Restriction map for
pFN21AB0063
(Circular) (Six-base) MAPSORT of: pFN21AB0063.seq Check: 678 from: 1 to: 11308
pFN21AB0063 11308 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2021 2999 3047 8324 2021
Size: 978 48 5277 5005
Fragments arranged by size:
5277 5005 978 48
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4031 9118 278
Size: 53 83 186 936 2495 5087 2468
Fragments arranged by size:
5087 2495 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3168 6522 8651 1937
Size: 1231 3354 2129 4594
Fragments arranged by size:
4594 3354 2129 1231
Acc65I G'GTAC_C
Cuts at: 8629 8629
Size: 11308
AclI AA'CG_TT
Cuts at: 1563 9435 9808 1563
Size: 7872 373 3063
Fragments arranged by size:
7872 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2229 2488 2532 4170 4409 4613 4961 5713
Size: 597 259 44 1638 239 204 348 752
Cuts at: 5713 8327 8560 9371 10755 11023 1632
Size: 2614 233 811 1384 268 1917
Fragments arranged by size:
2614 1917 1638 1384 811 752 597 348
268 259 239 233 204 44
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 11111
AflIII A'CryG_T
Cuts at: 1203 2789 4641 4705 5200 10207 11184 1203
Size: 1586 1852 64 495 5007 977 1327
Fragments arranged by size:
5007 1852 1586 1327 977 495 64
AgeI A'CCGG_T
Cuts at: 7748 10139 7748
Size: 2391 8917
AhdI GACnn_n'nnGTC
Cuts at: 3551 3815 4027 10037 3551
Size: 264 212 6010 4822
Fragments arranged by size:
6010 4822 264 212
AleI CACnn'nnGTG
Cuts at: 1166 3153 3987 1166
Size: 1987 834 8487
Fragments arranged by size:
8487 1987 834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2442 1184
Size: 1258 10050
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3989 4021 4562 4594 8523 8555 3989
Size: 32 541 32 3929 32 6742
Fragments arranged by size:
6742 3929 541 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2354 2676 3516 3702 3795 8448 9051 10623 2354
Size: 322 840 186 93 4653 603 1572 3039
Fragments arranged by size:
4653 3039 1572 840 603 322 186 93
ApaI G_GGCC'C
Cuts at: 4382 7623 7887 4382
Size: 3241 264 7803
Fragments arranged by size:
7803 3241 264
ApaLI G'TGCA_C
Cuts at: 9365 10521 9365
Size: 1156 10152
ApoI r'AATT_y
Cuts at: 1493 4160 5036 6725 7174 8162 8617 8843 8995
Size: 2667 876 1689 449 988 455 226 152
Cuts at: 8995 11104 1493
Size: 2109 1697
Fragments arranged by size:
2667 2109 1697 1689 988 876 455 449
226 152
AseI AT'TA_AT
Cuts at: 160 1032 6624 9862 160
Size: 872 5592 3238 1606
Fragments arranged by size:
5592 3238 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11308
AvaI C'yCGr_G
Cuts at: 1870 1943 3820 4374 6217 6224 6356 7574 7734
Size: 73 1877 554 1843 7 132 1218 160
Cuts at: 7734 8633 1870
Size: 899 4545
Fragments arranged by size:
4545 1877 1843 1218 899 554 160 132
73 7
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7919 7952 7919
Size: 33 11275
BamHI G'GATC_C
Cuts at: 2186 4201 8638 9005 2186
Size: 2015 4437 367 4489
Fragments arranged by size:
4489 4437 2015 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2236 2987 7198 8629 10084
Size: 325 217 627 449 751 4211 1431 1455
Cuts at: 10084 618
Size: 1842
Fragments arranged by size:
4211 1842 1455 1431 751 627 449 325
217
BanII G_rGCy'C
Cuts at: 1391 2253 2568 2943 3222 4382 5679 7139 7623
Size: 862 315 375 279 1160 1297 1460 484
Cuts at: 7623 7634 7844 7887 8220 8627 1391
Size: 11 210 43 333 407 4072
Fragments arranged by size:
4072 1460 1297 1160 862 484 407 375
333 315 279 210 43 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2293 2397 2934 3027 3492 3864 4920
Size: 971 394 104 537 93 465 372 1056
Cuts at: 4920 5027 6934 8032 928
Size: 107 1907 1098 4204
Fragments arranged by size:
4204 1907 1098 1056 971 537 465 394
372 107 104 93
BbvCI CC'TCA_GC
Cuts at: 4812 5478 6297 8025 4812
Size: 666 819 1728 8095
Fragments arranged by size:
8095 1728 819 666
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2583 2617 2701 2735 2807 2841 9499
Size: 34 646 34 84 34 72 34 6658
Cuts at: 9499 9533 1903
Size: 34 3678
Fragments arranged by size:
6658 3678 646 84 72 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9200 10410 9200
Size: 1210 10098
BclI T'GATC_A
Cuts at: 1548 4464 1548
Size: 2916 8392
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2820 3750
Size: 107 122 71 631 605 337 811 930
Cuts at: 3750 4127 7413 9919 136
Size: 377 3286 2506 1525
Fragments arranged by size:
3286 2506 1525 930 811 631 605 377
337 122 107 71
BglII A'GATC_T
Cuts at: 11303 11303
Size: 11308
BlpI GC'TnA_GC
Cuts at: 2372 8725 2372
Size: 6353 4955
Bme1580I G_kGCm'C
Cuts at: 1790 2769 4382 7201 7242 7623 7887 9369 10525
Size: 979 1613 2819 41 381 264 1482 1156
Cuts at: 10525 1790
Size: 2573
Fragments arranged by size:
2819 2573 1613 1482 1156 979 381 264
41
BmgBI CAC'GTC
Cuts at: 1318 4528 4706 1318
Size: 3210 178 7920
Fragments arranged by size:
7920 3210 178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2185 2756 3303
Size: 416 53 83 399 24 768 571 547
Cuts at: 3303 3690 3783 9039 9997 442
Size: 387 93 5256 958 1753
Fragments arranged by size:
5256 1753 958 768 571 547 416 399
387 93 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2228 2402 2411 2432 3222 4070 4112 4818
Size: 989 174 9 21 790 848 42 706
Cuts at: 4818 5550 6461 6776 7213 9968 1239
Size: 732 911 315 437 2755 2579
Fragments arranged by size:
2755 2579 989 911 848 790 732 706
437 315 174 42 21 9
Bpu10I CC'TnA_GC
Cuts at: 4812 5229 5478 6297 7838 8025 4812
Size: 417 249 819 1541 187 8095
Fragments arranged by size:
8095 1541 819 417 249 187
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 3206 5390 6856 9433 10298 10596 2063
Size: 1143 2184 1466 2577 865 298 2775
Fragments arranged by size:
2775 2577 2184 1466 1143 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4292 4530 4658 4991 6395 6805 7046
Size: 617 2793 238 128 333 1404 410 241
Cuts at: 7046 8557 9971 882
Size: 1511 1414 2219
Fragments arranged by size:
2793 2219 1511 1414 1404 617 410 333
241 238 128
BsaAI yAC'GTr
Cuts at: 493 1204 4134 9099 493
Size: 711 2930 4965 2702
Fragments arranged by size:
4965 2930 2702 711
BsaBI GATnn'nnATC
Cuts at: 11302 11302
Size: 11308
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4028 9115 9497 10132
Size: 53 83 186 936 2495 5087 382 635
Cuts at: 10132 275
Size: 1451
Fragments arranged by size:
5087 2495 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4242 6549 6865 7224 7748 9741 10139 10413 10560
Size: 2307 316 359 524 1993 398 274 147
Cuts at: 10560 10971 4242
Size: 411 4579
Fragments arranged by size:
4579 2307 1993 524 411 398 359 316
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2882 2912 3068 3098 3209
Size: 30 165 30 1479 30 156 30 111
Cuts at: 3209 3239 4562 4592 5502 5532 1178
Size: 30 1323 30 910 30 6954
Fragments arranged by size:
6954 1479 1323 910 165 156 111 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2399 2792 3089 3867 4303 5780 7906 1186
Size: 1213 393 297 778 436 1477 2126 4588
Fragments arranged by size:
4588 2126 1477 1213 778 436 393 297
BseYI C'CCAG_C
Cuts at: 2252 2669 4421 4654 5262 5535 5808 6052 6081
Size: 417 1752 233 608 273 273 244 29
Cuts at: 6081 6542 10511 11007 2252
Size: 461 3969 496 2553
Fragments arranged by size:
3969 2553 1752 608 496 461 417 273
273 244 233 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3606 4128 4337 5111 5348 5621 5966
Size: 573 1870 522 209 774 237 273 345
Cuts at: 5966 6167 1163
Size: 201 6304
Fragments arranged by size:
6304 1870 774 573 522 345 273 237
209 201
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2494 2968 3439 8758 9519 10547
Size: 275 186 493 474 471 5319 761 1028
Cuts at: 10547 1540
Size: 2301
Fragments arranged by size:
5319 2301 1028 761 493 474 471 275
186
BsiHKAI G_wGCw'C
Cuts at: 2274 2784 2943 3222 5313 5472 6132 7634 8220
Size: 510 159 279 2091 159 660 1502 586
Cuts at: 8220 8416 9369 9454 10525 2274
Size: 196 953 85 1071 3057
Fragments arranged by size:
3057 2091 1502 1071 953 660 586 510
279 196 159 159 85
BsmI GAATG_Cn'
Cuts at: 4107 6670 8819 8912 4107
Size: 2563 2149 93 6503
Fragments arranged by size:
6503 2563 2149 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3823 3908 8080 3823
Size: 85 4172 7051
Fragments arranged by size:
7051 4172 85
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2274 2568 2769 2784 2943 3222
Size: 399 463 21 294 201 15 159 279
Cuts at: 3222 4382 5313 5472 5679 6132 7139 7201 7242
Size: 1160 931 159 207 453 1007 62 41
Cuts at: 7242 7623 7634 7844 7887 8220 8416 8627 9369
Size: 381 11 210 43 333 196 211 742
Cuts at: 9369 9454 10525 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1160 1071 1007 931 742 463 453
399 381 333 294 279 211 210 207
201 196 159 159 85 62 43 41
21 15 11
BspEI T'CCGG_A
Cuts at: 4242 6549 7224 10971 4242
Size: 2307 675 3747 4579
Fragments arranged by size:
4579 3747 2307 675
BspHI T'CATG_A
Cuts at: 9197 10927 9197
Size: 1730 9578
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2021 2999 3047 4150 5676 7764 8238 8324
Size: 1177 978 48 1103 1526 2088 474 86
Cuts at: 8324 8663 844
Size: 339 3489
Fragments arranged by size:
3489 2088 1526 1177 1103 978 474 339
86 48
BsrBI CCG'CTC
Cuts at: 1394 1823 9195 1394
Size: 429 7372 3507
Fragments arranged by size:
7372 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2426 2686 4212 7470 7971 9803 9977
Size: 1183 1178 260 1526 3258 501 1832 174
Cuts at: 9977 65
Size: 1396
Fragments arranged by size:
3258 1832 1526 1396 1183 1178 501 260
174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2598 3066 3107 3572 4308 7748 9952
Size: 110 788 468 41 465 736 3440 2204
Cuts at: 9952 10139 1700
Size: 187 2869
Fragments arranged by size:
3440 2869 2204 788 736 468 465 187
110 41
BsrGI T'GTAC_A
Cuts at: 96 3969 96
Size: 3873 7435
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11308
BssSI C'ACGA_G
Cuts at: 3746 3983 9368 10380 3746
Size: 237 5385 1012 4674
Fragments arranged by size:
5385 4674 1012 237
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3675 8135 794
Size: 2881 4460 3967
Fragments arranged by size:
4460 3967 2881
BstBI TT'CG_AA
Cuts at: 2721 10112 2721
Size: 7391 3917
BstEII G'GTnAC_C
Cuts at: 1184 4396 7751 1184
Size: 3212 3355 4741
Fragments arranged by size:
4741 3355 3212
BstXI CCAn_nnnn'nTGG
Cuts at: 3989 4411 4957 5344 7399 3989
Size: 422 546 387 2055 7898
Fragments arranged by size:
7898 2055 546 422 387
BstYI r'GATC_y
Cuts at: 1972 2186 2203 4201 6040 6393 7866 8363 8638
Size: 214 17 1998 1839 353 1473 497 275
Cuts at: 8638 9005 9390 9407 10859 10945 10957 11303 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1998 1977 1839 1473 1452 497 385 367
353 346 275 214 86 17 17 12
Bsu36I CC'TnA_GG
Cuts at: 2411 4286 5951 6200 7889 2411
Size: 1875 1665 249 1689 5830
Fragments arranged by size:
5830 1875 1689 1665 249
BtgI C'CryG_G
Cuts at: 513 1065 2004 2875 3247 3613 5710 5934 7131
Size: 552 939 871 372 366 2097 224 1197
Cuts at: 7131 7263 7584 8239 8343 513
Size: 132 321 655 104 3478
Fragments arranged by size:
3478 2097 1197 939 871 655 552 372
366 321 224 132 104
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2294 2847 7327 487
Size: 683 56 1068 553 4480 4468
Fragments arranged by size:
4480 4468 1068 683 553 56
BtsI GCAGTG_nn'
Cuts at: 2621 2779 2941 3089 3247 3275 3406 3647 4455
Size: 158 162 148 158 28 131 241 808
Cuts at: 4455 7641 8824 9618 9638 2621
Size: 3186 1183 794 20 4291
Fragments arranged by size:
4291 3186 1183 808 794 241 162 158
158 148 131 28 20
ClaI AT'CG_AT
Cuts at: 1554 4494 4551 1554
Size: 2940 57 8311
Fragments arranged by size:
8311 2940 57
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11273
DraI TTT'AAA
Cuts at: 8612 8612
Size: 11308
DraIII CAC_nnn'GTG
Cuts at: 4360 4360
Size: 11308
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7490 10315 809
Size: 507 6174 2825 1802
Fragments arranged by size:
6174 2825 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3157 3244 3250
Size: 54 1425 50 275 18 1327 87 6
Cuts at: 3250 3436 3616 3709 3730 3772 4128 5031 5523
Size: 186 180 93 21 42 356 903 492
Cuts at: 5523 7452 8755 9644 11273 8
Size: 1929 1303 889 1629 43
Fragments arranged by size:
1929 1629 1425 1327 1303 903 889 492
356 275 186 180 93 87 54 50
43 42 21 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3436 8755 1537
Size: 275 1624 5319 4090
Fragments arranged by size:
5319 4090 1624 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2127 3122 3906 4097 4385 5889 7622 9238
Size: 774 995 784 191 288 1504 1733 1616
Cuts at: 9238 1353
Size: 3423
Fragments arranged by size:
3423 1733 1616 1504 995 784 774 288
191
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 9895 10269 10415 387
Size: 1064 138 8306 374 146 1280
Fragments arranged by size:
8306 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2941 3220 7632 8218 2941
Size: 279 4412 586 6031
Fragments arranged by size:
6031 4412 586 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2228 2229 2402 2411 2432 2488 2532
Size: 393 596 1 173 9 21 56 44
Cuts at: 2532 3222 4070 4112 4170 4409 4613 4818 4961
Size: 690 848 42 58 239 204 205 143
Cuts at: 4961 5550 5713 6461 6776 7213 8327 8560 9371
Size: 589 163 748 315 437 1114 233 811
Cuts at: 9371 9968 10755 11023 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1114 848 811 787 748 690 597
596 589 437 393 315 268 239 233
205 204 173 163 143 58 56 44
42 21 9 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2457 7377 7453 2457
Size: 4920 76 6312
Fragments arranged by size:
6312 4920 76
EcoO109I rG'GnC_Cy
Cuts at: 2074 2478 2549 3529 4378 4379 4972 5574 6586
Size: 404 71 980 849 1 593 602 1012
Cuts at: 6586 7619 7620 7884 2074
Size: 1033 1 264 5498
Fragments arranged by size:
5498 1033 1012 980 849 602 593 404
264 71 1 1
EcoRI G'AATT_C
Cuts at: 6725 8162 8617 8995 6725
Size: 1437 455 378 9038
Fragments arranged by size:
9038 1437 455 378
EcoRV GAT'ATC
Cuts at: 4347 4347
Size: 11308
FspI TGC'GCA
Cuts at: 9814 9814
Size: 11308
HaeII r_GCGC'y
Cuts at: 1678 4122 8033 10455 1678
Size: 2444 3911 2422 2531
Fragments arranged by size:
3911 2531 2444 2422
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2195 2227 2697 2729 3537
Size: 32 135 32 623 32 470 32 808
Cuts at: 3537 3569 3801 3833 4013 4045 6237 6269 6352
Size: 32 232 32 180 32 2192 32 83
Cuts at: 6352 6384 7394 7426 7685 7717 7802 7834 7970
Size: 32 1010 32 259 32 85 32 136
Cuts at: 7970 8002 8039 8071 9949 9981 10023 10055 1373
Size: 32 37 32 1878 32 42 32 2626
Fragments arranged by size:
2626 2192 1878 1010 808 623 470 259
232 180 136 135 85 83 42 37
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3922 6157 6874 8461 8506 8652
Size: 988 281 1984 2235 717 1587 45 146
Cuts at: 8652 8898 669
Size: 246 3079
Fragments arranged by size:
3079 2235 1984 1587 988 717 281 246
146 45
HindIII A'AGCT_T
Cuts at: 748 2260 748
Size: 1512 9796
HpaI GTT'AAC
Cuts at: 8506 8898 8506
Size: 392 10916
KpnI G_GTAC'C
Cuts at: 8633 8633
Size: 11308
MfeI C'AATT_G
Cuts at: 2863 7923 8907 2863
Size: 5060 984 5264
Fragments arranged by size:
5264 5060 984
MluI A'CGCG_T
Cuts at: 11184 11184
Size: 11308
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4938 6642 7683 10422 10606 1305
Size: 3633 1704 1041 2739 184 2007
Fragments arranged by size:
3633 2739 2007 1704 1041 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3246 3711 3774 4130 5033
Size: 54 1425 343 1414 465 63 356 903
Cuts at: 5033 7454 10
Size: 2421 3864
Fragments arranged by size:
3864 2421 1425 1414 903 465 356 343
63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3153 3987 5324 5597 6143 7346 9266
Size: 648 1987 834 1337 273 546 1203 1920
Cuts at: 9266 9625 9784 518
Size: 359 159 2042
Fragments arranged by size:
2042 1987 1920 1337 1203 834 648 546
359 273 159
MspA1I CmG'CkG
Cuts at: 1606 2023 2659 2976 3162 3255 3348 3441 3720
Size: 417 636 317 186 93 93 93 279
Cuts at: 3720 3828 3852 5294 5712 6052 6113 6929 7191
Size: 108 24 1442 418 340 61 816 262
Cuts at: 7191 8724 9401 10549 10794 1606
Size: 1533 677 1148 245 2120
Fragments arranged by size:
2120 1533 1442 1148 816 677 636 418
417 340 317 279 262 245 186 108
93 93 93 61 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 3247 3613 5934 7131 7263 8239
Size: 552 939 1243 366 2321 1197 132 976
Cuts at: 8239 8343 513
Size: 104 3478
Fragments arranged by size:
3478 2321 1243 1197 976 939 552 366
132 104
NdeI CA'TA_TG
Cuts at: 387 8131 387
Size: 7744 3564
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11308
NotI GC'GGCC_GC
Cuts at: 8755 8755
Size: 11308
NspI r_CATG'y
Cuts at: 1132 1229 2793 3050 3457 4645 5204 8666 10211
Size: 97 1564 257 407 1188 559 3462 1545
Cuts at: 10211 1132
Size: 2229
Fragments arranged by size:
3462 2229 1564 1545 1188 559 407 257
97
PasI CC'CwG_GG
Cuts at: 1348 3061 3510 8203 1348
Size: 1713 449 4693 4453
Fragments arranged by size:
4693 4453 1713 449
PciI A'CATG_T
Cuts at: 2789 4641 5200 10207 2789
Size: 1852 559 5007 3890
Fragments arranged by size:
5007 3890 1852 559
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2146 5083 1108
Size: 1038 2937 7333
Fragments arranged by size:
7333 2937 1038
PfoI T'CCnGG_A
Cuts at: 4041 4089 5874 6374 6647 6920 7541 4041
Size: 48 1785 500 273 273 621 7808
Fragments arranged by size:
7808 1785 621 500 273 273 48
PmeI GTTT'AAAC
Cuts at: 8612 8612
Size: 11308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4562 4594 7273 7305 9377 9409 10890 10922 4562
Size: 32 2679 32 2072 32 1481 32 4948
Fragments arranged by size:
4948 2679 2072 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2074 4972 2074
Size: 2898 8410
PshAI GACnn'nnGTC
Cuts at: 1613 5656 1613
Size: 4043 7265
PsiI TTA'TAA
Cuts at: 8878 8878
Size: 11308
PspOMI G'GGCC_C
Cuts at: 4378 7619 7883 4378
Size: 3241 264 7803
Fragments arranged by size:
7803 3241 264
PspXI vC'TCGA_Gb
Cuts at: 6224 7574 6224
Size: 1350 9958
PstI C_TGCA'G
Cuts at: 830 2431 2654 2747 2774 2936 3122 3242 3308
Size: 1601 223 93 27 162 186 120 66
Cuts at: 3308 3401 3521 4996 8660 830
Size: 93 120 1475 3664 3478
Fragments arranged by size:
3664 3478 1601 1475 223 186 162 120
120 93 93 66 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11308
PvuII CAG'CTG
Cuts at: 2023 2659 5294 6052 6113 6929 7191 2023
Size: 636 2635 758 61 816 262 6140
Fragments arranged by size:
6140 2635 816 758 636 262 61
SacI G_AGCT'C
Cuts at: 2943 3222 7634 8220 2943
Size: 279 4412 586 6031
Fragments arranged by size:
6031 4412 586 279
SacII CC_GC'GG
Cuts at: 5713 5713
Size: 11308
SalI G'TCGA_C
Cuts at: 1936 8650 1936
Size: 6714 4594
SapI GCTCTTCn'nnn_
Cuts at: 4097 7622 4097
Size: 3525 7783
SbfI CC_TGCA'GG
Cuts at: 8660 8660
Size: 11308
ScaI AGT'ACT
Cuts at: 4067 4577 5585 6288 6508 9556 11268 4067
Size: 510 1008 703 220 3048 1712 4107
Fragments arranged by size:
4107 3048 1712 1008 703 510 220
SexAI A'CCwGG_T
Cuts at: 2397 4469 5886 2397
Size: 2072 1417 7819
Fragments arranged by size:
7819 2072 1417
SfcI C'TryA_G
Cuts at: 826 1045 2427 2485 2650 2743 2770 2932 3118
Size: 219 1382 58 165 93 27 162 186
Cuts at: 3118 3238 3304 3397 3517 3847 4992 5455 6021
Size: 120 66 93 120 330 1145 463 566
Cuts at: 6021 6274 7116 7822 8656 9791 10472 10663 826
Size: 253 842 706 834 1135 681 191 1471
Fragments arranged by size:
1471 1382 1145 1135 842 834 706 681
566 463 330 253 219 191 186 165
162 120 120 93 93 66 58 27
SfiI GGCCn_nnn'nGGCC
Cuts at: 7413 7413
Size: 11308
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11308
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11308
SmaI CCC'GGG
Cuts at: 1872 4376 6219 7736 8635 1872
Size: 2504 1843 1517 899 4545
Fragments arranged by size:
4545 2504 1843 1517 899
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 3221 5405 6224 6835 7574
Size: 197 926 135 1143 2184 819 611 739
Cuts at: 7574 9412 10313 10575 820
Size: 1838 901 262 1553
Fragments arranged by size:
2184 1838 1553 1143 926 901 819 739
611 262 197 135
SnaBI TAC'GTA
Cuts at: 493 9099 493
Size: 8606 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11308
SphI G_CATG'C
Cuts at: 1132 8666 1132
Size: 7534 3774
SspI AAT'ATT
Cuts at: 5 52 9232 5
Size: 47 9180 2081
Fragments arranged by size:
9180 2081 47
StuI AGG'CCT
Cuts at: 1517 3762 4155 4680 5130 5303 5483 5949 6302
Size: 2245 393 525 450 173 180 466 353
Cuts at: 6302 6768 1517
Size: 466 6057
Fragments arranged by size:
6057 2245 525 466 466 450 393 353
180 173
StyI C'CwwG_G
Cuts at: 513 1065 2004 3247 3613 5934 6814 7131 7263
Size: 552 939 1243 366 2321 880 317 132
Cuts at: 7263 7449 7947 8239 8343 8747 513
Size: 186 498 292 104 404 3074
Fragments arranged by size:
3074 2321 1243 939 880 552 498 404
366 317 292 186 132 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3074 3995 5071 5813 6827 7216 7583
Size: 1255 1494 921 1076 742 1014 389 367
Cuts at: 7583 7865 9335 9352 9505 9690 10135 325
Size: 282 1470 17 153 185 445 1498
Fragments arranged by size:
1498 1494 1470 1255 1076 1014 921 742
445 389 367 282 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2423 3969 4065
Size: 274 80 33 51 1443 446 1546 96
Cuts at: 4065 4575 5148 5583 6286 6506 9554 11266 96
Size: 510 573 435 703 220 3048 1712 138
Fragments arranged by size:
3048 1712 1546 1443 703 573 510 446
435 274 220 138 96 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2871 2882 2903 2914 3068 3100 3209 3241 3533
Size: 11 21 11 154 32 109 32 292
Cuts at: 3533 3565 3719 3751 4181 4213 5502 5534 8061
Size: 32 154 32 430 32 1289 32 2527
Cuts at: 8061 8093 8320 8352 11268 11300 2871
Size: 32 227 32 2916 32 2879
Fragments arranged by size:
2916 2879 2527 1289 430 292 227 154
154 109 32 32 32 32 32 32
32 32 32 21 11 11
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11308
XbaI T'CTAG_A
Cuts at: 4207 8644 4207
Size: 4437 6871
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2464 4408 6436 7210 7270 7477 7877 2464
Size: 1944 2028 774 60 207 400 5895
Fragments arranged by size:
5895 2028 1944 774 400 207 60
XhoI C'TCGA_G
Cuts at: 1943 6224 7574 1943
Size: 4281 1350 5677
Fragments arranged by size:
5677 4281 1350
XmaI C'CCGG_G
Cuts at: 1870 4374 6217 7734 8633 1870
Size: 2504 1843 1517 899 4545
Fragments arranged by size:
4545 2504 1843 1517 899
XmnI GAAnn'nnTTC
Cuts at: 2262 9437 2262
Size: 7175 4133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4029 9116 276
Size: 53 83 186 936 2495 5087 2468
Fragments arranged by size:
5087 2495 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BbeI BfrBI BplI BsiWI BstZ17I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI RsrII SanDI SfoI SrfI
SwaI