Restriction map for
pFN21AA2004
(Circular) (Six-base) MAPSORT of: pFN21AA2004.seq Check: 4132 from: 1 to: 9472
pFN21AA2004 9472 bp
With 182 enzymes: SgfI *
October 20, 2009 10:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7282 278
Size: 53 83 186 936 5746 2468
Fragments arranged by size:
5746 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2545 5382 6815 1937
Size: 608 2837 1433 4594
Fragments arranged by size:
4594 2837 1433 608
Acc65I G'GTAC_C
Cuts at: 6793 6793
Size: 9472
AclI AA'CG_TT
Cuts at: 1563 7599 7972 1563
Size: 6036 373 3063
Fragments arranged by size:
6036 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2658 3317 7535 8919 9187 1632
Size: 1026 659 4218 1384 268 1917
Fragments arranged by size:
4218 1917 1384 1026 659 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9275
AflIII A'CryG_T
Cuts at: 1203 2483 8371 9348 1203
Size: 1280 5888 977 1327
Fragments arranged by size:
5888 1327 1280 977
AgeI A'CCGG_T
Cuts at: 8303 8303
Size: 9472
AhdI GACnn_n'nnGTC
Cuts at: 8201 8201
Size: 9472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 9472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2866 5272 6453 1184
Size: 1682 2406 1181 4203
Fragments arranged by size:
4203 2406 1682 1181
AlwNI CAG_nnn'CTG
Cuts at: 7215 8787 7215
Size: 1572 7900
ApaLI G'TGCA_C
Cuts at: 3737 7529 8685 3737
Size: 3792 1156 4524
Fragments arranged by size:
4524 3792 1156
ApoI r'AATT_y
Cuts at: 1493 2106 2507 2591 2645 2686 3017 3140 3804
Size: 613 401 84 54 41 331 123 664
Cuts at: 3804 4016 4025 4274 4823 4868 5047 5081 5631
Size: 212 9 249 549 45 179 34 550
Cuts at: 5631 5786 6163 6388 6781 7007 7159 9268 1493
Size: 155 377 225 393 226 152 2109 1697
Fragments arranged by size:
2109 1697 664 613 550 549 401 393
377 331 249 226 225 212 179 155
152 123 84 54 45 41 34 9
AseI AT'TA_AT
Cuts at: 160 1032 3785 6528 8026 160
Size: 872 2753 2743 1498 1606
Fragments arranged by size:
2753 2743 1606 1498 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9472
AvaI C'yCGr_G
Cuts at: 1870 1943 6797 1870
Size: 73 4854 4545
Fragments arranged by size:
4854 4545 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2472 2505 2472
Size: 33 9439
BamHI G'GATC_C
Cuts at: 4542 5725 6802 7169 4542
Size: 1183 1077 367 6845
Fragments arranged by size:
6845 1183 1077 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4886 6793 8248 618
Size: 325 217 627 3099 1907 1455 1842
Fragments arranged by size:
3099 1907 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2430 6045 6791 1391
Size: 1039 3615 746 4072
Fragments arranged by size:
4072 3615 1039 746
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2829 3603 4053 4881 6521 928
Size: 971 930 774 450 828 1640 3879
Fragments arranged by size:
3879 1640 971 930 828 774 450
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7663 7697 1903
Size: 34 5726 34 3678
Fragments arranged by size:
5726 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5237 5559 6206 7364 8574 5237
Size: 322 647 1158 1210 6135
Fragments arranged by size:
6135 1210 1158 647 322
BclI T'GATC_A
Cuts at: 1548 4662 1548
Size: 3114 6358
BfrBI ATG'CAT
Cuts at: 5249 6418 5249
Size: 1169 8303
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 8083 136
Size: 107 122 71 631 605 6411 1525
Fragments arranged by size:
6411 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3766 9467 3766
Size: 5701 3771
BlpI GC'TnA_GC
Cuts at: 6889 6889
Size: 9472
Bme1580I G_kGCm'C
Cuts at: 1790 3741 4889 7533 8689 1790
Size: 1951 1148 2644 1156 2573
Fragments arranged by size:
2644 2573 1951 1156 1148
BmgBI CAC'GTC
Cuts at: 1318 2125 1318
Size: 807 8665
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2271 5326 5381
Size: 416 53 83 399 24 854 3055 55
Cuts at: 5381 7203 8161 442
Size: 1822 958 1753
Fragments arranged by size:
3055 1822 1753 958 854 416 399 83
55 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3686 3686
Size: 9472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5621 6193 8132 1239
Size: 4382 572 1939 2579
Fragments arranged by size:
4382 2579 1939 572
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5467 6360 7597 8462 8760 5467
Size: 893 1237 865 298 6179
Fragments arranged by size:
6179 1237 893 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 8135 882
Size: 617 6636 2219
Fragments arranged by size:
6636 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2693 4378 7263 493
Size: 711 1489 1685 2885 2702
Fragments arranged by size:
2885 2702 1685 1489 711
BsaBI GATnn'nnATC
Cuts at: 2061 9466 2061
Size: 7405 2067
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 7279 7661 8296 275
Size: 53 83 186 936 5746 382 635 1451
Fragments arranged by size:
5746 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4707 7905 8303 8577 8724 9135 4707
Size: 3198 398 274 147 411 5044
Fragments arranged by size:
5044 3198 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4317 4347 1178
Size: 30 165 30 2914 30 6303
Fragments arranged by size:
6303 2914 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 9472
BseYI C'CCAG_C
Cuts at: 3470 8675 9171 3470
Size: 5205 496 3771
Fragments arranged by size:
5205 3771 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4188 1163
Size: 573 2452 6447
Fragments arranged by size:
6447 2452 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6922 7683 8711 1540
Size: 275 186 4921 761 1028 2301
Fragments arranged by size:
4921 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2430 3741 7533 7618 8689 2430
Size: 1311 3792 85 1071 3213
Fragments arranged by size:
3792 3213 1311 1071 85
BsmI GAATG_Cn'
Cuts at: 2853 2954 5249 5489 6983 7076 2853
Size: 101 2295 240 1494 93 5249
Fragments arranged by size:
5249 2295 1494 240 101 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3431 3431
Size: 9472
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2430 3741 4889 6045 6791 7533 7618
Size: 399 640 1311 1148 1156 746 742 85
Cuts at: 7618 8689 1391
Size: 1071 2174
Fragments arranged by size:
2174 1311 1156 1148 1071 746 742 640
399 85
BspEI T'CCGG_A
Cuts at: 4707 9135 4707
Size: 4428 5044
BspHI T'CATG_A
Cuts at: 3948 4665 7361 9091 3948
Size: 717 2696 1730 4329
Fragments arranged by size:
4329 2696 1730 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3483 6827 844
Size: 2639 3344 3489
Fragments arranged by size:
3489 3344 2639
BsrBI CCG'CTC
Cuts at: 1394 1823 7359 1394
Size: 429 5536 3507
Fragments arranged by size:
5536 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2391 2659 3493 4158 4365 5311 7967
Size: 1183 1143 268 834 665 207 946 2656
Cuts at: 7967 8141 65
Size: 174 1396
Fragments arranged by size:
2656 1396 1183 1143 946 834 665 268
207 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8116 8303 1700
Size: 110 6306 187 2869
Fragments arranged by size:
6306 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9472
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9472
BssSI C'ACGA_G
Cuts at: 7532 8544 7532
Size: 1012 8460
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5095 5477 794
Size: 4301 382 4789
Fragments arranged by size:
4789 4301 382
BstBI TT'CG_AA
Cuts at: 8276 8276
Size: 9472
BstEII G'GTnAC_C
Cuts at: 1184 4333 1184
Size: 3149 6323
BstXI CCAn_nnnn'nTGG
Cuts at: 3540 3540
Size: 9472
BstYI r'GATC_y
Cuts at: 1972 3664 3700 3766 4542 5725 6802 7169 7554
Size: 1692 36 66 776 1183 1077 367 385
Cuts at: 7554 7571 9023 9109 9121 9467 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1692 1452 1183 1077 776 385 367
346 86 66 36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2734 513
Size: 552 939 730 7251
Fragments arranged by size:
7251 939 730 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5139 487
Size: 683 56 3913 4820
Fragments arranged by size:
4820 3913 683 56
BtsI GCAGTG_nn'
Cuts at: 6988 7782 7802 6988
Size: 794 20 8658
Fragments arranged by size:
8658 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2853 2888 529
Size: 35 2289 35 7113
Fragments arranged by size:
7113 2289 35 35
DraI TTT'AAA
Cuts at: 3091 3103 6021 6776 3091
Size: 12 2918 755 5787
Fragments arranged by size:
5787 2918 755 12
DraIII CAC_nnn'GTG
Cuts at: 4817 4817
Size: 9472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2831 8479 809
Size: 507 1515 5648 1802
Fragments arranged by size:
5648 1802 1515 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 6174 6323 6919
Size: 54 1425 50 275 18 4344 149 596
Cuts at: 6919 7808 9437 8
Size: 889 1629 43
Fragments arranged by size:
4344 1629 1425 889 596 275 149 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6919 1537
Size: 275 5107 4090
Fragments arranged by size:
5107 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2622 3834 4099 4776 5862 7402 1353
Size: 1269 1212 265 677 1086 1540 3423
Fragments arranged by size:
3423 1540 1269 1212 1086 677 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 8059 8433 8579 387
Size: 1064 138 6470 374 146 1280
Fragments arranged by size:
6470 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2428 2428
Size: 9472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2658 3317 5621 6193 7535 8132 8919
Size: 393 1026 659 2304 572 1342 597 787
Cuts at: 8919 9187 1239
Size: 268 1524
Fragments arranged by size:
2304 1524 1342 1026 787 659 597 572
393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2556 2556
Size: 9472
EcoRI G'AATT_C
Cuts at: 2507 2591 2686 4025 6781 7159 2507
Size: 84 95 1339 2756 378 4820
Fragments arranged by size:
4820 2756 1339 378 95 84
EcoRV GAT'ATC
Cuts at: 3946 3946
Size: 9472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2180 3788 4125 5314 5633 6263 2180
Size: 1608 337 1189 319 630 5389
Fragments arranged by size:
5389 1608 1189 630 337 319
FspI TGC'GCA
Cuts at: 7978 7978
Size: 9472
HaeII r_GCGC'y
Cuts at: 1678 8619 1678
Size: 6941 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3190 3222 3323 3355 3433
Size: 32 135 32 1618 32 101 32 78
Cuts at: 3433 3465 4935 4967 5673 5705 6698 6730 8113
Size: 32 1470 32 706 32 993 32 1383
Cuts at: 8113 8145 8187 8219 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1618 1470 1383 993 706 135 101
78 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6683 6816 7062 669
Size: 988 281 4745 133 246 3079
Fragments arranged by size:
4745 3079 988 281 246 133
HindIII A'AGCT_T
Cuts at: 748 3363 748
Size: 2615 6857
HpaI GTT'AAC
Cuts at: 7062 7062
Size: 9472
KpnI G_GTAC'C
Cuts at: 6797 6797
Size: 9472
MfeI C'AATT_G
Cuts at: 6435 6613 7071 6435
Size: 178 458 8836
Fragments arranged by size:
8836 458 178
MluI A'CGCG_T
Cuts at: 9348 9348
Size: 9472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2514 2860 5010 6223 8586 8770 1305
Size: 1209 346 2150 1213 2363 184 2007
Fragments arranged by size:
2363 2150 2007 1213 1209 346 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6176 6325 10
Size: 54 1425 343 4344 149 3157
Fragments arranged by size:
4344 3157 1425 343 149 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3798 5124 5136 5184 7430 7789 7948
Size: 648 2632 1326 12 48 2246 359 159
Cuts at: 7948 518
Size: 2042
Fragments arranged by size:
2632 2246 2042 1326 648 359 159 48
12
MspA1I CmG'CkG
Cuts at: 1606 3474 6888 7565 8713 8958 1606
Size: 1868 3414 677 1148 245 2120
Fragments arranged by size:
3414 2120 1868 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2734 513
Size: 552 939 730 7251
Fragments arranged by size:
7251 939 730 552
NdeI CA'TA_TG
Cuts at: 387 4783 6414 387
Size: 4396 1631 3445
Fragments arranged by size:
4396 3445 1631
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9472
NotI GC'GGCC_GC
Cuts at: 6919 6919
Size: 9472
NsiI A_TGCA'T
Cuts at: 5251 6420 5251
Size: 1169 8303
NspI r_CATG'y
Cuts at: 1132 1229 2487 5480 6830 8375 1132
Size: 97 1258 2993 1350 1545 2229
Fragments arranged by size:
2993 2229 1545 1350 1258 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 9472
PciI A'CATG_T
Cuts at: 2483 8371 2483
Size: 5888 3584
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3908 4226 5266 1108
Size: 2800 318 1040 5314
Fragments arranged by size:
5314 2800 1040 318
PmeI GTTT'AAAC
Cuts at: 6776 6776
Size: 9472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5036 5068 7541 7573 9054 9086 5036
Size: 32 2473 32 1481 32 5422
Fragments arranged by size:
5422 2473 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 9472
PsiI TTA'TAA
Cuts at: 7042 7042
Size: 9472
PstI C_TGCA'G
Cuts at: 830 4437 6824 830
Size: 3607 2387 3478
Fragments arranged by size:
3607 3478 2387
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9472
PvuII CAG'CTG
Cuts at: 3474 3474
Size: 9472
SacI G_AGCT'C
Cuts at: 2430 2430
Size: 9472
SalI G'TCGA_C
Cuts at: 1936 6814 1936
Size: 4878 4594
SapI GCTCTTCn'nnn_
Cuts at: 4776 4776
Size: 9472
SbfI CC_TGCA'GG
Cuts at: 6824 6824
Size: 9472
ScaI AGT'ACT
Cuts at: 2896 4439 7720 9432 2896
Size: 1543 3281 1712 2936
Fragments arranged by size:
3281 2936 1712 1543
SfcI C'TryA_G
Cuts at: 826 1045 2546 4433 4455 6820 7955 8636 8827
Size: 219 1501 1887 22 2365 1135 681 191
Cuts at: 8827 826
Size: 1471
Fragments arranged by size:
2365 1887 1501 1471 1135 681 219 191
22
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9472
SmaI CCC'GGG
Cuts at: 1872 6799 1872
Size: 4927 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5482 6375 7576 8477 8739 820
Size: 197 926 3539 893 1201 901 262 1553
Fragments arranged by size:
3539 1553 1201 926 901 893 262 197
SnaBI TAC'GTA
Cuts at: 493 4378 7263 493
Size: 3885 2885 2702
Fragments arranged by size:
3885 2885 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9472
SphI G_CATG'C
Cuts at: 1132 6830 1132
Size: 5698 3774
SspI AAT'ATT
Cuts at: 5 52 4732 5854 7396 5
Size: 47 4680 1122 1542 2081
Fragments arranged by size:
4680 2081 1542 1122 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9472
StyI C'CwwG_G
Cuts at: 513 1065 2004 2734 2772 4239 4740 6911 513
Size: 552 939 730 38 1467 501 2171 3074
Fragments arranged by size:
3074 2171 1467 939 730 552 501 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3282 3756 7499 7516 7669 7854 8299
Size: 1255 1702 474 3743 17 153 185 445
Cuts at: 8299 325
Size: 1498
Fragments arranged by size:
3743 1702 1498 1255 474 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2390 2894 3240
Size: 274 80 33 51 1443 413 504 346
Cuts at: 3240 4384 4437 4467 7718 9430 96
Size: 1144 53 30 3251 1712 138
Fragments arranged by size:
3251 1712 1443 1144 504 413 346 274
138 80 53 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4924 4956 9432 9464 4924
Size: 32 4476 32 4932
Fragments arranged by size:
4932 4476 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9472
XbaI T'CTAG_A
Cuts at: 6808 6808
Size: 9472
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9472
XmaI C'CCGG_G
Cuts at: 1870 6797 1870
Size: 4927 4545
XmnI GAAnn'nnTTC
Cuts at: 4274 4733 4850 5051 7601 4274
Size: 459 117 201 2550 6145
Fragments arranged by size:
6145 2550 459 201 117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7280 276
Size: 53 83 186 936 5746 2468
Fragments arranged by size:
5746 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
Bpu10I BsiWI BstZ17I Bsu36I EcoO109I FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SfoI SrfI SwaI XcmI