Restriction map for
pFN21AA1861
(Circular) (Six-base) MAPSORT of: pFN21AA1861.seq Check: 5648 from: 1 to: 7576
pFN21AA1861 7576 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5386 278
Size: 53 83 186 936 3850 2468
Fragments arranged by size:
3850 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3994 4537 4919 1937
Size: 2057 543 382 4594
Fragments arranged by size:
4594 2057 543 382
Acc65I G'GTAC_C
Cuts at: 4897 4897
Size: 7576
AclI AA'CG_TT
Cuts at: 1563 5703 6076 1563
Size: 4140 373 3063
Fragments arranged by size:
4140 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3033 3078 3102 3306 4205 4253 5639 7023
Size: 1401 45 24 204 899 48 1386 1384
Cuts at: 7023 7291 1632
Size: 268 1917
Fragments arranged by size:
1917 1401 1386 1384 899 268 204 48
45 24
AflII C'TTAA_G
Cuts at: 820 1017 3866 820
Size: 197 2849 4530
Fragments arranged by size:
4530 2849 197
AflIII A'CryG_T
Cuts at: 1203 2926 6475 7452 1203
Size: 1723 3549 977 1327
Fragments arranged by size:
3549 1723 1327 977
AgeI A'CCGG_T
Cuts at: 6407 6407
Size: 7576
AhdI GACnn_n'nnGTC
Cuts at: 6305 6305
Size: 7576
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7576
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2983 3266 4520 1184
Size: 1799 283 1254 4240
Fragments arranged by size:
4240 1799 1254 283
AlwNI CAG_nnn'CTG
Cuts at: 2020 2482 3056 4366 5319 6891 2020
Size: 462 574 1310 953 1572 2705
Fragments arranged by size:
2705 1572 1310 953 574 462
ApaLI G'TGCA_C
Cuts at: 5633 6789 5633
Size: 1156 6420
ApoI r'AATT_y
Cuts at: 1493 2090 2650 2722 2891 3266 3412 3437 3443
Size: 597 560 72 169 375 146 25 6
Cuts at: 3443 3558 4448 4685 4885 5111 5263 7372 1493
Size: 115 890 237 200 226 152 2109 1697
Fragments arranged by size:
2109 1697 890 597 560 375 237 226
200 169 152 146 115 72 25 6
AseI AT'TA_AT
Cuts at: 160 1032 2145 6130 160
Size: 872 1113 3985 1606
Fragments arranged by size:
3985 1606 1113 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
AvaI C'yCGr_G
Cuts at: 1870 1943 3738 4901 1870
Size: 73 1795 1163 4545
Fragments arranged by size:
4545 1795 1163 73
BamHI G'GATC_C
Cuts at: 4805 4906 5273 4805
Size: 101 367 7108
Fragments arranged by size:
7108 367 101
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 4099 4897 6352 618
Size: 325 217 627 267 2045 798 1455 1842
Fragments arranged by size:
2045 1842 1455 798 627 325 267 217
BanII G_rGCy'C
Cuts at: 1391 4895 1391
Size: 3504 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2360 2410 928
Size: 971 461 50 6094
Fragments arranged by size:
6094 971 461 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5767 5801 1903
Size: 34 3830 34 3678
Fragments arranged by size:
3830 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3034 4138 5468 6678 3034
Size: 1104 1330 1210 3932
Fragments arranged by size:
3932 1330 1210 1104
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7576
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6187 136
Size: 107 122 71 631 605 4515 1525
Fragments arranged by size:
4515 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7571 7571
Size: 7576
BlpI GC'TnA_GC
Cuts at: 2993 4993 2993
Size: 2000 5576
Bme1580I G_kGCm'C
Cuts at: 1790 4609 5637 6793 1790
Size: 2819 1028 1156 2573
Fragments arranged by size:
2819 2573 1156 1028
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7576
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5307 6265 442
Size: 416 53 83 399 24 3890 958 1753
Fragments arranged by size:
3890 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7576
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2225 2576 6236 1239
Size: 986 351 3660 2579
Fragments arranged by size:
3660 2579 986 351
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2016 2109 2421 5701 6566 6864 2016
Size: 93 312 3280 865 298 2728
Fragments arranged by size:
3280 2728 865 312 298 93
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6239 882
Size: 617 4740 2219
Fragments arranged by size:
4740 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5367 493
Size: 711 4163 2702
Fragments arranged by size:
4163 2702 711
BsaBI GATnn'nnATC
Cuts at: 7570 7570
Size: 7576
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5383 5765 6400 275
Size: 53 83 186 936 3850 382 635 1451
Fragments arranged by size:
3850 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6009 6407 6681 6828 7239 6009
Size: 398 274 147 411 6346
Fragments arranged by size:
6346 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7351
Fragments arranged by size:
7351 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2573 1186
Size: 1387 6189
BseYI C'CCAG_C
Cuts at: 6779 7275 6779
Size: 496 7080
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2892 1163
Size: 573 1156 5847
Fragments arranged by size:
5847 1156 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5026 5787 6815 1540
Size: 275 186 3025 761 1028 2301
Fragments arranged by size:
3025 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4755 5637 5722 6793 4755
Size: 882 85 1071 5538
Fragments arranged by size:
5538 1071 882 85
BsmI GAATG_Cn'
Cuts at: 3796 5087 5180 3796
Size: 1291 93 6192
Fragments arranged by size:
6192 1291 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4609 4755 4895 5637 5722 6793 1391
Size: 399 2819 146 140 742 85 1071 2174
Fragments arranged by size:
2819 2174 1071 742 399 146 140 85
BspEI T'CCGG_A
Cuts at: 7239 7239
Size: 7576
BspHI T'CATG_A
Cuts at: 3433 5465 7195 3433
Size: 2032 1730 3814
Fragments arranged by size:
3814 2032 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3234 4931 844
Size: 2390 1697 3489
Fragments arranged by size:
3489 2390 1697
BsrBI CCG'CTC
Cuts at: 1394 1823 5463 1394
Size: 429 3640 3507
Fragments arranged by size:
3640 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2354 2519 2796 6071 6245 65
Size: 1183 1106 165 277 3275 174 1396
Fragments arranged by size:
3275 1396 1183 1106 277 174 165
BsrFI r'CCGG_y
Cuts at: 1700 1810 6220 6407 1700
Size: 110 4410 187 2869
Fragments arranged by size:
4410 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2390 2921 96
Size: 2294 531 4751
Fragments arranged by size:
4751 2294 531
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7576
BssSI C'ACGA_G
Cuts at: 5636 6648 5636
Size: 1012 6564
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7576
BstBI TT'CG_AA
Cuts at: 6380 6380
Size: 7576
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7576
BstXI CCAn_nnnn'nTGG
Cuts at: 4326 4326
Size: 7576
BstYI r'GATC_y
Cuts at: 1972 2914 4805 4906 5273 5658 5675 7127 7213
Size: 942 1891 101 367 385 17 1452 86
Cuts at: 7213 7225 7571 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1891 1452 942 385 367 346 101
86 17 12
BstZ17I GTA'TAC
Cuts at: 4538 4538
Size: 7576
Bsu36I CC'TnA_GG
Cuts at: 3675 3675
Size: 7576
BtgI C'CryG_G
Cuts at: 513 1065 4179 513
Size: 552 3114 3910
Fragments arranged by size:
3910 3114 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6837
Fragments arranged by size:
6837 683 56
BtsI GCAGTG_nn'
Cuts at: 5092 5886 5906 5092
Size: 794 20 6762
Fragments arranged by size:
6762 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7576
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7541
DraI TTT'AAA
Cuts at: 2623 3442 4880 2623
Size: 819 1438 5319
Fragments arranged by size:
5319 1438 819
DraIII CAC_nnn'GTG
Cuts at: 4126 4126
Size: 7576
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6583 809
Size: 507 5267 1802
Fragments arranged by size:
5267 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5023 5912 7541
Size: 54 1425 50 275 18 3193 889 1629
Cuts at: 7541 8
Size: 43
Fragments arranged by size:
3193 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 5023 1537
Size: 275 3211 4090
Fragments arranged by size:
4090 3211 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5506 1353
Size: 4153 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6163 6537 6683 387
Size: 1064 138 4574 374 146 1280
Fragments arranged by size:
4574 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2225 2576 3033 3078 3102 3306 4205
Size: 393 593 351 457 45 24 204 899
Cuts at: 4205 4253 5639 6236 7023 7291 1239
Size: 48 1386 597 787 268 1524
Fragments arranged by size:
1524 1386 899 787 597 593 457 393
351 268 204 48 45 24
EcoO109I rG'GnC_Cy
Cuts at: 2074 2074
Size: 7576
EcoRI G'AATT_C
Cuts at: 2090 2650 4885 5263 2090
Size: 560 2235 378 4403
Fragments arranged by size:
4403 2235 560 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2867 4046 2867
Size: 1179 6397
FspI TGC'GCA
Cuts at: 6082 6082
Size: 7576
HaeII r_GCGC'y
Cuts at: 1678 6723 1678
Size: 5045 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3628 3660 4797 4829 6217
Size: 32 135 32 2056 32 1137 32 1388
Cuts at: 6217 6249 6291 6323 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2056 1388 1137 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4920 5166 669
Size: 988 281 2982 246 3079
Fragments arranged by size:
3079 2982 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2210 2757 4707 748
Size: 1462 547 1950 3617
Fragments arranged by size:
3617 1950 1462 547
HpaI GTT'AAC
Cuts at: 5166 5166
Size: 7576
KpnI G_GTAC'C
Cuts at: 4901 4901
Size: 7576
MfeI C'AATT_G
Cuts at: 3873 4616 5175 3873
Size: 743 559 6274
Fragments arranged by size:
6274 743 559
MluI A'CGCG_T
Cuts at: 7452 7452
Size: 7576
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3238 3817 3929 6690 6874 1305
Size: 1933 579 112 2761 184 2007
Fragments arranged by size:
2761 2007 1933 579 184 112
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5754
Fragments arranged by size:
5754 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3033 3142 3576 4233 5534 5893 6052
Size: 648 1867 109 434 657 1301 359 159
Cuts at: 6052 518
Size: 2042
Fragments arranged by size:
2042 1867 1301 657 648 434 359 159
109
MspA1I CmG'CkG
Cuts at: 1606 2692 4052 4777 4992 5669 6817 7062 1606
Size: 1086 1360 725 215 677 1148 245 2120
Fragments arranged by size:
2120 1360 1148 1086 725 677 245 215
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7024
NdeI CA'TA_TG
Cuts at: 387 2805 387
Size: 2418 5158
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7576
NotI GC'GGCC_GC
Cuts at: 5023 5023
Size: 7576
NspI r_CATG'y
Cuts at: 1132 1229 2930 4934 6479 1132
Size: 97 1701 2004 1545 2229
Fragments arranged by size:
2229 2004 1701 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7576
PciI A'CATG_T
Cuts at: 2926 6475 2926
Size: 3549 4027
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7576
PfoI T'CCnGG_A
Cuts at: 2572 2572
Size: 7576
PmeI GTTT'AAAC
Cuts at: 4880 4880
Size: 7576
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5645 5677 7158 7190 5645
Size: 32 1481 32 6031
Fragments arranged by size:
6031 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2074 2074
Size: 7576
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7576
PsiI TTA'TAA
Cuts at: 5146 5146
Size: 7576
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4521 4553 4521
Size: 32 7544
PstI C_TGCA'G
Cuts at: 830 2281 4252 4928 830
Size: 1451 1971 676 3478
Fragments arranged by size:
3478 1971 1451 676
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7576
PvuII CAG'CTG
Cuts at: 2692 4052 4777 2692
Size: 1360 725 5491
Fragments arranged by size:
5491 1360 725
SalI G'TCGA_C
Cuts at: 1936 4918 1936
Size: 2982 4594
SanDI GG'GwC_CC
Cuts at: 2074 2074
Size: 7576
SbfI CC_TGCA'GG
Cuts at: 4928 4928
Size: 7576
ScaI AGT'ACT
Cuts at: 3533 5824 7536 3533
Size: 2291 1712 3573
Fragments arranged by size:
3573 2291 1712
SfcI C'TryA_G
Cuts at: 826 1045 2277 3535 4248 4848 4924 6059 6740
Size: 219 1232 1258 713 600 76 1135 681
Cuts at: 6740 6931 826
Size: 191 1471
Fragments arranged by size:
1471 1258 1232 1135 713 681 600 219
191 76
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7576
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7576
SmaI CCC'GGG
Cuts at: 1872 4903 1872
Size: 3031 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2031 2124 2436 3866 5680 6581
Size: 197 926 88 93 312 1430 1814 901
Cuts at: 6581 6843 820
Size: 262 1553
Fragments arranged by size:
1814 1553 1430 926 901 312 262 197
93 88
SnaBI TAC'GTA
Cuts at: 493 5367 493
Size: 4874 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7576
SphI G_CATG'C
Cuts at: 1132 4934 1132
Size: 3802 3774
SspI AAT'ATT
Cuts at: 5 52 2416 4763 5500 5
Size: 47 2364 2347 737 2081
Fragments arranged by size:
2364 2347 2081 737 47
StuI AGG'CCT
Cuts at: 1517 2451 1517
Size: 934 6642
StyI C'CwwG_G
Cuts at: 513 1065 2745 4188 5015 513
Size: 552 1680 1443 827 3074
Fragments arranged by size:
3074 1680 1443 827 552
SwaI ATTT'AAAT
Cuts at: 3442 3442
Size: 7576
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2831 5603 5620 5773 5958 6403 325
Size: 1255 1251 2772 17 153 185 445 1498
Fragments arranged by size:
2772 1498 1255 1251 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2390 2921 3531
Size: 274 80 33 51 1443 413 531 610
Cuts at: 3531 5822 7534 96
Size: 2291 1712 138
Fragments arranged by size:
2291 1712 1443 610 531 413 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7536 7568 7536
Size: 32 7544
Tth111I GACn'n_nGTC
Cuts at: 1316 2016 1316
Size: 700 6876
XbaI T'CTAG_A
Cuts at: 4039 4912 4039
Size: 873 6703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4186 4186
Size: 7576
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7576
XmaI C'CCGG_G
Cuts at: 1870 4901 1870
Size: 3031 4545
XmnI GAAnn'nnTTC
Cuts at: 5705 5705
Size: 7576
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5384 276
Size: 53 83 186 936 3850 2468
Fragments arranged by size:
3850 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI RsrII SacI SacII
SapI SexAI SfiI SfoI SrfI