Restriction map for
pFN21AB0039
(Circular) (Six-base) MAPSORT of: pFN21AB0039.seq Check: 9451 from: 1 to: 6241
pFN21AB0039 6241 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3312 3312
Size: 6241
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4051 278
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3584 1937
Size: 1647 4594
Acc65I G'GTAC_C
Cuts at: 2921 3562 2921
Size: 641 5600
AclI AA'CG_TT
Cuts at: 1563 3514 4368 4741 1563
Size: 1951 854 373 3063
Fragments arranged by size:
3063 1951 854 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2771 4304 5688 5956 1632
Size: 1139 1533 1384 268 1917
Fragments arranged by size:
1917 1533 1384 1139 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6044
AflIII A'CryG_T
Cuts at: 1203 5140 6117 1203
Size: 3937 977 1327
Fragments arranged by size:
3937 1327 977
AgeI A'CCGG_T
Cuts at: 5072 5072
Size: 6241
AhdI GACnn_n'nnGTC
Cuts at: 4970 4970
Size: 6241
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6241
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3207 1184
Size: 2023 4218
AlwNI CAG_nnn'CTG
Cuts at: 2717 3331 3984 5556 2717
Size: 614 653 1572 3402
Fragments arranged by size:
3402 1572 653 614
ApaLI G'TGCA_C
Cuts at: 2437 4298 5454 2437
Size: 1861 1156 3224
Fragments arranged by size:
3224 1861 1156
ApoI r'AATT_y
Cuts at: 1493 2062 2633 2869 3550 3776 3928 6037 1493
Size: 569 571 236 681 226 152 2109 1697
Fragments arranged by size:
2109 1697 681 571 569 236 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4795 160
Size: 872 3763 1606
Fragments arranged by size:
3763 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
AvaI C'yCGr_G
Cuts at: 1870 1943 3172 3566 1870
Size: 73 1229 394 4545
Fragments arranged by size:
4545 1229 394 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3160 3193 3160
Size: 33 6208
BamHI G'GATC_C
Cuts at: 3571 3938 3571
Size: 367 5874
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2921 3134 3562 5017
Size: 325 217 627 306 828 213 428 1455
Cuts at: 5017 618
Size: 1842
Fragments arranged by size:
1842 1455 828 627 428 325 306 217
213
BanII G_rGCy'C
Cuts at: 1391 3034 3560 1391
Size: 1643 526 4072
Fragments arranged by size:
4072 1643 526
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2406 928
Size: 971 507 4763
Fragments arranged by size:
4763 971 507
BbvCI CC'TCA_GC
Cuts at: 2954 2954
Size: 6241
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4432 4466 1903
Size: 34 2495 34 3678
Fragments arranged by size:
3678 2495 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3188 4133 5343 3188
Size: 945 1210 4086
Fragments arranged by size:
4086 1210 945
BclI T'GATC_A
Cuts at: 1548 3322 1548
Size: 1774 4467
BfrBI ATG'CAT
Cuts at: 2350 2350
Size: 6241
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4852 136
Size: 107 122 71 631 605 3180 1525
Fragments arranged by size:
3180 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2119 6236 2119
Size: 4117 2124
BlpI GC'TnA_GC
Cuts at: 3658 3658
Size: 6241
Bme1580I G_kGCm'C
Cuts at: 1790 2441 4302 5458 1790
Size: 651 1861 1156 2573
Fragments arranged by size:
2573 1861 1156 651
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6241
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2752 3972 4930
Size: 416 53 83 399 24 1335 1220 958
Cuts at: 4930 442
Size: 1753
Fragments arranged by size:
1753 1335 1220 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6241
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2629 2629
Size: 6241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2669 3069 4901 1239
Size: 1430 400 1832 2579
Fragments arranged by size:
2579 1832 1430 400
Bpu10I CC'TnA_GC
Cuts at: 2519 2822 2954 3401 2519
Size: 303 132 447 5359
Fragments arranged by size:
5359 447 303 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4366 5231 5529 4366
Size: 865 298 5078
Fragments arranged by size:
5078 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4904 882
Size: 617 3405 2219
Fragments arranged by size:
3405 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4032 493
Size: 711 2828 2702
Fragments arranged by size:
2828 2702 711
BsaBI GATnn'nnATC
Cuts at: 6235 6235
Size: 6241
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4048 4430 5065 275
Size: 53 83 186 936 2515 382 635 1451
Fragments arranged by size:
2515 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4674 5072 5346 5493 5904 4674
Size: 398 274 147 411 5011
Fragments arranged by size:
5011 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6016
Fragments arranged by size:
6016 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2791 1186
Size: 1605 4636
BseYI C'CCAG_C
Cuts at: 2676 5444 5940 2676
Size: 2768 496 2977
Fragments arranged by size:
2977 2768 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2862 2919 3524 1163
Size: 573 1126 57 605 3880
Fragments arranged by size:
3880 1126 605 573 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3691 4452 5480 1540
Size: 275 186 1690 761 1028 2301
Fragments arranged by size:
2301 1690 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2441 2964 3146 4302 4387 5458 2441
Size: 523 182 1156 85 1071 3224
Fragments arranged by size:
3224 1156 1071 523 182 85
BsmI GAATG_Cn'
Cuts at: 2320 2495 2857 3752 3845 2320
Size: 175 362 895 93 4716
Fragments arranged by size:
4716 895 362 175 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2080 2080
Size: 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2441 2964 3034 3146 3560 4302 4387
Size: 399 651 523 70 112 414 742 85
Cuts at: 4387 5458 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 651 523 414 399 112
85 70
BspEI T'CCGG_A
Cuts at: 5904 5904
Size: 6241
BspHI T'CATG_A
Cuts at: 4130 5860 4130
Size: 1730 4511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2380 3312 3339 3596 844
Size: 1536 932 27 257 3489
Fragments arranged by size:
3489 1536 932 257 27
BsrBI CCG'CTC
Cuts at: 1394 1823 4128 1394
Size: 429 2305 3507
Fragments arranged by size:
3507 2305 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2050 2589 2862 4736 4910 65
Size: 1183 802 539 273 1874 174 1396
Fragments arranged by size:
1874 1396 1183 802 539 273 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4885 5072 1700
Size: 110 3075 187 2869
Fragments arranged by size:
3075 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3213 96
Size: 3117 3124
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6241
BssSI C'ACGA_G
Cuts at: 4301 5313 4301
Size: 1012 5229
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2265 2695 2885 2897 794
Size: 1471 430 190 12 4138
Fragments arranged by size:
4138 1471 430 190 12
BstBI TT'CG_AA
Cuts at: 2193 3518 5045 2193
Size: 1325 1527 3389
Fragments arranged by size:
3389 1527 1325
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6241
BstXI CCAn_nnnn'nTGG
Cuts at: 2163 3317 2163
Size: 1154 5087
BstYI r'GATC_y
Cuts at: 1972 2119 3571 3938 4323 4340 5792 5878 5890
Size: 147 1452 367 385 17 1452 86 12
Cuts at: 5890 6236 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1452 385 367 346 147 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2228 2228
Size: 6241
BtgI C'CryG_G
Cuts at: 513 1065 2004 2559 2601 2622 3147 513
Size: 552 939 555 42 21 525 3607
Fragments arranged by size:
3607 939 555 552 525 42 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5502
Fragments arranged by size:
5502 683 56
BtsI GCAGTG_nn'
Cuts at: 3124 3757 4551 4571 3124
Size: 633 794 20 4794
Fragments arranged by size:
4794 794 633 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6206
DraI TTT'AAA
Cuts at: 3099 3545 3099
Size: 446 5795
DraIII CAC_nnn'GTG
Cuts at: 2918 2918
Size: 6241
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2456 5248 809
Size: 507 1140 2792 1802
Fragments arranged by size:
2792 1802 1140 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3688 4577 6206
Size: 54 1425 50 275 18 1858 889 1629
Cuts at: 6206 8
Size: 43
Fragments arranged by size:
1858 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3688 1537
Size: 275 1876 4090
Fragments arranged by size:
4090 1876 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2195 2325 2473 2660 4171 1353
Size: 842 130 148 187 1511 3423
Fragments arranged by size:
3423 1511 842 187 148 130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4828 5202 5348 387
Size: 1064 138 3239 374 146 1280
Fragments arranged by size:
3239 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2669 2771 3069 4304 4901 5688 5956
Size: 393 1037 102 298 1235 597 787 268
Cuts at: 5956 1239
Size: 1524
Fragments arranged by size:
1524 1235 1037 787 597 393 298 268
102
EcoNI CCTnn'n_nnAGG
Cuts at: 3161 3308 3161
Size: 147 6094
EcoO109I rG'GnC_Cy
Cuts at: 2160 2507 2976 3154 3328 2160
Size: 347 469 178 174 5073
Fragments arranged by size:
5073 469 347 178 174
EcoRI G'AATT_C
Cuts at: 2062 3550 3928 2062
Size: 1488 378 4375
Fragments arranged by size:
4375 1488 378
FspI TGC'GCA
Cuts at: 4747 4747
Size: 6241
HaeII r_GCGC'y
Cuts at: 1678 5388 1678
Size: 3710 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2032 2039 2064 2071 4882
Size: 32 135 32 460 7 25 7 2811
Cuts at: 4882 4914 4956 4988 1373
Size: 32 42 32 2626
Fragments arranged by size:
2811 2626 460 135 42 32 32 32
32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3010 3585 3831 669
Size: 988 281 1072 575 246 3079
Fragments arranged by size:
3079 1072 988 575 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6241
HpaI GTT'AAC
Cuts at: 3831 3831
Size: 6241
KpnI G_GTAC'C
Cuts at: 2925 3566 2925
Size: 641 5600
MfeI C'AATT_G
Cuts at: 3840 3840
Size: 6241
MluI A'CGCG_T
Cuts at: 6117 6117
Size: 6241
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2001 5355 5539 1305
Size: 696 3354 184 2007
Fragments arranged by size:
3354 2007 696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4419
Fragments arranged by size:
4419 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2719 4199 4558 4717 518
Size: 648 1553 1480 359 159 2042
Fragments arranged by size:
2042 1553 1480 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3657 4334 5482 5727 1606
Size: 2051 677 1148 245 2120
Fragments arranged by size:
2120 2051 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2559 2601 2622 3147 513
Size: 552 939 555 42 21 525 3607
Fragments arranged by size:
3607 939 555 552 525 42 21
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6241
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6241
NotI GC'GGCC_GC
Cuts at: 3688 3688
Size: 6241
NsiI A_TGCA'T
Cuts at: 2352 2352
Size: 6241
NspI r_CATG'y
Cuts at: 1132 1229 3599 5144 1132
Size: 97 2370 1545 2229
Fragments arranged by size:
2370 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2342 1348
Size: 994 5247
PciI A'CATG_T
Cuts at: 5140 5140
Size: 6241
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2839 1108
Size: 1731 4510
PmeI GTTT'AAAC
Cuts at: 3545 3545
Size: 6241
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4310 4342 5823 5855 4310
Size: 32 1481 32 4696
Fragments arranged by size:
4696 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2160 2507 2976 3328 2160
Size: 347 469 352 5073
Fragments arranged by size:
5073 469 352 347
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6241
PsiI TTA'TAA
Cuts at: 3811 3811
Size: 6241
PstI C_TGCA'G
Cuts at: 830 2893 3039 3339 3593 830
Size: 2063 146 300 254 3478
Fragments arranged by size:
3478 2063 300 254 146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6241
SalI G'TCGA_C
Cuts at: 1936 3583 1936
Size: 1647 4594
SapI GCTCTTCn'nnn_
Cuts at: 2325 2325
Size: 6241
SbfI CC_TGCA'GG
Cuts at: 3593 3593
Size: 6241
ScaI AGT'ACT
Cuts at: 4489 6201 4489
Size: 1712 4529
SexAI A'CCwGG_T
Cuts at: 2162 2162
Size: 6241
SfcI C'TryA_G
Cuts at: 826 1045 2889 3035 3335 3589 4724 5405 5596
Size: 219 1844 146 300 254 1135 681 191
Cuts at: 5596 826
Size: 1471
Fragments arranged by size:
1844 1471 1135 681 300 254 219 191
146
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6241
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6241
SmaI CCC'GGG
Cuts at: 1872 3568 1872
Size: 1696 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4345 5246 5508 820
Size: 197 926 2402 901 262 1553
Fragments arranged by size:
2402 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4032 493
Size: 3539 2702
SpeI A'CTAG_T
Cuts at: 152 2966 152
Size: 2814 3427
SphI G_CATG'C
Cuts at: 1132 3599 1132
Size: 2467 3774
SspI AAT'ATT
Cuts at: 5 52 4165 5
Size: 47 4113 2081
Fragments arranged by size:
4113 2081 47
StuI AGG'CCT
Cuts at: 1517 3167 1517
Size: 1650 4591
StyI C'CwwG_G
Cuts at: 513 1065 2004 2156 2559 2601 2622 3147 3372
Size: 552 939 152 403 42 21 525 225
Cuts at: 3372 3680 513
Size: 308 3074
Fragments arranged by size:
3074 939 552 525 403 308 225 152
42 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2121 2635 2850 4268 4285 4438 4623
Size: 1255 541 514 215 1418 17 153 185
Cuts at: 4623 5068 325
Size: 445 1498
Fragments arranged by size:
1498 1418 1255 541 514 445 215 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3213 4487 6199
Size: 274 80 33 51 1443 1236 1274 1712
Cuts at: 6199 96
Size: 138
Fragments arranged by size:
1712 1443 1274 1236 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6201 6233 6201
Size: 32 6209
Tth111I GACn'n_nGTC
Cuts at: 1316 2164 1316
Size: 848 5393
XbaI T'CTAG_A
Cuts at: 3577 3577
Size: 6241
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6241
XmaI C'CCGG_G
Cuts at: 1870 3566 1870
Size: 1696 4545
XmnI GAAnn'nnTTC
Cuts at: 4370 4370
Size: 6241
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4049 276
Size: 53 83 186 936 2515 2468
Fragments arranged by size:
2515 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BsiWI BstZ17I
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI
SwaI XcmI