Restriction map for
pFN21AB0019
(Circular) (Six-base) MAPSORT of: pFN21AB0019.seq Check: 1365 from: 1 to: 13234
pFN21AB0019 13234 bp
With 182 enzymes: SgfI *
January 31, 2011 17:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 9958 9958
Size: 13234
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 11044 278
Size: 53 83 186 936 9508 2468
Fragments arranged by size:
9508 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2695 3988 4727 8959 9115 10577 1937
Size: 758 1293 739 4232 156 1462 4594
Fragments arranged by size:
4594 4232 1462 1293 758 739 156
Acc65I G'GTAC_C
Cuts at: 2618 10555 2618
Size: 7937 5297
AclI AA'CG_TT
Cuts at: 1563 5440 11361 11734 1563
Size: 3877 5921 373 3063
Fragments arranged by size:
5921 3877 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2072 2474 4986 5469 5874 5973 6018 6054
Size: 440 402 2512 483 405 99 45 36
Cuts at: 6054 7769 8127 10383 11297 12681 12949 1632
Size: 1715 358 2256 914 1384 268 1917
Fragments arranged by size:
2512 2256 1917 1715 1384 914 483 440
405 402 358 268 99 45 36
AflII C'TTAA_G
Cuts at: 820 1017 8762 9358 820
Size: 197 7745 596 4696
Fragments arranged by size:
7745 4696 596 197
AflIII A'CryG_T
Cuts at: 1203 4841 7364 7366 9775 12133 13110 1203
Size: 3638 2523 2 2409 2358 977 1327
Fragments arranged by size:
3638 2523 2409 2358 1327 977 2
AgeI A'CCGG_T
Cuts at: 12065 12065
Size: 13234
AhdI GACnn_n'nnGTC
Cuts at: 11963 11963
Size: 13234
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 13234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3411 3651 7281 1184
Size: 2227 240 3630 7137
Fragments arranged by size:
7137 3630 2227 240
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10477 10509 10477
Size: 32 13202
AlwNI CAG_nnn'CTG
Cuts at: 3326 5897 6458 7844 9086 10977 12549 3326
Size: 2571 561 1386 1242 1891 1572 4011
Fragments arranged by size:
4011 2571 1891 1572 1386 1242 561
ApaLI G'TGCA_C
Cuts at: 3837 11291 12447 3837
Size: 7454 1156 4624
Fragments arranged by size:
7454 4624 1156
ApoI r'AATT_y
Cuts at: 1493 2533 2664 3118 3652 3784 4828 6062 7052
Size: 1040 131 454 534 132 1044 1234 990
Cuts at: 7052 7447 7861 8296 8329 8502 8628 9860 10543
Size: 395 414 435 33 173 126 1232 683
Cuts at: 10543 10769 10921 13030 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1234 1232 1044 1040 990 683
534 454 435 414 395 226 173 152
132 131 126 33
AseI AT'TA_AT
Cuts at: 160 1032 5367 7188 11788 160
Size: 872 4335 1821 4600 1606
Fragments arranged by size:
4600 4335 1821 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13234
AvaI C'yCGr_G
Cuts at: 1870 1943 3123 3141 8679 9681 10559 1870
Size: 73 1180 18 5538 1002 878 4545
Fragments arranged by size:
5538 4545 1180 1002 878 73 18
AvrII C'CTAG_G
Cuts at: 10373 10373
Size: 13234
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 8043 8076 8043
Size: 33 13201
BamHI G'GATC_C
Cuts at: 10564 10931 10564
Size: 367 12867
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2618 9946 10555 12010 618
Size: 325 217 627 831 7328 609 1455 1842
Fragments arranged by size:
7328 1842 1455 831 627 609 325 217
BanII G_rGCy'C
Cuts at: 1391 7980 10553 1391
Size: 6589 2573 4072
Fragments arranged by size:
6589 4072 2573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2510 3053 3624 4716 5631 7263 7462
Size: 971 611 543 571 1092 915 1632 199
Cuts at: 7462 9255 9571 928
Size: 1793 316 4591
Fragments arranged by size:
4591 1793 1632 1092 971 915 611 571
543 316 199
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 11425 11459 1903
Size: 34 9488 34 3678
Fragments arranged by size:
9488 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9832 11126 12336 9832
Size: 1294 1210 10730
Fragments arranged by size:
10730 1294 1210
BclI T'GATC_A
Cuts at: 1548 2023 8461 1548
Size: 475 6438 6321
Fragments arranged by size:
6438 6321 475
BfrBI ATG'CAT
Cuts at: 4043 7185 4043
Size: 3142 10092
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3551 11845 136
Size: 107 122 71 631 605 1879 8294 1525
Fragments arranged by size:
8294 1879 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3339 4641 4877 8227 10013 10022 13229 3339
Size: 1302 236 3350 1786 9 3207 3344
Fragments arranged by size:
3350 3344 3207 1786 1302 236 9
BlpI GC'TnA_GC
Cuts at: 2775 4302 10651 2775
Size: 1527 6349 5358
Fragments arranged by size:
6349 5358 1527
Bme1580I G_kGCm'C
Cuts at: 1790 3841 7042 11295 12451 1790
Size: 2051 3201 4253 1156 2573
Fragments arranged by size:
4253 3201 2573 2051 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 13234
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6258 7801 8051
Size: 416 53 83 399 24 4841 1543 250
Cuts at: 8051 10965 11923 442
Size: 2914 958 1753
Fragments arranged by size:
4841 2914 1753 1543 958 416 399 250
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3011 1056
Size: 1955 11279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2757 9211 2757
Size: 6454 6780
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2274 2346 6477 6567 7038 8754 9093 9432
Size: 1035 72 4131 90 471 1716 339 339
Cuts at: 9432 10410 11894 1239
Size: 978 1484 2579
Fragments arranged by size:
4131 2579 1716 1484 1035 978 471 339
339 90 72
Bpu10I CC'TnA_GC
Cuts at: 6405 10497 6405
Size: 4092 9142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2435 4109 4228 5249 7089 7995 8053 8156 8710
Size: 1674 119 1021 1840 906 58 103 554
Cuts at: 8710 10288 11359 12224 12522 2435
Size: 1578 1071 865 298 3147
Fragments arranged by size:
3147 1840 1674 1578 1071 1021 906 865
554 298 119 103 58
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5470 6134 8251 9600 11897 882
Size: 617 3971 664 2117 1349 2297 2219
Fragments arranged by size:
3971 2297 2219 2117 1349 664 617
BsaAI yAC'GTr
Cuts at: 493 1204 6770 7367 11025 493
Size: 711 5566 597 3658 2702
Fragments arranged by size:
5566 3658 2702 711 597
BsaBI GATnn'nnATC
Cuts at: 2311 8911 13228 2311
Size: 6600 4317 2317
Fragments arranged by size:
6600 4317 2317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 11041 11423 12058 275
Size: 53 83 186 936 9508 382 635 1451
Fragments arranged by size:
9508 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3870 7882 11667 12065 12339 12486 12897 3870
Size: 4012 3785 398 274 147 411 4207
Fragments arranged by size:
4207 4012 3785 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 7011 7041 8797 8827 1178
Size: 30 165 30 5608 30 1756 30 5585
Fragments arranged by size:
5608 5585 1756 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3059 4495 6321 7047 8713 8835 1186
Size: 1873 1436 1826 726 1666 122 5585
Fragments arranged by size:
5585 1873 1826 1666 1436 726 122
BseYI C'CCAG_C
Cuts at: 4499 5005 8943 12437 12933 4499
Size: 506 3938 3494 496 4800
Fragments arranged by size:
4800 3938 3494 506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2353 2886 3177 5694 7263 1163
Size: 573 617 533 291 2517 1569 7134
Fragments arranged by size:
7134 2517 1569 617 573 533 291
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 10684 11445 12473 1540
Size: 275 186 8683 761 1028 2301
Fragments arranged by size:
8683 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2647 3589 3841 5249 6103 6529 9657 11295 11380
Size: 942 252 1408 854 426 3128 1638 85
Cuts at: 11380 12451 2647
Size: 1071 3430
Fragments arranged by size:
3430 3128 1638 1408 1071 942 854 426
252 85
BsmI GAATG_Cn'
Cuts at: 7187 9169 10745 10838 7187
Size: 1982 1576 93 9583
Fragments arranged by size:
9583 1982 1576 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2647 3589 3841 5249 6103 6529 7042
Size: 399 857 942 252 1408 854 426 513
Cuts at: 7042 7980 9657 10553 11295 11380 12451 1391
Size: 938 1677 896 742 85 1071 2174
Fragments arranged by size:
2174 1677 1408 1071 942 938 896 857
854 742 513 426 399 252 85
BspEI T'CCGG_A
Cuts at: 12897 12897
Size: 13234
BspHI T'CATG_A
Cuts at: 2977 3283 11123 12853 2977
Size: 306 7840 1730 3358
Fragments arranged by size:
7840 3358 1730 306
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2630 3619 8947 9958 10589 844
Size: 1786 989 5328 1011 631 3489
Fragments arranged by size:
5328 3489 1786 1011 989 631
BsrBI CCG'CTC
Cuts at: 1394 1823 2779 11121 1394
Size: 429 956 8342 3507
Fragments arranged by size:
8342 3507 956 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2429 3097 3405 3426 3796 8148 11729
Size: 1183 1181 668 308 21 370 4352 3581
Cuts at: 11729 11903 65
Size: 174 1396
Fragments arranged by size:
4352 3581 1396 1183 1181 668 370 308
174 21
BsrFI r'CCGG_y
Cuts at: 1700 1810 2759 11878 12065 1700
Size: 110 949 9119 187 2869
Fragments arranged by size:
9119 2869 949 187 110
BsrGI T'GTAC_A
Cuts at: 96 6846 96
Size: 6750 6484
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 13234
BssSI C'ACGA_G
Cuts at: 11294 12306 11294
Size: 1012 12222
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5897 6380 6422 794
Size: 5103 483 42 7606
Fragments arranged by size:
7606 5103 483 42
BstBI TT'CG_AA
Cuts at: 8713 12038 8713
Size: 3325 9909
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 13234
BstXI CCAn_nnnn'nTGG
Cuts at: 4675 8206 8882 4675
Size: 3531 676 9027
Fragments arranged by size:
9027 3531 676
BstYI r'GATC_y
Cuts at: 1972 2306 3339 4641 4877 5956 8227 8912 10013
Size: 334 1033 1302 236 1079 2271 685 1101
Cuts at: 10013 10022 10416 10564 10931 11316 11333 12785 12871
Size: 9 394 148 367 385 17 1452 86
Cuts at: 12871 12883 13229 1972
Size: 12 346 1977
Fragments arranged by size:
2271 1977 1452 1302 1101 1079 1033 685
394 385 367 346 334 236 148 86
17 12 9
BstZ17I GTA'TAC
Cuts at: 2696 2696
Size: 13234
Bsu36I CC'TnA_GG
Cuts at: 9079 9898 9079
Size: 819 12415
BtgI C'CryG_G
Cuts at: 513 1065 2004 6480 6920 10149 513
Size: 552 939 4476 440 3229 3598
Fragments arranged by size:
4476 3598 3229 939 552 440
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2615 487
Size: 683 56 1389 11106
Fragments arranged by size:
11106 1389 683 56
BtsI GCAGTG_nn'
Cuts at: 4650 5653 5985 6241 10138 10750 11544 11564 4650
Size: 1003 332 256 3897 612 794 20 6320
Fragments arranged by size:
6320 3897 1003 794 612 332 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 13234
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5328 5363 529
Size: 35 4764 35 8400
Fragments arranged by size:
8400 4764 35 35
DraI TTT'AAA
Cuts at: 4896 5547 7401 10538 4896
Size: 651 1854 3137 7592
Fragments arranged by size:
7592 3137 1854 651
DraIII CAC_nnn'GTG
Cuts at: 10325 10325
Size: 13234
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 12241 809
Size: 507 10925 1802
Fragments arranged by size:
10925 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 8977 10406 10681
Size: 54 1425 50 275 18 7147 1429 275
Cuts at: 10681 11570 13199 8
Size: 889 1629 43
Fragments arranged by size:
7147 1629 1429 1425 889 275 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 10681 1537
Size: 275 8869 4090
Fragments arranged by size:
8869 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2359 3378 4263 4513 5466 5502 11164 1353
Size: 1006 1019 885 250 953 36 5662 3423
Fragments arranged by size:
5662 3423 1019 1006 953 885 250 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 11821 12195 12341 387
Size: 1064 138 10232 374 146 1280
Fragments arranged by size:
10232 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2072 2274 2346 2474 4986 5469 5874
Size: 393 440 202 72 128 2512 483 405
Cuts at: 5874 5973 6018 6054 6477 6567 7038 7769 8127
Size: 99 45 36 423 90 471 731 358
Cuts at: 8127 8754 9093 9432 10383 10410 11297 11894 12681
Size: 627 339 339 951 27 887 597 787
Cuts at: 12681 12949 1239
Size: 268 1524
Fragments arranged by size:
2512 1524 951 887 787 731 627 597
483 471 440 423 405 393 358 339
339 268 202 128 99 90 72 45
36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 7080 7551 8120 8790 7080
Size: 471 569 670 11524
Fragments arranged by size:
11524 670 569 471
EcoO109I rG'GnC_Cy
Cuts at: 6688 9432 6688
Size: 2744 10490
EcoRI G'AATT_C
Cuts at: 2664 8502 10543 10921 2664
Size: 5838 2041 378 4977
Fragments arranged by size:
5838 4977 2041 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5241 5403 7536 9732 5241
Size: 162 2133 2196 8743
Fragments arranged by size:
8743 2196 2133 162
FspI TGC'GCA
Cuts at: 11740 11740
Size: 13234
HaeII r_GCGC'y
Cuts at: 1678 9580 10504 12381 1678
Size: 7902 924 1877 2531
Fragments arranged by size:
7902 2531 1877 924
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2723 2755 2908 2940 2978
Size: 32 135 32 1151 32 153 32 38
Cuts at: 2978 3010 3086 3118 4418 4450 5642 5674 5706
Size: 32 76 32 1300 32 1192 32 32
Cuts at: 5706 9609 9641 10408 10440 11875 11907 11949 11981
Size: 3903 32 767 32 1435 32 42 32
Cuts at: 11981 1373
Size: 2626
Fragments arranged by size:
3903 2626 1435 1300 1192 1151 767 153
135 76 42 38 32 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 669 1657 1938 7621 9327 10578 10824 669
Size: 988 281 5683 1706 1251 246 3079
Fragments arranged by size:
5683 3079 1706 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4246 4522 8385 748
Size: 3498 276 3863 5597
Fragments arranged by size:
5597 3863 3498 276
HpaI GTT'AAC
Cuts at: 10824 10824
Size: 13234
KpnI G_GTAC'C
Cuts at: 2622 10559 2622
Size: 7937 5297
MfeI C'AATT_G
Cuts at: 2567 10833 2567
Size: 8266 4968
MluI A'CGCG_T
Cuts at: 13110 13110
Size: 13234
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3682 4803 5296 7734 12348 12532 1305
Size: 2377 1121 493 2438 4614 184 2007
Fragments arranged by size:
4614 2438 2377 2007 1121 493 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 11412
Fragments arranged by size:
11412 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3090 3393 5046 11192 11551 11710 518
Size: 648 1924 303 1653 6146 359 159 2042
Fragments arranged by size:
6146 2042 1924 1653 648 359 303 159
MspA1I CmG'CkG
Cuts at: 1606 3033 5852 5951 6838 10650 11327 12475 12720
Size: 1427 2819 99 887 3812 677 1148 245
Cuts at: 12720 1606
Size: 2120
Fragments arranged by size:
3812 2819 2120 1427 1148 887 677 245
99
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 11743
Fragments arranged by size:
11743 939 552
NdeI CA'TA_TG
Cuts at: 387 2020 5043 5634 387
Size: 1633 3023 591 7987
Fragments arranged by size:
7987 3023 1633 591
NheI G'CTAG_C
Cuts at: 1052 3007 1052
Size: 1955 11279
NotI GC'GGCC_GC
Cuts at: 10681 10681
Size: 13234
NruI TCG'CGA
Cuts at: 2998 2998
Size: 13234
NsiI A_TGCA'T
Cuts at: 4045 7187 4045
Size: 3142 10092
NspI r_CATG'y
Cuts at: 1132 1229 3398 4047 4123 4845 6893 8373 8668
Size: 97 2169 649 76 722 2048 1480 295
Cuts at: 8668 9779 10592 12137 1132
Size: 1111 813 1545 2229
Fragments arranged by size:
2229 2169 2048 1545 1480 1111 813 722
649 295 97 76
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 13234
PciI A'CATG_T
Cuts at: 4841 9775 12133 4841
Size: 4934 2358 5942
Fragments arranged by size:
5942 4934 2358
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2632 5282 6236 1108
Size: 1524 2650 954 8106
Fragments arranged by size:
8106 2650 1524 954
PfoI T'CCnGG_A
Cuts at: 4612 8704 4612
Size: 4092 9142
PmeI GTTT'AAAC
Cuts at: 10538 10538
Size: 13234
PmlI CAC'GTG
Cuts at: 7367 7367
Size: 13234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 11303 11335 12816 12848 11303
Size: 32 1481 32 11689
Fragments arranged by size:
11689 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 6688 9432 6688
Size: 2744 10490
PshAI GACnn'nnGTC
Cuts at: 1613 4031 1613
Size: 2418 10816
PsiI TTA'TAA
Cuts at: 6257 8589 10804 6257
Size: 2332 2215 8687
Fragments arranged by size:
8687 2332 2215
PspXI vC'TCGA_Gb
Cuts at: 9681 9681
Size: 13234
PstI C_TGCA'G
Cuts at: 830 2014 4177 4657 6018 6418 6430 10586 830
Size: 1184 2163 480 1361 400 12 4156 3478
Fragments arranged by size:
4156 3478 2163 1361 1184 480 400 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 13234
PvuII CAG'CTG
Cuts at: 3033 5852 5951 6838 3033
Size: 2819 99 887 9429
Fragments arranged by size:
9429 2819 887 99
SalI G'TCGA_C
Cuts at: 1936 10576 1936
Size: 8640 4594
SapI GCTCTTCn'nnn_
Cuts at: 3378 3378
Size: 13234
SbfI CC_TGCA'GG
Cuts at: 10586 10586
Size: 13234
ScaI AGT'ACT
Cuts at: 2145 2395 6490 9061 9880 11482 13194 2145
Size: 250 4095 2571 819 1602 1712 2185
Fragments arranged by size:
4095 2571 2185 1712 1602 819 250
SexAI A'CCwGG_T
Cuts at: 7248 7248
Size: 13234
SfcI C'TryA_G
Cuts at: 826 1045 2010 3419 3481 3828 4173 4488 4653
Size: 219 965 1409 62 347 345 315 165
Cuts at: 4653 6014 6414 6426 7077 9546 10269 10582 11717
Size: 1361 400 12 651 2469 723 313 1135
Cuts at: 11717 12398 12589 826
Size: 681 191 1471
Fragments arranged by size:
2469 1471 1409 1361 1135 965 723 681
651 400 347 345 315 313 219 191
165 62 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 13234
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 13234
SmaI CCC'GGG
Cuts at: 1872 3125 10561 1872
Size: 1253 7436 4545
Fragments arranged by size:
7436 4545 1253
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2414 4124 4243 5264 7104 8010
Size: 197 926 471 1710 119 1021 1840 906
Cuts at: 8010 8068 8171 8679 8725 8762 9358 9681 10303
Size: 58 103 508 46 37 596 323 622
Cuts at: 10303 11338 12239 12501 820
Size: 1035 901 262 1553
Fragments arranged by size:
1840 1710 1553 1035 1021 926 906 901
622 596 508 471 323 262 197 119
103 58 46 37
SnaBI TAC'GTA
Cuts at: 493 11025 493
Size: 10532 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 13234
SphI G_CATG'C
Cuts at: 1132 4123 10592 1132
Size: 2991 6469 3774
Fragments arranged by size:
6469 3774 2991
SspI AAT'ATT
Cuts at: 5 52 4228 5096 11158 5
Size: 47 4176 868 6062 2081
Fragments arranged by size:
6062 4176 2081 868 47
StuI AGG'CCT
Cuts at: 1517 4204 1517
Size: 2687 10547
StyI C'CwwG_G
Cuts at: 513 1065 2004 2934 4347 7464 9041 10373 10673
Size: 552 939 930 1413 3117 1577 1332 300
Cuts at: 10673 513
Size: 3074
Fragments arranged by size:
3117 3074 1577 1413 1332 939 930 552
300
SwaI ATTT'AAAT
Cuts at: 7401 7401
Size: 13234
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 6124 7297 8198 9967 11261 11278 11431
Size: 1255 4544 1173 901 1769 1294 17 153
Cuts at: 11431 11616 12061 325
Size: 185 445 1498
Fragments arranged by size:
4544 1769 1498 1294 1255 1173 901 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2143 2393 4212
Size: 274 80 33 51 1443 166 250 1819
Cuts at: 4212 5219 6164 6488 6846 7091 8525 8729 9059
Size: 1007 945 324 358 245 1434 204 330
Cuts at: 9059 9878 11480 13192 96
Size: 819 1602 1712 138
Fragments arranged by size:
1819 1712 1602 1443 1434 1007 945 819
358 330 324 274 250 245 204 166
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2922 2954 5548 5580 8797 8829 13194 13226 2922
Size: 32 2594 32 3217 32 4365 32 2930
Fragments arranged by size:
4365 3217 2930 2594 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 7250 1316
Size: 5934 7300
XbaI T'CTAG_A
Cuts at: 7597 8378 8903 8915 9254 9410 9614 10570 7597
Size: 781 525 12 339 156 204 956 10261
Fragments arranged by size:
10261 956 781 525 339 204 156 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2566 2941 3083 5279 7138 10147 2566
Size: 375 142 2196 1859 3009 5653
Fragments arranged by size:
5653 3009 2196 1859 375 142
XhoI C'TCGA_G
Cuts at: 1943 8679 9681 1943
Size: 6736 1002 5496
Fragments arranged by size:
6736 5496 1002
XmaI C'CCGG_G
Cuts at: 1870 3123 10559 1870
Size: 1253 7436 4545
Fragments arranged by size:
7436 4545 1253
XmnI GAAnn'nnTTC
Cuts at: 4621 7582 8077 9262 11363 4621
Size: 2961 495 1185 2101 6492
Fragments arranged by size:
6492 2961 2101 1185 495
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 11042 276
Size: 53 83 186 936 9508 2468
Fragments arranged by size:
9508 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NruI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI BbeI BbvCI BsiWI BsmBI EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV PacI
PspOMI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI