Restriction map for
pFN21AB0150
(Circular) (Six-base) MAPSORT of: pFN21AB0150.seq Check: 6804 from: 1 to: 9097
pFN21AB0150 9097 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6907 278
Size: 53 83 186 936 5371 2468
Fragments arranged by size:
5371 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4034 6440 1937
Size: 2097 2406 4594
Fragments arranged by size:
4594 2406 2097
Acc65I G'GTAC_C
Cuts at: 6418 6418
Size: 9097
AclI AA'CG_TT
Cuts at: 1563 2409 7224 7597 1563
Size: 846 4815 373 3063
Fragments arranged by size:
4815 3063 846 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3599 3804 4994 5567 7160 8544 8812 1632
Size: 1967 205 1190 573 1593 1384 268 1917
Fragments arranged by size:
1967 1917 1593 1384 1190 573 268 205
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8900
AflIII A'CryG_T
Cuts at: 1203 7996 8973 1203
Size: 6793 977 1327
Fragments arranged by size:
6793 1327 977
AgeI A'CCGG_T
Cuts at: 7928 7928
Size: 9097
AhdI GACnn_n'nnGTC
Cuts at: 5140 7826 5140
Size: 2686 6411
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4664 1184
Size: 3480 5617
AlwNI CAG_nnn'CTG
Cuts at: 2276 3226 3937 4175 4328 5707 6840 8412 2276
Size: 950 711 238 153 1379 1133 1572 2961
Fragments arranged by size:
2961 1572 1379 1133 950 711 238 153
ApaLI G'TGCA_C
Cuts at: 7154 8310 7154
Size: 1156 7941
ApoI r'AATT_y
Cuts at: 1493 2170 2241 2513 3274 4068 4596 4660 4795
Size: 677 71 272 761 794 528 64 135
Cuts at: 4795 5269 6406 6632 6784 8893 1493
Size: 474 1137 226 152 2109 1697
Fragments arranged by size:
2109 1697 1137 794 761 677 528 474
272 226 152 135 71 64
AseI AT'TA_AT
Cuts at: 160 1032 4346 5694 7651 160
Size: 872 3314 1348 1957 1606
Fragments arranged by size:
3314 1957 1606 1348 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9097
AvaI C'yCGr_G
Cuts at: 1870 1943 6290 6422 1870
Size: 73 4347 132 4545
Fragments arranged by size:
4545 4347 132 73
BamHI G'GATC_C
Cuts at: 6427 6794 6427
Size: 367 8730
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4487 4867 5392 5686 6418
Size: 325 217 627 2700 380 525 294 732
Cuts at: 6418 7873 618
Size: 1455 1842
Fragments arranged by size:
2700 1842 1455 732 627 525 380 325
294 217
BanII G_rGCy'C
Cuts at: 1391 3485 3780 3966 4214 4579 5239 5975 6416
Size: 2094 295 186 248 365 660 736 441
Cuts at: 6416 1391
Size: 4072
Fragments arranged by size:
4072 2094 736 660 441 365 295 248
186
BbeI G_GCGC'C
Cuts at: 4491 5396 4491
Size: 905 8192
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 8126
BbvCI CC'TCA_GC
Cuts at: 3623 4749 3623
Size: 1126 7971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2324 2358 7288 7322 1903
Size: 34 387 34 4930 34 3678
Fragments arranged by size:
4930 3678 387 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2320 2848 3985 6989 8199 2320
Size: 528 1137 3004 1210 3218
Fragments arranged by size:
3218 3004 1210 1137 528
BclI T'GATC_A
Cuts at: 1548 3952 1548
Size: 2404 6693
BfrBI ATG'CAT
Cuts at: 5417 5761 5417
Size: 344 8753
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3221 4992 6047
Size: 107 122 71 631 605 1549 1771 1055
Cuts at: 6047 7708 136
Size: 1661 1525
Fragments arranged by size:
1771 1661 1549 1525 1055 631 605 122
107 71
BglII A'GATC_T
Cuts at: 9092 9092
Size: 9097
BlpI GC'TnA_GC
Cuts at: 6514 6514
Size: 9097
Bme1580I G_kGCm'C
Cuts at: 1790 3636 4870 7158 8314 1790
Size: 1846 1234 2288 1156 2573
Fragments arranged by size:
2573 2288 1846 1234 1156
BmgBI CAC'GTC
Cuts at: 1318 2847 1318
Size: 1529 7568
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3510 3695 4646
Size: 416 53 83 399 24 2093 185 951
Cuts at: 4646 5961 6828 7786 442
Size: 1315 867 958 1753
Fragments arranged by size:
2093 1753 1315 958 951 867 416 399
185 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9097
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5886 5886
Size: 9097
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2023 3507 3843 4878 5166 5303 5402 5691
Size: 784 1484 336 1035 288 137 99 289
Cuts at: 5691 7757 1239
Size: 2066 2579
Fragments arranged by size:
2579 2066 1484 1035 784 336 289 288
137 99
Bpu10I CC'TnA_GC
Cuts at: 3623 3973 4148 4749 3623
Size: 350 175 601 7971
Fragments arranged by size:
7971 601 350 175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2384 4037 4322 5341 7222 8087 8385 2384
Size: 1653 285 1019 1881 865 298 3096
Fragments arranged by size:
3096 1881 1653 1019 865 298 285
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3646 7760 882
Size: 617 2147 4114 2219
Fragments arranged by size:
4114 2219 2147 617
BsaAI yAC'GTr
Cuts at: 493 1204 6888 493
Size: 711 5684 2702
Fragments arranged by size:
5684 2702 711
BsaBI GATnn'nnATC
Cuts at: 9091 9091
Size: 9097
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4488 5141 5393 6904
Size: 53 83 186 936 2955 653 252 1511
Cuts at: 6904 7286 7921 275
Size: 382 635 1451
Fragments arranged by size:
2955 1511 1451 936 653 635 382 252
186 83 53
BsaWI w'CCGG_w
Cuts at: 3154 7530 7928 8202 8349 8760 3154
Size: 4376 398 274 147 411 3491
Fragments arranged by size:
4376 3491 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2934 2964 3989 4019 1178
Size: 30 165 30 1531 30 1025 30 6256
Fragments arranged by size:
6256 1531 1025 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 3366 4385 4439 5079 5300 1186
Size: 1468 712 1019 54 640 221 4983
Fragments arranged by size:
4983 1468 1019 712 640 221 54
BseYI C'CCAG_C
Cuts at: 2087 2347 2889 4954 4979 6037 6108 8300 8796
Size: 260 542 2065 25 1058 71 2192 496
Cuts at: 8796 2087
Size: 2388
Fragments arranged by size:
2388 2192 2065 1058 542 496 260 71
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2315 3033 3250 5570 1163
Size: 573 579 718 217 2320 4690
Fragments arranged by size:
4690 2320 718 579 573 217
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3864 6547 7308 8336 1540
Size: 275 186 1863 2683 761 1028 2301
Fragments arranged by size:
2683 2301 1863 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4610 5239 7158 7243 8314 4610
Size: 629 1919 85 1071 5393
Fragments arranged by size:
5393 1919 1071 629 85
BsmI GAATG_Cn'
Cuts at: 2999 3544 4234 6608 6701 2999
Size: 545 690 2374 93 5395
Fragments arranged by size:
5395 2374 690 545 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2853 2853
Size: 9097
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3485 3636 3780 3966 4214 4579 4610
Size: 399 1695 151 144 186 248 365 31
Cuts at: 4610 4870 5239 5975 6416 7158 7243 8314 1391
Size: 260 369 736 441 742 85 1071 2174
Fragments arranged by size:
2174 1695 1071 742 736 441 399 369
365 260 248 186 151 144 85 31
BspEI T'CCGG_A
Cuts at: 3154 8760 3154
Size: 5606 3491
BspHI T'CATG_A
Cuts at: 2647 2974 5022 6986 8716 2647
Size: 327 2048 1964 1730 3028
Fragments arranged by size:
3028 2048 1964 1730 327
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2535 3312 3861 4632 6286 6452 844
Size: 1691 777 549 771 1654 166 3489
Fragments arranged by size:
3489 1691 1654 777 771 549 166
BsrBI CCG'CTC
Cuts at: 1394 1823 6984 1394
Size: 429 5161 3507
Fragments arranged by size:
5161 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4359 4679 5644 7592 7766 65
Size: 1183 3111 320 965 1948 174 1396
Fragments arranged by size:
3111 1948 1396 1183 965 320 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4204 4923 7741 7928 1700
Size: 110 2394 719 2818 187 2869
Fragments arranged by size:
2869 2818 2394 719 187 110
BsrGI T'GTAC_A
Cuts at: 96 6153 96
Size: 6057 3040
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9097
BssSI C'ACGA_G
Cuts at: 4477 7157 8169 4477
Size: 2680 1012 5405
Fragments arranged by size:
5405 2680 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2276 794
Size: 1482 7615
BstBI TT'CG_AA
Cuts at: 7901 7901
Size: 9097
BstEII G'GTnAC_C
Cuts at: 1184 4391 1184
Size: 3207 5890
BstXI CCAn_nnnn'nTGG
Cuts at: 6347 6347
Size: 9097
BstYI r'GATC_y
Cuts at: 1972 2555 2827 3157 4324 5341 5461 6427 6794
Size: 583 272 330 1167 1017 120 966 367
Cuts at: 6794 7179 7196 8648 8734 8746 9092 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1167 1017 966 583 385 367
346 330 272 120 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3378 5302 5531 3378
Size: 1924 229 6944
Fragments arranged by size:
6944 1924 229
BtgI C'CryG_G
Cuts at: 513 1065 5108 5478 5538 6183 6282 513
Size: 552 4043 370 60 645 99 3328
Fragments arranged by size:
4043 3328 645 552 370 99 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3458 5573 487
Size: 683 56 2232 2115 4011
Fragments arranged by size:
4011 2232 2115 683 56
BtsI GCAGTG_nn'
Cuts at: 2693 3238 3877 4411 4589 6613 7407 7427 2693
Size: 545 639 534 178 2024 794 20 4363
Fragments arranged by size:
4363 2024 794 639 545 534 178 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9097
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5814 5849 6181 6216 529
Size: 35 5250 35 332 35 3410
Fragments arranged by size:
5250 3410 332 35 35 35
DraI TTT'AAA
Cuts at: 6401 6401
Size: 9097
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5011 8104 809
Size: 507 3695 3093 1802
Fragments arranged by size:
3695 3093 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3861 4113 4925
Size: 54 1425 50 275 18 2031 252 812
Cuts at: 4925 4993 6544 7433 9062 8
Size: 68 1551 889 1629 43
Fragments arranged by size:
2031 1629 1551 1425 889 812 275 252
68 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3861 6544 1537
Size: 275 2049 2683 4090
Fragments arranged by size:
4090 2683 2049 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 2631 2761 3053 7027 1353
Size: 1101 177 130 292 3974 3423
Fragments arranged by size:
3974 3423 1101 292 177 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2596 6105 7684 8058 8204 387
Size: 1064 138 1007 3509 1579 374 146 1280
Fragments arranged by size:
3509 1579 1280 1064 1007 374 146 138
EcoICRI GAG'CTC
Cuts at: 5237 5237
Size: 9097
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2023 3507 3599 3804 3843 4878 4994
Size: 393 391 1484 92 205 39 1035 116
Cuts at: 4994 5166 5303 5402 5567 5691 7160 7757 8544
Size: 172 137 99 165 124 1469 597 787
Cuts at: 8544 8812 1239
Size: 268 1524
Fragments arranged by size:
1524 1484 1469 1035 787 597 393 391
268 205 172 165 137 124 116 99
92 39
EcoNI CCTnn'n_nnAGG
Cuts at: 3626 4151 4752 5940 6267 3626
Size: 525 601 1188 327 6456
Fragments arranged by size:
6456 1188 601 525 327
EcoO109I rG'GnC_Cy
Cuts at: 2351 2368 2896 3223 3619 5063 6049 6287 2351
Size: 17 528 327 396 1444 986 238 5161
Fragments arranged by size:
5161 1444 986 528 396 327 238 17
EcoRI G'AATT_C
Cuts at: 6406 6784 6406
Size: 378 8719
FspI TGC'GCA
Cuts at: 2931 7603 2931
Size: 4672 4425
HaeII r_GCGC'y
Cuts at: 1678 4491 5396 8244 1678
Size: 2813 905 2848 2531
Fragments arranged by size:
2848 2813 2531 905
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2297 2329 2425 2457 2726
Size: 32 135 32 725 32 96 32 269
Cuts at: 2726 2758 2833 2865 2956 2988 4753 4785 5126
Size: 32 75 32 91 32 1765 32 341
Cuts at: 5126 5158 5514 5546 7738 7770 7812 7844 1373
Size: 32 356 32 2192 32 42 32 2626
Fragments arranged by size:
2626 2192 1765 725 356 341 269 135
96 91 75 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4122 4507 6441 6687 669
Size: 988 281 2184 385 1934 246 3079
Fragments arranged by size:
3079 2184 1934 988 385 281 246
HindIII A'AGCT_T
Cuts at: 748 3538 3739 748
Size: 2790 201 6106
Fragments arranged by size:
6106 2790 201
HpaI GTT'AAC
Cuts at: 4122 6687 4122
Size: 2565 6532
KasI G'GCGC_C
Cuts at: 4487 5392 4487
Size: 905 8192
KpnI G_GTAC'C
Cuts at: 6422 6422
Size: 9097
MfeI C'AATT_G
Cuts at: 2599 6696 2599
Size: 4097 5000
MluI A'CGCG_T
Cuts at: 8973 8973
Size: 9097
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2585 3969 4552 5394 6081 8211 8395 1305
Size: 1280 1384 583 842 687 2130 184 2007
Fragments arranged by size:
2130 2007 1384 1280 842 687 583 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4115 4995 10
Size: 54 1425 343 2283 880 4112
Fragments arranged by size:
4112 2283 1425 880 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2973 3455 4817 4970 6149 6176 7055
Size: 648 1807 482 1362 153 1179 27 879
Cuts at: 7055 7414 7573 518
Size: 359 159 2042
Fragments arranged by size:
2042 1807 1362 1179 879 648 482 359
159 153 27
MspA1I CmG'CkG
Cuts at: 1606 2276 3813 3934 4547 5213 5251 5488 6343
Size: 670 1537 121 613 666 38 237 855
Cuts at: 6343 6513 7190 8338 8583 1606
Size: 170 677 1148 245 2120
Fragments arranged by size:
2120 1537 1148 855 677 670 666 613
245 237 170 121 38
NarI GG'CG_CC
Cuts at: 4488 5393 4488
Size: 905 8192
NcoI C'CATG_G
Cuts at: 513 1065 5108 5478 6183 6282 513
Size: 552 4043 370 705 99 3328
Fragments arranged by size:
4043 3328 705 552 370 99
NdeI CA'TA_TG
Cuts at: 387 3117 387
Size: 2730 6367
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9097
NotI GC'GGCC_GC
Cuts at: 6544 6544
Size: 9097
NsiI A_TGCA'T
Cuts at: 5419 5763 5419
Size: 344 8753
NspI r_CATG'y
Cuts at: 1132 1229 2498 3313 3364 6103 6154 6455 8000
Size: 97 1269 815 51 2739 51 301 1545
Cuts at: 8000 1132
Size: 2229
Fragments arranged by size:
2739 2229 1545 1269 815 301 97 51
51
PasI CC'CwG_GG
Cuts at: 1348 5437 1348
Size: 4089 5008
PciI A'CATG_T
Cuts at: 7996 7996
Size: 9097
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6128 1108
Size: 5020 4077
PfoI T'CCnGG_A
Cuts at: 3439 3820 4174 5241 3439
Size: 381 354 1067 7295
Fragments arranged by size:
7295 1067 381 354
PmeI GTTT'AAAC
Cuts at: 6401 6401
Size: 9097
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3398 3430 7166 7198 8679 8711 3398
Size: 32 3736 32 1481 32 3784
Fragments arranged by size:
3784 3736 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2896 5063 6287 2896
Size: 2167 1224 5706
Fragments arranged by size:
5706 2167 1224
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9097
PsiI TTA'TAA
Cuts at: 6667 6667
Size: 9097
PstI C_TGCA'G
Cuts at: 830 3535 3933 5212 6449 830
Size: 2705 398 1279 1237 3478
Fragments arranged by size:
3478 2705 1279 1237 398
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9097
PvuII CAG'CTG
Cuts at: 2276 3813 3934 4547 5213 5251 6343 2276
Size: 1537 121 613 666 38 1092 5030
Fragments arranged by size:
5030 1537 1092 666 613 121 38
SacI G_AGCT'C
Cuts at: 5239 5239
Size: 9097
SalI G'TCGA_C
Cuts at: 1936 6439 1936
Size: 4503 4594
SanDI GG'GwC_CC
Cuts at: 6287 6287
Size: 9097
SapI GCTCTTCn'nnn_
Cuts at: 2454 3053 2454
Size: 599 8498
SbfI CC_TGCA'GG
Cuts at: 3535 6449 3535
Size: 2914 6183
ScaI AGT'ACT
Cuts at: 2982 7345 9057 2982
Size: 4363 1712 3022
Fragments arranged by size:
4363 3022 1712
SexAI A'CCwGG_T
Cuts at: 2898 2898
Size: 9097
SfcI C'TryA_G
Cuts at: 826 1045 2316 2541 3531 3929 5130 5208 6445
Size: 219 1271 225 990 398 1201 78 1237
Cuts at: 6445 7580 8261 8452 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1271 1237 1201 1135 990 681 398
225 219 191 78
SfoI GGC'GCC
Cuts at: 4489 5394 4489
Size: 905 8192
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9097
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9097
SmaI CCC'GGG
Cuts at: 1872 6424 1872
Size: 4552 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2363 4052 4301 5356 7201 8102
Size: 197 926 420 1689 249 1055 1845 901
Cuts at: 8102 8364 820
Size: 262 1553
Fragments arranged by size:
1845 1689 1553 1055 926 901 420 262
249 197
SnaBI TAC'GTA
Cuts at: 493 6888 493
Size: 6395 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9097
SphI G_CATG'C
Cuts at: 1132 3313 3364 6455 1132
Size: 2181 51 3091 3774
Fragments arranged by size:
3774 3091 2181 51
SspI AAT'ATT
Cuts at: 5 52 6392 7021 5
Size: 47 6340 629 2081
Fragments arranged by size:
6340 2081 629 47
StuI AGG'CCT
Cuts at: 1517 5668 1517
Size: 4151 4946
StyI C'CwwG_G
Cuts at: 513 1065 2833 3467 4789 4933 5108 5478 6183
Size: 552 1768 634 1322 144 175 370 705
Cuts at: 6183 6282 6536 513
Size: 99 254 3074
Fragments arranged by size:
3074 1768 1322 705 634 552 370 254
175 144 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3529 7124 7141 7294 7479 7924 325
Size: 1255 1949 3595 17 153 185 445 1498
Fragments arranged by size:
3595 1949 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2980 6153 7343
Size: 274 80 33 51 1443 1003 3173 1190
Cuts at: 7343 9055 96
Size: 1712 138
Fragments arranged by size:
3173 1712 1443 1190 1003 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2934 2966 4170 4202 4452 4484 9057 9089 2934
Size: 32 1204 32 250 32 4573 32 2942
Fragments arranged by size:
4573 2942 1204 250 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9097
XbaI T'CTAG_A
Cuts at: 6433 6433
Size: 9097
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 4860 5377 2011
Size: 2849 517 5731
Fragments arranged by size:
5731 2849 517
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9097
XmaI C'CCGG_G
Cuts at: 1870 6422 1870
Size: 4552 4545
XmnI GAAnn'nnTTC
Cuts at: 7226 7226
Size: 9097
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6905 276
Size: 53 83 186 936 5371 2468
Fragments arranged by size:
5371 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SfiI SrfI SwaI