Restriction map for
pFN21AB0051
(Circular) (Six-base) MAPSORT of: pFN21AB0051.seq Check: 7407 from: 1 to: 10078
pFN21AB0051 10078 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2209 2209
Size: 10078
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4788 7888 278
Size: 53 83 186 936 3252 3100 2468
Fragments arranged by size:
3252 3100 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5718 7421 1937
Size: 3781 1703 4594
Fragments arranged by size:
4594 3781 1703
Acc65I G'GTAC_C
Cuts at: 4179 7047 7399 4179
Size: 2868 352 6858
Fragments arranged by size:
6858 2868 352
AclI AA'CG_TT
Cuts at: 1563 8205 8578 1563
Size: 6642 373 3063
Fragments arranged by size:
6642 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2137 3140 3362 5441 6538 8141 9525 9793
Size: 505 1003 222 2079 1097 1603 1384 268
Cuts at: 9793 1632
Size: 1917
Fragments arranged by size:
2079 1917 1603 1384 1097 1003 505 268
222
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9881
AflIII A'CryG_T
Cuts at: 1203 2205 2274 2415 7071 7122 8977 9954 1203
Size: 1002 69 141 4656 51 1855 977 1327
Fragments arranged by size:
4656 1855 1327 1002 977 141 69 51
AgeI A'CCGG_T
Cuts at: 8909 8909
Size: 10078
AhdI GACnn_n'nnGTC
Cuts at: 2416 2646 6568 8807 2416
Size: 230 3922 2239 3687
Fragments arranged by size:
3922 3687 2239 230
AleI CACnn'nnGTG
Cuts at: 1166 3581 4382 6359 1166
Size: 2415 801 1977 4885
Fragments arranged by size:
4885 2415 1977 801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2319 5597 7280 1184
Size: 1135 3278 1683 3982
Fragments arranged by size:
3982 3278 1683 1135
AlwNI CAG_nnn'CTG
Cuts at: 3964 4024 4456 6991 7821 9393 3964
Size: 60 432 2535 830 1572 4649
Fragments arranged by size:
4649 2535 1572 830 432 60
ApaI G_GGCC'C
Cuts at: 2532 5172 5265 2532
Size: 2640 93 7345
Fragments arranged by size:
7345 2640 93
ApaLI G'TGCA_C
Cuts at: 7240 8135 9291 7240
Size: 895 1156 8027
Fragments arranged by size:
8027 1156 895
ApoI r'AATT_y
Cuts at: 1493 7387 7613 7765 9874 1493
Size: 5894 226 152 2109 1697
Fragments arranged by size:
5894 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8632 160
Size: 872 7600 1606
Fragments arranged by size:
7600 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10078
AvaI C'yCGr_G
Cuts at: 1870 1943 2055 3313 3821 4109 4675 5107 5619
Size: 73 112 1258 508 288 566 432 512
Cuts at: 5619 6911 6957 7403 1870
Size: 1292 46 446 4545
Fragments arranged by size:
4545 1292 1258 566 512 508 446 432
288 112 73 46
BamHI G'GATC_C
Cuts at: 7408 7775 7408
Size: 367 9711
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 3473 4179 4495 6336
Size: 325 217 627 516 1170 706 316 1841
Cuts at: 6336 6857 7047 7107 7399 8854 618
Size: 521 190 60 292 1455 1842
Fragments arranged by size:
1842 1841 1455 1170 706 627 521 516
325 316 292 217 190 60
BanII G_rGCy'C
Cuts at: 1391 2532 3239 3372 3708 3756 4110 4682 5172
Size: 1141 707 133 336 48 354 572 490
Cuts at: 5172 5265 5418 6288 6912 6984 7397 1391
Size: 93 153 870 624 72 413 4072
Fragments arranged by size:
4072 1141 870 707 624 572 490 413
354 336 153 133 93 72 48
BbeI G_GCGC'C
Cuts at: 6340 7111 6340
Size: 771 9307
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2656 2909 3649 6169 6482 928
Size: 971 757 253 740 2520 313 4524
Fragments arranged by size:
4524 2520 971 757 740 313 253
BbvCI CC'TCA_GC
Cuts at: 3038 5173 6609 3038
Size: 2135 1436 6507
Fragments arranged by size:
6507 2135 1436
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4469 4503 8269 8303 1903
Size: 34 2532 34 3766 34 3678
Fragments arranged by size:
3766 3678 2532 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2261 5978 7970 9180 2261
Size: 3717 1992 1210 3159
Fragments arranged by size:
3717 3159 1992 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 10078
BfrBI ATG'CAT
Cuts at: 4632 5796 7291 4632
Size: 1164 1495 7419
Fragments arranged by size:
7419 1495 1164
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3437 4970 8689
Size: 107 122 71 631 605 1765 1533 3719
Cuts at: 8689 136
Size: 1525
Fragments arranged by size:
3719 1765 1533 1525 631 605 122 107
71
BglII A'GATC_T
Cuts at: 5727 10073 5727
Size: 4346 5732
BlpI GC'TnA_GC
Cuts at: 3366 3873 5585 5660 6287 6452 7495 3366
Size: 507 1712 75 627 165 1043 5949
Fragments arranged by size:
5949 1712 1043 627 507 165 75
Bme1580I G_kGCm'C
Cuts at: 1790 2532 2792 3633 4500 5172 5214 5265 5649
Size: 742 260 841 867 672 42 51 384
Cuts at: 5649 7244 8139 9295 1790
Size: 1595 895 1156 2573
Fragments arranged by size:
2573 1595 1156 895 867 841 742 672
384 260 51 42
BmgBI CAC'GTC
Cuts at: 1318 4191 5164 5461 5581 6721 7074 1318
Size: 2873 973 297 120 1140 353 4322
Fragments arranged by size:
4322 2873 1140 973 353 297 120
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3895 7809 8767
Size: 416 53 83 399 24 2478 3914 958
Cuts at: 8767 442
Size: 1753
Fragments arranged by size:
3914 2478 1753 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10078
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2563 4516 5422 6949 2563
Size: 1953 906 1527 5692
Fragments arranged by size:
5692 1953 1527 906
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3219 3655 3932 3941 3986 4031 4054 4064
Size: 1980 436 277 9 45 45 23 10
Cuts at: 4064 4457 4486 4796 5759 5907 6418 6464 6530
Size: 393 29 310 963 148 511 46 66
Cuts at: 6530 6568 6650 7082 7333 8738 1239
Size: 38 82 432 251 1405 2579
Fragments arranged by size:
2579 1980 1405 963 511 436 432 393
310 277 251 148 82 66 46 45
45 38 29 23 10 9
Bpu10I CC'TnA_GC
Cuts at: 3029 3038 4445 4877 5173 6609 3029
Size: 9 1407 432 296 1436 6498
Fragments arranged by size:
6498 1436 1407 432 296 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2783 2956 3016 4214 7199 8203 9068 9366 2783
Size: 173 60 1198 2985 1004 865 298 3495
Fragments arranged by size:
3495 2985 1198 1004 865 298 173 60
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2633 2696 3017 3371 4536 5921 6506
Size: 617 1134 63 321 354 1165 1385 585
Cuts at: 6506 8741 882
Size: 2235 2219
Fragments arranged by size:
2235 2219 1385 1165 1134 617 585 354
321 63
BsaAI yAC'GTr
Cuts at: 493 1204 2536 6301 7869 493
Size: 711 1332 3765 1568 2702
Fragments arranged by size:
3765 2702 1568 1332 711
BsaBI GATnn'nnATC
Cuts at: 10072 10072
Size: 10078
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2141 3866 4692 4785
Size: 53 83 186 936 608 1725 826 93
Cuts at: 4785 5013 5333 6337 7108 7885 8267 8902 275
Size: 228 320 1004 771 777 382 635 1451
Fragments arranged by size:
1725 1451 1004 936 826 777 771 635
608 382 320 228 186 93 83 53
BsaWI w'CCGG_w
Cuts at: 2873 3414 3660 3807 4164 4792 5981 6009 8511
Size: 541 246 147 357 628 1189 28 2502
Cuts at: 8511 8909 9183 9330 9741 2873
Size: 398 274 147 411 3210
Fragments arranged by size:
3210 2502 1189 628 541 411 398 357
274 246 147 147 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3959 3989 6400 6430 6527
Size: 30 165 30 2556 30 2411 30 97
Cuts at: 6527 6557 1178
Size: 30 4699
Fragments arranged by size:
4699 2556 2411 165 97 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2255 2936 3338 3401 3659 4007 4109 4112
Size: 1069 681 402 63 258 348 102 3
Cuts at: 4112 4115 4118 4724 4742 4745 4748 4751 4754
Size: 3 3 606 18 3 3 3 3
Cuts at: 4754 4757 4760 4763 4766 4775 4778 4781 5216
Size: 3 3 3 3 9 3 3 435
Cuts at: 5216 5801 6395 6571 6583 6811 1186
Size: 585 594 176 12 228 4453
Fragments arranged by size:
4453 1069 681 606 594 585 435 402
348 258 228 176 102 63 18 12
9 3 3 3 3 3 3 3
3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2524 2753 3755 3897 5995 6141 6519 6984 7080
Size: 229 1002 142 2098 146 378 465 96
Cuts at: 7080 9281 9777 2524
Size: 2201 496 2825
Fragments arranged by size:
2825 2201 2098 1002 496 465 378 229
146 142 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 5178 6121 7037 1163
Size: 573 3442 943 916 4204
Fragments arranged by size:
4204 3442 943 916 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4483 4816 4834 5766 7032 7528
Size: 275 186 2482 333 18 932 1266 496
Cuts at: 7528 8289 9317 1540
Size: 761 1028 2301
Fragments arranged by size:
2482 2301 1266 1028 932 761 496 333
275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2517 3372 3651 4800 5586 6288 7164 7244 8139
Size: 855 279 1149 786 702 876 80 895
Cuts at: 8139 8224 9295 2517
Size: 85 1071 3300
Fragments arranged by size:
3300 1149 1071 895 876 855 786 702
279 85 80
BsmI GAATG_Cn'
Cuts at: 7589 7682 7589
Size: 93 9985
BsmBI CGTCTCn'nnnn_
Cuts at: 2132 3857 4197 4352 2132
Size: 1725 340 155 7858
Fragments arranged by size:
7858 1725 340 155
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2517 2532 2792 3239 3372 3633 3651
Size: 399 727 15 260 447 133 261 18
Cuts at: 3651 3708 3756 4110 4500 4682 4800 5172 5214
Size: 57 48 354 390 182 118 372 42
Cuts at: 5214 5265 5418 5586 5649 6288 6912 6984 7164
Size: 51 153 168 63 639 624 72 180
Cuts at: 7164 7244 7397 8139 8224 9295 1391
Size: 80 153 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 727 639 624 447 399
390 372 354 261 260 182 180 168
153 153 133 118 85 80 72 63
57 51 48 42 18 15
BspEI T'CCGG_A
Cuts at: 2873 3414 3660 4164 4792 5981 9741 2873
Size: 541 246 504 628 1189 3760 3210
Fragments arranged by size:
3760 3210 1189 628 541 504 246
BspHI T'CATG_A
Cuts at: 7967 9697 7967
Size: 1730 8348
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2209 4014 4212 4335 4893 5718 6498 7059
Size: 1365 1805 198 123 558 825 780 561
Cuts at: 7059 7433 844
Size: 374 3489
Fragments arranged by size:
3489 1805 1365 825 780 561 558 374
198 123
BsrBI CCG'CTC
Cuts at: 1394 1823 2764 3988 4135 5106 6601 7153 7965
Size: 429 941 1224 147 971 1495 552 812
Cuts at: 7965 1394
Size: 3507
Fragments arranged by size:
3507 1495 1224 971 941 812 552 429
147
BsrDI GCAATG_nn'
Cuts at: 65 1248 3079 8573 8747 65
Size: 1183 1831 5494 174 1396
Fragments arranged by size:
5494 1831 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2306 4535 4833 6145 6333 8722 8909
Size: 110 496 2229 298 1312 188 2389 187
Cuts at: 8909 1700
Size: 2869
Fragments arranged by size:
2869 2389 2229 1312 496 298 188 187
110
BsrGI T'GTAC_A
Cuts at: 96 7125 96
Size: 7029 3049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10078
BssSI C'ACGA_G
Cuts at: 3293 8138 9150 3293
Size: 4845 1012 4221
Fragments arranged by size:
4845 4221 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2521 5793 6490 7210 794
Size: 1727 3272 697 720 3662
Fragments arranged by size:
3662 3272 1727 720 697
BstBI TT'CG_AA
Cuts at: 8882 8882
Size: 10078
BstEII G'GTnAC_C
Cuts at: 1184 3130 3694 4531 5482 6076 1184
Size: 1946 564 837 951 594 5186
Fragments arranged by size:
5186 1946 951 837 594 564
BstXI CCAn_nnnn'nTGG
Cuts at: 2617 3167 3223 3418 4252 2617
Size: 550 56 195 834 8443
Fragments arranged by size:
8443 834 550 195 56
BstYI r'GATC_y
Cuts at: 1972 3063 5727 7014 7408 7775 8160 8177 9629
Size: 1091 2664 1287 394 367 385 17 1452
Cuts at: 9629 9715 9727 10073 1972
Size: 86 12 346 1977
Fragments arranged by size:
2664 1977 1452 1287 1091 394 385 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2190 5594 6869 2190
Size: 3404 1275 5399
Fragments arranged by size:
5399 3404 1275
BtgI C'CryG_G
Cuts at: 513 1065 2004 2427 2616 2715 2775 4389 5088
Size: 552 939 423 189 99 60 1614 699
Cuts at: 5088 5766 5779 6574 7146 7252 513
Size: 678 13 795 572 106 3339
Fragments arranged by size:
3339 1614 939 795 699 678 572 552
423 189 106 99 60 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5455 487
Size: 683 56 4229 5110
Fragments arranged by size:
5110 4229 683 56
BtsI GCAGTG_nn'
Cuts at: 2519 3649 5214 5481 5695 6241 7173 7594 8388
Size: 1130 1565 267 214 546 932 421 794
Cuts at: 8388 8408 2519
Size: 20 4189
Fragments arranged by size:
4189 1565 1130 932 794 546 421 267
214 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 10078
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2095 2130 529
Size: 35 1531 35 8477
Fragments arranged by size:
8477 1531 35 35
DraI TTT'AAA
Cuts at: 7382 7382
Size: 10078
DraIII CAC_nnn'GTG
Cuts at: 4915 4915
Size: 10078
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3728 5714 5935 7336 9085 809
Size: 507 2412 1986 221 1401 1749 1802
Fragments arranged by size:
2412 1986 1802 1749 1401 507 221
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2622 3438
Size: 54 1425 50 275 18 177 615 816
Cuts at: 3438 3453 4386 4813 4962 5539 5763 6660 6804
Size: 15 933 427 149 577 224 897 144
Cuts at: 6804 6893 7170 7323 7525 8414 10043 8
Size: 89 277 153 202 889 1629 43
Fragments arranged by size:
1629 1425 933 897 889 816 615 577
427 277 275 224 202 177 153 149
144 89 54 50 43 18 15
EagI C'GGCC_G
Cuts at: 1537 1812 4813 5763 7525 1537
Size: 275 3001 950 1762 4090
Fragments arranged by size:
4090 3001 1762 950 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2387 4623 4983 5118 5983 8008 1353
Size: 1034 2236 360 135 865 2025 3423
Fragments arranged by size:
3423 2236 2025 1034 865 360 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2030 2842 3415 4554 5006 5279
Size: 1064 138 441 812 573 1139 452 273
Cuts at: 5279 8665 9039 9185 387
Size: 3386 374 146 1280
Fragments arranged by size:
3386 1280 1139 1064 812 573 452 441
374 273 146 138
EcoICRI GAG'CTC
Cuts at: 3370 6286 3370
Size: 2916 7162
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2137 3140 3219 3362 3655 3932 3941
Size: 393 505 1003 79 143 293 277 9
Cuts at: 3941 3986 4031 4054 4064 4457 4486 4796 5441
Size: 45 45 23 10 393 29 310 645
Cuts at: 5441 5759 5907 6418 6464 6530 6538 6568 6650
Size: 318 148 511 46 66 8 30 82
Cuts at: 6650 7082 7333 8141 8738 9525 9793 1239
Size: 432 251 808 597 787 268 1524
Fragments arranged by size:
1524 1003 808 787 645 597 511 505
432 393 393 318 310 293 277 268
251 148 143 82 79 66 46 45
45 30 29 23 10 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 2801 2840 3524 4232 5573 5816 6461 6464 7265
Size: 39 684 708 1341 243 645 3 801
Cuts at: 7265 2801
Size: 5614
Fragments arranged by size:
5614 1341 801 708 684 645 243 39
3
EcoO109I rG'GnC_Cy
Cuts at: 2377 2528 2529 2542 2584 3091 3145 3203 3529
Size: 151 1 13 42 507 54 58 326
Cuts at: 3529 4201 4771 4819 4863 4993 5168 5169 5262
Size: 672 570 48 44 130 175 1 93
Cuts at: 5262 5438 5598 5687 5802 6402 6778 6873 2377
Size: 176 160 89 115 600 376 95 5582
Fragments arranged by size:
5582 672 600 570 507 376 326 176
175 160 151 130 115 95 93 89
58 54 48 44 42 13 1 1
EcoRI G'AATT_C
Cuts at: 7387 7765 7387
Size: 378 9700
FspI TGC'GCA
Cuts at: 8584 8584
Size: 10078
HaeII r_GCGC'y
Cuts at: 1678 2692 4154 6334 6340 6591 6991 7093 7111
Size: 1014 1462 2180 6 251 400 102 18
Cuts at: 7111 9225 1678
Size: 2114 2531
Fragments arranged by size:
2531 2180 2114 1462 1014 400 251 102
18 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2402 2434 2632
Size: 32 135 32 797 32 1 32 198
Cuts at: 2632 2664 4991 5023 6081 6113 6400 6432 6527
Size: 32 2327 32 1058 32 287 32 95
Cuts at: 6527 6554 6559 6586 6778 6810 8719 8751 8793
Size: 27 5 27 192 32 1909 32 42
Cuts at: 8793 8825 1373
Size: 32 2626
Fragments arranged by size:
2626 2327 1909 1058 797 287 198 192
135 95 42 32 32 32 32 32
32 32 32 32 32 32 27 27
5 1
HincII GTy'rAC
Cuts at: 669 1657 1938 2119 2937 3517 5188 7422 7668
Size: 988 281 181 818 580 1671 2234 246
Cuts at: 7668 669
Size: 3079
Fragments arranged by size:
3079 2234 1671 988 818 580 281 246
181
HindIII A'AGCT_T
Cuts at: 748 2149 748
Size: 1401 8677
HpaI GTT'AAC
Cuts at: 2119 7668 2119
Size: 5549 4529
KasI G'GCGC_C
Cuts at: 6336 7107 6336
Size: 771 9307
KpnI G_GTAC'C
Cuts at: 4183 7051 7403 4183
Size: 2868 352 6858
Fragments arranged by size:
6858 2868 352
MfeI C'AATT_G
Cuts at: 7677 7677
Size: 10078
MluI A'CGCG_T
Cuts at: 2205 9954 2205
Size: 7749 2329
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4713 9192 9376 1305
Size: 3408 4479 184 2007
Fragments arranged by size:
4479 3408 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3455 5541 6806 7172 10
Size: 54 1425 343 1623 2086 1265 366 2916
Fragments arranged by size:
2916 2086 1623 1425 1265 366 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2090 2324 2630 3416 3581 3785 3929
Size: 648 924 234 306 786 165 204 144
Cuts at: 3929 4250 4382 4631 5474 6359 8036 8395 8554
Size: 321 132 249 843 885 1677 359 159
Cuts at: 8554 518
Size: 2042
Fragments arranged by size:
2042 1677 924 885 843 786 648 359
321 306 249 234 204 165 159 144
132
MspA1I CmG'CkG
Cuts at: 1606 3069 3535 3872 4021 5877 6523 6652 7254
Size: 1463 466 337 149 1856 646 129 602
Cuts at: 7254 7494 8171 9319 9564 1606
Size: 240 677 1148 245 2120
Fragments arranged by size:
2120 1856 1463 1148 677 646 602 466
337 245 240 149 129
NaeI GCC'GGC
Cuts at: 2308 6147 6335 2308
Size: 3839 188 6051
Fragments arranged by size:
6051 3839 188
NarI GG'CG_CC
Cuts at: 6337 7108 6337
Size: 771 9307
NcoI C'CATG_G
Cuts at: 513 1065 2004 6574 513
Size: 552 939 4570 4017
Fragments arranged by size:
4570 4017 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10078
NgoMIV G'CCGG_C
Cuts at: 2306 6145 6333 2306
Size: 3839 188 6051
Fragments arranged by size:
6051 3839 188
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10078
NotI GC'GGCC_GC
Cuts at: 7525 7525
Size: 10078
NsiI A_TGCA'T
Cuts at: 4634 5798 7293 4634
Size: 1164 1495 7419
Fragments arranged by size:
7419 1495 1164
NspI r_CATG'y
Cuts at: 1132 1229 2258 2419 4632 5791 7126 7291 7436
Size: 97 1029 161 2213 1159 1335 165 145
Cuts at: 7436 8981 1132
Size: 1545 2229
Fragments arranged by size:
2229 2213 1545 1335 1159 1029 165 161
145 97
PasI CC'CwG_GG
Cuts at: 1348 2836 3160 6320 1348
Size: 1488 324 3160 5106
Fragments arranged by size:
5106 3160 1488 324
PciI A'CATG_T
Cuts at: 2415 7122 8977 2415
Size: 4707 1855 3516
Fragments arranged by size:
4707 3516 1855
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3964 1108
Size: 2856 7222
PfoI T'CCnGG_A
Cuts at: 2580 2949 3957 4398 5683 6348 6531 6774 7008
Size: 369 1008 441 1285 665 183 243 234
Cuts at: 7008 7059 2580
Size: 51 5599
Fragments arranged by size:
5599 1285 1008 665 441 369 243 234
183 51
PmeI GTTT'AAAC
Cuts at: 7382 7382
Size: 10078
PmlI CAC'GTG
Cuts at: 6301 6301
Size: 10078
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8147 8179 9660 9692 8147
Size: 32 1481 32 8533
Fragments arranged by size:
8533 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2377 2542 2584 3091 3145 3203 3529 4201 4771
Size: 165 42 507 54 58 326 672 570
Cuts at: 4771 4819 5687 5802 6778 6873 2377
Size: 48 868 115 976 95 5582
Fragments arranged by size:
5582 976 868 672 570 507 326 165
115 95 58 54 48 42
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10078
PsiI TTA'TAA
Cuts at: 7648 7648
Size: 10078
PspOMI G'GGCC_C
Cuts at: 2528 5168 5261 2528
Size: 2640 93 7345
Fragments arranged by size:
7345 2640 93
PstI C_TGCA'G
Cuts at: 830 2253 3007 4056 4492 5331 6465 7269 7430
Size: 1423 754 1049 436 839 1134 804 161
Cuts at: 7430 830
Size: 3478
Fragments arranged by size:
3478 1423 1134 1049 839 804 754 436
161
PvuI CG_AT'CG
Cuts at: 2001 7032 2001
Size: 5031 5047
PvuII CAG'CTG
Cuts at: 3872 3872
Size: 10078
SacI G_AGCT'C
Cuts at: 3372 6288 3372
Size: 2916 7162
SacII CC_GC'GG
Cuts at: 7255 7255
Size: 10078
SalI G'TCGA_C
Cuts at: 1936 7420 1936
Size: 5484 4594
SanDI GG'GwC_CC
Cuts at: 4819 5687 6778 4819
Size: 868 1091 8119
Fragments arranged by size:
8119 1091 868
SapI GCTCTTCn'nnn_
Cuts at: 5118 5118
Size: 10078
SbfI CC_TGCA'GG
Cuts at: 5331 6465 7430 5331
Size: 1134 965 7979
Fragments arranged by size:
7979 1134 965
ScaI AGT'ACT
Cuts at: 8326 10038 8326
Size: 1712 8366
SfcI C'TryA_G
Cuts at: 826 1045 2249 3003 4052 4488 5327 6461 7265
Size: 219 1204 754 1049 436 839 1134 804
Cuts at: 7265 7426 8561 9242 9433 826
Size: 161 1135 681 191 1471
Fragments arranged by size:
1471 1204 1135 1134 1049 839 804 754
681 436 219 191 161
SfiI GGCCn_nnn'nGGCC
Cuts at: 4970 4970
Size: 10078
SfoI GGC'GCC
Cuts at: 6338 7109 6338
Size: 771 9307
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10078
SgrAI Cr'CCGG_yG
Cuts at: 1700 6333 1700
Size: 4633 5445
SmaI CCC'GGG
Cuts at: 1872 2057 3315 6959 7405 1872
Size: 185 1258 3644 446 4545
Fragments arranged by size:
4545 3644 1258 446 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2798 2971 2995 4229 4675 7214
Size: 197 926 855 173 24 1234 446 2539
Cuts at: 7214 8182 9083 9345 820
Size: 968 901 262 1553
Fragments arranged by size:
2539 1553 1234 968 926 901 855 446
262 197 173 24
SnaBI TAC'GTA
Cuts at: 493 7869 493
Size: 7376 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10078
SphI G_CATG'C
Cuts at: 1132 2258 5791 7436 1132
Size: 1126 3533 1645 3774
Fragments arranged by size:
3774 3533 1645 1126
SrfI GCCC'GGGC
Cuts at: 6959 6959
Size: 10078
SspI AAT'ATT
Cuts at: 5 52 8002 5
Size: 47 7950 2081
Fragments arranged by size:
7950 2081 47
StuI AGG'CCT
Cuts at: 1517 5223 5402 1517
Size: 3706 179 6193
Fragments arranged by size:
6193 3706 179
StyI C'CwwG_G
Cuts at: 513 1065 2004 2133 2784 3477 3699 6159 6574
Size: 552 939 129 651 693 222 2460 415
Cuts at: 6574 7517 513
Size: 943 3074
Fragments arranged by size:
3074 2460 943 939 693 651 552 415
222 129
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2532 2696 3437 4368 4436 5945 6009
Size: 1255 952 164 741 931 68 1509 64
Cuts at: 6009 6155 7237 8105 8122 8275 8460 8905 325
Size: 146 1082 868 17 153 185 445 1498
Fragments arranged by size:
1509 1498 1255 1082 952 931 868 741
445 185 164 153 146 68 64 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5502 6705 7125
Size: 274 80 33 51 1443 3525 1203 420
Cuts at: 7125 7188 8324 10036 96
Size: 63 1136 1712 138
Fragments arranged by size:
3525 1712 1443 1203 1136 420 274 138
80 63 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2259 2291 4193 4225 6419 6451 10038 10070 2259
Size: 32 1902 32 2194 32 3587 32 2267
Fragments arranged by size:
3587 2267 2194 1902 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4784 5488 1316
Size: 3468 704 5906
Fragments arranged by size:
5906 3468 704
XbaI T'CTAG_A
Cuts at: 7414 7414
Size: 10078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3223 4006 4387 7093 7180 3223
Size: 783 381 2706 87 6121
Fragments arranged by size:
6121 2706 783 381 87
XhoI C'TCGA_G
Cuts at: 1943 4675 1943
Size: 2732 7346
XmaI C'CCGG_G
Cuts at: 1870 2055 3313 6957 7403 1870
Size: 185 1258 3644 446 4545
Fragments arranged by size:
4545 3644 1258 446 185
XmnI GAAnn'nnTTC
Cuts at: 4949 5127 8207 4949
Size: 178 3080 6820
Fragments arranged by size:
6820 3080 178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4786 7886 276
Size: 53 83 186 936 3252 3100 2468
Fragments arranged by size:
3252 3100 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BstZ17I EcoRV
FalI FseI FspAI NruI PacI PspXI PsrI RsrII
SexAI SwaI