Restriction map for
pFN21AA1909
(Circular) (Six-base) MAPSORT of: pFN21AA1909.seq Check: 594 from: 1 to: 8518
pFN21AA1909 8518 bp
With 182 enzymes: SgfI *
October 20, 2009 10:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2560 3695 4393 2560
Size: 1135 698 6685
Fragments arranged by size:
6685 1135 698
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2583 5133 6328 278
Size: 53 83 186 936 1047 2550 1195 2468
Fragments arranged by size:
2550 2468 1195 1047 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5133 5861 1937
Size: 3196 728 4594
Fragments arranged by size:
4594 3196 728
Acc65I G'GTAC_C
Cuts at: 5839 5839
Size: 8518
AclI AA'CG_TT
Cuts at: 1563 6645 7018 1563
Size: 5082 373 3063
Fragments arranged by size:
5082 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2969 3740 4240 6581 7965 8233 1632
Size: 1337 771 500 2341 1384 268 1917
Fragments arranged by size:
2341 1917 1384 1337 771 500 268
AfeI AGC'GCT
Cuts at: 3984 4619 3984
Size: 635 7883
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8321
AflIII A'CryG_T
Cuts at: 1203 7417 8394 1203
Size: 6214 977 1327
Fragments arranged by size:
6214 1327 977
AgeI A'CCGG_T
Cuts at: 7349 7349
Size: 8518
AhdI GACnn_n'nnGTC
Cuts at: 2172 4045 7247 2172
Size: 1873 3202 3443
Fragments arranged by size:
3443 3202 1873
AleI CACnn'nnGTG
Cuts at: 1166 5525 1166
Size: 4359 4159
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3888 4734 4970 1184
Size: 2704 846 236 4732
Fragments arranged by size:
4732 2704 846 236
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5331 5363 5331
Size: 32 8486
AlwNI CAG_nnn'CTG
Cuts at: 2219 2575 2593 3049 3376 4135 4852 4894 5694
Size: 356 18 456 327 759 717 42 800
Cuts at: 5694 5715 6261 7833 2219
Size: 21 546 1572 2904
Fragments arranged by size:
2904 1572 800 759 717 546 456 356
327 42 21 18
ApaI G_GGCC'C
Cuts at: 2287 3606 4019 2287
Size: 1319 413 6786
Fragments arranged by size:
6786 1319 413
ApaLI G'TGCA_C
Cuts at: 2957 3335 6575 7731 2957
Size: 378 3240 1156 3744
Fragments arranged by size:
3744 3240 1156 378
ApoI r'AATT_y
Cuts at: 1493 2963 3097 4990 5827 6053 6205 8314 1493
Size: 1470 134 1893 837 226 152 2109 1697
Fragments arranged by size:
2109 1893 1697 1470 837 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 7072 160
Size: 872 6040 1606
Fragments arranged by size:
6040 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8518
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2148 2513 2628 3698 4366 5665
Size: 73 111 94 365 115 1070 668 1299
Cuts at: 5665 5843 1870
Size: 178 4545
Fragments arranged by size:
4545 1299 1070 668 365 178 115 111
94 73
AvrII C'CTAG_G
Cuts at: 2344 2344
Size: 8518
BamHI G'GATC_C
Cuts at: 5307 5848 6215 5307
Size: 541 367 7610
Fragments arranged by size:
7610 541 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2263 2560 3233 3894 3938
Size: 325 217 627 476 297 673 661 44
Cuts at: 3938 5756 5839 7294 618
Size: 1818 83 1455 1842
Fragments arranged by size:
1842 1818 1455 673 661 627 476 325
297 217 83 44
BanII G_rGCy'C
Cuts at: 1391 2287 2945 3606 3857 4011 4019 4125 4331
Size: 896 658 661 251 154 8 106 206
Cuts at: 4331 4374 4905 5446 5681 5785 5837 1391
Size: 43 531 541 235 104 52 4072
Fragments arranged by size:
4072 896 661 658 541 531 251 235
206 154 106 104 52 43 8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2418 3204 3282 4246 5088 928
Size: 971 519 786 78 964 842 4358
Fragments arranged by size:
4358 971 964 842 786 519 78
BbvCI CC'TCA_GC
Cuts at: 2428 3251 4203 4383 2428
Size: 823 952 180 6563
Fragments arranged by size:
6563 952 823 180
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2477 2511 6709 6743 1903
Size: 34 540 34 4198 34 3678
Fragments arranged by size:
4198 3678 540 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4472 6410 7620 4472
Size: 1938 1210 5370
Fragments arranged by size:
5370 1938 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8518
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2500 3881 4085
Size: 107 122 71 631 605 828 1381 204
Cuts at: 4085 7129 136
Size: 3044 1525
Fragments arranged by size:
3044 1525 1381 828 631 605 204 122
107 71
BglII A'GATC_T
Cuts at: 2243 8513 2243
Size: 6270 2248
BlpI GC'TnA_GC
Cuts at: 2706 4727 5798 5935 2706
Size: 2021 1071 137 5289
Fragments arranged by size:
5289 2021 1071 137
Bme1580I G_kGCm'C
Cuts at: 1790 2266 2287 2565 2961 3236 3339 3606 3877
Size: 476 21 278 396 275 103 267 271
Cuts at: 3877 4019 5498 5759 6579 7735 1790
Size: 142 1479 261 820 1156 2573
Fragments arranged by size:
2573 1479 1156 820 476 396 278 275
271 267 261 142 103 21
BmgBI CAC'GTC
Cuts at: 1318 4987 1318
Size: 3669 4849
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2115 2375 6249
Size: 416 53 83 399 24 698 260 3874
Cuts at: 6249 7207 442
Size: 958 1753
Fragments arranged by size:
3874 1753 958 698 416 399 260 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8518
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2155 3691 4120 2155
Size: 1536 429 6553
Fragments arranged by size:
6553 1536 429
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2103 2260 2311 3245 3314 3353 3398 4115
Size: 864 157 51 934 69 39 45 717
Cuts at: 4115 4289 4634 4829 5635 7178 1239
Size: 174 345 195 806 1543 2579
Fragments arranged by size:
2579 1543 934 864 806 717 345 195
174 157 69 51 45 39
Bpu10I CC'TnA_GC
Cuts at: 2428 3251 3506 4203 4383 4474 2428
Size: 823 255 697 180 91 6472
Fragments arranged by size:
6472 823 697 255 180 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4841 5221 6643 7508 7806 4841
Size: 380 1422 865 298 5553
Fragments arranged by size:
5553 1422 865 380 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2148 2625 5400 7181 882
Size: 617 649 477 2775 1781 2219
Fragments arranged by size:
2775 2219 1781 649 617 477
BsaAI yAC'GTr
Cuts at: 493 1204 4687 6309 493
Size: 711 3483 1622 2702
Fragments arranged by size:
3483 2702 1622 711
BsaBI GATnn'nnATC
Cuts at: 8512 8512
Size: 8518
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2580 3398 4968 5130
Size: 53 83 186 936 1047 818 1570 162
Cuts at: 5130 6325 6707 7342 275
Size: 1195 382 635 1451
Fragments arranged by size:
1570 1451 1195 1047 936 818 635 382
186 162 83 53
BsaWI w'CCGG_w
Cuts at: 5409 6951 7349 7623 7770 8181 5409
Size: 1542 398 274 147 411 5746
Fragments arranged by size:
5746 1542 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3383 3413 4035 4065 4778
Size: 30 165 30 1980 30 622 30 713
Cuts at: 4778 4808 1178
Size: 30 4888
Fragments arranged by size:
4888 1980 713 622 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 3268 3344 3619 3667 3904 4043 4400
Size: 874 1208 76 275 48 237 139 357
Cuts at: 4400 5524 5584 5605 1186
Size: 1124 60 21 4099
Fragments arranged by size:
