CCT
Cuts at: 1517 2148 1517
Size: 631 8148
StyI C'CwwG_G
Cuts at: 513 1065 2004 3000 3296 5158 5496 5731 6218
Size: 552 939 996 296 1862 338 235 487
Cuts at: 6218 513
Size: 3074
Fragments arranged by size:
3074 1862 996 939 552 487 338 296
235
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5590 6806 6823 6976 7161 7606 325
Size: 1255 4010 1216 17 153 185 445 1498
Fragments arranged by size:
4010 1498 1255 1216 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2105 3502 4064
Size: 274 80 33 51 1443 128 1397 562
Cuts at: 4064 4781 5849 7025 8737 96
Size: 717 1068 1176 1712 138
Fragments arranged by size:
1712 1443 1397 1176 1068 717 562 274
138 128 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2512 2544 8739 8771 2512
Size: 32 6195 32 2520
Fragments arranged by size:
6195 2520 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8779
XbaI T'CTAG_A
Cuts at: 5272 6050 6115 5272
Size: 778 65 7936
Fragments arranged by size:
7936 778 65
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4935 5644 4935
Size: 709 8070
XhoI C'TCGA_G
Cuts at: 1943 2127 1943
Size: 184 8595
XmaI C'CCGG_G
Cuts at: 1870 6104 1870
Size: 4234 4545
XmnI GAAnn'nnTTC
Cuts at: 3009 3207 3390 4264 4379 6908 3009
Size: 198 183 874 115 2529 4880
Fragments arranged by size:
4880 2529 874 198 183 115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3713 6587 276
Size: 53 83 186 936 2179 2874 2468
Fragments arranged by size:
2874 2468 2179 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BsiWI BsmBI BstZ17I DraIII
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PmlI PsrI RsrII SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI