Restriction map for
pFN21AB0098
(Circular) (Six-base) MAPSORT of: pFN21AB0098.seq Check: 8917 from: 1 to: 7702
pFN21AB0098 7702 bp
With 182 enzymes: SgfI *
March 28, 2012 14:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5512 278
Size: 53 83 186 936 3976 2468
Fragments arranged by size:
3976 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4332 5045 1937
Size: 2395 713 4594
Fragments arranged by size:
4594 2395 713
Acc65I G'GTAC_C
Cuts at: 2178 5023 2178
Size: 2845 4857
AclI AA'CG_TT
Cuts at: 1563 5829 6202 1563
Size: 4266 373 3063
Fragments arranged by size:
4266 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2467 2767 2889 3162 3403 3919 5765 7149
Size: 835 300 122 273 241 516 1846 1384
Cuts at: 7149 7417 1632
Size: 268 1917
Fragments arranged by size:
1917 1846 1384 835 516 300 273 268
241 122
AflII C'TTAA_G
Cuts at: 820 1017 3821 820
Size: 197 2804 4701
Fragments arranged by size:
4701 2804 197
AflIII A'CryG_T
Cuts at: 1203 3276 6601 7578 1203
Size: 2073 3325 977 1327
Fragments arranged by size:
3325 2073 1327 977
AgeI A'CCGG_T
Cuts at: 6533 6533
Size: 7702
AhdI GACnn_n'nnGTC
Cuts at: 2515 6431 2515
Size: 3916 3786
AleI CACnn'nnGTG
Cuts at: 1166 2172 1166
Size: 1006 6696
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7702
AlwNI CAG_nnn'CTG
Cuts at: 3506 4636 5445 7017 3506
Size: 1130 809 1572 4191
Fragments arranged by size:
4191 1572 1130 809
ApaI G_GGCC'C
Cuts at: 2672 3417 2672
Size: 745 6957
ApaLI G'TGCA_C
Cuts at: 3373 5759 6915 3373
Size: 2386 1156 4160
Fragments arranged by size:
4160 2386 1156
ApoI r'AATT_y
Cuts at: 1493 2339 3777 4156 5011 5237 5389 7498 1493
Size: 846 1438 379 855 226 152 2109 1697
Fragments arranged by size:
2109 1697 1438 855 846 379 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6256 160
Size: 872 5224 1606
Fragments arranged by size:
5224 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
AvaI C'yCGr_G
Cuts at: 1870 1943 2110 5027 1870
Size: 73 167 2917 4545
Fragments arranged by size:
4545 2917 167 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3017 3050 3017
Size: 33 7669
BamHI G'GATC_C
Cuts at: 5032 5399 5032
Size: 367 7335
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2178 3487 5023 6478 618
Size: 325 217 627 391 1309 1536 1455 1842
Fragments arranged by size:
1842 1536 1455 1309 627 391 325 217
BanII G_rGCy'C
Cuts at: 1391 2672 2682 3119 3151 3309 3343 3417 3986
Size: 1281 10 437 32 158 34 74 569
Cuts at: 3986 4646 4667 5021 1391
Size: 660 21 354 4072
Fragments arranged by size:
4072 1281 660 569 437 354 158 74
34 32 21 10
BbeI G_GCGC'C
Cuts at: 3491 3491
Size: 7702
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2224 2773 3866 4479 928
Size: 971 325 549 1093 613 4151
Fragments arranged by size:
4151 1093 971 613 549 325
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4909 4943 5893 5927 1903
Size: 34 2972 34 950 34 3678
Fragments arranged by size:
3678 2972 950 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2927 5594 6804 2927
Size: 2667 1210 3825
Fragments arranged by size:
3825 2667 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7702
BfrBI ATG'CAT
Cuts at: 3614 3614
Size: 7702
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3330 6313 136
Size: 107 122 71 631 605 1658 2983 1525
Fragments arranged by size:
2983 1658 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4822 7697 4822
Size: 2875 4827
BlpI GC'TnA_GC
Cuts at: 5119 5119
Size: 7702
Bme1580I G_kGCm'C
Cuts at: 1790 2425 2672 3377 3417 5763 6919 1790
Size: 635 247 705 40 2346 1156 2573
Fragments arranged by size:
2573 2346 1156 705 635 247 40
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7702
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5433 6391 442
Size: 416 53 83 399 24 4016 958 1753
Fragments arranged by size:
4016 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4534 4658 6362 1239
Size: 3295 124 1704 2579
Fragments arranged by size:
3295 2579 1704 124
Bpu10I CC'TnA_GC
Cuts at: 2676 2676
Size: 7702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3972 5827 6692 6990 3972
Size: 1855 865 298 4684
Fragments arranged by size:
4684 1855 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4909 6365 882
Size: 617 3410 1456 2219
Fragments arranged by size:
3410 2219 1456 617
BsaAI yAC'GTr
Cuts at: 493 1204 3277 3372 5493 493
Size: 711 2073 95 2121 2702
Fragments arranged by size:
2702 2121 2073 711 95
BsaBI GATnn'nnATC
Cuts at: 2840 7696 2840
Size: 4856 2846
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3488 5509 5891 6526
Size: 53 83 186 936 1955 2021 382 635
Cuts at: 6526 275
Size: 1451
Fragments arranged by size:
2021 1955 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3264 6135 6533 6807 6954 7365 3264
Size: 2871 398 274 147 411 3601
Fragments arranged by size:
3601 2871 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3240 3270 3587 3617 4660
Size: 30 165 30 1837 30 317 30 1043
Cuts at: 4660 4690 1178
Size: 30 4190
Fragments arranged by size:
4190 1837 1043 317 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3638 3914 4966 1186
Size: 2452 276 1052 3922
Fragments arranged by size:
3922 2452 1052 276
BseYI C'CCAG_C
Cuts at: 2032 2208 6905 7401 2032
Size: 176 4697 496 2333
Fragments arranged by size:
4697 2333 496 176
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2519 2852 2877 3309 3432 4705 1163
Size: 573 783 333 25 432 123 1273 4160
Fragments arranged by size:
4160 1273 783 573 432 333 123 25
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5152 5913 6941 1540
Size: 275 186 3151 761 1028 2301
Fragments arranged by size:
3151 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3309 3343 3377 3513 3986 4646 5763 5848 6919
Size: 34 34 136 473 660 1117 85 1071
Cuts at: 6919 3309
Size: 4092
Fragments arranged by size:
4092 1117 1071 660 473 136 85 34
34
BsmI GAATG_Cn'
Cuts at: 2901 2958 3614 3616 5213 5306 2901
Size: 57 656 2 1597 93 5297
Fragments arranged by size:
5297 1597 656 93 57 2
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2425 2672 2682 3119 3151 3309 3343
Size: 399 635 247 10 437 32 158 34
Cuts at: 3343 3377 3417 3513 3986 4646 4667 5021 5763
Size: 34 40 96 473 660 21 354 742
Cuts at: 5763 5848 6919 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 660 635 473 437 399
354 247 158 96 85 40 34 34
32 21 10
BspEI T'CCGG_A
Cuts at: 7365 7365
Size: 7702
BspHI T'CATG_A
Cuts at: 3000 4023 4762 4777 5591 7321 3000
Size: 1023 739 15 814 1730 3381
Fragments arranged by size:
3381 1730 1023 814 739 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4047 5057 844
Size: 3203 1010 3489
Fragments arranged by size:
3489 3203 1010
BsrBI CCG'CTC
Cuts at: 1394 1823 5589 1394
Size: 429 3766 3507
Fragments arranged by size:
3766 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4278 4943 6197 6371 65
Size: 1183 3030 665 1254 174 1396
Fragments arranged by size:
3030 1396 1254 1183 665 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2011 6346 6533 1700
Size: 110 201 4335 187 2869
Fragments arranged by size:
4335 2869 201 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7702
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7702
BssSI C'ACGA_G
Cuts at: 5762 6774 5762
Size: 1012 6690
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2429 794
Size: 1635 6067
BstBI TT'CG_AA
Cuts at: 6506 6506
Size: 7702
BstEII G'GTnAC_C
Cuts at: 1184 2251 1184
Size: 1067 6635
BstXI CCAn_nnnn'nTGG
Cuts at: 3871 4744 3871
Size: 873 6829
BstYI r'GATC_y
Cuts at: 1972 3345 3502 4822 5032 5399 5784 5801 7253
Size: 1373 157 1320 210 367 385 17 1452
Cuts at: 7253 7339 7351 7697 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1373 1320 385 367 346 210
157 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 4005 4447 513
Size: 552 939 2001 442 3768
Fragments arranged by size:
3768 2001 939 552 442
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6963
Fragments arranged by size:
6963 683 56
BtsI GCAGTG_nn'
Cuts at: 2889 3117 3861 4813 5218 6012 6032 2889
Size: 228 744 952 405 794 20 4559
Fragments arranged by size:
4559 952 794 744 405 228 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7702
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4668 4703 529
Size: 35 4104 35 3528
Fragments arranged by size:
4104 3528 35 35
DraI TTT'AAA
Cuts at: 3168 5006 3168
Size: 1838 5864
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4211 6709 809
Size: 507 2895 2498 1802
Fragments arranged by size:
2895 2498 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3322 3573 4450
Size: 54 1425 50 275 18 1492 251 877
Cuts at: 4450 5149 6038 7667 8
Size: 699 889 1629 43
Fragments arranged by size:
1629 1492 1425 889 877 699 275 251
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5149 1537
Size: 275 3337 4090
Fragments arranged by size:
4090 3337 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2045 2544 3481 3886 5632 1353
Size: 692 499 937 405 1746 3423
Fragments arranged by size:
3423 1746 937 692 499 405
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6289 6663 6809 387
Size: 1064 138 4700 374 146 1280
Fragments arranged by size:
4700 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3307 3341 3984 4644 3307
Size: 34 643 660 6365
Fragments arranged by size:
6365 660 643 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2467 2767 2889 3162 3403 3919 4534
Size: 393 835 300 122 273 241 516 615
Cuts at: 4534 4658 5765 6362 7149 7417 1239
Size: 124 1107 597 787 268 1524
Fragments arranged by size:
1524 1107 835 787 615 597 516 393
300 273 268 241 124 122
EcoNI CCTnn'n_nnAGG
Cuts at: 2121 4658 4779 2121
Size: 2537 121 5044
Fragments arranged by size:
5044 2537 121
EcoO109I rG'GnC_Cy
Cuts at: 2114 3414 3837 4036 4651 2114
Size: 1300 423 199 615 5165
Fragments arranged by size:
5165 1300 615 423 199
EcoRI G'AATT_C
Cuts at: 5011 5389 5011
Size: 378 7324
EcoRV GAT'ATC
Cuts at: 3270 3270
Size: 7702
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3682 3836 3682
Size: 154 7548
FseI GG_CCGG'CC
Cuts at: 2015 2015
Size: 7702
FspI TGC'GCA
Cuts at: 6208 6208
Size: 7702
HaeII r_GCGC'y
Cuts at: 1678 3491 6849 1678
Size: 1813 3358 2531
Fragments arranged by size:
3358 2531 1813
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2501 2533 2543 2575 2820
Size: 32 135 32 929 32 10 32 245
Cuts at: 2820 2852 2921 2953 3211 3243 3330 3362 3785
Size: 32 69 32 258 32 87 32 423
Cuts at: 3785 3817 3824 3856 4390 4422 4507 4539 4688
Size: 32 7 32 534 32 85 32 149
Cuts at: 4688 4720 4776 4808 4961 4993 6343 6375 6417
Size: 32 56 32 153 32 1350 32 42
Cuts at: 6417 6449 1373
Size: 32 2626
Fragments arranged by size:
2626 1350 929 534 423 258 245 153
149 135 87 85 69 56 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
10 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2686 2719 5046 5292 669
Size: 988 281 748 33 2327 246 3079
Fragments arranged by size:
3079 2327 988 748 281 246 33
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7702
HpaI GTT'AAC
Cuts at: 2719 5292 2719
Size: 2573 5129
KasI G'GCGC_C
Cuts at: 3487 3487
Size: 7702
KpnI G_GTAC'C
Cuts at: 2182 5027 2182
Size: 2845 4857
MfeI C'AATT_G
Cuts at: 5301 5301
Size: 7702
MluI A'CGCG_T
Cuts at: 7578 7578
Size: 7702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2284 3254 3852 6816 7000 1305
Size: 979 970 598 2964 184 2007
Fragments arranged by size:
2964 2007 979 970 598 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3324 3575 4452 10
Size: 54 1425 343 1492 251 877 3260
Fragments arranged by size:
3260 1492 1425 877 343 251 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2172 2331 3945 4742 5660 6019 6178
Size: 648 1006 159 1614 797 918 359 159
Cuts at: 6178 518
Size: 2042
Fragments arranged by size:
2042 1614 1006 918 797 648 359 159
159
MspA1I CmG'CkG
Cuts at: 1606 2020 2026 2152 2983 3049 3889 4856 5118
Size: 414 6 126 831 66 840 967 262
Cuts at: 5118 5795 6943 7188 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 967 840 831 677 414 262
245 126 66 6
NaeI GCC'GGC
Cuts at: 2013 2013
Size: 7702
NarI GG'CG_CC
Cuts at: 3488 3488
Size: 7702
NcoI C'CATG_G
Cuts at: 513 1065 2004 4005 4447 513
Size: 552 939 2001 442 3768
Fragments arranged by size:
3768 2001 939 552 442
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7702
NgoMIV G'CCGG_C
Cuts at: 2011 2011
Size: 7702
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7702
NotI GC'GGCC_GC
Cuts at: 5149 5149
Size: 7702
NsiI A_TGCA'T
Cuts at: 3616 3616
Size: 7702
NspI r_CATG'y
Cuts at: 1132 1229 4195 4927 5060 6605 1132
Size: 97 2966 732 133 1545 2229
Fragments arranged by size:
2966 2229 1545 732 133 97
PasI CC'CwG_GG
Cuts at: 1348 4129 4660 1348
Size: 2781 531 4390
Fragments arranged by size:
4390 2781 531
PciI A'CATG_T
Cuts at: 6601 6601
Size: 7702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7702
PfoI T'CCnGG_A
Cuts at: 3591 3591
Size: 7702
PmeI GTTT'AAAC
Cuts at: 5006 5006
Size: 7702
PmlI CAC'GTG
Cuts at: 3277 3277
Size: 7702
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5771 5803 7284 7316 5771
Size: 32 1481 32 6157
Fragments arranged by size:
6157 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2114 3837 4036 2114
Size: 1723 199 5780
Fragments arranged by size:
5780 1723 199
PshAI GACnn'nnGTC
Cuts at: 1613 2258 1613
Size: 645 7057
PsiI TTA'TAA
Cuts at: 5272 5272
Size: 7702
PspOMI G'GGCC_C
Cuts at: 2668 3413 2668
Size: 745 6957
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3678 3710 3678
Size: 32 7670
PstI C_TGCA'G
Cuts at: 830 3112 4098 4563 4849 5054 830
Size: 2282 986 465 286 205 3478
Fragments arranged by size:
3478 2282 986 465 286 205
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7702
PvuII CAG'CTG
Cuts at: 3049 4856 3049
Size: 1807 5895
SacI G_AGCT'C
Cuts at: 3309 3343 3986 4646 3309
Size: 34 643 660 6365
Fragments arranged by size:
6365 660 643 34
SalI G'TCGA_C
Cuts at: 1936 5044 1936
Size: 3108 4594
SbfI CC_TGCA'GG
Cuts at: 5054 5054
Size: 7702
ScaI AGT'ACT
Cuts at: 5950 7662 5950
Size: 1712 5990
SfcI C'TryA_G
Cuts at: 826 1045 3108 4094 4559 4845 5050 6185 6866
Size: 219 2063 986 465 286 205 1135 681
Cuts at: 6866 7057 826
Size: 191 1471
Fragments arranged by size:
2063 1471 1135 986 681 465 286 219
205 191
SfoI GGC'GCC
Cuts at: 3489 3489
Size: 7702
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7702
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7702
SmaI CCC'GGG
Cuts at: 1872 2112 5029 1872
Size: 240 2917 4545
Fragments arranged by size:
4545 2917 240
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3821 3987 5806 6707 6969 820
Size: 197 926 1878 166 1819 901 262 1553
Fragments arranged by size:
1878 1819 1553 926 901 262 197 166
SnaBI TAC'GTA
Cuts at: 493 5493 493
Size: 5000 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7702
SphI G_CATG'C
Cuts at: 1132 4927 5060 1132
Size: 3795 133 3774
Fragments arranged by size:
3795 3774 133
SspI AAT'ATT
Cuts at: 5 52 3438 5626 5
Size: 47 3386 2188 2081
Fragments arranged by size:
3386 2188 2081 47
StuI AGG'CCT
Cuts at: 1517 2763 1517
Size: 1246 6456
StyI C'CwwG_G
Cuts at: 513 1065 2004 2823 3119 3570 4005 4270 4447
Size: 552 939 819 296 451 435 265 177
Cuts at: 4447 4932 5141 513
Size: 485 209 3074
Fragments arranged by size:
3074 939 819 552 485 451 435 296
265 209 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3773 3957 4341 5729 5746 5899 6084
Size: 1255 2193 184 384 1388 17 153 185
Cuts at: 6084 6529 325
Size: 445 1498
Fragments arranged by size:
2193 1498 1388 1255 445 384 185 184
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2581 4503 5948
Size: 274 80 33 51 1443 604 1922 1445
Cuts at: 5948 7660 96
Size: 1712 138
Fragments arranged by size:
1922 1712 1445 1443 604 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3240 3272 4660 4692 7662 7694 3240
Size: 32 1388 32 2970 32 3248
Fragments arranged by size:
3248 2970 1388 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2818 4328 1316
Size: 1502 1510 4690
Fragments arranged by size:
4690 1510 1502
XbaI T'CTAG_A
Cuts at: 5038 5038
Size: 7702
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3307 3307
Size: 7702
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7702
XmaI C'CCGG_G
Cuts at: 1870 2110 5027 1870
Size: 240 2917 4545
Fragments arranged by size:
4545 2917 240
XmnI GAAnn'nnTTC
Cuts at: 3614 3665 3932 5831 3614
Size: 51 267 1899 5485
Fragments arranged by size:
5485 1899 267 51
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5510 276
Size: 53 83 186 936 3976 2468
Fragments arranged by size:
3976 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbvCI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII FspAI NruI PacI PspXI
RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI