Restriction map for
pFN21AB0001
(Circular) (Six-base) MAPSORT of: pFN21AB0001.seq Check: 6806 from: 1 to: 12238
pFN21AB0001 12238 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2645 3118 3445 5238 5323 5593 6683 7558 7813
Size: 473 327 1793 85 270 1090 875 255
Cuts at: 7813 8878 2645
Size: 1065 6005
Fragments arranged by size:
6005 1793 1090 1065 875 473 327 270
255 85
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7977 10048 278
Size: 53 83 186 936 6441 2071 2468
Fragments arranged by size:
6441 2468 2071 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6519 6702 6957 9581 1937
Size: 4582 183 255 2624 4594
Fragments arranged by size:
4594 4582 2624 255 183
Acc65I G'GTAC_C
Cuts at: 9559 9559
Size: 12238
AclI AA'CG_TT
Cuts at: 1563 10365 10738 1563
Size: 8802 373 3063
Fragments arranged by size:
8802 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2186 2437 2600 3620 4337 4349 4989 5495
Size: 554 251 163 1020 717 12 640 506
Cuts at: 5495 5552 5738 6197 6308 6353 7382 7619 9194
Size: 57 186 459 111 45 1029 237 1575
Cuts at: 9194 10301 11685 11953 1632
Size: 1107 1384 268 1917
Fragments arranged by size:
1917 1575 1384 1107 1029 1020 717 640
554 506 459 268 251 237 186 163
111 57 45 12
AfeI AGC'GCT
Cuts at: 3108 3902 3108
Size: 794 11444
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12041
AflIII A'CryG_T
Cuts at: 1203 6129 9517 11137 12114 1203
Size: 4926 3388 1620 977 1327
Fragments arranged by size:
4926 3388 1620 1327 977
AgeI A'CCGG_T
Cuts at: 11069 11069
Size: 12238
AhdI GACnn_n'nnGTC
Cuts at: 10967 10967
Size: 12238
AleI CACnn'nnGTG
Cuts at: 1166 2594 2897 5696 7097 8768 1166
Size: 1428 303 2799 1401 1671 4636
Fragments arranged by size:
4636 2799 1671 1428 1401 303
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2562 5895 7590 8348 9350 1184
Size: 1378 3333 1695 758 1002 4072
Fragments arranged by size:
4072 3333 1695 1378 1002 758
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8648 8680 8648
Size: 32 12206
AlwNI CAG_nnn'CTG
Cuts at: 2917 3145 3313 3346 3616 5200 5446 5473 5536
Size: 228 168 33 270 1584 246 27 63
Cuts at: 5536 5608 5842 5909 7714 8500 8572 9235 9250
Size: 72 234 67 1805 786 72 663 15
Cuts at: 9250 9981 11553 2917
Size: 731 1572 3602
Fragments arranged by size:
3602 1805 1584 1572 786 731 663 270
246 234 228 168 72 72 67 63
33 27 15
ApaI G_GGCC'C
Cuts at: 2463 3067 5506 7507 7861 7896 8690 8906 2463
Size: 604 2439 2001 354 35 794 216 5795
Fragments arranged by size:
5795 2439 2001 794 604 354 216 35
ApaLI G'TGCA_C
Cuts at: 3203 7486 8744 8821 10295 11451 3203
Size: 4283 1258 77 1474 1156 3990
Fragments arranged by size:
4283 3990 1474 1258 1156 77
ApoI r'AATT_y
Cuts at: 1493 7316 9547 9773 9925 12034 1493
Size: 5823 2231 226 152 2109 1697
Fragments arranged by size:
5823 2231 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10792 160
Size: 872 9760 1606
Fragments arranged by size:
9760 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12238
AvaI C'yCGr_G
Cuts at: 1870 1943 2375 2627 2931 3010 3910 3958 4157
Size: 73 432 252 304 79 900 48 199
Cuts at: 4157 4476 5053 5514 5969 6062 6355 6466 6615
Size: 319 577 461 455 93 293 111 149
Cuts at: 6615 6814 7004 7027 7033 7173 7860 8022 8826
Size: 199 190 23 6 140 687 162 804
Cuts at: 8826 8897 9563 1870
Size: 71 666 4545
Fragments arranged by size:
4545 900 804 687 666 577 461 455
432 319 304 293 252 199 199 190
162 149 140 111 93 79 73 71
48 23 6
BamHI G'GATC_C
Cuts at: 5896 7761 9568 9935 5896
Size: 1865 1807 367 8199
Fragments arranged by size:
8199 1865 1807 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2633 2753 2891 2994 3457
Size: 325 217 627 846 120 138 103 463
Cuts at: 3457 3857 3919 5335 5382 5485 6054 6683 6853
Size: 400 62 1416 47 103 569 629 170
Cuts at: 6853 6946 7803 7997 8186 9359 9559 11014 618
Size: 93 857 194 189 1173 200 1455 1842
Fragments arranged by size:
1842 1455 1416 1173 857 846 629 627
569 463 400 325 217 200 194 189
170 138 120 103 103 93 62 47
BanII G_rGCy'C
Cuts at: 1391 2136 2463 2938 3060 3067 3197 4098 4229
Size: 745 327 475 122 7 130 901 131
Cuts at: 4229 4950 5126 5295 5506 6396 6473 7034 7507
Size: 721 176 169 211 890 77 561 473
Cuts at: 7507 7861 7896 8514 8690 8723 8762 8906 8984
Size: 354 35 618 176 33 39 144 78
Cuts at: 8984 9557 1391
Size: 573 4072
Fragments arranged by size:
4072 901 890 745 721 618 573 561
475 473 354 327 211 176 176 169
144 131 130 122 78 77 39 35
33 7
BbeI G_GCGC'C
Cuts at: 3861 6687 7807 8190 9363 3861
Size: 2826 1120 383 1173 6736
Fragments arranged by size:
6736 2826 1173 1120 383
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4700 6315 7081 7900 8050 928
Size: 971 2801 1615 766 819 150 5116
Fragments arranged by size:
5116 2801 1615 971 819 766 150
BbvCI CC'TCA_GC
Cuts at: 2235 3719 3998 4576 5255 5983 6434 9087 2235
Size: 1484 279 578 679 728 451 2653 5386
Fragments arranged by size:
5386 2653 1484 728 679 578 451 279
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2629 2663 2922 2956 7144 7178 7397
Size: 34 692 34 259 34 4188 34 219
Cuts at: 7397 7431 7543 7577 8013 8047 8408 8442 10429
Size: 34 112 34 436 34 361 34 1987
Cuts at: 10429 10463 1903
Size: 34 3678
Fragments arranged by size:
4188 3678 1987 692 436 361 259 219
112 34 34 34 34 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9430 10130 11340 9430
Size: 700 1210 10328
Fragments arranged by size:
10328 1210 700
BclI T'GATC_A
Cuts at: 1548 9213 1548
Size: 7665 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2522 2631 2849
Size: 107 122 71 631 605 850 109 218
Cuts at: 2849 3912 5228 5846 6171 6328 6539 7880 8166
Size: 1063 1316 618 325 157 211 1341 286
Cuts at: 8166 8942 9231 10849 136
Size: 776 289 1618 1525
Fragments arranged by size:
1618 1525 1341 1316 1063 850 776 631
618 605 325 289 286 218 211 157
122 109 107 71
BglII A'GATC_T
Cuts at: 12233 12233
Size: 12238
BlpI GC'TnA_GC
Cuts at: 2621 3527 3620 3704 4142 5648 5783 5807 8795
Size: 906 93 84 438 1506 135 24 2988
Cuts at: 8795 9251 9294 9510 9655 2621
Size: 456 43 216 145 5204
Fragments arranged by size:
5204 2988 1506 906 456 438 216 145
135 93 84 43 24
Bme1580I G_kGCm'C
Cuts at: 1790 2463 2546 2636 2997 3067 3207 3462 3506
Size: 673 83 90 361 70 140 255 44
Cuts at: 3506 3924 5340 5490 5506 6057 7490 7507 7861
Size: 418 1416 150 16 551 1433 17 354
Cuts at: 7861 7896 8036 8690 8748 8825 8906 10299 11455
Size: 35 140 654 58 77 81 1393 1156
Cuts at: 11455 1790
Size: 2573
Fragments arranged by size:
2573 1433 1416 1393 1156 673 654 551
418 361 354 255 150 140 140 90
83 81 77 70 58 44 35 17
16
BmgBI CAC'GTC
Cuts at: 1318 4191 4534 5236 6451 8200 9430 1318
Size: 2873 343 702 1215 1749 1230 4126
Fragments arranged by size:
4126 2873 1749 1230 1215 702 343
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4159 4797 5089
Size: 416 53 83 399 24 2742 638 292
Cuts at: 5089 9969 10927 442
Size: 4880 958 1753
Fragments arranged by size:
4880 2742 1753 958 638 416 399 292
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3025 6958 8593 3025
Size: 3933 1635 6670
Fragments arranged by size:
6670 3933 1635
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2476 3068 3338 3838 4301 4403 4823 4838
Size: 1237 592 270 500 463 102 420 15
Cuts at: 4838 5051 5366 5612 5864 6133 6581 6950 7112
Size: 213 315 246 252 269 448 369 162
Cuts at: 7112 7718 7871 7964 8300 8522 9176 9479 9494
Size: 606 153 93 336 222 654 303 15
Cuts at: 9494 10898 1239
Size: 1404 2579
Fragments arranged by size:
2579 1404 1237 654 606 592 500 463
448 420 369 336 315 303 270 269
252 246 222 213 162 153 102 93
15 15
Bpu10I CC'TnA_GC
Cuts at: 2235 2363 3356 3719 3998 4263 4576 4679 5255
Size: 128 993 363 279 265 313 103 576
Cuts at: 5255 5983 6119 6434 8037 8321 9087 2235
Size: 728 136 315 1603 284 766 5386
Fragments arranged by size:
5386 1603 993 766 728 576 363 315
313 284 279 265 136 128 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2626 4082 5660 7466 7948 10363 11228 11526 2626
Size: 1456 1578 1806 482 2415 865 298 3338
Fragments arranged by size:
3338 2415 1806 1578 1456 865 482 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2342 2364 6495 10901 882
Size: 617 843 22 4131 4406 2219
Fragments arranged by size:
4406 4131 2219 843 617 22
BsaAI yAC'GTr
Cuts at: 493 1204 4258 9034 10029 493
Size: 711 3054 4776 995 2702
Fragments arranged by size:
4776 3054 2702 995 711
BsaBI GATnn'nnATC
Cuts at: 5114 12232 5114
Size: 7118 5120
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2322 3858 4431 4722
Size: 53 83 186 936 789 1536 573 291
Cuts at: 4722 5277 6684 6825 7518 7804 7905 7974 8187
Size: 555 1407 141 693 286 101 69 213
Cuts at: 8187 8270 9360 10045 10427 11062 275
Size: 83 1090 685 382 635 1451
Fragments arranged by size:
1536 1451 1407 1090 936 789 693 685
635 573 555 382 291 286 213 186
141 101 83 83 69 53
BsaWI w'CCGG_w
Cuts at: 6411 6892 7764 8556 10671 11069 11343 11490 11901
Size: 481 872 792 2115 398 274 147 411
Cuts at: 11901 6411
Size: 6748
Fragments arranged by size:
6748 2115 872 792 481 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2420 2450 3998 4028 4235
Size: 30 165 30 1017 30 1548 30 207
Cuts at: 4235 4265 4589 4619 5366 5396 6455 6485 6492
Size: 30 324 30 747 30 1059 30 7
Cuts at: 6492 6522 6614 6644 6695 6725 8538 8568 8648
Size: 30 92 30 51 30 1813 30 80
Cuts at: 8648 8678 9380 9410 1178
Size: 30 702 30 4006
Fragments arranged by size:
4006 1813 1548 1059 1017 747 702 324
207 165 92 80 51 30 30 30
30 30 30 30 30 30 30 30
30 30 30 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2479 3809 3902 5390 5426 6578 6593
Size: 838 455 1330 93 1488 36 1152 15
Cuts at: 6593 6640 6947 7595 8053 9200 1186
Size: 47 307 648 458 1147 4224
Fragments arranged by size:
4224 1488 1330 1152 1147 838 648 458
455 307 93 47 36 15
BseYI C'CCAG_C
Cuts at: 2462 2611 3103 3421 4468 5463 5711 5915 6273
Size: 149 492 318 1047 995 248 204 358
Cuts at: 6273 6639 6666 6687 7143 8259 8637 8961 8973
Size: 366 27 21 456 1116 378 324 12
Cuts at: 8973 8983 11441 11937 2462
Size: 10 2458 496 2763
Fragments arranged by size:
2763 2458 1116 1047 995 496 492 456
378 366 358 324 318 248 204 149
27 21 12 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2618 3059 3326 3470 4324 4397 4745
Size: 573 882 441 267 144 854 73 348
Cuts at: 4745 5459 6020 6679 7466 7586 7751 7841 8198
Size: 714 561 659 787 120 165 90 357
Cuts at: 8198 8735 8912 9140 1163
Size: 537 177 228 4261
Fragments arranged by size:
4261 882 854 787 714 659 573 561
537 441 357 348 267 228 177 165
144 120 90 73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2844 4542 4651 5827 7297 8241
Size: 275 186 843 1698 109 1176 1470 944
Cuts at: 8241 9688 10449 11477 1540
Size: 1447 761 1028 2301
Fragments arranged by size:
2301 1698 1470 1447 1176 1028 944 843
761 275 186 109
BsiHKAI G_wGCw'C
Cuts at: 3054 3207 3626 3638 4098 4269 6608 7490 8228
Size: 153 419 12 460 171 2339 882 738
Cuts at: 8228 8514 8723 8748 8825 8960 9144 10299 10384
Size: 286 209 25 77 135 184 1155 85
Cuts at: 10384 11455 3054
Size: 1071 3837
Fragments arranged by size:
3837 2339 1155 1071 882 738 460 419
286 209 184 171 153 135 85 77
25 12
BsmI GAATG_Cn'
Cuts at: 9749 9842 9749
Size: 93 12145
BsmBI CGTCTCn'nnnn_
Cuts at: 6816 7004 8210 9089 6816
Size: 188 1206 879 9965
Fragments arranged by size:
9965 1206 879 188
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2136 2463 2546 2636 2938 2997 3054
Size: 399 346 327 83 90 302 59 57
Cuts at: 3054 3060 3067 3197 3207 3462 3506 3626 3638
Size: 6 7 130 10 255 44 120 12
Cuts at: 3638 3924 4098 4229 4269 4950 5126 5295 5340
Size: 286 174 131 40 681 176 169 45
Cuts at: 5340 5490 5506 6057 6396 6473 6608 7034 7490
Size: 150 16 551 339 77 135 426 456
Cuts at: 7490 7507 7861 7896 8036 8228 8514 8690 8723
Size: 17 354 35 140 192 286 176 33
Cuts at: 8723 8748 8762 8825 8906 8960 8984 9144 9557
Size: 25 14 63 81 54 24 160 413
Cuts at: 9557 10299 10384 11455 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 681 551 456 426 413
399 354 346 339 327 302 286 286
255 192 176 176 174 169 160 150
140 135 131 130 120 90 85 83
81 77 63 59 57 54 45 44
40 35 33 25 24 17 16 14
12 10 7 6
BspEI T'CCGG_A
Cuts at: 7764 8556 11901 7764
Size: 792 3345 8101
Fragments arranged by size:
8101 3345 792
BspHI T'CATG_A
Cuts at: 8475 10127 11857 8475
Size: 1652 1730 8856
Fragments arranged by size:
8856 1730 1652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2645 3081 3118 3445 3708 5154 5238 5323
Size: 1801 436 37 327 263 1446 84 85
Cuts at: 5323 5592 5593 6683 7558 7777 7813 8667 8878
Size: 269 1 1090 875 219 36 854 211
Cuts at: 8878 9593 844
Size: 715 3489
Fragments arranged by size:
3489 1801 1446 1090 875 854 715 436
327 269 263 219 211 85 84 37
36 1
BsrBI CCG'CTC
Cuts at: 1394 1823 2340 2676 2826 3478 7342 8728 10125
Size: 429 517 336 150 652 3864 1386 1397
Cuts at: 10125 1394
Size: 3507
Fragments arranged by size:
3864 3507 1397 1386 652 517 429 336
150
BsrDI GCAATG_nn'
Cuts at: 65 1248 7869 8092 10733 10907 65
Size: 1183 6621 223 2641 174 1396
Fragments arranged by size:
6621 2641 1396 1183 223 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2024 2430 2750 4034 4397 4537 5744
Size: 110 214 406 320 1284 363 140 1207
Cuts at: 5744 6216 6250 7451 7794 8148 8918 10882 11069
Size: 472 34 1201 343 354 770 1964 187
Cuts at: 11069 1700
Size: 2869
Fragments arranged by size:
2869 1964 1284 1207 1201 770 472 406
363 354 343 320 214 187 140 110
34
BsrGI T'GTAC_A
Cuts at: 96 5202 96
Size: 5106 7132
BssHII G'CGCG_C
Cuts at: 1925 5395 9343 1925
Size: 3470 3948 4820
Fragments arranged by size:
4820 3948 3470
BssSI C'ACGA_G
Cuts at: 2384 7739 10298 11310 2384
Size: 5355 2559 1012 3312
Fragments arranged by size:
5355 3312 2559 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3313 3457 5335 5446 5536 5909 6043 7198
Size: 2519 144 1878 111 90 373 134 1155
Cuts at: 7198 794
Size: 5834
Fragments arranged by size:
5834 2519 1878 1155 373 144 134 111
90
BstBI TT'CG_AA
Cuts at: 11042 11042
Size: 12238
BstEII G'GTnAC_C
Cuts at: 1184 5458 5753 8017 9440 1184
Size: 4274 295 2264 1423 3982
Fragments arranged by size:
4274 3982 2264 1423 295
BstXI CCAn_nnnn'nTGG
Cuts at: 2500 5923 6777 6868 8602 2500
Size: 3423 854 91 1734 6136
Fragments arranged by size:
6136 3423 1734 854 91
BstYI r'GATC_y
Cuts at: 1972 3099 5508 5896 7761 9568 9935 10320 10337
Size: 1127 2409 388 1865 1807 367 385 17
Cuts at: 10337 11789 11875 11887 12233 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2409 1977 1865 1807 1452 1127 388 385
367 346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2797 3215 3297 3966 6576 2797
Size: 418 82 669 2610 8459
Fragments arranged by size:
8459 2610 669 418 82
BtgI C'CryG_G
Cuts at: 513 1065 2004 2881 3154 3406 3861 3870 4941
Size: 552 939 877 273 252 455 9 1071
Cuts at: 4941 5312 5697 5749 7098 7378 7498 7896 7920
Size: 371 385 52 1349 280 120 398 24
Cuts at: 7920 8124 8394 9204 9322 513
Size: 204 270 810 118 3429
Fragments arranged by size:
3429 1349 1071 939 877 810 552 455
398 385 371 280 273 270 252 204
120 118 52 24 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2432 2452 3358 4057 7042 7048
Size: 683 56 1206 20 906 699 2985 6
Cuts at: 7048 7654 487
Size: 606 5071
Fragments arranged by size:
5071 2985 1206 906 699 683 606 56
20 6
BtsI GCAGTG_nn'
Cuts at: 2546 4082 5207 5444 5691 6041 7420 7599 7715
Size: 1536 1125 237 247 350 1379 179 116
Cuts at: 7715 8186 8825 9754 10548 10568 2546
Size: 471 639 929 794 20 4216
Fragments arranged by size:
4216 1536 1379 1125 929 794 639 471
350 247 237 179 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 9337 9372 529
Size: 35 8773 35 3395
Fragments arranged by size:
8773 3395 35 35
DraI TTT'AAA
Cuts at: 9542 9542
Size: 12238
DraIII CAC_nnn'GTG
Cuts at: 3510 4273 3510
Size: 763 11475
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2710 4652 8240 11245 809
Size: 507 1394 1942 3588 3005 1802
Fragments arranged by size:
3588 3005 1942 1802 1394 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2424 2691 2841
Size: 54 1425 50 275 18 594 267 150
Cuts at: 2841 3027 3739 4395 4539 5127 5268 5742 5746
Size: 186 712 656 144 588 141 474 4
Cuts at: 5746 5817 6106 6738 6870 7626 7917 8172 8916
Size: 71 289 632 132 756 291 255 744
Cuts at: 8916 9685 10574 12203 8
Size: 769 889 1629 43
Fragments arranged by size:
1629 1425 889 769 756 744 712 656
632 594 588 474 291 289 275 267
255 186 150 144 141 132 71 54
50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 2841 4539 9685 1537
Size: 275 1029 1698 5146 4090
Fragments arranged by size:
5146 4090 1698 1029 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4664 4913 4958 6149 6209 6319 7248 8300
Size: 3311 249 45 1191 60 110 929 1052
Cuts at: 8300 9011 10168 1353
Size: 711 1157 3423
Fragments arranged by size:
3423 3311 1191 1157 1052 929 711 249
110 60 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4186 6374 6536 7265 7781 8110
Size: 1064 138 2597 2188 162 729 516 329
Cuts at: 8110 8468 8600 10825 11199 11345 387
Size: 358 132 2225 374 146 1280
Fragments arranged by size:
2597 2225 2188 1280 1064 729 516 374
358 329 162 146 138 132
EcoICRI GAG'CTC
Cuts at: 4096 8512 8721 4096
Size: 4416 209 7613
Fragments arranged by size:
7613 4416 209
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2186 2437 2476 2600 3068 3338 3620
Size: 393 554 251 39 124 468 270 282
Cuts at: 3620 3838 4301 4337 4349 4403 4823 4838 4989
Size: 218 463 36 12 54 420 15 151
Cuts at: 4989 5051 5366 5495 5552 5612 5738 5864 6133
Size: 62 315 129 57 60 126 126 269
Cuts at: 6133 6197 6308 6353 6581 6950 7112 7382 7619
Size: 64 111 45 228 369 162 270 237
Cuts at: 7619 7718 7871 7964 8300 8522 9176 9194 9479
Size: 99 153 93 336 222 654 18 285
Cuts at: 9479 9494 10301 10898 11685 11953 1239
Size: 15 807 597 787 268 1524
Fragments arranged by size:
1524 807 787 654 597 554 468 463
420 393 369 336 315 285 282 270
270 269 268 251 237 228 222 218
162 153 151 129 126 126 124 111
99 93 64 62 60 57 54 45
39 36 18 15 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3029 3752 3788 3817 4304 4961 5408 6122 6560
Size: 723 36 29 487 657 447 714 438
Cuts at: 6560 8387 9398 3029
Size: 1827 1011 5869
Fragments arranged by size:
5869 1827 1011 723 714 657 487 447
438 36 29
EcoO109I rG'GnC_Cy
Cuts at: 2170 2359 2459 3064 3325 3343 3962 3970 4246
Size: 189 100 605 261 18 619 8 276
Cuts at: 4246 5502 5518 5638 5839 5974 6531 6622 6865
Size: 1256 16 120 201 135 557 91 243
Cuts at: 6865 7384 7503 7857 7864 8380 8686 8687 8902
Size: 519 119 354 7 516 306 1 215
Cuts at: 8902 8903 8934 9178 9232 9373 2170
Size: 1 31 244 54 141 5035
Fragments arranged by size:
5035 1256 619 605 557 519 516 354
306 276 261 244 243 215 201 189
141 135 120 119 100 91 54 31
18 16 8 7 1 1
EcoRI G'AATT_C
Cuts at: 9547 9925 9547
Size: 378 11860
EcoRV GAT'ATC
Cuts at: 2806 2806
Size: 12238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5374 6202 5374
Size: 828 11410
FseI GG_CCGG'CC
Cuts at: 5748 5748
Size: 12238
FspI TGC'GCA
Cuts at: 10744 10744
Size: 12238
HaeII r_GCGC'y
Cuts at: 1678 3110 3475 3861 3904 5922 5934 6687 7442
Size: 1432 365 386 43 2018 12 753 755
Cuts at: 7442 7807 8190 9164 9363 11385 1678
Size: 365 383 974 199 2022 2531
Fragments arranged by size:
2531 2022 2018 1432 974 755 753 386
383 365 365 199 43 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2218 2250 3546 3578 3816
Size: 32 135 32 646 32 1296 32 238
Cuts at: 3816 3848 4589 4621 5966 5998 6296 6328 6455
Size: 32 741 32 1345 32 298 32 127
Cuts at: 6455 6487 6614 6646 10879 10911 10953 10985 1373
Size: 32 127 32 4233 32 42 32 2626
Fragments arranged by size:
4233 2626 1345 1296 741 646 298 238
135 127 127 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4213 7228 7746 8434 9582 9828
Size: 988 281 2275 3015 518 688 1148 246
Cuts at: 9828 669
Size: 3079
Fragments arranged by size:
3079 3015 2275 1148 988 688 518 281
246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 12238
HpaI GTT'AAC
Cuts at: 9828 9828
Size: 12238
KasI G'GCGC_C
Cuts at: 3857 6683 7803 8186 9359 3857
Size: 2826 1120 383 1173 6736
Fragments arranged by size:
6736 2826 1173 1120 383
KpnI G_GTAC'C
Cuts at: 9563 9563
Size: 12238
MfeI C'AATT_G
Cuts at: 9837 9837
Size: 12238
MluI A'CGCG_T
Cuts at: 6129 9517 12114 6129
Size: 3388 2597 6253
Fragments arranged by size:
6253 3388 2597
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2031 3009 3093 3222 5370 6453 6942 11352
Size: 726 978 84 129 2148 1083 489 4410
Cuts at: 11352 11536 1305
Size: 184 2007
Fragments arranged by size:
4410 2148 2007 1083 978 726 489 184
129 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2426 3029 6108 6740 6872
Size: 54 1425 343 594 603 3079 632 132
Cuts at: 6872 10
Size: 5376
Fragments arranged by size:
5376 3079 1425 632 603 594 343 132
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2594 2897 3395 5696 7097 8210 8480
Size: 648 1428 303 498 2301 1401 1113 270
Cuts at: 8480 8696 8768 9134 10196 10555 10714 518
Size: 216 72 366 1062 359 159 2042
Fragments arranged by size:
2301 2042 1428 1401 1113 1062 648 498
366 359 303 270 216 159 72
MspA1I CmG'CkG
Cuts at: 1606 2239 2535 2657 2962 3285 3464 3613 3997
Size: 633 296 122 305 323 179 149 384
Cuts at: 3997 4137 4732 5314 5590 5652 5806 6273 6639
Size: 140 595 582 276 62 154 467 366
Cuts at: 6639 6831 7995 8377 8653 8965 9172 9293 9468
Size: 192 1164 382 276 312 207 121 175
Cuts at: 9468 9654 10331 11479 11724 1606
Size: 186 677 1148 245 2120
Fragments arranged by size:
2120 1164 1148 677 633 595 582 467
384 382 366 323 312 305 296 276
276 245 207 192 186 179 175 154
149 140 122 121 62
NaeI GCC'GGC
Cuts at: 2026 2752 4399 4539 5746 6218 7453 8150 8920
Size: 726 1647 140 1207 472 1235 697 770
Cuts at: 8920 2026
Size: 5344
Fragments arranged by size:
5344 1647 1235 1207 770 726 697 472
140
NarI GG'CG_CC
Cuts at: 3858 6684 7804 8187 9360 3858
Size: 2826 1120 383 1173 6736
Fragments arranged by size:
6736 2826 1173 1120 383
NcoI C'CATG_G
Cuts at: 513 1065 2004 3861 7498 8124 9322 513
Size: 552 939 1857 3637 626 1198 3429
Fragments arranged by size:
3637 3429 1857 1198 939 626 552
NdeI CA'TA_TG
Cuts at: 387 9107 387
Size: 8720 3518
NgoMIV G'CCGG_C
Cuts at: 2024 2750 4397 4537 5744 6216 7451 8148 8918
Size: 726 1647 140 1207 472 1235 697 770
Cuts at: 8918 2024
Size: 5344
Fragments arranged by size:
5344 1647 1235 1207 770 726 697 472
140
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12238
NotI GC'GGCC_GC
Cuts at: 9685 9685
Size: 12238
NspI r_CATG'y
Cuts at: 1132 1229 2032 3257 3364 5710 6596 7199 8610
Size: 97 803 1225 107 2346 886 603 1411
Cuts at: 8610 9596 11141 1132
Size: 986 1545 2229
Fragments arranged by size:
2346 2229 1545 1411 1225 986 886 803
603 107 97
PasI CC'CwG_GG
Cuts at: 1348 3748 4711 5884 5923 6770 1348
Size: 2400 963 1173 39 847 6816
Fragments arranged by size:
6816 2400 1173 963 847 39
PciI A'CATG_T
Cuts at: 11137 11137
Size: 12238
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3346 3748 4522 4627 5842 6639 8266 8500
Size: 2238 402 774 105 1215 797 1627 234
Cuts at: 8500 8782 8857 9235 1108
Size: 282 75 378 4111
Fragments arranged by size:
4111 2238 1627 1215 797 774 402 378
282 234 105 75
PfoI T'CCnGG_A
Cuts at: 2033 2808 4110 4239 4815 5028 5343 5658 6453
Size: 775 1302 129 576 213 315 315 795
Cuts at: 6453 7302 7458 7757 7968 2033
Size: 849 156 299 211 6303
Fragments arranged by size:
6303 1302 849 795 775 576 315 315
299 213 211 156 129
PmeI GTTT'AAAC
Cuts at: 9542 9542
Size: 12238
PmlI CAC'GTG
Cuts at: 4258 4258
Size: 12238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8648 8680 10307 10339 11820 11852 8648
Size: 32 1627 32 1481 32 9034
Fragments arranged by size:
9034 1627 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2170 3343 3962 5518 5638 5974 6622 7384 8380
Size: 1173 619 1556 120 336 648 762 996
Cuts at: 8380 9373 2170
Size: 993 5035
Fragments arranged by size:
5035 1556 1173 996 993 762 648 619
336 120
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12238
PsiI TTA'TAA
Cuts at: 9808 9808
Size: 12238
PspOMI G'GGCC_C
Cuts at: 2459 3063 5502 7503 7857 7892 8686 8902 2459
Size: 604 2439 2001 354 35 794 216 5795
Fragments arranged by size:
5795 2439 2001 794 604 354 216 35
PstI C_TGCA'G
Cuts at: 830 2511 3129 4092 4308 5109 5214 5409 5451
Size: 1681 618 963 216 801 105 195 42
Cuts at: 5451 5564 5589 5805 5868 6486 7266 8568 8820
Size: 113 25 216 63 618 780 1302 252
Cuts at: 8820 8970 9590 830
Size: 150 620 3478
Fragments arranged by size:
3478 1681 1302 963 801 780 620 618
618 252 216 216 195 150 113 105
63 42 25
PvuI CG_AT'CG
Cuts at: 2001 8241 2001
Size: 6240 5998
PvuII CAG'CTG
Cuts at: 2239 3613 3997 4732 5590 5806 8653 8965 2239
Size: 1374 384 735 858 216 2847 312 5512
Fragments arranged by size:
5512 2847 1374 858 735 384 312 216
RsrII CG'GwC_CG
Cuts at: 2154 4032 2154
Size: 1878 10360
SacI G_AGCT'C
Cuts at: 4098 8514 8723 4098
Size: 4416 209 7613
Fragments arranged by size:
7613 4416 209
SacII CC_GC'GG
Cuts at: 5315 5315
Size: 12238
SalI G'TCGA_C
Cuts at: 1936 9580 1936
Size: 7644 4594
SanDI GG'GwC_CC
Cuts at: 5518 5974 5518
Size: 456 11782
SapI GCTCTTCn'nnn_
Cuts at: 4913 6209 4913
Size: 1296 10942
SbfI CC_TGCA'GG
Cuts at: 5409 5564 9590 5409
Size: 155 4026 8057
Fragments arranged by size:
8057 4026 155
ScaI AGT'ACT
Cuts at: 10486 12198 10486
Size: 1712 10526
SexAI A'CCwGG_T
Cuts at: 4218 4626 4857 6504 7230 8382 8517 4218
Size: 408 231 1647 726 1152 135 7939
Fragments arranged by size:
7939 1647 1152 726 408 231 135
SfcI C'TryA_G
Cuts at: 826 1045 2392 2507 3125 4088 4304 5105 5210
Size: 219 1347 115 618 963 216 801 105
Cuts at: 5210 5405 5447 5560 5585 5801 5864 6482 6958
Size: 195 42 113 25 216 63 618 476
Cuts at: 6958 7262 8356 8564 8816 8966 9586 10721 11402
Size: 304 1094 208 252 150 620 1135 681
Cuts at: 11402 11593 826
Size: 191 1471
Fragments arranged by size:
1471 1347 1135 1094 963 801 681 620
618 618 476 304 252 219 216 216
208 195 191 150 115 113 105 63
42 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 2849 8166 8942 2849
Size: 5317 776 6145
Fragments arranged by size:
6145 5317 776
SfoI GGC'GCC
Cuts at: 3859 6685 7805 8188 9361 3859
Size: 2826 1120 383 1173 6736
Fragments arranged by size:
6736 2826 1173 1120 383
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12238
SgrAI Cr'CCGG_yG
Cuts at: 1700 7794 1700
Size: 6094 6144
SmaI CCC'GGG
Cuts at: 1872 3912 5516 6468 6617 7006 7175 7862 9565
Size: 2040 1604 952 149 389 169 687 1703
Cuts at: 9565 1872
Size: 4545
Fragments arranged by size:
4545 2040 1703 1604 952 687 389 169
149
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2605 4097 5675 7481 7927 10342
Size: 197 926 662 1492 1578 1806 446 2415
Cuts at: 10342 11243 11505 820
Size: 901 262 1553
Fragments arranged by size:
2415 1806 1578 1553 1492 926 901 662
446 262 197
SnaBI TAC'GTA
Cuts at: 493 9034 10029 493
Size: 8541 995 2702
Fragments arranged by size:
8541 2702 995
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12238
SphI G_CATG'C
Cuts at: 1132 3364 8610 9596 1132
Size: 2232 5246 986 3774
Fragments arranged by size:
5246 3774 2232 986
SspI AAT'ATT
Cuts at: 5 52 10162 5
Size: 47 10110 2081
Fragments arranged by size:
10110 2081 47
StuI AGG'CCT
Cuts at: 1517 2645 3690 4823 5681 5778 8160 8169 1517
Size: 1128 1045 1133 858 97 2382 9 5586
Fragments arranged by size:
5586 2382 1133 1128 1045 858 97 9
StyI C'CwwG_G
Cuts at: 513 1065 2004 2895 3030 3861 3939 4020 5454
Size: 552 939 891 135 831 78 81 1434
Cuts at: 5454 6861 7245 7498 7537 8124 8619 9309 9322
Size: 1407 384 253 39 587 495 690 13
Cuts at: 9322 9677 513
Size: 355 3074
Fragments arranged by size:
3074 1434 1407 939 891 831 690 587
552 495 384 355 253 135 81 78
39 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 3413 5071 5201 6265 7673 8763
Size: 1255 737 1096 1658 130 1064 1408 1090
Cuts at: 8763 9008 9062 9322 10265 10282 10435 10620 11065
Size: 245 54 260 943 17 153 185 445
Cuts at: 11065 325
Size: 1498
Fragments arranged by size:
1658 1498 1408 1255 1096 1090 1064 943
737 445 260 245 185 153 130 54
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4203 5202 7989
Size: 274 80 33 51 1443 2226 999 2787
Cuts at: 7989 9300 10484 12196 96
Size: 1311 1184 1712 138
Fragments arranged by size:
2787 2226 1712 1443 1311 1184 999 274
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3994 4026 4235 4267 5366 5398 5747 5779 6492
Size: 32 209 32 1099 32 349 32 713
Cuts at: 6492 6524 6882 6914 12198 12230 3994
Size: 32 358 32 5284 32 4002
Fragments arranged by size:
5284 4002 1099 713 358 349 209 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2099 3650 3947 4795 7232 1316
Size: 783 1551 297 848 2437 6322
Fragments arranged by size:
6322 2437 1551 848 783 297
XbaI T'CTAG_A
Cuts at: 9574 9574
Size: 12238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2470 2713 2918 2986 3868 4506 6358 6925
Size: 459 243 205 68 882 638 1852 567
Cuts at: 6925 8122 8197 9034 2011
Size: 1197 75 837 5215
Fragments arranged by size:
5215 1852 1197 882 837 638 567 459
243 205 75 68
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12238
XmaI C'CCGG_G
Cuts at: 1870 3910 5514 6466 6615 7004 7173 7860 9563
Size: 2040 1604 952 149 389 169 687 1703
Cuts at: 9563 1870
Size: 4545
Fragments arranged by size:
4545 2040 1703 1604 952 687 389 169
149
XmnI GAAnn'nnTTC
Cuts at: 3590 6185 10367 3590
Size: 2595 4182 5461
Fragments arranged by size:
5461 4182 2595
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7975 10046 276
Size: 53 83 186 936 6441 2071 2468
Fragments arranged by size:
6441 2468 2071 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I FspAI NruI
NsiI PacI PspXI PsrI SrfI SwaI