Restriction map for
pFN21AA2027
(Circular) (Six-base) MAPSORT of: pFN21AA2027.seq Check: 6590 from: 1 to: 7033
pFN21AA2027 7033 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2284 2414 2284
Size: 130 6903
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4056 4843 278
Size: 53 83 186 936 2520 787 2468
Fragments arranged by size:
2520 2468 936 787 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3206 3656 4376 1937
Size: 1269 450 720 4594
Fragments arranged by size:
4594 1269 720 450
Acc65I G'GTAC_C
Cuts at: 4354 4354
Size: 7033
AclI AA'CG_TT
Cuts at: 1563 5160 5533 1563
Size: 3597 373 3063
Fragments arranged by size:
3597 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2478 2682 3056 5096 6480 6748 1632
Size: 846 204 374 2040 1384 268 1917
Fragments arranged by size:
2040 1917 1384 846 374 268 204
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6836
AflIII A'CryG_T
Cuts at: 1203 3302 5932 6909 1203
Size: 2099 2630 977 1327
Fragments arranged by size:
2630 2099 1327 977
AgeI A'CCGG_T
Cuts at: 5864 5864
Size: 7033
AhdI GACnn_n'nnGTC
Cuts at: 4111 5762 4111
Size: 1651 5382
AleI CACnn'nnGTG
Cuts at: 1166 3096 1166
Size: 1930 5103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7033
AlwNI CAG_nnn'CTG
Cuts at: 3860 3959 3962 4776 6348 3860
Size: 99 3 814 1572 4545
Fragments arranged by size:
4545 1572 814 99 3
ApaI G_GGCC'C
Cuts at: 2774 2774
Size: 7033
ApaLI G'TGCA_C
Cuts at: 3179 5090 6246 3179
Size: 1911 1156 3966
Fragments arranged by size:
3966 1911 1156
ApoI r'AATT_y
Cuts at: 1493 3464 4342 4568 4720 6829 1493
Size: 1971 878 226 152 2109 1697
Fragments arranged by size:
2109 1971 1697 878 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4172 5587 160
Size: 872 3140 1415 1606
Fragments arranged by size:
3140 1606 1415 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
AvaI C'yCGr_G
Cuts at: 1870 1943 2341 2767 4358 1870
Size: 73 398 426 1591 4545
Fragments arranged by size:
4545 1591 426 398 73
BamHI G'GATC_C
Cuts at: 3586 4363 4730 3586
Size: 777 367 5889
Fragments arranged by size:
5889 777 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2854 4354 5809 618
Size: 325 217 627 363 704 1500 1455 1842
Fragments arranged by size:
1842 1500 1455 704 627 363 325 217
BanII G_rGCy'C
Cuts at: 1391 2774 3120 4352 1391
Size: 1383 346 1232 4072
Fragments arranged by size:
4072 1383 1232 346
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2076 2232 3875 4311 928
Size: 971 177 156 1643 436 3650
Fragments arranged by size:
3650 1643 971 436 177 156
BbvCI CC'TCA_GC
Cuts at: 2171 2171
Size: 7033
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5224 5258 1903
Size: 34 3287 34 3678
Fragments arranged by size:
3678 3287 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3029 4925 6135 3029
Size: 1896 1210 3927
Fragments arranged by size:
3927 1896 1210
BclI T'GATC_A
Cuts at: 1548 2425 3568 1548
Size: 877 1143 5013
Fragments arranged by size:
5013 1143 877
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2778 5644 136
Size: 107 122 71 631 605 1106 2866 1525
Fragments arranged by size:
2866 1525 1106 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7028 7028
Size: 7033
BlpI GC'TnA_GC
Cuts at: 4450 4450
Size: 7033
Bme1580I G_kGCm'C
Cuts at: 1790 2155 2774 3161 3183 5094 6250 1790
Size: 365 619 387 22 1911 1156 2573
Fragments arranged by size:
2573 1911 1156 619 387 365 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3009 4764 5722
Size: 416 53 83 399 24 1592 1755 958
Cuts at: 5722 442
Size: 1753
Fragments arranged by size:
1755 1753 1592 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3137 1056
Size: 2081 4952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2552 2552
Size: 7033
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2562 2592 3134 3268 3321 3832 3966 4007
Size: 1323 30 542 134 53 511 134 41
Cuts at: 4007 5693 1239
Size: 1686 2579
Fragments arranged by size:
2579 1686 1323 542 511 134 134 53
41 30
Bpu10I CC'TnA_GC
Cuts at: 2171 3054 3818 3966 2171
Size: 883 764 148 5238
Fragments arranged by size:
5238 883 764 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3914 5158 6023 6321 3914
Size: 1244 865 298 4626
Fragments arranged by size:
4626 1244 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2213 3286 5696 882
Size: 617 714 1073 2410 2219
Fragments arranged by size:
2410 2219 1073 714 617
BsaAI yAC'GTr
Cuts at: 493 1204 3303 4824 493
Size: 711 2099 1521 2702
Fragments arranged by size:
2702 2099 1521 711
BsaBI GATnn'nnATC
Cuts at: 3419 7027 3419
Size: 3608 3425
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2532 4053 4288 4840
Size: 53 83 186 936 999 1521 235 552
Cuts at: 4840 5222 5857 275
Size: 382 635 1451
Fragments arranged by size:
1521 1451 999 936 635 552 382 235
186 83 53
BsaWI w'CCGG_w
Cuts at: 2060 5466 5864 6138 6285 6696 2060
Size: 3406 398 274 147 411 2397
Fragments arranged by size:
3406 2397 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2103 2133 3926 3956 1178
Size: 30 165 30 700 30 1793 30 4255
Fragments arranged by size:
4255 1793 700 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3621 1186
Size: 2435 4598
BseYI C'CCAG_C
Cuts at: 2304 2942 3294 3908 6236 6732 2304
Size: 638 352 614 2328 496 2605
Fragments arranged by size:
2605 2328 638 614 496 352
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2285 2621 2990 1163
Size: 573 549 336 369 5206
Fragments arranged by size:
5206 573 549 369 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4483 5244 6272 1540
Size: 275 186 2482 761 1028 2301
Fragments arranged by size:
2482 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3052 3120 3183 3779 5094 5179 6250 3052
Size: 68 63 596 1315 85 1071 3835
Fragments arranged by size:
3835 1315 1071 596 85 68 63
BsmI GAATG_Cn'
Cuts at: 2137 4544 4637 2137
Size: 2407 93 4533
Fragments arranged by size:
4533 2407 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3007 3007
Size: 7033
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2155 2774 3052 3120 3161 3183 3779
Size: 399 365 619 278 68 41 22 596
Cuts at: 3779 4352 5094 5179 6250 1391
Size: 573 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 619 596 573 399 365
278 85 68 41 22
BspEI T'CCGG_A
Cuts at: 2060 6696 2060
Size: 4636 2397
BspHI T'CATG_A
Cuts at: 4922 6652 4922
Size: 1730 5303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2194 2284 2414 3362 4388 844
Size: 1350 90 130 948 1026 3489
Fragments arranged by size:
3489 1350 1026 948 130 90
BsrBI CCG'CTC
Cuts at: 1394 1823 4920 1394
Size: 429 3097 3507
Fragments arranged by size:
3507 3097 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5528 5702 65
Size: 1183 4280 174 1396
Fragments arranged by size:
4280 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5677 5864 1700
Size: 110 3867 187 2869
Fragments arranged by size:
3867 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7033
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7033
BssSI C'ACGA_G
Cuts at: 5093 6105 5093
Size: 1012 6021
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3962 794
Size: 3168 3865
BstBI TT'CG_AA
Cuts at: 5837 5837
Size: 7033
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7033
BstXI CCAn_nnnn'nTGG
Cuts at: 3098 3161 3716 3098
Size: 63 555 6415
Fragments arranged by size:
6415 555 63
BstYI r'GATC_y
Cuts at: 1972 2362 2996 3586 4202 4363 4730 5115 5132
Size: 390 634 590 616 161 367 385 17
Cuts at: 5132 6584 6670 6682 7028 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 634 616 590 390 385 367
346 161 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3633 3633
Size: 7033
BtgI C'CryG_G
Cuts at: 513 1065 2004 3837 513
Size: 552 939 1833 3709
Fragments arranged by size:
3709 1833 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6294
Fragments arranged by size:
6294 683 56
BtsI GCAGTG_nn'
Cuts at: 2760 2982 3024 3103 4549 5343 5363 2760
Size: 222 42 79 1446 794 20 4430
Fragments arranged by size:
4430 1446 794 222 79 42 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7033
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6998
DraI TTT'AAA
Cuts at: 4337 4337
Size: 7033
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6040 809
Size: 507 4724 1802
Fragments arranged by size:
4724 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3508 4480 5369
Size: 54 1425 50 275 18 1678 972 889
Cuts at: 5369 6998 8
Size: 1629 43
Fragments arranged by size:
1678 1629 1425 972 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4480 1537
Size: 275 2668 4090
Fragments arranged by size:
4090 2668 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3342 4963 1353
Size: 1989 1621 3423
Fragments arranged by size:
3423 1989 1621
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5620 5994 6140 387
Size: 1064 138 4031 374 146 1280
Fragments arranged by size:
4031 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3118 3118
Size: 7033
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2478 2562 2592 2682 3056 3134 3268
Size: 393 846 84 30 90 374 78 134
Cuts at: 3268 3321 3832 3966 4007 5096 5693 6480 6748
Size: 53 511 134 41 1089 597 787 268
Cuts at: 6748 1239
Size: 1524
Fragments arranged by size:
1524 1089 846 787 597 511 393 374
268 134 134 90 84 78 53 41
30
EcoNI CCTnn'n_nnAGG
Cuts at: 2201 2526 2201
Size: 325 6708
EcoO109I rG'GnC_Cy
Cuts at: 2703 2771 3253 2703
Size: 68 482 6483
Fragments arranged by size:
6483 482 68
EcoRI G'AATT_C
Cuts at: 4342 4720 4342
Size: 378 6655
FspI TGC'GCA
Cuts at: 5539 5539
Size: 7033
HaeII r_GCGC'y
Cuts at: 1678 6180 1678
Size: 4502 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 2543 2575 2981
Size: 32 135 32 531 32 408 32 406
Cuts at: 2981 3013 3571 3603 3862 3894 4097 4129 5674
Size: 32 558 32 259 32 203 32 1545
Cuts at: 5674 5706 5748 5780 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1545 558 531 408 406 259 203
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 4377 4623 669
Size: 988 281 1416 1023 246 3079
Fragments arranged by size:
3079 1416 1023 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7033
HpaI GTT'AAC
Cuts at: 4623 4623
Size: 7033
KpnI G_GTAC'C
Cuts at: 4358 4358
Size: 7033
MfeI C'AATT_G
Cuts at: 4632 4632
Size: 7033
MluI A'CGCG_T
Cuts at: 6909 6909
Size: 7033
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3241 4309 6147 6331 1305
Size: 1936 1068 1838 184 2007
Fragments arranged by size:
2007 1936 1838 1068 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5211
Fragments arranged by size:
5211 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3096 4991 5350 5509 518
Size: 648 1930 1895 359 159 2042
Fragments arranged by size:
2042 1930 1895 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2186 2308 2860 3076 3194 3200 3734 3959
Size: 580 122 552 216 118 6 534 225
Cuts at: 3959 4027 4449 5126 6274 6519 1606
Size: 68 422 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 580 552 534 422 245
225 216 122 118 68 6
NcoI C'CATG_G
Cuts at: 513 1065 2004 3837 513
Size: 552 939 1833 3709
Fragments arranged by size:
3709 1833 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7033
NheI G'CTAG_C
Cuts at: 1052 3133 1052
Size: 2081 4952
NotI GC'GGCC_GC
Cuts at: 4480 4480
Size: 7033
NruI TCG'CGA
Cuts at: 4127 4127
Size: 7033
NspI r_CATG'y
Cuts at: 1132 1229 2835 3432 4391 5936 1132
Size: 97 1606 597 959 1545 2229
Fragments arranged by size:
2229 1606 1545 959 597 97
PasI CC'CwG_GG
Cuts at: 1348 2178 4117 1348
Size: 830 1939 4264
Fragments arranged by size:
4264 1939 830
PciI A'CATG_T
Cuts at: 5932 5932
Size: 7033
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3112 3860 1108
Size: 2004 748 4281
Fragments arranged by size:
4281 2004 748
PfoI T'CCnGG_A
Cuts at: 3982 3982
Size: 7033
PmeI GTTT'AAAC
Cuts at: 4337 4337
Size: 7033
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2154 2186 5102 5134 6615 6647 2154
Size: 32 2916 32 1481 32 2540
Fragments arranged by size:
2916 2540 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2703 3253 2703
Size: 550 6483
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7033
PsiI TTA'TAA
Cuts at: 4603 4603
Size: 7033
PspOMI G'GGCC_C
Cuts at: 2770 2770
Size: 7033
PstI C_TGCA'G
Cuts at: 830 2161 2353 3199 4278 4385 830
Size: 1331 192 846 1079 107 3478
Fragments arranged by size:
3478 1331 1079 846 192 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7033
PvuII CAG'CTG
Cuts at: 2186 2308 3076 3194 3200 3734 3959 4027 2186
Size: 122 768 118 6 534 225 68 5192
Fragments arranged by size:
5192 768 534 225 122 118 68 6
SacI G_AGCT'C
Cuts at: 3120 3120
Size: 7033
SalI G'TCGA_C
Cuts at: 1936 4375 1936
Size: 2439 4594
SbfI CC_TGCA'GG
Cuts at: 4385 4385
Size: 7033
ScaI AGT'ACT
Cuts at: 5281 6993 5281
Size: 1712 5321
SexAI A'CCwGG_T
Cuts at: 3244 3244
Size: 7033
SfcI C'TryA_G
Cuts at: 826 1045 2157 2349 2649 3195 3207 4274 4381
Size: 219 1112 192 300 546 12 1067 107
Cuts at: 4381 5516 6197 6388 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1112 1067 681 546 300 219
192 191 107 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7033
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7033
SmaI CCC'GGG
Cuts at: 1872 4360 1872
Size: 2488 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3929 5137 6038 6300 820
Size: 197 926 1986 1208 901 262 1553
Fragments arranged by size:
1986 1553 1208 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4824 493
Size: 4331 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7033
SphI G_CATG'C
Cuts at: 1132 3432 4391 1132
Size: 2300 959 3774
Fragments arranged by size:
3774 2300 959
SspI AAT'ATT
Cuts at: 5 52 4957 5
Size: 47 4905 2081
Fragments arranged by size:
4905 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7033
StyI C'CwwG_G
Cuts at: 513 1065 2004 2776 3837 4101 4472 513
Size: 552 939 772 1061 264 371 3074
Fragments arranged by size:
3074 1061 939 772 552 371 264
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2240 3830 5060 5077 5230 5415 5860
Size: 1255 660 1590 1230 17 153 185 445
Cuts at: 5860 325
Size: 1498
Fragments arranged by size:
1590 1498 1255 1230 660 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5279 6991 96
Size: 274 80 33 51 1443 3302 1712 138
Fragments arranged by size:
3302 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4043 4075 6993 7025 4043
Size: 32 2918 32 4051
Fragments arranged by size:
4051 2918 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7033
XbaI T'CTAG_A
Cuts at: 4369 4369
Size: 7033
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7033
XmaI C'CCGG_G
Cuts at: 1870 4358 1870
Size: 2488 4545
XmnI GAAnn'nnTTC
Cuts at: 5162 5162
Size: 7033
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4054 4841 276
Size: 53 83 186 936 2520 787 2468
Fragments arranged by size:
2520 2468 936 787 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI PmlI PspXI PsrI RsrII
SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI