Restriction map for
pFN21AA1936
(Circular) (Six-base) MAPSORT of: pFN21AA1936.seq Check: 3355 from: 1 to: 7117
pFN21AA1936 7117 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3565 3565
Size: 7117
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2069 4927 278
Size: 53 83 186 936 533 2858 2468
Fragments arranged by size:
2858 2468 936 533 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4460 1937
Size: 2523 4594
Acc65I G'GTAC_C
Cuts at: 4438 4438
Size: 7117
AclI AA'CG_TT
Cuts at: 1563 5244 5617 1563
Size: 3681 373 3063
Fragments arranged by size:
3681 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3485 5180 6564 6832 1632
Size: 1853 1695 1384 268 1917
Fragments arranged by size:
1917 1853 1695 1384 268
AfeI AGC'GCT
Cuts at: 2664 2664
Size: 7117
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6920
AflIII A'CryG_T
Cuts at: 1203 2316 2375 3489 6016 6993 1203
Size: 1113 59 1114 2527 977 1327
Fragments arranged by size:
2527 1327 1114 1113 977 59
AgeI A'CCGG_T
Cuts at: 5948 5948
Size: 7117
AhdI GACnn_n'nnGTC
Cuts at: 3006 5846 3006
Size: 2840 4277
AleI CACnn'nnGTG
Cuts at: 1166 3644 1166
Size: 2478 4639
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2808 3593 3877 1184
Size: 1624 785 284 4424
Fragments arranged by size:
4424 1624 785 284
AlwNI CAG_nnn'CTG
Cuts at: 2264 2563 3769 4042 4860 6432 2264
Size: 299 1206 273 818 1572 2949
Fragments arranged by size:
2949 1572 1206 818 299 273
ApaI G_GGCC'C
Cuts at: 3843 3843
Size: 7117
ApaLI G'TGCA_C
Cuts at: 5174 6330 5174
Size: 1156 5961
ApoI r'AATT_y
Cuts at: 1493 2237 3323 4426 4652 4804 6913 1493
Size: 744 1086 1103 226 152 2109 1697
Fragments arranged by size:
2109 1697 1103 1086 744 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3246 5671 160
Size: 872 2214 2425 1606
Fragments arranged by size:
2425 2214 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7117
AvaI C'yCGr_G
Cuts at: 1870 1943 4122 4442 1870
Size: 73 2179 320 4545
Fragments arranged by size:
4545 2179 320 73
AvrII C'CTAG_G
Cuts at: 3187 3187
Size: 7117
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2396 2429 2396
Size: 33 7084
BamHI G'GATC_C
Cuts at: 4447 4814 4447
Size: 367 6750
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4050 4438 5893 618
Size: 325 217 627 2263 388 1455 1842
Fragments arranged by size:
2263 1842 1455 627 388 325 217
BanII G_rGCy'C
Cuts at: 1391 2778 2991 3843 4236 4359 4436 1391
Size: 1387 213 852 393 123 77 4072
Fragments arranged by size:
4072 1387 852 393 213 123 77
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3271 928
Size: 971 1372 4774
Fragments arranged by size:
4774 1372 971
BbvCI CC'TCA_GC
Cuts at: 2666 3770 4305 2666
Size: 1104 535 5478
Fragments arranged by size:
5478 1104 535
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5308 5342 1903
Size: 34 3371 34 3678
Fragments arranged by size:
3678 3371 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2424 5009 6219 2424
Size: 2585 1210 3322
Fragments arranged by size:
3322 2585 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7117
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5728 136
Size: 107 122 71 631 605 4056 1525
Fragments arranged by size:
4056 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3917 4251 7112 3917
Size: 334 2861 3922
Fragments arranged by size:
3922 2861 334
BlpI GC'TnA_GC
Cuts at: 4534 4534
Size: 7117
Bme1580I G_kGCm'C
Cuts at: 1790 3843 5178 6334 1790
Size: 2053 1335 1156 2573
Fragments arranged by size:
2573 2053 1335 1156
BmgBI CAC'GTC
Cuts at: 1318 2376 3457 3898 1318
Size: 1058 1081 441 4537
Fragments arranged by size:
4537 1081 1058 441
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3044 4848 5806
Size: 416 53 83 399 24 1627 1804 958
Cuts at: 5806 442
Size: 1753
Fragments arranged by size:
1804 1753 1627 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3084 3084
Size: 7117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4343 5777 1239
Size: 3104 1434 2579
Fragments arranged by size:
3104 2579 1434
Bpu10I CC'TnA_GC
Cuts at: 2666 3513 3770 4286 4305 2666
Size: 847 257 516 19 5478
Fragments arranged by size:
5478 847 516 257 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2122 2672 5242 6107 6405 2122
Size: 550 2570 865 298 2834
Fragments arranged by size:
2834 2570 865 550 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2090 3215 3780 3961 5780 882
Size: 617 591 1125 565 181 1819 2219
Fragments arranged by size:
2219 1819 1125 617 591 565 181
BsaAI yAC'GTr
Cuts at: 493 1204 2447 4908 493
Size: 711 1243 2461 2702
Fragments arranged by size:
2702 2461 1243 711
BsaBI GATnn'nnATC
Cuts at: 7111 7111
Size: 7117
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 3680 4062 4924
Size: 53 83 186 936 533 1614 382 862
Cuts at: 4924 5306 5941 275
Size: 382 635 1451
Fragments arranged by size:
1614 1451 936 862 635 533 382 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5550 5948 6222 6369 6780 5550
Size: 398 274 147 411 5887
Fragments arranged by size:
5887 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2253 2283 3882 3912 4301
Size: 30 165 30 850 30 1599 30 389
Cuts at: 4301 4331 1178
Size: 30 3964
Fragments arranged by size:
3964 1599 850 389 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2113 2196 2687 3403 4189 4322 1186
Size: 927 83 491 716 786 133 3981
Fragments arranged by size:
3981 927 786 716 491 133 83
BseYI C'CCAG_C
Cuts at: 2629 6320 6816 2629
Size: 3691 496 2930
Fragments arranged by size:
3691 2930 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2804 3333 3376 3945 4307 1163
Size: 573 1068 529 43 569 362 3973
Fragments arranged by size:
3973 1068 573 569 529 362 43
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4567 5328 6356 1540
Size: 275 186 2566 761 1028 2301
Fragments arranged by size:
2566 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2778 2991 3321 3337 4089 4236 4292 4359 5178
Size: 213 330 16 752 147 56 67 819
Cuts at: 5178 5263 6334 2778
Size: 85 1071 3561
Fragments arranged by size:
3561 1071 819 752 330 213 147 85
67 56 16
BsmI GAATG_Cn'
Cuts at: 3854 4628 4721 3854
Size: 774 93 6250
Fragments arranged by size:
6250 774 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2366 2366
Size: 7117
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2778 2991 3321 3337 3843 4089 4236
Size: 399 988 213 330 16 506 246 147
Cuts at: 4236 4292 4359 4436 5178 5263 6334 1391
Size: 56 67 77 742 85 1071 2174
Fragments arranged by size:
2174 1071 988 742 506 399 330 246
213 147 85 77 67 56 16
BspEI T'CCGG_A
Cuts at: 6780 6780
Size: 7117
BspHI T'CATG_A
Cuts at: 5006 6736 5006
Size: 1730 5387
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3565 3973 4472 844
Size: 2721 408 499 3489
Fragments arranged by size:
3489 2721 499 408
BsrBI CCG'CTC
Cuts at: 1394 1823 2340 5004 1394
Size: 429 517 2664 3507
Fragments arranged by size:
3507 2664 517 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3051 5612 5786 65
Size: 1183 1803 2561 174 1396
Fragments arranged by size:
2561 1803 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5761 5948 1700
Size: 110 3951 187 2869
Fragments arranged by size:
3951 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7117
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7117
BssSI C'ACGA_G
Cuts at: 5177 6189 5177
Size: 1012 6105
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3870 794
Size: 3076 4041
BstBI TT'CG_AA
Cuts at: 5921 5921
Size: 7117
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7117
BstXI CCAn_nnnn'nTGG
Cuts at: 3722 3722
Size: 7117
BstYI r'GATC_y
Cuts at: 1972 2008 2722 3917 4251 4447 4814 5199 5216
Size: 36 714 1195 334 196 367 385 17
Cuts at: 5216 6668 6754 6766 7112 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1195 714 385 367 346 334
196 86 36 17 12
Bsu36I CC'TnA_GG
Cuts at: 2142 3059 4138 2142
Size: 917 1079 5121
Fragments arranged by size:
5121 1079 917
BtgI C'CryG_G
Cuts at: 513 1065 2004 4092 4201 4334 513
Size: 552 939 2088 109 133 3296
Fragments arranged by size:
3296 2088 939 552 133 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3491 3533 487
Size: 683 56 2265 42 4071
Fragments arranged by size:
4071 2265 683 56 42
BtsI GCAGTG_nn'
Cuts at: 2274 3438 3715 4633 5427 5447 2274
Size: 1164 277 918 794 20 3944
Fragments arranged by size:
3944 1164 918 794 277 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7117
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7082
DraI TTT'AAA
Cuts at: 2730 4421 2730
Size: 1691 5426
DraIII CAC_nnn'GTG
Cuts at: 3038 4093 3038
Size: 1055 6062
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2867 3953 6124 809
Size: 507 1551 1086 2171 1802
Fragments arranged by size:
2171 1802 1551 1086 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2640 2916 4564
Size: 54 1425 50 275 18 810 276 1648
Cuts at: 4564 5453 7082 8
Size: 889 1629 43
Fragments arranged by size:
1648 1629 1425 889 810 276 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4564 1537
Size: 275 2752 4090
Fragments arranged by size:
4090 2752 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2131 2357 4241 5047 1353
Size: 778 226 1884 806 3423
Fragments arranged by size:
3423 1884 806 778 226
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3922 5704 6078 6224 387
Size: 1064 138 2333 1782 374 146 1280
Fragments arranged by size:
2333 1782 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2776 2989 4234 4357 2776
Size: 213 1245 123 5536
Fragments arranged by size:
5536 1245 213 123
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3485 4343 5180 5777 6564 6832 1239
Size: 393 1853 858 837 597 787 268 1524
Fragments arranged by size:
1853 1524 858 837 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2730 2783 3015 2730
Size: 53 232 6832
Fragments arranged by size:
6832 232 53
EcoO109I rG'GnC_Cy
Cuts at: 2881 3542 3839 3970 4406 2881
Size: 661 297 131 436 5592
Fragments arranged by size:
5592 661 436 297 131
EcoRI G'AATT_C
Cuts at: 4426 4804 4426
Size: 378 6739
EcoRV GAT'ATC
Cuts at: 2110 2110
Size: 7117
FspI TGC'GCA
Cuts at: 5623 5623
Size: 7117
HaeII r_GCGC'y
Cuts at: 1678 2666 3881 6264 1678
Size: 988 1215 2383 2531
Fragments arranged by size:
2531 2383 1215 988
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2015 2047 2303 2335 2992
Size: 32 135 32 443 32 256 32 657
Cuts at: 2992 3024 4301 4333 5758 5790 5832 5864 1373
Size: 32 1277 32 1425 32 42 32 2626
Fragments arranged by size:
2626 1425 1277 657 443 256 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3066 4461 4707 669
Size: 988 281 1128 1395 246 3079
Fragments arranged by size:
3079 1395 1128 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2623 3110 4001 748
Size: 1875 487 891 3864
Fragments arranged by size:
3864 1875 891 487
HpaI GTT'AAC
Cuts at: 4707 4707
Size: 7117
KpnI G_GTAC'C
Cuts at: 4442 4442
Size: 7117
MfeI C'AATT_G
Cuts at: 4716 4716
Size: 7117
MluI A'CGCG_T
Cuts at: 6993 6993
Size: 7117
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4362 6231 6415 1305
Size: 3057 1869 184 2007
Fragments arranged by size:
3057 2007 1869 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2642 2918 10
Size: 54 1425 343 810 276 4209
Fragments arranged by size:
4209 1425 810 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3644 3720 5075 5434 5593 518
Size: 648 2478 76 1355 359 159 2042
Fragments arranged by size:
2478 2042 1355 648 359 159 76
MspA1I CmG'CkG
Cuts at: 1606 3688 4039 4533 5210 6358 6603 1606
Size: 2082 351 494 677 1148 245 2120
Fragments arranged by size:
2120 2082 1148 677 494 351 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 4334 513
Size: 552 939 2330 3296
Fragments arranged by size:
3296 2330 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7117
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7117
NotI GC'GGCC_GC
Cuts at: 4564 4564
Size: 7117
NspI r_CATG'y
Cuts at: 1132 1229 2320 3493 4475 6020 1132
Size: 97 1091 1173 982 1545 2229
Fragments arranged by size:
2229 1545 1173 1091 982 97
PasI CC'CwG_GG
Cuts at: 1348 2470 1348
Size: 1122 5995
PciI A'CATG_T
Cuts at: 2316 3489 6016 2316
Size: 1173 2527 3417
Fragments arranged by size:
3417 2527 1173
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3233 1108
Size: 2125 4992
PfoI T'CCnGG_A
Cuts at: 3232 4350 3232
Size: 1118 5999
PmeI GTTT'AAAC
Cuts at: 4421 4421
Size: 7117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5186 5218 6699 6731 5186
Size: 32 1481 32 5572
Fragments arranged by size:
5572 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2881 3542 3970 2881
Size: 661 428 6028
Fragments arranged by size:
6028 661 428
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7117
PsiI TTA'TAA
Cuts at: 3270 4687 3270
Size: 1417 5700
PspOMI G'GGCC_C
Cuts at: 3839 3839
Size: 7117
PstI C_TGCA'G
Cuts at: 830 2269 2484 2568 3838 4469 830
Size: 1439 215 84 1270 631 3478
Fragments arranged by size:
3478 1439 1270 631 215 84
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7117
SacI G_AGCT'C
Cuts at: 2778 2991 4236 4359 2778
Size: 213 1245 123 5536
Fragments arranged by size:
5536 1245 213 123
SalI G'TCGA_C
Cuts at: 1936 4459 1936
Size: 2523 4594
SapI GCTCTTCn'nnn_
Cuts at: 4241 4241
Size: 7117
SbfI CC_TGCA'GG
Cuts at: 4469 4469
Size: 7117
ScaI AGT'ACT
Cuts at: 5365 7077 5365
Size: 1712 5405
SexAI A'CCwGG_T
Cuts at: 3951 3951
Size: 7117
SfcI C'TryA_G
Cuts at: 826 1045 2088 2215 2265 2480 2564 3628 3834
Size: 219 1043 127 50 215 84 1064 206
Cuts at: 3834 3974 4169 4401 4465 5600 6281 6472 826
Size: 140 195 232 64 1135 681 191 1471
Fragments arranged by size:
1471 1135 1064 1043 681 232 219 215
206 195 191 140 127 84 64 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7117
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7117
SmaI CCC'GGG
Cuts at: 1872 4124 4444 1872
Size: 2252 320 4545
Fragments arranged by size:
4545 2252 320
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2101 2651 5221 6122 6384 820
Size: 197 926 158 550 2570 901 262 1553
Fragments arranged by size:
2570 1553 926 901 550 262 197 158
SnaBI TAC'GTA
Cuts at: 493 2447 4908 493
Size: 1954 2461 2702
Fragments arranged by size:
2702 2461 1954
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7117
SphI G_CATG'C
Cuts at: 1132 4475 1132
Size: 3343 3774
SrfI GCCC'GGGC
Cuts at: 4124 4124
Size: 7117
SspI AAT'ATT
Cuts at: 5 52 5041 5
Size: 47 4989 2081
Fragments arranged by size:
4989 2081 47
StuI AGG'CCT
Cuts at: 1517 2302 2981 1517
Size: 785 679 5653
Fragments arranged by size:
5653 785 679
StyI C'CwwG_G
Cuts at: 513 1065 2004 3187 4334 4556 513
Size: 552 939 1183 1147 222 3074
Fragments arranged by size:
3074 1183 1147 939 552 222
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3026 4178 4408 5144 5161 5314 5499
Size: 1255 1446 1152 230 736 17 153 185
Cuts at: 5499 5944 325
Size: 445 1498
Fragments arranged by size:
1498 1446 1255 1152 736 445 230 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 5363 7075
Size: 274 80 33 51 1443 297 3089 1712
Cuts at: 7075 96
Size: 138
Fragments arranged by size:
3089 1712 1443 297 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2253 2285 3882 3914 4193 4225 7077 7109 2253
Size: 32 1597 32 279 32 2852 32 2261
Fragments arranged by size:
2852 2261 1597 279 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7117
XbaI T'CTAG_A
Cuts at: 4453 4453
Size: 7117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4219 4219
Size: 7117
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7117
XmaI C'CCGG_G
Cuts at: 1870 4122 4442 1870
Size: 2252 320 4545
Fragments arranged by size:
4545 2252 320
XmnI GAAnn'nnTTC
Cuts at: 5246 5246
Size: 7117
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2067 4925 276
Size: 53 83 186 936 533 2858 2468
Fragments arranged by size:
2858 2468 936 533 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AloI AscI BbeI BfrBI BsiWI BstZ17I FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI PvuII RsrII SacII SanDI SfiI
SfoI SwaI