Restriction map for
pFN21AA1885
(Circular) (Six-base) MAPSORT of: pFN21AA1885.seq Check: 4301 from: 1 to: 7894
pFN21AA1885 7894 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2218 4030 4726 2218
Size: 1812 696 5386
Fragments arranged by size:
5386 1812 696
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4464 5704 278
Size: 53 83 186 936 2928 1240 2468
Fragments arranged by size:
2928 2468 1240 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4464 4950 5237 1937
Size: 2527 486 287 4594
Fragments arranged by size:
4594 2527 486 287
Acc65I G'GTAC_C
Cuts at: 2343 5215 2343
Size: 2872 5022
AclI AA'CG_TT
Cuts at: 1563 6021 6394 1563
Size: 4458 373 3063
Fragments arranged by size:
4458 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2799 3452 3913 4608 5957 7341 7609 1632
Size: 1167 653 461 695 1349 1384 268 1917
Fragments arranged by size:
1917 1384 1349 1167 695 653 461 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7697
AflIII A'CryG_T
Cuts at: 1203 3157 6793 7770 1203
Size: 1954 3636 977 1327
Fragments arranged by size:
3636 1954 1327 977
AgeI A'CCGG_T
Cuts at: 3306 6725 3306
Size: 3419 4475
AhdI GACnn_n'nnGTC
Cuts at: 6623 6623
Size: 7894
AleI CACnn'nnGTG
Cuts at: 1166 2936 1166
Size: 1770 6124
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7894
AlwNI CAG_nnn'CTG
Cuts at: 2771 3674 4045 4214 4528 4606 4668 4811 4969
Size: 903 371 169 314 78 62 143 158
Cuts at: 4969 5155 5637 7209 2771
Size: 186 482 1572 3456
Fragments arranged by size:
3456 1572 903 482 371 314 186 169
158 143 78 62
ApaI G_GGCC'C
Cuts at: 2301 3223 3472 3502 4518 4921 2301
Size: 922 249 30 1016 403 5274
Fragments arranged by size:
5274 1016 922 403 249 30
ApaLI G'TGCA_C
Cuts at: 5951 7107 5951
Size: 1156 6738
ApoI r'AATT_y
Cuts at: 1493 4069 4292 5203 5429 5581 7690 1493
Size: 2576 223 911 226 152 2109 1697
Fragments arranged by size:
2576 2109 1697 911 226 223 152
AseI AT'TA_AT
Cuts at: 160 1032 6448 160
Size: 872 5416 1606
Fragments arranged by size:
5416 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7894
AvaI C'yCGr_G
Cuts at: 1870 1943 3318 3471 4401 4716 4785 5219 1870
Size: 73 1375 153 930 315 69 434 4545
Fragments arranged by size:
4545 1375 930 434 315 153 73 69
BamHI G'GATC_C
Cuts at: 3739 4131 4347 5224 5591 3739
Size: 392 216 877 367 6042
Fragments arranged by size:
6042 877 392 367 216
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2343 2511 2714 4686 4853
Size: 325 217 627 556 168 203 1972 167
Cuts at: 4853 5215 6670 618
Size: 362 1455 1842
Fragments arranged by size:
1972 1842 1455 627 556 362 325 217
203 168 167
BanII G_rGCy'C
Cuts at: 1391 2106 2301 2710 3223 3239 3472 3502 3510
Size: 715 195 409 513 16 233 30 8
Cuts at: 3510 4410 4518 4596 4750 4805 4848 4921 5186
Size: 900 108 78 154 55 43 73 265
Cuts at: 5186 5193 5213 1391
Size: 7 20 4072
Fragments arranged by size:
4072 900 715 513 409 265 233 195
154 108 78 73 55 43 30 20
16 8 7
BbeI G_GCGC'C
Cuts at: 4690 4690
Size: 7894
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3293 928
Size: 971 1394 5529
Fragments arranged by size:
5529 1394 971
BbvCI CC'TCA_GC
Cuts at: 2051 3675 4311 2051
Size: 1624 636 5634
Fragments arranged by size:
5634 1624 636
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4221 4255 6085 6119 1903
Size: 34 50 34 2200 34 1830 34 3678
Fragments arranged by size:
3678 2200 1830 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2441 5786 6996 2441
Size: 3345 1210 3339
Fragments arranged by size:
3345 3339 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7894
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4097 4615 6505
Size: 107 122 71 631 605 2425 518 1890
Cuts at: 6505 136
Size: 1525
Fragments arranged by size:
2425 1890 1525 631 605 518 122 107
71
BglII A'GATC_T
Cuts at: 2688 4248 7889 2688
Size: 1560 3641 2693
Fragments arranged by size:
3641 2693 1560
BlpI GC'TnA_GC
Cuts at: 2630 5311 2630
Size: 2681 5213
Bme1580I G_kGCm'C
Cuts at: 1790 2301 3167 3223 3472 3502 4518 4856 4921
Size: 511 866 56 249 30 1016 338 65
Cuts at: 4921 5955 7111 1790
Size: 1034 1156 2573
Fragments arranged by size:
2573 1156 1034 1016 866 511 338 249
65 56 30
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7894
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2737 5625 6583
Size: 416 53 83 399 24 1320 2888 958
Cuts at: 6583 442
Size: 1753
Fragments arranged by size:
2888 1753 1320 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2198 2251 3054 3171 3353 3524 3752 4490
Size: 959 53 803 117 182 171 228 738
Cuts at: 4490 4721 5090 5132 5154 6554 1239
Size: 231 369 42 22 1400 2579
Fragments arranged by size:
2579 1400 959 803 738 369 231 228
182 171 117 53 42 22
Bpu10I CC'TnA_GC
Cuts at: 2051 3675 4311 4352 4742 2051
Size: 1624 636 41 390 5203
Fragments arranged by size:
5203 1624 636 390 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3253 4929 6019 6884 7182 3253
Size: 1676 1090 865 298 3965
Fragments arranged by size:
3965 1676 1090 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2408 3803 6557 882
Size: 617 909 1395 2754 2219
Fragments arranged by size:
2754 2219 1395 909 617
BsaAI yAC'GTr
Cuts at: 493 1204 4761 5685 493
Size: 711 3557 924 2702
Fragments arranged by size:
3557 2702 924 711
BsaBI GATnn'nnATC
Cuts at: 7888 7888
Size: 7894
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4461 4687 5701 6083
Size: 53 83 186 936 2928 226 1014 382
Cuts at: 6083 6718 275
Size: 635 1451
Fragments arranged by size:
2928 1451 1014 936 635 382 226 186
83 53
BsaWI w'CCGG_w
Cuts at: 2514 2817 3306 6327 6725 6999 7146 7557 2514
Size: 303 489 3021 398 274 147 411 2851
Fragments arranged by size:
3021 2851 489 411 398 303 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2052 2082 2119 2149 2189
Size: 30 165 30 649 30 37 30 40
Cuts at: 2189 2219 1178
Size: 30 6853
Fragments arranged by size:
6853 649 165 40 37 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2632 2752 4318 4328 1186
Size: 1446 120 1566 10 4752
Fragments arranged by size:
4752 1566 1446 120 10
BseYI C'CCAG_C
Cuts at: 3906 4637 4661 4914 7097 7593 3906
Size: 731 24 253 2183 496 4207
Fragments arranged by size:
4207 2183 731 496 253 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2444 4415 4754 1163
Size: 573 708 1971 339 4303
Fragments arranged by size:
4303 1971 708 573 339
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5344 6105 7133 1540
Size: 275 186 3343 761 1028 2301
Fragments arranged by size:
3343 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2710 3510 4410 4596 4683 4805 5193 5955 6040
Size: 800 900 186 87 122 388 762 85
Cuts at: 6040 7111 2710
Size: 1071 3493
Fragments arranged by size:
3493 1071 900 800 762 388 186 122
87 85
BsmI GAATG_Cn'
Cuts at: 4261 5405 5498 4261
Size: 1144 93 6657
Fragments arranged by size:
6657 1144 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3017 3017
Size: 7894
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2106 2301 2710 3167 3223 3239 3472
Size: 399 316 195 409 457 56 16 233
Cuts at: 3472 3502 3510 4410 4518 4596 4683 4750 4805
Size: 30 8 900 108 78 87 67 55
Cuts at: 4805 4848 4856 4921 5186 5193 5213 5955 6040
Size: 43 8 65 265 7 20 742 85
Cuts at: 6040 7111 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 900 742 457 409 399 316
265 233 195 108 87 85 78 67
65 56 55 43 30 20 16 8
8 7
BspEI T'CCGG_A
Cuts at: 7557 7557
Size: 7894
BspHI T'CATG_A
Cuts at: 5783 7513 5783
Size: 1730 6164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2218 3435 4030 4726 5249 844
Size: 1374 1217 595 696 523 3489
Fragments arranged by size:
3489 1374 1217 696 595 523
BsrBI CCG'CTC
Cuts at: 1394 1823 4387 4705 5781 1394
Size: 429 2564 318 1076 3507
Fragments arranged by size:
3507 2564 1076 429 318
BsrDI GCAATG_nn'
Cuts at: 65 1248 3249 6389 6563 65
Size: 1183 2001 3140 174 1396
Fragments arranged by size:
3140 2001 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2886 3306 6538 6725 1700
Size: 110 1076 420 3232 187 2869
Fragments arranged by size:
3232 2869 1076 420 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7894
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7894
BssSI C'ACGA_G
Cuts at: 2501 5954 6966 2501
Size: 3453 1012 3429
Fragments arranged by size:
3453 3429 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2518 2567 4045 4606 794
Size: 1724 49 1478 561 4082
Fragments arranged by size:
4082 1724 1478 561 49
BstBI TT'CG_AA
Cuts at: 6698 6698
Size: 7894
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7894
BstXI CCAn_nnnn'nTGG
Cuts at: 2043 3487 2043
Size: 1444 6450
BstYI r'GATC_y
Cuts at: 1972 2111 2688 3739 4131 4248 4347 4983 5224
Size: 139 577 1051 392 117 99 636 241
Cuts at: 5224 5591 5976 5993 7445 7531 7543 7889 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1051 636 577 392 385 367
346 241 139 117 99 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2238 2648 4521 2238
Size: 410 1873 5611
Fragments arranged by size:
5611 1873 410
BtgI C'CryG_G
Cuts at: 513 1065 2107 2348 2748 2973 3381 3951 4177
Size: 552 1042 241 400 225 408 570 226
Cuts at: 4177 513
Size: 4230
Fragments arranged by size:
4230 1042 570 552 408 400 241 226
225
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7155
Fragments arranged by size:
7155 683 56
BtsI GCAGTG_nn'
Cuts at: 2669 3180 5410 6204 6224 2669
Size: 511 2230 794 20 4339
Fragments arranged by size:
4339 2230 794 511 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7894
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2519 2554 529
Size: 35 1955 35 5869
Fragments arranged by size:
5869 1955 35 35
DraI TTT'AAA
Cuts at: 5198 5198
Size: 7894
DraIII CAC_nnn'GTG
Cuts at: 4934 4934
Size: 7894
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3099 3569 6901 809
Size: 507 1783 470 3332 1802
Fragments arranged by size:
3332 1802 1783 507 470
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2467 2484 2899
Size: 54 1425 50 275 18 637 17 415
Cuts at: 2899 3048 4089 5341 6230 7859 8
Size: 149 1041 1252 889 1629 43
Fragments arranged by size:
1629 1425 1252 1041 889 637 415 275
149 54 50 43 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 5341 1537
Size: 275 3529 4090
Fragments arranged by size:
4090 3529 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3129 3636 4266 4584 4832 5824 1353
Size: 1776 507 630 318 248 992 3423
Fragments arranged by size:
3423 1776 992 630 507 318 248
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4544 6481 6855 7001 387
Size: 1064 138 2955 1937 374 146 1280
Fragments arranged by size:
2955 1937 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2708 3508 4408 4594 4803 5191 2708
Size: 800 900 186 209 388 5411
Fragments arranged by size:
5411 900 800 388 209 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2198 2251 2799 3054 3171 3353 3452
Size: 393 566 53 548 255 117 182 99
Cuts at: 3452 3524 3752 3913 4490 4608 4721 5090 5132
Size: 72 228 161 577 118 113 369 42
Cuts at: 5132 5154 5957 6554 7341 7609 1239
Size: 22 803 597 787 268 1524
Fragments arranged by size:
1524 803 787 597 577 566 548 393
369 268 255 228 182 161 118 117
113 99 72 53 42 22
EcoNI CCTnn'n_nnAGG
Cuts at: 2647 2651 4829 2647
Size: 4 2178 5712
Fragments arranged by size:
5712 2178 4
EcoO109I rG'GnC_Cy
Cuts at: 2065 2298 2542 3219 3352 3364 3468 3498 3499
Size: 233 244 677 133 12 104 30 1
Cuts at: 3499 3903 4514 4515 4525 4822 4966 5052 2065
Size: 404 611 1 10 297 144 86 4907
Fragments arranged by size:
4907 677 611 404 297 244 233 144
133 104 86 30 12 10 1 1
EcoRI G'AATT_C
Cuts at: 5203 5581 5203
Size: 378 7516
FspI TGC'GCA
Cuts at: 3633 6400 3633
Size: 2767 5127
HaeII r_GCGC'y
Cuts at: 1678 2155 4668 4690 4711 7041 1678
Size: 477 2513 22 21 2330 2531
Fragments arranged by size:
2531 2513 2330 477 22 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2103 2135 2189 2221 2744
Size: 32 135 32 531 32 54 32 523
Cuts at: 2744 2776 2847 2879 3175 3207 4005 4037 4152
Size: 32 71 32 296 32 798 32 115
Cuts at: 4152 4184 4304 4336 6535 6567 6609 6641 1373
Size: 32 120 32 2199 32 42 32 2626
Fragments arranged by size:
2626 2199 798 531 523 296 135 120
115 71 54 42 32 32 32 32
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2836 5238 5484 669
Size: 988 281 898 2402 246 3079
Fragments arranged by size:
3079 2402 988 898 281 246
HindIII A'AGCT_T
Cuts at: 748 2781 5093 748
Size: 2033 2312 3549
Fragments arranged by size:
3549 2312 2033
HpaI GTT'AAC
Cuts at: 5484 5484
Size: 7894
KasI G'GCGC_C
Cuts at: 4686 4686
Size: 7894
KpnI G_GTAC'C
Cuts at: 2347 5219 2347
Size: 2872 5022
MfeI C'AATT_G
Cuts at: 5493 5493
Size: 7894
MluI A'CGCG_T
Cuts at: 7770 7770
Size: 7894
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 7008 7192 1305
Size: 774 4929 184 2007
Fragments arranged by size:
4929 2007 774 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2469 2486 2901 4091 10
Size: 54 1425 343 637 17 415 1190 3813
Fragments arranged by size:
3813 1425 1190 637 415 343 54 17
MslI CAynn'nnrTG
Cuts at: 518 1166 2876 2936 3893 5852 6211 6370 518
Size: 648 1710 60 957 1959 359 159 2042
Fragments arranged by size:
2042 1959 1710 957 648 359 159 60
MspA1I CmG'CkG
Cuts at: 1606 2701 3453 3583 3695 3922 5110 5155 5310
Size: 1095 752 130 112 227 1188 45 155
Cuts at: 5310 5987 7135 7380 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1188 1148 1095 752 677 245 227
155 130 112 45
NarI GG'CG_CC
Cuts at: 4687 4687
Size: 7894
NcoI C'CATG_G
Cuts at: 513 1065 2107 2748 3381 3951 4177 513
Size: 552 1042 641 633 570 226 4230
Fragments arranged by size:
4230 1042 641 633 570 552 226
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7894
NotI GC'GGCC_GC
Cuts at: 5341 5341
Size: 7894
NruI TCG'CGA
Cuts at: 3070 3070
Size: 7894
NspI r_CATG'y
Cuts at: 1132 1229 3161 5252 6797 1132
Size: 97 1932 2091 1545 2229
Fragments arranged by size:
2229 2091 1932 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3793 3991 4510 4849 4858 5177 1348
Size: 2445 198 519 339 9 319 4065
Fragments arranged by size:
4065 2445 519 339 319 198 9
PciI A'CATG_T
Cuts at: 3157 6793 3157
Size: 3636 4258
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3382 3463 1108
Size: 2274 81 5539
Fragments arranged by size:
5539 2274 81
PfoI T'CCnGG_A
Cuts at: 2123 2123
Size: 7894
PmeI GTTT'AAAC
Cuts at: 5198 5198
Size: 7894
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5963 5995 7476 7508 5963
Size: 32 1481 32 6349
Fragments arranged by size:
6349 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2542 3903 4525 5052 2542
Size: 1361 622 527 5384
Fragments arranged by size:
5384 1361 622 527
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7894
PsiI TTA'TAA
Cuts at: 5464 5464
Size: 7894
PspOMI G'GGCC_C
Cuts at: 2297 3219 3468 3498 4514 4917 2297
Size: 922 249 30 1016 403 5274
Fragments arranged by size:
5274 1016 922 403 249 30
PstI C_TGCA'G
Cuts at: 830 2215 3297 3401 4050 4084 4105 4673 4800
Size: 1385 1082 104 649 34 21 568 127
Cuts at: 4800 4965 5246 830
Size: 165 281 3478
Fragments arranged by size:
3478 1385 1082 649 568 281 165 127
104 34 21
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7894
PvuII CAG'CTG
Cuts at: 2701 3583 3922 5110 5155 2701
Size: 882 339 1188 45 5440
Fragments arranged by size:
5440 1188 882 339 45
SacI G_AGCT'C
Cuts at: 2710 3510 4410 4596 4805 5193 2710
Size: 800 900 186 209 388 5411
Fragments arranged by size:
5411 900 800 388 209 186
SalI G'TCGA_C
Cuts at: 1936 5236 1936
Size: 3300 4594
SanDI GG'GwC_CC
Cuts at: 2542 3903 2542
Size: 1361 6533
SapI GCTCTTCn'nnn_
Cuts at: 4266 4584 4266
Size: 318 7576
SbfI CC_TGCA'GG
Cuts at: 5246 5246
Size: 7894
ScaI AGT'ACT
Cuts at: 6142 7854 6142
Size: 1712 6182
SexAI A'CCwGG_T
Cuts at: 3612 3612
Size: 7894
SfcI C'TryA_G
Cuts at: 826 1045 2211 2406 3293 3397 4046 4080 4101
Size: 219 1166 195 887 104 649 34 21
Cuts at: 4101 4669 4796 4961 5242 6377 7058 7249 826
Size: 568 127 165 281 1135 681 191 1471
Fragments arranged by size:
1471 1166 1135 887 681 649 568 281
219 195 191 165 127 104 34 21
SfoI GGC'GCC
Cuts at: 4688 4688
Size: 7894
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7894
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7894
SmaI CCC'GGG
Cuts at: 1872 3320 4718 5221 1872
Size: 1448 1398 503 4545
Fragments arranged by size:
4545 1448 1398 503
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3232 4944 5998 6899 7161 820
Size: 197 926 1289 1712 1054 901 262 1553
Fragments arranged by size:
1712 1553 1289 1054 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5685 493
Size: 5192 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7894
SphI G_CATG'C
Cuts at: 1132 5252 1132
Size: 4120 3774
SspI AAT'ATT
Cuts at: 5 52 5818 5
Size: 47 5766 2081
Fragments arranged by size:
5766 2081 47
StuI AGG'CCT
Cuts at: 1517 2228 2236 2642 4618 4835 1517
Size: 711 8 406 1976 217 4576
Fragments arranged by size:
4576 1976 711 406 217 8
StyI C'CwwG_G
Cuts at: 513 1065 2107 2748 3381 3480 3951 4177 5333
Size: 552 1042 641 633 99 471 226 1156
Cuts at: 5333 513
Size: 3074
Fragments arranged by size:
3074 1156 1042 641 633 552 471 226
99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 3643 3868 5921 5938 6091 6276
Size: 1255 1122 941 225 2053 17 153 185
Cuts at: 6276 6721 325
Size: 445 1498
Fragments arranged by size:
2053 1498 1255 1122 941 445 225 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6140 7852 96
Size: 274 80 33 51 1443 4163 1712 138
Fragments arranged by size:
4163 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2052 2084 2119 2151 2723 2755 3533 3565 7854
Size: 32 35 32 572 32 778 32 4289
Cuts at: 7854 7886 2052
Size: 32 2060
Fragments arranged by size:
4289 2060 778 572 35 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7894
XbaI T'CTAG_A
Cuts at: 5230 5230
Size: 7894
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7894
XmaI C'CCGG_G
Cuts at: 1870 3318 4716 5219 1870
Size: 1448 1398 503 4545
Fragments arranged by size:
4545 1448 1398 503
XmnI GAAnn'nnTTC
Cuts at: 6023 6023
Size: 7894
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4462 5702 276
Size: 53 83 186 936 2928 1240 2468
Fragments arranged by size:
2928 2468 1240 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NsiI
PacI PmlI PspXI PsrI RsrII SacII SfiI SrfI
SwaI XcmI