Restriction map for
pFN21AB0016
(Circular) (Six-base) MAPSORT of: pFN21AB0016.seq Check: 1607 from: 1 to: 9775
pFN21AB0016 9775 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3793 7585 278
Size: 53 83 186 936 2257 3792 2468
Fragments arranged by size:
3792 2468 2257 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 6873 7118 1937
Size: 1149 3787 245 4594
Fragments arranged by size:
4594 3787 1149 245
Acc65I G'GTAC_C
Cuts at: 7096 7096
Size: 9775
AclI AA'CG_TT
Cuts at: 1563 7902 8275 1563
Size: 6339 373 3063
Fragments arranged by size:
6339 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2339 2705 2732 3077 3768 3954 4706 5687
Size: 707 366 27 345 691 186 752 981
Cuts at: 5687 5748 6453 6492 7838 9222 9490 1632
Size: 61 705 39 1346 1384 268 1917
Fragments arranged by size:
1917 1384 1346 981 752 707 705 691
366 345 268 186 61 39 27
AflII C'TTAA_G
Cuts at: 820 1017 5189 820
Size: 197 4172 5406
Fragments arranged by size:
5406 4172 197
AflIII A'CryG_T
Cuts at: 1203 2662 4049 4070 6171 8674 9651 1203
Size: 1459 1387 21 2101 2503 977 1327
Fragments arranged by size:
2503 2101 1459 1387 1327 977 21
AgeI A'CCGG_T
Cuts at: 8606 8606
Size: 9775
AhdI GACnn_n'nnGTC
Cuts at: 8504 8504
Size: 9775
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9775
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 6862 1184
Size: 5678 4097
AlwNI CAG_nnn'CTG
Cuts at: 2233 2684 4859 5224 6038 7518 9090 2233
Size: 451 2175 365 814 1480 1572 2918
Fragments arranged by size:
2918 2175 1572 1480 814 451 365
ApaI G_GGCC'C
Cuts at: 2742 2742
Size: 9775
ApaLI G'TGCA_C
Cuts at: 7832 8988 7832
Size: 1156 8619
ApoI r'AATT_y
Cuts at: 1493 2154 2291 2496 3182 3334 5197 5489 5630
Size: 661 137 205 686 152 1863 292 141
Cuts at: 5630 7084 7310 7462 9571 1493
Size: 1454 226 152 2109 1697
Fragments arranged by size:
2109 1863 1697 1454 686 661 292 226
205 152 152 141 137
AseI AT'TA_AT
Cuts at: 160 1032 3381 8329 160
Size: 872 2349 4948 1606
Fragments arranged by size:
4948 2349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9775
AvaI C'yCGr_G
Cuts at: 1870 1943 3048 3572 5007 5868 7100 1870
Size: 73 1105 524 1435 861 1232 4545
Fragments arranged by size:
4545 1435 1232 1105 861 524 73
BamHI G'GATC_C
Cuts at: 5603 7105 7472 5603
Size: 1502 367 7906
Fragments arranged by size:
7906 1502 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3822 4801 6280 7096 8551
Size: 325 217 627 2035 979 1479 816 1455
Cuts at: 8551 618
Size: 1842
Fragments arranged by size:
2035 1842 1479 1455 979 816 627 325
217
BanII G_rGCy'C
Cuts at: 1391 2742 4439 4649 4848 5231 5541 6275 7094
Size: 1351 1697 210 199 383 310 734 819
Cuts at: 7094 1391
Size: 4072
Fragments arranged by size:
4072 1697 1351 819 734 383 310 210
199
BbeI G_GCGC'C
Cuts at: 3826 3826
Size: 9775
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2675 2803 4179 4502 6051 6441 928
Size: 971 776 128 1376 323 1549 390 4262
Fragments arranged by size:
4262 1549 1376 971 776 390 323 128
BbvCI CC'TCA_GC
Cuts at: 4314 4314
Size: 9775
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7966 8000 1903
Size: 34 6029 34 3678
Fragments arranged by size:
6029 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5707 7667 8877 5707
Size: 1960 1210 6605
Fragments arranged by size:
6605 1960 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9775
BfrBI ATG'CAT
Cuts at: 5428 5428
Size: 9775
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8386 136
Size: 107 122 71 631 605 6714 1525
Fragments arranged by size:
6714 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2563 6361 9770 2563
Size: 3798 3409 2568
Fragments arranged by size:
3798 3409 2568
BlpI GC'TnA_GC
Cuts at: 2102 4398 5225 7192 2102
Size: 2296 827 1967 4685
Fragments arranged by size:
4685 2296 1967 827
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2742 2997 4127 4213 6285 7836 8992
Size: 273 679 255 1130 86 2072 1551 1156
Cuts at: 8992 1790
Size: 2573
Fragments arranged by size:
2573 2072 1551 1156 1130 679 273 255
86
BmgBI CAC'GTC
Cuts at: 1318 2663 1318
Size: 1345 8430
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2475 3502 5184
Size: 416 53 83 399 24 1058 1027 1682
Cuts at: 5184 6886 7506 8464 442
Size: 1702 620 958 1753
Fragments arranged by size:
1753 1702 1682 1058 1027 958 620 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9775
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6249 6249
Size: 9775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2706 3028 3487 3566 4610 5345 5363 6285
Size: 1467 322 459 79 1044 735 18 922
Cuts at: 6285 6329 6342 6921 8435 1239
Size: 44 13 579 1514 2579
Fragments arranged by size:
2579 1514 1467 1044 922 735 579 459
322 79 44 18 13
Bpu10I CC'TnA_GC
Cuts at: 4086 4314 4584 4881 6327 4086
Size: 228 270 297 1446 7534
Fragments arranged by size:
7534 1446 297 270 228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 5093 6219 7900 8765 9063 2264
Size: 2829 1126 1681 865 298 2976
Fragments arranged by size:
2976 2829 1681 1126 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2475 4373 5661 7061 8438 882
Size: 617 976 1898 1288 1400 1377 2219
Fragments arranged by size:
2219 1898 1400 1377 1288 976 617
BsaAI yAC'GTr
Cuts at: 493 1204 7566 493
Size: 711 6362 2702
Fragments arranged by size:
6362 2702 711
BsaBI GATnn'nnATC
Cuts at: 6143 9769 6143
Size: 3626 6149
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3790 3814 3823 5095
Size: 53 83 186 936 2257 24 9 1272
Cuts at: 5095 7582 7964 8599 275
Size: 2487 382 635 1451
Fragments arranged by size:
2487 2257 1451 1272 936 635 382 186
83 53 24 9
BsaWI w'CCGG_w
Cuts at: 3271 8208 8606 8880 9027 9438 3271
Size: 4937 398 274 147 411 3608
Fragments arranged by size:
4937 3608 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4830 4860 1178
Size: 30 165 30 3427 30 6093
Fragments arranged by size:
6093 3427 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 3235 3563 5988 6170 6821 1186
Size: 913 1136 328 2425 182 651 4140
Fragments arranged by size:
4140 2425 1136 913 651 328 182
BseYI C'CCAG_C
Cuts at: 2735 3588 8978 9474 2735
Size: 853 5390 496 3036
Fragments arranged by size:
5390 3036 853 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2567 5916 6216 6648 1163
Size: 573 831 3349 300 432 4290
Fragments arranged by size:
4290 3349 831 573 432 300
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2116 2162 7225 7986 9014 1540
Size: 275 186 115 46 5063 761 1028 2301
Fragments arranged by size:
5063 2301 1028 761 275 186 115 46
BsiHKAI G_wGCw'C
Cuts at: 4585 4848 5541 6743 7836 7921 8992 4585
Size: 263 693 1202 1093 85 1071 5368
Fragments arranged by size:
5368 1202 1093 1071 693 263 85
BsiWI C'GTAC_G
Cuts at: 5716 5716
Size: 9775
BsmI GAATG_Cn'
Cuts at: 3884 5428 6829 7286 7379 3884
Size: 1544 1401 457 93 6280
Fragments arranged by size:
6280 1544 1401 457 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2742 2997 4127 4213 4439 4585
Size: 399 273 679 255 1130 86 226 146
Cuts at: 4585 4649 4848 5231 5541 6275 6285 6743 7094
Size: 64 199 383 310 734 10 458 351
Cuts at: 7094 7836 7921 8992 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1130 1071 742 734 679 458 399
383 351 310 273 255 226 199 146
86 85 64 10
BspEI T'CCGG_A
Cuts at: 9438 9438
Size: 9775
BspHI T'CATG_A
Cuts at: 3621 3927 4573 4767 7664 9394 3621
Size: 306 646 194 2897 1730 4002
Fragments arranged by size:
4002 2897 1730 646 306 194
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 7130 844
Size: 6286 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2101 4024 4090 7662 1394
Size: 429 278 1923 66 3572 3507
Fragments arranged by size:
3572 3507 1923 429 278 66
BsrDI GCAATG_nn'
Cuts at: 65 1248 2256 2766 4833 8270 8444 65
Size: 1183 1008 510 2067 3437 174 1396
Fragments arranged by size:
3437 2067 1396 1183 1008 510 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2141 8419 8606 1700
Size: 110 331 6278 187 2869
Fragments arranged by size:
6278 2869 331 187 110
BsrGI T'GTAC_A
Cuts at: 96 3660 3964 96
Size: 3564 304 5907
Fragments arranged by size:
5907 3564 304
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9775
BssSI C'ACGA_G
Cuts at: 3705 3842 7835 8847 3705
Size: 137 3993 1012 4633
Fragments arranged by size:
4633 3993 1012 137
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3652 4256 6203 794
Size: 2858 604 1947 4366
Fragments arranged by size:
4366 2858 1947 604
BstBI TT'CG_AA
Cuts at: 4348 8579 4348
Size: 4231 5544
BstEII G'GTnAC_C
Cuts at: 1184 2401 2788 6163 1184
Size: 1217 387 3375 4796
Fragments arranged by size:
4796 3375 1217 387
BstXI CCAn_nnnn'nTGG
Cuts at: 2749 3510 5383 2749
Size: 761 1873 7141
Fragments arranged by size:
7141 1873 761
BstYI r'GATC_y
Cuts at: 1972 2095 2309 2322 2350 2563 3871 4741 5314
Size: 123 214 13 28 213 1308 870 573
Cuts at: 5314 5603 6361 7057 7105 7472 7857 7874 9326
Size: 289 758 696 48 367 385 17 1452
Cuts at: 9326 9412 9424 9770 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1308 870 758 696 573 385
367 346 289 214 213 123 86 48
28 17 13 12
BstZ17I GTA'TAC
Cuts at: 3087 3087
Size: 9775
Bsu36I CC'TnA_GG
Cuts at: 2586 3818 2586
Size: 1232 8543
BtgI C'CryG_G
Cuts at: 513 1065 2004 3410 3903 5376 513
Size: 552 939 1406 493 1473 4912
Fragments arranged by size:
4912 1473 1406 939 552 493
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9036
Fragments arranged by size:
9036 683 56
BtsI GCAGTG_nn'
Cuts at: 4034 5120 5750 5989 7291 8085 8105 4034
Size: 1086 630 239 1302 794 20 5704
Fragments arranged by size:
5704 1302 1086 794 630 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9775
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9740
DraI TTT'AAA
Cuts at: 7079 7079
Size: 9775
DraIII CAC_nnn'GTG
Cuts at: 4915 4915
Size: 9775
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4229 5683 8782 809
Size: 507 2913 1454 3099 1802
Fragments arranged by size:
3099 2913 1802 1454 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2113 2159 5823
Size: 54 1425 50 275 18 283 46 3664
Cuts at: 5823 6657 7222 8111 9740 8
Size: 834 565 889 1629 43
Fragments arranged by size:
3664 1629 1425 889 834 565 283 275
54 50 46 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2113 2159 7222 1537
Size: 275 301 46 5063 4090
Fragments arranged by size:
5063 4090 301 275 46
EarI CTCTTCn'nnn_
Cuts at: 1353 2645 3303 3442 4560 5580 6129 6263 6468
Size: 1292 658 139 1118 1020 549 134 205
Cuts at: 6468 6511 6675 7705 1353
Size: 43 164 1030 3423
Fragments arranged by size:
3423 1292 1118 1030 1020 658 549 205
164 139 134 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 2147 3301 5194 6418 8362
Size: 1064 138 462 96 1154 1893 1224 1944
Cuts at: 8362 8736 8882 387
Size: 374 146 1280
Fragments arranged by size:
1944 1893 1280 1224 1154 1064 462 374
146 138 96
EcoICRI GAG'CTC
Cuts at: 4846 5539 4846
Size: 693 9082
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2339 2705 2706 2732 3028 3077 3487
Size: 393 707 366 1 26 296 49 410
Cuts at: 3487 3566 3768 3954 4610 4706 5345 5363 5687
Size: 79 202 186 656 96 639 18 324
Cuts at: 5687 5748 6285 6329 6342 6453 6492 6921 7838
Size: 61 537 44 13 111 39 429 917
Cuts at: 7838 8435 9222 9490 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 917 787 707 656 639 597 537
429 410 393 366 324 296 268 202
186 111 96 79 61 49 44 39
26 18 13 1
EcoO109I rG'GnC_Cy
Cuts at: 5737 6855 5737
Size: 1118 8657
EcoRI G'AATT_C
Cuts at: 2496 5197 7084 7462 2496
Size: 2701 1887 378 4809
Fragments arranged by size:
4809 2701 1887 378
EcoRV GAT'ATC
Cuts at: 2447 3241 4157 2447
Size: 794 916 8065
Fragments arranged by size:
8065 916 794
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6743 6743
Size: 9775
FspI TGC'GCA
Cuts at: 6835 8281 6835
Size: 1446 8329
HaeII r_GCGC'y
Cuts at: 1678 2233 3826 8922 1678
Size: 555 1593 5096 2531
Fragments arranged by size:
5096 2531 1593 555
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3225 3230 3257 3262 3688
Size: 32 135 32 1653 5 27 5 426
Cuts at: 3688 3720 4631 4663 5066 5098 5795 5827 6764
Size: 32 911 32 403 32 697 32 937
Cuts at: 6764 6796 8416 8448 8490 8522 1373
Size: 32 1620 32 42 32 2626
Fragments arranged by size:
2626 1653 1620 937 911 697 426 403
135 42 32 32 32 32 32 32
32 32 32 27 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2869 7119 7365 669
Size: 988 281 931 4250 246 3079
Fragments arranged by size:
4250 3079 988 931 281 246
HindIII A'AGCT_T
Cuts at: 748 2203 748
Size: 1455 8320
HpaI GTT'AAC
Cuts at: 2869 7365 2869
Size: 4496 5279
KasI G'GCGC_C
Cuts at: 3822 3822
Size: 9775
KpnI G_GTAC'C
Cuts at: 7100 7100
Size: 9775
MfeI C'AATT_G
Cuts at: 7374 7374
Size: 9775
MluI A'CGCG_T
Cuts at: 9651 9651
Size: 9775
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3311 4136 4519 4695 6337 8889 9073 1305
Size: 2006 825 383 176 1642 2552 184 2007
Fragments arranged by size:
2552 2007 2006 1642 825 383 184 176
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5825 6659 10
Size: 54 1425 343 3993 834 3126
Fragments arranged by size:
3993 3126 1425 834 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2633 2837 3381 4578 5158 5381 7733
Size: 648 1467 204 544 1197 580 223 2352
Cuts at: 7733 8092 8251 518
Size: 359 159 2042
Fragments arranged by size:
2352 2042 1467 1197 648 580 544 359
223 204 159
MspA1I CmG'CkG
Cuts at: 1606 4402 5565 5610 5915 7191 7868 9016 9261
Size: 2796 1163 45 305 1276 677 1148 245
Cuts at: 9261 1606
Size: 2120
Fragments arranged by size:
2796 2120 1276 1163 1148 677 305 245
45
NarI GG'CG_CC
Cuts at: 3823 3823
Size: 9775
NcoI C'CATG_G
Cuts at: 513 1065 2004 3903 5376 513
Size: 552 939 1899 1473 4912
Fragments arranged by size:
4912 1899 1473 939 552
NdeI CA'TA_TG
Cuts at: 387 2509 3153 6061 6398 387
Size: 2122 644 2908 337 3764
Fragments arranged by size:
3764 2908 2122 644 337
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9775
NotI GC'GGCC_GC
Cuts at: 7222 7222
Size: 9775
NsiI A_TGCA'T
Cuts at: 5430 5430
Size: 9775
NspI r_CATG'y
Cuts at: 1132 1229 2836 3349 4041 4053 4074 6175 6206
Size: 97 1607 513 692 12 21 2101 31
Cuts at: 6206 7133 8678 1132
Size: 927 1545 2229
Fragments arranged by size:
2229 2101 1607 1545 927 692 513 97
31 21 12
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9775
PciI A'CATG_T
Cuts at: 4049 4070 6171 8674 4049
Size: 21 2101 2503 5150
Fragments arranged by size:
5150 2503 2101 21
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2172 4445 4816 5851 1108
Size: 1064 2273 371 1035 5032
Fragments arranged by size:
5032 2273 1064 1035 371
PfoI T'CCnGG_A
Cuts at: 2346 3354 6366 2346
Size: 1008 3012 5755
Fragments arranged by size:
5755 3012 1008
PmeI GTTT'AAAC
Cuts at: 7079 7079
Size: 9775
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2487 7844 7876 9357 9389 2455
Size: 32 5357 32 1481 32 2841
Fragments arranged by size:
5357 2841 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9775
PsiI TTA'TAA
Cuts at: 7345 7345
Size: 9775
PspOMI G'GGCC_C
Cuts at: 2738 2738
Size: 9775
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3060 3092 3060
Size: 32 9743
PstI C_TGCA'G
Cuts at: 830 3538 5745 6715 7127 830
Size: 2708 2207 970 412 3478
Fragments arranged by size:
3478 2708 2207 970 412
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9775
PvuII CAG'CTG
Cuts at: 4402 5565 5610 5915 4402
Size: 1163 45 305 8262
Fragments arranged by size:
8262 1163 305 45
RsrII CG'GwC_CG
Cuts at: 5235 5235
Size: 9775
SacI G_AGCT'C
Cuts at: 4848 5541 4848
Size: 693 9082
SalI G'TCGA_C
Cuts at: 1936 7117 1936
Size: 5181 4594
SapI GCTCTTCn'nnn_
Cuts at: 6263 6263
Size: 9775
SbfI CC_TGCA'GG
Cuts at: 7127 7127
Size: 9775
ScaI AGT'ACT
Cuts at: 8023 9735 8023
Size: 1712 8063
SfcI C'TryA_G
Cuts at: 826 1045 3534 4838 4892 5528 5741 6711 7123
Size: 219 2489 1304 54 636 213 970 412
Cuts at: 7123 8258 8939 9130 826
Size: 1135 681 191 1471
Fragments arranged by size:
2489 1471 1304 1135 970 681 636 412
219 213 191 54
SfoI GGC'GCC
Cuts at: 3824 3824
Size: 9775
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9775
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9775
SmaI CCC'GGG
Cuts at: 1872 7102 1872
Size: 5230 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 3048 5007 5072 5189 5868
Size: 197 926 300 805 1959 65 117 679
Cuts at: 5868 6234 7879 8780 9042 820
Size: 366 1645 901 262 1553
Fragments arranged by size:
1959 1645 1553 926 901 805 679 366
300 262 197 117 65
SnaBI TAC'GTA
Cuts at: 493 7566 493
Size: 7073 2702
SpeI A'CTAG_T
Cuts at: 152 3169 152
Size: 3017 6758
SphI G_CATG'C
Cuts at: 1132 4041 6206 7133 1132
Size: 2909 2165 927 3774
Fragments arranged by size:
3774 2909 2165 927
SspI AAT'ATT
Cuts at: 5 52 7699 5
Size: 47 7647 2081
Fragments arranged by size:
7647 2081 47
StuI AGG'CCT
Cuts at: 1517 4487 1517
Size: 2970 6805
StyI C'CwwG_G
Cuts at: 513 1065 2004 2166 2742 3903 5376 6251 6576
Size: 552 939 162 576 1161 1473 875 325
Cuts at: 6576 7214 513
Size: 638 3074
Fragments arranged by size:
3074 1473 1161 939 875 638 576 552
325 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2531 3962 4918 5252 6438 6917 7802
Size: 1255 951 1431 956 334 1186 479 885
Cuts at: 7802 7819 7972 8157 8602 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1431 1255 1186 956 951 885 479
445 334 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3660 3764 3964
Size: 274 80 33 51 1443 1683 104 200
Cuts at: 3964 8021 9733 96
Size: 4057 1712 138
Fragments arranged by size:
4057 1712 1683 1443 274 200 138 104
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3258 3290 9735 9767 3258
Size: 32 6445 32 3266
Fragments arranged by size:
6445 3266 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2674 1316
Size: 1358 8417
XbaI T'CTAG_A
Cuts at: 7111 7111
Size: 9775
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2263 3637 4468 5461 2011
Size: 252 1374 831 993 6325
Fragments arranged by size:
6325 1374 993 831 252
XhoI C'TCGA_G
Cuts at: 1943 3048 5007 5868 1943
Size: 1105 1959 861 5850
Fragments arranged by size:
5850 1959 1105 861
XmaI C'CCGG_G
Cuts at: 1870 7100 1870
Size: 5230 4545
XmnI GAAnn'nnTTC
Cuts at: 2496 4008 4569 5034 6048 6138 7904 2496
Size: 1512 561 465 1014 90 1766 4367
Fragments arranged by size:
4367 1766 1512 1014 561 465 90
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3791 7583 276
Size: 53 83 186 936 2257 3792 2468
Fragments arranged by size:
3792 2468 2257 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NsiI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BsmBI EcoNI
FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI
PspXI SacII SanDI SexAI SfiI SrfI SwaI