Restriction map for
pFN21AA1996
(Circular) (Six-base) MAPSORT of: pFN21AA1996.seq Check: 851 from: 1 to: 5953
pFN21AA1996 5953 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2740 2914 2740
Size: 174 5779
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3296 1937
Size: 1359 4594
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 3176 3252 4080 4453 1563
Size: 1613 76 828 373 3063
Fragments arranged by size:
3063 1613 828 373 76
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2460 3026 4016 5400 5668 1632
Size: 828 566 990 1384 268 1917
Fragments arranged by size:
1917 1384 990 828 566 268
AfeI AGC'GCT
Cuts at: 2951 2951
Size: 5953
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5756
AflIII A'CryG_T
Cuts at: 1203 4852 5829 1203
Size: 3649 977 1327
Fragments arranged by size:
3649 1327 977
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 2968 4682 2968
Size: 1714 4239
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2772 1184
Size: 1588 4365
AlwNI CAG_nnn'CTG
Cuts at: 3049 3058 3696 5268 3049
Size: 9 638 1572 3734
Fragments arranged by size:
3734 1572 638 9
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 2338 2541 2689 2855 3262 3488 3640 5749
Size: 845 203 148 166 407 226 152 2109
Cuts at: 5749 1493
Size: 1697
Fragments arranged by size:
2109 1697 845 407 226 203 166 152
148
AseI AT'TA_AT
Cuts at: 160 1032 4507 160
Size: 872 3475 1606
Fragments arranged by size:
3475 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 2973 3278 1870
Size: 73 1030 305 4545
Fragments arranged by size:
4545 1030 305 73
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3274 4729 618
Size: 325 217 627 1487 1455 1842
Fragments arranged by size:
1842 1487 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2960 3272 1391
Size: 1569 312 4072
Fragments arranged by size:
4072 1569 312
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4982
BbvCI CC'TCA_GC
Cuts at: 3117 3117
Size: 5953
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4144 4178 1903
Size: 34 2207 34 3678
Fragments arranged by size:
3678 2207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 2518 1548
Size: 970 4983
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2167 5948 2167
Size: 3781 2172
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 4014 5170 1790
Size: 2224 1156 2573
Fragments arranged by size:
2573 2224 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5953
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 4642 442
Size: 416 53 83 399 24 2267 958 1753
Fragments arranged by size:
2267 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2999 2999
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4613 1239
Size: 3374 2579
Bpu10I CC'TnA_GC
Cuts at: 3117 3117
Size: 5953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2920 4078 4943 5241 2920
Size: 1158 865 298 3632
Fragments arranged by size:
3632 1158 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3127 4616 882
Size: 617 1628 1489 2219
Fragments arranged by size:
2219 1628 1489 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3760 4142 4777 275
Size: 53 83 186 936 2227 382 635 1451
Fragments arranged by size:
2227 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2176 2206 1178
Size: 30 165 30 773 30 4925
Fragments arranged by size:
4925 773 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2805 3105 1186
Size: 1619 300 4034
Fragments arranged by size:
4034 1619 300
BseYI C'CCAG_C
Cuts at: 2952 5156 5652 2952
Size: 2204 496 3253
Fragments arranged by size:
3253 2204 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5380
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3403 4164 5192 1540
Size: 275 186 1402 761 1028 2301
Fragments arranged by size:
2301 1402 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4014 4099 5170 4014
Size: 85 1071 4797
Fragments arranged by size:
4797 1071 85
BsmI GAATG_Cn'
Cuts at: 2496 3464 3557 2496
Size: 968 93 4892
Fragments arranged by size:
4892 968 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2960 3272 4014 4099 5170 1391
Size: 399 1170 312 742 85 1071 2174
Fragments arranged by size:
2174 1170 1071 742 399 312 85
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2740 2914 3308 844
Size: 1896 174 394 3489
Fragments arranged by size:
3489 1896 394 174
BsrBI CCG'CTC
Cuts at: 1394 1823 3840 1394
Size: 429 2017 3507
Fragments arranged by size:
3507 2017 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3101 4448 4622 65
Size: 1183 1853 1347 174 1396
Fragments arranged by size:
1853 1396 1347 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2919 4597 4784 1700
Size: 110 1109 1678 187 2869
Fragments arranged by size:
2869 1678 1109 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 3002 4013 5025 3002
Size: 1011 1012 3930
Fragments arranged by size:
3930 1012 1011
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5953
BstBI TT'CG_AA
Cuts at: 4757 4757
Size: 5953
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5953
BstXI CCAn_nnnn'nTGG
Cuts at: 3144 3144
Size: 5953
BstYI r'GATC_y
Cuts at: 1972 2167 3283 3650 4035 4052 5504 5590 5602
Size: 195 1116 367 385 17 1452 86 12
Cuts at: 5602 5948 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 195 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2682 513
Size: 552 1617 3784
Fragments arranged by size:
3784 1617 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 2236 3469 4263 4283 2236
Size: 1233 794 20 3906
Fragments arranged by size:
3906 1233 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2122 2157 529
Size: 35 1558 35 4325
Fragments arranged by size:
4325 1558 35 35
DraI TTT'AAA
Cuts at: 3257 3257
Size: 5953
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3400 4289 5918
Size: 54 1425 50 275 18 1570 889 1629
Cuts at: 5918 8
Size: 43
Fragments arranged by size:
1629 1570 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2253 2604 2866 2872 3883 1353
Size: 900 351 262 6 1011 3423
Fragments arranged by size:
3423 1011 900 351 262 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2856 4540 4914 5060 387
Size: 1064 138 1267 1684 374 146 1280
Fragments arranged by size:
1684 1280 1267 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2460 3026 4016 4613 5400 5668 1239
Size: 393 828 566 990 597 787 268 1524
Fragments arranged by size:
1524 990 828 787 597 566 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2970 2970
Size: 5953
EcoRI G'AATT_C
Cuts at: 2541 2855 3262 3640 2541
Size: 314 407 378 4854
Fragments arranged by size:
4854 407 378 314
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 2822 2953 5100 1678
Size: 1144 131 2147 2531
Fragments arranged by size:
2531 2147 1144 131
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2147 2179 2843 2875 2954
Size: 32 135 32 575 32 664 32 79
Cuts at: 2954 2986 3100 3132 4594 4626 4668 4700 1373
Size: 32 114 32 1462 32 42 32 2626
Fragments arranged by size:
2626 1462 664 575 135 114 79 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2678 3297 3543 669
Size: 988 281 740 619 246 3079
Fragments arranged by size:
3079 988 740 619 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5953
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2658 2985 5067 5251 1305
Size: 1353 327 2082 184 2007
Fragments arranged by size:
2082 2007 1353 327 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4131
Fragments arranged by size:
4131 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3142 3911 4270 4429 518
Size: 648 1976 769 359 159 2042
Fragments arranged by size:
2042 1976 769 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2814 3369 4046 5194 5439 1606
Size: 1208 555 677 1148 245 2120
Fragments arranged by size:
2120 1208 1148 677 555 245
NaeI GCC'GGC
Cuts at: 2921 2921
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 2682 513
Size: 552 1617 3784
Fragments arranged by size:
3784 1617 552
NdeI CA'TA_TG
Cuts at: 387 2032 3188 387
Size: 1645 1156 3152
Fragments arranged by size:
3152 1645 1156
NgoMIV G'CCGG_C
Cuts at: 2919 2919
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 3031 3311 4856 1132
Size: 97 1802 280 1545 2229
Fragments arranged by size:
2229 1802 1545 280 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 4852 4852
Size: 5953
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3189 1108
Size: 2081 3872
PfoI T'CCnGG_A
Cuts at: 2722 2722
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4022 4054 5535 5567 4022
Size: 32 1481 32 4408
Fragments arranged by size:
4408 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2970 2970
Size: 5953
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5953
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PstI C_TGCA'G
Cuts at: 830 3021 3054 3305 830
Size: 2191 33 251 3478
Fragments arranged by size:
3478 2191 251 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5953
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SanDI GG'GwC_CC
Cuts at: 2970 2970
Size: 5953
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 4201 5913 4201
Size: 1712 4241
SfcI C'TryA_G
Cuts at: 826 1045 3017 3050 3301 4436 5117 5308 826
Size: 219 1972 33 251 1135 681 191 1471
Fragments arranged by size:
1972 1471 1135 681 251 219 191 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5953
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2899 4057 4958 5220 820
Size: 197 926 956 1158 901 262 1553
Fragments arranged by size:
1553 1158 956 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 3877 5
Size: 47 3825 2081
Fragments arranged by size:
3825 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2682 2913 3392 513
Size: 552 1617 231 479 3074
Fragments arranged by size:
3074 1617 552 479 231
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2651 3980 3997 4150 4335 4780 325
Size: 1255 1071 1329 17 153 185 445 1498
Fragments arranged by size:
1498 1329 1255 1071 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4199 5911 96
Size: 274 80 33 51 1443 2222 1712 138
Fragments arranged by size:
2222 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2176 2208 5913 5945 2176
Size: 32 3705 32 2184
Fragments arranged by size:
3705 2184 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 3289 3289
Size: 5953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2680 2680
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 2709 4082 2709
Size: 1373 4580
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI