Restriction map for
pFN21AA2015
(Circular) (Six-base) MAPSORT of: pFN21AA2015.seq Check: 7517 from: 1 to: 7681
pFN21AA2015 7681 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5491 278
Size: 53 83 186 936 3955 2468
Fragments arranged by size:
3955 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5024 1937
Size: 3087 4594
Acc65I G'GTAC_C
Cuts at: 5002 5002
Size: 7681
AclI AA'CG_TT
Cuts at: 1563 5808 6181 1563
Size: 4245 373 3063
Fragments arranged by size:
4245 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2874 3276 3812 4032 4272 5744 7128
Size: 370 872 402 536 220 240 1472 1384
Cuts at: 7128 7396 1632
Size: 268 1917
Fragments arranged by size:
1917 1472 1384 872 536 402 370 268
240 220
AfeI AGC'GCT
Cuts at: 2159 2159
Size: 7681
AflII C'TTAA_G
Cuts at: 820 1017 4418 820
Size: 197 3401 4083
Fragments arranged by size:
4083 3401 197
AflIII A'CryG_T
Cuts at: 1203 2694 6580 7557 1203
Size: 1491 3886 977 1327
Fragments arranged by size:
3886 1491 1327 977
AgeI A'CCGG_T
Cuts at: 6512 6512
Size: 7681
AhdI GACnn_n'nnGTC
Cuts at: 6410 6410
Size: 7681
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7681
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7681
AlwNI CAG_nnn'CTG
Cuts at: 3741 5424 6996 3741
Size: 1683 1572 4426
Fragments arranged by size:
4426 1683 1572
ApaLI G'TGCA_C
Cuts at: 5738 6894 5738
Size: 1156 6525
ApoI r'AATT_y
Cuts at: 1493 2530 2555 2757 2836 2983 3208 3872 4002
Size: 1037 25 202 79 147 225 664 130
Cuts at: 4002 4028 4631 4682 4914 4990 5216 5368 7477
Size: 26 603 51 232 76 226 152 2109
Cuts at: 7477 1493
Size: 1697
Fragments arranged by size:
2109 1697 1037 664 603 232 226 225
202 152 147 130 79 76 51 26
25
AseI AT'TA_AT
Cuts at: 160 1032 3726 3791 3954 4049 4739 6235 160
Size: 872 2694 65 163 95 690 1496 1606
Fragments arranged by size:
2694 1606 1496 872 690 163 95 65
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7681
AvaI C'yCGr_G
Cuts at: 1870 1943 3441 5006 1870
Size: 73 1498 1565 4545
Fragments arranged by size:
4545 1565 1498 73
BamHI G'GATC_C
Cuts at: 5011 5378 5011
Size: 367 7314
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2375 5002 6457 618
Size: 325 217 627 588 2627 1455 1842
Fragments arranged by size:
2627 1842 1455 627 588 325 217
BanII G_rGCy'C
Cuts at: 1391 2057 5000 1391
Size: 666 2943 4072
Fragments arranged by size:
4072 2943 666
BbeI G_GCGC'C
Cuts at: 2379 2379
Size: 7681
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4278 928
Size: 971 2379 4331
Fragments arranged by size:
4331 2379 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2702 2736 5872 5906 1903
Size: 34 765 34 3136 34 3678
Fragments arranged by size:
3678 3136 765 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5573 6783 5573
Size: 1210 6471
BclI T'GATC_A
Cuts at: 1548 4767 1548
Size: 3219 4462
BfrBI ATG'CAT
Cuts at: 3577 4163 3577
Size: 586 7095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6292 136
Size: 107 122 71 631 605 4620 1525
Fragments arranged by size:
4620 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3763 7676 3763
Size: 3913 3768
BlpI GC'TnA_GC
Cuts at: 5098 5098
Size: 7681
Bme1580I G_kGCm'C
Cuts at: 1790 2236 5742 6898 1790
Size: 446 3506 1156 2573
Fragments arranged by size:
3506 2573 1156 446
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7681
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2110 5412 6370
Size: 416 53 83 399 24 693 3302 958
Cuts at: 6370 442
Size: 1753
Fragments arranged by size:
3302 1753 958 693 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7681
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3509
Size: 7681
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 6341 1239
Size: 5102 2579
Bpu10I CC'TnA_GC
Cuts at: 2051 2051
Size: 7681
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2673 3767 4397 5806 6671 6969 2673
Size: 1094 630 1409 865 298 3385
Fragments arranged by size:
3385 1409 1094 865 630 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6344 882
Size: 617 4845 2219
Fragments arranged by size:
4845 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2678 5472 493
Size: 711 1474 2794 2702
Fragments arranged by size:
2794 2702 1474 711
BsaBI GATnn'nnATC
Cuts at: 2288 7675 2288
Size: 5387 2294
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2032 2142 2190 2376
Size: 53 83 186 936 499 110 48 186
Cuts at: 2376 5488 5870 6505 275
Size: 3112 382 635 1451
Fragments arranged by size:
3112 1451 936 635 499 382 186 186
110 83 53 48
BsaWI w'CCGG_w
Cuts at: 6114 6512 6786 6933 7344 6114
Size: 398 274 147 411 6451
Fragments arranged by size:
6451 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7456
Fragments arranged by size:
7456 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3647 1186
Size: 2461 5220
BseYI C'CCAG_C
Cuts at: 6884 7380 6884
Size: 496 7185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 4517 1163
Size: 573 516 2265 4327
Fragments arranged by size:
4327 2265 573 516
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5131 5892 6920 1540
Size: 275 186 3130 761 1028 2301
Fragments arranged by size:
3130 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2057 5742 5827 6898 2057
Size: 3685 85 1071 2840
Fragments arranged by size:
3685 2840 1071 85
BsmI GAATG_Cn'
Cuts at: 3202 5192 5285 3202
Size: 1990 93 5598
Fragments arranged by size:
5598 1990 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2023 2023
Size: 7681
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2057 2236 5000 5742 5827 6898 1391
Size: 399 267 179 2764 742 85 1071 2174
Fragments arranged by size:
2764 2174 1071 742 399 267 179 85
BspEI T'CCGG_A
Cuts at: 7344 7344
Size: 7681
BspHI T'CATG_A
Cuts at: 5570 7300 5570
Size: 1730 5951
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2163 5036 844
Size: 1319 2873 3489
Fragments arranged by size:
3489 2873 1319
BsrBI CCG'CTC
Cuts at: 1394 1823 5568 1394
Size: 429 3745 3507
Fragments arranged by size:
3745 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3003 6176 6350 65
Size: 1183 1755 3173 174 1396
Fragments arranged by size:
3173 1755 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6325 6512 1700
Size: 110 4515 187 2869
Fragments arranged by size:
4515 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2329 3523 4354 96
Size: 2233 1194 831 3423
Fragments arranged by size:
3423 2233 1194 831
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7681
BssSI C'ACGA_G
Cuts at: 5741 6753 5741
Size: 1012 6669
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7681
BstBI TT'CG_AA
Cuts at: 2797 6485 2797
Size: 3688 3993
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7681
BstYI r'GATC_y
Cuts at: 1972 2115 3763 5011 5378 5763 5780 7232 7318
Size: 143 1648 1248 367 385 17 1452 86
Cuts at: 7318 7330 7676 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1648 1452 1248 385 367 346 143
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3536 3536
Size: 7681
BtgI C'CryG_G
Cuts at: 513 1065 2058 2145 2236 4189 513
Size: 552 993 87 91 1953 4005
Fragments arranged by size:
4005 1953 993 552 91 87
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2987 487
Size: 683 56 1761 5181
Fragments arranged by size:
5181 1761 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 3285 4500 4713 4770 5197 5991 6011 2409
Size: 876 1215 213 57 427 794 20 4079
Fragments arranged by size:
4079 1215 876 794 427 213 57 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7681
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3135 3170 529
Size: 35 2571 35 5040
Fragments arranged by size:
5040 2571 35 35
DraI TTT'AAA
Cuts at: 3813 4985 3813
Size: 1172 6509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6688 809
Size: 507 5372 1802
Fragments arranged by size:
5372 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2239 2701 4266
Size: 54 1425 50 275 18 409 462 1565
Cuts at: 4266 4305 4905 5128 6017 7646 8
Size: 39 600 223 889 1629 43
Fragments arranged by size:
1629 1565 1425 889 600 462 409 275
223 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 5128 1537
Size: 275 3316 4090
Fragments arranged by size:
4090 3316 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2337 2689 4104 4653 4827 5611 1353
Size: 668 316 352 1415 549 174 784 3423
Fragments arranged by size:
3423 1415 784 668 549 352 316 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6268 6642 6788 387
Size: 1064 138 4679 374 146 1280
Fragments arranged by size:
4679 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2055 2055
Size: 7681
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2874 3276 3812 4032 4272 5744
Size: 393 370 872 402 536 220 240 1472
Cuts at: 5744 6341 7128 7396 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1472 872 787 597 536 402 393
370 268 240 220
EcoNI CCTnn'n_nnAGG
Cuts at: 2039 2039
Size: 7681
EcoRI G'AATT_C
Cuts at: 3208 4028 4914 4990 5368 3208
Size: 820 886 76 378 5521
Fragments arranged by size:
5521 886 820 378 76
EcoRV GAT'ATC
Cuts at: 2398 2398
Size: 7681
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4711 4975 4711
Size: 264 7417
FspI TGC'GCA
Cuts at: 6187 6187
Size: 7681
HaeII r_GCGC'y
Cuts at: 1678 2048 2161 2379 6828 1678
Size: 370 113 218 4449 2531
Fragments arranged by size:
4449 2531 370 218 113
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2268 2300 3628 3660 4325
Size: 32 135 32 696 32 1328 32 665
Cuts at: 4325 4357 6322 6354 6396 6428 1373
Size: 32 1965 32 42 32 2626
Fragments arranged by size:
2626 1965 1328 696 665 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2593 3647 5025 5271 669
Size: 988 281 655 1054 1378 246 3079
Fragments arranged by size:
3079 1378 1054 988 655 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7681
HpaI GTT'AAC
Cuts at: 5271 5271
Size: 7681
KasI G'GCGC_C
Cuts at: 2375 2375
Size: 7681
KpnI G_GTAC'C
Cuts at: 5006 5006
Size: 7681
MfeI C'AATT_G
Cuts at: 4183 5280 4183
Size: 1097 6584
MluI A'CGCG_T
Cuts at: 7557 7557
Size: 7681
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2773 4377 6795 6979 1305
Size: 1468 1604 2418 184 2007
Fragments arranged by size:
2418 2007 1604 1468 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2703 4307 10
Size: 54 1425 343 871 1604 3384
Fragments arranged by size:
3384 1604 1425 871 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2241 3008 3582 5639 5998 6157 518
Size: 648 1075 767 574 2057 359 159 2042
Fragments arranged by size:
2057 2042 1075 767 648 574 359 159
MspA1I CmG'CkG
Cuts at: 1606 2147 3529 4798 5097 5774 6922 7167 1606
Size: 541 1382 1269 299 677 1148 245 2120
Fragments arranged by size:
2120 1382 1269 1148 677 541 299 245
NarI GG'CG_CC
Cuts at: 2376 2376
Size: 7681
NcoI C'CATG_G
Cuts at: 513 1065 2058 2236 4189 513
Size: 552 993 178 1953 4005
Fragments arranged by size:
4005 1953 993 552 178
NdeI CA'TA_TG
Cuts at: 387 2547 4428 387
Size: 2160 1881 3640
Fragments arranged by size:
3640 2160 1881
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7681
NotI GC'GGCC_GC
Cuts at: 5128 5128
Size: 7681
NsiI A_TGCA'T
Cuts at: 3579 4165 3579
Size: 586 7095
NspI r_CATG'y
Cuts at: 1132 1229 2588 2698 5039 6584 1132
Size: 97 1359 110 2341 1545 2229
Fragments arranged by size:
2341 2229 1545 1359 110 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7681
PciI A'CATG_T
Cuts at: 2694 6580 2694
Size: 3886 3795
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7681
PfoI T'CCnGG_A
Cuts at: 2178 2364 2534 2178
Size: 186 170 7325
Fragments arranged by size:
7325 186 170
PmeI GTTT'AAAC
Cuts at: 4985 4985
Size: 7681
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5750 5782 7263 7295 5750
Size: 32 1481 32 6136
Fragments arranged by size:
6136 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7681
PsiI TTA'TAA
Cuts at: 5251 5251
Size: 7681
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4830 4862 4830
Size: 32 7649
PstI C_TGCA'G
Cuts at: 830 5033 830
Size: 4203 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7681
PvuII CAG'CTG
Cuts at: 3529 3529
Size: 7681
SacI G_AGCT'C
Cuts at: 2057 2057
Size: 7681
SacII CC_GC'GG
Cuts at: 2148 2148
Size: 7681
SalI G'TCGA_C
Cuts at: 1936 5023 1936
Size: 3087 4594
SapI GCTCTTCn'nnn_
Cuts at: 2021 2021
Size: 7681
SbfI CC_TGCA'GG
Cuts at: 5033 5033
Size: 7681
ScaI AGT'ACT
Cuts at: 5929 7641 5929
Size: 1712 5969
SfcI C'TryA_G
Cuts at: 826 1045 5029 6164 6845 7036 826
Size: 219 3984 1135 681 191 1471
Fragments arranged by size:
3984 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2377 2377
Size: 7681
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7681
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7681
SmaI CCC'GGG
Cuts at: 1872 5008 1872
Size: 3136 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2688 3746 4376 4418 5785 6686
Size: 197 926 745 1058 630 42 1367 901
Cuts at: 6686 6948 820
Size: 262 1553
Fragments arranged by size:
1553 1367 1058 926 901 745 630 262
197 42
SnaBI TAC'GTA
Cuts at: 493 2678 5472 493
Size: 2185 2794 2702
Fragments arranged by size:
2794 2702 2185
SpeI A'CTAG_T
Cuts at: 152 3334 152
Size: 3182 4499
SphI G_CATG'C
Cuts at: 1132 5039 1132
Size: 3907 3774
SspI AAT'ATT
Cuts at: 5 52 2482 3388 4808 5605 5
Size: 47 2430 906 1420 797 2081
Fragments arranged by size:
2430 2081 1420 906 797 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7681
StyI C'CwwG_G
Cuts at: 513 1065 2058 2236 4189 5120 513
Size: 552 993 178 1953 931 3074
Fragments arranged by size:
3074 1953 993 931 552 178
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5708 5725 5878 6063 6508 325
Size: 1255 4128 17 153 185 445 1498
Fragments arranged by size:
4128 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2329 2626 3300
Size: 274 80 33 51 1443 352 297 674
Cuts at: 3300 3523 4354 5927 7639 96
Size: 223 831 1573 1712 138
Fragments arranged by size:
1712 1573 1443 831 674 352 297 274
223 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7641 7673 7641
Size: 32 7649
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7681
XbaI T'CTAG_A
Cuts at: 3362 3769 5017 3362
Size: 407 1248 6026
Fragments arranged by size:
6026 1248 407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2362 2362
Size: 7681
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7681
XmaI C'CCGG_G
Cuts at: 1870 5006 1870
Size: 3136 4545
XmnI GAAnn'nnTTC
Cuts at: 2015 3765 4323 5810 2015
Size: 1750 558 1487 3886
Fragments arranged by size:
3886 1750 1487 558
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5489 276
Size: 53 83 186 936 3955 2468
Fragments arranged by size:
3955 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BsiWI
BstXI BstZ17I DraIII EcoO109I FseI FspAI NaeI NgoMIV
NruI PacI PmlI PpuMI PspOMI PspXI RsrII SanDI
SexAI SfiI SrfI SwaI