Restriction map for
pFN21AB0044
(Circular) (Six-base) MAPSORT of: pFN21AB0044.seq Check: 3716 from: 1 to: 8479
pFN21AB0044 8479 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6289 278
Size: 53 83 186 936 4753 2468
Fragments arranged by size:
4753 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5822 1937
Size: 3885 4594
Acc65I G'GTAC_C
Cuts at: 3641 5800 3641
Size: 2159 6320
AclI AA'CG_TT
Cuts at: 1563 6606 6979 1563
Size: 5043 373 3063
Fragments arranged by size:
5043 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 1998 2481 2801 3068 4430 6542 7926 8194
Size: 366 483 320 267 1362 2112 1384 268
Cuts at: 8194 1632
Size: 1917
Fragments arranged by size:
2112 1917 1384 1362 483 366 320 268
267
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8282
AflIII A'CryG_T
Cuts at: 1203 3883 7378 8355 1203
Size: 2680 3495 977 1327
Fragments arranged by size:
3495 2680 1327 977
AgeI A'CCGG_T
Cuts at: 7310 7310
Size: 8479
AhdI GACnn_n'nnGTC
Cuts at: 7208 7208
Size: 8479
AleI CACnn'nnGTG
Cuts at: 1166 4219 1166
Size: 3053 5426
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2849 3671 3861 4677 1184
Size: 1665 822 190 816 4986
Fragments arranged by size:
4986 1665 822 816 190
AlwNI CAG_nnn'CTG
Cuts at: 4342 4472 6222 7794 4342
Size: 130 1750 1572 5027
Fragments arranged by size:
5027 1750 1572 130
ApaLI G'TGCA_C
Cuts at: 6536 7692 6536
Size: 1156 7323
ApoI r'AATT_y
Cuts at: 1493 2465 2522 2543 4752 5030 5087 5152 5648
Size: 972 57 21 2209 278 57 65 496
Cuts at: 5648 5788 6014 6166 8275 1493
Size: 140 226 152 2109 1697
Fragments arranged by size:
2209 2109 1697 972 496 278 226 152
140 65 57 57 21
AseI AT'TA_AT
Cuts at: 160 1032 4421 7033 160
Size: 872 3389 2612 1606
Fragments arranged by size:
3389 2612 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8479
AvaI C'yCGr_G
Cuts at: 1870 1943 5804 1870
Size: 73 3861 4545
Fragments arranged by size:
4545 3861 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4973 5006 4973
Size: 33 8446
BamHI G'GATC_C
Cuts at: 5809 6176 5809
Size: 367 8112
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3641 5800 7255 618
Size: 325 217 627 1854 2159 1455 1842
Fragments arranged by size:
2159 1854 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3479 5592 5798 1391
Size: 2088 2113 206 4072
Fragments arranged by size:
4072 2113 2088 206
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3243 4032 4172 5499 928
Size: 971 1344 789 140 1327 3908
Fragments arranged by size:
3908 1344 1327 971 789 140
BbvCI CC'TCA_GC
Cuts at: 3615 3615
Size: 8479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5543 5577 6670 6704 1903
Size: 34 3606 34 1093 34 3678
Fragments arranged by size:
3678 3606 1093 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5001 6371 7581 5001
Size: 1370 1210 5899
Fragments arranged by size:
5899 1370 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8479
BfrBI ATG'CAT
Cuts at: 4654 4654
Size: 8479
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7090 136
Size: 107 122 71 631 605 5418 1525
Fragments arranged by size:
5418 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5463 8474 5463
Size: 3011 5468
BlpI GC'TnA_GC
Cuts at: 5586 5706 5896 5586
Size: 120 190 8169
Fragments arranged by size:
8169 190 120
Bme1580I G_kGCm'C
Cuts at: 1790 6540 7696 1790
Size: 4750 1156 2573
Fragments arranged by size:
4750 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4874 1318
Size: 3556 4923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2291 3508 5175
Size: 416 53 83 399 24 874 1217 1667
Cuts at: 5175 6210 7168 442
Size: 1035 958 1753
Fragments arranged by size:
1753 1667 1217 1035 958 874 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8479
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2148 3318 3612 4344 5565 7139 1239
Size: 909 1170 294 732 1221 1574 2579
Fragments arranged by size:
2579 1574 1221 1170 909 732 294
Bpu10I CC'TnA_GC
Cuts at: 3615 5711 3615
Size: 2096 6383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3465 6604 7469 7767 3465
Size: 3139 865 298 4177
Fragments arranged by size:
4177 3139 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5388 7142 882
Size: 617 3889 1754 2219
Fragments arranged by size:
3889 2219 1754 617
BsaAI yAC'GTr
Cuts at: 493 1204 3886 6270 493
Size: 711 2682 2384 2702
Fragments arranged by size:
2702 2682 2384 711
BsaBI GATnn'nnATC
Cuts at: 5462 8473 5462
Size: 3011 5468
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6286 6668 7303 275
Size: 53 83 186 936 4753 382 635 1451
Fragments arranged by size:
4753 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6912 7310 7584 7731 8142 6912
Size: 398 274 147 411 7249
Fragments arranged by size:
7249 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8254
Fragments arranged by size:
8254 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2214 3379 4481 4547 1186
Size: 1028 1165 1102 66 5118
Fragments arranged by size:
5118 1165 1102 1028 66
BseYI C'CCAG_C
Cuts at: 7682 8178 7682
Size: 496 7983
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7906
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5929 6690 7718 1540
Size: 275 186 3928 761 1028 2301
Fragments arranged by size:
3928 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3479 3577 6540 6625 7696 3479
Size: 98 2963 85 1071 4262
Fragments arranged by size:
4262 2963 1071 98 85
BsmI GAATG_Cn'
Cuts at: 2440 5772 5990 6083 2440
Size: 3332 218 93 4836
Fragments arranged by size:
4836 3332 218 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3479 3577 5592 5798 6540 6625 7696
Size: 399 1689 98 2015 206 742 85 1071
Cuts at: 7696 1391
Size: 2174
Fragments arranged by size:
2174 2015 1689 1071 742 399 206 98
85
BspEI T'CCGG_A
Cuts at: 8142 8142
Size: 8479
BspHI T'CATG_A
Cuts at: 2691 3022 6368 8098 2691
Size: 331 3346 1730 3072
Fragments arranged by size:
3346 3072 1730 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5834 844
Size: 4990 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6366 1394
Size: 429 4543 3507
Fragments arranged by size:
4543 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2301 3558 6974 7148 65
Size: 1183 1053 1257 3416 174 1396
Fragments arranged by size:
3416 1396 1257 1183 1053 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2127 3644 7123 7310 1700
Size: 110 317 1517 3479 187 2869
Fragments arranged by size:
3479 2869 1517 317 187 110
BsrGI T'GTAC_A
Cuts at: 96 3596 96
Size: 3500 4979
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8479
BssSI C'ACGA_G
Cuts at: 4914 6539 7551 4914
Size: 1625 1012 5842
Fragments arranged by size:
5842 1625 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4216 794
Size: 3422 5057
BstBI TT'CG_AA
Cuts at: 2422 7283 2422
Size: 4861 3618
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8479
BstXI CCAn_nnnn'nTGG
Cuts at: 4853 4853
Size: 8479
BstYI r'GATC_y
Cuts at: 1972 4135 5463 5809 6176 6561 6578 8030 8116
Size: 2163 1328 346 367 385 17 1452 86
Cuts at: 8116 8128 8474 1972
Size: 12 346 1977
Fragments arranged by size:
2163 1977 1452 1328 385 367 346 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2209 2304 5388 5661 513
Size: 552 1144 95 3084 273 3331
Fragments arranged by size:
3331 3084 1144 552 273 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2332 3260 487
Size: 683 56 1106 928 5706
Fragments arranged by size:
5706 1106 928 683 56
BtsI GCAGTG_nn'
Cuts at: 3943 4214 4226 4343 5995 6789 6809 3943
Size: 271 12 117 1652 794 20 5613
Fragments arranged by size:
5613 1652 794 271 117 20 12
ClaI AT'CG_AT
Cuts at: 1554 4006 1554
Size: 2452 6027
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8444
DraI TTT'AAA
Cuts at: 4963 5783 4963
Size: 820 7659
DraIII CAC_nnn'GTG
Cuts at: 3262 3262
Size: 8479
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2632 2645 7486 809
Size: 507 1316 13 4841 1802
Fragments arranged by size:
4841 1802 1316 507 13
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2125 5926 6815
Size: 54 1425 50 275 18 295 3801 889
Cuts at: 6815 8444 8
Size: 1629 43
Fragments arranged by size:
3801 1629 1425 889 295 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5926 1537
Size: 275 4114 4090
Fragments arranged by size:
4114 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2168 2209 2780 3024 6409 1353
Size: 815 41 571 244 3385 3423
Fragments arranged by size:
3423 3385 815 571 244 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7066 7440 7586 387
Size: 1064 138 5477 374 146 1280
Fragments arranged by size:
5477 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3477 3477
Size: 8479
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1998 2148 2481 2801 3068 3318 3612
Size: 393 366 150 333 320 267 250 294
Cuts at: 3612 4344 4430 5565 6542 7139 7926 8194 1239
Size: 732 86 1135 977 597 787 268 1524
Fragments arranged by size:
1524 1135 977 787 732 597 393 366
333 320 294 268 267 250 150 86
EcoNI CCTnn'n_nnAGG
Cuts at: 3537 3537
Size: 8479
EcoO109I rG'GnC_Cy
Cuts at: 4441 4820 4441
Size: 379 8100
EcoRI G'AATT_C
Cuts at: 5788 6166 5788
Size: 378 8101
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3242 3242
Size: 8479
FseI GG_CCGG'CC
Cuts at: 2131 2131
Size: 8479
FspI TGC'GCA
Cuts at: 6985 6985
Size: 8479
HaeII r_GCGC'y
Cuts at: 1678 7626 1678
Size: 5948 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4823 4855 5171 5203 5311
Size: 32 135 32 3251 32 316 32 108
Cuts at: 5311 5343 7120 7152 7194 7226 1373
Size: 32 1777 32 42 32 2626
Fragments arranged by size:
3251 2626 1777 316 135 108 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3967 4098 4648 5823 6069 669
Size: 988 281 2029 131 550 1175 246 3079
Fragments arranged by size:
3079 2029 1175 988 550 281 246 131
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8479
HpaI GTT'AAC
Cuts at: 4098 6069 4098
Size: 1971 6508
KpnI G_GTAC'C
Cuts at: 3645 5804 3645
Size: 2159 6320
MfeI C'AATT_G
Cuts at: 6078 6078
Size: 8479
MluI A'CGCG_T
Cuts at: 8355 8355
Size: 8479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2784 7593 7777 1305
Size: 1479 4809 184 2007
Fragments arranged by size:
4809 2007 1479 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 6657
Fragments arranged by size:
6657 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2064 3213 3401 3441 3843 4219 4851
Size: 648 898 1149 188 40 402 376 632
Cuts at: 4851 4941 5534 6437 6796 6955 518
Size: 90 593 903 359 159 2042
Fragments arranged by size:
2042 1149 903 898 648 632 593 402
376 359 188 159 90 40
MspA1I CmG'CkG
Cuts at: 1606 3296 3985 4661 5895 6572 7720 7965 1606
Size: 1690 689 676 1234 677 1148 245 2120
Fragments arranged by size:
2120 1690 1234 1148 689 677 676 245
NaeI GCC'GGC
Cuts at: 2129 2129
Size: 8479
NcoI C'CATG_G
Cuts at: 513 1065 5388 5661 513
Size: 552 4323 273 3331
Fragments arranged by size:
4323 3331 552 273
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8479
NgoMIV G'CCGG_C
Cuts at: 2127 2127
Size: 8479
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8479
NotI GC'GGCC_GC
Cuts at: 5926 5926
Size: 8479
NsiI A_TGCA'T
Cuts at: 4656 4656
Size: 8479
NspI r_CATG'y
Cuts at: 1132 1229 3446 4654 4658 5558 5837 7382 1132
Size: 97 2217 1208 4 900 279 1545 2229
Fragments arranged by size:
2229 2217 1545 1208 900 279 97 4
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8479
PciI A'CATG_T
Cuts at: 7378 7378
Size: 8479
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4472 4546 1108
Size: 3364 74 5041
Fragments arranged by size:
5041 3364 74
PmeI GTTT'AAAC
Cuts at: 5783 5783
Size: 8479
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6548 6580 8061 8093 6548
Size: 32 1481 32 6934
Fragments arranged by size:
6934 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4820 4820
Size: 8479
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8479
PsiI TTA'TAA
Cuts at: 6049 6049
Size: 8479
PstI C_TGCA'G
Cuts at: 830 3069 3757 3914 4221 4666 5737 5831 830
Size: 2239 688 157 307 445 1071 94 3478
Fragments arranged by size:
3478 2239 1071 688 445 307 157 94
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8479
PvuII CAG'CTG
Cuts at: 3296 3985 4661 3296
Size: 689 676 7114
Fragments arranged by size:
7114 689 676
SacI G_AGCT'C
Cuts at: 3479 3479
Size: 8479
SalI G'TCGA_C
Cuts at: 1936 5821 1936
Size: 3885 4594
SapI GCTCTTCn'nnn_
Cuts at: 2168 2209 2168
Size: 41 8438
SbfI CC_TGCA'GG
Cuts at: 5831 5831
Size: 8479
ScaI AGT'ACT
Cuts at: 5022 6727 8439 5022
Size: 1705 1712 5062
Fragments arranged by size:
5062 1712 1705
SfcI C'TryA_G
Cuts at: 826 1045 3065 3753 3814 3910 3922 4153 4217
Size: 219 2020 688 61 96 12 231 64
Cuts at: 4217 4662 5146 5733 5827 6962 7643 7834 826
Size: 445 484 587 94 1135 681 191 1471
Fragments arranged by size:
2020 1471 1135 688 681 587 484 445
231 219 191 96 94 64 61 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8479
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8479
SmaI CCC'GGG
Cuts at: 1872 5806 1872
Size: 3934 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3480 6583 7484 7746 820
Size: 197 926 1537 3103 901 262 1553
Fragments arranged by size:
3103 1553 1537 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6270 493
Size: 5777 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8479
SphI G_CATG'C
Cuts at: 1132 4658 5558 5837 1132
Size: 3526 900 279 3774
Fragments arranged by size:
3774 3526 900 279
SspI AAT'ATT
Cuts at: 5 52 6403 5
Size: 47 6351 2081
Fragments arranged by size:
6351 2081 47
StuI AGG'CCT
Cuts at: 1517 4586 1517
Size: 3069 5410
StyI C'CwwG_G
Cuts at: 513 1065 5388 5661 5918 513
Size: 552 4323 273 257 3074
Fragments arranged by size:
4323 3074 552 273 257
SwaI ATTT'AAAT
Cuts at: 4963 4963
Size: 8479
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6506 6523 6676 6861 7306 325
Size: 1255 4926 17 153 185 445 1498
Fragments arranged by size:
4926 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3264 3596 5020
Size: 274 80 33 51 1443 1287 332 1424
Cuts at: 5020 5365 6725 8437 96
Size: 345 1360 1712 138
Fragments arranged by size:
1712 1443 1424 1360 1287 345 332 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8439 8471 8439
Size: 32 8447
Tth111I GACn'n_nGTC
Cuts at: 1316 2568 1316
Size: 1252 7227
XbaI T'CTAG_A
Cuts at: 3901 5815 3901
Size: 1914 6565
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2066 2800 2066
Size: 734 7745
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8479
XmaI C'CCGG_G
Cuts at: 1870 5804 1870
Size: 3934 4545
XmnI GAAnn'nnTTC
Cuts at: 3183 3534 4806 6608 3183
Size: 351 1272 1802 5054
Fragments arranged by size:
5054 1802 1272 351
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6287 276
Size: 53 83 186 936 4753 2468
Fragments arranged by size:
4753 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BplI
BsiWI BsmBI BstZ17I Bsu36I EcoRV FspAI KasI NarI
NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI