Restriction map for
pFN21AB0023
(Circular) (Six-base) MAPSORT of: pFN21AB0023.seq Check: 8369 from: 1 to: 11050
pFN21AB0023 11050 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4772 6643 4772
Size: 1871 9179
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7392 8860 278
Size: 53 83 186 936 5856 1468 2468
Fragments arranged by size:
5856 2468 1468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2056 8393 1937
Size: 119 6337 4594
Fragments arranged by size:
6337 4594 119
Acc65I G'GTAC_C
Cuts at: 8371 8371
Size: 11050
AclI AA'CG_TT
Cuts at: 1563 9177 9550 1563
Size: 7614 373 3063
Fragments arranged by size:
7614 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2163 2520 2681 2912 2930 3137 3596 3704
Size: 531 357 161 231 18 207 459 108
Cuts at: 3704 3860 4241 4371 5294 5640 5910 6344 6740
Size: 156 381 130 923 346 270 434 396
Cuts at: 6740 6975 7016 7826 8137 9113 10497 10765 1632
Size: 235 41 810 311 976 1384 268 1917
Fragments arranged by size:
1917 1384 976 923 810 531 459 434
396 381 357 346 311 270 268 235
231 207 161 156 130 108 41 18
AfeI AGC'GCT
Cuts at: 4902 4902
Size: 11050
AflII C'TTAA_G
Cuts at: 820 1017 3790 820
Size: 197 2773 8080
Fragments arranged by size:
8080 2773 197
AflIII A'CryG_T
Cuts at: 1203 2059 9949 10926 1203
Size: 856 7890 977 1327
Fragments arranged by size:
7890 1327 977 856
AgeI A'CCGG_T
Cuts at: 9881 9881
Size: 11050
AhdI GACnn_n'nnGTC
Cuts at: 9779 9779
Size: 11050
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 11050
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2222 3762 6147 1184
Size: 1038 1540 2385 6087
Fragments arranged by size:
6087 2385 1540 1038
AlwNI CAG_nnn'CTG
Cuts at: 2349 2908 3394 3757 4549 5728 5797 5947 6070
Size: 559 486 363 792 1179 69 150 123
Cuts at: 6070 6112 6280 6397 6718 7291 8793 10365 2349
Size: 42 168 117 321 573 1502 1572 3034
Fragments arranged by size:
3034 1572 1502 1179 792 573 559 486
363 321 168 150 123 117 69 42
ApaI G_GGCC'C
Cuts at: 3487 4690 6211 7690 7997 3487
Size: 1203 1521 1479 307 6540
Fragments arranged by size:
6540 1521 1479 1203 307
ApaLI G'TGCA_C
Cuts at: 9107 10263 9107
Size: 1156 9894
ApoI r'AATT_y
Cuts at: 1493 2370 2399 7100 8359 8585 8737 10846 1493
Size: 877 29 4701 1259 226 152 2109 1697
Fragments arranged by size:
4701 2109 1697 1259 877 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 9604 160
Size: 872 8572 1606
Fragments arranged by size:
8572 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11050
AvaI C'yCGr_G
Cuts at: 1870 1943 2022 3894 4806 4850 5453 5472 5646
Size: 73 79 1872 912 44 603 19 174
Cuts at: 5646 6047 6420 6510 7402 8052 8325 8375 1870
Size: 401 373 90 892 650 273 50 4545
Fragments arranged by size:
4545 1872 912 892 650 603 401 373
273 174 90 79 73 50 44 19
BamHI G'GATC_C
Cuts at: 8380 8747 8380
Size: 367 10683
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4487 6545 7247 7850 8136
Size: 325 217 627 2700 2058 702 603 286
Cuts at: 8136 8315 8371 9826 618
Size: 179 56 1455 1842
Fragments arranged by size:
2700 2058 1842 1455 702 627 603 325
286 217 179 56
BanII G_rGCy'C
Cuts at: 1391 2333 2508 2829 3102 3425 3487 3525 4599
Size: 942 175 321 273 323 62 38 1074
Cuts at: 4599 4635 4690 5326 6211 7140 7690 7986 7997
Size: 36 55 636 885 929 550 296 11
Cuts at: 7997 8369 1391
Size: 372 4072
Fragments arranged by size:
4072 1074 942 929 885 636 550 372
323 321 296 273 175 62 55 38
36 11
BbeI G_GCGC'C
Cuts at: 4491 8140 4491
Size: 3649 7401
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2918 7004 7384 7814 928
Size: 971 1019 4086 380 430 4164
Fragments arranged by size:
4164 4086 1019 971 430 380
BbvCI CC'TCA_GC
Cuts at: 3437 6480 3437
Size: 3043 8007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2198 2232 2729 2763 7766 7800 9241
Size: 34 261 34 497 34 5003 34 1441
Cuts at: 9241 9275 1903
Size: 34 3678
Fragments arranged by size:
5003 3678 1441 497 261 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8942 10152 8942
Size: 1210 9840
BclI T'GATC_A
Cuts at: 1548 3405 1548
Size: 1857 9193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3473 3503 4340
Size: 107 122 71 631 605 1801 30 837
Cuts at: 4340 4646 4855 5474 5642 6125 9661 136
Size: 306 209 619 168 483 3536 1525
Fragments arranged by size:
3536 1801 1525 837 631 619 605 483
306 209 168 122 107 71 30
BglII A'GATC_T
Cuts at: 11045 11045
Size: 11050
BlpI GC'TnA_GC
Cuts at: 3101 3360 3671 3947 4629 5460 5478 5961 6093
Size: 259 311 276 682 831 18 483 132
Cuts at: 6093 6273 6314 6825 7146 7616 8467 3101
Size: 180 41 511 321 470 851 5684
Fragments arranged by size:
5684 851 831 682 511 483 470 321
311 276 259 180 132 41 18
Bme1580I G_kGCm'C
Cuts at: 1790 3487 3907 4690 5053 5544 6211 7690 7721
Size: 1697 420 783 363 491 667 1479 31
Cuts at: 7721 7796 7853 7940 7997 9111 10267 1790
Size: 75 57 87 57 1114 1156 2573
Fragments arranged by size:
2573 1697 1479 1156 1114 783 667 491
420 363 87 75 57 57 31
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11050
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2359 6793 8781
Size: 416 53 83 399 24 942 4434 1988
Cuts at: 8781 9739 442
Size: 958 1753
Fragments arranged by size:
4434 1988 1753 958 942 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2717 3611 7545 1056
Size: 1661 894 3934 4561
Fragments arranged by size:
4561 3934 1661 894
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3484 5485 5968 3484
Size: 2001 483 8566
Fragments arranged by size:
8566 2001 483
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2589 2723 2729 2843 3110 3341 3479
Size: 839 511 134 6 114 267 231 138
Cuts at: 3479 3842 3851 3875 3953 4088 4100 4325 4583
Size: 363 9 24 78 135 12 225 258
Cuts at: 4583 4643 5090 5240 5264 5390 5516 5750 5819
Size: 60 447 150 24 126 126 234 69
Cuts at: 5819 6179 6539 6584 6755 6938 7073 7281 7334
Size: 360 360 45 171 183 135 208 53
Cuts at: 7334 7382 7677 7844 7899 8094 9710 1239
Size: 48 295 167 55 195 1616 2579
Fragments arranged by size:
2579 1616 839 511 447 363 360 360
295 267 258 234 231 225 208 195
183 171 167 150 138 135 135 134
126 126 114 78 69 60 55 53
48 45 24 24 12 9 6
Bpu10I CC'TnA_GC
Cuts at: 3437 5317 5557 6480 6735 7737 7841 3437
Size: 1880 240 923 255 1002 104 6646
Fragments arranged by size:
6646 1880 1002 923 255 240 104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2858 3797 5114 7183 9175 10040 10338 2858
Size: 939 1317 2069 1992 865 298 3570
Fragments arranged by size:
3570 2069 1992 1317 939 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2961 4223 4757 6461 7083 7875 7886
Size: 617 1462 1262 534 1704 622 792 11
Cuts at: 7886 7978 8049 9713 882
Size: 92 71 1664 2219
Fragments arranged by size:
2219 1704 1664 1462 1262 792 622 617
534 92 71 11
BsaAI yAC'GTr
Cuts at: 493 1204 2062 6455 8841 493
Size: 711 858 4393 2386 2702
Fragments arranged by size:
4393 2702 2386 858 711
BsaBI GATnn'nnATC
Cuts at: 11044 11044
Size: 11050
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4488 4876 7389 8137
Size: 53 83 186 936 2955 388 2513 748
Cuts at: 8137 8857 9239 9874 275
Size: 720 382 635 1451
Fragments arranged by size:
2955 2513 1451 936 748 720 635 388
382 186 83 53
BsaWI w'CCGG_w
Cuts at: 9483 9881 10155 10302 10713 9483
Size: 398 274 147 411 9820
Fragments arranged by size:
9820 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3603 3633 3686 3716 4466
Size: 30 165 30 2200 30 53 30 750
Cuts at: 4466 4496 5690 5720 1178
Size: 30 1194 30 6508
Fragments arranged by size:
6508 2200 1194 750 165 53 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2560 2723 2855 2972 3056 3788 4178 4487
Size: 1374 163 132 117 84 732 390 309
Cuts at: 4487 5009 5534 5711 5714 5816 5894 5915 5987
Size: 522 525 177 3 102 78 21 72
Cuts at: 5987 6167 6365 6581 7208 7549 7880 1186
Size: 180 198 216 627 341 331 4356
Fragments arranged by size:
4356 1374 732 627 525 522 390 341
331 309 216 198 180 177 163 132
117 102 84 78 72 21 3
BseYI C'CCAG_C
Cuts at: 3084 3189 3229 3603 3750 3927 4289 4389 5977
Size: 105 40 374 147 177 362 100 1588
Cuts at: 5977 6179 6210 6364 6762 6777 6967 6989 7152
Size: 202 31 154 398 15 190 22 163
Cuts at: 7152 7236 7284 10253 10749 3084
Size: 84 48 2969 496 3385
Fragments arranged by size:
3385 2969 1588 496 398 374 362 202
190 177 163 154 147 105 100 84
48 40 31 22 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2016 2218 2238 5906 6506 6725 1163
Size: 573 280 202 20 3668 600 219 5488
Fragments arranged by size:
5488 3668 600 573 280 219 202 20
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 8500 9261 10289 1540
Size: 275 186 6499 761 1028 2301
Fragments arranged by size:
6499 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2333 2508 3048 3102 3425 3525 4599 4635 4657
Size: 175 540 54 323 100 1074 36 22
Cuts at: 4657 5307 5494 6054 6506 6831 8084 9111 9196
Size: 650 187 560 452 325 1253 1027 85
Cuts at: 9196 10267 2333
Size: 1071 3116
Fragments arranged by size:
3116 1253 1074 1071 1027 650 560 540
452 325 323 187 175 100 85 54
36 22
BsiWI C'GTAC_G
Cuts at: 6451 6451
Size: 11050
BsmI GAATG_Cn'
Cuts at: 2246 8561 8654 2246
Size: 6315 93 4642
Fragments arranged by size:
6315 4642 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2647 7380 2647
Size: 4733 6317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2333 2508 2829 3048 3102 3425 3487
Size: 399 543 175 321 219 54 323 62
Cuts at: 3487 3525 3907 4599 4635 4657 4690 5053 5307
Size: 38 382 692 36 22 33 363 254
Cuts at: 5307 5326 5494 5544 6054 6211 6506 6831 7140
Size: 19 168 50 510 157 295 325 309
Cuts at: 7140 7690 7721 7796 7853 7940 7986 7997 8084
Size: 550 31 75 57 87 46 11 87
Cuts at: 8084 8369 9111 9196 10267 1391
Size: 285 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 692 550 543 510 399
382 363 325 323 321 309 295 285
254 219 175 168 157 87 87 85
75 62 57 54 50 46 38 36
33 31 22 19 11
BspEI T'CCGG_A
Cuts at: 10713 10713
Size: 11050
BspHI T'CATG_A
Cuts at: 8939 10669 8939
Size: 1730 9320
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3423 4654 4772 5943 6643 7028 7820 8405
Size: 2579 1231 118 1171 700 385 792 585
Cuts at: 8405 844
Size: 3489
Fragments arranged by size:
3489 2579 1231 1171 792 700 585 385
118
BsrBI CCG'CTC
Cuts at: 1394 1823 3364 3799 4450 4918 5041 5464 5482
Size: 429 1541 435 651 468 123 423 18
Cuts at: 5482 6373 7419 7424 8937 1394
Size: 891 1046 5 1513 3507
Fragments arranged by size:
3507 1541 1513 1046 891 651 468 435
429 423 123 18 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 4945 5414 7761 8271 9545 9719 65
Size: 1183 3697 469 2347 510 1274 174 1396
Fragments arranged by size:
3697 2347 1396 1274 1183 510 469 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2170 4608 5652 6387 8133 9694 9881
Size: 110 360 2438 1044 735 1746 1561 187
Cuts at: 9881 1700
Size: 2869
Fragments arranged by size:
2869 2438 1746 1561 1044 735 360 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11050
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11050
BssSI C'ACGA_G
Cuts at: 9110 10122 9110
Size: 1012 10038
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6340 7064 7118 794
Size: 5546 724 54 4726
Fragments arranged by size:
5546 4726 724 54
BstBI TT'CG_AA
Cuts at: 3375 9854 3375
Size: 6479 4571
BstEII G'GTnAC_C
Cuts at: 1184 4141 1184
Size: 2957 8093
BstXI CCAn_nnnn'nTGG
Cuts at: 2821 3479 7997 2821
Size: 658 4518 5874
Fragments arranged by size:
5874 4518 658
BstYI r'GATC_y
Cuts at: 1972 7180 8380 8747 9132 9149 10601 10687 10699
Size: 5208 1200 367 385 17 1452 86 12
Cuts at: 10699 11045 1972
Size: 346 1977
Fragments arranged by size:
5208 1977 1452 1200 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2134 2616 4380 513
Size: 552 1069 482 1764 7183
Fragments arranged by size:
7183 1764 1069 552 482
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10311
Fragments arranged by size:
10311 683 56
BtsI GCAGTG_nn'
Cuts at: 2903 3762 5375 6504 6603 8254 8298 8566 9360
Size: 859 1613 1129 99 1651 44 268 794
Cuts at: 9360 9380 2903
Size: 20 4573
Fragments arranged by size:
4573 1651 1613 1129 859 794 268 99
44 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11050
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5194 5229 529
Size: 35 4630 35 6350
Fragments arranged by size:
6350 4630 35 35
DraI TTT'AAA
Cuts at: 7125 8354 7125
Size: 1229 9821
DraIII CAC_nnn'GTG
Cuts at: 3661 3661
Size: 11050
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 10057 809
Size: 507 8741 1802
Fragments arranged by size:
8741 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2811 3759 4647
Size: 54 1425 50 275 18 981 948 888
Cuts at: 4647 5079 5202 5772 6385 8497 9386 11015 8
Size: 432 123 570 613 2112 889 1629 43
Fragments arranged by size:
2112 1629 1425 981 948 889 888 613
570 432 275 123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 8497 1537
Size: 275 6685 4090
Fragments arranged by size:
6685 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2139 2236 2496 2578 2915 3129 3447 4155
Size: 786 97 260 82 337 214 318 708
Cuts at: 4155 4188 4275 4857 5780 6582 7476 8980 1353
Size: 33 87 582 923 802 894 1504 3423
Fragments arranged by size:
3423 1504 923 894 802 786 708 582
337 318 260 214 97 87 82 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3641 9637 10011 10157 387
Size: 1064 138 2052 5996 374 146 1280
Fragments arranged by size:
5996 2052 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2331 2506 3100 3423 3523 4597 4633 2331
Size: 175 594 323 100 1074 36 8748
Fragments arranged by size:
8748 1074 594 323 175 100 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2163 2520 2589 2681 2723 2729
Size: 393 446 85 357 69 92 42 6
Cuts at: 2729 2843 2912 2930 3110 3137 3341 3479 3596
Size: 114 69 18 180 27 204 138 117
Cuts at: 3596 3704 3842 3851 3860 3875 3953 4088 4100
Size: 108 138 9 9 15 78 135 12
Cuts at: 4100 4241 4325 4371 4583 4643 5090 5240 5264
Size: 141 84 46 212 60 447 150 24
Cuts at: 5264 5294 5390 5516 5640 5750 5819 5910 6179
Size: 30 96 126 124 110 69 91 269
Cuts at: 6179 6344 6539 6584 6740 6755 6938 6975 7016
Size: 165 195 45 156 15 183 37 41
Cuts at: 7016 7073 7281 7334 7382 7677 7826 7844 7899
Size: 57 208 53 48 295 149 18 55
Cuts at: 7899 8094 8137 9113 9710 10497 10765 1239
Size: 195 43 976 597 787 268 1524
Fragments arranged by size:
1524 976 787 597 447 446 393 357
295 269 268 212 208 204 195 195
183 180 165 156 150 149 141 138
138 135 126 124 117 114 110 108
96 92 91 85 84 78 69 69
69 60 57 55 53 48 46 45
43 42 41 37 30 27 24 18
18 15 15 12 9 9 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2435 3440 3790 4160 5072 5150 5732 5819 5897
Size: 1005 350 370 912 78 582 87 78
Cuts at: 5897 5939 8225 2435
Size: 42 2286 5260
Fragments arranged by size:
5260 2286 1005 912 582 370 350 87
78 78 42
EcoO109I rG'GnC_Cy
Cuts at: 2440 2905 3483 3646 4120 4686 5360 5725 6028
Size: 465 578 163 474 566 674 365 303
Cuts at: 6028 6067 6559 7200 7354 7686 7687 7993 7994
Size: 39 492 641 154 332 1 306 1
Cuts at: 7994 8218 2440
Size: 224 5272
Fragments arranged by size:
5272 674 641 578 566 492 474 465
365 332 306 303 224 163 154 39
1 1
EcoRI G'AATT_C
Cuts at: 8359 8737 8359
Size: 378 10672
EcoRV GAT'ATC
Cuts at: 2626 2626
Size: 11050
FspI TGC'GCA
Cuts at: 4775 7046 9556 4775
Size: 2271 2510 6269
Fragments arranged by size:
6269 2510 2271
HaeII r_GCGC'y
Cuts at: 1678 4491 4904 6769 7291 7497 8140 8295 10197
Size: 2813 413 1865 522 206 643 155 1902
Cuts at: 10197 1678
Size: 2531
Fragments arranged by size:
2813 2531 1902 1865 643 522 413 206
155
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2142 2174 4694 4726 5294
Size: 32 135 32 570 32 2520 32 568
Cuts at: 5294 5326 5690 5722 7801 7833 9691 9723 9765
Size: 32 364 32 2079 32 1858 32 42
Cuts at: 9765 9797 1373
Size: 32 2626
Fragments arranged by size:
2626 2520 2079 1858 570 568 364 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 8394 8640 669
Size: 988 281 1554 4902 246 3079
Fragments arranged by size:
4902 3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4694 5207 748
Size: 3946 513 6591
Fragments arranged by size:
6591 3946 513
HpaI GTT'AAC
Cuts at: 8640 8640
Size: 11050
KasI G'GCGC_C
Cuts at: 4487 8136 4487
Size: 3649 7401
KpnI G_GTAC'C
Cuts at: 8375 8375
Size: 11050
MfeI C'AATT_G
Cuts at: 8649 8649
Size: 11050
MluI A'CGCG_T
Cuts at: 10926 10926
Size: 11050
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3456 3646 6390 10164 10348 1305
Size: 2151 190 2744 3774 184 2007
Fragments arranged by size:
3774 2744 2151 2007 190 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3761 4649 5204 10
Size: 54 1425 343 1929 888 555 5856
Fragments arranged by size:
5856 1929 1425 888 555 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 6867 7457 9008 9367 9526 518
Size: 648 5701 590 1551 359 159 2042
Fragments arranged by size:
5701 2042 1551 648 590 359 159
MspA1I CmG'CkG
Cuts at: 1606 3088 3172 3193 3247 3394 3820 3931 4042
Size: 1482 84 21 54 147 426 111 111
Cuts at: 4042 4400 4507 5284 5917 6112 6131 6179 6214
Size: 358 107 777 633 195 19 48 35
Cuts at: 6214 6280 6313 6577 6781 6824 6907 6925 7156
Size: 66 33 264 204 43 83 18 231
Cuts at: 7156 7744 8028 8466 9143 10291 10536 1606
Size: 588 284 438 677 1148 245 2120
Fragments arranged by size:
2120 1482 1148 777 677 633 588 438
426 358 284 264 245 231 204 195
147 111 111 107 84 83 66 54
48 43 35 33 21 19 18
NaeI GCC'GGC
Cuts at: 4610 5654 6389 4610
Size: 1044 735 9271
Fragments arranged by size:
9271 1044 735
NarI GG'CG_CC
Cuts at: 4488 8137 4488
Size: 3649 7401
NcoI C'CATG_G
Cuts at: 513 1065 2134 2616 4380 513
Size: 552 1069 482 1764 7183
Fragments arranged by size:
7183 1764 1069 552 482
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11050
NgoMIV G'CCGG_C
Cuts at: 4608 5652 6387 4608
Size: 1044 735 9271
Fragments arranged by size:
9271 1044 735
NheI G'CTAG_C
Cuts at: 1052 2713 3607 7541 1052
Size: 1661 894 3934 4561
Fragments arranged by size:
4561 3934 1661 894
NotI GC'GGCC_GC
Cuts at: 8497 8497
Size: 11050
NspI r_CATG'y
Cuts at: 1132 1229 6322 7915 8408 9953 1132
Size: 97 5093 1593 493 1545 2229
Fragments arranged by size:
5093 2229 1593 1545 493 97
PasI CC'CwG_GG
Cuts at: 1348 2343 3643 7381 1348
Size: 995 1300 3738 5017
Fragments arranged by size:
5017 3738 1300 995
PciI A'CATG_T
Cuts at: 9949 9949
Size: 11050
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2349 3661 3757 5200 5728 5797 6442 8146
Size: 1241 1312 96 1443 528 69 645 1704
Cuts at: 8146 1108
Size: 4012
Fragments arranged by size:
4012 1704 1443 1312 1241 645 528 96
69
PfoI T'CCnGG_A
Cuts at: 2094 4104 4497 5727 5895 6561 6582 7395 7693
Size: 2010 393 1230 168 666 21 813 298
Cuts at: 7693 2094
Size: 5451
Fragments arranged by size:
5451 2010 1230 813 666 393 298 168
21
PmeI GTTT'AAAC
Cuts at: 8354 8354
Size: 11050
PmlI CAC'GTG
Cuts at: 2062 2062
Size: 11050
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5436 5468 9119 9151 10632 10664 5436
Size: 32 3651 32 1481 32 5822
Fragments arranged by size:
5822 3651 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 2905 5725 6559 7200 2440
Size: 465 2820 834 641 6290
Fragments arranged by size:
6290 2820 834 641 465
PshAI GACnn'nnGTC
Cuts at: 1613 4352 7404 1613
Size: 2739 3052 5259
Fragments arranged by size:
5259 3052 2739
PsiI TTA'TAA
Cuts at: 8620 8620
Size: 11050
PspOMI G'GGCC_C
Cuts at: 3483 4686 6207 7686 7993 3483
Size: 1203 1521 1479 307 6540
Fragments arranged by size:
6540 1521 1479 1203 307
PspXI vC'TCGA_Gb
Cuts at: 5453 6047 8325 5453
Size: 594 2278 8178
Fragments arranged by size:
8178 2278 594
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 7694 7726 7694
Size: 32 11018
PstI C_TGCA'G
Cuts at: 830 2091 2105 2211 2634 3009 3117 3198 3420
Size: 1261 14 106 423 375 108 81 222
Cuts at: 3420 3772 3867 4183 4554 4722 4889 4908 5109
Size: 352 95 316 371 168 167 19 201
Cuts at: 5109 5508 5823 5857 6045 6117 6843 6900 6930
Size: 399 315 34 188 72 726 57 30
Cuts at: 6930 7776 8402 830
Size: 846 626 3478
Fragments arranged by size:
3478 1261 846 726 626 423 399 375
371 352 316 315 222 201 188 168
167 108 106 95 81 72 57 34
30 19 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11050
PvuII CAG'CTG
Cuts at: 3088 3172 3193 3247 3394 3820 4042 4400 4507
Size: 84 21 54 147 426 222 358 107
Cuts at: 4507 5284 6112 6280 6313 6781 6824 6907 6925
Size: 777 828 168 33 468 43 83 18
Cuts at: 6925 7156 7744 8028 3088
Size: 231 588 284 6110
Fragments arranged by size:
6110 828 777 588 468 426 358 284
231 222 168 147 107 84 83 54
43 33 21 18
SacI G_AGCT'C
Cuts at: 2333 2508 3102 3425 3525 4599 4635 2333
Size: 175 594 323 100 1074 36 8748
Fragments arranged by size:
8748 1074 594 323 175 100 36
SalI G'TCGA_C
Cuts at: 1936 8392 1936
Size: 6456 4594
SanDI GG'GwC_CC
Cuts at: 7200 7200
Size: 11050
SapI GCTCTTCn'nnn_
Cuts at: 2496 2915 3447 4188 4857 5780 7476 2496
Size: 419 532 741 669 923 1696 6070
Fragments arranged by size:
6070 1696 923 741 669 532 419
SbfI CC_TGCA'GG
Cuts at: 3117 8402 3117
Size: 5285 5765
ScaI AGT'ACT
Cuts at: 9298 11010 9298
Size: 1712 9338
SexAI A'CCwGG_T
Cuts at: 3657 3657
Size: 11050
SfcI C'TryA_G
Cuts at: 826 1045 2087 2101 2207 2456 2555 2630 3005
Size: 219 1042 14 106 249 99 75 375
Cuts at: 3005 3014 3113 3194 3416 3768 3863 4179 4550
Size: 9 99 81 222 352 95 316 371
Cuts at: 4550 4718 4885 4904 5105 5504 5819 5853 6041
Size: 168 167 19 201 399 315 34 188
Cuts at: 6041 6113 6839 6896 6926 6980 7510 7772 8398
Size: 72 726 57 30 54 530 262 626
Cuts at: 8398 9533 10214 10405 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1042 726 681 626 530 399
375 371 352 316 315 262 249 222
219 201 191 188 168 167 106 99
99 95 81 75 72 57 54 34
30 19 14 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 5642 6125 5642
Size: 483 10567
SfoI GGC'GCC
Cuts at: 4489 8138 4489
Size: 3649 7401
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11050
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11050
SmaI CCC'GGG
Cuts at: 1872 2024 3896 4808 5474 5648 6422 6512 8377
Size: 152 1872 912 666 174 774 90 1865
Cuts at: 8377 1872
Size: 4545
Fragments arranged by size:
4545 1872 1865 912 774 666 174 152
90
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2837 3790 3812 5093 5453 6047
Size: 197 926 894 953 22 1281 360 594
Cuts at: 6047 7162 8325 9154 10055 10317 820
Size: 1115 1163 829 901 262 1553
Fragments arranged by size:
1553 1281 1163 1115 953 926 901 894
829 594 360 262 197 22
SnaBI TAC'GTA
Cuts at: 493 8841 493
Size: 8348 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11050
SphI G_CATG'C
Cuts at: 1132 6322 7915 8408 1132
Size: 5190 1593 493 3774
Fragments arranged by size:
5190 3774 1593 493
SspI AAT'ATT
Cuts at: 5 52 8974 5
Size: 47 8922 2081
Fragments arranged by size:
8922 2081 47
StuI AGG'CCT
Cuts at: 1517 3467 3638 3785 3974 7834 1517
Size: 1950 171 147 189 3860 4733
Fragments arranged by size:
4733 3860 1950 189 171 147
StyI C'CwwG_G
Cuts at: 513 1065 2134 2616 2829 3447 4146 4380 4767
Size: 552 1069 482 213 618 699 234 387
Cuts at: 4767 5595 6382 6549 7002 7299 8226 8331 8489
Size: 828 787 167 453 297 927 105 158
Cuts at: 8489 513
Size: 3074
Fragments arranged by size:
3074 1069 927 828 787 699 618 552
482 453 387 297 234 213 167 158
105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2035 2609 3221 6356 8073 8185 9077
Size: 1255 455 574 612 3135 1717 112 892
Cuts at: 9077 9094 9247 9432 9877 325
Size: 17 153 185 445 1498
Fragments arranged by size:
3135 1717 1498 1255 892 612 574 455
445 185 153 112 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 9296 11008 96
Size: 274 80 33 51 1443 7319 1712 138
Fragments arranged by size:
7319 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2180 2212 3603 3635 11010 11042 2180
Size: 32 1391 32 7375 32 2188
Fragments arranged by size:
7375 2188 1391 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 11050
XbaI T'CTAG_A
Cuts at: 8386 8386
Size: 11050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3454 4747 6218 7994 3454
Size: 1293 1471 1776 6510
Fragments arranged by size:
6510 1776 1471 1293
XhoI C'TCGA_G
Cuts at: 1943 5453 6047 8325 1943
Size: 3510 594 2278 4668
Fragments arranged by size:
4668 3510 2278 594
XmaI C'CCGG_G
Cuts at: 1870 2022 3894 4806 5472 5646 6420 6510 8375
Size: 152 1872 912 666 174 774 90 1865
Cuts at: 8375 1870
Size: 4545
Fragments arranged by size:
4545 1872 1865 912 774 666 174 152
90
XmnI GAAnn'nnTTC
Cuts at: 3150 3163 6011 7706 9179 3150
Size: 13 2848 1695 1473 5021
Fragments arranged by size:
5021 2848 1695 1473 13
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7390 8858 276
Size: 53 83 186 936 5856 1468 2468
Fragments arranged by size:
5856 2468 1468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BstZ17I Bsu36I FalI
FseI FspAI NruI NsiI PacI RsrII SacII SrfI
SwaI