Restriction map for
pFN21AB0047
(Circular) (Six-base) MAPSORT of: pFN21AB0047.seq Check: 8605 from: 1 to: 10726
pFN21AB0047 10726 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 6706 6706
Size: 10726
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8536 278
Size: 53 83 186 936 7000 2468
Fragments arranged by size:
7000 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7684 8069 1937
Size: 5747 385 4594
Fragments arranged by size:
5747 4594 385
Acc65I G'GTAC_C
Cuts at: 8047 8047
Size: 10726
AclI AA'CG_TT
Cuts at: 1563 6675 8853 9226 1563
Size: 5112 2178 373 3063
Fragments arranged by size:
5112 3063 2178 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 3072 4643 4830 5166 5450 5654 5925
Size: 707 733 1571 187 336 284 204 271
Cuts at: 5925 6053 6125 6311 6995 7508 7889 8789 10173
Size: 128 72 186 684 513 381 900 1384
Cuts at: 10173 10441 1632
Size: 268 1917
Fragments arranged by size:
1917 1571 1384 900 733 707 684 513
381 336 284 271 268 204 187 186
128 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10529
AflIII A'CryG_T
Cuts at: 1203 7778 9625 10602 1203
Size: 6575 1847 977 1327
Fragments arranged by size:
6575 1847 1327 977
AgeI A'CCGG_T
Cuts at: 9557 9557
Size: 10726
AhdI GACnn_n'nnGTC
Cuts at: 3378 4879 9455 3378
Size: 1501 4576 4649
Fragments arranged by size:
4649 4576 1501
AleI CACnn'nnGTG
Cuts at: 1166 4616 1166
Size: 3450 7276
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4554 1184
Size: 3370 7356
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4332 4364 6660 6692 4332
Size: 32 2296 32 8366
Fragments arranged by size:
8366 2296 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2549 3490 4796 4960 5254 5782 6319 6382 7465
Size: 941 1306 164 294 528 537 63 1083
Cuts at: 7465 8469 10041 2549
Size: 1004 1572 3234
Fragments arranged by size:
3234 1572 1306 1083 1004 941 537 528
294 164 63
ApaI G_GGCC'C
Cuts at: 6571 6706 6571
Size: 135 10591
ApaLI G'TGCA_C
Cuts at: 8783 9939 8783
Size: 1156 9570
ApoI r'AATT_y
Cuts at: 1493 2765 3426 4265 5077 6011 8035 8261 8413
Size: 1272 661 839 812 934 2024 226 152
Cuts at: 8413 10522 1493
Size: 2109 1697
Fragments arranged by size:
2109 2024 1697 1272 934 839 812 661
226 152
AseI AT'TA_AT
Cuts at: 160 1032 9280 160
Size: 872 8248 1606
Fragments arranged by size:
8248 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10726
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 3213 5066 6564 6572 6899 7014
Size: 73 81 1189 1853 1498 8 327 115
Cuts at: 7014 7508 8051 1870
Size: 494 543 4545
Fragments arranged by size:
4545 1853 1498 1189 543 494 327 115
81 73 8
BamHI G'GATC_C
Cuts at: 2029 4168 4218 6108 8056 8423 2029
Size: 2139 50 1890 1948 367 4332
Fragments arranged by size:
4332 2139 1948 1890 367 50
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 7926 8047 9502 618
Size: 325 217 627 6139 121 1455 1842
Fragments arranged by size:
6139 1842 1455 627 325 217 121
BanII G_rGCy'C
Cuts at: 1391 4803 4993 5340 5721 5889 6571 6653 6706
Size: 3412 190 347 381 168 682 82 53
Cuts at: 6706 6900 7204 7962 8045 1391
Size: 194 304 758 83 4072
Fragments arranged by size:
4072 3412 758 682 381 347 304 194
190 168 83 82 53
BbeI G_GCGC'C
Cuts at: 7930 7930
Size: 10726
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2561 2735 3938 4600 5478 6131 6362
Size: 971 662 174 1203 662 878 653 231
Cuts at: 6362 6645 6759 928
Size: 283 114 4895
Fragments arranged by size:
4895 1203 971 878 662 662 653 283
231 174 114
BbvCI CC'TCA_GC
Cuts at: 7880 7880
Size: 10726
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5278 5312 8917 8951 1903
Size: 34 3341 34 3605 34 3678
Fragments arranged by size:
3678 3605 3341 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6046 8618 9828 6046
Size: 2572 1210 6944
Fragments arranged by size:
6944 2572 1210
BclI T'GATC_A
Cuts at: 1548 3136 6879 1548
Size: 1588 3743 5395
Fragments arranged by size:
5395 3743 1588
BfrBI ATG'CAT
Cuts at: 2496 3588 2496
Size: 1092 9634
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4508 6587 6815
Size: 107 122 71 631 605 2836 2079 228
Cuts at: 6815 7556 7832 7920 9337 136
Size: 741 276 88 1417 1525
Fragments arranged by size:
2836 2079 1525 1417 741 631 605 276
228 122 107 88 71
BglII A'GATC_T
Cuts at: 10721 10721
Size: 10726
BlpI GC'TnA_GC
Cuts at: 8143 8143
Size: 10726
Bme1580I G_kGCm'C
Cuts at: 1790 6571 6706 8787 9943 1790
Size: 4781 135 2081 1156 2573
Fragments arranged by size:
4781 2573 2081 1156 135
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 10726
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6205 8011 8457
Size: 416 53 83 399 24 4788 1806 446
Cuts at: 8457 9415 442
Size: 958 1753
Fragments arranged by size:
4788 1806 1753 958 446 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 7418 1056
Size: 6362 4364
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3457 5149 5737 3457
Size: 1692 588 8446
Fragments arranged by size:
8446 1692 588
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2922 4434 4742 5582 5732 5804 6203 6341
Size: 1683 1512 308 840 150 72 399 138
Cuts at: 6341 6371 6605 6746 6761 6812 6932 7031 7340
Size: 30 234 141 15 51 120 99 309
Cuts at: 7340 7487 7574 7595 7775 7790 7976 9386 1239
Size: 147 87 21 180 15 186 1410 2579
Fragments arranged by size:
2579 1683 1512 1410 840 399 309 308
234 186 180 150 147 141 138 120
99 87 72 51 30 21 15 15
Bpu10I CC'TnA_GC
Cuts at: 5918 6018 6098 6308 7847 7880 5918
Size: 100 80 210 1539 33 8764
Fragments arranged by size:
8764 1539 210 100 80 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2067 5324 5548 5666 6056 7607 8851 9716 10014
Size: 3257 224 118 390 1551 1244 865 298
Cuts at: 10014 2067
Size: 2779
Fragments arranged by size:
3257 2779 1551 1244 865 390 298 224
118
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3011 3598 5905 6825 9389 882
Size: 617 1512 587 2307 920 2564 2219
Fragments arranged by size:
2564 2307 2219 1512 920 617 587
BsaAI yAC'GTr
Cuts at: 493 1204 5603 8517 493
Size: 711 4399 2914 2702
Fragments arranged by size:
4399 2914 2702 711
BsaBI GATnn'nnATC
Cuts at: 4223 10720 4223
Size: 6497 4229
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7294 7927 8533 8915
Size: 53 83 186 936 5761 633 606 382
Cuts at: 8915 9550 275
Size: 635 1451
Fragments arranged by size:
5761 1451 936 635 633 606 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 9159 9557 9831 9978 10389 9159
Size: 398 274 147 411 9496
Fragments arranged by size:
9496 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2160 2190 5792 5822 1178
Size: 30 165 30 757 30 3602 30 6082
Fragments arranged by size:
6082 3602 757 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4739 4994 5066 5378 5381 5663 5729 6089
Size: 3553 255 72 312 3 282 66 360
Cuts at: 6089 6140 6170 6173 6176 6179 6338 6611 6758
Size: 51 30 3 3 3 159 273 147
Cuts at: 6758 7337 7340 7352 7592 1186
Size: 579 3 12 240 4320
Fragments arranged by size:
4320 3553 579 360 312 282 273 255
240 159 147 72 66 51 30 12
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2549 5589 6735 9929 10425 2549
Size: 3040 1146 3194 496 2850
Fragments arranged by size:
3194 3040 2850 1146 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 7919 1163
Size: 573 6183 3970
Fragments arranged by size:
6183 3970 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3247 5292 7369 8176 8937 9965
Size: 275 186 1246 2045 2077 807 761 1028
Cuts at: 9965 1540
Size: 2301
Fragments arranged by size:
2301 2077 2045 1246 1028 807 761 275
186
BsiHKAI G_wGCw'C
Cuts at: 4803 5340 5721 5853 5889 6653 6900 7962 8787
Size: 537 381 132 36 764 247 1062 825
Cuts at: 8787 8872 9943 4803
Size: 85 1071 5586
Fragments arranged by size:
5586 1071 1062 825 764 537 381 247
132 85 36
BsmI GAATG_Cn'
Cuts at: 3653 8237 8330 3653
Size: 4584 93 6049
Fragments arranged by size:
6049 4584 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5279 7997 5279
Size: 2718 8008
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4803 4993 5340 5721 5853 5889 6571
Size: 399 3013 190 347 381 132 36 682
Cuts at: 6571 6653 6706 6900 7204 7962 8045 8787 8872
Size: 82 53 194 304 758 83 742 85
Cuts at: 8872 9943 1391
Size: 1071 2174
Fragments arranged by size:
3013 2174 1071 758 742 682 399 381
347 304 194 190 132 85 83 82
53 36
BspEI T'CCGG_A
Cuts at: 10389 10389
Size: 10726
BspHI T'CATG_A
Cuts at: 2446 3949 6645 8615 10345 2446
Size: 1503 2696 1970 1730 2827
Fragments arranged by size:
2827 2696 1970 1730 1503
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3616 5304 5821 5866 6706 6843 7657 8081
Size: 2772 1688 517 45 840 137 814 424
Cuts at: 8081 844
Size: 3489
Fragments arranged by size:
3489 2772 1688 840 814 517 424 137
45
BsrBI CCG'CTC
Cuts at: 1394 1823 3325 4735 6508 7471 8613 1394
Size: 429 1502 1410 1773 963 1142 3507
Fragments arranged by size:
3507 1773 1502 1410 1142 963 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2217 3036 5118 9221 9395 65
Size: 1183 969 819 2082 4103 174 1396
Fragments arranged by size:
4103 2082 1396 1183 969 819 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7875 7923 9370 9557 1700
Size: 110 6065 48 1447 187 2869
Fragments arranged by size:
6065 2869 1447 187 110 48
BsrGI T'GTAC_A
Cuts at: 96 2427 4377 96
Size: 2331 1950 6445
Fragments arranged by size:
6445 2331 1950
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10726
BssSI C'ACGA_G
Cuts at: 2841 4145 7089 8786 9798 2841
Size: 1304 2944 1697 1012 3769
Fragments arranged by size:
3769 2944 1697 1304 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2689 3290 4835 5002 794
Size: 1895 601 1545 167 6518
Fragments arranged by size:
6518 1895 1545 601 167
BstBI TT'CG_AA
Cuts at: 9530 9530
Size: 10726
BstEII G'GTnAC_C
Cuts at: 1184 4493 1184
Size: 3309 7417
BstXI CCAn_nnnn'nTGG
Cuts at: 3188 4090 5983 3188
Size: 902 1893 7931
Fragments arranged by size:
7931 1893 902
BstYI r'GATC_y
Cuts at: 1972 2029 3574 4168 4218 4284 6108 8056 8423
Size: 57 1545 594 50 66 1824 1948 367
Cuts at: 8423 8808 8825 10277 10363 10375 10721 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1948 1824 1545 1452 594 385 367
346 86 66 57 50 17 12
Bsu36I CC'TnA_GG
Cuts at: 2637 2637
Size: 10726
BtgI C'CryG_G
Cuts at: 513 1065 2004 2131 3021 5580 6768 513
Size: 552 939 127 890 2559 1188 4471
Fragments arranged by size:
4471 2559 1188 939 890 552 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3332 487
Size: 683 56 2106 7881
Fragments arranged by size:
7881 2106 683 56
BtsI GCAGTG_nn'
Cuts at: 4601 8242 9036 9056 4601
Size: 3641 794 20 6271
Fragments arranged by size:
6271 3641 794 20
ClaI AT'CG_AT
Cuts at: 1554 3561 1554
Size: 2007 8719
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10691
DraI TTT'AAA
Cuts at: 4449 8030 4449
Size: 3581 7145
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2060 5876 9733 809
Size: 507 744 3816 3857 1802
Fragments arranged by size:
3857 3816 1802 744 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3244 3843 4500
Size: 54 1425 50 275 18 1414 599 657
Cuts at: 4500 5172 5352 5385 7921 8173 9062 10691 8
Size: 672 180 33 2536 252 889 1629 43
Fragments arranged by size:
2536 1629 1425 1414 889 672 657 599
275 252 180 54 50 43 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3244 8173 1537
Size: 275 1432 4929 4090
Fragments arranged by size:
4929 4090 1432 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2369 3051 4145 4668 4752 4812 4854 5043
Size: 1016 682 1094 523 84 60 42 189
Cuts at: 5043 5142 6117 6468 6473 6498 6855 7139 7329
Size: 99 975 351 5 25 357 284 190
Cuts at: 7329 7436 7912 8656 1353
Size: 107 476 744 3423
Fragments arranged by size:
3423 1094 1016 975 744 682 523 476
357 351 284 190 189 107 99 84
60 42 25 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5948 6920 7253 9313 9687 9833
Size: 1064 138 4359 972 333 2060 374 146
Cuts at: 9833 387
Size: 1280
Fragments arranged by size:
4359 2060 1280 1064 972 374 333 146
138
EcoICRI GAG'CTC
Cuts at: 4801 5338 5719 5887 6651 6898 7960 4801
Size: 537 381 168 764 247 1062 7567
Fragments arranged by size:
7567 1062 764 537 381 247 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2922 3072 4434 4643 4742 4830
Size: 393 707 583 150 1362 209 99 88
Cuts at: 4830 5166 5450 5582 5654 5732 5804 5925 6053
Size: 336 284 132 72 78 72 121 128
Cuts at: 6053 6125 6203 6311 6341 6371 6605 6746 6761
Size: 72 78 108 30 30 234 141 15
Cuts at: 6761 6812 6932 6995 7031 7340 7487 7508 7574
Size: 51 120 63 36 309 147 21 66
Cuts at: 7574 7595 7775 7790 7889 7976 8789 9386 10173
Size: 21 180 15 99 87 813 597 787
Cuts at: 10173 10441 1239
Size: 268 1524
Fragments arranged by size:
1524 1362 813 787 707 597 583 393
336 309 284 268 234 209 180 150
147 141 132 128 121 120 108 99
99 88 87 78 78 72 72 72
66 63 51 36 30 30 21 21
15 15
EcoNI CCTnn'n_nnAGG
Cuts at: 5828 6374 5828
Size: 546 10180
EcoO109I rG'GnC_Cy
Cuts at: 4957 5779 5821 6304 6568 6703 6732 6979 7843
Size: 822 42 483 264 135 29 247 864
Cuts at: 7843 4957
Size: 7840
Fragments arranged by size:
7840 864 822 483 264 247 135 42
29
EcoRI G'AATT_C
Cuts at: 4265 8035 8413 4265
Size: 3770 378 6578
Fragments arranged by size:
6578 3770 378
EcoRV GAT'ATC
Cuts at: 4462 4462
Size: 10726
FspI TGC'GCA
Cuts at: 3168 9232 3168
Size: 6064 4662
HaeII r_GCGC'y
Cuts at: 1678 6319 6418 6938 7260 7434 7930 9873 1678
Size: 4641 99 520 322 174 496 1943 2531
Fragments arranged by size:
4641 2531 1943 520 496 322 174 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2504 2536 2735 2767 3050
Size: 32 135 32 932 32 199 32 283
Cuts at: 3050 3082 3364 3396 4081 4113 4269 4276 4301
Size: 32 282 32 685 32 156 7 25
Cuts at: 4301 4308 4865 4897 5465 5497 5957 5989 7099
Size: 7 557 32 568 32 460 32 1110
Cuts at: 7099 7131 9367 9399 9441 9473 1373
Size: 32 2236 32 42 32 2626
Fragments arranged by size:
2626 2236 1110 932 685 568 557 460
283 282 199 156 135 42 32 32
32 32 32 32 32 32 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 2218 6799 7685 7999 8070 8316
Size: 988 281 280 4581 886 314 71 246
Cuts at: 8316 669
Size: 3079
Fragments arranged by size:
4581 3079 988 886 314 281 280 246
71
HindIII A'AGCT_T
Cuts at: 748 2298 3141 6284 6782 748
Size: 1550 843 3143 498 4692
Fragments arranged by size:
4692 3143 1550 843 498
HpaI GTT'AAC
Cuts at: 8316 8316
Size: 10726
KasI G'GCGC_C
Cuts at: 7926 7926
Size: 10726
KpnI G_GTAC'C
Cuts at: 8051 8051
Size: 10726
MfeI C'AATT_G
Cuts at: 6255 7169 8325 6255
Size: 914 1156 8656
Fragments arranged by size:
8656 1156 914
MluI A'CGCG_T
Cuts at: 7778 10602 7778
Size: 2824 7902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6086 6453 7989 9840 10024 1305
Size: 4781 367 1536 1851 184 2007
Fragments arranged by size:
4781 2007 1851 1536 367 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3845 4502 5174 5354 5387
Size: 54 1425 343 2013 657 672 180 33
Cuts at: 5387 10
Size: 5349
Fragments arranged by size:
5349 2013 1425 672 657 343 180 54
33
MslI CAynn'nnrTG
Cuts at: 518 1166 2466 3995 4616 8684 9043 9202 518
Size: 648 1300 1529 621 4068 359 159 2042
Fragments arranged by size:
4068 2042 1529 1300 648 621 359 159
MspA1I CmG'CkG
Cuts at: 1606 3490 4558 5008 5140 5350 5731 6118 6463
Size: 1884 1068 450 132 210 381 387 345
Cuts at: 6463 6739 6931 7318 7573 7753 7810 7933 8142
Size: 276 192 387 255 180 57 123 209
Cuts at: 8142 8819 9967 10212 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1884 1148 1068 677 450 387 387
381 345 276 255 245 210 209 192
180 132 123 57
NaeI GCC'GGC
Cuts at: 7925 7925
Size: 10726
NarI GG'CG_CC
Cuts at: 7927 7927
Size: 10726
NcoI C'CATG_G
Cuts at: 513 1065 2004 2131 3021 513
Size: 552 939 127 890 8218
Fragments arranged by size:
8218 939 890 552 127
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10726
NgoMIV G'CCGG_C
Cuts at: 7923 7923
Size: 10726
NheI G'CTAG_C
Cuts at: 1052 7414 1052
Size: 6362 4364
NotI GC'GGCC_GC
Cuts at: 8173 8173
Size: 10726
NsiI A_TGCA'T
Cuts at: 2498 3590 2498
Size: 1092 9634
NspI r_CATG'y
Cuts at: 1132 1229 2289 2500 4045 6805 8084 9629 1132
Size: 97 1060 211 1545 2760 1279 1545 2229
Fragments arranged by size:
2760 2229 1545 1545 1279 1060 211 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10726
PciI A'CATG_T
Cuts at: 9625 9625
Size: 10726
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6319 7465 1108
Size: 5211 1146 4369
Fragments arranged by size:
5211 4369 1146
PfoI T'CCnGG_A
Cuts at: 2986 3012 3405 4209 4260 5679 5880 2986
Size: 26 393 804 51 1419 201 7832
Fragments arranged by size:
7832 1419 804 393 201 51 26
PmeI GTTT'AAAC
Cuts at: 8030 8030
Size: 10726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4401 4433 8795 8827 10308 10340 4401
Size: 32 4362 32 1481 32 4787
Fragments arranged by size:
4787 4362 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4957 5821 6732 6979 4957
Size: 864 911 247 8704
Fragments arranged by size:
8704 911 864 247
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10726
PsiI TTA'TAA
Cuts at: 2378 8296 2378
Size: 5918 4808
PspOMI G'GGCC_C
Cuts at: 6567 6702 6567
Size: 135 10591
PspXI vC'TCGA_Gb
Cuts at: 2024 6572 6899 7508 2024
Size: 4548 327 609 5242
Fragments arranged by size:
5242 4548 609 327
PstI C_TGCA'G
Cuts at: 830 3486 3582 5007 5301 5322 5349 5376 5829
Size: 2656 96 1425 294 21 27 27 453
Cuts at: 5829 5955 6207 6375 7503 8078 830
Size: 126 252 168 1128 575 3478
Fragments arranged by size:
3478 2656 1425 1128 575 453 294 252
168 126 96 27 27 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10726
PvuII CAG'CTG
Cuts at: 3490 5008 5140 5350 5731 6118 6463 6739 7318
Size: 1518 132 210 381 387 345 276 579
Cuts at: 7318 7573 7753 7933 3490
Size: 255 180 180 6283
Fragments arranged by size:
6283 1518 579 387 381 345 276 255
210 180 180 132
SacI G_AGCT'C
Cuts at: 4803 5340 5721 5889 6653 6900 7962 4803
Size: 537 381 168 764 247 1062 7567
Fragments arranged by size:
7567 1062 764 537 381 247 168
SalI G'TCGA_C
Cuts at: 1936 7683 8068 1936
Size: 5747 385 4594
Fragments arranged by size:
5747 4594 385
SapI GCTCTTCn'nnn_
Cuts at: 4668 6473 6498 4668
Size: 1805 25 8896
Fragments arranged by size:
8896 1805 25
SbfI CC_TGCA'GG
Cuts at: 5301 8078 5301
Size: 2777 7949
ScaI AGT'ACT
Cuts at: 8974 10686 8974
Size: 1712 9014
SfcI C'TryA_G
Cuts at: 826 1045 2953 3095 3482 3578 5003 5297 5318
Size: 219 1908 142 387 96 1425 294 21
Cuts at: 5318 5345 5372 5825 5951 6047 6203 6371 6942
Size: 27 27 453 126 96 156 168 571
Cuts at: 6942 7388 7499 8074 9209 9890 10081 826
Size: 446 111 575 1135 681 191 1471
Fragments arranged by size:
1908 1471 1425 1135 681 575 571 453
446 387 294 219 191 168 156 142
126 111 96 96 27 27 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 4508 4508
Size: 10726
SfoI GGC'GCC
Cuts at: 7928 7928
Size: 10726
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10726
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10726
SmaI CCC'GGG
Cuts at: 1872 6566 8053 1872
Size: 4694 1487 4545
Fragments arranged by size:
4694 4545 1487
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2024 2082 5066 5339 5527 5645
Size: 197 926 81 58 2984 273 188 118
Cuts at: 5645 6035 6572 6899 7508 7586 8830 9731 9993
Size: 390 537 327 609 78 1244 901 262
Cuts at: 9993 820
Size: 1553
Fragments arranged by size:
2984 1553 1244 926 901 609 537 390
327 273 262 197 188 118 81 78
58
SnaBI TAC'GTA
Cuts at: 493 5603 8517 493
Size: 5110 2914 2702
Fragments arranged by size:
5110 2914 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10726
SphI G_CATG'C
Cuts at: 1132 2500 8084 1132
Size: 1368 5584 3774
Fragments arranged by size:
5584 3774 1368
SrfI GCCC'GGGC
Cuts at: 6566 6566
Size: 10726
SspI AAT'ATT
Cuts at: 5 52 2416 2632 3109 3910 8650 5
Size: 47 2364 216 477 801 4740 2081
Fragments arranged by size:
4740 2364 2081 801 477 216 47
StuI AGG'CCT
Cuts at: 1517 4334 4511 1517
Size: 2817 177 7732
Fragments arranged by size:
7732 2817 177
StyI C'CwwG_G
Cuts at: 513 1065 2004 2131 2259 3021 5895 6591 7599
Size: 552 939 127 128 762 2874 696 1008
Cuts at: 7599 8165 513
Size: 566 3074
Fragments arranged by size:
3074 2874 1008 939 762 696 566 552
128 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5306 8753 8770 8923 9108 9553 325
Size: 1255 3726 3447 17 153 185 445 1498
Fragments arranged by size:
3726 3447 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 3625 4377
Size: 274 80 33 51 1443 450 1198 752
Cuts at: 4377 8972 10684 96
Size: 4595 1712 138
Fragments arranged by size:
4595 1712 1443 1198 752 450 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4164 4196 5792 5824 10686 10718 4164
Size: 32 1596 32 4862 32 4172
Fragments arranged by size:
4862 4172 1596 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10726
XbaI T'CTAG_A
Cuts at: 5761 8062 5761
Size: 2301 8425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2897 6178 6661 6787 7390 7549 2897
Size: 3281 483 126 603 159 6074
Fragments arranged by size:
6074 3281 603 483 159 126
XhoI C'TCGA_G
Cuts at: 1943 2024 5066 6572 6899 7508 1943
Size: 81 3042 1506 327 609 5161
Fragments arranged by size:
5161 3042 1506 609 327 81
XmaI C'CCGG_G
Cuts at: 1870 6564 8051 1870
Size: 4694 1487 4545
Fragments arranged by size:
4694 4545 1487
XmnI GAAnn'nnTTC
Cuts at: 3060 3109 4656 4863 7164 8855 3060
Size: 49 1547 207 2301 1691 4931
Fragments arranged by size:
4931 2301 1691 1547 207 49
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8534 276
Size: 53 83 186 936 7000 2468
Fragments arranged by size:
7000 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BsiWI BstZ17I DraIII FalI
FseI FspAI NruI PacI PmlI PsrI RsrII SacII
SanDI SexAI SwaI