Restriction map for
pFN21AA1964
(Circular) (Six-base) MAPSORT of: pFN21AA1964.seq Check: 7238 from: 1 to: 7072
pFN21AA1964 7072 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4311 4311
Size: 7072
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4882 278
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4415 1937
Size: 2478 4594
Acc65I G'GTAC_C
Cuts at: 4287 4393 4287
Size: 106 6966
AclI AA'CG_TT
Cuts at: 1563 5199 5572 1563
Size: 3636 373 3063
Fragments arranged by size:
3636 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2177 2777 3443 5135 6519 6787 1632
Size: 545 600 666 1692 1384 268 1917
Fragments arranged by size:
1917 1692 1384 666 600 545 268
AfeI AGC'GCT
Cuts at: 2489 4367 2489
Size: 1878 5194
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6875
AflIII A'CryG_T
Cuts at: 1203 2664 3000 3002 5971 6948 1203
Size: 1461 336 2 2969 977 1327
Fragments arranged by size:
2969 1461 1327 977 336 2
AgeI A'CCGG_T
Cuts at: 5903 5903
Size: 7072
AhdI GACnn_n'nnGTC
Cuts at: 5801 5801
Size: 7072
AleI CACnn'nnGTG
Cuts at: 1166 3003 3005 1166
Size: 1837 2 5233
Fragments arranged by size:
5233 1837 2
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3339 4016 1184
Size: 2155 677 4240
Fragments arranged by size:
4240 2155 677
AlwNI CAG_nnn'CTG
Cuts at: 3379 4003 4018 4162 4815 6387 3379
Size: 624 15 144 653 1572 4064
Fragments arranged by size:
4064 1572 653 624 144 15
ApaI G_GGCC'C
Cuts at: 3096 3096
Size: 7072
ApaLI G'TGCA_C
Cuts at: 5129 6285 5129
Size: 1156 5916
ApoI r'AATT_y
Cuts at: 1493 4381 4607 4759 6868 1493
Size: 2888 226 152 2109 1697
Fragments arranged by size:
2888 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5626 160
Size: 872 4594 1606
Fragments arranged by size:
4594 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2141 2794 3395 3817 3959 4397
Size: 73 99 99 653 601 422 142 438
Cuts at: 4397 1870
Size: 4545
Fragments arranged by size:
4545 653 601 438 422 142 99 99
73
BamHI G'GATC_C
Cuts at: 2562 4402 4769 2562
Size: 1840 367 4865
Fragments arranged by size:
4865 1840 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2086 2129 2396 2464 2861
Size: 325 217 627 299 43 267 68 397
Cuts at: 2861 4087 4287 4393 5848 618
Size: 1226 200 106 1455 1842
Fragments arranged by size:
1842 1455 1226 627 397 325 299 267
217 200 106 68 43
BanII G_rGCy'C
Cuts at: 1391 2052 2210 2907 3096 3183 3487 4183 4391
Size: 661 158 697 189 87 304 696 208
Cuts at: 4391 1391
Size: 4072
Fragments arranged by size:
4072 697 696 661 304 208 189 158
87
BbeI G_GCGC'C
Cuts at: 2090 2468 2865 2090
Size: 378 397 6297
Fragments arranged by size:
6297 397 378
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6101
BbvCI CC'TCA_GC
Cuts at: 2475 3677 2475
Size: 1202 5870
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5263 5297 1903
Size: 34 50 34 3242 34 3678
Fragments arranged by size:
3678 3242 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4964 6174 4964
Size: 1210 5862
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7072
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2870 5683 136
Size: 107 122 71 631 605 1198 2813 1525
Fragments arranged by size:
2813 1525 1198 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2778 3573 7067 2778
Size: 795 3494 2783
Fragments arranged by size:
3494 2783 795
BlpI GC'TnA_GC
Cuts at: 4489 4489
Size: 7072
Bme1580I G_kGCm'C
Cuts at: 1790 2821 3096 4092 4105 5133 6289 1790
Size: 1031 275 996 13 1028 1156 2573
Fragments arranged by size:
2573 1156 1031 1028 996 275 13
BmgBI CAC'GTC
Cuts at: 1318 2188 3232 1318
Size: 870 1044 5158
Fragments arranged by size:
5158 1044 870
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4803 5761 442
Size: 416 53 83 399 24 3386 958 1753
Fragments arranged by size:
3386 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2041 2792 2852 2861 3314 3332 5732 1239
Size: 802 751 60 9 453 18 2400 2579
Fragments arranged by size:
2579 2400 802 751 453 60 18 9
Bpu10I CC'TnA_GC
Cuts at: 2279 2475 2823 3677 2279
Size: 196 348 854 5674
Fragments arranged by size:
5674 854 348 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5197 6062 6360 5197
Size: 865 298 5909
Fragments arranged by size:
5909 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5735 882
Size: 617 4236 2219
Fragments arranged by size:
4236 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2238 3003 4123 4863 493
Size: 711 1034 765 1120 740 2702
Fragments arranged by size:
2702 1120 1034 765 740 711
BsaBI GATnn'nnATC
Cuts at: 7066 7066
Size: 7072
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2087 2465 2529 2862
Size: 53 83 186 936 554 378 64 333
Cuts at: 2862 3851 4224 4879 5261 5896 275
Size: 989 373 655 382 635 1451
Fragments arranged by size:
1451 989 936 655 635 554 382 378
373 333 186 83 64 53
BsaWI w'CCGG_w
Cuts at: 4183 4200 5505 5903 6177 6324 6735 4183
Size: 17 1305 398 274 147 411 4520
Fragments arranged by size:
4520 1305 411 398 274 147 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2240 2270 4214 4244 1178
Size: 30 165 30 837 30 1944 30 4006
Fragments arranged by size:
4006 1944 837 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 2669 2750 2789 2870 3413 3515 3518
Size: 874 609 81 39 81 543 102 3
Cuts at: 3518 3521 3524 3536 3548 1186
Size: 3 3 12 12 4710
Fragments arranged by size:
4710 874 609 543 102 81 81 39
12 12 3 3 3
BseYI C'CCAG_C
Cuts at: 2490 2880 3095 3159 3766 4149 6275 6771 2490
Size: 390 215 64 607 383 2126 496 2791
Fragments arranged by size:
2791 2126 607 496 390 383 215 64
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2357 2561 3328 3617 3993 1163
Size: 573 621 204 767 289 376 4242
Fragments arranged by size:
4242 767 621 573 376 289 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2017 2094 2713 2765 4522 5283
Size: 275 186 16 77 619 52 1757 761
Cuts at: 5283 6311 1540
Size: 1028 2301
Fragments arranged by size:
2301 1757 1028 761 619 275 186 77
52 16
BsiHKAI G_wGCw'C
Cuts at: 2907 4128 5133 5218 6289 2907
Size: 1221 1005 85 1071 3690
Fragments arranged by size:
3690 1221 1071 1005 85
BsmI GAATG_Cn'
Cuts at: 4036 4583 4676 4036
Size: 547 93 6432
Fragments arranged by size:
6432 547 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2210 2821 2907 3096 3183 3487
Size: 399 262 158 611 86 189 87 304
Cuts at: 3487 4092 4105 4128 4183 4391 5133 5218 6289
Size: 605 13 23 55 208 742 85 1071
Cuts at: 6289 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 611 605 399 304 262
208 189 158 87 86 85 55 23
13
BspEI T'CCGG_A
Cuts at: 4183 6735 4183
Size: 2552 4520
BspHI T'CATG_A
Cuts at: 2952 4961 6691 2952
Size: 2009 1730 3333
Fragments arranged by size:
3333 2009 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3931 4311 4427 844
Size: 3087 380 116 3489
Fragments arranged by size:
3489 3087 380 116
BsrBI CCG'CTC
Cuts at: 1394 1823 2229 2251 2548 4959 1394
Size: 429 406 22 297 2411 3507
Fragments arranged by size:
3507 2411 429 406 297 22
BsrDI GCAATG_nn'
Cuts at: 65 1248 5567 5741 65
Size: 1183 4319 174 1396
Fragments arranged by size:
4319 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2089 2259 5716 5903 1700
Size: 110 279 170 3457 187 2869
Fragments arranged by size:
3457 2869 279 187 170 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7072
BssHII G'CGCG_C
Cuts at: 1925 2191 2411 2517 2519 1925
Size: 266 220 106 2 6478
Fragments arranged by size:
6478 266 220 106 2
BssSI C'ACGA_G
Cuts at: 5132 6144 5132
Size: 1012 6060
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4100 4162 794
Size: 3306 62 3704
Fragments arranged by size:
3704 3306 62
BstBI TT'CG_AA
Cuts at: 5876 5876
Size: 7072
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7072
BstYI r'GATC_y
Cuts at: 1972 2223 2562 2778 3573 4077 4402 4769 5154
Size: 251 339 216 795 504 325 367 385
Cuts at: 5154 5171 6623 6709 6721 7067 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 795 504 385 367 346 339
325 251 216 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2469 3327 4081 513
Size: 552 1404 858 754 3504
Fragments arranged by size:
3504 1404 858 754 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2301 2410 487
Size: 683 56 1075 109 5149
Fragments arranged by size:
5149 1075 683 109 56
BtsI GCAGTG_nn'
Cuts at: 3301 3908 4259 4588 5382 5402 3301
Size: 607 351 329 794 20 4971
Fragments arranged by size:
4971 794 607 351 329 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7072
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7037
DraI TTT'AAA
Cuts at: 4376 4376
Size: 7072
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6079 809
Size: 507 4763 1802
Fragments arranged by size:
4763 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2014 2058 2762
Size: 54 1425 50 275 18 184 44 704
Cuts at: 2762 3196 3478 3564 4519 5408 7037 8
Size: 434 282 86 955 889 1629 43
Fragments arranged by size:
1629 1425 955 889 704 434 282 275
184 86 54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2014 2762 4519 1537
Size: 275 202 748 1757 4090
Fragments arranged by size:
4090 1757 748 275 202
EarI CTCTTCn'nnn_
Cuts at: 1353 2687 3055 3227 3525 4349 5002 1353
Size: 1334 368 172 298 824 653 3423
Fragments arranged by size:
3423 1334 824 653 368 298 172
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5659 6033 6179 387
Size: 1064 138 4070 374 146 1280
Fragments arranged by size:
4070 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2905 2905
Size: 7072
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2041 2177 2777 2792 2852 2861 3314
Size: 393 409 136 600 15 60 9 453
Cuts at: 3314 3332 3443 5135 5732 6519 6787 1239
Size: 18 111 1692 597 787 268 1524
Fragments arranged by size:
1692 1524 787 600 597 453 409 393
268 136 111 60 18 15 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2174 2684 3115 3440 2174
Size: 510 431 325 5806
Fragments arranged by size:
5806 510 431 325
EcoO109I rG'GnC_Cy
Cuts at: 2377 3092 3120 3433 3971 4320 2377
Size: 715 28 313 538 349 5129
Fragments arranged by size:
5129 715 538 349 313 28
EcoRI G'AATT_C
Cuts at: 4381 4759 4381
Size: 378 6694
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2629 3238 2629
Size: 609 6463
FspI TGC'GCA
Cuts at: 4070 5578 4070
Size: 1508 5564
FspAI rTGC'GCAy
Cuts at: 4070 4070
Size: 7072
HaeII r_GCGC'y
Cuts at: 1678 2090 2156 2389 2468 2491 2865 3380 3886
Size: 412 66 233 79 23 374 515 506
Cuts at: 3886 4369 6219 1678
Size: 483 1850 2531
Fragments arranged by size:
2531 1850 515 506 483 412 374 233
79 66 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2770 2802 3392 3424 4214
Size: 32 135 32 1198 32 590 32 790
Cuts at: 4214 4246 4307 4339 5713 5745 5787 5819 1373
Size: 32 61 32 1374 32 42 32 2626
Fragments arranged by size:
2626 1374 1198 790 590 135 61 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2647 4416 4662 669
Size: 988 281 709 1769 246 3079
Fragments arranged by size:
3079 1769 988 709 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7072
HpaI GTT'AAC
Cuts at: 4662 4662
Size: 7072
KasI G'GCGC_C
Cuts at: 2086 2464 2861 2086
Size: 378 397 6297
Fragments arranged by size:
6297 397 378
KpnI G_GTAC'C
Cuts at: 4291 4397 4291
Size: 106 6966
MfeI C'AATT_G
Cuts at: 4671 4671
Size: 7072
MluI A'CGCG_T
Cuts at: 6948 6948
Size: 7072
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2616 2727 3471 6186 6370 1305
Size: 1311 111 744 2715 184 2007
Fragments arranged by size:
2715 2007 1311 744 184 111
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3198 3566 10
Size: 54 1425 343 1366 368 3516
Fragments arranged by size:
3516 1425 1366 368 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3003 3005 3365 3998 5030 5389 5548
Size: 648 1837 2 360 633 1032 359 159
Cuts at: 5548 518
Size: 2042
Fragments arranged by size:
2042 1837 1032 648 633 360 359 159
2
MspA1I CmG'CkG
Cuts at: 1606 2019 2296 3141 3421 3461 3748 3766 3829
Size: 413 277 845 280 40 287 18 63
Cuts at: 3829 4018 4149 4174 4488 5165 6313 6558 1606
Size: 189 131 25 314 677 1148 245 2120
Fragments arranged by size:
2120 1148 845 677 413 314 287 280
277 245 189 131 63 40 25 18
NarI GG'CG_CC
Cuts at: 2087 2465 2862 2087
Size: 378 397 6297
Fragments arranged by size:
6297 397 378
NcoI C'CATG_G
Cuts at: 513 1065 3327 4081 513
Size: 552 2262 754 3504
Fragments arranged by size:
3504 2262 754 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7072
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7072
NotI GC'GGCC_GC
Cuts at: 2014 4519 2014
Size: 2505 4567
NspI r_CATG'y
Cuts at: 1132 1229 2668 3364 4430 5975 1132
Size: 97 1439 696 1066 1545 2229
Fragments arranged by size:
2229 1545 1439 1066 696 97
PasI CC'CwG_GG
Cuts at: 1348 3130 1348
Size: 1782 5290
PciI A'CATG_T
Cuts at: 2664 5971 2664
Size: 3307 3765
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7072
PfoI T'CCnGG_A
Cuts at: 2838 3012 3225 2838
Size: 174 213 6685
Fragments arranged by size:
6685 213 174
PmeI GTTT'AAAC
Cuts at: 4376 4376
Size: 7072
PmlI CAC'GTG
Cuts at: 2238 3003 2238
Size: 765 6307
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5141 5173 6654 6686 5141
Size: 32 1481 32 5527
Fragments arranged by size:
5527 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3433 3971 3433
Size: 538 6534
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7072
PsiI TTA'TAA
Cuts at: 4642 4642
Size: 7072
PspOMI G'GGCC_C
Cuts at: 3092 3092
Size: 7072
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2651 2683 2651
Size: 32 7040
PstI C_TGCA'G
Cuts at: 830 2268 3550 4167 4424 830
Size: 1438 1282 617 257 3478
Fragments arranged by size:
3478 1438 1282 617 257
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7072
PvuII CAG'CTG
Cuts at: 3421 3748 3829 4018 4174 3421
Size: 327 81 189 156 6319
Fragments arranged by size:
6319 327 189 156 81
RsrII CG'GwC_CG
Cuts at: 2353 2353
Size: 7072
SacI G_AGCT'C
Cuts at: 2907 2907
Size: 7072
SalI G'TCGA_C
Cuts at: 1936 4414 1936
Size: 2478 4594
SapI GCTCTTCn'nnn_
Cuts at: 4349 4349
Size: 7072
SbfI CC_TGCA'GG
Cuts at: 4424 4424
Size: 7072
ScaI AGT'ACT
Cuts at: 2792 5320 7032 2792
Size: 2528 1712 2832
Fragments arranged by size:
2832 2528 1712
SfcI C'TryA_G
Cuts at: 826 1045 2264 3546 3793 4163 4420 5555 6236
Size: 219 1219 1282 247 370 257 1135 681
Cuts at: 6236 6427 826
Size: 191 1471
Fragments arranged by size:
1471 1282 1219 1135 681 370 257 247
219 191
SfoI GGC'GCC
Cuts at: 2088 2466 2863 2088
Size: 378 397 6297
Fragments arranged by size:
6297 397 378
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7072
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7072
SmaI CCC'GGG
Cuts at: 1872 2143 4399 1872
Size: 271 2256 4545
Fragments arranged by size:
4545 2256 271
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5176 6077 6339 820
Size: 197 926 3233 901 262 1553
Fragments arranged by size:
3233 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4863 493
Size: 4370 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7072
SphI G_CATG'C
Cuts at: 1132 4430 1132
Size: 3298 3774
SrfI GCCC'GGGC
Cuts at: 2143 2143
Size: 7072
SspI AAT'ATT
Cuts at: 5 52 4996 5
Size: 47 4944 2081
Fragments arranged by size:
4944 2081 47
StuI AGG'CCT
Cuts at: 1517 3110 1517
Size: 1593 5479
StyI C'CwwG_G
Cuts at: 513 1065 2985 3327 4081 4311 4511 513
Size: 552 1920 342 754 230 200 3074
Fragments arranged by size:
3074 1920 754 552 342 230 200
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2482 3011 5099 5116 5269 5454 5899
Size: 1255 902 529 2088 17 153 185 445
Cuts at: 5899 325
Size: 1498
Fragments arranged by size:
2088 1498 1255 902 529 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2790 3603 5318
Size: 274 80 33 51 1443 813 813 1715
Cuts at: 5318 7030 96
Size: 1712 138
Fragments arranged by size:
1715 1712 1443 813 813 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4292 4324 7032 7064 4292
Size: 32 2708 32 4300
Fragments arranged by size:
4300 2708 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3233 1316
Size: 1917 5155
XbaI T'CTAG_A
Cuts at: 2716 4408 2716
Size: 1692 5380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4243 4243
Size: 7072
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7072
XmaI C'CCGG_G
Cuts at: 1870 2141 4397 1870
Size: 271 2256 4545
Fragments arranged by size:
4545 2256 271
XmnI GAAnn'nnTTC
Cuts at: 5201 5201
Size: 7072
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4880 276
Size: 53 83 186 936 3346 2468
Fragments arranged by size:
3346 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoRV FseI NaeI NgoMIV
NruI NsiI PacI PspXI SacII SanDI SexAI SfiI
SwaI