Restriction map for
pFN21AB0053
(Circular) (Six-base) MAPSORT of: pFN21AB0053.seq Check: 8099 from: 1 to: 8641
pFN21AB0053 8641 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6451 278
Size: 53 83 186 936 4915 2468
Fragments arranged by size:
4915 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3099 5984 1937
Size: 1162 2885 4594
Fragments arranged by size:
4594 2885 1162
Acc65I G'GTAC_C
Cuts at: 5962 5962
Size: 8641
AclI AA'CG_TT
Cuts at: 1563 6768 7141 1563
Size: 5205 373 3063
Fragments arranged by size:
5205 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2658 2927 4167 4572 4944 5274 5781 6704
Size: 1026 269 1240 405 372 330 507 923
Cuts at: 6704 8088 8356 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1240 1026 923 507 405 372
330 269 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8444
AflIII A'CryG_T
Cuts at: 1203 7540 8517 1203
Size: 6337 977 1327
Fragments arranged by size:
6337 1327 977
AgeI A'CCGG_T
Cuts at: 7472 7472
Size: 8641
AhdI GACnn_n'nnGTC
Cuts at: 5248 7370 5248
Size: 2122 6519
AleI CACnn'nnGTG
Cuts at: 1166 2048 1166
Size: 882 7759
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8641
AlwNI CAG_nnn'CTG
Cuts at: 4844 5105 5870 6384 7956 4844
Size: 261 765 514 1572 5529
Fragments arranged by size:
5529 1572 765 514 261
ApaI G_GGCC'C
Cuts at: 2180 2180
Size: 8641
ApaLI G'TGCA_C
Cuts at: 6698 7854 6698
Size: 1156 7485
ApoI r'AATT_y
Cuts at: 1493 2666 2765 2957 3013 3127 3212 3424 5033
Size: 1173 99 192 56 114 85 212 1609
Cuts at: 5033 5950 6176 6328 8437 1493
Size: 917 226 152 2109 1697
Fragments arranged by size:
2109 1697 1609 1173 917 226 212 192
152 114 99 85 56
AseI AT'TA_AT
Cuts at: 160 1032 7195 160
Size: 872 6163 1606
Fragments arranged by size:
6163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8641
AvaI C'yCGr_G
Cuts at: 1870 1943 5966 1870
Size: 73 4023 4545
Fragments arranged by size:
4545 4023 73
BamHI G'GATC_C
Cuts at: 5605 5971 6338 5605
Size: 366 367 7908
Fragments arranged by size:
7908 367 366
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5962 7417 618
Size: 325 217 627 4175 1455 1842
Fragments arranged by size:
4175 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2180 5238 5297 5960 1391
Size: 789 3058 59 663 4072
Fragments arranged by size:
4072 3058 789 663 59
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2306 2457 2807 3495 4155 5124 928
Size: 971 407 151 350 688 660 969 4445
Fragments arranged by size:
4445 971 969 688 660 407 350 151
BbvCI CC'TCA_GC
Cuts at: 3564 3564
Size: 8641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6832 6866 1903
Size: 34 4895 34 3678
Fragments arranged by size:
4895 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3134 4051 6533 7743 3134
Size: 917 2482 1210 4032
Fragments arranged by size:
4032 2482 1210 917
BclI T'GATC_A
Cuts at: 1548 3454 4219 1548
Size: 1906 765 5970
Fragments arranged by size:
5970 1906 765
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 7252 136
Size: 107 122 71 631 605 337 5243 1525
Fragments arranged by size:
5243 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 2438 3106 8636 2438
Size: 668 5530 2443
Fragments arranged by size:
5530 2443 668
BlpI GC'TnA_GC
Cuts at: 6058 6058
Size: 8641
Bme1580I G_kGCm'C
Cuts at: 1790 2045 2180 6702 7858 1790
Size: 255 135 4522 1156 2573
Fragments arranged by size:
4522 2573 1156 255 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4938 5619 6372
Size: 416 53 83 399 24 3521 681 753
Cuts at: 6372 7330 442
Size: 958 1753
Fragments arranged by size:
3521 1753 958 753 681 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8641
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4194 4194
Size: 8641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2388 2461 3606 4323 4953 5231 5559 7301
Size: 1149 73 1145 717 630 278 328 1742
Cuts at: 7301 1239
Size: 2579
Fragments arranged by size:
2579 1742 1149 1145 717 630 328 278
73
Bpu10I CC'TnA_GC
Cuts at: 2388 3564 5370 2388
Size: 1176 1806 5659
Fragments arranged by size:
5659 1806 1176
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4164 5281 5600 6766 7631 7929 4164
Size: 1117 319 1166 865 298 4876
Fragments arranged by size:
4876 1166 1117 865 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7304 882
Size: 617 5805 2219
Fragments arranged by size:
5805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6432 493
Size: 711 5228 2702
Fragments arranged by size:
5228 2702 711
BsaBI GATnn'nnATC
Cuts at: 5604 5610 8635 5604
Size: 6 3025 5610
Fragments arranged by size:
5610 3025 6
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6448 6830 7465 275
Size: 53 83 186 936 4915 382 635 1451
Fragments arranged by size:
4915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 7074 7472 7746 7893 8304 7074
Size: 398 274 147 411 7411
Fragments arranged by size:
7411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2184 2214 3689 3719 5539
Size: 30 165 30 781 30 1475 30 1820
Cuts at: 5539 5557 5569 5587 5776 5806 1178
Size: 18 12 18 189 30 4013
Fragments arranged by size:
4013 1820 1475 781 189 165 30 30
30 30 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2082 2864 2867 3200 3344 3347 3650 3653
Size: 896 782 3 333 144 3 303 3
Cuts at: 3653 3656 3659 3710 4901 4916 4943 5391 5797
Size: 3 3 51 1191 15 27 448 406
Cuts at: 5797 5827 1186
Size: 30 4000
Fragments arranged by size:
4000 1191 896 782 448 406 333 303
144 51 30 27 15 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 5228 7844 8340 5228
Size: 2616 496 5529
Fragments arranged by size:
5529 2616 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2700 5956 1163
Size: 573 964 3256 3848
Fragments arranged by size:
3848 3256 964 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6091 6852 7880 1540
Size: 275 186 4090 761 1028 2301
Fragments arranged by size:
4090 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4180 5238 5297 6702 6787 7858 4180
Size: 1058 59 1405 85 1071 4963
Fragments arranged by size:
4963 1405 1071 1058 85 59
BsmI GAATG_Cn'
Cuts at: 6152 6245 6152
Size: 93 8548
BsmBI CGTCTCn'nnnn_
Cuts at: 2302 2302
Size: 8641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2180 4180 5238 5297 5960 6702
Size: 399 255 135 2000 1058 59 663 742
Cuts at: 6702 6787 7858 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 2000 1071 1058 742 663 399 255
135 85 59
BspEI T'CCGG_A
Cuts at: 8304 8304
Size: 8641
BspHI T'CATG_A
Cuts at: 6530 8260 6530
Size: 1730 6911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3892 5264 5996 844
Size: 3048 1372 732 3489
Fragments arranged by size:
3489 3048 1372 732
BsrBI CCG'CTC
Cuts at: 1394 1823 6528 1394
Size: 429 4705 3507
Fragments arranged by size:
4705 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3224 3257 7136 7310 65
Size: 1183 1976 33 3879 174 1396
Fragments arranged by size:
3879 1976 1396 1183 174 33
BsrFI r'CCGG_y
Cuts at: 1700 1810 5121 7285 7472 1700
Size: 110 3311 2164 187 2869
Fragments arranged by size:
3311 2869 2164 187 110
BsrGI T'GTAC_A
Cuts at: 96 2952 3043 5342 96
Size: 2856 91 2299 3395
Fragments arranged by size:
3395 2856 2299 91
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8641
BssSI C'ACGA_G
Cuts at: 6701 7713 6701
Size: 1012 7629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8641
BstBI TT'CG_AA
Cuts at: 5179 7445 5179
Size: 2266 6375
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8641
BstXI CCAn_nnnn'nTGG
Cuts at: 3110 3110
Size: 8641
BstYI r'GATC_y
Cuts at: 1972 2438 3106 4051 5605 5971 6338 6723 6740
Size: 466 668 945 1554 366 367 385 17
Cuts at: 6740 8192 8278 8290 8636 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1554 1452 945 668 466 385 367
366 346 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 8089
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7902
Fragments arranged by size:
7902 683 56
BtsI GCAGTG_nn'
Cuts at: 2712 2836 2896 4907 6157 6951 6971 2712
Size: 124 60 2011 1250 794 20 4382
Fragments arranged by size:
4382 2011 1250 794 124 60 20
ClaI AT'CG_AT
Cuts at: 1554 5632 1554
Size: 4078 4563
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5196 5231 529
Size: 35 4632 35 3939
Fragments arranged by size:
4632 3939 35 35
DraI TTT'AAA
Cuts at: 2739 4731 5796 5945 2739
Size: 1992 1065 149 5435
Fragments arranged by size:
5435 1992 1065 149
DraIII CAC_nnn'GTG
Cuts at: 4946 4946
Size: 8641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7648 809
Size: 507 6332 1802
Fragments arranged by size:
6332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6088 6977 8606
Size: 54 1425 50 275 18 4258 889 1629
Cuts at: 8606 8
Size: 43
Fragments arranged by size:
4258 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 6088 1537
Size: 275 4276 4090
Fragments arranged by size:
4276 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 2841 3627 3654 3687 4491 4527 4591
Size: 1101 387 786 27 33 804 36 64
Cuts at: 4591 4920 5166 5845 6571 1353
Size: 329 246 679 726 3423
Fragments arranged by size:
3423 1101 804 786 726 679 387 329
246 64 36 33 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7228 7602 7748 387
Size: 1064 138 5639 374 146 1280
Fragments arranged by size:
5639 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5236 5295 5236
Size: 59 8582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2388 2461 2658 2927 3606 4167 4323
Size: 393 756 73 197 269 679 561 156
Cuts at: 4323 4572 4944 4953 5231 5274 5559 5781 6704
Size: 249 372 9 278 43 285 222 923
Cuts at: 6704 7301 8088 8356 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 923 787 756 679 597 561 393
372 285 278 269 268 249 222 197
156 73 43 9
EcoO109I rG'GnC_Cy
Cuts at: 2011 2176 3808 2011
Size: 165 1632 6844
Fragments arranged by size:
6844 1632 165
EcoRI G'AATT_C
Cuts at: 5950 6328 5950
Size: 378 8263
EcoRV GAT'ATC
Cuts at: 3008 3008
Size: 8641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2764 4574 5738 2764
Size: 1810 1164 5667
Fragments arranged by size:
5667 1810 1164
FspI TGC'GCA
Cuts at: 7147 7147
Size: 8641
HaeII r_GCGC'y
Cuts at: 1678 3997 7788 1678
Size: 2319 3791 2531
Fragments arranged by size:
3791 2531 2319
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2447 2453 2479 2485 3898
Size: 32 135 32 875 6 26 6 1413
Cuts at: 3898 3930 4772 4804 5234 5266 5557 5589 7282
Size: 32 842 32 430 32 291 32 1693
Cuts at: 7282 7314 7356 7388 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1693 1413 875 842 430 291 135
42 32 32 32 32 32 32 32
32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 3244 3472 5880 5985 6231 669
Size: 988 281 1306 228 2408 105 246 3079
Fragments arranged by size:
3079 2408 1306 988 281 246 228 105
HindIII A'AGCT_T
Cuts at: 748 4128 748
Size: 3380 5261
HpaI GTT'AAC
Cuts at: 3244 3472 5880 6231 3244
Size: 228 2408 351 5654
Fragments arranged by size:
5654 2408 351 228
KpnI G_GTAC'C
Cuts at: 5966 5966
Size: 8641
MfeI C'AATT_G
Cuts at: 2948 5208 6240 2948
Size: 2260 1032 5349
Fragments arranged by size:
5349 2260 1032
MluI A'CGCG_T
Cuts at: 8517 8517
Size: 8641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5649 7755 7939 1305
Size: 4344 2106 184 2007
Fragments arranged by size:
4344 2106 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6819
Fragments arranged by size:
6819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2048 5531 6599 6958 7117 518
Size: 648 882 3483 1068 359 159 2042
Fragments arranged by size:
3483 2042 1068 882 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3134 5483 5867 6057 6734 7882 8127 1606
Size: 1528 2349 384 190 677 1148 245 2120
Fragments arranged by size:
2349 2120 1528 1148 677 384 245 190
NaeI GCC'GGC
Cuts at: 5123 5123
Size: 8641
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8089
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8641
NgoMIV G'CCGG_C
Cuts at: 5121 5121
Size: 8641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8641
NotI GC'GGCC_GC
Cuts at: 6088 6088
Size: 8641
NspI r_CATG'y
Cuts at: 1132 1229 5999 7544 1132
Size: 97 4770 1545 2229
Fragments arranged by size:
4770 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8641
PciI A'CATG_T
Cuts at: 7540 7540
Size: 8641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5045 1108
Size: 3937 4704
PfoI T'CCnGG_A
Cuts at: 5575 5575
Size: 8641
PmeI GTTT'AAAC
Cuts at: 5945 5945
Size: 8641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6710 6742 8223 8255 6710
Size: 32 1481 32 7096
Fragments arranged by size:
7096 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3808 3808
Size: 8641
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8641
PsiI TTA'TAA
Cuts at: 3162 6211 3162
Size: 3049 5592
PspOMI G'GGCC_C
Cuts at: 2176 2176
Size: 8641
PstI C_TGCA'G
Cuts at: 830 3139 4861 4914 5993 830
Size: 2309 1722 53 1079 3478
Fragments arranged by size:
3478 2309 1722 1079 53
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8641
PvuII CAG'CTG
Cuts at: 3134 5483 5867 3134
Size: 2349 384 5908
Fragments arranged by size:
5908 2349 384
SacI G_AGCT'C
Cuts at: 5238 5297 5238
Size: 59 8582
SalI G'TCGA_C
Cuts at: 1936 5983 1936
Size: 4047 4594
SapI GCTCTTCn'nnn_
Cuts at: 4591 4591
Size: 8641
SbfI CC_TGCA'GG
Cuts at: 5993 5993
Size: 8641
ScaI AGT'ACT
Cuts at: 3191 6889 8601 3191
Size: 3698 1712 3231
Fragments arranged by size:
3698 3231 1712
SfcI C'TryA_G
Cuts at: 826 1045 2274 3135 3193 4857 4910 5989 7124
Size: 219 1229 861 58 1664 53 1079 1135
Cuts at: 7124 7805 7996 826
Size: 681 191 1471
Fragments arranged by size:
1664 1471 1229 1135 1079 861 681 219
191 58 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8641
SmaI CCC'GGG
Cuts at: 1872 5968 1872
Size: 4096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4179 5296 5615 6745 7646 7908
Size: 197 926 2236 1117 319 1130 901 262
Cuts at: 7908 820
Size: 1553
Fragments arranged by size:
2236 1553 1130 1117 926 901 319 262
197
SnaBI TAC'GTA
Cuts at: 493 6432 493
Size: 5939 2702
SpeI A'CTAG_T
Cuts at: 152 2705 152
Size: 2553 6088
SphI G_CATG'C
Cuts at: 1132 5999 1132
Size: 4867 3774
SspI AAT'ATT
Cuts at: 5 52 2638 3070 3397 6565 5
Size: 47 2586 432 327 3168 2081
Fragments arranged by size:
3168 2586 2081 432 327 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 8641
StyI C'CwwG_G
Cuts at: 513 1065 6080 513
Size: 552 5015 3074
Fragments arranged by size:
5015 3074 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4957 6668 6685 6838 7023 7468 325
Size: 1255 3377 1711 17 153 185 445 1498
Fragments arranged by size:
3377 1711 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2952 2998 3043
Size: 274 80 33 51 1443 975 46 45
Cuts at: 3043 3189 5342 6887 8599 96
Size: 146 2153 1545 1712 138
Fragments arranged by size:
2153 1712 1545 1443 975 274 146 138
80 51 46 45 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2184 2216 3689 3721 3825 3857 8601 8633 2184
Size: 32 1473 32 104 32 4744 32 2192
Fragments arranged by size:
4744 2192 1473 104 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5569 1316
Size: 4253 4388
XbaI T'CTAG_A
Cuts at: 5977 5977
Size: 8641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3737 3737
Size: 8641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8641
XmaI C'CCGG_G
Cuts at: 1870 5966 1870
Size: 4096 4545
XmnI GAAnn'nnTTC
Cuts at: 2916 4749 5175 5721 6770 2916
Size: 1833 426 546 1049 4787
Fragments arranged by size:
4787 1833 1049 546 426
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6449 276
Size: 53 83 186 936 4915 2468
Fragments arranged by size:
4915 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BstZ17I Bsu36I EcoNI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI