Restriction map for
pFN21AA1953
(Circular) (Six-base) MAPSORT of: pFN21AA1953.seq Check: 5214 from: 1 to: 7531
pFN21AA1953 7531 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3588 3588
Size: 7531
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2796 5341 278
Size: 53 83 186 936 1260 2545 2468
Fragments arranged by size:
2545 2468 1260 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2177 4874 1937
Size: 240 2697 4594
Fragments arranged by size:
4594 2697 240
Acc65I G'GTAC_C
Cuts at: 4852 4852
Size: 7531
AclI AA'CG_TT
Cuts at: 1563 5658 6031 1563
Size: 4095 373 3063
Fragments arranged by size:
4095 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2259 3319 3647 4595 5594 6978 7246 1632
Size: 627 1060 328 948 999 1384 268 1917
Fragments arranged by size:
1917 1384 1060 999 948 627 328 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7334
AflIII A'CryG_T
Cuts at: 1203 6430 7407 1203
Size: 5227 977 1327
Fragments arranged by size:
5227 1327 977
AgeI A'CCGG_T
Cuts at: 3943 6362 3943
Size: 2419 5112
AhdI GACnn_n'nnGTC
Cuts at: 2105 6260 2105
Size: 4155 3376
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7531
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2546 2871 4241 1184
Size: 1362 325 1370 4474
Fragments arranged by size:
4474 1370 1362 325
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2240 2272 2240
Size: 32 7499
AlwNI CAG_nnn'CTG
Cuts at: 2137 2545 3826 4515 4540 5274 6846 2137
Size: 408 1281 689 25 734 1572 2822
Fragments arranged by size:
2822 1572 1281 734 689 408 25
ApaI G_GGCC'C
Cuts at: 3133 3820 4581 3133
Size: 687 761 6083
Fragments arranged by size:
6083 761 687
ApaLI G'TGCA_C
Cuts at: 2846 3386 5588 6744 2846
Size: 540 2202 1156 3633
Fragments arranged by size:
3633 2202 1156 540
ApoI r'AATT_y
Cuts at: 1493 2162 2763 3614 4840 5066 5218 7327 1493
Size: 669 601 851 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 851 669 601 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6085 160
Size: 872 5053 1606
Fragments arranged by size:
5053 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7531
AvaI C'yCGr_G
Cuts at: 1870 1943 2110 3273 3285 4112 4271 4856 1870
Size: 73 167 1163 12 827 159 585 4545
Fragments arranged by size:
4545 1163 827 585 167 159 73 12
BamHI G'GATC_C
Cuts at: 4861 5228 4861
Size: 367 7164
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2499 4183 4453 4852 6307
Size: 325 217 627 712 1684 270 399 1455
Cuts at: 6307 618
Size: 1842
Fragments arranged by size:
1842 1684 1455 712 627 399 325 270
217
BanII G_rGCy'C
Cuts at: 1391 3133 3767 3820 4412 4581 4830 4850 1391
Size: 1742 634 53 592 169 249 20 4072
Fragments arranged by size:
4072 1742 634 592 249 169 53 20
BbeI G_GCGC'C
Cuts at: 2503 4457 2503
Size: 1954 5577
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2247 3202 3635 4572 928
Size: 971 348 955 433 937 3887
Fragments arranged by size:
3887 971 955 937 433 348
BbvCI CC'TCA_GC
Cuts at: 3872 3872
Size: 7531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5722 5756 1903
Size: 34 50 34 3701 34 3678
Fragments arranged by size:
3701 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5423 6633 5423
Size: 1210 6321
BclI T'GATC_A
Cuts at: 1548 4138 1548
Size: 2590 4941
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3646 6142 136
Size: 107 122 71 631 605 1974 2496 1525
Fragments arranged by size:
2496 1974 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4262 7526 4262
Size: 3264 4267
BlpI GC'TnA_GC
Cuts at: 2558 3761 4948 2558
Size: 1203 1187 5141
Fragments arranged by size:
5141 1203 1187
Bme1580I G_kGCm'C
Cuts at: 1790 2850 3133 3390 3820 4186 4581 5592 6748
Size: 1060 283 257 430 366 395 1011 1156
Cuts at: 6748 1790
Size: 2573
Fragments arranged by size:
2573 1156 1060 1011 430 395 366 283
257
BmgBI CAC'GTC
Cuts at: 1318 2569 3328 1318
Size: 1251 759 5521
Fragments arranged by size:
5521 1251 759
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4658 5262 6220
Size: 416 53 83 399 24 3241 604 958
Cuts at: 6220 442
Size: 1753
Fragments arranged by size:
3241 1753 958 604 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7531
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 2624 3042 3129 3365 3380 3711 3754
Size: 1361 24 418 87 236 15 331 43
Cuts at: 3754 3923 4382 6191 1239
Size: 169 459 1809 2579
Fragments arranged by size:
2579 1809 1361 459 418 331 236 169
87 43 24 15
Bpu10I CC'TnA_GC
Cuts at: 3872 4073 3872
Size: 201 7330
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3239 3977 5656 6521 6819 3239
Size: 738 1679 865 298 3951
Fragments arranged by size:
3951 1679 865 738 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3180 4138 6194 882
Size: 617 1681 958 2056 2219
Fragments arranged by size:
2219 2056 1681 958 617
BsaAI yAC'GTr
Cuts at: 493 1204 3166 3586 5322 493
Size: 711 1962 420 1736 2702
Fragments arranged by size:
2702 1962 1736 711 420
BsaBI GATnn'nnATC
Cuts at: 4044 7525 4044
Size: 3481 4050
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2500 2793 4454 5338
Size: 53 83 186 936 967 293 1661 884
Cuts at: 5338 5720 6355 275
Size: 382 635 1451
Fragments arranged by size:
1661 1451 967 936 884 635 382 293
186 83 53
BsaWI w'CCGG_w
Cuts at: 3018 3189 3790 3943 5964 6362 6636 6783 7194
Size: 171 601 153 2021 398 274 147 411
Cuts at: 7194 3018
Size: 3355
Fragments arranged by size:
3355 2021 601 411 398 274 171 153
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2304 2334 3338 3368 4305
Size: 30 165 30 901 30 1004 30 937
Cuts at: 4305 4335 1178
Size: 30 4374
Fragments arranged by size:
4374 1004 937 901 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2498 3143 3407 3410 3440 3920 3978 1186
Size: 1312 645 264 3 30 480 58 4739
Fragments arranged by size:
4739 1312 645 480 264 58 30 3
BseYI C'CCAG_C
Cuts at: 2017 2073 2447 2538 3479 4353 4528 4580 6734
Size: 56 374 91 941 874 175 52 2154
Cuts at: 6734 7230 2017
Size: 496 2318
Fragments arranged by size:
2318 2154 941 874 496 374 175 91
56 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3512 3566 3971 1163
Size: 573 1776 54 405 4723
Fragments arranged by size:
4723 1776 573 405 54
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3237 4981 5742 6770 1540
Size: 275 186 1236 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1236 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2520 2850 3390 3767 4323 4830 5592 5677 6748
Size: 330 540 377 556 507 762 85 1071
Cuts at: 6748 2520
Size: 3303
Fragments arranged by size:
3303 1071 762 556 540 507 377 330
85
BsmI GAATG_Cn'
Cuts at: 2226 5042 5135 2226
Size: 2816 93 4622
Fragments arranged by size:
4622 2816 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3236 3734 3236
Size: 498 7033
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2520 2850 3133 3390 3767 3820 4186
Size: 399 730 330 283 257 377 53 366
Cuts at: 4186 4323 4412 4581 4830 4850 5592 5677 6748
Size: 137 89 169 249 20 742 85 1071
Cuts at: 6748 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 730 399 377 366 330
283 257 249 169 137 89 85 53
20
BspEI T'CCGG_A
Cuts at: 7194 7194
Size: 7531
BspHI T'CATG_A
Cuts at: 5420 7150 5420
Size: 1730 5801
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3588 4886 844
Size: 2744 1298 3489
Fragments arranged by size:
3489 2744 1298
BsrBI CCG'CTC
Cuts at: 1394 1823 3448 3802 5418 1394
Size: 429 1625 354 1616 3507
Fragments arranged by size:
3507 1625 1616 429 354
BsrDI GCAATG_nn'
Cuts at: 65 1248 3543 4485 6026 6200 65
Size: 1183 2295 942 1541 174 1396
Fragments arranged by size:
2295 1541 1396 1183 942 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3880 3943 4450 6175 6362 1700
Size: 110 2070 63 507 1725 187 2869
Fragments arranged by size:
2869 2070 1725 507 187 110 63
BsrGI T'GTAC_A
Cuts at: 96 3084 96
Size: 2988 4543
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7531
BssSI C'ACGA_G
Cuts at: 3389 5591 6603 3389
Size: 2202 1012 4317
Fragments arranged by size:
4317 2202 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3958 794
Size: 3164 4367
BstBI TT'CG_AA
Cuts at: 6335 6335
Size: 7531
BstEII G'GTnAC_C
Cuts at: 1184 3592 1184
Size: 2408 5123
BstXI CCAn_nnnn'nTGG
Cuts at: 2890 3487 4671 2890
Size: 597 1184 5750
Fragments arranged by size:
5750 1184 597
BstYI r'GATC_y
Cuts at: 1972 3258 4262 4861 5228 5613 5630 7082 7168
Size: 1286 1004 599 367 385 17 1452 86
Cuts at: 7168 7180 7526 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1286 1004 599 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4124 4629 4124
Size: 505 7026
BtgI C'CryG_G
Cuts at: 513 1065 3231 3913 3930 4519 513
Size: 552 2166 682 17 589 3525
Fragments arranged by size:
3525 2166 682 589 552 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2578 3711 487
Size: 683 56 1352 1133 4307
Fragments arranged by size:
4307 1352 1133 683 56
BtsI GCAGTG_nn'
Cuts at: 2445 2647 2917 5047 5841 5861 2445
Size: 202 270 2130 794 20 4115
Fragments arranged by size:
4115 2130 794 270 202 20
ClaI AT'CG_AT
Cuts at: 1554 3468 1554
Size: 1914 5617
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7496
DraI TTT'AAA
Cuts at: 2722 4835 2722
Size: 2113 5418
DraIII CAC_nnn'GTG
Cuts at: 4089 4089
Size: 7531
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4154 4530 6538 809
Size: 507 2838 376 2008 1802
Fragments arranged by size:
2838 2008 1802 507 376
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4201 4807 4978
Size: 54 1425 50 275 18 2371 606 171
Cuts at: 4978 5867 7496 8
Size: 889 1629 43
Fragments arranged by size:
2371 1629 1425 889 606 275 171 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4978 1537
Size: 275 3166 4090
Fragments arranged by size:
4090 3166 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2089 2348 2963 4695 5461 1353
Size: 702 34 259 615 1732 766 3423
Fragments arranged by size:
3423 1732 766 702 615 259 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6118 6492 6638 387
Size: 1064 138 4529 374 146 1280
Fragments arranged by size:
4529 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3765 4828 3765
Size: 1063 6468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2259 2600 2624 3042 3129 3319 3365
Size: 393 627 341 24 418 87 190 46
Cuts at: 3365 3380 3647 3711 3754 3923 4382 4595 5594
Size: 15 267 64 43 169 459 213 999
Cuts at: 5594 6191 6978 7246 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 999 787 627 597 459 418 393
341 268 267 213 190 169 87 64
46 43 24 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3905 4544 3905
Size: 639 6892
EcoO109I rG'GnC_Cy
Cuts at: 2107 3129 3130 3441 3823 3935 3983 4501 4577
Size: 1022 1 311 382 112 48 518 76
Cuts at: 4577 4625 2107
Size: 48 5013
Fragments arranged by size:
5013 1022 518 382 311 112 76 48
48 1
EcoRI G'AATT_C
Cuts at: 2162 4840 5218 2162
Size: 2678 378 4475
Fragments arranged by size:
4475 2678 378
EcoRV GAT'ATC
Cuts at: 3048 3048
Size: 7531
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3058 3058
Size: 7531
FspI TGC'GCA
Cuts at: 4229 6037 4229
Size: 1808 5723
HaeII r_GCGC'y
Cuts at: 1678 2503 4457 6678 1678
Size: 825 1954 2221 2531
Fragments arranged by size:
2531 2221 1954 825
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2091 2123 2763 2778 2795
Size: 32 135 32 519 32 640 15 17
Cuts at: 2795 2810 3279 3311 3338 3370 3392 3424 3975
Size: 15 469 32 27 32 22 32 551
Cuts at: 3975 4007 4252 4254 4284 4286 4305 4337 6172
Size: 32 245 2 30 2 19 32 1835
Cuts at: 6172 6204 6246 6278 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1835 640 551 519 469 245 135
42 32 32 32 32 32 32 32
32 32 32 30 27 22 19 17
15 15 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4875 5121 669
Size: 988 281 2937 246 3079
Fragments arranged by size:
3079 2937 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7531
HpaI GTT'AAC
Cuts at: 5121 5121
Size: 7531
KasI G'GCGC_C
Cuts at: 2499 4453 2499
Size: 1954 5577
KpnI G_GTAC'C
Cuts at: 4856 4856
Size: 7531
MfeI C'AATT_G
Cuts at: 5130 5130
Size: 7531
MluI A'CGCG_T
Cuts at: 7407 7407
Size: 7531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3004 3039 6645 6829 1305
Size: 1699 35 3606 184 2007
Fragments arranged by size:
3606 2007 1699 184 35
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4203 4809 10
Size: 54 1425 343 2371 606 2732
Fragments arranged by size:
2732 2371 1425 606 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 3863 4796 5489 5848 6007 518
Size: 648 1362 1335 933 693 359 159 2042
Fragments arranged by size:
2042 1362 1335 933 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2276 2545 3814 4947 5624 6772 7017 1606
Size: 670 269 1269 1133 677 1148 245 2120
Fragments arranged by size:
2120 1269 1148 1133 677 670 269 245
NaeI GCC'GGC
Cuts at: 4452 4452
Size: 7531
NarI GG'CG_CC
Cuts at: 2500 4454 2500
Size: 1954 5577
NcoI C'CATG_G
Cuts at: 513 1065 3930 513
Size: 552 2865 4114
Fragments arranged by size:
4114 2865 552
NdeI CA'TA_TG
Cuts at: 387 3096 387
Size: 2709 4822
NgoMIV G'CCGG_C
Cuts at: 4450 4450
Size: 7531
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7531
NotI GC'GGCC_GC
Cuts at: 4978 4978
Size: 7531
NspI r_CATG'y
Cuts at: 1132 1229 3922 4889 6434 1132
Size: 97 2693 967 1545 2229
Fragments arranged by size:
2693 2229 1545 967 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7531
PciI A'CATG_T
Cuts at: 6430 6430
Size: 7531
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3826 4339 1108
Size: 2718 513 4300
Fragments arranged by size:
4300 2718 513
PfoI T'CCnGG_A
Cuts at: 2549 2577 2549
Size: 28 7503
PmeI GTTT'AAAC
Cuts at: 4835 4835
Size: 7531
PmlI CAC'GTG
Cuts at: 3586 3586
Size: 7531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5600 5632 7113 7145 5600
Size: 32 1481 32 5986
Fragments arranged by size:
5986 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2107 3441 3935 3983 2107
Size: 1334 494 48 5655
Fragments arranged by size:
5655 1334 494 48
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7531
PsiI TTA'TAA
Cuts at: 5101 5101
Size: 7531
PspOMI G'GGCC_C
Cuts at: 3129 3816 4577 3129
Size: 687 761 6083
Fragments arranged by size:
6083 761 687
PstI C_TGCA'G
Cuts at: 830 2219 2281 2440 2496 3138 4576 4883 830
Size: 1389 62 159 56 642 1438 307 3478
Fragments arranged by size:
3478 1438 1389 642 307 159 62 56
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7531
PvuII CAG'CTG
Cuts at: 2276 2545 2276
Size: 269 7262
SacI G_AGCT'C
Cuts at: 3767 4830 3767
Size: 1063 6468
SalI G'TCGA_C
Cuts at: 1936 4873 1936
Size: 2937 4594
SanDI GG'GwC_CC
Cuts at: 2107 3935 2107
Size: 1828 5703
SapI GCTCTTCn'nnn_
Cuts at: 4695 4695
Size: 7531
SbfI CC_TGCA'GG
Cuts at: 4576 4883 4576
Size: 307 7224
ScaI AGT'ACT
Cuts at: 5779 7491 5779
Size: 1712 5819
SfcI C'TryA_G
Cuts at: 826 1045 2215 2277 2436 2492 2730 3134 4572
Size: 219 1170 62 159 56 238 404 1438
Cuts at: 4572 4879 6014 6695 6886 826
Size: 307 1135 681 191 1471
Fragments arranged by size:
1471 1438 1170 1135 681 404 307 238
219 191 159 62 56
SfoI GGC'GCC
Cuts at: 2501 4455 2501
Size: 1954 5577
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7531
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7531
SmaI CCC'GGG
Cuts at: 1872 4114 4858 1872
Size: 2242 744 4545
Fragments arranged by size:
4545 2242 744
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3254 3992 4271 5635 6536 6798
Size: 197 926 1311 738 279 1364 901 262
Cuts at: 6798 820
Size: 1553
Fragments arranged by size:
1553 1364 1311 926 901 738 279 262
197
SnaBI TAC'GTA
Cuts at: 493 5322 493
Size: 4829 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7531
SphI G_CATG'C
Cuts at: 1132 4889 1132
Size: 3757 3774
SspI AAT'ATT
Cuts at: 5 52 2424 5455 5
Size: 47 2372 3031 2081
Fragments arranged by size:
3031 2372 2081 47
StuI AGG'CCT
Cuts at: 1517 3688 1517
Size: 2171 5360
StyI C'CwwG_G
Cuts at: 513 1065 2946 3930 4970 513
Size: 552 1881 984 1040 3074
Fragments arranged by size:
3074 1881 1040 984 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3118 5558 5575 5728 5913 6358 325
Size: 1255 1538 2440 17 153 185 445 1498
Fragments arranged by size:
2440 1538 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2734 3084 4758
Size: 274 80 33 51 1443 757 350 1674
Cuts at: 4758 5777 7489 96
Size: 1019 1712 138
Fragments arranged by size:
1712 1674 1443 1019 757 350 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2304 2336 7491 7523 2304
Size: 32 5155 32 2312
Fragments arranged by size:
5155 2312 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7531
XbaI T'CTAG_A
Cuts at: 4867 4867
Size: 7531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2831 3179 2831
Size: 348 7183
XhoI C'TCGA_G
Cuts at: 1943 4271 1943
Size: 2328 5203
XmaI C'CCGG_G
Cuts at: 1870 4112 4856 1870
Size: 2242 744 4545
Fragments arranged by size:
4545 2242 744
XmnI GAAnn'nnTTC
Cuts at: 5660 5660
Size: 7531
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2794 5339 276
Size: 53 83 186 936 1260 2545 2468
Fragments arranged by size:
2545 2468 1260 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacII SexAI SfiI SrfI SwaI