Restriction map for
pFN21AB0054
(Circular) (Six-base) MAPSORT of: pFN21AB0054.seq Check: 112 from: 1 to: 7813
pFN21AB0054 7813 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5623 278
Size: 53 83 186 936 4087 2468
Fragments arranged by size:
4087 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2778 4216 4503 5156 1937
Size: 841 1438 287 653 4594
Fragments arranged by size:
4594 1438 841 653 287
Acc65I G'GTAC_C
Cuts at: 5134 5134
Size: 7813
AclI AA'CG_TT
Cuts at: 1563 5940 6313 1563
Size: 4377 373 3063
Fragments arranged by size:
4377 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2408 2925 3514 4053 4139 4589 4964 5876
Size: 776 517 589 539 86 450 375 912
Cuts at: 5876 7260 7528 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 912 776 589 539 517 450
375 268 86
AflII C'TTAA_G
Cuts at: 820 1017 3347 820
Size: 197 2330 5286
Fragments arranged by size:
5286 2330 197
AflIII A'CryG_T
Cuts at: 1203 3230 4705 6712 7689 1203
Size: 2027 1475 2007 977 1327
Fragments arranged by size:
2027 2007 1475 1327 977
AgeI A'CCGG_T
Cuts at: 6644 6644
Size: 7813
AhdI GACnn_n'nnGTC
Cuts at: 6542 6542
Size: 7813
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7813
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7813
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4425 4457 4425
Size: 32 7781
AlwNI CAG_nnn'CTG
Cuts at: 5556 7128 5556
Size: 1572 6241
ApaLI G'TGCA_C
Cuts at: 2756 5870 7026 2756
Size: 3114 1156 3543
Fragments arranged by size:
3543 3114 1156
ApoI r'AATT_y
Cuts at: 1493 2254 2592 3070 3728 3870 5122 5348 5500
Size: 761 338 478 658 142 1252 226 152
Cuts at: 5500 7609 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1252 761 658 478 338 226
152 142
AseI AT'TA_AT
Cuts at: 160 1032 3246 3579 4371 6367 160
Size: 872 2214 333 792 1996 1606
Fragments arranged by size:
2214 1996 1606 872 792 333
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
AvaI C'yCGr_G
Cuts at: 1870 1943 4833 5138 1870
Size: 73 2890 305 4545
Fragments arranged by size:
4545 2890 305 73
BamHI G'GATC_C
Cuts at: 5143 5510 5143
Size: 367 7446
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5134 6589 618
Size: 325 217 627 3347 1455 1842
Fragments arranged by size:
3347 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3758 5132 1391
Size: 2367 1374 4072
Fragments arranged by size:
4072 2367 1374
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2004 3034 4911 928
Size: 971 105 1030 1877 3830
Fragments arranged by size:
3830 1877 1030 971 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6004 6038 1903
Size: 34 4067 34 3678
Fragments arranged by size:
4067 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3959 5705 6915 3959
Size: 1746 1210 4857
Fragments arranged by size:
4857 1746 1210
BclI T'GATC_A
Cuts at: 1548 4012 4093 1548
Size: 2464 81 5268
Fragments arranged by size:
5268 2464 81
BfrBI ATG'CAT
Cuts at: 2318 3121 2318
Size: 803 7010
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6424 136
Size: 107 122 71 631 605 4752 1525
Fragments arranged by size:
4752 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2192 2800 7808 2192
Size: 608 5008 2197
Fragments arranged by size:
5008 2197 608
BlpI GC'TnA_GC
Cuts at: 4041 5230 4041
Size: 1189 6624
Bme1580I G_kGCm'C
Cuts at: 1790 2728 2760 4158 4764 5874 7030 1790
Size: 938 32 1398 606 1110 1156 2573
Fragments arranged by size:
2573 1398 1156 1110 938 606 32
BmgBI CAC'GTC
Cuts at: 1318 4708 1318
Size: 3390 4423
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5544 6502 442
Size: 416 53 83 399 24 4127 958 1753
Fragments arranged by size:
4127 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7813
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2137 4452 6473 1239
Size: 898 2315 2021 2579
Fragments arranged by size:
2579 2315 2021 898
Bpu10I CC'TnA_GC
Cuts at: 3752 4861 3752
Size: 1109 6704
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4892 5938 6803 7101 4892
Size: 1046 865 298 5604
Fragments arranged by size:
5604 1046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6476 882
Size: 617 4977 2219
Fragments arranged by size:
4977 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5604 493
Size: 711 4400 2702
Fragments arranged by size:
4400 2702 711
BsaBI GATnn'nnATC
Cuts at: 7807 7807
Size: 7813
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 5620 6002 6637
Size: 53 83 186 936 476 3611 382 635
Cuts at: 6637 275
Size: 1451
Fragments arranged by size:
3611 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6246 6644 6918 7065 7476 6246
Size: 398 274 147 411 6583
Fragments arranged by size:
6583 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4425 4455 1178
Size: 30 165 30 3022 30 4536
Fragments arranged by size:
4536 3022 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4272 4451 1186
Size: 3086 179 4548
Fragments arranged by size:
4548 3086 179
BseYI C'CCAG_C
Cuts at: 3961 4848 4921 7016 7512 3961
Size: 887 73 2095 496 4262
Fragments arranged by size:
4262 2095 887 496 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7240
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5263 6024 7052 1540
Size: 275 186 3262 761 1028 2301
Fragments arranged by size:
3262 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2760 5874 5959 7030 2760
Size: 3114 85 1071 3543
Fragments arranged by size:
3543 3114 1071 85
BsmI GAATG_Cn'
Cuts at: 5324 5417 5324
Size: 93 7720
BsmBI CGTCTCn'nnnn_
Cuts at: 2330 2419 2522 2330
Size: 89 103 7621
Fragments arranged by size:
7621 103 89
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2728 2760 3758 4158 4764 5132 5874
Size: 399 938 32 998 400 606 368 742
Cuts at: 5874 5959 7030 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 998 938 742 606 400 399
368 85 32
BspEI T'CCGG_A
Cuts at: 7476 7476
Size: 7813
BspHI T'CATG_A
Cuts at: 5702 7432 5702
Size: 1730 6083
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2478 5168 844
Size: 1634 2690 3489
Fragments arranged by size:
3489 2690 1634
BsrBI CCG'CTC
Cuts at: 1394 1823 5026 5700 1394
Size: 429 3203 674 3507
Fragments arranged by size:
3507 3203 674 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2318 3385 3841 3960 4396 6308 6482
Size: 1183 1070 1067 456 119 436 1912 174
Cuts at: 6482 65
Size: 1396
Fragments arranged by size:
1912 1396 1183 1070 1067 456 436 174
119
BsrFI r'CCGG_y
Cuts at: 1700 1810 6457 6644 1700
Size: 110 4647 187 2869
Fragments arranged by size:
4647 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2268 96
Size: 2172 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7813
BssSI C'ACGA_G
Cuts at: 5873 6885 5873
Size: 1012 6801
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3564 794
Size: 2770 5043
BstBI TT'CG_AA
Cuts at: 2252 3139 6617 2252
Size: 887 3478 3448
Fragments arranged by size:
3478 3448 887
BstEII G'GTnAC_C
Cuts at: 1184 4482 1184
Size: 3298 4515
BstXI CCAn_nnnn'nTGG
Cuts at: 3866 4774 3866
Size: 908 6905
BstYI r'GATC_y
Cuts at: 1972 2192 2800 5143 5510 5895 5912 7364 7450
Size: 220 608 2343 367 385 17 1452 86
Cuts at: 7450 7462 7808 1972
Size: 12 346 1977
Fragments arranged by size:
2343 1977 1452 608 385 367 346 220
86 17 12
BstZ17I GTA'TAC
Cuts at: 2779 4504 2779
Size: 1725 6088
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 4800 513
Size: 552 939 12 2784 3526
Fragments arranged by size:
3526 2784 939 552 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7074
Fragments arranged by size:
7074 683 56
BtsI GCAGTG_nn'
Cuts at: 2101 2461 2757 2902 3227 5329 6123 6143 2101
Size: 360 296 145 325 2102 794 20 3771
Fragments arranged by size:
3771 2102 794 360 325 296 145 20
ClaI AT'CG_AT
Cuts at: 1554 2343 4137 1554
Size: 789 1794 5230
Fragments arranged by size:
5230 1794 789
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7778
DraI TTT'AAA
Cuts at: 5117 5117
Size: 7813
DraIII CAC_nnn'GTG
Cuts at: 2752 2752
Size: 7813
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6820 809
Size: 507 5504 1802
Fragments arranged by size:
5504 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 5260 6149
Size: 54 1425 50 275 18 198 3232 889
Cuts at: 6149 7778 8
Size: 1629 43
Fragments arranged by size:
3232 1629 1425 889 275 198 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5260 1537
Size: 275 3448 4090
Fragments arranged by size:
4090 3448 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2427 3000 5743 1353
Size: 1074 573 2743 3423
Fragments arranged by size:
3423 2743 1074 573
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6400 6774 6920 387
Size: 1064 138 4811 374 146 1280
Fragments arranged by size:
4811 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2137 2408 2925 3514 4053 4139 4452
Size: 393 505 271 517 589 539 86 313
Cuts at: 4452 4589 4964 5876 6473 7260 7528 1239
Size: 137 375 912 597 787 268 1524
Fragments arranged by size:
1524 912 787 597 589 539 517 505
393 375 313 271 268 137 86
EcoNI CCTnn'n_nnAGG
Cuts at: 4254 4254
Size: 7813
EcoO109I rG'GnC_Cy
Cuts at: 4794 4794
Size: 7813
EcoRI G'AATT_C
Cuts at: 3870 5122 5500 3870
Size: 1252 378 6183
Fragments arranged by size:
6183 1252 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4223 4223
Size: 7813
FspI TGC'GCA
Cuts at: 6319 6319
Size: 7813
HaeII r_GCGC'y
Cuts at: 1678 3912 6960 1678
Size: 2234 3048 2531
Fragments arranged by size:
3048 2531 2234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2728 2760 3152 3184 4130
Size: 32 135 32 1156 32 392 32 946
Cuts at: 4130 4162 4231 4263 6454 6486 6528 6560 1373
Size: 32 69 32 2191 32 42 32 2626
Fragments arranged by size:
2626 2191 1156 946 392 135 69 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5157 5403 669
Size: 988 281 3219 246 3079
Fragments arranged by size:
3219 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2244 2406 748
Size: 1496 162 6155
Fragments arranged by size:
6155 1496 162
HpaI GTT'AAC
Cuts at: 5403 5403
Size: 7813
KpnI G_GTAC'C
Cuts at: 5138 5138
Size: 7813
MfeI C'AATT_G
Cuts at: 2298 2523 5412 2298
Size: 225 2889 4699
Fragments arranged by size:
4699 2889 225
MluI A'CGCG_T
Cuts at: 3230 7689 3230
Size: 4459 3354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3687 6927 7111 1305
Size: 2382 3240 184 2007
Fragments arranged by size:
3240 2382 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5991
Fragments arranged by size:
5991 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3864 4161 4772 4913 5771 6130 6289
Size: 648 2698 297 611 141 858 359 159
Cuts at: 6289 518
Size: 2042
Fragments arranged by size:
2698 2042 858 648 611 359 297 159
141
MspA1I CmG'CkG
Cuts at: 1606 2093 2381 3848 4031 4723 5060 5229 5906
Size: 487 288 1467 183 692 337 169 677
Cuts at: 5906 7054 7299 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1467 1148 692 677 487 337 288
245 183 169
NcoI C'CATG_G
Cuts at: 513 1065 2004 4800 513
Size: 552 939 2796 3526
Fragments arranged by size:
3526 2796 939 552
NdeI CA'TA_TG
Cuts at: 387 3601 387
Size: 3214 4599
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7813
NotI GC'GGCC_GC
Cuts at: 5260 5260
Size: 7813
NsiI A_TGCA'T
Cuts at: 2320 3123 2320
Size: 803 7010
NspI r_CATG'y
Cuts at: 1132 1229 2110 2318 2511 3715 5171 6716 1132
Size: 97 881 208 193 1204 1456 1545 2229
Fragments arranged by size:
2229 1545 1456 1204 881 208 193 97
PasI CC'CwG_GG
Cuts at: 1348 2482 3971 1348
Size: 1134 1489 5190
Fragments arranged by size:
5190 1489 1134
PciI A'CATG_T
Cuts at: 6712 6712
Size: 7813
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3764 3836 1108
Size: 2656 72 5085
Fragments arranged by size:
5085 2656 72
PmeI GTTT'AAAC
Cuts at: 5117 5117
Size: 7813
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4425 4457 5882 5914 7395 7427 4425
Size: 32 1425 32 1481 32 4811
Fragments arranged by size:
4811 1481 1425 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4794 4794
Size: 7813
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7813
PsiI TTA'TAA
Cuts at: 2922 3126 5383 2922
Size: 204 2257 5352
Fragments arranged by size:
5352 2257 204
PstI C_TGCA'G
Cuts at: 830 3090 3108 3396 5165 830
Size: 2260 18 288 1769 3478
Fragments arranged by size:
3478 2260 1769 288 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7813
PvuII CAG'CTG
Cuts at: 2093 3848 4031 4723 5060 2093
Size: 1755 183 692 337 4846
Fragments arranged by size:
4846 1755 692 337 183
SalI G'TCGA_C
Cuts at: 1936 5155 1936
Size: 3219 4594
SanDI GG'GwC_CC
Cuts at: 4794 4794
Size: 7813
SapI GCTCTTCn'nnn_
Cuts at: 2427 2427
Size: 7813
SbfI CC_TGCA'GG
Cuts at: 5165 5165
Size: 7813
ScaI AGT'ACT
Cuts at: 6061 7773 6061
Size: 1712 6101
SfcI C'TryA_G
Cuts at: 826 1045 2818 3086 3104 3392 5036 5161 6296
Size: 219 1773 268 18 288 1644 125 1135
Cuts at: 6296 6977 7168 826
Size: 681 191 1471
Fragments arranged by size:
1773 1644 1471 1135 681 288 268 219
191 125 18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7813
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7813
SmaI CCC'GGG
Cuts at: 1872 5140 1872
Size: 3268 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3347 4833 4871 5917 6818 7080
Size: 197 926 1404 1486 38 1046 901 262
Cuts at: 7080 820
Size: 1553
Fragments arranged by size:
1553 1486 1404 1046 926 901 262 197
38
SnaBI TAC'GTA
Cuts at: 493 5604 493
Size: 5111 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7813
SphI G_CATG'C
Cuts at: 1132 2110 3715 5171 1132
Size: 978 1605 1456 3774
Fragments arranged by size:
3774 1605 1456 978
SspI AAT'ATT
Cuts at: 5 52 3307 4630 5737 5
Size: 47 3255 1323 1107 2081
Fragments arranged by size:
3255 2081 1323 1107 47
StuI AGG'CCT
Cuts at: 1517 2793 1517
Size: 1276 6537
StyI C'CwwG_G
Cuts at: 513 1065 2004 2370 2502 4800 5252 513
Size: 552 939 366 132 2298 452 3074
Fragments arranged by size:
3074 2298 939 552 452 366 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2963 5840 5857 6010 6195 6640 325
Size: 1255 1383 2877 17 153 185 445 1498
Fragments arranged by size:
2877 1498 1383 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2268 2401 2427
Size: 274 80 33 51 1443 291 133 26
Cuts at: 2427 2442 3082 4588 6059 7771 96
Size: 15 640 1506 1471 1712 138
Fragments arranged by size:
1712 1506 1471 1443 640 291 274 138
133 80 51 33 26 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3967 3999 7773 7805 3967
Size: 32 3774 32 3975
Fragments arranged by size:
3975 3774 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7813
XbaI T'CTAG_A
Cuts at: 4933 5149 4933
Size: 216 7597
XhoI C'TCGA_G
Cuts at: 1943 4833 1943
Size: 2890 4923
XmaI C'CCGG_G
Cuts at: 1870 5138 1870
Size: 3268 4545
XmnI GAAnn'nnTTC
Cuts at: 5942 5942
Size: 7813
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5621 276
Size: 53 83 186 936 4087 2468
Fragments arranged by size:
4087 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI Bsu36I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI
SrfI SwaI XcmI