Restriction map for
pFN21AA1983
(Circular) (Six-base) MAPSORT of: pFN21AA1983.seq Check: 748 from: 1 to: 5923
pFN21AA1983 5923 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3733 278
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2175 2204 2669 3078 3986 5370 5638 1632
Size: 543 29 465 409 908 1384 268 1917
Fragments arranged by size:
1917 1384 908 543 465 409 268 29
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 2097 2522 4822 5799 1203
Size: 894 425 2300 977 1327
Fragments arranged by size:
2300 1327 977 894 425
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2676 1184
Size: 1492 4431
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3056 3088 3056
Size: 32 5891
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaI G_GGCC'C
Cuts at: 2774 2915 2979 2774
Size: 141 64 5718
Fragments arranged by size:
5718 141 64
ApaLI G'TGCA_C
Cuts at: 2308 3980 5136 2308
Size: 1672 1156 3095
Fragments arranged by size:
3095 1672 1156
ApoI r'AATT_y
Cuts at: 1493 2240 3122 3232 3458 3610 5719 1493
Size: 747 882 110 226 152 2109 1697
Fragments arranged by size:
2109 1697 882 747 226 152 110
AseI AT'TA_AT
Cuts at: 160 1032 4477 160
Size: 872 3445 1606
Fragments arranged by size:
3445 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 3248 1870
Size: 73 1305 4545
Fragments arranged by size:
4545 1305 73
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2008 2932 3078 3244 4699
Size: 325 217 627 221 924 146 166 1455
Cuts at: 4699 618
Size: 1842
Fragments arranged by size:
1842 1455 924 627 325 221 217 166
146
BanII G_rGCy'C
Cuts at: 1391 2202 2774 2855 2915 2979 3158 3205 3242
Size: 811 572 81 60 64 179 47 37
Cuts at: 3242 1391
Size: 4072
Fragments arranged by size:
4072 811 572 179 81 64 60 47
37
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2181 2525 928
Size: 971 282 344 4326
Fragments arranged by size:
4326 971 344 282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2264 2298 4114 4148 1903
Size: 34 327 34 1816 34 3678
Fragments arranged by size:
3678 1816 327 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2345 3815 5025 2345
Size: 1470 1210 3243
Fragments arranged by size:
3243 1470 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5923
BfrBI ATG'CAT
Cuts at: 2259 2259
Size: 5923
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 2493 2778
Size: 107 122 71 631 605 380 441 285
Cuts at: 2778 2850 2910 4534 136
Size: 72 60 1624 1525
Fragments arranged by size:
1624 1525 631 605 441 380 285 122
107 72 71 60
BglII A'GATC_T
Cuts at: 5918 5918
Size: 5923
BlpI GC'TnA_GC
Cuts at: 3340 3340
Size: 5923
Bme1580I G_kGCm'C
Cuts at: 1790 2312 2446 2774 2915 2935 2979 3081 3984
Size: 522 134 328 141 20 44 102 903
Cuts at: 3984 5140 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 903 522 328 141 134 102
44 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5923
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3215 3654 4612
Size: 416 53 83 399 24 1798 439 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1798 1753 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2973 3180 4583 1239
Size: 1734 207 1403 2579
Fragments arranged by size:
2579 1734 1403 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3217 4048 4913 5211 3217
Size: 831 865 298 3929
Fragments arranged by size:
3929 865 831 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2991 4586 882
Size: 617 1492 1595 2219
Fragments arranged by size:
2219 1595 1492 617
BsaAI yAC'GTr
Cuts at: 493 1204 2100 3714 493
Size: 711 896 1614 2702
Fragments arranged by size:
2702 1614 896 711
BsaBI GATnn'nnATC
Cuts at: 3168 5917 3168
Size: 2749 3174
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3730 4112 4747 275
Size: 53 83 186 936 2197 382 635 1451
Fragments arranged by size:
2197 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2376 3081 4356 4754 5028 5175 5586 2376
Size: 705 1275 398 274 147 411 2713
Fragments arranged by size:
2713 1275 705 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2050 2080 1178
Size: 30 165 30 647 30 5021
Fragments arranged by size:
5021 647 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2046 2129 2979 3116 1186
Size: 860 83 850 137 3993
Fragments arranged by size:
3993 860 850 137 83
BseYI C'CCAG_C
Cuts at: 2050 3128 5126 5622 2050
Size: 1078 1998 496 2351
Fragments arranged by size:
2351 1998 1078 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5350
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2202 2312 3984 4069 5140 2202
Size: 110 1672 85 1071 2985
Fragments arranged by size:
2985 1672 1071 110 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2312 2446 2774 2855 2915 2935
Size: 399 412 110 134 328 81 60 20
Cuts at: 2935 2979 3081 3158 3205 3242 3984 4069 5140
Size: 44 102 77 47 37 742 85 1071
Cuts at: 5140 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 412 399 328 134 110
102 85 81 77 60 47 44 37
20
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 3812 5542 3812
Size: 1730 4193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3278 844
Size: 2434 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2434 3049 4418 4592 65
Size: 1183 1186 615 1369 174 1396
Fragments arranged by size:
1396 1369 1186 1183 615 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4567 4754 1700
Size: 110 2757 187 2869
Fragments arranged by size:
2869 2757 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2638 794
Size: 1844 4079
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstXI CCAn_nnnn'nTGG
Cuts at: 3151 3151
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 3253 3620 4005 4022 5474 5560 5572 5918
Size: 1281 367 385 17 1452 86 12 346
Cuts at: 5918 1972
Size: 1977
Fragments arranged by size:
1977 1452 1281 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3117 3117
Size: 5923
BtgI C'CryG_G
Cuts at: 513 1065 2004 2846 2865 3144 513
Size: 552 939 842 19 279 3292
Fragments arranged by size:
3292 939 842 552 279 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5184
Fragments arranged by size:
5184 683 56
BtsI GCAGTG_nn'
Cuts at: 2312 3439 4233 4253 2312
Size: 1127 794 20 3982
Fragments arranged by size:
3982 1127 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2323 2557 3370
Size: 54 1425 50 275 18 493 234 813
Cuts at: 3370 4259 5888 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 813 493 275 234 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2935 3097 3853 1353
Size: 1582 162 756 3423
Fragments arranged by size:
3423 1582 756 162
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2200 2200
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2175 2204 2669 2973 3078 3180 3986
Size: 393 543 29 465 304 105 102 806
Cuts at: 3986 4583 5370 5638 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 806 787 597 543 465 393 304
268 105 102 29
EcoO109I rG'GnC_Cy
Cuts at: 2744 2770 2771 2825 2861 2911 2912 2993 2744
Size: 26 1 54 36 50 1 81 5674
Fragments arranged by size:
5674 81 54 50 36 26 1 1
EcoRI G'AATT_C
Cuts at: 3232 3610 3232
Size: 378 5545
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 2972 5070 1678
Size: 1294 2098 2531
Fragments arranged by size:
2531 2098 1294
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2050 2082 2244 2276 3140
Size: 32 135 32 478 32 162 32 864
Cuts at: 3140 3172 4564 4596 4638 4670 1373
Size: 32 1392 32 42 32 2626
Fragments arranged by size:
2626 1392 864 478 162 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2559 10
Size: 54 1425 343 727 3374
Fragments arranged by size:
3374 1425 727 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3881 4240 4399 518
Size: 648 2715 359 159 2042
Fragments arranged by size:
2715 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2071 2478 2647 2684 3339 4016 5164
Size: 444 21 407 169 37 655 677 1148
Cuts at: 5164 5409 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 655 444 407 245 169
37 21
NcoI C'CATG_G
Cuts at: 513 1065 2004 2865 3144 513
Size: 552 939 861 279 3292
Fragments arranged by size:
3292 939 861 552 279
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NruI TCG'CGA
Cuts at: 2162 2162
Size: 5923
NsiI A_TGCA'T
Cuts at: 2261 2261
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2211 2526 2610 3281 4826 1132
Size: 97 982 315 84 671 1545 2229
Fragments arranged by size:
2229 1545 982 671 315 97 84
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 2522 4822 2522
Size: 2300 3623
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5923
PfoI T'CCnGG_A
Cuts at: 2347 2502 2740 2989 2347
Size: 155 238 249 5281
Fragments arranged by size:
5281 249 238 155
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PmlI CAC'GTG
Cuts at: 2100 2100
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2744 2861 2993 2744
Size: 117 132 5674
Fragments arranged by size:
5674 132 117
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5923
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PspOMI G'GGCC_C
Cuts at: 2770 2911 2975 2770
Size: 141 64 5718
Fragments arranged by size:
5718 141 64
PstI C_TGCA'G
Cuts at: 830 2646 3063 3275 830
Size: 1816 417 212 3478
Fragments arranged by size:
3478 1816 417 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2050 2647 2684 2050
Size: 597 37 5289
Fragments arranged by size:
5289 597 37
SacI G_AGCT'C
Cuts at: 2202 2202
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SanDI GG'GwC_CC
Cuts at: 2861 2861
Size: 5923
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2107 2212 2642 3059 3271 4406 5087
Size: 219 1062 105 430 417 212 1135 681
Cuts at: 5087 5278 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1062 681 430 417 219 212
191 105
SfiI GGCCn_nnn'nGGCC
Cuts at: 2910 2910
Size: 5923
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 3250 1872
Size: 1378 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3196 4027 4928 5190 820
Size: 197 926 1253 831 901 262 1553
Fragments arranged by size:
1553 1253 926 901 831 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 2865 3144 3362 513
Size: 552 939 459 402 279 218 3074
Fragments arranged by size:
3074 939 552 459 402 279 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3950 3967 4120 4305 4750 325
Size: 1255 2370 17 153 185 445 1498
Fragments arranged by size:
2370 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2336 4169 5881
Size: 274 80 33 51 1443 359 1833 1712
Cuts at: 5881 96
Size: 138
Fragments arranged by size:
1833 1712 1443 359 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2425 2457 2722 2754 5883 5915 2425
Size: 32 265 32 3129 32 2433
Fragments arranged by size:
3129 2433 265 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2047 2047
Size: 5923
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 3248 1870
Size: 1378 4545
XmnI GAAnn'nnTTC
Cuts at: 4052 4052
Size: 5923
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3731 276
Size: 53 83 186 936 2197 2468
Fragments arranged by size:
2468 2197 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NruI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV PacI PspXI
PsrI RsrII SacII SapI SexAI SfoI SrfI SwaI