Restriction map for
pFN21AA1910
(Circular) (Six-base) MAPSORT of: pFN21AA1910.seq Check: 4551 from: 1 to: 6793
pFN21AA1910 6793 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2036 4603 278
Size: 53 83 186 936 500 2567 2468
Fragments arranged by size:
2567 2468 936 500 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2124 4136 1937
Size: 187 2012 4594
Fragments arranged by size:
4594 2012 187
Acc65I G'GTAC_C
Cuts at: 4114 4114
Size: 6793
AclI AA'CG_TT
Cuts at: 1563 2058 4920 5293 1563
Size: 495 2862 373 3063
Fragments arranged by size:
3063 2862 495 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4856 6240 6508 1632
Size: 3224 1384 268 1917
Fragments arranged by size:
3224 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6596
AflIII A'CryG_T
Cuts at: 1203 2119 2520 3471 5692 6669 1203
Size: 916 401 951 2221 977 1327
Fragments arranged by size:
2221 1327 977 951 916 401
AgeI A'CCGG_T
Cuts at: 5624 5624
Size: 6793
AhdI GACnn_n'nnGTC
Cuts at: 3316 5522 3316
Size: 2206 4587
AleI CACnn'nnGTG
Cuts at: 1166 3614 3908 1166
Size: 2448 294 4051
Fragments arranged by size:
4051 2448 294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3632 1184
Size: 2448 4345
AlwNI CAG_nnn'CTG
Cuts at: 2912 4536 6108 2912
Size: 1624 1572 3597
Fragments arranged by size:
3597 1624 1572
ApaI G_GGCC'C
Cuts at: 4054 4054
Size: 6793
ApaLI G'TGCA_C
Cuts at: 4850 6006 4850
Size: 1156 5637
ApoI r'AATT_y
Cuts at: 1493 3329 4102 4328 4480 6589 1493
Size: 1836 773 226 152 2109 1697
Fragments arranged by size:
2109 1836 1697 773 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3987 5347 160
Size: 872 2955 1360 1606
Fragments arranged by size:
2955 1606 1360 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
AvaI C'yCGr_G
Cuts at: 1870 1943 2443 3321 3394 3868 4118 1870
Size: 73 500 878 73 474 250 4545
Fragments arranged by size:
4545 878 500 474 250 73 73
BamHI G'GATC_C
Cuts at: 3426 4123 4490 3426
Size: 697 367 5729
Fragments arranged by size:
5729 697 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2821 4114 5569 618
Size: 325 217 627 1034 1293 1455 1842
Fragments arranged by size:
1842 1455 1293 1034 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4054 4112 1391
Size: 2663 58 4072
Fragments arranged by size:
4072 2663 58
BbeI G_GCGC'C
Cuts at: 2825 2825
Size: 6793
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5822
BbvCI CC'TCA_GC
Cuts at: 3452 3741 3452
Size: 289 6504
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2706 2740 4984 5018 1903
Size: 34 50 34 685 34 2244 34 3678
Fragments arranged by size:
3678 2244 685 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4685 5895 4685
Size: 1210 5583
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6793
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3033 5404 136
Size: 107 122 71 631 605 1361 2371 1525
Fragments arranged by size:
2371 1525 1361 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2085 3732 6788 2085
Size: 1647 3056 2090
Fragments arranged by size:
3056 2090 1647
BlpI GC'TnA_GC
Cuts at: 4210 4210
Size: 6793
Bme1580I G_kGCm'C
Cuts at: 1790 2538 3401 4054 4854 6010 1790
Size: 748 863 653 800 1156 2573
Fragments arranged by size:
2573 1156 863 800 748 653
BmgBI CAC'GTC
Cuts at: 1318 3472 1318
Size: 2154 4639
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2173 3804 4524
Size: 416 53 83 399 24 756 1631 720
Cuts at: 4524 5482 442
Size: 958 1753
Fragments arranged by size:
1753 1631 958 756 720 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2393 2492 2977 5453 1239
Size: 809 345 99 485 2476 2579
Fragments arranged by size:
2579 2476 809 485 345 99
Bpu10I CC'TnA_GC
Cuts at: 3007 3452 3741 3007
Size: 445 289 6059
Fragments arranged by size:
6059 445 289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3656 4918 5783 6081 3656
Size: 1262 865 298 4368
Fragments arranged by size:
4368 1262 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2834 3490 3800 5456 882
Size: 617 1335 656 310 1656 2219
Fragments arranged by size:
2219 1656 1335 656 617 310
BsaAI yAC'GTr
Cuts at: 493 1204 2122 4584 493
Size: 711 918 2462 2702
Fragments arranged by size:
2702 2462 918 711
BsaBI GATnn'nnATC
Cuts at: 2957 6787 2957
Size: 3830 2963
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2033 2822 3683 4600
Size: 53 83 186 936 500 789 861 917
Cuts at: 4600 4982 5617 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 917 861 789 635 500 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5226 5624 5898 6045 6456 5226
Size: 398 274 147 411 5563
Fragments arranged by size:
5563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2833 2863 3809 3839 1178
Size: 30 165 30 1430 30 946 30 4132
Fragments arranged by size:
4132 1430 946 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 3707 3961 1186
Size: 1417 1104 254 4018
Fragments arranged by size:
4018 1417 1104 254
BseYI C'CCAG_C
Cuts at: 3479 5996 6492 3479
Size: 2517 496 3780
Fragments arranged by size:
3780 2517 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2321 2635 3155 3491 3592 1163
Size: 573 585 314 520 336 101 4364
Fragments arranged by size:
4364 585 573 520 336 314 101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2716 4243 5004 6032 1540
Size: 275 186 715 1527 761 1028 2301
Fragments arranged by size:
2301 1527 1028 761 715 275 186
BsiHKAI G_wGCw'C
Cuts at: 2481 3605 4854 4939 6010 2481
Size: 1124 1249 85 1071 3264
Fragments arranged by size:
3264 1249 1124 1071 85
BsmI GAATG_Cn'
Cuts at: 2707 4304 4397 2707
Size: 1597 93 5103
Fragments arranged by size:
5103 1597 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2024 2040 3674 3949 2024
Size: 16 1634 275 4868
Fragments arranged by size:
4868 1634 275 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2481 2538 3401 3605 4054 4112 4854
Size: 399 691 57 863 204 449 58 742
Cuts at: 4854 4939 6010 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 863 742 691 449 399 204
85 58 57
BspEI T'CCGG_A
Cuts at: 6456 6456
Size: 6793
BspHI T'CATG_A
Cuts at: 4682 6412 4682
Size: 1730 5063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2521 3456 4148 844
Size: 1677 935 692 3489
Fragments arranged by size:
3489 1677 935 692
BsrBI CCG'CTC
Cuts at: 1394 1823 4680 1394
Size: 429 2857 3507
Fragments arranged by size:
3507 2857 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2104 5288 5462 65
Size: 1183 856 3184 174 1396
Fragments arranged by size:
3184 1396 1183 856 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3685 5437 5624 1700
Size: 110 1875 1752 187 2869
Fragments arranged by size:
2869 1875 1752 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6793
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6793
BssSI C'ACGA_G
Cuts at: 3920 4853 5865 3920
Size: 933 1012 4848
Fragments arranged by size:
4848 1012 933
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6793
BstBI TT'CG_AA
Cuts at: 2368 5597 2368
Size: 3229 3564
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6793
BstXI CCAn_nnnn'nTGG
Cuts at: 2946 2946
Size: 6793
BstYI r'GATC_y
Cuts at: 1972 2085 2622 2934 3162 3217 3426 3732 3756
Size: 113 537 312 228 55 209 306 24
Cuts at: 3756 4123 4490 4875 4892 6344 6430 6442 6788
Size: 367 367 385 17 1452 86 12 346
Cuts at: 6788 1972
Size: 1977
Fragments arranged by size:
1977 1452 537 385 367 367 346 312
306 228 209 113 86 55 24 17
12
Bsu36I CC'TnA_GG
Cuts at: 3278 3932 3278
Size: 654 6139
BtgI C'CryG_G
Cuts at: 513 1065 3583 3906 513
Size: 552 2518 323 3400
Fragments arranged by size:
3400 2518 552 323
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3220 487
Size: 683 56 1994 4060
Fragments arranged by size:
4060 1994 683 56
BtsI GCAGTG_nn'
Cuts at: 2163 3635 4309 5103 5123 2163
Size: 1472 674 794 20 3833
Fragments arranged by size:
3833 1472 794 674 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6793
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6758
DraI TTT'AAA
Cuts at: 2487 3069 4097 2487
Size: 582 1028 5183
Fragments arranged by size:
5183 1028 582
DraIII CAC_nnn'GTG
Cuts at: 3190 4060 3190
Size: 870 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5800 809
Size: 507 4484 1802
Fragments arranged by size:
4484 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2713 4240 5129
Size: 54 1425 50 275 18 883 1527 889
Cuts at: 5129 6758 8
Size: 1629 43
Fragments arranged by size:
1629 1527 1425 889 883 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2713 4240 1537
Size: 275 901 1527 4090
Fragments arranged by size:
4090 1527 901 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2832 4018 4723 1353
Size: 1479 1186 705 3423
Fragments arranged by size:
3423 1479 1186 705
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3818 3886 5380 5754 5900 387
Size: 1064 138 2229 68 1494 374 146 1280
Fragments arranged by size:
2229 1494 1280 1064 374 146 138 68
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2393 2492 2977 4856 5453 6240
Size: 393 416 345 99 485 1879 597 787
Cuts at: 6240 6508 1239
Size: 268 1524
Fragments arranged by size:
1879 1524 787 597 485 416 393 345
268 99
EcoNI CCTnn'n_nnAGG
Cuts at: 2630 2630
Size: 6793
EcoO109I rG'GnC_Cy
Cuts at: 2113 2448 3456 4050 2113
Size: 335 1008 594 4856
Fragments arranged by size:
4856 1008 594 335
EcoRI G'AATT_C
Cuts at: 4102 4480 4102
Size: 378 6415
FspI TGC'GCA
Cuts at: 5299 5299
Size: 6793
HaeII r_GCGC'y
Cuts at: 1678 2825 5940 1678
Size: 1147 3115 2531
Fragments arranged by size:
3115 2531 1147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2651 2683 2833 2865 3302
Size: 32 135 32 1079 32 150 32 437
Cuts at: 3302 3334 3724 3756 3953 3985 4058 4090 5434
Size: 32 390 32 197 32 73 32 1344
Cuts at: 5434 5466 5508 5540 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1344 1079 437 390 197 150 135
73 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3558 3771 4137 4383 669
Size: 988 281 1620 213 366 246 3079
Fragments arranged by size:
3079 1620 988 366 281 246 213
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6793
HpaI GTT'AAC
Cuts at: 3558 3771 4383 3558
Size: 213 612 5968
Fragments arranged by size:
5968 612 213
KasI G'GCGC_C
Cuts at: 2821 2821
Size: 6793
KpnI G_GTAC'C
Cuts at: 4118 4118
Size: 6793
MfeI C'AATT_G
Cuts at: 3612 4392 3612
Size: 780 6013
MluI A'CGCG_T
Cuts at: 6669 6669
Size: 6793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3195 3846 5907 6091 1305
Size: 1890 651 2061 184 2007
Fragments arranged by size:
2061 2007 1890 651 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4971
Fragments arranged by size:
4971 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3614 3857 3908 3920 4751 5110 5269
Size: 648 2448 243 51 12 831 359 159
Cuts at: 5269 518
Size: 2042
Fragments arranged by size:
2448 2042 831 648 359 243 159 51
12
MspA1I CmG'CkG
Cuts at: 1606 2051 2323 3042 3745 4209 4886 6034 6279
Size: 445 272 719 703 464 677 1148 245
Cuts at: 6279 1606
Size: 2120
Fragments arranged by size:
2120 1148 719 703 677 464 445 272
245
NarI GG'CG_CC
Cuts at: 2822 2822
Size: 6793
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6241
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6793
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6793
NotI GC'GGCC_GC
Cuts at: 4240 4240
Size: 6793
NspI r_CATG'y
Cuts at: 1132 1229 3919 4151 5696 1132
Size: 97 2690 232 1545 2229
Fragments arranged by size:
2690 2229 1545 232 97
PasI CC'CwG_GG
Cuts at: 1348 3029 3056 3594 1348
Size: 1681 27 538 4547
Fragments arranged by size:
4547 1681 538 27
PciI A'CATG_T
Cuts at: 5692 5692
Size: 6793
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6793
PfoI T'CCnGG_A
Cuts at: 2454 2851 2993 2454
Size: 397 142 6254
Fragments arranged by size:
6254 397 142
PmeI GTTT'AAAC
Cuts at: 4097 4097
Size: 6793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4862 4894 6375 6407 4862
Size: 32 1481 32 5248
Fragments arranged by size:
5248 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2113 3456 2113
Size: 1343 5450
PshAI GACnn'nnGTC
Cuts at: 1613 2784 1613
Size: 1171 5622
PsiI TTA'TAA
Cuts at: 2250 4363 2250
Size: 2113 4680
PspOMI G'GGCC_C
Cuts at: 4050 4050
Size: 6793
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3353 3385 3353
Size: 32 6761
PstI C_TGCA'G
Cuts at: 830 2157 2322 2748 3041 4145 830
Size: 1327 165 426 293 1104 3478
Fragments arranged by size:
3478 1327 1104 426 293 165
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6793
PvuII CAG'CTG
Cuts at: 2323 3042 3745 2323
Size: 719 703 5371
Fragments arranged by size:
5371 719 703
SalI G'TCGA_C
Cuts at: 1936 4135 1936
Size: 2199 4594
SbfI CC_TGCA'GG
Cuts at: 4145 4145
Size: 6793
ScaI AGT'ACT
Cuts at: 5041 6753 5041
Size: 1712 5081
SfcI C'TryA_G
Cuts at: 826 1045 2153 2318 2744 3037 3243 3995 4141
Size: 219 1108 165 426 293 206 752 146
Cuts at: 4141 5276 5957 6148 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1108 752 681 426 293 219
206 191 165 146
SfoI GGC'GCC
Cuts at: 2823 2823
Size: 6793
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
SgrAI Cr'CCGG_yG
Cuts at: 1700 3685 1700
Size: 1985 4808
SmaI CCC'GGG
Cuts at: 1872 2445 3323 3396 3870 4120 1872
Size: 573 878 73 474 250 4545
Fragments arranged by size:
4545 878 573 474 250 73
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3671 4897 5798 6060 820
Size: 197 926 1728 1226 901 262 1553
Fragments arranged by size:
1728 1553 1226 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4584 493
Size: 4091 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6793
SphI G_CATG'C
Cuts at: 1132 3919 4151 1132
Size: 2787 232 3774
Fragments arranged by size:
3774 2787 232
SspI AAT'ATT
Cuts at: 5 52 4717 5
Size: 47 4665 2081
Fragments arranged by size:
4665 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6793
StyI C'CwwG_G
Cuts at: 513 1065 2642 4232 513
Size: 552 1577 1590 3074
Fragments arranged by size:
3074 1590 1577 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2558 4820 4837 4990 5175 5620 325
Size: 1255 978 2262 17 153 185 445 1498
Fragments arranged by size:
2262 1498 1255 978 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3369 5039 6751
Size: 274 80 33 51 1443 1392 1670 1712
Cuts at: 6751 96
Size: 138
Fragments arranged by size:
1712 1670 1443 1392 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3266 3298 3809 3841 6753 6785 3266
Size: 32 511 32 2912 32 3274
Fragments arranged by size:
3274 2912 511 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6793
XbaI T'CTAG_A
Cuts at: 4129 4129
Size: 6793
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3859 3859
Size: 6793
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6793
XmaI C'CCGG_G
Cuts at: 1870 2443 3321 3394 3868 4118 1870
Size: 573 878 73 474 250 4545
Fragments arranged by size:
4545 878 573 474 250 73
XmnI GAAnn'nnTTC
Cuts at: 2295 2691 4922 2295
Size: 396 2231 4166
Fragments arranged by size:
4166 2231 396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2034 4601 276
Size: 53 83 186 936 500 2567 2468
Fragments arranged by size:
2567 2468 936 500 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BstZ17I EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI