Restriction map for
pFN21AA1867
(Circular) (Six-base) MAPSORT of: pFN21AA1867.seq Check: 8837 from: 1 to: 7039
pFN21AA1867 7039 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2519 2519
Size: 7039
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2726 2997 4849 278
Size: 53 83 186 936 1190 271 1852 2468
Fragments arranged by size:
2468 1852 1190 936 271 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2997 4306 4382 1937
Size: 1060 1309 76 4594
Fragments arranged by size:
4594 1309 1060 76
Acc65I G'GTAC_C
Cuts at: 4360 4360
Size: 7039
AclI AA'CG_TT
Cuts at: 1563 3378 5166 5539 1563
Size: 1815 1788 373 3063
Fragments arranged by size:
3063 1815 1788 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2035 2137 2360 3049 3914 5102 6486 6754
Size: 403 102 223 689 865 1188 1384 268
Cuts at: 6754 1632
Size: 1917
Fragments arranged by size:
1917 1384 1188 865 689 403 268 223
102
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6842
AflIII A'CryG_T
Cuts at: 1203 2919 3192 5938 6915 1203
Size: 1716 273 2746 977 1327
Fragments arranged by size:
2746 1716 1327 977 273
AgeI A'CCGG_T
Cuts at: 5870 5870
Size: 7039
AhdI GACnn_n'nnGTC
Cuts at: 5768 5768
Size: 7039
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7039
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2385 3485 1184
Size: 1201 1100 4738
Fragments arranged by size:
4738 1201 1100
AlwNI CAG_nnn'CTG
Cuts at: 2302 3596 3767 3892 4782 6354 2302
Size: 1294 171 125 890 1572 2987
Fragments arranged by size:
2987 1572 1294 890 171 125
ApaI G_GGCC'C
Cuts at: 2471 3009 2471
Size: 538 6501
ApaLI G'TGCA_C
Cuts at: 5096 6252 5096
Size: 1156 5883
ApoI r'AATT_y
Cuts at: 1493 3824 4348 4574 4726 6835 1493
Size: 2331 524 226 152 2109 1697
Fragments arranged by size:
2331 2109 1697 524 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5593 160
Size: 872 4561 1606
Fragments arranged by size:
4561 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7039
AvaI C'yCGr_G
Cuts at: 1870 1943 4364 1870
Size: 73 2421 4545
Fragments arranged by size:
4545 2421 73
BamHI G'GATC_C
Cuts at: 4369 4736 4369
Size: 367 6672
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2437 3208 4360 5815 618
Size: 325 217 627 650 771 1152 1455 1842
Fragments arranged by size:
1842 1455 1152 771 650 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2471 2937 3009 3053 3122 4358 1391
Size: 1080 466 72 44 69 1236 4072
Fragments arranged by size:
4072 1236 1080 466 72 69 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3152 928
Size: 971 1253 4815
Fragments arranged by size:
4815 1253 971
BbvCI CC'TCA_GC
Cuts at: 2978 4151 2978
Size: 1173 5866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5230 5264 1903
Size: 34 3293 34 3678
Fragments arranged by size:
3678 3293 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4931 6141 4931
Size: 1210 5829
BclI T'GATC_A
Cuts at: 1548 2475 1548
Size: 927 6112
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2396 4247 5650
Size: 107 122 71 631 605 724 1851 1403
Cuts at: 5650 136
Size: 1525
Fragments arranged by size:
1851 1525 1403 724 631 605 122 107
71
BglII A'GATC_T
Cuts at: 7034 7034
Size: 7039
BlpI GC'TnA_GC
Cuts at: 4456 4456
Size: 7039
Bme1580I G_kGCm'C
Cuts at: 1790 2471 3009 3213 3395 3688 4014 5100 6256
Size: 681 538 204 182 293 326 1086 1156
Cuts at: 6256 1790
Size: 2573
Fragments arranged by size:
2573 1156 1086 681 538 326 293 204
182
BmgBI CAC'GTC
Cuts at: 1318 2659 4336 1318
Size: 1341 1677 4021
Fragments arranged by size:
4021 1677 1341
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2839 4270 4296
Size: 416 53 83 399 24 1422 1431 26
Cuts at: 4296 4770 5728 442
Size: 474 958 1753
Fragments arranged by size:
1753 1431 1422 958 474 416 399 83
53 26 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 3805 3961 4166 2956
Size: 849 156 205 5829
Fragments arranged by size:
5829 849 205 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2810 2910 3256 3404 3875 3952 5699
Size: 815 756 100 346 148 471 77 1747
Cuts at: 5699 1239
Size: 2579
Fragments arranged by size:
2579 1747 815 756 471 346 148 100
77
Bpu10I CC'TnA_GC
Cuts at: 2978 3134 4049 4151 2978
Size: 156 915 102 5866
Fragments arranged by size:
5866 915 156 102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2269 3530 5164 6029 6327 2269
Size: 1261 1634 865 298 2981
Fragments arranged by size:
2981 1634 1261 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3410 5702 882
Size: 617 1911 2292 2219
Fragments arranged by size:
2292 2219 1911 617
BsaAI yAC'GTr
Cuts at: 493 1204 2922 3193 4830 493
Size: 711 1718 271 1637 2702
Fragments arranged by size:
2702 1718 1637 711 271
BsaBI GATnn'nnATC
Cuts at: 7033 7033
Size: 7039
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2723 2994 3438 4846
Size: 53 83 186 936 1190 271 444 1408
Cuts at: 4846 5228 5863 275
Size: 382 635 1451
Fragments arranged by size:
1451 1408 1190 936 635 444 382 271
186 83 53
BsaWI w'CCGG_w
Cuts at: 3122 3330 5472 5870 6144 6291 6702 3122
Size: 208 2142 398 274 147 411 3459
Fragments arranged by size:
3459 2142 411 398 274 208 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3624 3654 3866 3896 4148
Size: 30 165 30 2221 30 212 30 252
Cuts at: 4148 4178 1178
Size: 30 4039
Fragments arranged by size:
4039 2221 252 212 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3446 3797 3887 4252 1186
Size: 2260 351 90 365 3973
Fragments arranged by size:
3973 2260 365 351 90
BseYI C'CCAG_C
Cuts at: 2062 2095 2833 3285 4093 6242 6738 2062
Size: 33 738 452 808 2149 496 2363
Fragments arranged by size:
2363 2149 808 738 496 452 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3484 4247 1163
Size: 573 1748 763 3955
Fragments arranged by size:
3955 1748 763 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4489 5250 6278 1540
Size: 275 186 2488 761 1028 2301
Fragments arranged by size:
2488 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2334 3338 3789 3942 5100 5185 6256 2334
Size: 1004 451 153 1158 85 1071 3117
Fragments arranged by size:
3117 1158 1071 1004 451 153 85
BsmI GAATG_Cn'
Cuts at: 4550 4643 4550
Size: 93 6946
BsmBI CGTCTCn'nnnn_
Cuts at: 2714 2944 2714
Size: 230 6809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2334 2471 2937 3009 3053 3122 3213
Size: 399 544 137 466 72 44 69 91
Cuts at: 3213 3338 3395 3688 3789 3942 4014 4358 5100
Size: 125 57 293 101 153 72 344 742
Cuts at: 5100 5185 6256 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 544 466 399 344 293
153 137 125 101 91 85 72 72
69 57 44
BspEI T'CCGG_A
Cuts at: 3122 3330 6702 3122
Size: 208 3372 3459
Fragments arranged by size:
3459 3372 208
BspHI T'CATG_A
Cuts at: 4928 6658 4928
Size: 1730 5309
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2519 3993 4394 844
Size: 1675 1474 401 3489
Fragments arranged by size:
3489 1675 1474 401
BsrBI CCG'CTC
Cuts at: 1394 1823 2409 4053 4129 4926 1394
Size: 429 586 1644 76 797 3507
Fragments arranged by size:
3507 1644 797 586 429 76
BsrDI GCAATG_nn'
Cuts at: 65 1248 3145 5534 5708 65
Size: 1183 1897 2389 174 1396
Fragments arranged by size:
2389 1897 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3080 5683 5870 1700
Size: 110 1270 2603 187 2869
Fragments arranged by size:
2869 2603 1270 187 110
BsrGI T'GTAC_A
Cuts at: 96 2898 96
Size: 2802 4237
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7039
BssSI C'ACGA_G
Cuts at: 3038 5099 6111 3038
Size: 2061 1012 3966
Fragments arranged by size:
3966 2061 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2525 4009 794
Size: 1731 1484 3824
Fragments arranged by size:
3824 1731 1484
BstBI TT'CG_AA
Cuts at: 5843 5843
Size: 7039
BstEII G'GTnAC_C
Cuts at: 1184 2728 2950 3238 1184
Size: 1544 222 288 4985
Fragments arranged by size:
4985 1544 288 222
BstXI CCAn_nnnn'nTGG
Cuts at: 2962 2962
Size: 7039
BstYI r'GATC_y
Cuts at: 1972 2343 3043 4369 4736 5121 5138 6590 6676
Size: 371 700 1326 367 385 17 1452 86
Cuts at: 6676 6688 7034 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1326 700 385 371 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2348 2348
Size: 7039
BtgI C'CryG_G
Cuts at: 513 1065 3298 513
Size: 552 2233 4254
Fragments arranged by size:
4254 2233 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3340 487
Size: 683 56 2114 4186
Fragments arranged by size:
4186 2114 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 4187 4555 5349 5369 2812
Size: 1375 368 794 20 4482
Fragments arranged by size:
4482 1375 794 368 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7039
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3651 3686 529
Size: 35 3087 35 3882
Fragments arranged by size:
3882 3087 35 35
DraI TTT'AAA
Cuts at: 4343 4343
Size: 7039
DraIII CAC_nnn'GTG
Cuts at: 4182 4182
Size: 7039
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2801 4193 6046 809
Size: 507 1485 1392 1853 1802
Fragments arranged by size:
1853 1802 1485 1392 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2106 2668 3606
Size: 54 1425 50 275 18 276 562 938
Cuts at: 3606 3624 4054 4486 5375 7004 8
Size: 18 430 432 889 1629 43
Fragments arranged by size:
1629 1425 938 889 562 432 430 276
275 54 50 43 18 18
EagI C'GGCC_G
Cuts at: 1537 1812 4486 1537
Size: 275 2674 4090
Fragments arranged by size:
4090 2674 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2054 2069 2645 3864 4969 1353
Size: 683 18 15 576 1219 1105 3423
Fragments arranged by size:
3423 1219 1105 683 576 18 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 5626 6000 6146 387
Size: 1064 138 2747 1290 374 146 1280
Fragments arranged by size:
2747 1290 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2054 2137 2360 2810 2910 3049
Size: 393 403 19 83 223 450 100 139
Cuts at: 3049 3256 3404 3875 3914 3952 5102 5699 6486
Size: 207 148 471 39 38 1150 597 787
Cuts at: 6486 6754 1239
Size: 268 1524
Fragments arranged by size:
1524 1150 787 597 471 450 403 393
268 223 207 148 139 100 83 39
38 19
EcoO109I rG'GnC_Cy
Cuts at: 2278 2467 2468 2494 3006 3859 3898 2278
Size: 189 1 26 512 853 39 5419
Fragments arranged by size:
5419 853 512 189 39 26 1
EcoRI G'AATT_C
Cuts at: 3824 4348 4726 3824
Size: 524 378 6137
Fragments arranged by size:
6137 524 378
EcoRV GAT'ATC
Cuts at: 3733 3733
Size: 7039
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2272 2272
Size: 7039
FspI TGC'GCA
Cuts at: 5545 5545
Size: 7039
HaeII r_GCGC'y
Cuts at: 1678 3406 6186 1678
Size: 1728 2780 2531
Fragments arranged by size:
2780 2531 1728
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2556 2588 3035 3067 3249
Size: 32 135 32 984 32 447 32 182
Cuts at: 3249 3281 4148 4180 4315 4347 5680 5712 5754
Size: 32 867 32 135 32 1333 32 42
Cuts at: 5754 5786 1373
Size: 32 2626
Fragments arranged by size:
2626 1333 984 867 447 182 135 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2761 2992 3925 4307 4383 4629
Size: 988 281 823 231 933 382 76 246
Cuts at: 4629 669
Size: 3079
Fragments arranged by size:
3079 988 933 823 382 281 246 231
76
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7039
HpaI GTT'AAC
Cuts at: 4629 4629
Size: 7039
KpnI G_GTAC'C
Cuts at: 4364 4364
Size: 7039
MfeI C'AATT_G
Cuts at: 4638 4638
Size: 7039
MluI A'CGCG_T
Cuts at: 6915 6915
Size: 7039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3549 6153 6337 1305
Size: 2244 2604 184 2007
Fragments arranged by size:
2604 2244 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2108 2670 10
Size: 54 1425 343 276 562 4379
Fragments arranged by size:
4379 1425 562 343 276 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3620 4019 4997 5356 5515 518
Size: 648 2454 399 978 359 159 2042
Fragments arranged by size:
2454 2042 978 648 399 359 159
MspA1I CmG'CkG
Cuts at: 1606 2837 3069 3805 3853 3972 4084 4206 4318
Size: 1231 232 736 48 119 112 122 112
Cuts at: 4318 4455 5132 6280 6525 1606
Size: 137 677 1148 245 2120
Fragments arranged by size:
2120 1231 1148 736 677 245 232 137
122 119 112 112 48
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6487
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7039
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7039
NotI GC'GGCC_GC
Cuts at: 4486 4486
Size: 7039
NspI r_CATG'y
Cuts at: 1132 1229 4024 4397 5942 1132
Size: 97 2795 373 1545 2229
Fragments arranged by size:
2795 2229 1545 373 97
PasI CC'CwG_GG
Cuts at: 1348 2962 1348
Size: 1614 5425
PciI A'CATG_T
Cuts at: 5938 5938
Size: 7039
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2179 2550 3105 3767 1108
Size: 1071 371 555 662 4380
Fragments arranged by size:
4380 1071 662 555 371
PfoI T'CCnGG_A
Cuts at: 3365 4170 3365
Size: 805 6234
PmeI GTTT'AAAC
Cuts at: 4343 4343
Size: 7039
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5108 5140 6621 6653 4118
Size: 32 958 32 1481 32 4504
Fragments arranged by size:
4504 1481 958 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2278 2494 3859 3898 2278
Size: 216 1365 39 5419
Fragments arranged by size:
5419 1365 216 39
PshAI GACnn'nnGTC
Cuts at: 1613 2726 3101 3987 4302 4334 1613
Size: 1113 375 886 315 32 4318
Fragments arranged by size:
4318 1113 886 375 315 32
PsiI TTA'TAA
Cuts at: 4609 4609
Size: 7039
PspOMI G'GGCC_C
Cuts at: 2467 3005 2467
Size: 538 6501
PstI C_TGCA'G
Cuts at: 830 2539 4317 4391 830
Size: 1709 1778 74 3478
Fragments arranged by size:
3478 1778 1709 74
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7039
PvuII CAG'CTG
Cuts at: 2837 3069 3805 3853 3972 2837
Size: 232 736 48 119 5904
Fragments arranged by size:
5904 736 232 119 48
RsrII CG'GwC_CG
Cuts at: 3349 3349
Size: 7039
SalI G'TCGA_C
Cuts at: 1936 4305 4381 1936
Size: 2369 76 4594
Fragments arranged by size:
4594 2369 76
SanDI GG'GwC_CC
Cuts at: 2494 2494
Size: 7039
SapI GCTCTTCn'nnn_
Cuts at: 2036 2036
Size: 7039
SbfI CC_TGCA'GG
Cuts at: 4391 4391
Size: 7039
ScaI AGT'ACT
Cuts at: 5287 6999 5287
Size: 1712 5327
SexAI A'CCwGG_T
Cuts at: 2172 2301 2172
Size: 129 6910
SfcI C'TryA_G
Cuts at: 826 1045 2535 3916 4039 4313 4387 5522 6203
Size: 219 1490 1381 123 274 74 1135 681
Cuts at: 6203 6394 826
Size: 191 1471
Fragments arranged by size:
1490 1471 1381 1135 681 274 219 191
123 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 2396 2396
Size: 7039
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7039
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7039
SmaI CCC'GGG
Cuts at: 1872 4366 1872
Size: 2494 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2284 3545 5143 6044 6306 820
Size: 197 926 341 1261 1598 901 262 1553
Fragments arranged by size:
1598 1553 1261 926 901 341 262 197
SnaBI TAC'GTA
Cuts at: 493 4830 493
Size: 4337 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7039
SphI G_CATG'C
Cuts at: 1132 4397 1132
Size: 3265 3774
SspI AAT'ATT
Cuts at: 5 52 4963 5
Size: 47 4911 2081
Fragments arranged by size:
4911 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7039
StyI C'CwwG_G
Cuts at: 513 1065 2109 4478 513
Size: 552 1044 2369 3074
Fragments arranged by size:
3074 2369 1044 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2255 2716 2858 3293 5066 5083 5236
Size: 1255 675 461 142 435 1773 17 153
Cuts at: 5236 5421 5866 325
Size: 185 445 1498
Fragments arranged by size:
1773 1498 1255 675 461 445 435 185
153 142 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2847 2898 5285
Size: 274 80 33 51 1443 870 51 2387
Cuts at: 5285 6997 96
Size: 1712 138
Fragments arranged by size:
2387 1712 1443 870 274 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2181 2213 3624 3656 4166 4198 4262 4294 6999
Size: 32 1411 32 510 32 64 32 2705
Cuts at: 6999 7031 2181
Size: 32 2189
Fragments arranged by size:
2705 2189 1411 510 64 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2453 2798 3839 1316
Size: 1137 345 1041 4516
Fragments arranged by size:
4516 1137 1041 345
XbaI T'CTAG_A
Cuts at: 4375 4375
Size: 7039
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7039
XmaI C'CCGG_G
Cuts at: 1870 4364 1870
Size: 2494 4545
XmnI GAAnn'nnTTC
Cuts at: 2012 3515 5168 2012
Size: 1503 1653 3883
Fragments arranged by size:
3883 1653 1503
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2724 2995 4847 276
Size: 53 83 186 936 1190 271 1852 2468
Fragments arranged by size:
2468 1852 1190 936 271 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII RsrII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoICRI EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI
SacII SfoI SrfI SwaI XcmI