Restriction map for
pFN21AA2033
(Circular) (Six-base) MAPSORT of: pFN21AA2033.seq Check: 1722 from: 1 to: 6943
pFN21AA2033 6943 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2852 3382 3860 4753
Size: 53 83 186 936 1316 530 478 893
Cuts at: 4753 278
Size: 2468
Fragments arranged by size:
2468 1316 936 893 530 478 186 83
53
AccI GT'mk_AC
Cuts at: 1937 2872 3208 3376 3628 3712 3880 4216 4286
Size: 935 336 168 252 84 168 336 70
Cuts at: 4286 1937
Size: 4594
Fragments arranged by size:
4594 935 336 336 252 168 168 84
70
Acc65I G'GTAC_C
Cuts at: 4264 4264
Size: 6943
AclI AA'CG_TT
Cuts at: 1563 5070 5443 1563
Size: 3507 373 3063
Fragments arranged by size:
3507 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2824 2988 3324 3832 5006 6390 6658 1632
Size: 1192 164 336 508 1174 1384 268 1917
Fragments arranged by size:
1917 1384 1192 1174 508 336 268 164
AflII C'TTAA_G
Cuts at: 820 1017 2692 820
Size: 197 1675 5071
Fragments arranged by size:
5071 1675 197
AflIII A'CryG_T
Cuts at: 1203 5842 6819 1203
Size: 4639 977 1327
Fragments arranged by size:
4639 1327 977
AgeI A'CCGG_T
Cuts at: 5774 5774
Size: 6943
AhdI GACnn_n'nnGTC
Cuts at: 2841 3093 3177 3513 3849 4017 4185 5672 2841
Size: 252 84 336 336 168 168 1487 4112
Fragments arranged by size:
4112 1487 336 336 252 168 168 84
AleI CACnn'nnGTG
Cuts at: 1166 2799 1166
Size: 1633 5310
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6943
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2123 2155 2123
Size: 32 6911
AlwNI CAG_nnn'CTG
Cuts at: 4686 6258 4686
Size: 1572 5371
ApaLI G'TGCA_C
Cuts at: 5000 6156 5000
Size: 1156 5787
ApoI r'AATT_y
Cuts at: 1493 2051 2297 2541 2596 3274 3294 3462 3798
Size: 558 246 244 55 678 20 168 336
Cuts at: 3798 3966 4252 4478 4630 6739 1493
Size: 168 286 226 152 2109 1697
Fragments arranged by size:
2109 1697 678 558 336 286 246 244
226 168 168 152 55 20
AseI AT'TA_AT
Cuts at: 160 1032 5497 160
Size: 872 4465 1606
Fragments arranged by size:
4465 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
AvaI C'yCGr_G
Cuts at: 1870 1943 4268 1870
Size: 73 2325 4545
Fragments arranged by size:
4545 2325 73
BamHI G'GATC_C
Cuts at: 4273 4640 4273
Size: 367 6576
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4264 5719 618
Size: 325 217 627 2477 1455 1842
Fragments arranged by size:
2477 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4262 1391
Size: 2871 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3312 928
Size: 971 1413 4559
Fragments arranged by size:
4559 1413 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5134 5168 1903
Size: 34 3197 34 3678
Fragments arranged by size:
3678 3197 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4835 6045 4835
Size: 1210 5733
BclI T'GATC_A
Cuts at: 1548 2383 1548
Size: 835 6108
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5554 136
Size: 107 122 71 631 605 3882 1525
Fragments arranged by size:
3882 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6938 6938
Size: 6943
BlpI GC'TnA_GC
Cuts at: 4360 4360
Size: 6943
Bme1580I G_kGCm'C
Cuts at: 1790 5004 6160 1790
Size: 3214 1156 2573
Fragments arranged by size:
3214 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2110 1318
Size: 792 6151
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4674 5632 442
Size: 416 53 83 399 24 3257 958 1753
Fragments arranged by size:
3257 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2904 3072 3156 3240 3408 3492 3576
Size: 899 766 168 84 84 168 84 84
Cuts at: 3576 3660 3996 4080 4164 4248 5603 1239
Size: 84 336 84 84 84 1355 2579
Fragments arranged by size:
2579 1355 899 766 336 168 168 84
84 84 84 84 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5068 5933 6231 5068
Size: 865 298 5780
Fragments arranged by size:
5780 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2143 5606 882
Size: 617 644 3463 2219
Fragments arranged by size:
3463 2219 644 617
BsaAI yAC'GTr
Cuts at: 493 1204 4734 493
Size: 711 3530 2702
Fragments arranged by size:
3530 2702 711
BsaBI GATnn'nnATC
Cuts at: 6937 6937
Size: 6943
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2849 3379 3857 4750
Size: 53 83 186 936 1316 530 478 893
Cuts at: 4750 5132 5767 275
Size: 382 635 1451
Fragments arranged by size:
1451 1316 936 893 635 530 478 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3890 5376 5774 6048 6195 6606 3890
Size: 1486 398 274 147 411 4227
Fragments arranged by size:
4227 1486 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2123 2153 2165 2195 1178
Size: 30 165 30 720 30 12 30 5926
Fragments arranged by size:
5926 720 165 30 30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 6051
BseYI C'CCAG_C
Cuts at: 6146 6642 6146
Size: 496 6447
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3604 1163
Size: 573 1868 4502
Fragments arranged by size:
4502 1868 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4393 5154 6182 1540
Size: 275 186 2392 761 1028 2301
Fragments arranged by size:
2392 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5004 5089 6160 5004
Size: 85 1071 5787
Fragments arranged by size:
5787 1071 85
BsmI GAATG_Cn'
Cuts at: 4454 4547 4454
Size: 93 6850
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4262 5004 5089 6160 1391
Size: 399 2472 742 85 1071 2174
Fragments arranged by size:
2472 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6606 6606
Size: 6943
BspHI T'CATG_A
Cuts at: 2287 4832 6562 2287
Size: 2545 1730 2668
Fragments arranged by size:
2668 2545 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3626 4298 844
Size: 2782 672 3489
Fragments arranged by size:
3489 2782 672
BsrBI CCG'CTC
Cuts at: 1394 1823 4830 1394
Size: 429 3007 3507
Fragments arranged by size:
3507 3007 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2222 5438 5612 65
Size: 1183 974 3216 174 1396
Fragments arranged by size:
3216 1396 1183 974 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5587 5774 1700
Size: 110 3777 187 2869
Fragments arranged by size:
3777 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6943
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6943
BssSI C'ACGA_G
Cuts at: 5003 6015 5003
Size: 1012 5931
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6943
BstBI TT'CG_AA
Cuts at: 5747 5747
Size: 6943
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6943
BstYI r'GATC_y
Cuts at: 1972 4273 4640 5025 5042 6494 6580 6592 6938
Size: 2301 367 385 17 1452 86 12 346
Cuts at: 6938 1972
Size: 1977
Fragments arranged by size:
2301 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2578 513
Size: 552 939 574 4878
Fragments arranged by size:
4878 939 574 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6204
Fragments arranged by size:
6204 683 56
BtsI GCAGTG_nn'
Cuts at: 4459 5253 5273 4459
Size: 794 20 6129
Fragments arranged by size:
6129 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6943
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6908
DraI TTT'AAA
Cuts at: 4247 4247
Size: 6943
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5950 809
Size: 507 4634 1802
Fragments arranged by size:
4634 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2326 4390
Size: 54 1425 50 275 18 213 283 2064
Cuts at: 4390 5279 6908 8
Size: 889 1629 43
Fragments arranged by size:
2064 1629 1425 889 283 275 213 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4390 1537
Size: 275 2578 4090
Fragments arranged by size:
4090 2578 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2158 2469 2964 4873 1353
Size: 805 311 495 1909 3423
Fragments arranged by size:
3423 1909 805 495 311
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5530 5904 6050 387
Size: 1064 138 3941 374 146 1280
Fragments arranged by size:
3941 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2824 2904 2988 3072 3156 3240
Size: 393 506 686 80 84 84 84 84
Cuts at: 3240 3324 3408 3492 3576 3660 3832 3996 4080
Size: 84 84 84 84 84 172 164 84
Cuts at: 4080 4164 4248 5006 5603 6390 6658 1239
Size: 84 84 758 597 787 268 1524
Fragments arranged by size:
1524 787 758 686 597 506 393 268
172 164 84 84 84 84 84 84
84 84 84 84 84 84 80
EcoRI G'AATT_C
Cuts at: 2051 2596 3462 3966 4252 4630 2051
Size: 545 866 504 286 378 4364
Fragments arranged by size:
4364 866 545 504 378 286
EcoRV GAT'ATC
Cuts at: 2149 2149
Size: 6943
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2194 2438 2562 2194
Size: 244 124 6575
Fragments arranged by size:
6575 244 124
FspI TGC'GCA
Cuts at: 5449 5449
Size: 6943
HaeII r_GCGC'y
Cuts at: 1678 6090 1678
Size: 4412 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2165 2197 2827 2859 3079
Size: 32 135 32 593 32 630 32 220
Cuts at: 3079 3111 3163 3195 3499 3531 3667 3699 3835
Size: 32 52 32 304 32 136 32 136
Cuts at: 3835 3867 4003 4035 4171 4203 5584 5616 5658
Size: 32 136 32 136 32 1381 32 42
Cuts at: 5658 5690 1373
Size: 32 2626
Fragments arranged by size:
2626 1381 630 593 304 220 136 136
136 136 135 52 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2184 2361 3693 4287 4533 669
Size: 988 281 246 177 1332 594 246 3079
Fragments arranged by size:
3079 1332 988 594 281 246 246 177
HindIII A'AGCT_T
Cuts at: 748 2431 2919 3255 3423 3564 3759 3927 748
Size: 1683 488 336 168 141 195 168 3764
Fragments arranged by size:
3764 1683 488 336 195 168 168 141
HpaI GTT'AAC
Cuts at: 3693 4533 3693
Size: 840 6103
KpnI G_GTAC'C
Cuts at: 4268 4268
Size: 6943
MfeI C'AATT_G
Cuts at: 4542 4542
Size: 6943
MluI A'CGCG_T
Cuts at: 6819 6819
Size: 6943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3497 3581 3833 4001 6057 6241 1305
Size: 2192 84 252 168 2056 184 2007
Fragments arranged by size:
2192 2056 2007 252 184 168 84
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 2328 10
Size: 54 1425 343 213 283 4625
Fragments arranged by size:
4625 1425 343 283 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2247 2292 2799 2983 3487 4901 5260
Size: 648 1081 45 507 184 504 1414 359
Cuts at: 5260 5419 518
Size: 159 2042
Fragments arranged by size:
2042 1414 1081 648 507 504 359 184
159 45
MspA1I CmG'CkG
Cuts at: 1606 4359 5036 6184 6429 1606
Size: 2753 677 1148 245 2120
Fragments arranged by size:
2753 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2578 513
Size: 552 939 574 4878
Fragments arranged by size:
4878 939 574 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6943
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6943
NotI GC'GGCC_GC
Cuts at: 4390 4390
Size: 6943
NspI r_CATG'y
Cuts at: 1132 1229 2781 2865 3117 3201 3369 3537 3873
Size: 97 1552 84 252 84 168 168 336
Cuts at: 3873 4209 4301 5846 1132
Size: 336 92 1545 2229
Fragments arranged by size:
2229 1552 1545 336 336 252 168 168
97 92 84 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6943
PciI A'CATG_T
Cuts at: 5842 5842
Size: 6943
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6943
PmeI GTTT'AAAC
Cuts at: 4247 4247
Size: 6943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2123 2155 5012 5044 6525 6557 2123
Size: 32 2857 32 1481 32 2509
Fragments arranged by size:
2857 2509 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6943
PsiI TTA'TAA
Cuts at: 4074 4513 4074
Size: 439 6504
PstI C_TGCA'G
Cuts at: 830 4295 830
Size: 3465 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6943
SalI G'TCGA_C
Cuts at: 1936 4285 1936
Size: 2349 4594
SbfI CC_TGCA'GG
Cuts at: 4295 4295
Size: 6943
ScaI AGT'ACT
Cuts at: 5191 6903 5191
Size: 1712 5231
SexAI A'CCwGG_T
Cuts at: 2133 2133
Size: 6943
SfcI C'TryA_G
Cuts at: 826 1045 4291 5426 6107 6298 826
Size: 219 3246 1135 681 191 1471
Fragments arranged by size:
3246 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6943
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6943
SmaI CCC'GGG
Cuts at: 1872 4270 1872
Size: 2398 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2692 5047 5948 6210 820
Size: 197 926 749 2355 901 262 1553
Fragments arranged by size:
2355 1553 926 901 749 262 197
SnaBI TAC'GTA
Cuts at: 493 4734 493
Size: 4241 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6943
SphI G_CATG'C
Cuts at: 1132 2781 4301 1132
Size: 1649 1520 3774
Fragments arranged by size:
3774 1649 1520
SspI AAT'ATT
Cuts at: 5 52 4867 5
Size: 47 4815 2081
Fragments arranged by size:
4815 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6943
StyI C'CwwG_G
Cuts at: 513 1065 2004 2520 2578 4382 513
Size: 552 939 516 58 1804 3074
Fragments arranged by size:
3074 1804 939 552 516 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2353 2486 4970 4987 5140 5325 5770
Size: 1255 773 133 2484 17 153 185 445
Cuts at: 5770 325
Size: 1498
Fragments arranged by size:
2484 1498 1255 773 445 185 153 133
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2369 2626 5189
Size: 274 80 33 51 1443 392 257 2563
Cuts at: 5189 6901 96
Size: 1712 138
Fragments arranged by size:
2563 1712 1443 392 274 257 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 3089 3121 3173 3205 6903 6935 2501
Size: 32 556 32 52 32 3698 32 2509
Fragments arranged by size:
3698 2509 556 52 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6943
XbaI T'CTAG_A
Cuts at: 4279 4279
Size: 6943
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6943
XmaI C'CCGG_G
Cuts at: 1870 4268 1870
Size: 2398 4545
XmnI GAAnn'nnTTC
Cuts at: 2174 2588 5072 2174
Size: 414 2484 4045
Fragments arranged by size:
4045 2484 414
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2850 3380 3858 4751
Size: 53 83 186 936 1316 530 478 893
Cuts at: 4751 276
Size: 2468
Fragments arranged by size:
2468 1316 936 893 530 478 186 83
53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SfiI SfoI SrfI SwaI XcmI