4099 1208 1124 874 357 275 237 139
76 60 48 21
BseYI C'CCAG_C
Cuts at: 2198 2474 2552 3830 3917 4894 5632 5656 7721
Size: 276 78 1278 87 977 738 24 2065
Cuts at: 7721 8217 2198
Size: 496 2499
Fragments arranged by size:
2499 2065 1278 977 738 496 276 87
78 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2383 2675 3553 4835 5445 5475 1163
Size: 573 647 292 878 1282 610 30 4206
Fragments arranged by size:
4206 1282 878 647 610 573 292 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3319 5163 5470 5968 6729 7757
Size: 275 186 1318 1844 307 498 761 1028
Cuts at: 7757 1540
Size: 2301
Fragments arranged by size:
2301 1844 1318 1028 761 498 307 275
186
BsiHKAI G_wGCw'C
Cuts at: 2945 2961 3339 3384 4033 4905 6579 6664 7735
Size: 16 378 45 649 872 1674 85 1071
Cuts at: 7735 2945
Size: 3728
Fragments arranged by size:
3728 1674 1071 872 649 378 85 45
16
BsmI GAATG_Cn'
Cuts at: 6029 6122 6029
Size: 93 8425
BsmBI CGTCTCn'nnnn_
Cuts at: 2821 3167 3371 3575 5744 2821
Size: 346 204 204 2169 5595
Fragments arranged by size:
5595 2169 346 204 204
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2266 2287 2565 2945 2961 3236 3339
Size: 399 476 21 278 380 16 275 103
Cuts at: 3339 3384 3606 3857 3877 4011 4019 4033 4125
Size: 45 222 251 20 134 8 14 92
Cuts at: 4125 4331 4374 4905 5446 5498 5681 5759 5785
Size: 206 43 531 541 52 183 78 26
Cuts at: 5785 5837 6579 6664 7735 1391
Size: 52 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 541 531 476 399 380
278 275 251 222 206 183 134 103
92 85 78 52 52 45 43 26
21 20 16 14 8
BspEI T'CCGG_A
Cuts at: 8181 8181
Size: 8518
BspHI T'CATG_A
Cuts at: 6407 8137 6407
Size: 1730 6788
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2560 2647 3695 3958 4393 4809 4860 4962
Size: 1716 87 1048 263 435 416 51 102
Cuts at: 4962 5873 844
Size: 911 3489
Fragments arranged by size:
3489 1716 1048 911 435 416 263 102
87 51
BsrBI CCG'CTC
Cuts at: 1394 1823 3564 6405 1394
Size: 429 1741 2841 3507
Fragments arranged by size:
3507 2841 1741 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2461 4954 7013 7187 65
Size: 1183 1213 2493 2059 174 1396
Fragments arranged by size:
2493 2059 1396 1213 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7162 7349 1700
Size: 110 5352 187 2869
Fragments arranged by size:
5352 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 5139 96
Size: 5043 3475
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8518
BssSI C'ACGA_G
Cuts at: 6578 7590 6578
Size: 1012 7506
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8518
BstBI TT'CG_AA
Cuts at: 7322 7322
Size: 8518
BstEII G'GTnAC_C
Cuts at: 1184 4273 1184
Size: 3089 5429
BstXI CCAn_nnnn'nTGG
Cuts at: 2032 2032
Size: 8518
BstYI r'GATC_y
Cuts at: 1972 2243 3030 5307 5848 6215 6600 6617 8069
Size: 271 787 2277 541 367 385 17 1452
Cuts at: 8069 8155 8167 8513 1972
Size: 86 12 346 1977
Fragments arranged by size:
2277 1977 1452 787 541 385 367 346
271 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4055 4055
Size: 8518
BtgI C'CryG_G
Cuts at: 513 1065 2004 3019 3513 3636 3743 4083 4641
Size: 552 939 1015 494 123 107 340 558
Cuts at: 4641 4735 4962 4975 5364 513
Size: 94 227 13 389 3667
Fragments arranged by size:
3667 1015 939 558 552 494 389 340
227 123 107 94 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7779
Fragments arranged by size:
7779 683 56
BtsI GCAGTG_nn'
Cuts at: 2655 3203 3720 4627 5433 5463 5775 6034 6828
Size: 548 517 907 806 30 312 259 794
Cuts at: 6828 6848 2655
Size: 20 4325
Fragments arranged by size:
4325 907 806 794 548 517 312 259
30 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8518
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2507 2542 3746 3781 529
Size: 35 1943 35 1204 35 5266
Fragments arranged by size:
5266 1943 1204 35 35 35
DraI TTT'AAA
Cuts at: 5822 5822
Size: 8518
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3565 5486 7525 809
Size: 507 2249 1921 2039 1802
Fragments arranged by size:
2249 2039 1921 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2528 3549 4137
Size: 54 1425 50 275 18 698 1021 588
Cuts at: 4137 4434 4638 4824 4959 5160 5965 6854 8483
Size: 297 204 186 135 201 805 889 1629
Cuts at: 8483 8
Size: 43
Fragments arranged by size:
1629 1425 1021 889 805 698 588 297
275 204 201 186 135 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5160 5965 1537
Size: 275 3348 805 4090
Fragments arranged by size:
4090 3348 805 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2238 3255 3795 4662 5602 6448 1353
Size: 885 1017 540 867 940 846 3423
Fragments arranged by size:
3423 1017 940 885 867 846 540
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2242 7105 7479 7625 387
Size: 1064 138 653 4863 374 146 1280
Fragments arranged by size:
4863 1280 1064 653 374 146 138
EcoICRI GAG'CTC
Cuts at: 2943 4903 2943
Size: 1960 6558
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 2260 2311 2969 3245 3314 3353
Size: 393 471 157 51 658 276 69 39
Cuts at: 3353 3398 3740 4115 4240 4289 4634 4829 5635
Size: 45 342 375 125 49 345 195 806
Cuts at: 5635 6581 7178 7965 8233 1239
Size: 946 597 787 268 1524
Fragments arranged by size:
1524 946 806 787 658 597 471 393
375 345 342 276 268 195 157 125
69 51 49 45 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2257 3812 4856 4880 2257
Size: 1555 1044 24 5895
Fragments arranged by size:
5895 1555 1044 24
EcoO109I rG'GnC_Cy
Cuts at: 2050 2059 2095 2124 2283 2364 2390 2625 2761
Size: 9 36 29 159 81 26 235 136
Cuts at: 2761 2824 3502 3602 3603 3805 4039 4060 4264
Size: 63 678 100 1 202 234 21 204
Cuts at: 4264 4531 4849 5473 2050
Size: 267 318 624 5095
Fragments arranged by size:
5095 678 624 318 267 235 234 204
202 159 136 100 81 63 36 29
26 21 9 1
EcoRI G'AATT_C
Cuts at: 3097 5827 6205 3097
Size: 2730 378 5410
Fragments arranged by size:
5410 2730 378
EcoRV GAT'ATC
Cuts at: 5707 5707
Size: 8518
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2435 3824 5167 2435
Size: 1389 1343 5786
Fragments arranged by size:
5786 1389 1343
FspI TGC'GCA
Cuts at: 7024 7024
Size: 8518
HaeII r_GCGC'y
Cuts at: 1678 3513 3986 4621 7665 1678
Size: 1835 473 635 3044 2531
Fragments arranged by size:
3044 2531 1835 635 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2158 2190 2876 2908 3383
Size: 32 135 32 586 32 686 32 475
Cuts at: 3383 3410 3415 3442 3643 3675 4031 4063 4336
Size: 27 5 27 201 32 356 32 273
Cuts at: 4336 4368 4778 4810 5703 5735 7159 7191 7233
Size: 32 410 32 893 32 1424 32 42
Cuts at: 7233 7265 1373
Size: 32 2626
Fragments arranged by size:
2626 1424 893 686 586 475 410 356
273 201 135 42 32 32 32 32
32 32 32 32 32 32 32 27
27 5
HincII GTy'rAC
Cuts at: 669 1657 1938 2971 5862 6108 669
Size: 988 281 1033 2891 246 3079
Fragments arranged by size:
3079 2891 1033 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3185 748
Size: 2437 6081
HpaI GTT'AAC
Cuts at: 6108 6108
Size: 8518
KpnI G_GTAC'C
Cuts at: 5843 5843
Size: 8518
MfeI C'AATT_G
Cuts at: 6117 6117
Size: 8518
MluI A'CGCG_T
Cuts at: 8394 8394
Size: 8518
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4695 7632 7816 1305
Size: 3390 2937 184 2007
Fragments arranged by size:
3390 2937 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2530 3551 4139 4826 4961
Size: 54 1425 343 698 1021 588 687 135
Cuts at: 4961 10
Size: 3567
Fragments arranged by size:
3567 1425 1021 698 687 588 343 135
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3870 4248 4947 5525 6476 6835 6994
Size: 648 2704 378 699 578 951 359 159
Cuts at: 6994 518
Size: 2042
Fragments arranged by size:
2704 2042 951 699 648 578 378 359
159
MspA1I CmG'CkG
Cuts at: 1606 2478 2804 2976 2983 3376 3448 3594 4309
Size: 872 326 172 7 393 72 146 715
Cuts at: 4309 4765 4869 4936 4977 5011 5525 5934 6611
Size: 456 104 67 41 34 514 409 677
Cuts at: 6611 7759 8004 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 872 715 677 514 456 409
393 326 245 172 146 104 72 67
41 34 7
NcoI C'CATG_G
Cuts at: 513 1065 2004 3019 4735 4962 5364 513
Size: 552 939 1015 1716 227 402 3667
Fragments arranged by size:
3667 1716 1015 939 552 402 227
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8518
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8518
NotI GC'GGCC_GC
Cuts at: 5965 5965
Size: 8518
NspI r_CATG'y
Cuts at: 1132 1229 4322 5069 5876 7421 1132
Size: 97 3093 747 807 1545 2229
Fragments arranged by size:
3093 2229 1545 807 747 97
PasI CC'CwG_GG
Cuts at: 1348 2032 2621 3733 4012 4019 4221 4528 4882
Size: 684 589 1112 279 7 202 307 354
Cuts at: 4882 1348
Size: 4984
Fragments arranged by size:
4984 1112 684 589 354 307 279 202
7
PciI A'CATG_T
Cuts at: 7417 7417
Size: 8518
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3376 4135 5113 5356 1108
Size: 2268 759 978 243 4270
Fragments arranged by size:
4270 2268 978 759 243
PfoI T'CCnGG_A
Cuts at: 2212 2757 3192 3387 3405 3486 4605 4845 2212
Size: 545 435 195 18 81 1119 240 5885
Fragments arranged by size:
5885 1119 545 435 240 195 81 18
PmeI GTTT'AAAC
Cuts at: 5822 5822
Size: 8518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6587 6619 8100 8132 6587
Size: 32 1481 32 6973
Fragments arranged by size:
6973 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2095 2124 2364 2390 2625 2761 4039 4849
Size: 36 29 240 26 235 136 1278 810
Cuts at: 4849 5473 2059
Size: 624 5104
Fragments arranged by size:
5104 1278 810 624 240 235 136 36
29 26
PshAI GACnn'nnGTC
Cuts at: 1613 2583 5294 1613
Size: 970 2711 4837
Fragments arranged by size:
4837 2711 970
PsiI TTA'TAA
Cuts at: 6088 6088
Size: 8518
PspOMI G'GGCC_C
Cuts at: 2283 3602 4015 2283
Size: 1319 413 6786
Fragments arranged by size:
6786 1319 413
PstI C_TGCA'G
Cuts at: 830 2072 2224 2296 2601 3688 3774 5770 5870
Size: 1242 152 72 305 1087 86 1996 100
Cuts at: 5870 830
Size: 3478
Fragments arranged by size:
3478 1996 1242 1087 305 152 100 86
72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8518
PvuII CAG'CTG
Cuts at: 2804 2976 2983 3376 3448 3594 4869 4936 5011
Size: 172 7 393 72 146 1275 67 75
Cuts at: 5011 2804
Size: 6311
Fragments arranged by size:
6311 1275 393 172 146 75 72 67
7
SacI G_AGCT'C
Cuts at: 2945 4905 2945
Size: 1960 6558
SacII CC_GC'GG
Cuts at: 4978 4978
Size: 8518
SalI G'TCGA_C
Cuts at: 1936 5860 1936
Size: 3924 4594
SanDI GG'GwC_CC
Cuts at: 2124 2364 2390 2625 4039 5473 2124
Size: 240 26 235 1414 1434 5169
Fragments arranged by size:
5169 1434 1414 240 235 26
SapI GCTCTTCn'nnn_
Cuts at: 3255 4662 3255
Size: 1407 7111
SbfI CC_TGCA'GG
Cuts at: 5870 5870
Size: 8518
ScaI AGT'ACT
Cuts at: 6766 8478 6766
Size: 1712 6806
SfcI C'TryA_G
Cuts at: 826 1045 2068 2220 2292 2597 3013 3092 3443
Size: 219 1023 152 72 305 416 79 351
Cuts at: 3443 3684 3770 4284 4693 5766 5866 7001 7682
Size: 241 86 514 409 1073 100 1135 681
Cuts at: 7682 7873 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1073 1023 681 514 416 409
351 305 241 219 191 152 100 86
79 72
SfiI GGCCn_nnn'nGGCC
Cuts at: 4085 4085
Size: 8518
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8518
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8518
SmaI CCC'GGG
Cuts at: 1872 2150 3700 5667 5845 1872
Size: 278 1550 1967 178 4545
Fragments arranged by size:
4545 1967 1550 278 178
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4856 5200 6622 7523 7785 820
Size: 197 926 2913 344 1422 901 262 1553
Fragments arranged by size:
2913 1553 1422 926 901 344 262 197
SnaBI TAC'GTA
Cuts at: 493 6309 493
Size: 5816 2702
SpeI A'CTAG_T
Cuts at: 152 4247 152
Size: 4095 4423
SphI G_CATG'C
Cuts at: 1132 4322 5876 1132
Size: 3190 1554 3774
Fragments arranged by size:
3774 3190 1554
SspI AAT'ATT
Cuts at: 5 52 6442 5
Size: 47 6390 2081
Fragments arranged by size:
6390 2081 47
StuI AGG'CCT
Cuts at: 1517 2332 2905 3692 1517
Size: 815 573 787 6343
Fragments arranged by size:
6343 815 787 573
StyI C'CwwG_G
Cuts at: 513 1065 2004 2344 3019 4635 4735 4962 5364
Size: 552 939 340 675 1616 100 227 402
Cuts at: 5364 5957 513
Size: 593 3074
Fragments arranged by size:
3074 1616 939 675 593 552 402 340
227 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2212 2238 2281 3134 3333 4142 5306
Size: 1255 632 26 43 853 199 809 1164
Cuts at: 5306 5774 6545 6562 6715 6900 7345 325
Size: 468 771 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1164 853 809 771 632 468
445 199 185 153 43 26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2727 4461 5139
Size: 274 80 33 51 1443 750 1734 678
Cuts at: 5139 6764 8476 96
Size: 1625 1712 138
Fragments arranged by size:
1734 1712 1625 1443 750 678 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2375 3897 3929 4035 4067 8478 8510 2343
Size: 32 1522 32 106 32 4411 32 2351
Fragments arranged by size:
4411 2351 1522 106 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 5471 1316
Size: 4155 4363
XbaI T'CTAG_A
Cuts at: 2176 5854 2176
Size: 3678 4840
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2095 2572 4090 4132 4873 2095
Size: 477 1518 42 741 5740
Fragments arranged by size:
5740 1518 741 477 42
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8518
XmaI C'CCGG_G
Cuts at: 1870 2148 3698 5665 5843 1870
Size: 278 1550 1967 178 4545
Fragments arranged by size:
4545 1967 1550 278 178
XmnI GAAnn'nnTTC
Cuts at: 2415 3101 6647 2415
Size: 686 3546 4286
Fragments arranged by size:
4286 3546 686
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2581 5131 6326 276
Size: 53 83 186 936 1047 2550 1195 2468
Fragments arranged by size:
2550 2468 1195 1047 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SexAI SfoI SrfI SwaI