Restriction map for
pFN21AA1997
(Circular) (Six-base) MAPSORT of: pFN21AA1997.seq Check: 2791 from: 1 to: 17626
pFN21AA1997 17626 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3193 3193
Size: 17626
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 15436 278
Size: 53 83 186 936 13900 2468
Fragments arranged by size:
13900 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6335 6956 9039 14623 14969 1937
Size: 4398 621 2083 5584 346 4594
Fragments arranged by size:
5584 4594 4398 2083 621 346
Acc65I G'GTAC_C
Cuts at: 14947 14947
Size: 17626
AclI AA'CG_TT
Cuts at: 1563 13138 15753 16126 1563
Size: 11575 2615 373 3063
Fragments arranged by size:
11575 3063 2615 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2274 2390 4995 5120 6386 6669 6888 9386
Size: 642 116 2605 125 1266 283 219 2498
Cuts at: 9386 10085 10182 10892 11384 11394 11445 11505 13502
Size: 699 97 710 492 10 51 60 1997
Cuts at: 13502 13582 13808 13839 13856 15689 17073 17341 1632
Size: 80 226 31 17 1833 1384 268 1917
Fragments arranged by size:
2605 2498 1997 1917 1833 1384 1266 710
699 642 492 283 268 226 219 125
116 97 80 60 51 31 17 10
AfeI AGC'GCT
Cuts at: 2927 4154 2927
Size: 1227 16399
AflII C'TTAA_G
Cuts at: 820 1017 8732 10256 820
Size: 197 7715 1524 8190
Fragments arranged by size:
8190 7715 1524 197
AflIII A'CryG_T
Cuts at: 1203 6920 10090 10633 12546 12801 16525 17502 1203
Size: 5717 3170 543 1913 255 3724 977 1327
Fragments arranged by size:
5717 3724 3170 1913 1327 977 543 255
AgeI A'CCGG_T
Cuts at: 16457 16457
Size: 17626
AhdI GACnn_n'nnGTC
Cuts at: 9706 16355 9706
Size: 6649 10977
AleI CACnn'nnGTG
Cuts at: 1166 2101 1166
Size: 935 16691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2165 8915 14825 1184
Size: 981 6750 5910 3985
Fragments arranged by size:
6750 5910 3985 981
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2775 2807 12618 12650 2775
Size: 32 9811 32 7751
Fragments arranged by size:
9811 7751 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2098 8995 10805 11468 15369 16941 2098
Size: 6897 1810 663 3901 1572 2783
Fragments arranged by size:
6897 3901 2783 1810 1572 663
ApaLI G'TGCA_C
Cuts at: 8961 12408 15683 16839 8961
Size: 3447 3275 1156 9748
Fragments arranged by size:
9748 3447 3275 1156
ApoI r'AATT_y
Cuts at: 1493 2483 3231 3399 3830 3958 4315 4657 5054
Size: 990 748 168 431 128 357 342 397
Cuts at: 5054 5162 5391 5486 6025 6199 6254 6641 6849
Size: 108 229 95 539 174 55 387 208
Cuts at: 6849 6947 7229 7348 7362 8203 8564 8624 9175
Size: 98 282 119 14 841 361 60 551
Cuts at: 9175 9399 9452 9874 10317 10360 10537 11010 11319
Size: 224 53 422 443 43 177 473 309
Cuts at: 11319 11444 11714 12098 12450 13519 13673 13751 13962
Size: 125 270 384 352 1069 154 78 211
Cuts at: 13962 14093 14161 14600 14935 15161 15313 17422 1493
Size: 131 68 439 335 226 152 2109 1697
Fragments arranged by size:
2109 1697 1069 990 841 748 551 539
473 443 439 431 422 397 387 384
361 357 352 342 335 309 282 270
229 226 224 211 208 177 174 168
154 152 131 128 125 119 108 98
95 78 68 60 55 53 43 14
AseI AT'TA_AT
Cuts at: 160 1032 4734 5837 8574 10574 11532 12540 16180
Size: 872 3702 1103 2737 2000 958 1008 3640
Cuts at: 16180 160
Size: 1606
Fragments arranged by size:
3702 3640 2737 2000 1606 1103 1008 958
872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 17626
AvaI C'yCGr_G
Cuts at: 1870 1943 3081 14951 1870
Size: 73 1138 11870 4545
Fragments arranged by size:
11870 4545 1138 73
AvrII C'CTAG_G
Cuts at: 5663 5663
Size: 17626
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4833 4866 5709 5742 9416 9449 4833
Size: 33 843 33 3674 33 13010
Fragments arranged by size:
13010 3674 843 33 33 33
BamHI G'GATC_C
Cuts at: 6751 9545 14956 15323 6751
Size: 2794 5411 367 9054
Fragments arranged by size:
9054 5411 2794 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3390 13730 14947 16402 618
Size: 325 217 627 1603 10340 1217 1455 1842
Fragments arranged by size:
10340 1842 1603 1455 1217 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3141 9689 9739 10242 11617 12595 13588 14945
Size: 1750 6548 50 503 1375 978 993 1357
Cuts at: 14945 1391
Size: 4072
Fragments arranged by size:
6548 4072 1750 1375 1357 993 978 503
50
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4757 4983 5126 5451 5856 6674 6876
Size: 971 2858 226 143 325 405 818 202
Cuts at: 6876 7132 7411 7619 8751 9241 9250 10188 10288
Size: 256 279 208 1132 490 9 938 100
Cuts at: 10288 11145 11382 928
Size: 857 237 7172
Fragments arranged by size:
7172 2858 1132 971 938 857 818 490
405 325 279 256 237 226 208 202
143 100 9
BbvCI CC'TCA_GC
Cuts at: 7578 7578
Size: 17626
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5366 5400 12713 12747 15817 15851 1903
Size: 34 3429 34 7313 34 3070 34 3678
Fragments arranged by size:
7313 3678 3429 3070 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2519 8680 12766 15518 16728 2519
Size: 6161 4086 2752 1210 3417
Fragments arranged by size:
6161 4086 3417 2752 1210
BclI T'GATC_A
Cuts at: 1548 2725 3886 6220 6913 10179 10818 12460 12703
Size: 1177 1161 2334 693 3266 639 1642 243
Cuts at: 12703 12838 14031 1548
Size: 135 1193 5143
Fragments arranged by size:
5143 3266 2334 1642 1193 1177 1161 693
639 243 135
BfrBI ATG'CAT
Cuts at: 3413 4231 4235 10018 12742 14774 3413
Size: 818 4 5783 2724 2032 6265
Fragments arranged by size:
6265 5783 2724 2032 818 4
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 16237 136
Size: 107 122 71 631 605 14565 1525
Fragments arranged by size:
14565 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3568 3653 4537 5139 8365 12031 13687 17621 3568
Size: 85 884 602 3226 3666 1656 3934 3573
Fragments arranged by size:
3934 3666 3573 3226 1656 884 602 85
BlpI GC'TnA_GC
Cuts at: 4189 6274 11241 15043 4189
Size: 2085 4967 3802 6772
Fragments arranged by size:
6772 4967 3802 2085
Bme1580I G_kGCm'C
Cuts at: 1790 5389 5767 8653 8965 11837 12412 13318 14449
Size: 3599 378 2886 312 2872 575 906 1131
Cuts at: 14449 15687 16843 1790
Size: 1238 1156 2573
Fragments arranged by size:
3599 2886 2872 2573 1238 1156 1131 906
575 378 312
BmgBI CAC'GTC
Cuts at: 1318 5132 1318
Size: 3814 13812
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4879 6576 6883
Size: 416 53 83 399 24 3462 1697 307
Cuts at: 6883 7092 8235 9248 12900 14473 15357 16315 442
Size: 209 1143 1013 3652 1573 884 958 1753
Fragments arranged by size:
3652 3462 1753 1697 1573 1143 1013 958
884 416 399 307 209 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 12305 1056
Size: 11249 6377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 13349 14821 13349
Size: 1472 16154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4295 5607 6800 8649 9249 9966 10779 13488
Size: 3056 1312 1193 1849 600 717 813 2709
Cuts at: 13488 16286 1239
Size: 2798 2579
Fragments arranged by size:
3056 2798 2709 2579 1849 1312 1193 813
717 600
Bpu10I CC'TnA_GC
Cuts at: 3934 7578 8906 13551 3934
Size: 3644 1328 4645 8009
Fragments arranged by size:
8009 4645 3644 1328
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6564 7017 8124 9809 10160 11498 11729 12027 12330
Size: 453 1107 1685 351 1338 231 298 303
Cuts at: 12330 15751 16616 16914 6564
Size: 3421 865 298 7276
Fragments arranged by size:
7276 3421 1685 1338 1107 865 453 351
303 298 298 231
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 16289 882
Size: 617 14790 2219
Fragments arranged by size:
14790 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5798 12836 15417 493
Size: 711 4594 7038 2581 2702
Fragments arranged by size:
7038 4594 2702 2581 711
BsaBI GATnn'nnATC
Cuts at: 2271 2730 4911 5316 8220 11078 11353 12477 14691
Size: 459 2181 405 2904 2858 275 1124 2214
Cuts at: 14691 17620 2271
Size: 2929 2277
Fragments arranged by size:
2929 2904 2858 2277 2214 2181 1124 459
405 275
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 15433 15815 16450 275
Size: 53 83 186 936 13900 382 635 1451
Fragments arranged by size:
13900 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 8928 12145 13051 14791 16059 16457 16731 16878 17289
Size: 3217 906 1740 1268 398 274 147 411
Cuts at: 17289 8928
Size: 9265
Fragments arranged by size:
9265 3217 1740 1268 906 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4268 4298 6438 6468 9899
Size: 30 165 30 2865 30 2140 30 3431
Cuts at: 9899 9929 10421 10451 1178
Size: 30 492 30 8353
Fragments arranged by size:
8353 3431 2865 2140 492 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 8652 11411 11740 14181 1186
Size: 958 6508 2759 329 2441 4631
Fragments arranged by size:
6508 4631 2759 2441 958 329
BseYI C'CCAG_C
Cuts at: 2432 12873 13697 14723 16829 17325 2432
Size: 10441 824 1026 2106 496 2733
Fragments arranged by size:
10441 2733 2106 1026 824 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2183 3759 6606 11247 1163
Size: 573 447 1576 2847 4641 7542
Fragments arranged by size:
7542 4641 2847 1576 573 447
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6076 14203 15076 15837 16865 1540
Size: 275 186 4075 8127 873 761 1028 2301
Fragments arranged by size:
8127 4075 2301 1028 873 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 8119 8965 9739 11617 12412 12595 13588 14742 15687
Size: 846 774 1878 795 183 993 1154 945
Cuts at: 15687 15772 16843 8119
Size: 85 1071 8902
Fragments arranged by size:
8902 1878 1154 1071 993 945 846 795
774 183 85
BsmI GAATG_Cn'
Cuts at: 3263 4428 6278 7681 9131 9495 11242 11569 15137
Size: 1165 1850 1403 1450 364 1747 327 3568
Cuts at: 15137 15230 3263
Size: 93 5659
Fragments arranged by size:
5659 3568 1850 1747 1450 1403 1165 364
327 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5654 12019 5654
Size: 6365 11261
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3141 5389 5767 8119 8653 8965 9689
Size: 399 1351 2248 378 2352 534 312 724
Cuts at: 9689 9739 10242 11617 11837 12412 12595 13318 13588
Size: 50 503 1375 220 575 183 723 270
Cuts at: 13588 14449 14742 14945 15687 15772 16843 1391
Size: 861 293 203 742 85 1071 2174
Fragments arranged by size:
2352 2248 2174 1375 1351 1071 861 742
724 723 575 534 503 399 378 312
293 270 220 203 183 85 50
BspEI T'CCGG_A
Cuts at: 14791 17289 14791
Size: 2498 15128
BspHI T'CATG_A
Cuts at: 3025 8293 8557 10371 15515 17245 3025
Size: 5268 264 1814 5144 1730 3406
Fragments arranged by size:
5268 5144 3406 1814 1730 264
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3058 3193 6027 7828 14981 844
Size: 2214 135 2834 1801 7153 3489
Fragments arranged by size:
7153 3489 2834 2214 1801 135
BsrBI CCG'CTC
Cuts at: 1394 1823 10025 15513 1394
Size: 429 8202 5488 3507
Fragments arranged by size:
8202 5488 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3819 6083 6435 13327 16121 16295 65
Size: 1183 2571 2264 352 6892 2794 174 1396
Fragments arranged by size:
6892 2794 2571 2264 1396 1183 352 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 16270 16457 1700
Size: 110 14460 187 2869
Fragments arranged by size:
14460 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4263 4639 10156 13741 13756 96
Size: 4167 376 5517 3585 15 3966
Fragments arranged by size:
5517 4167 3966 3585 376 15
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 17626
BssSI C'ACGA_G
Cuts at: 2502 8652 15686 16698 2502
Size: 6150 7034 1012 3430
Fragments arranged by size:
7034 6150 3430 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3821 10330 10601 10805 12407 794
Size: 3027 6509 271 204 1602 6013
Fragments arranged by size:
6509 6013 3027 1602 271 204
BstBI TT'CG_AA
Cuts at: 16430 16430
Size: 17626
BstEII G'GTnAC_C
Cuts at: 1184 14845 1184
Size: 13661 3965
BstXI CCAn_nnnn'nTGG
Cuts at: 5631 7045 9124 5631
Size: 1414 2079 14133
Fragments arranged by size:
14133 2079 1414
BstYI r'GATC_y
Cuts at: 1972 2827 3568 3653 4537 5139 6751 8365 9416
Size: 855 741 85 884 602 1612 1614 1051
Cuts at: 9416 9545 11848 12031 13687 14072 14210 14410 14956
Size: 129 2303 183 1656 385 138 200 546
Cuts at: 14956 15323 15708 15725 17177 17263 17275 17621 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2303 1977 1656 1614 1612 1452 1051 884
855 741 602 546 385 385 367 346
200 183 138 129 86 85 17 12
BstZ17I GTA'TAC
Cuts at: 6957 9040 6957
Size: 2083 15543
Bsu36I CC'TnA_GG
Cuts at: 2156 13650 2156
Size: 11494 6132
BtgI C'CryG_G
Cuts at: 513 1065 2004 2557 2942 6076 8834 12879 13071
Size: 552 939 553 385 3134 2758 4045 192
Cuts at: 13071 13266 513
Size: 195 4873
Fragments arranged by size:
4873 4045 3134 2758 939 553 552 385
195 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2538 487
Size: 683 56 1312 15575
Fragments arranged by size:
15575 1312 683 56
BtsI GCAGTG_nn'
Cuts at: 5832 9979 12412 13118 13431 14580 15142 15936 15956
Size: 4147 2433 706 313 1149 562 794 20
Cuts at: 15956 5832
Size: 7502
Fragments arranged by size:
7502 4147 2433 1149 794 706 562 313
20
ClaI AT'CG_AT
Cuts at: 1554 7161 1554
Size: 5607 12019
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3330 3365 8923 8958 14556 14591 14867
Size: 35 2766 35 5558 35 5598 35 276
Cuts at: 14867 14902 529
Size: 35 3253
Fragments arranged by size:
5598 5558 3253 2766 276 35 35 35
35 35
DraI TTT'AAA
Cuts at: 2599 4663 4728 5167 5754 6030 6463 7510 11209
Size: 2064 65 439 587 276 433 1047 3699
Cuts at: 11209 13512 14930 2599
Size: 2303 1418 5295
Fragments arranged by size:
5295 3699 2303 2064 1418 1047 587 439
433 276 65
DraIII CAC_nnn'GTG
Cuts at: 5771 5771
Size: 17626
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6164 16633 809
Size: 507 4848 10469 1802
Fragments arranged by size:
10469 4848 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5215 5436 6073
Size: 54 1425 50 275 18 3385 221 637
Cuts at: 6073 15073 15962 17591 8
Size: 9000 889 1629 43
Fragments arranged by size:
9000 3385 1629 1425 889 637 275 221
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6073 15073 1537
Size: 275 4261 9000 4090
Fragments arranged by size:
9000 4261 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 2994 3054 4409 5328 5403 7842 8352
Size: 689 952 60 1355 919 75 2439 510
Cuts at: 8352 8787 11607 12327 13404 13601 14496 14556 15556
Size: 435 2820 720 1077 197 895 60 1000
Cuts at: 15556 1353
Size: 3423
Fragments arranged by size:
3423 2820 2439 1355 1077 1000 952 919
895 720 689 510 435 197 75 60
60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6644 12092 16213 16587 16733 387
Size: 1064 138 5055 5448 4121 374 146 1280
Fragments arranged by size:
5448 5055 4121 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 9737 11615 12593 13586 9737
Size: 1878 978 993 13777
Fragments arranged by size:
13777 1878 993 978
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2274 2390 4295 4995 5120 5607 6386
Size: 393 642 116 1905 700 125 487 779
Cuts at: 6386 6669 6800 6888 8649 9249 9386 9966 10085
Size: 283 131 88 1761 600 137 580 119
Cuts at: 10085 10182 10779 10892 11384 11394 11445 11505 13488
Size: 97 597 113 492 10 51 60 1983
Cuts at: 13488 13502 13582 13808 13839 13856 15689 16286 17073
Size: 14 80 226 31 17 1833 597 787
Cuts at: 17073 17341 1239
Size: 268 1524
Fragments arranged by size:
1983 1905 1833 1761 1524 787 779 700
642 600 597 597 580 492 487 393
283 268 226 137 131 125 119 116
113 97 88 80 60 51 31 17
14 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2999 5480 9140 2999
Size: 2481 3660 11485
Fragments arranged by size:
11485 3660 2481
EcoO109I rG'GnC_Cy
Cuts at: 4371 7209 4371
Size: 2838 14788
EcoRI G'AATT_C
Cuts at: 3399 3958 8203 8564 10317 11319 12098 14161 14935
Size: 559 4245 361 1753 1002 779 2063 774
Cuts at: 14935 15313 3399
Size: 378 5712
Fragments arranged by size:
5712 4245 2063 1753 1002 779 774 559
378 361
EcoRV GAT'ATC
Cuts at: 5374 6148 8051 9085 5374
Size: 774 1903 1034 13915
Fragments arranged by size:
13915 1903 1034 774
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3348 7036 7858 11219 12338 12443 12529 3348
Size: 3688 822 3361 1119 105 86 8445
Fragments arranged by size:
8445 3688 3361 1119 822 105 86
FspI TGC'GCA
Cuts at: 16132 16132
Size: 17626
HaeII r_GCGC'y
Cuts at: 1678 2929 4156 9604 16773 1678
Size: 1251 1227 5448 7169 2531
Fragments arranged by size:
7169 5448 2531 1251 1227
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2665 3602 3617 3634
Size: 32 135 32 1061 32 937 15 17
Cuts at: 3634 3638 3649 3670 3889 3921 4750 4782 5059
Size: 4 11 21 219 32 829 32 277
Cuts at: 5059 5091 5131 5163 5913 5945 5963 5995 6116
Size: 32 40 32 750 32 18 32 121
Cuts at: 6116 6148 6343 6375 6395 6427 7154 7186 8135
Size: 32 195 32 20 32 727 32 949
Cuts at: 8135 8167 8182 8214 9671 9692 9703 9724 9899
Size: 32 15 32 1457 21 11 21 175
Cuts at: 9899 9931 10421 10453 12016 12048 12974 13006 14023
Size: 32 490 32 1563 32 926 32 1017
Cuts at: 14023 14055 16267 16299 16341 16373 1373
Size: 32 2212 32 42 32 2626
Fragments arranged by size:
2626 2212 1563 1457 1061 1017 949 937
926 829 750 727 490 277 219 195
175 135 121 42 40 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 21 21 21 20 18
17 15 15 11 11 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2668 3454 5566 6592 8275 12117
Size: 988 281 730 786 2112 1026 1683 3842
Cuts at: 12117 12175 14624 14970 15216 669
Size: 58 2449 346 246 3079
Fragments arranged by size:
3842 3079 2449 2112 1683 1026 988 786
730 346 281 246 58
HindIII A'AGCT_T
Cuts at: 748 5790 6371 10266 748
Size: 5042 581 3895 8108
Fragments arranged by size:
8108 5042 3895 581
HpaI GTT'AAC
Cuts at: 8275 12175 15216 8275
Size: 3900 3041 10685
Fragments arranged by size:
10685 3900 3041
KpnI G_GTAC'C
Cuts at: 14951 14951
Size: 17626
MfeI C'AATT_G
Cuts at: 2616 5721 15225 2616
Size: 3105 9504 5017
Fragments arranged by size:
9504 5017 3105
MluI A'CGCG_T
Cuts at: 17502 17502
Size: 17626
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2196 2217 3320 3459 5800 5890 9968 10960
Size: 891 21 1103 139 2341 90 4078 992
Cuts at: 10960 12601 12610 12775 13637 16740 16924 1305
Size: 1641 9 165 862 3103 184 2007
Fragments arranged by size:
4078 3103 2341 2007 1641 1103 992 891
862 184 165 139 90 21 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5217 5438 10
Size: 54 1425 343 3385 221 12198
Fragments arranged by size:
12198 3385 1425 343 221 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2101 3558 4830 7043 7163 8541 8958
Size: 648 935 1457 1272 2213 120 1378 417
Cuts at: 8958 10017 15584 15943 16102 518
Size: 1059 5567 359 159 2042
Fragments arranged by size:
5567 2213 2042 1457 1378 1272 1059 935
648 417 359 159 120
MspA1I CmG'CkG
Cuts at: 1606 4559 7076 9227 11261 11702 14084 15042 15719
Size: 2953 2517 2151 2034 441 2382 958 677
Cuts at: 15719 16867 17112 1606
Size: 1148 245 2120
Fragments arranged by size:
2953 2517 2382 2151 2120 2034 1148 958
677 441 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2942 8834 13071 13266 513
Size: 552 939 938 5892 4237 195 4873
Fragments arranged by size:
5892 4873 4237 939 938 552 195
NdeI CA'TA_TG
Cuts at: 387 3071 8725 9589 10597 12663 13492 387
Size: 2684 5654 864 1008 2066 829 4521
Fragments arranged by size:
5654 4521 2684 2066 1008 864 829
NheI G'CTAG_C
Cuts at: 1052 12301 1052
Size: 11249 6377
NotI GC'GGCC_GC
Cuts at: 15073 15073
Size: 17626
NsiI A_TGCA'T
Cuts at: 3415 4233 4237 10020 12744 14776 3415
Size: 818 4 5783 2724 2032 6265
Fragments arranged by size:
6265 5783 2724 2032 818 4
NspI r_CATG'y
Cuts at: 1132 1229 4231 4235 6924 8809 10018 10094 10637
Size: 97 3002 4 2689 1885 1209 76 543
Cuts at: 10637 11029 12550 12742 12805 13089 13626 14778 14984
Size: 392 1521 192 63 284 537 1152 206
Cuts at: 14984 16529 1132
Size: 1545 2229
Fragments arranged by size:
3002 2689 2229 1885 1545 1521 1209 1152
543 537 392 284 206 192 97 76
63 4
PacI TTA_AT'TAA
Cuts at: 11532 11532
Size: 17626
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 17626
PciI A'CATG_T
Cuts at: 6920 10090 10633 12546 12801 16525 6920
Size: 3170 543 1913 255 3724 8021
Fragments arranged by size:
8021 3724 3170 1913 543 255
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3428 4891 7921 11311 1108
Size: 2320 1463 3030 3390 7423
Fragments arranged by size:
7423 3390 3030 2320 1463
PfoI T'CCnGG_A
Cuts at: 6777 7783 10210 6777
Size: 1006 2427 14193
Fragments arranged by size:
14193 2427 1006
PmeI GTTT'AAAC
Cuts at: 14930 14930
Size: 17626
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 15695 15727 17208 17240 15695
Size: 32 1481 32 16081
Fragments arranged by size:
16081 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 17626
PsiI TTA'TAA
Cuts at: 2634 3876 5958 12960 15196 2634
Size: 1242 2082 7002 2236 5064
Fragments arranged by size:
7002 5064 2236 2082 1242
PspXI vC'TCGA_Gb
Cuts at: 3081 3081
Size: 17626
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 9336 9368 11991 12023 9336
Size: 32 2623 32 14939
Fragments arranged by size:
14939 2623 32 32
PstI C_TGCA'G
Cuts at: 830 6024 8991 11260 11521 13438 13931 14978 830
Size: 5194 2967 2269 261 1917 493 1047 3478
Fragments arranged by size:
5194 3478 2967 2269 1917 1047 493 261
PvuI CG_AT'CG
Cuts at: 2001 14203 2001
Size: 12202 5424
PvuII CAG'CTG
Cuts at: 4559 7076 9227 11261 11702 14084 4559
Size: 2517 2151 2034 441 2382 8101
Fragments arranged by size:
8101 2517 2382 2151 2034 441
SacI G_AGCT'C
Cuts at: 9739 11617 12595 13588 9739
Size: 1878 978 993 13777
Fragments arranged by size:
13777 1878 993 978
SalI G'TCGA_C
Cuts at: 1936 14622 14968 1936
Size: 12686 346 4594
Fragments arranged by size:
12686 4594 346
SapI GCTCTTCn'nnn_
Cuts at: 2042 5328 14496 14556 2042
Size: 3286 9168 60 5112
Fragments arranged by size:
9168 5112 3286 60
SbfI CC_TGCA'GG
Cuts at: 14978 14978
Size: 17626
ScaI AGT'ACT
Cuts at: 9022 9121 11254 15874 17586 9022
Size: 99 2133 4620 1712 9062
Fragments arranged by size:
9062 4620 2133 1712 99
SexAI A'CCwGG_T
Cuts at: 13257 13257
Size: 17626
SfcI C'TryA_G
Cuts at: 826 1045 4197 6020 8946 8987 9291 11256 11517
Size: 219 3152 1823 2926 41 304 1965 261
Cuts at: 11517 11865 13434 13469 13927 14974 16109 16790 16981
Size: 348 1569 35 458 1047 1135 681 191
Cuts at: 16981 826
Size: 1471
Fragments arranged by size:
3152 2926 1965 1823 1569 1471 1135 1047
681 458 348 304 261 219 191 41
35
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 17626
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 17626
SmaI CCC'GGG
Cuts at: 1872 14953 1872
Size: 13081 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3081 6579 7032 8139 8732 9824
Size: 197 926 1138 3498 453 1107 593 1092
Cuts at: 9824 10139 10256 11477 11708 12042 12345 15730 16631
Size: 315 117 1221 231 334 303 3385 901
Cuts at: 16631 16893 820
Size: 262 1553
Fragments arranged by size:
3498 3385 1553 1221 1138 1107 1092 926
901 593 453 334 315 303 262 231
197 117
SnaBI TAC'GTA
Cuts at: 493 15417 493
Size: 14924 2702
SpeI A'CTAG_T
Cuts at: 152 5672 152
Size: 5520 12106
SphI G_CATG'C
Cuts at: 1132 4235 11029 12742 14984 1132
Size: 3103 6794 1713 2242 3774
Fragments arranged by size:
6794 3774 3103 2242 1713
SspI AAT'ATT
Cuts at: 5 52 2960 4129 4400 4732 6324 6823 7185
Size: 47 2908 1169 271 332 1592 499 362
Cuts at: 7185 9202 11167 11293 11817 12355 14854 15550 5
Size: 2017 1965 126 524 538 2499 696 2081
Fragments arranged by size:
2908 2499 2081 2017 1965 1592 1169 696
538 524 499 362 332 271 126 47
StuI AGG'CCT
Cuts at: 1517 11775 1517
Size: 10258 7368
StyI C'CwwG_G
Cuts at: 513 1065 2004 2942 5653 5663 8834 13071 13266
Size: 552 939 938 2711 10 3171 4237 195
Cuts at: 13266 15065 513
Size: 1799 3074
Fragments arranged by size:
4237 3171 3074 2711 1799 939 938 552
195 10
SwaI ATTT'AAAT
Cuts at: 5754 6030 5754
Size: 276 17350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2875 4442 6040 11086 11269 15653 15670
Size: 1255 1295 1567 1598 5046 183 4384 17
Cuts at: 15670 15823 16008 16453 325
Size: 153 185 445 1498
Fragments arranged by size:
5046 4384 1598 1567 1498 1295 1255 445
185 183 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4263 4639 7252
Size: 274 80 33 51 1443 2286 376 2613
Cuts at: 7252 9020 9072 9119 10156 10945 11252 11738 12510
Size: 1768 52 47 1037 789 307 486 772
Cuts at: 12510 13036 13741 13756 14263 14335 15872 17584 96
Size: 526 705 15 507 72 1537 1712 138
Fragments arranged by size:
2613 2286 1768 1712 1537 1443 1037 789
772 705 526 507 486 376 307 274
138 80 72 52 51 47 33 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2493 2525 4197 4229 7363 7395 8149 8181 14881
Size: 32 1672 32 3134 32 754 32 6700
Cuts at: 14881 14913 17586 17618 2493
Size: 32 2673 32 2501
Fragments arranged by size:
6700 3134 2673 2501 1672 754 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 10686 11695 1316
Size: 9370 1009 7247
Fragments arranged by size:
9370 7247 1009
XbaI T'CTAG_A
Cuts at: 5677 8327 8818 9193 12325 14962 5677
Size: 2650 491 375 3132 2637 8341
Fragments arranged by size:
8341 3132 2650 2637 491 375
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5189 9512 5189
Size: 4323 13303
XhoI C'TCGA_G
Cuts at: 1943 3081 1943
Size: 1138 16488
XmaI C'CCGG_G
Cuts at: 1870 14951 1870
Size: 13081 4545
XmnI GAAnn'nnTTC
Cuts at: 2036 2063 4418 4698 5412 7488 8253 9201 9348
Size: 27 2355 280 714 2076 765 948 147
Cuts at: 9348 10070 11111 11167 11199 11319 11445 12006 12321
Size: 722 1041 56 32 120 126 561 315
Cuts at: 12321 12753 15755 2036
Size: 432 3002 3907
Fragments arranged by size:
3907 3002 2355 2076 1041 948 765 722
714 561 432 315 280 147 126 120
56 32 27
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 15434 276
Size: 53 83 186 936 13900 2468
Fragments arranged by size:
13900 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI BbeI BsiWI FseI FspAI KasI NaeI
NarI NgoMIV NruI PmlI PpuMI PspOMI RsrII SacII
SanDI SfiI SfoI SrfI