Restriction map for
pFN21AA2011
(Circular) (Six-base) MAPSORT of: pFN21AA2011.seq Check: 5905 from: 1 to: 7777
pFN21AA2011 7777 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3004 3065 4702 3004
Size: 61 1637 6079
Fragments arranged by size:
6079 1637 61
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5587 278
Size: 53 83 186 936 4051 2468
Fragments arranged by size:
4051 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4275 5120 1937
Size: 2338 845 4594
Fragments arranged by size:
4594 2338 845
Acc65I G'GTAC_C
Cuts at: 3410 5098 3410
Size: 1688 6089
AclI AA'CG_TT
Cuts at: 1563 5904 6277 1563
Size: 4341 373 3063
Fragments arranged by size:
4341 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3138 3700 3745 3863 3948 4757 4830 4913
Size: 1506 562 45 118 85 809 73 83
Cuts at: 4913 5840 7224 7492 1632
Size: 927 1384 268 1917
Fragments arranged by size:
1917 1506 1384 927 809 562 268 118
85 83 73 45
AfeI AGC'GCT
Cuts at: 4684 4875 4684
Size: 191 7586
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7580
AflIII A'CryG_T
Cuts at: 1203 2188 6676 7653 1203
Size: 985 4488 977 1327
Fragments arranged by size:
4488 1327 985 977
AgeI A'CCGG_T
Cuts at: 6608 6608
Size: 7777
AhdI GACnn_n'nnGTC
Cuts at: 3643 6506 3643
Size: 2863 4914
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7777
AlwNI CAG_nnn'CTG
Cuts at: 2984 3685 4316 4640 5520 7092 2984
Size: 701 631 324 880 1572 3669
Fragments arranged by size:
3669 1572 880 701 631 324
ApaI G_GGCC'C
Cuts at: 2293 2600 2975 3127 4291 2293
Size: 307 375 152 1164 5779
Fragments arranged by size:
5779 1164 375 307 152
ApaLI G'TGCA_C
Cuts at: 4304 5834 6990 4304
Size: 1530 1156 5091
Fragments arranged by size:
5091 1530 1156
ApoI r'AATT_y
Cuts at: 1493 2726 5086 5312 5464 7573 1493
Size: 1233 2360 226 152 2109 1697
Fragments arranged by size:
2360 2109 1697 1233 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6331 160
Size: 872 5299 1606
Fragments arranged by size:
5299 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7777
AvaI C'yCGr_G
Cuts at: 1870 1943 2169 2285 2679 4256 4335 4558 5016
Size: 73 226 116 394 1577 79 223 458
Cuts at: 5016 5102 1870
Size: 86 4545
Fragments arranged by size:
4545 1577 458 394 226 223 116 86
79 73
AvrII C'CTAG_G
Cuts at: 4079 4079
Size: 7777
BamHI G'GATC_C
Cuts at: 4368 5107 5474 4368
Size: 739 367 6671
Fragments arranged by size:
6671 739 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2015 2095 2254 2875 3254
Size: 325 217 627 228 80 159 621 379
Cuts at: 3254 3410 3738 3950 5098 6553 618
Size: 156 328 212 1148 1455 1842
Fragments arranged by size:
1842 1455 1148 627 621 379 328 325
228 217 212 159 156 80
BanII G_rGCy'C
Cuts at: 1391 2108 2293 2300 2600 2810 2822 2975 3127
Size: 717 185 7 300 210 12 153 152
Cuts at: 3127 3185 3317 3501 4291 4443 5023 5096 1391
Size: 58 132 184 790 152 580 73 4072
Fragments arranged by size:
4072 790 717 580 300 210 185 184
153 152 152 132 73 58 12 7
BbeI G_GCGC'C
Cuts at: 3954 3954
Size: 7777
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3793 4334 4504 4534 928
Size: 971 1894 541 170 30 4171
Fragments arranged by size:
4171 1894 971 541 170 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5968 6002 1903
Size: 34 4031 34 3678
Fragments arranged by size:
4031 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2656 5669 6879 2656
Size: 3013 1210 3554
Fragments arranged by size:
3554 3013 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7777
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2942 2979 3482
Size: 107 122 71 631 605 1270 37 503
Cuts at: 3482 3731 6388 136
Size: 249 2657 1525
Fragments arranged by size:
2657 1525 1270 631 605 503 249 122
107 71 37
BglII A'GATC_T
Cuts at: 7772 7772
Size: 7777
BlpI GC'TnA_GC
Cuts at: 2766 2922 5022 5194 2766
Size: 156 2100 172 5349
Fragments arranged by size:
5349 2100 172 156
Bme1580I G_kGCm'C
Cuts at: 1790 2100 2257 2293 2600 2880 2975 3127 3165
Size: 310 157 36 307 280 95 152 38
Cuts at: 3165 4291 4308 5838 6994 1790
Size: 1126 17 1530 1156 2573
Fragments arranged by size:
2573 1530 1156 1126 310 307 280 157
152 95 38 36 17
BmgBI CAC'GTC
Cuts at: 1318 2189 4510 1318
Size: 871 2321 4585
Fragments arranged by size:
4585 2321 871
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2192 2280 5508
Size: 416 53 83 399 24 775 88 3228
Cuts at: 5508 6466 442
Size: 958 1753
Fragments arranged by size:
3228 1753 958 775 416 399 88 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7777
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 4179 2956
Size: 1223 6554
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2346 3254 3302 3689 4070 4216 4586 4859
Size: 1107 908 48 387 381 146 370 273
Cuts at: 4859 4963 4990 6437 1239
Size: 104 27 1447 2579
Fragments arranged by size:
2579 1447 1107 908 387 381 370 273
146 104 48 27
Bpu10I CC'TnA_GC
Cuts at: 2604 2758 3569 2604
Size: 154 811 6812
Fragments arranged by size:
6812 811 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2392 2794 3134 3773 4076 4358 4427 5902 6767
Size: 402 340 639 303 282 69 1475 865
Cuts at: 6767 7065 2392
Size: 298 3104
Fragments arranged by size:
3104 1475 865 639 402 340 303 298
282 69
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3860 6440 882
Size: 617 2361 2580 2219
Fragments arranged by size:
2580 2361 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5568 493
Size: 711 4364 2702
Fragments arranged by size:
4364 2702 711
BsaBI GATnn'nnATC
Cuts at: 7771 7771
Size: 7777
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3951 5584 5966 6601
Size: 53 83 186 936 2418 1633 382 635
Cuts at: 6601 275
Size: 1451
Fragments arranged by size:
2418 1633 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2442 3400 6210 6608 6882 7029 7440 2442
Size: 958 2810 398 274 147 411 2779
Fragments arranged by size:
2810 2779 958 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7552
Fragments arranged by size:
7552 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2394 2505 2563 2680 2945 3467 3470 3473
Size: 1208 111 58 117 265 522 3 3
Cuts at: 3473 4160 4163 4418 4721 4922 1186
Size: 687 3 255 303 201 4041
Fragments arranged by size:
4041 1208 687 522 303 265 255 201
117 111 58 3 3 3
BseYI C'CCAG_C
Cuts at: 2110 2382 2419 2590 2753 2960 3032 3535 3776
Size: 272 37 171 163 207 72 503 241
Cuts at: 3776 3903 4633 4679 4776 4780 6980 7476 2110
Size: 127 730 46 97 4 2200 496 2411
Fragments arranged by size:
2411 2200 730 503 496 272 241 207
171 163 127 97 72 46 37 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3336 1163
Size: 573 1600 5604
Fragments arranged by size:
5604 1600 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5227 5988 7016 1540
Size: 275 186 3226 761 1028 2301
Fragments arranged by size:
3226 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2300 4308 4443 4767 4952 5838 5923 6994 2300
Size: 2008 135 324 185 886 85 1071 3083
Fragments arranged by size:
3083 2008 1071 886 324 185 135 85
BsmI GAATG_Cn'
Cuts at: 4453 5288 5381 4453
Size: 835 93 6849
Fragments arranged by size:
6849 835 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4150 4427 4150
Size: 277 7500
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 2108 2257 2293 2300 2600 2810
Size: 399 310 8 149 36 7 300 210
Cuts at: 2810 2822 2880 2975 3127 3165 3185 3317 3501
Size: 12 58 95 152 38 20 132 184
Cuts at: 3501 4291 4308 4443 4767 4952 5023 5096 5838
Size: 790 17 135 324 185 71 73 742
Cuts at: 5838 5923 6994 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 790 742 399 324 310 300
210 185 184 152 149 135 132 95
85 73 71 58 38 36 20 17
12 8 7
BspEI T'CCGG_A
Cuts at: 7440 7440
Size: 7777
BspHI T'CATG_A
Cuts at: 5666 7396 5666
Size: 1730 6047
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2147 2365 2641 3004 3065 4034 4401 4527
Size: 1303 218 276 363 61 969 367 126
Cuts at: 4527 4702 5132 844
Size: 175 430 3489
Fragments arranged by size:
3489 1303 969 430 367 363 276 218
175 126 61
BsrBI CCG'CTC
Cuts at: 1394 1823 2926 3616 5664 1394
Size: 429 1103 690 2048 3507
Fragments arranged by size:
3507 2048 1103 690 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3055 6272 6446 65
Size: 1183 1807 3217 174 1396
Fragments arranged by size:
3217 1807 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6421 6608 1700
Size: 110 4611 187 2869
Fragments arranged by size:
4611 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2204 2524 4101 96
Size: 2108 320 1577 3772
Fragments arranged by size:
3772 2108 1577 320
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7777
BssSI C'ACGA_G
Cuts at: 4479 4724 5837 6849 4479
Size: 245 1113 1012 5407
Fragments arranged by size:
5407 1113 1012 245
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4026 794
Size: 3232 4545
BstBI TT'CG_AA
Cuts at: 3222 6581 3222
Size: 3359 4418
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7777
BstXI CCAn_nnnn'nTGG
Cuts at: 2076 2590 2076
Size: 514 7263
BstYI r'GATC_y
Cuts at: 1972 2386 3991 4368 5107 5474 5859 5876 7328
Size: 414 1605 377 739 367 385 17 1452
Cuts at: 7328 7414 7426 7772 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1605 1452 739 414 385 377 367
346 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2882 2882
Size: 7777
BtgI C'CryG_G
Cuts at: 513 1065 2004 3002 3997 4363 4743 513
Size: 552 939 998 995 366 380 3547
Fragments arranged by size:
3547 998 995 939 552 380 366
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7038
Fragments arranged by size:
7038 683 56
BtsI GCAGTG_nn'
Cuts at: 2982 3049 4136 4225 4231 4909 4926 5065 5293
Size: 67 1087 89 6 678 17 139 228
Cuts at: 5293 6087 6107 2982
Size: 794 20 4652
Fragments arranged by size:
4652 1087 794 678 228 139 89 67
20 17 6
ClaI AT'CG_AT
Cuts at: 1554 2907 1554
Size: 1353 6424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7742
DraI TTT'AAA
Cuts at: 5081 5081
Size: 7777
DraIII CAC_nnn'GTG
Cuts at: 2234 2234
Size: 7777
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4538 6784 809
Size: 507 3222 2246 1802
Fragments arranged by size:
3222 2246 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2613 2943 3128
Size: 54 1425 50 275 18 783 330 185
Cuts at: 3128 3687 4000 4176 4473 5045 5224 6113 7742
Size: 559 313 176 297 572 179 889 1629
Cuts at: 7742 8
Size: 43
Fragments arranged by size:
1629 1425 889 783 572 559 330 313
297 275 185 179 176 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5224 1537
Size: 275 3412 4090
Fragments arranged by size:
4090 3412 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2266 2627 4349 4553 4806 5707 1353
Size: 913 361 1722 204 253 901 3423
Fragments arranged by size:
3423 1722 913 901 361 253 204
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5083 6364 6738 6884 387
Size: 1064 138 3494 1281 374 146 1280
Fragments arranged by size:
3494 1281 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2298 4441 2298
Size: 2143 5634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 3138 3254 3302 3689 3700 3745
Size: 393 714 792 116 48 387 11 45
Cuts at: 3745 3863 3948 4070 4216 4586 4757 4830 4859
Size: 118 85 122 146 370 171 73 29
Cuts at: 4859 4913 4963 4990 5840 6437 7224 7492 1239
Size: 54 50 27 850 597 787 268 1524
Fragments arranged by size:
1524 850 792 787 714 597 393 387
370 268 171 146 122 118 116 85
73 54 50 48 45 29 27 11
EcoNI CCTnn'n_nnAGG
Cuts at: 2361 2654 4397 4550 4700 5046 2361
Size: 293 1743 153 150 346 5092
Fragments arranged by size:
5092 1743 346 293 153 150
EcoO109I rG'GnC_Cy
Cuts at: 2165 2289 2596 2597 2630 2782 2971 3123 4054
Size: 124 307 1 33 152 189 152 931
Cuts at: 4054 4287 4288 4423 4503 4555 2165
Size: 233 1 135 80 52 5387
Fragments arranged by size:
5387 931 307 233 189 152 152 135
124 80 52 33 1 1
EcoRI G'AATT_C
Cuts at: 2726 5086 5464 2726
Size: 2360 378 5039
Fragments arranged by size:
5039 2360 378
FspI TGC'GCA
Cuts at: 3678 4523 6283 3678
Size: 845 1760 5172
Fragments arranged by size:
5172 1760 845
HaeII r_GCGC'y
Cuts at: 1678 2611 3685 3954 4686 4877 6924 1678
Size: 933 1074 269 732 191 2047 2531
Fragments arranged by size:
2531 2047 1074 933 732 269 191
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2371 2403 2654 2686 3578
Size: 32 135 32 799 32 251 32 892
Cuts at: 3578 3610 3629 3661 6418 6450 6492 6524 1373
Size: 32 19 32 2757 32 42 32 2626
Fragments arranged by size:
2757 2626 892 799 251 135 42 32
32 32 32 32 32 32 32 19
HincII GTy'rAC
Cuts at: 669 1657 1938 5121 5367 669
Size: 988 281 3183 246 3079
Fragments arranged by size:
3183 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7777
HpaI GTT'AAC
Cuts at: 5367 5367
Size: 7777
KasI G'GCGC_C
Cuts at: 3950 3950
Size: 7777
KpnI G_GTAC'C
Cuts at: 3414 5102 3414
Size: 1688 6089
MfeI C'AATT_G
Cuts at: 5376 5376
Size: 7777
MluI A'CGCG_T
Cuts at: 7653 7653
Size: 7777
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2357 2463 2533 3609 6891 7075 1305
Size: 1052 106 70 1076 3282 184 2007
Fragments arranged by size:
3282 2007 1076 1052 184 106 70
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 3689 4475 10
Size: 54 1425 343 783 1074 786 3312
Fragments arranged by size:
3312 1425 1074 786 783 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2062 3809 5735 6094 6253 518
Size: 648 896 1747 1926 359 159 2042
Fragments arranged by size:
2042 1926 1747 896 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2076 3577 4212 4529 4637 4780 4819 5193
Size: 470 1501 635 317 108 143 39 374
Cuts at: 5193 5870 7018 7263 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1501 1148 677 635 470 374 317
245 143 108 39
NarI GG'CG_CC
Cuts at: 3951 3951
Size: 7777
NcoI C'CATG_G
Cuts at: 513 1065 2004 4743 513
Size: 552 939 2739 3547
Fragments arranged by size:
3547 2739 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7777
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7777
NotI GC'GGCC_GC
Cuts at: 5224 5224
Size: 7777
NspI r_CATG'y
Cuts at: 1132 1229 2122 5135 6680 1132
Size: 97 893 3013 1545 2229
Fragments arranged by size:
3013 2229 1545 893 97
PasI CC'CwG_GG
Cuts at: 1348 2011 2250 3488 3625 1348
Size: 663 239 1238 137 5500
Fragments arranged by size:
5500 1238 663 239 137
PciI A'CATG_T
Cuts at: 6676 6676
Size: 7777
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2348 2426 3020 4471 1108
Size: 1240 78 594 1451 4414
Fragments arranged by size:
4414 1451 1240 594 78
PfoI T'CCnGG_A
Cuts at: 3231 4242 4580 3231
Size: 1011 338 6428
Fragments arranged by size:
6428 1011 338
PmeI GTTT'AAAC
Cuts at: 5081 5081
Size: 7777
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5846 5878 7359 7391 5846
Size: 32 1481 32 6232
Fragments arranged by size:
6232 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2165 2630 2165
Size: 465 7312
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7777
PsiI TTA'TAA
Cuts at: 5347 5347
Size: 7777
PspOMI G'GGCC_C
Cuts at: 2289 2596 2971 3123 4287 2289
Size: 307 375 152 1164 5779
Fragments arranged by size:
5779 1164 375 307 152
PspXI vC'TCGA_Gb
Cuts at: 2169 2169
Size: 7777
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3776 3808 3776
Size: 32 7745
PstI C_TGCA'G
Cuts at: 830 4131 4325 4398 4995 5129 830
Size: 3301 194 73 597 134 3478
Fragments arranged by size:
3478 3301 597 194 134 73
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7777
PvuII CAG'CTG
Cuts at: 3577 4529 4637 4780 3577
Size: 952 108 143 6574
Fragments arranged by size:
6574 952 143 108
SacI G_AGCT'C
Cuts at: 2300 4443 2300
Size: 2143 5634
SalI G'TCGA_C
Cuts at: 1936 5119 1936
Size: 3183 4594
SapI GCTCTTCn'nnn_
Cuts at: 4349 4806 4349
Size: 457 7320
SbfI CC_TGCA'GG
Cuts at: 5129 5129
Size: 7777
ScaI AGT'ACT
Cuts at: 2852 6025 7737 2852
Size: 3173 1712 2892
Fragments arranged by size:
3173 2892 1712
SexAI A'CCwGG_T
Cuts at: 2666 2666
Size: 7777
SfcI C'TryA_G
Cuts at: 826 1045 4127 4321 4394 4492 4858 4991 5125
Size: 219 3082 194 73 98 366 133 134
Cuts at: 5125 6260 6941 7132 826
Size: 1135 681 191 1471
Fragments arranged by size:
3082 1471 1135 681 366 219 194 191
134 133 98 73
SfiI GGCCn_nnn'nGGCC
Cuts at: 2942 3731 2942
Size: 789 6988
SfoI GGC'GCC
Cuts at: 3952 3952
Size: 7777
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7777
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7777
SmaI CCC'GGG
Cuts at: 1872 2287 2681 5018 5104 1872
Size: 415 394 2337 86 4545
Fragments arranged by size:
4545 2337 415 394 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2169 2371 2809 3149 3788 4091
Size: 197 926 226 202 438 340 639 303
Cuts at: 4091 4373 4442 5881 6782 7044 820
Size: 282 69 1439 901 262 1553
Fragments arranged by size:
1553 1439 926 901 639 438 340 303
282 262 226 202 197 69
SnaBI TAC'GTA
Cuts at: 493 5568 493
Size: 5075 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7777
SphI G_CATG'C
Cuts at: 1132 5135 1132
Size: 4003 3774
SspI AAT'ATT
Cuts at: 5 52 5701 5
Size: 47 5649 2081
Fragments arranged by size:
5649 2081 47
StuI AGG'CCT
Cuts at: 1517 3113 3287 4097 4832 1517
Size: 1596 174 810 735 4462
Fragments arranged by size:
4462 1596 810 735 174
StyI C'CwwG_G
Cuts at: 513 1065 2004 2425 2742 3288 3434 3529 3550
Size: 552 939 421 317 546 146 95 21
Cuts at: 3550 4079 4191 4743 5216 513
Size: 529 112 552 473 3074
Fragments arranged by size:
3074 939 552 552 546 529 473 421
317 146 112 95 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2281 2381 3851 5804 5821 5974 6159
Size: 1255 701 100 1470 1953 17 153 185
Cuts at: 6159 6604 325
Size: 445 1498
Fragments arranged by size:
1953 1498 1470 1255 701 445 185 153
100 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2204 2524 2850
Size: 274 80 33 51 1443 227 320 326
Cuts at: 2850 4101 4407 6023 7735 96
Size: 1251 306 1616 1712 138
Fragments arranged by size:
1712 1616 1443 1251 326 320 306 274
227 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7737 7769 7737
Size: 32 7745
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7777
XbaI T'CTAG_A
Cuts at: 5113 5113
Size: 7777
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3478 4240 3478
Size: 762 7015
XhoI C'TCGA_G
Cuts at: 1943 2169 1943
Size: 226 7551
XmaI C'CCGG_G
Cuts at: 1870 2285 2679 5016 5102 1870
Size: 415 394 2337 86 4545
Fragments arranged by size:
4545 2337 415 394 86
XmnI GAAnn'nnTTC
Cuts at: 4358 5906 4358
Size: 1548 6229
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5585 276
Size: 53 83 186 936 4051 2468
Fragments arranged by size:
4051 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BaeI BbvCI BfrBI BsiWI BstZ17I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI RsrII SacII SanDI SrfI SwaI