Restriction map for
pFN21AA2010
(Circular) (Six-base) MAPSORT of: pFN21AA2010.seq Check: 2396 from: 1 to: 7165
pFN21AA2010 7165 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2664 4975 278
Size: 53 83 186 936 1128 2311 2468
Fragments arranged by size:
2468 2311 1128 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4508 1937
Size: 2571 4594
Acc65I G'GTAC_C
Cuts at: 4486 4486
Size: 7165
AclI AA'CG_TT
Cuts at: 1563 5292 5665 1563
Size: 3729 373 3063
Fragments arranged by size:
3729 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2244 2376 2664 5228 6612 6880 1632
Size: 497 115 132 288 2564 1384 268 1917
Fragments arranged by size:
2564 1917 1384 497 288 268 132 115
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6968
AflIII A'CryG_T
Cuts at: 1203 2856 6064 7041 1203
Size: 1653 3208 977 1327
Fragments arranged by size:
3208 1653 1327 977
AgeI A'CCGG_T
Cuts at: 5996 5996
Size: 7165
AhdI GACnn_n'nnGTC
Cuts at: 5894 5894
Size: 7165
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7165
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2138 1184
Size: 954 6211
AlwNI CAG_nnn'CTG
Cuts at: 2768 3284 4908 6480 2768
Size: 516 1624 1572 3453
Fragments arranged by size:
3453 1624 1572 516
ApaLI G'TGCA_C
Cuts at: 5222 6378 5222
Size: 1156 6009
ApoI r'AATT_y
Cuts at: 1493 2280 2717 2784 2930 3014 3380 3782 4109
Size: 787 437 67 146 84 366 402 327
Cuts at: 4109 4454 4474 4700 4852 6961 1493
Size: 345 20 226 152 2109 1697
Fragments arranged by size:
2109 1697 787 437 402 366 345 327
226 152 146 84 67 20
AseI AT'TA_AT
Cuts at: 160 1032 3561 5719 160
Size: 872 2529 2158 1606
Fragments arranged by size:
2529 2158 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7165
AvaI C'yCGr_G
Cuts at: 1870 1943 4490 1870
Size: 73 2547 4545
Fragments arranged by size:
4545 2547 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3117 3150 3182 3215 3117
Size: 33 32 33 7067
Fragments arranged by size:
7067 33 33 32
BamHI G'GATC_C
Cuts at: 3737 4096 4283 4310 4495 4862 3737
Size: 359 187 27 185 367 6040
Fragments arranged by size:
6040 367 359 187 185 27
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2072 4486 5941 618
Size: 325 217 627 285 2414 1455 1842
Fragments arranged by size:
2414 1842 1455 627 325 285 217
BanII G_rGCy'C
Cuts at: 1391 4190 4484 1391
Size: 2799 294 4072
Fragments arranged by size:
4072 2799 294
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3456 4229 928
Size: 971 1557 773 3864
Fragments arranged by size:
3864 1557 971 773
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5356 5390 1903
Size: 34 3419 34 3678
Fragments arranged by size:
3678 3419 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5057 6267 5057
Size: 1210 5955
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7165
BfrBI ATG'CAT
Cuts at: 3412 3412
Size: 7165
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5776 136
Size: 107 122 71 631 605 4104 1525
Fragments arranged by size:
4104 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7160 7160
Size: 7165
BlpI GC'TnA_GC
Cuts at: 4582 4582
Size: 7165
Bme1580I G_kGCm'C
Cuts at: 1790 2075 3631 5226 6382 1790
Size: 285 1556 1595 1156 2573
Fragments arranged by size:
2573 1595 1556 1156 285
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7165
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4896 5854 442
Size: 416 53 83 399 24 3479 958 1753
Fragments arranged by size:
3479 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3353 1056
Size: 2297 4868
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 4281 5825 1239
Size: 1307 1735 1544 2579
Fragments arranged by size:
2579 1735 1544 1307
Bpu10I CC'TnA_GC
Cuts at: 2249 2769 2249
Size: 520 6645
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5290 6155 6453 5290
Size: 865 298 6002
Fragments arranged by size:
6002 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5828 882
Size: 617 4329 2219
Fragments arranged by size:
4329 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2098 4956 493
Size: 711 894 2858 2702
Fragments arranged by size:
2858 2702 894 711
BsaBI GATnn'nnATC
Cuts at: 3279 7159 3279
Size: 3880 3285
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2661 4972 5354 5989
Size: 53 83 186 936 1128 2311 382 635
Cuts at: 5989 275
Size: 1451
Fragments arranged by size:
2311 1451 1128 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3576 5598 5996 6270 6417 6828 3576
Size: 2022 398 274 147 411 3913
Fragments arranged by size:
3913 2022 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3285 3315 4035 4065 4350
Size: 30 165 30 1882 30 720 30 285
Cuts at: 4350 4380 1178
Size: 30 3963
Fragments arranged by size:
3963 1882 720 285 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 3309 1186
Size: 1189 934 5042
Fragments arranged by size:
5042 1189 934
BseYI C'CCAG_C
Cuts at: 6368 6864 6368
Size: 496 6669
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2555 1163
Size: 573 819 5773
Fragments arranged by size:
5773 819 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2068 4615 5376 6404 1540
Size: 275 186 67 2547 761 1028 2301
Fragments arranged by size:
2547 2301 1028 761 275 186 67
BsiHKAI G_wGCw'C
Cuts at: 3531 5226 5311 6382 3531
Size: 1695 85 1071 4314
Fragments arranged by size:
4314 1695 1071 85
BsmI GAATG_Cn'
Cuts at: 4676 4769 4676
Size: 93 7072
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 3531 3631 4190 4484 5226 5311
Size: 399 285 1456 100 559 294 742 85
Cuts at: 5311 6382 1391
Size: 1071 2174
Fragments arranged by size:
2174 1456 1071 742 559 399 294 285
100 85
BspEI T'CCGG_A
Cuts at: 3576 6828 3576
Size: 3252 3913
BspHI T'CATG_A
Cuts at: 5054 6784 5054
Size: 1730 5435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4520 844
Size: 3676 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2104 5052 1394
Size: 429 281 2948 3507
Fragments arranged by size:
3507 2948 429 281
BsrDI GCAATG_nn'
Cuts at: 65 1248 5660 5834 65
Size: 1183 4412 174 1396
Fragments arranged by size:
4412 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2052 5809 5996 1700
Size: 110 242 3757 187 2869
Fragments arranged by size:
3757 2869 242 187 110
BsrGI T'GTAC_A
Cuts at: 96 2034 3865 96
Size: 1938 1831 3396
Fragments arranged by size:
3396 1938 1831
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7165
BssSI C'ACGA_G
Cuts at: 4364 5225 6237 4364
Size: 861 1012 5292
Fragments arranged by size:
5292 1012 861
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3409 3626 794
Size: 2615 217 4333
Fragments arranged by size:
4333 2615 217
BstBI TT'CG_AA
Cuts at: 3322 5969 3322
Size: 2647 4518
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7165
BstYI r'GATC_y
Cuts at: 1972 2764 3280 3737 4096 4283 4292 4310 4495
Size: 792 516 457 359 187 9 18 185
Cuts at: 4495 4862 5247 5264 6716 6802 6814 7160 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 792 516 457 385 367 359
346 187 185 86 18 17 12 9
BtgI C'CryG_G
Cuts at: 513 1065 2322 3186 513
Size: 552 1257 864 4492
Fragments arranged by size:
4492 1257 864 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2367 487
Size: 683 56 1141 5285
Fragments arranged by size:
5285 1141 683 56
BtsI GCAGTG_nn'
Cuts at: 2063 2181 3354 3420 4681 5475 5495 2063
Size: 118 1173 66 1261 794 20 3733
Fragments arranged by size:
3733 1261 1173 794 118 66 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7165
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7130
DraI TTT'AAA
Cuts at: 2743 3009 3640 3877 4469 2743
Size: 266 631 237 592 5439
Fragments arranged by size:
5439 631 592 266 237
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6172 809
Size: 507 4856 1802
Fragments arranged by size:
4856 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4612 5501 7130
Size: 54 1425 50 275 18 2782 889 1629
Cuts at: 7130 8
Size: 43
Fragments arranged by size:
2782 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4612 1537
Size: 275 2800 4090
Fragments arranged by size:
4090 2800 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2352 2472 3967 3996 5095 1353
Size: 999 120 1495 29 1099 3423
Fragments arranged by size:
3423 1495 1099 999 120 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5752 6126 6272 387
Size: 1064 138 4163 374 146 1280
Fragments arranged by size:
4163 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2244 2376 2546 2664 4281 5228
Size: 393 497 115 132 170 118 1617 947
Cuts at: 5228 5825 6612 6880 1239
Size: 597 787 268 1524
Fragments arranged by size:
1617 1524 947 787 597 497 393 268
170 132 118 115
EcoO109I rG'GnC_Cy
Cuts at: 2314 2338 2314
Size: 24 7141
EcoRI G'AATT_C
Cuts at: 4474 4852 4474
Size: 378 6787
EcoRV GAT'ATC
Cuts at: 3571 3968 3571
Size: 397 6768
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3128 3128
Size: 7165
FspI TGC'GCA
Cuts at: 5671 5671
Size: 7165
HaeII r_GCGC'y
Cuts at: 1678 6312 1678
Size: 4634 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2363 2366 2395 2398 3143
Size: 32 135 32 791 3 29 3 745
Cuts at: 3143 3175 3186 3218 3285 3317 3791 3823 5806
Size: 32 11 32 67 32 474 32 1983
Cuts at: 5806 5838 5880 5912 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1983 791 745 474 135 67 42
32 32 32 32 32 32 32 32
29 11 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 3001 3334 3513 4509 4755 669
Size: 988 281 1063 333 179 996 246 3079
Fragments arranged by size:
3079 1063 996 988 333 281 246 179
HindIII A'AGCT_T
Cuts at: 748 4199 748
Size: 3451 3714
HpaI GTT'AAC
Cuts at: 3001 3513 4755 3001
Size: 512 1242 5411
Fragments arranged by size:
5411 1242 512
KpnI G_GTAC'C
Cuts at: 4490 4490
Size: 7165
MfeI C'AATT_G
Cuts at: 4764 4764
Size: 7165
MluI A'CGCG_T
Cuts at: 7041 7041
Size: 7165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6279 6463 1305
Size: 4974 184 2007
Fragments arranged by size:
4974 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5343
Fragments arranged by size:
5343 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2541 2580 5123 5482 5641 518
Size: 648 1375 39 2543 359 159 2042
Fragments arranged by size:
2543 2042 1375 648 359 159 39
MspA1I CmG'CkG
Cuts at: 1606 4581 5258 6406 6651 1606
Size: 2975 677 1148 245 2120
Fragments arranged by size:
2975 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3186 513
Size: 552 2121 4492
Fragments arranged by size:
4492 2121 552
NdeI CA'TA_TG
Cuts at: 387 3264 387
Size: 2877 4288
NheI G'CTAG_C
Cuts at: 1052 3349 1052
Size: 2297 4868
NotI GC'GGCC_GC
Cuts at: 4612 4612
Size: 7165
NsiI A_TGCA'T
Cuts at: 3414 3414
Size: 7165
NspI r_CATG'y
Cuts at: 1132 1229 2084 2119 2860 2914 3349 3416 3611
Size: 97 855 35 741 54 435 67 195
Cuts at: 3611 4523 6068 1132
Size: 912 1545 2229
Fragments arranged by size:
2229 1545 912 855 741 435 195 97
67 54 35
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7165
PciI A'CATG_T
Cuts at: 2856 6064 2856
Size: 3208 3957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3310 3490 1108
Size: 2202 180 4783
Fragments arranged by size:
4783 2202 180
PfoI T'CCnGG_A
Cuts at: 2523 4270 4306 2523
Size: 1747 36 5382
Fragments arranged by size:
5382 1747 36
PmeI GTTT'AAAC
Cuts at: 4469 4469
Size: 7165
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2131 2163 3573 3605 5234 5266 6747 6779 2131
Size: 32 1410 32 1629 32 1481 32 2517
Fragments arranged by size:
2517 1629 1481 1410 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2314 2338 2314
Size: 24 7141
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7165
PsiI TTA'TAA
Cuts at: 4735 4735
Size: 7165
PstI C_TGCA'G
Cuts at: 830 4517 830
Size: 3687 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7165
SalI G'TCGA_C
Cuts at: 1936 4507 1936
Size: 2571 4594
SbfI CC_TGCA'GG
Cuts at: 4517 4517
Size: 7165
ScaI AGT'ACT
Cuts at: 5413 7125 5413
Size: 1712 5453
SfcI C'TryA_G
Cuts at: 826 1045 3038 4050 4513 5648 6329 6520 826
Size: 219 1993 1012 463 1135 681 191 1471
Fragments arranged by size:
1993 1471 1135 1012 681 463 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7165
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7165
SmaI CCC'GGG
Cuts at: 1872 4492 1872
Size: 2620 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5269 6170 6432 820
Size: 197 926 3326 901 262 1553
Fragments arranged by size:
3326 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4956 493
Size: 4463 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7165
SphI G_CATG'C
Cuts at: 1132 3611 4523 1132
Size: 2479 912 3774
Fragments arranged by size:
3774 2479 912
SspI AAT'ATT
Cuts at: 5 52 2560 5089 5
Size: 47 2508 2529 2081
Fragments arranged by size:
2529 2508 2081 47
StuI AGG'CCT
Cuts at: 1517 4362 1517
Size: 2845 4320
StyI C'CwwG_G
Cuts at: 513 1065 3112 3186 4604 513
Size: 552 2047 74 1418 3074
Fragments arranged by size:
3074 2047 1418 552 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 2830 5192 5209 5362 5547 5992
Size: 1255 444 806 2362 17 153 185 445
Cuts at: 5992 325
Size: 1498
Fragments arranged by size:
2362 1498 1255 806 445 444 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2034 3865 5411
Size: 274 80 33 51 1443 57 1831 1546
Cuts at: 5411 7123 96
Size: 1712 138
Fragments arranged by size:
1831 1712 1546 1443 274 138 80 57
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2042 2074 4350 4382 7125 7157 2042
Size: 32 2276 32 2743 32 2050
Fragments arranged by size:
2743 2276 2050 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3073 1316
Size: 1757 5408
XbaI T'CTAG_A
Cuts at: 4501 4501
Size: 7165
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7165
XmaI C'CCGG_G
Cuts at: 1870 4490 1870
Size: 2620 4545
XmnI GAAnn'nnTTC
Cuts at: 2751 2931 4155 5294 2751
Size: 180 1224 1139 4622
Fragments arranged by size:
4622 1224 1139 180
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2662 4973 276
Size: 53 83 186 936 1128 2311 2468
Fragments arranged by size:
2468 2311 1128 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI