Restriction map for
pFN21AB0120
(Circular) (Six-base) MAPSORT of: pFN21AB0120.seq Check: 4554 from: 1 to: 9292
pFN21AB0120 9292 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6077 6164 6077
Size: 87 9205
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7102 278
Size: 53 83 186 936 5566 2468
Fragments arranged by size:
5566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2856 5087 6635 1937
Size: 919 2231 1548 4594
Fragments arranged by size:
4594 2231 1548 919
Acc65I G'GTAC_C
Cuts at: 6613 6613
Size: 9292
AclI AA'CG_TT
Cuts at: 1563 4998 7419 7792 1563
Size: 3435 2421 373 3063
Fragments arranged by size:
3435 3063 2421 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2240 2333 3212 4378 4548 5501 5511 5546
Size: 608 93 879 1166 170 953 10 35
Cuts at: 5546 7355 8739 9007 1632
Size: 1809 1384 268 1917
Fragments arranged by size:
1917 1809 1384 1166 953 879 608 268
170 93 35 10
AflII C'TTAA_G
Cuts at: 820 1017 4354 820
Size: 197 3337 5758
Fragments arranged by size:
5758 3337 197
AflIII A'CryG_T
Cuts at: 1203 4799 5039 8191 9168 1203
Size: 3596 240 3152 977 1327
Fragments arranged by size:
3596 3152 1327 977 240
AgeI A'CCGG_T
Cuts at: 8123 8123
Size: 9292
AhdI GACnn_n'nnGTC
Cuts at: 8021 8021
Size: 9292
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9292
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2841 6176 6423 1184
Size: 1657 3335 247 4053
Fragments arranged by size:
4053 3335 1657 247
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3191 3223 3191
Size: 32 9260
AlwNI CAG_nnn'CTG
Cuts at: 2029 5134 5376 5684 7035 8607 2029
Size: 3105 242 308 1351 1572 2714
Fragments arranged by size:
3105 2714 1572 1351 308 242
ApaI G_GGCC'C
Cuts at: 2720 6099 2720
Size: 3379 5913
ApaLI G'TGCA_C
Cuts at: 7349 8505 7349
Size: 1156 8136
ApoI r'AATT_y
Cuts at: 1493 2418 2467 2765 3055 3428 3635 4715 5491
Size: 925 49 298 290 373 207 1080 776
Cuts at: 5491 5824 6601 6827 6979 9088 1493
Size: 333 777 226 152 2109 1697
Fragments arranged by size:
2109 1697 1080 925 777 776 373 333
298 290 226 207 152 49
AseI AT'TA_AT
Cuts at: 160 1032 5055 7846 160
Size: 872 4023 2791 1606
Fragments arranged by size:
4023 2791 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9292
AvaI C'yCGr_G
Cuts at: 1870 1943 2107 2688 6617 1870
Size: 73 164 581 3929 4545
Fragments arranged by size:
4545 3929 581 164 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4200 4233 5197 5230 4200
Size: 33 964 33 8262
Fragments arranged by size:
8262 964 33 33
BamHI G'GATC_C
Cuts at: 6622 6989 6622
Size: 367 8925
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2074 3365 5971 6152 6613
Size: 325 217 627 287 1291 2606 181 461
Cuts at: 6613 8068 618
Size: 1455 1842
Fragments arranged by size:
2606 1842 1455 1291 627 461 325 287
217 181
BanII G_rGCy'C
Cuts at: 1391 2524 2720 5272 5407 6099 6611 1391
Size: 1133 196 2552 135 692 512 4072
Fragments arranged by size:
4072 2552 1133 692 512 196 135
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2225 5586 928
Size: 971 326 3361 4634
Fragments arranged by size:
4634 3361 971 326
BbvCI CC'TCA_GC
Cuts at: 6488 6488
Size: 9292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2833 2867 6429 6463 7483
Size: 34 50 34 812 34 3562 34 1020
Cuts at: 7483 7517 1903
Size: 34 3678
Fragments arranged by size:
3678 3562 1020 812 50 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4201 6320 7184 8394 4201
Size: 2119 864 1210 5099
Fragments arranged by size:
5099 2119 1210 864
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9292
BfrBI ATG'CAT
Cuts at: 3280 3280
Size: 9292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6060 7903 136
Size: 107 122 71 631 605 4388 1843 1525
Fragments arranged by size:
4388 1843 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3009 9287 3009
Size: 6278 3014
BlpI GC'TnA_GC
Cuts at: 6709 6709
Size: 9292
Bme1580I G_kGCm'C
Cuts at: 1790 2090 2154 2720 3370 5128 6031 6099 7353
Size: 300 64 566 650 1758 903 68 1254
Cuts at: 7353 8509 1790
Size: 1156 2573
Fragments arranged by size:
2573 1758 1254 1156 903 650 566 300
68 64
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 9292
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3595 4630 7023
Size: 416 53 83 399 24 2178 1035 2393
Cuts at: 7023 7981 442
Size: 958 1753
Fragments arranged by size:
2393 2178 1753 1035 958 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 3329 1056
Size: 2273 7019
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6233 6233
Size: 9292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2976 4692 7952 1239
Size: 1737 1716 3260 2579
Fragments arranged by size:
3260 2579 1737 1716
Bpu10I CC'TnA_GC
Cuts at: 2996 6488 2996
Size: 3492 5800
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3302 7417 8282 8580 3302
Size: 4115 865 298 4014
Fragments arranged by size:
4115 4014 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2879 2897 3350 7955 882
Size: 617 1380 18 453 4605 2219
Fragments arranged by size:
4605 2219 1380 617 453 18
BsaAI yAC'GTr
Cuts at: 493 1204 3686 5209 5301 7083 493
Size: 711 2482 1523 92 1782 2702
Fragments arranged by size:
2702 2482 1782 1523 711 92
BsaBI GATnn'nnATC
Cuts at: 9286 9286
Size: 9292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7099 7481 8116 275
Size: 53 83 186 936 5566 382 635 1451
Fragments arranged by size:
5566 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5928 7725 8123 8397 8544 8955 5928
Size: 1797 398 274 147 411 6265
Fragments arranged by size:
6265 1797 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3191 3221 6224 6254 6518
Size: 30 165 30 1788 30 3003 30 264
Cuts at: 6518 6548 1178
Size: 30 3922
Fragments arranged by size:
3922 3003 1788 264 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2307 3740 4403 4446 5120 6245 6505 6523
Size: 1121 1433 663 43 674 1125 260 18
Cuts at: 6523 1186
Size: 3955
Fragments arranged by size:
3955 1433 1125 1121 674 663 260 43
18
BseYI C'CCAG_C
Cuts at: 2041 4407 6047 8495 8991 2041
Size: 2366 1640 2448 496 2342
Fragments arranged by size:
2448 2366 2342 1640 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3815 5179 5260 6301 6469 6483 1163
Size: 573 2079 1364 81 1041 168 14 3972
Fragments arranged by size:
3972 2079 1364 1041 573 168 81 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6742 7503 8531 1540
Size: 275 186 4741 761 1028 2301
Fragments arranged by size:
4741 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2102 2544 3594 5407 7353 7438 8509 2102
Size: 442 1050 1813 1946 85 1071 2885
Fragments arranged by size:
2885 1946 1813 1071 1050 442 85
BsmI GAATG_Cn'
Cuts at: 2297 2513 2623 3148 6320 6524 6803 6896 2297
Size: 216 110 525 3172 204 279 93 4693
Fragments arranged by size:
4693 3172 525 279 216 204 110 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3626 3626
Size: 9292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2090 2102 2154 2524 2544 2720 3370
Size: 399 300 12 52 370 20 176 650
Cuts at: 3370 3594 5128 5272 5407 6031 6099 6611 7353
Size: 224 1534 144 135 624 68 512 742
Cuts at: 7353 7438 8509 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1534 1071 742 650 624 512 399
370 300 224 176 144 135 85 68
52 20 12
BspEI T'CCGG_A
Cuts at: 5928 8955 5928
Size: 3027 6265
BspHI T'CATG_A
Cuts at: 7181 8911 7181
Size: 1730 7562
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6077 6164 6647 844
Size: 5233 87 483 3489
Fragments arranged by size:
5233 3489 483 87
BsrBI CCG'CTC
Cuts at: 1394 1823 7179 1394
Size: 429 5356 3507
Fragments arranged by size:
5356 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7787 7961 65
Size: 1183 6539 174 1396
Fragments arranged by size:
6539 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5666 7936 8123 1700
Size: 110 3856 2270 187 2869
Fragments arranged by size:
3856 2869 2270 187 110
BsrGI T'GTAC_A
Cuts at: 96 2700 96
Size: 2604 6688
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9292
BssSI C'ACGA_G
Cuts at: 7352 8364 7352
Size: 1012 8280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3029 794
Size: 2235 7057
BstBI TT'CG_AA
Cuts at: 6516 8096 6516
Size: 1580 7712
BstEII G'GTnAC_C
Cuts at: 1184 2780 1184
Size: 1596 7696
BstXI CCAn_nnnn'nTGG
Cuts at: 5894 5894
Size: 9292
BstYI r'GATC_y
Cuts at: 1972 2305 2883 3009 3184 3511 6235 6622 6989
Size: 333 578 126 175 327 2724 387 367
Cuts at: 6989 7374 7391 8843 8929 8941 9287 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
2724 1977 1452 578 387 385 367 346
333 327 175 126 86 17 12
BstZ17I GTA'TAC
Cuts at: 5088 5088
Size: 9292
BtgI C'CryG_G
Cuts at: 513 1065 2446 5352 5769 5917 6031 6205 513
Size: 552 1381 2906 417 148 114 174 3600
Fragments arranged by size:
3600 2906 1381 552 417 174 148 114
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3220 487
Size: 683 56 1994 6559
Fragments arranged by size:
6559 1994 683 56
BtsI GCAGTG_nn'
Cuts at: 2102 2782 2818 3606 4613 5218 5272 5292 6328
Size: 680 36 788 1007 605 54 20 1036
Cuts at: 6328 6479 6808 7602 7622 2102
Size: 151 329 794 20 3772
Fragments arranged by size:
3772 1036 1007 794 788 680 605 329
151 54 36 20 20
ClaI AT'CG_AT
Cuts at: 1554 3481 5229 1554
Size: 1927 1748 5617
Fragments arranged by size:
5617 1927 1748
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9257
DraI TTT'AAA
Cuts at: 2190 6596 2190
Size: 4406 4886
DraIII CAC_nnn'GTG
Cuts at: 2709 5731 2709
Size: 3022 6270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4868 8299 809
Size: 507 3552 3431 1802
Fragments arranged by size:
3552 3431 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3990 4119 6739
Size: 54 1425 50 275 18 2160 129 2620
Cuts at: 6739 7628 9257 8
Size: 889 1629 43
Fragments arranged by size:
2620 2160 1629 1425 889 275 129 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6739 1537
Size: 275 4927 4090
Fragments arranged by size:
4927 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3264 4043 4488 5858 6222 7222 1353
Size: 1911 779 445 1370 364 1000 3423
Fragments arranged by size:
3423 1911 1370 1000 779 445 364
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6517 7879 8253 8399 387
Size: 1064 138 4928 1362 374 146 1280
Fragments arranged by size:
4928 1362 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5405 5405
Size: 9292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2240 2333 2976 3212 4378 4548 4692
Size: 393 608 93 643 236 1166 170 144
Cuts at: 4692 5501 5511 5546 7355 7952 8739 9007 1239
Size: 809 10 35 1809 597 787 268 1524
Fragments arranged by size:
1809 1524 1166 809 787 643 608 597
393 268 236 170 144 93 35 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 5600 2066
Size: 3534 5758
EcoO109I rG'GnC_Cy
Cuts at: 2530 2717 3246 4730 5131 5890 6095 6096 2530
Size: 187 529 1484 401 759 205 1 5726
Fragments arranged by size:
5726 1484 759 529 401 205 187 1
EcoRI G'AATT_C
Cuts at: 3055 6601 6979 3055
Size: 3546 378 5368
Fragments arranged by size:
5368 3546 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2457 3265 5134 2457
Size: 808 1869 6615
Fragments arranged by size:
6615 1869 808
FspI TGC'GCA
Cuts at: 2144 7798 2144
Size: 5654 3638
HaeII r_GCGC'y
Cuts at: 1678 2141 8439 1678
Size: 463 6298 2531
Fragments arranged by size:
6298 2531 463
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2754 2786 2989 3021 3467
Size: 32 135 32 1182 32 203 32 446
Cuts at: 3467 3499 3835 3867 5654 5686 6518 6550 7933
Size: 32 336 32 1787 32 832 32 1383
Cuts at: 7933 7965 8007 8039 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1787 1383 1182 832 446 336 203
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2458 4819 4996 6636 6882 669
Size: 988 281 520 2361 177 1640 246 3079
Fragments arranged by size:
3079 2361 1640 988 520 281 246 177
HindIII A'AGCT_T
Cuts at: 748 2439 2978 3224 748
Size: 1691 539 246 6816
Fragments arranged by size:
6816 1691 539 246
HpaI GTT'AAC
Cuts at: 6882 6882
Size: 9292
KpnI G_GTAC'C
Cuts at: 6617 6617
Size: 9292
MfeI C'AATT_G
Cuts at: 4569 6891 4569
Size: 2322 6970
MluI A'CGCG_T
Cuts at: 9168 9168
Size: 9292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2691 3627 4350 4611 4641 8406 8590 1305
Size: 1386 936 723 261 30 3765 184 2007
Fragments arranged by size:
3765 2007 1386 936 723 261 184 30
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3992 4121 10
Size: 54 1425 343 2160 129 5181
Fragments arranged by size:
5181 2160 1425 343 129 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4506 6210 7250 7609 7768 518
Size: 648 3340 1704 1040 359 159 2042
Fragments arranged by size:
3340 2042 1704 1040 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2353 2496 5586 6186 6342 6487 6708 7385
Size: 747 143 3090 600 156 145 221 677
Cuts at: 7385 8533 8778 1606
Size: 1148 245 2120
Fragments arranged by size:
3090 2120 1148 747 677 600 245 221
156 145 143
NcoI C'CATG_G
Cuts at: 513 1065 5352 6031 6205 513
Size: 552 4287 679 174 3600
Fragments arranged by size:
4287 3600 679 552 174
NdeI CA'TA_TG
Cuts at: 387 2595 4843 5306 387
Size: 2208 2248 463 4373
Fragments arranged by size:
4373 2248 2208 463
NheI G'CTAG_C
Cuts at: 1052 3325 1052
Size: 2273 7019
NotI GC'GGCC_GC
Cuts at: 6739 6739
Size: 9292
NsiI A_TGCA'T
Cuts at: 3282 3282
Size: 9292
NspI r_CATG'y
Cuts at: 1132 1229 4606 4803 5043 5070 5857 6042 6650
Size: 97 3377 197 240 27 787 185 608
Cuts at: 6650 8195 1132
Size: 1545 2229
Fragments arranged by size:
3377 2229 1545 787 608 240 197 185
97 27
PasI CC'CwG_GG
Cuts at: 1348 5894 1348
Size: 4546 4746
PciI A'CATG_T
Cuts at: 4799 5039 8191 4799
Size: 240 3152 5900
Fragments arranged by size:
5900 3152 240
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4117 5731 1108
Size: 3009 1614 4669
Fragments arranged by size:
4669 3009 1614
PfoI T'CCnGG_A
Cuts at: 2301 2526 2953 3585 4110 4669 2301
Size: 225 427 632 525 559 6924
Fragments arranged by size:
6924 632 559 525 427 225
PmeI GTTT'AAAC
Cuts at: 6596 6596
Size: 9292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3191 3223 7361 7393 8874 8906 3191
Size: 32 4138 32 1481 32 3577
Fragments arranged by size:
4138 3577 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2530 3246 4730 5131 2530
Size: 716 1484 401 6691
Fragments arranged by size:
6691 1484 716 401
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9292
PsiI TTA'TAA
Cuts at: 5498 6862 5498
Size: 1364 7928
PspOMI G'GGCC_C
Cuts at: 2716 6095 2716
Size: 3379 5913
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2736 2768 2736
Size: 32 9260
PstI C_TGCA'G
Cuts at: 830 2777 3613 5958 6644 830
Size: 1947 836 2345 686 3478
Fragments arranged by size:
3478 2345 1947 836 686
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9292
PvuII CAG'CTG
Cuts at: 2353 2496 6186 6342 2353
Size: 143 3690 156 5303
Fragments arranged by size:
5303 3690 156 143
SacI G_AGCT'C
Cuts at: 5407 5407
Size: 9292
SalI G'TCGA_C
Cuts at: 1936 6634 1936
Size: 4698 4594
SbfI CC_TGCA'GG
Cuts at: 6644 6644
Size: 9292
ScaI AGT'ACT
Cuts at: 7540 9252 7540
Size: 1712 7580
SfcI C'TryA_G
Cuts at: 826 1045 2773 2839 3609 3933 5954 6181 6640
Size: 219 1728 66 770 324 2021 227 459
Cuts at: 6640 7775 8456 8647 826
Size: 1135 681 191 1471
Fragments arranged by size:
2021 1728 1471 1135 770 681 459 324
227 219 191 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9292
SmaI CCC'GGG
Cuts at: 1872 6619 1872
Size: 4747 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3317 4354 7396 8297 8559 820
Size: 197 926 1374 1037 3042 901 262 1553
Fragments arranged by size:
3042 1553 1374 1037 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3686 5209 7083 493
Size: 3193 1523 1874 2702
Fragments arranged by size:
3193 2702 1874 1523
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9292
SphI G_CATG'C
Cuts at: 1132 5857 6650 1132
Size: 4725 793 3774
Fragments arranged by size:
4725 3774 793
SspI AAT'ATT
Cuts at: 5 52 4747 7216 5
Size: 47 4695 2469 2081
Fragments arranged by size:
4695 2469 2081 47
StuI AGG'CCT
Cuts at: 1517 2686 1517
Size: 1169 8123
StyI C'CwwG_G
Cuts at: 513 1065 2432 3037 3240 4314 5352 5532 5745
Size: 552 1367 605 203 1074 1038 180 213
Cuts at: 5745 6031 6130 6205 6731 513
Size: 286 99 75 526 3074
Fragments arranged by size:
3074 1367 1074 1038 605 552 526 286
213 203 180 99 75
SwaI ATTT'AAAT
Cuts at: 2190 2190
Size: 9292
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4612 5365 6447 7319 7336 7489 7674
Size: 1255 3032 753 1082 872 17 153 185
Cuts at: 7674 8119 325
Size: 445 1498
Fragments arranged by size:
3032 1498 1255 1082 872 753 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2700 3603 4196
Size: 274 80 33 51 1443 723 903 593
Cuts at: 4196 7538 9250 96
Size: 3342 1712 138
Fragments arranged by size:
3342 1712 1443 903 723 593 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2881 2913 4051 4083 5340 5372 9252 9284 2881
Size: 32 1138 32 1257 32 3880 32 2889
Fragments arranged by size:
3880 2889 1257 1138 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9292
XbaI T'CTAG_A
Cuts at: 6628 6628
Size: 9292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4114 5695 4114
Size: 1581 7711
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9292
XmaI C'CCGG_G
Cuts at: 1870 6617 1870
Size: 4747 4545
XmnI GAAnn'nnTTC
Cuts at: 2441 3765 7421 2441
Size: 1324 3656 4312
Fragments arranged by size:
4312 3656 1324
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7100 276
Size: 53 83 186 936 5566 2468
Fragments arranged by size:
5566 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BsiWI Bsu36I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI