Restriction map for
pFN21AA1880
(Circular) (Six-base) MAPSORT of: pFN21AA1880.seq Check: 6812 from: 1 to: 6541
pFN21AA1880 6541 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2118 4351 278
Size: 53 83 186 936 582 2233 2468
Fragments arranged by size:
2468 2233 936 582 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3884 1937
Size: 1947 4594
Acc65I G'GTAC_C
Cuts at: 3862 3862
Size: 6541
AclI AA'CG_TT
Cuts at: 1563 2140 4668 5041 1563
Size: 577 2528 373 3063
Fragments arranged by size:
3063 2528 577 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2247 2712 4604 5988 6256 1632
Size: 615 465 1892 1384 268 1917
Fragments arranged by size:
1917 1892 1384 615 465 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6344
AflIII A'CryG_T
Cuts at: 1203 3389 5440 6417 1203
Size: 2186 2051 977 1327
Fragments arranged by size:
2186 2051 1327 977
AgeI A'CCGG_T
Cuts at: 5372 5372
Size: 6541
AhdI GACnn_n'nnGTC
Cuts at: 5270 5270
Size: 6541
AleI CACnn'nnGTG
Cuts at: 1166 2123 1166
Size: 957 5584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2191 1184
Size: 1007 5534
AlwNI CAG_nnn'CTG
Cuts at: 4284 5856 4284
Size: 1572 4969
ApaLI G'TGCA_C
Cuts at: 2060 4598 5754 2060
Size: 2538 1156 2847
Fragments arranged by size:
2847 2538 1156
ApoI r'AATT_y
Cuts at: 1493 2235 3850 4076 4228 6337 1493
Size: 742 1615 226 152 2109 1697
Fragments arranged by size:
2109 1697 1615 742 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5095 160
Size: 872 4063 1606
Fragments arranged by size:
4063 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
AvaI C'yCGr_G
Cuts at: 1870 1943 3866 1870
Size: 73 1923 4545
Fragments arranged by size:
4545 1923 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3005 3038 3005
Size: 33 6508
BamHI G'GATC_C
Cuts at: 3871 4238 3871
Size: 367 6174
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2175 2937 3772 3862
Size: 325 217 627 297 91 762 835 90
Cuts at: 3862 5317 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 835 762 627 325 297 217
91 90
BanII G_rGCy'C
Cuts at: 1391 2857 3860 1391
Size: 1466 1003 4072
Fragments arranged by size:
4072 1466 1003
BbeI G_GCGC'C
Cuts at: 3776 3776
Size: 6541
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5570
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3743 3777 4732 4766 1903
Size: 34 1806 34 955 34 3678
Fragments arranged by size:
3678 1806 955 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3006 4433 5643 3006
Size: 1427 1210 3904
Fragments arranged by size:
3904 1427 1210
BclI T'GATC_A
Cuts at: 1548 2053 1548
Size: 505 6036
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2964 5152 136
Size: 107 122 71 631 605 1292 2188 1525
Fragments arranged by size:
2188 1525 1292 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6536 6536
Size: 6541
BlpI GC'TnA_GC
Cuts at: 3958 3958
Size: 6541
Bme1580I G_kGCm'C
Cuts at: 1790 2064 4602 5758 1790
Size: 274 2538 1156 2573
Fragments arranged by size:
2573 2538 1156 274
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6541
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2304 3382 4272
Size: 416 53 83 399 24 887 1078 890
Cuts at: 4272 5230 442
Size: 958 1753
Fragments arranged by size:
1753 1078 958 890 887 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6541
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2370 2831 2370
Size: 461 6080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3781 5201 1239
Size: 2542 1420 2579
Fragments arranged by size:
2579 2542 1420
Bpu10I CC'TnA_GC
Cuts at: 2511 2511
Size: 6541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2509 3272 4666 5531 5829 2509
Size: 763 1394 865 298 3221
Fragments arranged by size:
3221 1394 865 763 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5204 882
Size: 617 3705 2219
Fragments arranged by size:
3705 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4332 493
Size: 711 3128 2702
Fragments arranged by size:
3128 2702 711
BsaBI GATnn'nnATC
Cuts at: 2919 6535 2919
Size: 3616 2925
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2115 3773 4348 4730
Size: 53 83 186 936 582 1658 575 382
Cuts at: 4730 5365 275
Size: 635 1451
Fragments arranged by size:
1658 1451 936 635 582 575 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4974 5372 5646 5793 6204 4974
Size: 398 274 147 411 5311
Fragments arranged by size:
5311 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2844 2874 3803 3833 1178
Size: 30 165 30 1441 30 929 30 3886
Fragments arranged by size:
3886 1441 929 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 1186
Size: 1153 5388
BseYI C'CCAG_C
Cuts at: 5744 6240 5744
Size: 496 6045
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2389 1163
Size: 573 653 5315
Fragments arranged by size:
5315 653 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3991 4752 5780 1540
Size: 275 186 1990 761 1028 2301
Fragments arranged by size:
2301 1990 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2064 4602 4687 5758 2064
Size: 2538 85 1071 2847
Fragments arranged by size:
2847 2538 1071 85
BsmI GAATG_Cn'
Cuts at: 2214 2264 2512 4052 4145 2214
Size: 50 248 1540 93 4610
Fragments arranged by size:
4610 1540 248 93 50
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2857 3860 4602 4687 5758 1391
Size: 399 274 793 1003 742 85 1071 2174
Fragments arranged by size:
2174 1071 1003 793 742 399 274 85
BspEI T'CCGG_A
Cuts at: 6204 6204
Size: 6541
BspHI T'CATG_A
Cuts at: 2691 4430 6160 2691
Size: 1739 1730 3072
Fragments arranged by size:
3072 1739 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3147 3896 844
Size: 2303 749 3489
Fragments arranged by size:
3489 2303 749
BsrBI CCG'CTC
Cuts at: 1394 1823 4428 1394
Size: 429 2605 3507
Fragments arranged by size:
3507 2605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2832 5036 5210 65
Size: 1183 1584 2204 174 1396
Fragments arranged by size:
2204 1584 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3036 3740 3828 5185 5372 1700
Size: 110 1226 704 88 1357 187 2869
Fragments arranged by size:
2869 1357 1226 704 187 110 88
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6541
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6541
BssSI C'ACGA_G
Cuts at: 4601 5613 4601
Size: 1012 5529
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2677 3293 794
Size: 1883 616 4042
Fragments arranged by size:
4042 1883 616
BstBI TT'CG_AA
Cuts at: 3166 5345 3166
Size: 2179 4362
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6541
BstYI r'GATC_y
Cuts at: 1972 3871 4238 4623 4640 6092 6178 6190 6536
Size: 1899 367 385 17 1452 86 12 346
Cuts at: 6536 1972
Size: 1977
Fragments arranged by size:
1977 1899 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 3273 3534 513
Size: 552 2208 261 3520
Fragments arranged by size:
3520 2208 552 261
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5802
Fragments arranged by size:
5802 683 56
BtsI GCAGTG_nn'
Cuts at: 2046 2253 4057 4851 4871 2046
Size: 207 1804 794 20 3716
Fragments arranged by size:
3716 1804 794 207 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6541
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6506
DraI TTT'AAA
Cuts at: 2565 3845 2565
Size: 1280 5261
DraIII CAC_nnn'GTG
Cuts at: 3211 3211
Size: 6541
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5548 809
Size: 507 4232 1802
Fragments arranged by size:
4232 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3738 3988 4877
Size: 54 1425 50 275 18 1908 250 889
Cuts at: 4877 6506 8
Size: 1629 43
Fragments arranged by size:
1908 1629 1425 889 275 250 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3988 1537
Size: 275 2176 4090
Fragments arranged by size:
4090 2176 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 4471 1353
Size: 675 2443 3423
Fragments arranged by size:
3423 2443 675
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5128 5502 5648 387
Size: 1064 138 3539 374 146 1280
Fragments arranged by size:
3539 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2247 2712 3781 4604 5201 5988 6256
Size: 393 615 465 1069 823 597 787 268
Cuts at: 6256 1239
Size: 1524
Fragments arranged by size:
1524 1069 823 787 615 597 465 393
268
EcoRI G'AATT_C
Cuts at: 3850 4228 3850
Size: 378 6163
FspI TGC'GCA
Cuts at: 5047 5047
Size: 6541
HaeII r_GCGC'y
Cuts at: 1678 3776 5688 1678
Size: 2098 1912 2531
Fragments arranged by size:
2531 2098 1912
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2375 2407 2864 2896 3023
Size: 32 135 32 803 32 457 32 127
Cuts at: 3023 3055 5182 5214 5256 5288 1373
Size: 32 2127 32 42 32 2626
Fragments arranged by size:
2626 2127 803 457 135 127 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2475 3885 4131 669
Size: 988 281 537 1410 246 3079
Fragments arranged by size:
3079 1410 988 537 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6541
HpaI GTT'AAC
Cuts at: 2475 4131 2475
Size: 1656 4885
KasI G'GCGC_C
Cuts at: 3772 3772
Size: 6541
KpnI G_GTAC'C
Cuts at: 3866 3866
Size: 6541
MfeI C'AATT_G
Cuts at: 2943 3291 4140 2943
Size: 348 849 5344
Fragments arranged by size:
5344 849 348
MluI A'CGCG_T
Cuts at: 6417 6417
Size: 6541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3308 3834 5655 5839 1305
Size: 2003 526 1821 184 2007
Fragments arranged by size:
2007 2003 1821 526 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4719
Fragments arranged by size:
4719 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2082 2123 2361 2495 2768 3059 4499
Size: 648 916 41 238 134 273 291 1440
Cuts at: 4499 4858 5017 518
Size: 359 159 2042
Fragments arranged by size:
2042 1440 916 648 359 291 273 238
159 134 41
MspA1I CmG'CkG
Cuts at: 1606 3957 4634 5782 6027 1606
Size: 2351 677 1148 245 2120
Fragments arranged by size:
2351 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 3773 3773
Size: 6541
NcoI C'CATG_G
Cuts at: 513 1065 3273 3534 513
Size: 552 2208 261 3520
Fragments arranged by size:
3520 2208 552 261
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6541
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6541
NotI GC'GGCC_GC
Cuts at: 3988 3988
Size: 6541
NspI r_CATG'y
Cuts at: 1132 1229 2494 2815 3393 3899 5444 1132
Size: 97 1265 321 578 506 1545 2229
Fragments arranged by size:
2229 1545 1265 578 506 321 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6541
PciI A'CATG_T
Cuts at: 3389 5440 3389
Size: 2051 4490
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6541
PfoI T'CCnGG_A
Cuts at: 2754 2754
Size: 6541
PmeI GTTT'AAAC
Cuts at: 3845 3845
Size: 6541
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4610 4642 6123 6155 4610
Size: 32 1481 32 4996
Fragments arranged by size:
4996 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2737 1613
Size: 1124 5417
PsiI TTA'TAA
Cuts at: 4111 4111
Size: 6541
PstI C_TGCA'G
Cuts at: 830 2041 2260 3158 3893 830
Size: 1211 219 898 735 3478
Fragments arranged by size:
3478 1211 898 735 219
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6541
SalI G'TCGA_C
Cuts at: 1936 3883 1936
Size: 1947 4594
SbfI CC_TGCA'GG
Cuts at: 3893 3893
Size: 6541
ScaI AGT'ACT
Cuts at: 4789 6501 4789
Size: 1712 4829
SfcI C'TryA_G
Cuts at: 826 1045 2037 2256 3154 3490 3889 5024 5705
Size: 219 992 219 898 336 399 1135 681
Cuts at: 5705 5896 826
Size: 191 1471
Fragments arranged by size:
1471 1135 992 898 681 399 336 219
219 191
SfoI GGC'GCC
Cuts at: 3774 3774
Size: 6541
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6541
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6541
SmaI CCC'GGG
Cuts at: 1872 3868 1872
Size: 1996 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2524 3251 4645 5546 5808 820
Size: 197 926 581 727 1394 901 262 1553
Fragments arranged by size:
1553 1394 926 901 727 581 262 197
SnaBI TAC'GTA
Cuts at: 493 4332 493
Size: 3839 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6541
SphI G_CATG'C
Cuts at: 1132 2815 3899 1132
Size: 1683 1084 3774
Fragments arranged by size:
3774 1683 1084
SspI AAT'ATT
Cuts at: 5 52 2183 4465 5
Size: 47 2131 2282 2081
Fragments arranged by size:
2282 2131 2081 47
StuI AGG'CCT
Cuts at: 1517 2725 1517
Size: 1208 5333
StyI C'CwwG_G
Cuts at: 513 1065 3273 3534 3980 513
Size: 552 2208 261 446 3074
Fragments arranged by size:
3074 2208 552 446 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2955 3042 4568 4585 4738 4923 5368
Size: 1255 1375 87 1526 17 153 185 445
Cuts at: 5368 325
Size: 1498
Fragments arranged by size:
1526 1498 1375 1255 445 185 153 87
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4787 6499 96
Size: 274 80 33 51 1443 2810 1712 138
Fragments arranged by size:
2810 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6501 6533 6501
Size: 32 6509
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6541
XbaI T'CTAG_A
Cuts at: 3877 3877
Size: 6541
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3404 3404
Size: 6541
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6541
XmaI C'CCGG_G
Cuts at: 1870 3866 1870
Size: 1996 4545
XmnI GAAnn'nnTTC
Cuts at: 4670 4670
Size: 6541
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2116 4349 276
Size: 53 83 186 936 582 2233 2468
Fragments arranged by size:
2468 2233 936 582 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbvCI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII
SacI SacII SanDI SapI SexAI SfiI SrfI SwaI