Restriction map for
pFN21AB0087
(Circular) (Six-base) MAPSORT of: pFN21AB0087.seq Check: 625 from: 1 to: 8125
pFN21AB0087 8125 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5935 278
Size: 53 83 186 936 4399 2468
Fragments arranged by size:
4399 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2547 5468 1937
Size: 610 2921 4594
Fragments arranged by size:
4594 2921 610
Acc65I G'GTAC_C
Cuts at: 5446 5446
Size: 8125
AclI AA'CG_TT
Cuts at: 1563 3015 6252 6625 1563
Size: 1452 3237 373 3063
Fragments arranged by size:
3237 3063 1452 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2667 3405 4539 4766 6188 7572 7840 1632
Size: 1035 738 1134 227 1422 1384 268 1917
Fragments arranged by size:
1917 1422 1384 1134 1035 738 268 227
AfeI AGC'GCT
Cuts at: 3834 4270 4948 3834
Size: 436 678 7011
Fragments arranged by size:
7011 678 436
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7928
AflIII A'CryG_T
Cuts at: 1203 4038 7024 8001 1203
Size: 2835 2986 977 1327
Fragments arranged by size:
2986 2835 1327 977
AgeI A'CCGG_T
Cuts at: 6956 6956
Size: 8125
AhdI GACnn_n'nnGTC
Cuts at: 3080 6854 3080
Size: 3774 4351
AleI CACnn'nnGTG
Cuts at: 1166 2045 2727 2777 3278 1166
Size: 879 682 50 501 6013
Fragments arranged by size:
6013 879 682 501 50
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2246 1184
Size: 1062 7063
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3913 3945 3913
Size: 32 8093
AlwNI CAG_nnn'CTG
Cuts at: 4132 4285 4426 5053 5868 7440 4132
Size: 153 141 627 815 1572 4817
Fragments arranged by size:
4817 1572 815 627 153 141
ApaI G_GGCC'C
Cuts at: 2621 3550 2621
Size: 929 7196
ApaLI G'TGCA_C
Cuts at: 4367 6182 7338 4367
Size: 1815 1156 5154
Fragments arranged by size:
5154 1815 1156
ApoI r'AATT_y
Cuts at: 1493 2606 4628 5434 5660 5812 7921 1493
Size: 1113 2022 806 226 152 2109 1697
Fragments arranged by size:
2109 2022 1697 1113 806 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6679 160
Size: 872 5647 1606
Fragments arranged by size:
5647 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8125
AvaI C'yCGr_G
Cuts at: 1870 1943 4842 5450 1870
Size: 73 2899 608 4545
Fragments arranged by size:
4545 2899 608 73
BamHI G'GATC_C
Cuts at: 5418 5455 5822 5418
Size: 37 367 7721
Fragments arranged by size:
7721 367 37
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2882 3899 4109 5446 6901
Size: 325 217 627 1095 1017 210 1337 1455
Cuts at: 6901 618
Size: 1842
Fragments arranged by size:
1842 1455 1337 1095 1017 627 325 217
210
BanII G_rGCy'C
Cuts at: 1391 2317 2621 2679 3550 3582 3852 4533 5444
Size: 926 304 58 871 32 270 681 911
Cuts at: 5444 1391
Size: 4072
Fragments arranged by size:
4072 926 911 871 681 304 270 58
32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2091 3997 4754 5252 928
Size: 971 192 1906 757 498 3801
Fragments arranged by size:
3801 1906 971 757 498 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6316 6350 1903
Size: 34 4379 34 3678
Fragments arranged by size:
4379 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4524 6017 7227 4524
Size: 1493 1210 5422
Fragments arranged by size:
5422 1493 1210
BclI T'GATC_A
Cuts at: 1548 3364 1548
Size: 1816 6309
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6736 136
Size: 107 122 71 631 605 5064 1525
Fragments arranged by size:
5064 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2124 4173 8120 2124
Size: 2049 3947 2129
Fragments arranged by size:
3947 2129 2049
BlpI GC'TnA_GC
Cuts at: 5542 5542
Size: 8125
Bme1580I G_kGCm'C
Cuts at: 1790 2621 3550 3902 4250 4371 4502 6186 7342
Size: 831 929 352 348 121 131 1684 1156
Cuts at: 7342 1790
Size: 2573
Fragments arranged by size:
2573 1684 1156 929 831 352 348 131
121
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8125
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4189 5856 6814
Size: 416 53 83 399 24 2772 1667 958
Cuts at: 6814 442
Size: 1753
Fragments arranged by size:
2772 1753 1667 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 4275 1056
Size: 3219 4906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2291 2501 3125 4198 4288 4303 4964 5242
Size: 1052 210 624 1073 90 15 661 278
Cuts at: 5242 6785 1239
Size: 1543 2579
Fragments arranged by size:
2579 1543 1073 1052 661 624 278 210
90 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2444 2794 2879 3429 4007 4279 6250 7115 7413
Size: 350 85 550 578 272 1971 865 298
Cuts at: 7413 2444
Size: 3156
Fragments arranged by size:
3156 1971 865 578 550 350 298 272
85
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2348 2918 2985 3445 4334 6788 882
Size: 617 849 570 67 460 889 2454 2219
Fragments arranged by size:
2454 2219 889 849 617 570 460 67
BsaAI yAC'GTr
Cuts at: 493 1204 2020 2572 5916 493
Size: 711 816 552 3344 2702
Fragments arranged by size:
3344 2702 816 711 552
BsaBI GATnn'nnATC
Cuts at: 4062 8119 4062
Size: 4057 4068
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5932 6314 6949 275
Size: 53 83 186 936 4399 382 635 1451
Fragments arranged by size:
4399 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2063 3174 6558 6956 7230 7377 7788 2063
Size: 1111 3384 398 274 147 411 2400
Fragments arranged by size:
3384 2400 1111 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3315 3345 4344 4374 1178
Size: 30 165 30 1912 30 999 30 4929
Fragments arranged by size:
4929 1912 999 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 3341 4323 4979 5420 1186
Size: 1312 843 982 656 441 3891
Fragments arranged by size:
3891 1312 982 843 656 441
BseYI C'CCAG_C
Cuts at: 2821 2997 3748 3903 4265 4465 7328 7824 2821
Size: 176 751 155 362 200 2863 496 3122
Fragments arranged by size:
3122 2863 751 496 362 200 176 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3719 4118 1163
Size: 573 1983 399 5170
Fragments arranged by size:
5170 1983 573 399
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 3880 5575 6336 7364 1540
Size: 275 186 81 1798 1695 761 1028 2301
Fragments arranged by size:
2301 1798 1695 1028 761 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 3285 3582 4371 5091 5214 6186 6271 7342 3285
Size: 297 789 720 123 972 85 1071 4068
Fragments arranged by size:
4068 1071 972 789 720 297 123 85
BsmI GAATG_Cn'
Cuts at: 5636 5729 5636
Size: 93 8032
BsmBI CGTCTCn'nnnn_
Cuts at: 2411 2411
Size: 8125
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 2621 2679 3285 3550 3582 3852
Size: 399 527 304 58 606 265 32 270
Cuts at: 3852 3902 4250 4371 4502 4533 5091 5214 5444
Size: 50 348 121 131 31 558 123 230
Cuts at: 5444 6186 6271 7342 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 606 558 527 399 348
304 270 265 230 131 123 121 85
58 50 32 31
BspEI T'CCGG_A
Cuts at: 3174 7788 3174
Size: 4614 3511
BspHI T'CATG_A
Cuts at: 4336 6014 7744 4336
Size: 1678 1730 4717
Fragments arranged by size:
4717 1730 1678
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2826 5480 844
Size: 1394 588 2654 3489
Fragments arranged by size:
3489 2654 1394 588
BsrBI CCG'CTC
Cuts at: 1394 1823 4969 5242 6012 1394
Size: 429 3146 273 770 3507
Fragments arranged by size:
3507 3146 770 429 273
BsrDI GCAATG_nn'
Cuts at: 65 1248 2329 3658 4512 6620 6794 65
Size: 1183 1081 1329 854 2108 174 1396
Fragments arranged by size:
2108 1396 1329 1183 1081 854 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2357 2838 6769 6956 1700
Size: 110 547 481 3931 187 2869
Fragments arranged by size:
3931 2869 547 481 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8125
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8125
BssSI C'ACGA_G
Cuts at: 2113 3194 4237 6185 7197 2113
Size: 1081 1043 1948 1012 3041
Fragments arranged by size:
3041 1948 1081 1043 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4710 794
Size: 3916 4209
BstBI TT'CG_AA
Cuts at: 6929 6929
Size: 8125
BstEII G'GTnAC_C
Cuts at: 1184 2134 5122 1184
Size: 950 2988 4187
Fragments arranged by size:
4187 2988 950
BstXI CCAn_nnnn'nTGG
Cuts at: 3460 4988 3460
Size: 1528 6597
BstYI r'GATC_y
Cuts at: 1972 2124 2899 3564 4122 4173 5225 5418 5455
Size: 152 775 665 558 51 1052 193 37
Cuts at: 5455 5822 6207 6224 7676 7762 7774 8120 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1052 775 665 558 385 367
346 193 152 86 51 37 17 12
Bsu36I CC'TnA_GG
Cuts at: 2559 3483 3573 2559
Size: 924 90 7111
Fragments arranged by size:
7111 924 90
BtgI C'CryG_G
Cuts at: 513 1065 2317 2655 3051 4815 5029 513
Size: 552 1252 338 396 1764 214 3609
Fragments arranged by size:
3609 1764 1252 552 396 338 214
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7386
Fragments arranged by size:
7386 683 56
BtsI GCAGTG_nn'
Cuts at: 3239 4300 4326 5376 5641 6435 6455 3239
Size: 1061 26 1050 265 794 20 4909
Fragments arranged by size:
4909 1061 1050 794 265 26 20
ClaI AT'CG_AT
Cuts at: 1554 4116 1554
Size: 2562 5563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8090
DraI TTT'AAA
Cuts at: 5429 5429
Size: 8125
DraIII CAC_nnn'GTG
Cuts at: 4710 4710
Size: 8125
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5131 7132 809
Size: 507 3815 2001 1802
Fragments arranged by size:
3815 2001 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2341 3048
Size: 54 1425 50 275 18 249 262 707
Cuts at: 3048 3236 4083 5572 6461 8090 8
Size: 188 847 1489 889 1629 43
Fragments arranged by size:
1629 1489 1425 889 847 707 275 262
249 188 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2079 5572 1537
Size: 275 267 3493 4090
Fragments arranged by size:
4090 3493 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 2849 4350 4515 4827 5096 6055 1353
Size: 1496 1501 165 312 269 959 3423
Fragments arranged by size:
3423 1501 1496 959 312 269 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2465 6712 7086 7232 387
Size: 1064 138 876 4247 374 146 1280
Fragments arranged by size:
4247 1280 1064 876 374 146 138
EcoICRI GAG'CTC
Cuts at: 3580 3580
Size: 8125
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2291 2501 2667 3125 3405 4198 4288
Size: 393 659 210 166 458 280 793 90
Cuts at: 4288 4303 4539 4766 4964 5242 6188 6785 7572
Size: 15 236 227 198 278 946 597 787
Cuts at: 7572 7840 1239
Size: 268 1524
Fragments arranged by size:
1524 946 793 787 659 597 458 393
280 278 268 236 227 210 198 166
90 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2423 4982 5393 2423
Size: 2559 411 5155
Fragments arranged by size:
5155 2559 411
EcoO109I rG'GnC_Cy
Cuts at: 2227 2554 2617 2618 3074 3546 3547 4207 2227
Size: 327 63 1 456 472 1 660 6145
Fragments arranged by size:
6145 660 472 456 327 63 1 1
EcoRI G'AATT_C
Cuts at: 2606 5434 5812 2606
Size: 2828 378 4919
Fragments arranged by size:
4919 2828 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4777 4777
Size: 8125
FspI TGC'GCA
Cuts at: 4703 6631 4703
Size: 1928 6197
HaeII r_GCGC'y
Cuts at: 1678 3836 4082 4272 4950 7272 1678
Size: 2158 246 190 678 2322 2531
Fragments arranged by size:
2531 2322 2158 678 246 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2109 2141 3066 3098 3556
Size: 32 135 32 537 32 925 32 458
Cuts at: 3556 3588 3751 3783 6766 6798 6840 6872 1373
Size: 32 163 32 2983 32 42 32 2626
Fragments arranged by size:
2983 2626 925 537 458 163 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3019 4018 5104 5469 5715 669
Size: 988 281 1081 999 1086 365 246 3079
Fragments arranged by size:
3079 1086 1081 999 988 365 281 246
HindIII A'AGCT_T
Cuts at: 748 2649 748
Size: 1901 6224
HpaI GTT'AAC
Cuts at: 5715 5715
Size: 8125
KpnI G_GTAC'C
Cuts at: 5450 5450
Size: 8125
MfeI C'AATT_G
Cuts at: 5724 5724
Size: 8125
MluI A'CGCG_T
Cuts at: 8001 8001
Size: 8125
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7239 7423 1305
Size: 5934 184 2007
Fragments arranged by size:
5934 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3050 3238 4085 10
Size: 54 1425 343 1218 188 847 4050
Fragments arranged by size:
4050 1425 1218 847 343 188 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2045 2322 2727 2777 3198 3278 3500
Size: 648 879 277 405 50 421 80 222
Cuts at: 3500 3602 3779 4505 4769 6083 6442 6601 518
Size: 102 177 726 264 1314 359 159 2042
Fragments arranged by size:
2042 1314 879 726 648 421 405 359
277 264 222 177 159 102 80 50
MspA1I CmG'CkG
Cuts at: 1606 3127 5358 5541 6218 7366 7611 1606
Size: 1521 2231 183 677 1148 245 2120
Fragments arranged by size:
2231 2120 1521 1148 677 245 183
NaeI GCC'GGC
Cuts at: 2840 2840
Size: 8125
NcoI C'CATG_G
Cuts at: 513 1065 2317 2655 3051 4815 513
Size: 552 1252 338 396 1764 3823
Fragments arranged by size:
3823 1764 1252 552 396 338
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8125
NgoMIV G'CCGG_C
Cuts at: 2838 2838
Size: 8125
NheI G'CTAG_C
Cuts at: 1052 4271 1052
Size: 3219 4906
NotI GC'GGCC_GC
Cuts at: 5572 5572
Size: 8125
NruI TCG'CGA
Cuts at: 2445 2445
Size: 8125
NspI r_CATG'y
Cuts at: 1132 1229 2176 2495 3203 4042 5167 5483 7028
Size: 97 947 319 708 839 1125 316 1545
Cuts at: 7028 1132
Size: 2229
Fragments arranged by size:
2229 1545 1125 947 839 708 319 316
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8125
PciI A'CATG_T
Cuts at: 4038 7024 4038
Size: 2986 5139
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3529 4000 1108
Size: 2421 471 5233
Fragments arranged by size:
5233 2421 471
PfoI T'CCnGG_A
Cuts at: 2268 2328 4980 5295 2268
Size: 60 2652 315 5098
Fragments arranged by size:
5098 2652 315 60
PmeI GTTT'AAAC
Cuts at: 5429 5429
Size: 8125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6194 6226 7707 7739 6194
Size: 32 1481 32 6580
Fragments arranged by size:
6580 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2227 2554 3074 4207 2227
Size: 327 520 1133 6145
Fragments arranged by size:
6145 1133 520 327
PshAI GACnn'nnGTC
Cuts at: 1613 2707 1613
Size: 1094 7031
PsiI TTA'TAA
Cuts at: 5695 5695
Size: 8125
PspOMI G'GGCC_C
Cuts at: 2617 3546 2617
Size: 929 7196
PstI C_TGCA'G
Cuts at: 830 3564 4431 5383 5477 830
Size: 2734 867 952 94 3478
Fragments arranged by size:
3478 2734 952 867 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8125
PvuII CAG'CTG
Cuts at: 3127 3127
Size: 8125
SacI G_AGCT'C
Cuts at: 3582 3582
Size: 8125
SalI G'TCGA_C
Cuts at: 1936 5467 1936
Size: 3531 4594
SapI GCTCTTCn'nnn_
Cuts at: 2849 5096 2849
Size: 2247 5878
SbfI CC_TGCA'GG
Cuts at: 5477 5477
Size: 8125
ScaI AGT'ACT
Cuts at: 6373 8085 6373
Size: 1712 6413
SfcI C'TryA_G
Cuts at: 826 1045 3537 3560 3982 4427 4577 5339 5379
Size: 219 2492 23 422 445 150 762 40
Cuts at: 5379 5473 6608 7289 7480 826
Size: 94 1135 681 191 1471
Fragments arranged by size:
2492 1471 1135 762 681 445 422 219
191 150 94 40 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8125
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8125
SmaI CCC'GGG
Cuts at: 1872 5452 1872
Size: 3580 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2459 2809 2894 3444 4022 4258
Size: 197 926 516 350 85 550 578 236
Cuts at: 4258 4842 6229 7130 7392 820
Size: 584 1387 901 262 1553
Fragments arranged by size:
1553 1387 926 901 584 578 550 516
350 262 236 197 85
SnaBI TAC'GTA
Cuts at: 493 5916 493
Size: 5423 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8125
SphI G_CATG'C
Cuts at: 1132 3203 5483 1132
Size: 2071 2280 3774
Fragments arranged by size:
3774 2280 2071
SspI AAT'ATT
Cuts at: 5 52 6049 5
Size: 47 5997 2081
Fragments arranged by size:
5997 2081 47
StuI AGG'CCT
Cuts at: 1517 2465 1517
Size: 948 7177
StyI C'CwwG_G
Cuts at: 513 1065 2211 2317 2621 2655 2917 3051 3819
Size: 552 1146 106 304 34 262 134 768
Cuts at: 3819 4166 4752 4815 5564 513
Size: 347 586 63 749 3074
Fragments arranged by size:
3074 1146 768 749 586 552 347 304
262 134 106 63 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2978 3740 3917 4292 5045 6152 6169
Size: 1255 1398 762 177 375 753 1107 17
Cuts at: 6169 6322 6507 6952 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1398 1255 1107 762 753 445 375
185 177 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2253 4614 6371
Size: 274 80 33 51 1443 276 2361 1757
Cuts at: 6371 8083 96
Size: 1712 138
Fragments arranged by size:
2361 1757 1712 1443 276 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4344 4376 8085 8117 4344
Size: 32 3709 32 4352
Fragments arranged by size:
4352 3709 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8125
XbaI T'CTAG_A
Cuts at: 5461 5461
Size: 8125
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2336 4238 2336
Size: 1902 6223
XhoI C'TCGA_G
Cuts at: 1943 4842 1943
Size: 2899 5226
XmaI C'CCGG_G
Cuts at: 1870 5450 1870
Size: 3580 4545
XmnI GAAnn'nnTTC
Cuts at: 4407 6254 4407
Size: 1847 6278
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5933 276
Size: 53 83 186 936 4399 2468
Fragments arranged by size:
4399 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BstZ17I EcoRV FseI FspAI KasI NarI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI