Restriction map for
pFN21AB0065
(Circular) (Six-base) MAPSORT of: pFN21AB0065.seq Check: 6828 from: 1 to: 10828
pFN21AB0065 10828 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4819 7735 4819
Size: 2916 7912
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3432 7832 8008 8638
Size: 53 83 186 936 1896 4400 176 630
Cuts at: 8638 278
Size: 2468
Fragments arranged by size:
4400 2468 1896 936 630 186 176 83
53
AccI GT'mk_AC
Cuts at: 1937 5186 8171 1937
Size: 3249 2985 4594
Fragments arranged by size:
4594 3249 2985
Acc65I G'GTAC_C
Cuts at: 8149 8149
Size: 10828
AclI AA'CG_TT
Cuts at: 1563 8955 9328 1563
Size: 7392 373 3063
Fragments arranged by size:
7392 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2122 2132 2141 2187 3195 3296 3561 3769
Size: 490 10 9 46 1008 101 265 208
Cuts at: 3769 4337 4450 5779 5895 6671 6702 7897 8016
Size: 568 113 1329 116 776 31 1195 119
Cuts at: 8016 8891 10275 10543 1632
Size: 875 1384 268 1917
Fragments arranged by size:
1917 1384 1329 1195 1008 875 776 568
490 268 265 208 119 116 113 101
46 31 10 9
AfeI AGC'GCT
Cuts at: 2051 2051
Size: 10828
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10631
AflIII A'CryG_T
Cuts at: 1203 5569 9727 10704 1203
Size: 4366 4158 977 1327
Fragments arranged by size:
4366 4158 1327 977
AgeI A'CCGG_T
Cuts at: 9659 9659
Size: 10828
AhdI GACnn_n'nnGTC
Cuts at: 6575 9557 6575
Size: 2982 7846
AleI CACnn'nnGTG
Cuts at: 1166 4431 4853 1166
Size: 3265 422 7141
Fragments arranged by size:
7141 3265 422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 7767 7812 1184
Size: 6583 45 4200
Fragments arranged by size:
6583 4200 45
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2487 2519 2487
Size: 32 10796
AlwNI CAG_nnn'CTG
Cuts at: 2149 2176 2327 4337 6592 6725 6766 7994 8571
Size: 27 151 2010 2255 133 41 1228 577
Cuts at: 8571 10143 2149
Size: 1572 2834
Fragments arranged by size:
2834 2255 2010 1572 1228 577 151 133
41 27
ApaI G_GGCC'C
Cuts at: 2035 7523 2035
Size: 5488 5340
ApaLI G'TGCA_C
Cuts at: 8885 10041 8885
Size: 1156 9672
ApoI r'AATT_y
Cuts at: 1493 2366 2565 4666 8137 8363 8515 10624 1493
Size: 873 199 2101 3471 226 152 2109 1697
Fragments arranged by size:
3471 2109 2101 1697 873 226 199 152
AseI AT'TA_AT
Cuts at: 160 1032 2343 5031 5175 7322 9382 160
Size: 872 1311 2688 144 2147 2060 1606
Fragments arranged by size:
2688 2147 2060 1606 1311 872 144
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10828
AvaI C'yCGr_G
Cuts at: 1870 1943 8153 1870
Size: 73 6210 4545
Fragments arranged by size:
6210 4545 73
AvrII C'CTAG_G
Cuts at: 5056 7210 5056
Size: 2154 8674
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7233 7266 7233
Size: 33 10795
BamHI G'GATC_C
Cuts at: 4018 4159 7427 8158 8525 4018
Size: 141 3268 731 367 6321
Fragments arranged by size:
6321 3268 731 367 141
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3011 4204 5123 5845 6959
Size: 325 217 627 1224 1193 919 722 1114
Cuts at: 6959 8149 9604 618
Size: 1190 1455 1842
Fragments arranged by size:
1842 1455 1224 1193 1190 1114 919 722
627 325 217
BanII G_rGCy'C
Cuts at: 1391 2035 2132 2424 3414 4940 4964 7523 8147
Size: 644 97 292 990 1526 24 2559 624
Cuts at: 8147 1391
Size: 4072
Fragments arranged by size:
4072 2559 1526 990 644 624 292 97
24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2100 3432 5236 6121 6659 7018 7303
Size: 971 201 1332 1804 885 538 359 285
Cuts at: 7303 7344 8004 928
Size: 41 660 3752
Fragments arranged by size:
3752 1804 1332 971 885 660 538 359
285 201 41
BbvCI CC'TCA_GC
Cuts at: 2682 2682
Size: 10828
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 9019 9053 1903
Size: 34 7082 34 3678
Fragments arranged by size:
7082 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3564 8720 9930 3564
Size: 5156 1210 4462
Fragments arranged by size:
5156 4462 1210
BclI T'GATC_A
Cuts at: 1548 2371 2560 2593 2875 5935 1548
Size: 823 189 33 282 3060 6441
Fragments arranged by size:
6441 3060 823 282 189 33
BfrBI ATG'CAT
Cuts at: 7472 7724 7472
Size: 252 10576
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5843 9439 136
Size: 107 122 71 631 605 4171 3596 1525
Fragments arranged by size:
4171 3596 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2929 3982 7032 10823 2929
Size: 1053 3050 3791 2934
Fragments arranged by size:
3791 3050 2934 1053
BlpI GC'TnA_GC
Cuts at: 4356 5531 8245 4356
Size: 1175 2714 6939
Fragments arranged by size:
6939 2714 1175
Bme1580I G_kGCm'C
Cuts at: 1790 2035 4493 5898 6268 7523 8889 10045 1790
Size: 245 2458 1405 370 1255 1366 1156 2573
Fragments arranged by size:
2573 2458 1405 1366 1255 1156 370 245
BmgBI CAC'GTC
Cuts at: 1318 7765 1318
Size: 6447 4381
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5844 6754 7544
Size: 416 53 83 399 24 4427 910 790
Cuts at: 7544 7830 8559 9517 442
Size: 286 729 958 1753
Fragments arranged by size:
4427 1753 958 910 790 729 416 399
286 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2460 2883 4133 4724 5488 5637 6083 6476
Size: 1221 423 1250 591 764 149 446 393
Cuts at: 6476 7044 7266 7572 7632 7776 9488 1239
Size: 568 222 306 60 144 1712 2579
Fragments arranged by size:
2579 1712 1250 1221 764 591 568 446
423 393 306 222 149 144 60
Bpu10I CC'TnA_GC
Cuts at: 2682 4398 6926 7906 2682
Size: 1716 2528 980 5604
Fragments arranged by size:
5604 2528 1716 980
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4316 4554 7078 8953 9818 10116 4316
Size: 238 2524 1875 865 298 5028
Fragments arranged by size:
5028 2524 1875 865 298 238
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2895 3273 7305 9491 882
Size: 617 1396 378 4032 2186 2219
Fragments arranged by size:
4032 2219 2186 1396 617 378
BsaAI yAC'GTr
Cuts at: 493 1204 3979 8619 493
Size: 711 2775 4640 2702
Fragments arranged by size:
4640 2775 2702 711
BsaBI GATnn'nnATC
Cuts at: 10822 10822
Size: 10828
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3429 7103 7829 8005
Size: 53 83 186 936 1896 3674 726 176
Cuts at: 8005 8635 9017 9652 275
Size: 630 382 635 1451
Fragments arranged by size:
3674 1896 1451 936 726 635 630 382
186 176 83 53
BsaWI w'CCGG_w
Cuts at: 2749 6796 9261 9659 9933 10080 10491 2749
Size: 4047 2465 398 274 147 411 3086
Fragments arranged by size:
4047 3086 2465 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 7148 7178 7239 7269 7749
Size: 30 165 30 5745 30 61 30 480
Cuts at: 7749 7779 1178
Size: 30 4227
Fragments arranged by size:
5745 4227 480 165 61 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2414 2499 2880 3049 3400 4635 7169 7569
Size: 1228 85 381 169 351 1235 2534 400
Cuts at: 7569 7773 7854 1186
Size: 204 81 4160
Fragments arranged by size:
4160 2534 1235 1228 400 381 351 204
169 85 81
BseYI C'CCAG_C
Cuts at: 2248 2491 6098 6919 6955 10031 10527 2248
Size: 243 3607 821 36 3076 496 2549
Fragments arranged by size:
3607 3076 2549 821 496 243 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4917 5753 5908 6750 1163
Size: 573 3181 836 155 842 5241
Fragments arranged by size:
5241 3181 842 836 573 155
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8278 9039 10067 1540
Size: 275 186 6277 761 1028 2301
Fragments arranged by size:
6277 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2424 3372 3414 4327 4964 8889 8974 10045 2424
Size: 948 42 913 637 3925 85 1071 3207
Fragments arranged by size:
3925 3207 1071 948 913 637 85 42
BsmI GAATG_Cn'
Cuts at: 4095 6247 7474 8339 8432 4095
Size: 2152 1227 865 93 6491
Fragments arranged by size:
6491 2152 1227 865 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3786 7755 3786
Size: 3969 6859
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2132 2424 3372 3414 4327 4493
Size: 399 245 97 292 948 42 913 166
Cuts at: 4493 4940 4964 5898 6268 7523 8147 8889 8974
Size: 447 24 934 370 1255 624 742 85
Cuts at: 8974 10045 1391
Size: 1071 2174
Fragments arranged by size:
2174 1255 1071 948 934 913 742 624
447 399 370 292 245 166 97 85
42 24
BspEI T'CCGG_A
Cuts at: 10491 10491
Size: 10828
BspHI T'CATG_A
Cuts at: 8717 10447 8717
Size: 1730 9098
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2362 4819 4956 6869 7735 8183 844
Size: 1518 2457 137 1913 866 448 3489
Fragments arranged by size:
3489 2457 1913 1518 866 448 137
BsrBI CCG'CTC
Cuts at: 1394 1823 8715 1394
Size: 429 6892 3507
Fragments arranged by size:
6892 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2675 2981 3174 4215 5542 7602 8028
Size: 1183 1427 306 193 1041 1327 2060 426
Cuts at: 8028 9323 9497 65
Size: 1295 174 1396
Fragments arranged by size:
2060 1427 1396 1327 1295 1183 1041 426
306 193 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 9472 9659 1700
Size: 110 7662 187 2869
Fragments arranged by size:
7662 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2167 96
Size: 2071 8757
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10828
BssSI C'ACGA_G
Cuts at: 5214 8888 9900 5214
Size: 3674 1012 6142
Fragments arranged by size:
6142 3674 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3011 3038 6728 6842 794
Size: 2217 27 3690 114 4780
Fragments arranged by size:
4780 3690 2217 114 27
BstBI TT'CG_AA
Cuts at: 5104 9632 5104
Size: 4528 6300
BstEII G'GTnAC_C
Cuts at: 1184 4247 6620 1184
Size: 3063 2373 5392
Fragments arranged by size:
5392 3063 2373
BstXI CCAn_nnnn'nTGG
Cuts at: 2540 3043 5458 2540
Size: 503 2415 7910
Fragments arranged by size:
7910 2415 503
BstYI r'GATC_y
Cuts at: 1972 2795 2929 3982 4018 4159 7032 7427 8158
Size: 823 134 1053 36 141 2873 395 731
Cuts at: 8158 8525 8910 8927 10379 10465 10477 10823 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2873 1977 1452 1053 823 731 395 385
367 346 141 134 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2969 4650 5488 2969
Size: 1681 838 8309
Fragments arranged by size:
8309 1681 838
BtgI C'CryG_G
Cuts at: 513 1065 6558 513
Size: 552 5493 4783
Fragments arranged by size:
5493 4783 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4046 7531 487
Size: 683 56 2820 3485 3784
Fragments arranged by size:
3784 3485 2820 683 56
BtsI GCAGTG_nn'
Cuts at: 2530 2902 2953 3036 3250 5896 6315 8344 9138
Size: 372 51 83 214 2646 419 2029 794
Cuts at: 9138 9158 2530
Size: 20 4200
Fragments arranged by size:
4200 2646 2029 794 419 372 214 83
51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10828
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10793
DraI TTT'AAA
Cuts at: 6439 8132 6439
Size: 1693 9135
DraIII CAC_nnn'GTG
Cuts at: 6515 7574 6515
Size: 1059 9769
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 9835 809
Size: 507 8519 1802
Fragments arranged by size:
8519 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4077 5140 6321
Size: 54 1425 50 275 18 2247 1063 1181
Cuts at: 6321 8275 9164 10793 8
Size: 1954 889 1629 43
Fragments arranged by size:
2247 1954 1629 1425 1181 1063 889 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8275 1537
Size: 275 6463 4090
Fragments arranged by size:
6463 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2141 3788 4053 4469 4593 5175 5283 5317
Size: 788 1647 265 416 124 582 108 34
Cuts at: 5317 5385 8758 1353
Size: 68 3373 3423
Fragments arranged by size:
3423 3373 1647 788 582 416 265 124
108 68 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9415 9789 9935 387
Size: 1064 138 7826 374 146 1280
Fragments arranged by size:
7826 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2422 3412 4962 2422
Size: 990 1550 8288
Fragments arranged by size:
8288 1550 990
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2122 2132 2141 2187 2460 2883 3195
Size: 393 490 10 9 46 273 423 312
Cuts at: 3195 3296 3561 3769 4133 4337 4450 4724 5488
Size: 101 265 208 364 204 113 274 764
Cuts at: 5488 5637 5779 5895 6083 6476 6671 6702 7044
Size: 149 142 116 188 393 195 31 342
Cuts at: 7044 7266 7572 7632 7776 7897 8016 8891 9488
Size: 222 306 60 144 121 119 875 597
Cuts at: 9488 10275 10543 1239
Size: 787 268 1524
Fragments arranged by size:
1524 875 787 764 597 490 423 393
393 364 342 312 306 274 273 268
265 222 208 204 195 188 149 144
142 121 119 116 113 101 60 46
31 10 9
EcoO109I rG'GnC_Cy
Cuts at: 2032 2324 3758 4775 7141 7519 7520 7859 2032
Size: 292 1434 1017 2366 378 1 339 5001
Fragments arranged by size:
5001 2366 1434 1017 378 339 292 1
EcoRI G'AATT_C
Cuts at: 8137 8515 8137
Size: 378 10450
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5809 5809
Size: 10828
FspI TGC'GCA
Cuts at: 9334 9334
Size: 10828
HaeII r_GCGC'y
Cuts at: 1678 2053 9975 1678
Size: 375 7922 2531
Fragments arranged by size:
7922 2531 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2248 2280 2799 2831 3401
Size: 32 135 32 676 32 519 32 570
Cuts at: 3401 3433 3491 3523 4003 4035 4852 4884 5223
Size: 32 58 32 480 32 817 32 339
Cuts at: 5223 5255 5509 5541 5610 5642 6196 6228 6561
Size: 32 254 32 69 32 554 32 333
Cuts at: 6561 6593 7017 7049 9469 9501 9543 9575 1373
Size: 32 424 32 2420 32 42 32 2626
Fragments arranged by size:
2626 2420 817 676 570 554 519 480
424 339 333 254 135 69 58 42
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4276 7708 8172 8418 669
Size: 988 281 2338 3432 464 246 3079
Fragments arranged by size:
3432 3079 2338 988 464 281 246
HindIII A'AGCT_T
Cuts at: 748 5302 748
Size: 4554 6274
HpaI GTT'AAC
Cuts at: 4276 7708 8418 4276
Size: 3432 710 6686
Fragments arranged by size:
6686 3432 710
KpnI G_GTAC'C
Cuts at: 8153 8153
Size: 10828
MfeI C'AATT_G
Cuts at: 4164 5437 8427 4164
Size: 1273 2990 6565
Fragments arranged by size:
6565 2990 1273
MluI A'CGCG_T
Cuts at: 10704 10704
Size: 10828
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 3745 6559 7903 9942 10126 1305
Size: 684 1756 2814 1344 2039 184 2007
Fragments arranged by size:
2814 2039 2007 1756 1344 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4079 10
Size: 54 1425 343 2247 6759
Fragments arranged by size:
6759 2247 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2343 2538 3843 4431 4769 4853 5121
Size: 648 1177 195 1305 588 338 84 268
Cuts at: 5121 6240 7739 8114 8786 9145 9304 518
Size: 1119 1499 375 672 359 159 2042
Fragments arranged by size:
2042 1499 1305 1177 1119 672 648 588
375 359 338 268 195 159 84
MspA1I CmG'CkG
Cuts at: 1606 2176 2252 3569 3774 4928 5591 6003 6419
Size: 570 76 1317 205 1154 663 412 416
Cuts at: 6419 6725 8244 8921 10069 10314 1606
Size: 306 1519 677 1148 245 2120
Fragments arranged by size:
2120 1519 1317 1154 1148 677 663 570
416 412 306 245 205 76
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 10276
NdeI CA'TA_TG
Cuts at: 387 4695 6051 387
Size: 4308 1356 5164
Fragments arranged by size:
5164 4308 1356
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10828
NotI GC'GGCC_GC
Cuts at: 8275 8275
Size: 10828
NsiI A_TGCA'T
Cuts at: 7474 7726 7474
Size: 252 10576
NspI r_CATG'y
Cuts at: 1132 1229 2042 5573 7002 8186 9731 1132
Size: 97 813 3531 1429 1184 1545 2229
Fragments arranged by size:
3531 2229 1545 1429 1184 813 97
PasI CC'CwG_GG
Cuts at: 1348 2395 1348
Size: 1047 9781
PciI A'CATG_T
Cuts at: 5569 9727 5569
Size: 4158 6670
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2300 3847 6782 7424 1108
Size: 1192 1547 2935 642 4512
Fragments arranged by size:
4512 2935 1547 1192 642
PfoI T'CCnGG_A
Cuts at: 6116 6331 6116
Size: 215 10613
PmeI GTTT'AAAC
Cuts at: 8132 8132
Size: 10828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8897 8929 10410 10442 8897
Size: 32 1481 32 9283
Fragments arranged by size:
9283 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2324 3758 7859 2324
Size: 1434 4101 5293
Fragments arranged by size:
5293 4101 1434
PshAI GACnn'nnGTC
Cuts at: 1613 8004 1613
Size: 6391 4437
PsiI TTA'TAA
Cuts at: 4190 5732 8398 4190
Size: 1542 2666 6620
Fragments arranged by size:
6620 2666 1542
PspOMI G'GGCC_C
Cuts at: 2031 7519 2031
Size: 5488 5340
PstI C_TGCA'G
Cuts at: 830 2127 2634 2722 5278 6163 7191 8180 830
Size: 1297 507 88 2556 885 1028 989 3478
Fragments arranged by size:
3478 2556 1297 1028 989 885 507 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10828
PvuII CAG'CTG
Cuts at: 2176 2252 3569 4928 5591 6003 6419 6725 2176
Size: 76 1317 1359 663 412 416 306 6279
Fragments arranged by size:
6279 1359 1317 663 416 412 306 76
SacI G_AGCT'C
Cuts at: 2424 3414 4964 2424
Size: 990 1550 8288
Fragments arranged by size:
8288 1550 990
SalI G'TCGA_C
Cuts at: 1936 8170 1936
Size: 6234 4594
SapI GCTCTTCn'nnn_
Cuts at: 3788 3788
Size: 10828
SbfI CC_TGCA'GG
Cuts at: 8180 8180
Size: 10828
ScaI AGT'ACT
Cuts at: 4683 9076 10788 4683
Size: 4393 1712 4723
Fragments arranged by size:
4723 4393 1712
SexAI A'CCwGG_T
Cuts at: 2184 2326 2184
Size: 142 10686
SfcI C'TryA_G
Cuts at: 826 1045 2123 2630 2718 3873 4305 5274 5706
Size: 219 1078 507 88 1155 432 969 432
Cuts at: 5706 6159 6597 7129 7187 8176 9311 9992 10183
Size: 453 438 532 58 989 1135 681 191
Cuts at: 10183 826
Size: 1471
Fragments arranged by size:
1471 1155 1135 1078 989 969 681 532
507 453 438 432 432 219 191 88
58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10828
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10828
SmaI CCC'GGG
Cuts at: 1872 8155 1872
Size: 6283 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4295 4569 7057 8932 9833 10095
Size: 197 926 2352 274 2488 1875 901 262
Cuts at: 10095 820
Size: 1553
Fragments arranged by size:
2488 2352 1875 1553 926 901 274 262
197
SnaBI TAC'GTA
Cuts at: 493 8619 493
Size: 8126 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10828
SphI G_CATG'C
Cuts at: 1132 2042 8186 1132
Size: 910 6144 3774
Fragments arranged by size:
6144 3774 910
SspI AAT'ATT
Cuts at: 5 52 2194 2218 2499 8752 5
Size: 47 2142 24 281 6253 2081
Fragments arranged by size:
6253 2142 2081 281 47 24
StuI AGG'CCT
Cuts at: 1517 2445 7209 1517
Size: 928 4764 5136
Fragments arranged by size:
5136 4764 928
StyI C'CwwG_G
Cuts at: 513 1065 2446 3157 5056 5741 6465 7210 8267
Size: 552 1381 711 1899 685 724 745 1057
Cuts at: 8267 513
Size: 3074
Fragments arranged by size:
3074 1899 1381 1057 745 724 711 685
552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3049 3928 3935 4266 5863 6126 6947
Size: 1255 1469 879 7 331 1597 263 821
Cuts at: 6947 7370 7620 7712 8078 8855 8872 9025 9210
Size: 423 250 92 366 777 17 153 185
Cuts at: 9210 9655 325
Size: 445 1498
Fragments arranged by size:
1597 1498 1469 1255 879 821 777 445
423 366 331 263 250 185 153 92
17 7
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2167 4052 4681
Size: 274 80 33 51 1443 190 1885 629
Cuts at: 4681 5787 7433 9074 10786 96
Size: 1106 1646 1641 1712 138
Fragments arranged by size:
1885 1712 1646 1641 1443 1106 629 274
190 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3351 3383 7148 7180 7239 7271 7749 7781 10788
Size: 32 3765 32 59 32 478 32 3007
Cuts at: 10788 10820 3351
Size: 32 3359
Fragments arranged by size:
3765 3359 3007 478 59 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7265 1316
Size: 5949 4879
XbaI T'CTAG_A
Cuts at: 6199 8050 8164 6199
Size: 1851 114 8863
Fragments arranged by size:
8863 1851 114
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10828
XmaI C'CCGG_G
Cuts at: 1870 8153 1870
Size: 6283 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 4062 5233 5685 6372 7341 8957 3782
Size: 280 1171 452 687 969 1616 5653
Fragments arranged by size:
5653 1616 1171 969 687 452 280
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3430 7830 8006 8636
Size: 53 83 186 936 1896 4400 176 630
Cuts at: 8636 276
Size: 2468
Fragments arranged by size:
4400 2468 1896 936 630 186 176 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AscI BbeI BplI BsiWI BstZ17I EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI
SwaI XcmI