Restriction map for
pFN21AB0069
(Circular) (Six-base) MAPSORT of: pFN21AB0069.seq Check: 7631 from: 1 to: 12445
pFN21AB0069 12445 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2738 2738
Size: 12445
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 10255 278
Size: 53 83 186 936 8719 2468
Fragments arranged by size:
8719 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 9788 1937
Size: 7851 4594
Acc65I G'GTAC_C
Cuts at: 9766 9766
Size: 12445
AclI AA'CG_TT
Cuts at: 1563 10572 10945 1563
Size: 9009 373 3063
Fragments arranged by size:
9009 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2256 3146 3902 4088 4212 4251 4296 4538
Size: 624 890 756 186 124 39 45 242
Cuts at: 4538 4625 6375 6719 7139 8180 8951 10508 11892
Size: 87 1750 344 420 1041 771 1557 1384
Cuts at: 11892 12160 1632
Size: 268 1917
Fragments arranged by size:
1917 1750 1557 1384 1041 890 771 756
624 420 344 268 242 186 124 87
45 39
AflII C'TTAA_G
Cuts at: 820 1017 6821 7403 820
Size: 197 5804 582 5862
Fragments arranged by size:
5862 5804 582 197
AflIII A'CryG_T
Cuts at: 1203 2386 2909 4565 11344 12321 1203
Size: 1183 523 1656 6779 977 1327
Fragments arranged by size:
6779 1656 1327 1183 977 523
AgeI A'CCGG_T
Cuts at: 11276 11276
Size: 12445
AhdI GACnn_n'nnGTC
Cuts at: 11174 11174
Size: 12445
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 12445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 9740 1184
Size: 8556 3889
AlwNI CAG_nnn'CTG
Cuts at: 2744 3410 3808 6277 7237 8635 10188 11760 2744
Size: 666 398 2469 960 1398 1553 1572 3429
Fragments arranged by size:
3429 2469 1572 1553 1398 960 666 398
ApaLI G'TGCA_C
Cuts at: 10502 11658 10502
Size: 1156 11289
ApoI r'AATT_y
Cuts at: 1493 2375 2698 2874 2884 3370 3611 4382 4390
Size: 882 323 176 10 486 241 771 8
Cuts at: 4390 4496 4887 4909 4949 5413 5936 7366 7537
Size: 106 391 22 40 464 523 1430 171
Cuts at: 7537 7543 7767 7788 8027 8135 8219 8364 8411
Size: 6 224 21 239 108 84 145 47
Cuts at: 8411 8787 8803 9071 9306 9451 9529 9754 9980
Size: 376 16 268 235 145 78 225 226
Cuts at: 9980 10132 12241 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 1430 882 771 523 486 464
391 376 323 268 241 239 235 226
225 224 176 171 152 145 145 108
106 84 78 47 40 22 21 16
10 8 6
AseI AT'TA_AT
Cuts at: 160 1032 4869 5400 6408 10999 160
Size: 872 3837 531 1008 4591 1606
Fragments arranged by size:
4591 3837 1606 1008 872 531
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12445
AvaI C'yCGr_G
Cuts at: 1870 1943 6644 9770 1870
Size: 73 4701 3126 4545
Fragments arranged by size:
4701 4545 3126 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2919 2952 9165 9198 2919
Size: 33 6213 33 6166
Fragments arranged by size:
6213 6166 33 33
BamHI G'GATC_C
Cuts at: 9775 10142 9775
Size: 367 12078
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3273 8708 9766 11221 618
Size: 325 217 627 1486 5435 1058 1455 1842
Fragments arranged by size:
5435 1842 1486 1455 1058 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3109 4239 9764 1391
Size: 1718 1130 5525 4072
Fragments arranged by size:
5525 4072 1718 1130
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3699 3789 4529 5144 5717 7334 8696
Size: 971 1800 90 740 615 573 1617 1362
Cuts at: 8696 928
Size: 4677
Fragments arranged by size:
4677 1800 1617 1362 971 740 615 573
90
BbvCI CC'TCA_GC
Cuts at: 4205 9204 4205
Size: 4999 7446
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 10636 10670 1903
Size: 34 8699 34 3678
Fragments arranged by size:
8699 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2698 10337 11547 2698
Size: 7639 1210 3596
Fragments arranged by size:
7639 3596 1210
BclI T'GATC_A
Cuts at: 1548 2808 4375 7975 1548
Size: 1260 1567 3600 6018
Fragments arranged by size:
6018 3600 1567 1260
BfrBI ATG'CAT
Cuts at: 7114 7273 8836 7114
Size: 159 1563 10723
Fragments arranged by size:
10723 1563 159
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 11056 136
Size: 107 122 71 631 605 9384 1525
Fragments arranged by size:
9384 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6974 7684 9016 12440 6974
Size: 710 1332 3424 6979
Fragments arranged by size:
6979 3424 1332 710
BlpI GC'TnA_GC
Cuts at: 8991 9862 8991
Size: 871 11574
Bme1580I G_kGCm'C
Cuts at: 1790 8713 10506 11662 1790
Size: 6923 1793 1156 2573
Fragments arranged by size:
6923 2573 1793 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 12445
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5771 10176 11134
Size: 416 53 83 399 24 4354 4405 958
Cuts at: 11134 442
Size: 1753
Fragments arranged by size:
4405 4354 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12445
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3523 7058 7667 9164 3523
Size: 3535 609 1497 6804
Fragments arranged by size:
6804 3535 1497 609
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2864 2916 2948 3896 4007 5873 7658 11105
Size: 1625 52 32 948 111 1866 1785 3447
Cuts at: 11105 1239
Size: 2579
Fragments arranged by size:
3447 2579 1866 1785 1625 948 111 52
32
Bpu10I CC'TnA_GC
Cuts at: 3108 4205 5921 6252 9204 3108
Size: 1097 1716 331 2952 6349
Fragments arranged by size:
6349 2952 1716 1097 331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2744 4460 4698 4800 4859 5495 6783 7028 7637
Size: 1716 238 102 59 636 1288 245 609
Cuts at: 7637 8894 9616 10570 11435 11733 2744
Size: 1257 722 954 865 298 3456
Fragments arranged by size:
3456 1716 1288 1257 954 865 722 636
609 298 245 238 102 59
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5571 6741 6884 7029 7068 7638 11108
Size: 617 4072 1170 143 145 39 570 3470
Cuts at: 11108 882
Size: 2219
Fragments arranged by size:
4072 3470 2219 1170 617 570 145 143
39
BsaAI yAC'GTr
Cuts at: 493 1204 2387 2912 4568 10236 493
Size: 711 1183 525 1656 5668 2702
Fragments arranged by size:
5668 2702 1656 1183 711 525
BsaBI GATnn'nnATC
Cuts at: 12439 12439
Size: 12445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 10252 10634 11269 275
Size: 53 83 186 936 8719 382 635 1451
Fragments arranged by size:
8719 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 10878 11276 11550 11697 12108 10878
Size: 398 274 147 411 11215
Fragments arranged by size:
11215 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2862 2892 2914 2944 6842
Size: 30 165 30 1459 30 22 30 3898
Cuts at: 6842 6872 1178
Size: 30 6751
Fragments arranged by size:
6751 3898 1459 165 30 30 30 30
30 22
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3098 4457 7028 7106 7637 9700 1186
Size: 1912 1359 2571 78 531 2063 3931
Fragments arranged by size:
3931 2571 2063 1912 1359 531 78
BseYI C'CCAG_C
Cuts at: 11648 12144 11648
Size: 496 11949
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2905 1163
Size: 573 1169 10703
Fragments arranged by size:
10703 1169 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2048 9895 10656 11684 1540
Size: 275 186 47 7847 761 1028 2301
Fragments arranged by size:
7847 2301 1028 761 275 186 47
BsiHKAI G_wGCw'C
Cuts at: 4239 7128 7934 10506 10591 11662 4239
Size: 2889 806 2572 85 1071 5022
Fragments arranged by size:
5022 2889 2572 1071 806 85
BsmI GAATG_Cn'
Cuts at: 9956 10049 9956
Size: 93 12352
BsmBI CGTCTCn'nnnn_
Cuts at: 3092 3092
Size: 12445
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3109 4239 7128 7934 8713 9764 10506
Size: 399 1319 1130 2889 806 779 1051 742
Cuts at: 10506 10591 11662 1391
Size: 85 1071 2174
Fragments arranged by size:
2889 2174 1319 1130 1071 1051 806 779
742 399 85
BspEI T'CCGG_A
Cuts at: 12108 12108
Size: 12445
BspHI T'CATG_A
Cuts at: 2335 5785 6229 7561 8148 8917 10334 12064 2335
Size: 3450 444 1332 587 769 1417 1730 2716
Fragments arranged by size:
3450 2716 1730 1417 1332 769 587 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2738 9800 844
Size: 1894 7062 3489
Fragments arranged by size:
7062 3489 1894
BsrBI CCG'CTC
Cuts at: 1394 1823 10332 1394
Size: 429 8509 3507
Fragments arranged by size:
8509 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3120 10940 11114 65
Size: 1183 1872 7820 174 1396
Fragments arranged by size:
7820 1872 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 11089 11276 1700
Size: 110 9279 187 2869
Fragments arranged by size:
9279 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5365 9250 96
Size: 5269 3885 3291
Fragments arranged by size:
5269 3885 3291
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12445
BssSI C'ACGA_G
Cuts at: 10505 11517 10505
Size: 1012 11433
BstAPI GCAn_nnn'nTGC
Cuts at: 794 8708 794
Size: 7914 4531
BstBI TT'CG_AA
Cuts at: 11249 11249
Size: 12445
BstEII G'GTnAC_C
Cuts at: 1184 3208 1184
Size: 2024 10421
BstXI CCAn_nnnn'nTGG
Cuts at: 9699 9699
Size: 12445
BstYI r'GATC_y
Cuts at: 1972 2675 3064 5772 6501 6974 7234 7684 8323
Size: 703 389 2708 729 473 260 450 639
Cuts at: 8323 8347 9016 9538 9775 10142 10527 10544 11996
Size: 24 669 522 237 367 385 17 1452
Cuts at: 11996 12082 12094 12440 1972
Size: 86 12 346 1977
Fragments arranged by size:
2708 1977 1452 729 703 669 639 522
473 450 389 385 367 346 260 237
86 24 17 12
Bsu36I CC'TnA_GG
Cuts at: 7001 9712 7001
Size: 2711 9734
BtgI C'CryG_G
Cuts at: 513 1065 2004 2022 8130 513
Size: 552 939 18 6108 4828
Fragments arranged by size:
6108 4828 939 552 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 6581 487
Size: 683 56 5355 6351
Fragments arranged by size:
6351 5355 683 56
BtsI GCAGTG_nn'
Cuts at: 2725 8807 9961 10755 10775 2725
Size: 6082 1154 794 20 4395
Fragments arranged by size:
6082 4395 1154 794 20
ClaI AT'CG_AT
Cuts at: 1554 2217 1554
Size: 663 11782
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 12410
DraI TTT'AAA
Cuts at: 5418 7542 8671 9749 5418
Size: 2124 1129 1078 8114
Fragments arranged by size:
8114 2124 1129 1078
DraIII CAC_nnn'GTG
Cuts at: 3515 3515
Size: 12445
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2111 11452 809
Size: 507 795 9341 1802
Fragments arranged by size:
9341 1802 795 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2025 2045 9892
Size: 54 1425 50 275 18 195 20 7847
Cuts at: 9892 10781 12410 8
Size: 889 1629 43
Fragments arranged by size:
7847 1629 1425 889 275 195 54 50
43 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2045 9892 1537
Size: 275 233 7847 4090
Fragments arranged by size:
7847 4090 275 233
EarI CTCTTCn'nnn_
Cuts at: 1353 3220 3256 4227 5367 5763 5949 6351 7609
Size: 1867 36 971 1140 396 186 402 1258
Cuts at: 7609 7949 8454 10375 1353
Size: 340 505 1921 3423
Fragments arranged by size:
3423 1921 1867 1258 1140 971 505 402
396 340 186 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 11032 11406 11552 387
Size: 1064 138 435 9008 374 146 1280
Fragments arranged by size:
9008 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 4237 4237
Size: 12445
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2256 2864 2916 2948 3146 3896 3902
Size: 393 624 608 52 32 198 750 6
Cuts at: 3902 4007 4088 4212 4251 4296 4538 4625 5873
Size: 105 81 124 39 45 242 87 1248
Cuts at: 5873 6375 6719 7139 7658 8180 8951 10508 11105
Size: 502 344 420 519 522 771 1557 597
Cuts at: 11105 11892 12160 1239
Size: 787 268 1524
Fragments arranged by size:
1557 1524 1248 787 771 750 624 608
597 522 519 502 420 393 344 268
242 198 124 105 87 81 52 45
39 32 6
EcoO109I rG'GnC_Cy
Cuts at: 5280 6730 8632 9695 5280
Size: 1450 1902 1063 8030
Fragments arranged by size:
8030 1902 1450 1063
EcoRI G'AATT_C
Cuts at: 2874 2884 4382 8027 8135 9451 9754 10132 2874
Size: 10 1498 3645 108 1316 303 378 5187
Fragments arranged by size:
5187 3645 1498 1316 378 303 108 10
EcoRV GAT'ATC
Cuts at: 9186 9186
Size: 12445
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2087 3176 3285 4328 5288 6026 6305 6380 8713
Size: 1089 109 1043 960 738 279 75 2333
Cuts at: 8713 2087
Size: 5819
Fragments arranged by size:
5819 2333 1089 1043 960 738 279 109
75
FspI TGC'GCA
Cuts at: 10951 10951
Size: 12445
HaeII r_GCGC'y
Cuts at: 1678 2745 11592 1678
Size: 1067 8847 2531
Fragments arranged by size:
8847 2531 1067
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2460 2492 2793 2825 3091
Size: 32 135 32 888 32 301 32 266
Cuts at: 3091 3123 4084 4116 4444 4476 5948 5950 5980
Size: 32 961 32 328 32 1472 2 30
Cuts at: 5980 5982 7325 7357 7960 7992 8291 8323 8624
Size: 2 1343 32 603 32 299 32 301
Cuts at: 8624 8656 9130 9162 9569 9601 11086 11118 11160
Size: 32 474 32 407 32 1485 32 42
Cuts at: 11160 11192 1373
Size: 32 2626
Fragments arranged by size:
2626 1485 1472 1343 961 888 603 474
407 328 301 301 299 266 135 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 30
2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 2868 4798 6651 6939 8775 9161
Size: 988 281 930 1930 1853 288 1836 386
Cuts at: 9161 9789 10035 669
Size: 628 246 3079
Fragments arranged by size:
3079 1930 1853 1836 988 930 628 386
288 281 246
HindIII A'AGCT_T
Cuts at: 748 2785 3359 4140 4747 7507 8492 9029 748
Size: 2037 574 781 607 2760 985 537 4164
Fragments arranged by size:
4164 2760 2037 985 781 607 574 537
HpaI GTT'AAC
Cuts at: 2868 4798 8775 10035 2868
Size: 1930 3977 1260 5278
Fragments arranged by size:
5278 3977 1930 1260
KpnI G_GTAC'C
Cuts at: 9770 9770
Size: 12445
MfeI C'AATT_G
Cuts at: 3128 5132 10044 3128
Size: 2004 4912 5529
Fragments arranged by size:
5529 4912 2004
MluI A'CGCG_T
Cuts at: 12321 12321
Size: 12445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3936 5119 11559 11743 1305
Size: 2631 1183 6440 184 2007
Fragments arranged by size:
6440 2631 2007 1183 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 10623
Fragments arranged by size:
10623 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2169 7212 10403 10762 10921 518
Size: 648 1003 5043 3191 359 159 2042
Fragments arranged by size:
5043 3191 2042 1003 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 4603 6031 6833 9243 9861 10538 11686
Size: 444 2553 1428 802 2410 618 677 1148
Cuts at: 11686 11931 1606
Size: 245 2120
Fragments arranged by size:
2553 2410 2120 1428 1148 802 677 618
444 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 8130 513
Size: 552 939 6126 4828
Fragments arranged by size:
6126 4828 939 552
NdeI CA'TA_TG
Cuts at: 387 4407 4917 387
Size: 4020 510 7915
Fragments arranged by size:
7915 4020 510
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12445
NotI GC'GGCC_GC
Cuts at: 2045 9892 2045
Size: 7847 4598
NsiI A_TGCA'T
Cuts at: 7116 7275 8838 7116
Size: 159 1563 10723
Fragments arranged by size:
10723 1563 159
NspI r_CATG'y
Cuts at: 1132 1229 7114 7273 9803 11348 1132
Size: 97 5885 159 2530 1545 2229
Fragments arranged by size:
5885 2530 2229 1545 159 97
PasI CC'CwG_GG
Cuts at: 1348 2325 1348
Size: 977 11468
PciI A'CATG_T
Cuts at: 11344 11344
Size: 12445
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3142 8344 8622 1108
Size: 2034 5202 278 4931
Fragments arranged by size:
5202 4931 2034 278
PfoI T'CCnGG_A
Cuts at: 4904 6066 4904
Size: 1162 11283
PmeI GTTT'AAAC
Cuts at: 9749 9749
Size: 12445
PmlI CAC'GTG
Cuts at: 4568 4568
Size: 12445
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10514 10546 12027 12059 10514
Size: 32 1481 32 10900
Fragments arranged by size:
10900 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 5280 8632 9695 5280
Size: 3352 1063 8030
Fragments arranged by size:
8030 3352 1063
PshAI GACnn'nnGTC
Cuts at: 1613 6833 1613
Size: 5220 7225
PsiI TTA'TAA
Cuts at: 4365 8855 9128 10015 4365
Size: 4490 273 887 6795
Fragments arranged by size:
6795 4490 887 273
PspXI vC'TCGA_Gb
Cuts at: 6644 6644
Size: 12445
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5784 5816 9516 9548 5784
Size: 32 3700 32 8681
Fragments arranged by size:
8681 3700 32 32
PstI C_TGCA'G
Cuts at: 830 2749 4683 5023 9797 830
Size: 1919 1934 340 4774 3478
Fragments arranged by size:
4774 3478 1934 1919 340
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12445
PvuII CAG'CTG
Cuts at: 4603 6031 6833 4603
Size: 1428 802 10215
Fragments arranged by size:
10215 1428 802
SacI G_AGCT'C
Cuts at: 4239 4239
Size: 12445
SalI G'TCGA_C
Cuts at: 1936 9787 1936
Size: 7851 4594
SapI GCTCTTCn'nnn_
Cuts at: 4227 4227
Size: 12445
SbfI CC_TGCA'GG
Cuts at: 9797 9797
Size: 12445
ScaI AGT'ACT
Cuts at: 2977 8424 8964 10693 12405 2977
Size: 5447 540 1729 1712 3017
Fragments arranged by size:
5447 3017 1729 1712 540
SfcI C'TryA_G
Cuts at: 826 1045 2745 3710 3825 4644 4679 5019 5613
Size: 219 1700 965 115 819 35 340 594
Cuts at: 5613 6246 6269 9105 9793 10928 11609 11800 826
Size: 633 23 2836 688 1135 681 191 1471
Fragments arranged by size:
2836 1700 1471 1135 965 819 688 681
633 594 340 219 191 115 35 23
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12445
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 12445
SmaI CCC'GGG
Cuts at: 1872 9772 1872
Size: 7900 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2759 4439 4713 4815 4838 5474
Size: 197 926 816 1680 274 102 23 636
Cuts at: 5474 6644 6798 6821 7043 7403 7652 8873 9631
Size: 1170 154 23 222 360 249 1221 758
Cuts at: 9631 10549 11450 11712 820
Size: 918 901 262 1553
Fragments arranged by size:
1680 1553 1221 1170 926 918 901 816
758 636 360 274 262 249 222 197
154 102 23 23
SnaBI TAC'GTA
Cuts at: 493 10236 493
Size: 9743 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12445
SphI G_CATG'C
Cuts at: 1132 9803 1132
Size: 8671 3774
SspI AAT'ATT
Cuts at: 5 52 5158 10369 5
Size: 47 5106 5211 2081
Fragments arranged by size:
5211 5106 2081 47
StuI AGG'CCT
Cuts at: 1517 5619 9642 1517
Size: 4102 4023 4320
Fragments arranged by size:
4320 4102 4023
StyI C'CwwG_G
Cuts at: 513 1065 2004 2889 3277 5880 5973 6606 8130
Size: 552 939 885 388 2603 93 633 1524
Cuts at: 8130 8697 9698 9884 513
Size: 567 1001 186 3074
Fragments arranged by size:
3074 2603 1524 1001 939 885 633 567
552 388 186 93
SwaI ATTT'AAAT
Cuts at: 7542 7542
Size: 12445
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4502 4541 7863 10472 10489 10642 10827
Size: 1255 2922 39 3322 2609 17 153 185
Cuts at: 10827 11272 325
Size: 445 1498
Fragments arranged by size:
3322 2922 2609 1498 1255 445 185 153
39 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2975 3169 5365
Size: 274 80 33 51 1443 998 194 2196
Cuts at: 5365 6721 8422 8962 9250 10691 12403 96
Size: 1356 1701 540 288 1441 1712 138
Fragments arranged by size:
2196 1712 1701 1443 1441 1356 998 540
288 274 194 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2862 2894 2914 2946 6842 6874 12405 12437 2862
Size: 32 20 32 3896 32 5531 32 2870
Fragments arranged by size:
5531 3896 2870 32 32 32 32 20
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 12445
XbaI T'CTAG_A
Cuts at: 3622 7934 9781 3622
Size: 4312 1847 6286
Fragments arranged by size:
6286 4312 1847
XhoI C'TCGA_G
Cuts at: 1943 6644 1943
Size: 4701 7744
XmaI C'CCGG_G
Cuts at: 1870 9770 1870
Size: 7900 4545
XmnI GAAnn'nnTTC
Cuts at: 4281 5937 6288 6584 7318 7392 8543 9484 10574
Size: 1656 351 296 734 74 1151 941 1090
Cuts at: 10574 4281
Size: 6152
Fragments arranged by size:
6152 1656 1151 1090 941 734 351 296
74
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 10253 276
Size: 53 83 186 936 8719 2468
Fragments arranged by size:
8719 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BsiWI BstZ17I
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI RsrII SacII SanDI SexAI SfiI SfoI
SrfI XcmI