Restriction map for
pFN21AA2009
(Circular) (Six-base) MAPSORT of: pFN21AA2009.seq Check: 9711 from: 1 to: 6412
pFN21AA2009 6412 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4222 278
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3755 1937
Size: 1818 4594
Acc65I G'GTAC_C
Cuts at: 2503 3733 2503
Size: 1230 5182
AclI AA'CG_TT
Cuts at: 1563 4539 4912 1563
Size: 2976 373 3063
Fragments arranged by size:
3063 2976 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4475 5859 6127 1632
Size: 2843 1384 268 1917
Fragments arranged by size:
2843 1917 1384 268
AfeI AGC'GCT
Cuts at: 2277 2277
Size: 6412
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6215
AflIII A'CryG_T
Cuts at: 1203 2577 3471 5311 6288 1203
Size: 1374 894 1840 977 1327
Fragments arranged by size:
1840 1374 1327 977 894
AgeI A'CCGG_T
Cuts at: 5243 5243
Size: 6412
AhdI GACnn_n'nnGTC
Cuts at: 5141 5141
Size: 6412
AleI CACnn'nnGTG
Cuts at: 1166 2744 1166
Size: 1578 4834
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6412
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2600 2632 2600
Size: 32 6380
AlwNI CAG_nnn'CTG
Cuts at: 2796 2947 4155 5727 2796
Size: 151 1208 1572 3481
Fragments arranged by size:
3481 1572 1208 151
ApaI G_GGCC'C
Cuts at: 2538 3139 2538
Size: 601 5811
ApaLI G'TGCA_C
Cuts at: 3287 4469 5625 3287
Size: 1182 1156 4074
Fragments arranged by size:
4074 1182 1156
ApoI r'AATT_y
Cuts at: 1493 3721 3947 4099 6208 1493
Size: 2228 226 152 2109 1697
Fragments arranged by size:
2228 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4966 160
Size: 872 3934 1606
Fragments arranged by size:
3934 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
AvaI C'yCGr_G
Cuts at: 1870 1943 2477 2655 3074 3154 3239 3349 3394
Size: 73 534 178 419 80 85 110 45
Cuts at: 3394 3659 3737 1870
Size: 265 78 4545
Fragments arranged by size:
4545 534 419 265 178 110 85 80
78 73 45
BamHI G'GATC_C
Cuts at: 3742 4109 3742
Size: 367 6045
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2455 2503 2543 2738
Size: 325 217 627 369 299 48 40 195
Cuts at: 2738 3121 3248 3308 3595 3733 5188 618
Size: 383 127 60 287 138 1455 1842
Fragments arranged by size:
1842 1455 627 383 369 325 299 287
217 195 138 127 60 48 40
BanII G_rGCy'C
Cuts at: 1391 2104 2526 2538 2709 3139 3482 3660 3731
Size: 713 422 12 171 430 343 178 71
Cuts at: 3731 1391
Size: 4072
Fragments arranged by size:
4072 713 430 422 343 178 171 71
12
BbeI G_GCGC'C
Cuts at: 2160 2547 3252 2160
Size: 387 705 5320
Fragments arranged by size:
5320 705 387
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2294 3226 928
Size: 971 395 932 4114
Fragments arranged by size:
4114 971 932 395
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3566 3600 4603 4637 1903
Size: 34 50 34 1545 34 1003 34 3678
Fragments arranged by size:
3678 1545 1003 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4304 5514 4304
Size: 1210 5202
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6412
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3353 5023 136
Size: 107 122 71 631 605 1681 1670 1525
Fragments arranged by size:
1681 1670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6407 6407
Size: 6412
BlpI GC'TnA_GC
Cuts at: 3829 3829
Size: 6412
Bme1580I G_kGCm'C
Cuts at: 1790 2538 3124 3139 3291 3445 3600 3668 4473
Size: 748 586 15 152 154 155 68 805
Cuts at: 4473 5629 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 805 748 586 155 154 152
68 15
BmgBI CAC'GTC
Cuts at: 1318 3474 1318
Size: 2156 4256
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2217 2306 3133
Size: 416 53 83 399 24 800 89 827
Cuts at: 3133 4143 5101 442
Size: 1010 958 1753
Fragments arranged by size:
1753 1010 958 827 800 416 399 89
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6412
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2176 2404 2422 2176
Size: 228 18 6166
Fragments arranged by size:
6166 228 18
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2818 2854 3490 5072 1239
Size: 812 767 36 636 1582 2579
Fragments arranged by size:
2579 1582 812 767 636 36
Bpu10I CC'TnA_GC
Cuts at: 2978 2978
Size: 6412
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4537 5402 5700 4537
Size: 865 298 5249
Fragments arranged by size:
5249 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2075 5075 882
Size: 617 576 3000 2219
Fragments arranged by size:
3000 2219 617 576
BsaAI yAC'GTr
Cuts at: 493 1204 2580 4203 493
Size: 711 1376 1623 2702
Fragments arranged by size:
2702 1623 1376 711
BsaBI GATnn'nnATC
Cuts at: 2760 6406 2760
Size: 3646 2766
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 2544 2591 3249
Size: 53 83 186 936 624 387 47 658
Cuts at: 3249 4219 4601 5236 275
Size: 970 382 635 1451
Fragments arranged by size:
1451 970 936 658 635 624 387 382
186 83 53 47
BsaWI w'CCGG_w
Cuts at: 3175 4845 5243 5517 5664 6075 3175
Size: 1670 398 274 147 411 3512
Fragments arranged by size:
3512 1670 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 2600 2630 2852
Size: 30 165 30 708 30 459 30 222
Cuts at: 2852 2882 1178
Size: 30 4708
Fragments arranged by size:
4708 708 459 222 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2675 3214 3417 3472 3475 3490 3493
Size: 1153 336 539 203 55 3 15 3
Cuts at: 3493 1186
Size: 4105
Fragments arranged by size:
4105 1153 539 336 203 55 15 3
3
BseYI C'CCAG_C
Cuts at: 2009 2134 2594 2813 2940 3436 5615 6111 2009
Size: 125 460 219 127 496 2179 496 2310
Fragments arranged by size:
2310 2179 496 496 460 219 127 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3284 1163
Size: 573 1548 4291
Fragments arranged by size:
4291 1548 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2836 3862 4623 5651 1540
Size: 275 186 835 1026 761 1028 2301
Fragments arranged by size:
2301 1028 1026 835 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 2709 3291 3366 4473 4558 5629 2403
Size: 306 582 75 1107 85 1071 3186
Fragments arranged by size:
3186 1107 1071 582 306 85 75
BsmI GAATG_Cn'
Cuts at: 3923 4016 3923
Size: 93 6319
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2104 2403 2526 2538 2709 3124 3139
Size: 399 314 299 123 12 171 415 15
Cuts at: 3139 3291 3366 3445 3482 3600 3660 3668 3731
Size: 152 75 79 37 118 60 8 63
Cuts at: 3731 4473 4558 5629 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 415 399 314 299 171
152 123 118 85 79 75 63 60
37 15 12 8
BspEI T'CCGG_A
Cuts at: 6075 6075
Size: 6412
BspHI T'CATG_A
Cuts at: 4301 6031 4301
Size: 1730 4682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2481 3175 3767 844
Size: 1637 694 592 3489
Fragments arranged by size:
3489 1637 694 592
BsrBI CCG'CTC
Cuts at: 1394 1823 3107 3148 4299 1394
Size: 429 1284 41 1151 3507
Fragments arranged by size:
3507 1284 1151 429 41
BsrDI GCAATG_nn'
Cuts at: 65 1248 2676 3022 4907 5081 65
Size: 1183 1428 346 1885 174 1396
Fragments arranged by size:
1885 1428 1396 1183 346 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2611 2627 2690 3294 5056 5243 1700
Size: 110 801 16 63 604 1762 187 2869
Fragments arranged by size:
2869 1762 801 604 187 110 63 16
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6412
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6412
BssSI C'ACGA_G
Cuts at: 2525 4472 5484 2525
Size: 1947 1012 3453
Fragments arranged by size:
3453 1947 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3315 3627 794
Size: 2521 312 3579
Fragments arranged by size:
3579 2521 312
BstBI TT'CG_AA
Cuts at: 5216 5216
Size: 6412
BstEII G'GTnAC_C
Cuts at: 1184 2623 1184
Size: 1439 4973
BstXI CCAn_nnnn'nTGG
Cuts at: 3132 3436 3132
Size: 304 6108
BstYI r'GATC_y
Cuts at: 1972 3742 4109 4494 4511 5963 6049 6061 6407
Size: 1770 367 385 17 1452 86 12 346
Cuts at: 6407 1972
Size: 1977
Fragments arranged by size:
1977 1770 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2367 3228 3681 513
Size: 552 1302 861 453 3244
Fragments arranged by size:
3244 1302 861 552 453
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5673
Fragments arranged by size:
5673 683 56
BtsI GCAGTG_nn'
Cuts at: 2890 3928 4722 4742 2890
Size: 1038 794 20 4560
Fragments arranged by size:
4560 1038 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6412
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6377
DraI TTT'AAA
Cuts at: 3716 3716
Size: 6412
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5419 809
Size: 507 4103 1802
Fragments arranged by size:
4103 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 2833 3604
Size: 54 1425 50 275 18 534 469 771
Cuts at: 3604 3859 4748 6377 8
Size: 255 889 1629 43
Fragments arranged by size:
1629 1425 889 771 534 469 275 255
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2833 3859 1537
Size: 275 1021 1026 4090
Fragments arranged by size:
4090 1026 1021 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2183 3199 3493 3517 3526 3620 3644 4342
Size: 830 1016 294 24 9 94 24 698
Cuts at: 4342 1353
Size: 3423
Fragments arranged by size:
3423 1016 830 698 294 94 24 24
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2966 3177 4999 5373 5519 387
Size: 1064 138 1377 211 1822 374 146 1280
Fragments arranged by size:
1822 1377 1280 1064 374 211 146 138
EcoICRI GAG'CTC
Cuts at: 2707 2707
Size: 6412
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2818 2854 3490 4475 5072 5859
Size: 393 419 767 36 636 985 597 787
Cuts at: 5859 6127 1239
Size: 268 1524
Fragments arranged by size:
1524 985 787 767 636 597 419 393
268 36
EcoO109I rG'GnC_Cy
Cuts at: 2534 2534
Size: 6412
EcoRI G'AATT_C
Cuts at: 3721 4099 3721
Size: 378 6034
FspI TGC'GCA
Cuts at: 2988 3386 4918 2988
Size: 398 1532 4482
Fragments arranged by size:
4482 1532 398
HaeII r_GCGC'y
Cuts at: 1678 2160 2279 2547 2947 3252 5559 1678
Size: 482 119 268 400 305 2307 2531
Fragments arranged by size:
2531 2307 482 400 305 268 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2111 2143 5053 5085 5127
Size: 32 135 32 539 32 2910 32 42
Cuts at: 5127 5159 1373
Size: 32 2626
Fragments arranged by size:
2910 2626 539 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3756 4002 669
Size: 988 281 1818 246 3079
Fragments arranged by size:
3079 1818 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6412
HpaI GTT'AAC
Cuts at: 4002 4002
Size: 6412
KasI G'GCGC_C
Cuts at: 2156 2543 3248 2156
Size: 387 705 5320
Fragments arranged by size:
5320 705 387
KpnI G_GTAC'C
Cuts at: 2507 3737 2507
Size: 1230 5182
MfeI C'AATT_G
Cuts at: 4011 4011
Size: 6412
MluI A'CGCG_T
Cuts at: 6288 6288
Size: 6412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3288 3447 5526 5710 1305
Size: 1983 159 2079 184 2007
Fragments arranged by size:
2079 2007 1983 184 159
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2366 3606 10
Size: 54 1425 343 534 1240 2816
Fragments arranged by size:
2816 1425 1240 534 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2495 2744 4370 4729 4888 518
Size: 648 1329 249 1626 359 159 2042
Fragments arranged by size:
2042 1626 1329 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2046 2070 2933 3035 3315 3448 3683 3828
Size: 440 24 863 102 280 133 235 145
Cuts at: 3828 4505 5653 5898 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 863 677 440 280 245 235
145 133 102 24
NaeI GCC'GGC
Cuts at: 3296 3296
Size: 6412
NarI GG'CG_CC
Cuts at: 2157 2544 3249 2157
Size: 387 705 5320
Fragments arranged by size:
5320 705 387
NcoI C'CATG_G
Cuts at: 513 1065 2367 513
Size: 552 1302 4558
Fragments arranged by size:
4558 1302 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6412
NgoMIV G'CCGG_C
Cuts at: 3294 3294
Size: 6412
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6412
NotI GC'GGCC_GC
Cuts at: 3859 3859
Size: 6412
NspI r_CATG'y
Cuts at: 1132 1229 2237 2635 3770 5315 1132
Size: 97 1008 398 1135 1545 2229
Fragments arranged by size:
2229 1545 1135 1008 398 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6412
PciI A'CATG_T
Cuts at: 5311 5311
Size: 6412
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2947 1108
Size: 1839 4573
PfoI T'CCnGG_A
Cuts at: 2423 2423
Size: 6412
PmeI GTTT'AAAC
Cuts at: 3716 3716
Size: 6412
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2600 2632 4481 4513 5994 6026 2600
Size: 32 1849 32 1481 32 2986
Fragments arranged by size:
2986 1849 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6412
PsiI TTA'TAA
Cuts at: 3982 3982
Size: 6412
PspOMI G'GGCC_C
Cuts at: 2534 3135 2534
Size: 601 5811
PstI C_TGCA'G
Cuts at: 830 2109 3084 3172 3764 830
Size: 1279 975 88 592 3478
Fragments arranged by size:
3478 1279 975 592 88
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6412
PvuII CAG'CTG
Cuts at: 3315 3448 3315
Size: 133 6279
SacI G_AGCT'C
Cuts at: 2709 2709
Size: 6412
SacII CC_GC'GG
Cuts at: 3684 3684
Size: 6412
SalI G'TCGA_C
Cuts at: 1936 3754 1936
Size: 1818 4594
SapI GCTCTTCn'nnn_
Cuts at: 2183 3199 3517 3644 2183
Size: 1016 318 127 4951
Fragments arranged by size:
4951 1016 318 127
SbfI CC_TGCA'GG
Cuts at: 3764 3764
Size: 6412
ScaI AGT'ACT
Cuts at: 4660 6372 4660
Size: 1712 4700
SfcI C'TryA_G
Cuts at: 826 1045 2105 2956 3080 3168 3760 4895 5576
Size: 219 1060 851 124 88 592 1135 681
Cuts at: 5576 5767 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1060 851 681 592 219 191
124 88
SfoI GGC'GCC
Cuts at: 2158 2545 3250 2158
Size: 387 705 5320
Fragments arranged by size:
5320 705 387
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6412
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6412
SmaI CCC'GGG
Cuts at: 1872 2479 3156 3241 3351 3396 3739 1872
Size: 607 677 85 110 45 343 4545
Fragments arranged by size:
4545 677 607 343 110 85 45
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2655 4516 5417 5679 820
Size: 197 926 712 1861 901 262 1553
Fragments arranged by size:
1861 1553 926 901 712 262 197
SnaBI TAC'GTA
Cuts at: 493 4203 493
Size: 3710 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6412
SphI G_CATG'C
Cuts at: 1132 2635 3770 1132
Size: 1503 1135 3774
Fragments arranged by size:
3774 1503 1135
SrfI GCCC'GGGC
Cuts at: 2479 2479
Size: 6412
SspI AAT'ATT
Cuts at: 5 52 4336 5
Size: 47 4284 2081
Fragments arranged by size:
4284 2081 47
StuI AGG'CCT
Cuts at: 1517 3408 1517
Size: 1891 4521
StyI C'CwwG_G
Cuts at: 513 1065 2367 3851 513
Size: 552 1302 1484 3074
Fragments arranged by size:
3074 1484 1302 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2219 4439 4456 4609 4794 5239 325
Size: 1255 639 2220 17 153 185 445 1498
Fragments arranged by size:
2220 1498 1255 639 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4658 6370 96
Size: 274 80 33 51 1443 2681 1712 138
Fragments arranged by size:
2681 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6372 6404 6372
Size: 32 6380
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6412
XbaI T'CTAG_A
Cuts at: 3748 3748
Size: 6412
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2793 3271 2793
Size: 478 5934
XhoI C'TCGA_G
Cuts at: 1943 2655 1943
Size: 712 5700
XmaI C'CCGG_G
Cuts at: 1870 2477 3154 3239 3349 3394 3737 1870
Size: 607 677 85 110 45 343 4545
Fragments arranged by size:
4545 677 607 343 110 85 45
XmnI GAAnn'nnTTC
Cuts at: 2723 4541 2723
Size: 1818 4594
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4220 276
Size: 53 83 186 936 2686 2468
Fragments arranged by size:
2686 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SanDI SexAI SfiI SwaI