Restriction map for
pFN21AA1851
(Circular) (Six-base) MAPSORT of: pFN21AA1851.seq Check: 4505 from: 1 to: 6217
pFN21AA1851 6217 bp
With 182 enzymes: SgfI *
January 16, 2009 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2541 2565 2700 2541
Size: 24 135 6058
Fragments arranged by size:
6058 135 24
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 2770 3538 2770
Size: 768 5449
AclI AA'CG_TT
Cuts at: 1563 4344 4717 1563
Size: 2781 373 3063
Fragments arranged by size:
3063 2781 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3482 4280 5664 5932 1632
Size: 1850 798 1384 268 1917
Fragments arranged by size:
1917 1850 1384 798 268
AfeI AGC'GCT
Cuts at: 2702 2702
Size: 6217
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6020
AflIII A'CryG_T
Cuts at: 1203 2361 5116 6093 1203
Size: 1158 2755 977 1327
Fragments arranged by size:
2755 1327 1158 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6217
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3011 1184
Size: 1827 4390
AlwNI CAG_nnn'CTG
Cuts at: 2230 3422 3960 5532 2230
Size: 1192 538 1572 2915
Fragments arranged by size:
2915 1572 1192 538
ApaI G_GGCC'C
Cuts at: 3075 3075
Size: 6217
ApaLI G'TGCA_C
Cuts at: 2441 3116 4274 5430 2441
Size: 675 1158 1156 3228
Fragments arranged by size:
3228 1158 1156 675
ApoI r'AATT_y
Cuts at: 1493 3428 3526 3752 3904 6013 1493
Size: 1935 98 226 152 2109 1697
Fragments arranged by size:
2109 1935 1697 226 152 98
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3137 3542 1870
Size: 73 1194 405 4545
Fragments arranged by size:
4545 1194 405 73
AvrII C'CTAG_G
Cuts at: 2152 2152
Size: 6217
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2101 2134 2762 2795 2101
Size: 33 628 33 5523
Fragments arranged by size:
5523 628 33 33
BamHI G'GATC_C
Cuts at: 2972 3547 3914 2972
Size: 575 367 5275
Fragments arranged by size:
5275 575 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2060 2248 2706 2770 2965
Size: 325 217 627 273 188 458 64 195
Cuts at: 2965 3538 4993 618
Size: 573 1455 1842
Fragments arranged by size:
1842 1455 627 573 458 325 273 217
195 188 64
BanII G_rGCy'C
Cuts at: 1391 3075 3138 3536 1391
Size: 1684 63 398 4072
Fragments arranged by size:
4072 1684 398 63
BbeI G_GCGC'C
Cuts at: 2252 2969 2252
Size: 717 5500
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2308 928
Size: 971 409 4837
Fragments arranged by size:
4837 971 409
BbvCI CC'TCA_GC
Cuts at: 3423 3423
Size: 6217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2165 2199 2279 2313 2491 2525 4408
Size: 34 228 34 80 34 178 34 1883
Cuts at: 4408 4442 1903
Size: 34 3678
Fragments arranged by size:
3678 1883 228 178 80 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4109 5319 4109
Size: 1210 5007
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3390 3399 4828
Size: 107 122 71 631 605 1718 9 1429
Cuts at: 4828 136
Size: 1525
Fragments arranged by size:
1718 1525 1429 631 605 122 107 71
9
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 2138 2480 3290 3634 2138
Size: 342 810 344 4721
Fragments arranged by size:
4721 810 344 342
Bme1580I G_kGCm'C
Cuts at: 1790 2445 3075 3120 4278 5434 1790
Size: 655 630 45 1158 1156 2573
Fragments arranged by size:
2573 1158 1156 655 630 45
BmgBI CAC'GTC
Cuts at: 1318 2167 1318
Size: 849 5368
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3078 3948 4906
Size: 416 53 83 399 24 1661 870 958
Cuts at: 4906 442
Size: 1753
Fragments arranged by size:
1753 1661 958 870 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2839 2839
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2429 3395 3468 4877 1239
Size: 968 222 966 73 1409 2579
Fragments arranged by size:
2579 1409 968 966 222 73
Bpu10I CC'TnA_GC
Cuts at: 2207 2607 2720 3423 2207
Size: 400 113 703 5001
Fragments arranged by size:
5001 703 400 113
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2618 4342 5207 5505 2618
Size: 1724 865 298 3330
Fragments arranged by size:
3330 1724 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4880 882
Size: 617 3381 2219
Fragments arranged by size:
3381 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2752 3364 4008 493
Size: 711 1548 612 644 2702
Fragments arranged by size:
2702 1548 711 644 612
BsaBI GATnn'nnATC
Cuts at: 6211 6211
Size: 6217
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2249 2313 2966 4024
Size: 53 83 186 936 716 64 653 1058
Cuts at: 4024 4406 5041 275
Size: 382 635 1451
Fragments arranged by size:
1451 1058 936 716 653 635 382 186
83 64 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2561 2591 1178
Size: 30 165 30 1158 30 4804
Fragments arranged by size:
4804 1158 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2566 1186
Size: 1380 4837
BseYI C'CCAG_C
Cuts at: 2023 2302 2398 2587 2833 3169 3272 3335 5420
Size: 279 96 189 246 336 103 63 2085
Cuts at: 5420 5916 2023
Size: 496 2324
Fragments arranged by size:
2324 2085 496 336 279 246 189 103
96 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2128 2258 2519 3065 3428 1163
Size: 573 392 130 261 546 363 3952
Fragments arranged by size:
3952 573 546 392 363 261 130
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2855 3667 4428 5456 1540
Size: 275 186 854 812 761 1028 2301
Fragments arranged by size:
2301 1028 854 812 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2445 2865 3120 3195 4278 4363 5434 2445
Size: 420 255 75 1083 85 1071 3228
Fragments arranged by size:
3228 1083 1071 420 255 85 75
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2445 2865 3075 3120 3138 3195 3536
Size: 399 655 420 210 45 18 57 341
Cuts at: 3536 4278 4363 5434 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 655 420 399 341 210
85 57 45 18
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 4106 5836 4106
Size: 1730 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 2191 2541 2565 2700 3572 844
Size: 1298 49 350 24 135 872 3489
Fragments arranged by size:
3489 1298 872 350 135 49 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2851 3280 4104 1394
Size: 429 1028 429 824 3507
Fragments arranged by size:
3507 1028 824 429 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2928 4712 4886 65
Size: 1183 1680 1784 174 1396
Fragments arranged by size:
1784 1680 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2583 2623 4861 5048 1700
Size: 110 773 40 2238 187 2869
Fragments arranged by size:
2869 2238 773 187 110 40
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 2266 2473 2475 2741 1925
Size: 341 207 2 266 5401
Fragments arranged by size:
5401 341 266 207 2
BssSI C'ACGA_G
Cuts at: 3098 3194 4277 5289 3098
Size: 96 1083 1012 4026
Fragments arranged by size:
4026 1083 1012 96
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2167 794
Size: 1373 4844
BstBI TT'CG_AA
Cuts at: 5021 5021
Size: 6217
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6217
BstXI CCAn_nnnn'nTGG
Cuts at: 2587 3169 2587
Size: 582 5635
BstYI r'GATC_y
Cuts at: 1972 2972 3547 3914 4299 4316 5768 5854 5866
Size: 1000 575 367 385 17 1452 86 12
Cuts at: 5866 6212 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1000 575 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2336 2891 2336
Size: 555 5662
BtgI C'CryG_G
Cuts at: 513 1065 2464 3180 513
Size: 552 1399 716 3550
Fragments arranged by size:
3550 1399 716 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2974 2994 487
Size: 683 56 1748 20 3710
Fragments arranged by size:
3710 1748 683 56 20
BtsI GCAGTG_nn'
Cuts at: 2030 3164 3733 4527 4547 2030
Size: 1134 569 794 20 3700
Fragments arranged by size:
3700 1134 794 569 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6182
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5224 809
Size: 507 3908 1802
Fragments arranged by size:
3908 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2377 2613 2852
Size: 54 1425 50 275 18 547 236 239
Cuts at: 2852 2919 3000 3015 3063 3400 3664 4553 6182
Size: 67 81 15 48 337 264 889 1629
Cuts at: 6182 8
Size: 43
Fragments arranged by size:
1629 1425 889 547 337 275 264 239
236 81 67 54 50 48 43 18
15
EagI C'GGCC_G
Cuts at: 1537 1812 2852 3664 1537
Size: 275 1040 812 4090
Fragments arranged by size:
4090 1040 812 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2261 2853 3056 4147 1353
Size: 908 592 203 1091 3423
Fragments arranged by size:
3423 1091 908 592 203
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3469 4804 5178 5324 387
Size: 1064 138 1880 1335 374 146 1280
Fragments arranged by size:
1880 1335 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2429 3395 3468 3482 4280 4877
Size: 393 575 222 966 73 14 798 597
Cuts at: 4877 5664 5932 1239
Size: 787 268 1524
Fragments arranged by size:
1524 966 798 787 597 575 393 268
222 73 14
EcoO109I rG'GnC_Cy
Cuts at: 2716 3072 3391 3503 2716
Size: 356 319 112 5430
Fragments arranged by size:
5430 356 319 112
EcoRI G'AATT_C
Cuts at: 3428 3526 3904 3428
Size: 98 378 5741
Fragments arranged by size:
5741 378 98
FspI TGC'GCA
Cuts at: 2927 4723 2927
Size: 1796 4421
FspAI rTGC'GCAy
Cuts at: 2927 2927
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 2230 2252 2375 2408 2550 2704 2727 2969
Size: 552 22 123 33 142 154 23 242
Cuts at: 2969 5364 1678
Size: 2395 2531
Fragments arranged by size:
2531 2395 552 242 154 142 123 33
23 22
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2339 2371 2561 2593 4858
Size: 32 135 32 767 32 190 32 2265
Cuts at: 4858 4890 4932 4964 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2265 767 190 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2323 3561 3807 669
Size: 988 281 385 1238 246 3079
Fragments arranged by size:
3079 1238 988 385 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KasI G'GCGC_C
Cuts at: 2248 2965 2248
Size: 717 5500
KpnI G_GTAC'C
Cuts at: 2774 3542 2774
Size: 768 5449
MfeI C'AATT_G
Cuts at: 3816 3816
Size: 6217
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2604 5331 5515 1305
Size: 1299 2727 184 2007
Fragments arranged by size:
2727 2007 1299 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3017 3065 10
Size: 54 1425 343 1185 48 3162
Fragments arranged by size:
3162 1425 1185 343 54 48
MslI CAynn'nnrTG
Cuts at: 518 1166 2993 4175 4534 4693 518
Size: 648 1827 1182 359 159 2042
Fragments arranged by size:
2042 1827 1182 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2263 2331 2713 3228 3249 3258 3412 3460
Size: 657 68 382 515 21 9 154 48
Cuts at: 3460 3633 4310 5458 5703 1606
Size: 173 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 657 515 382 245 173
154 68 48 21 9
NaeI GCC'GGC
Cuts at: 2625 2625
Size: 6217
NarI GG'CG_CC
Cuts at: 2249 2966 2249
Size: 717 5500
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5665
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NgoMIV G'CCGG_C
Cuts at: 2623 2623
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 2044 2459 3044 3575 5120 1132
Size: 97 815 415 585 531 1545 2229
Fragments arranged by size:
2229 1545 815 585 531 415 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2010 2840 1108
Size: 902 830 4485
Fragments arranged by size:
4485 902 830
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3503 3503
Size: 6217
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6217
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspOMI G'GGCC_C
Cuts at: 3071 3071
Size: 6217
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2630 2662 2630
Size: 32 6185
PstI C_TGCA'G
Cuts at: 830 2163 3459 3569 830
Size: 1333 1296 110 3478
Fragments arranged by size:
3478 1333 1296 110
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2263 3412 3460 2263
Size: 1149 48 5020
Fragments arranged by size:
5020 1149 48
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SanDI GG'GwC_CC
Cuts at: 3503 3503
Size: 6217
SapI GCTCTTCn'nnn_
Cuts at: 2853 2853
Size: 6217
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 3309 4465 6177 3309
Size: 1156 1712 3349
Fragments arranged by size:
3349 1712 1156
SexAI A'CCwGG_T
Cuts at: 2009 2009
Size: 6217
SfcI C'TryA_G
Cuts at: 826 1045 2159 2696 3455 3565 4700 5381 5572
Size: 219 1114 537 759 110 1135 681 191
Cuts at: 5572 826
Size: 1471
Fragments arranged by size:
1471 1135 1114 759 681 537 219 191
110
SfiI GGCCn_nnn'nGGCC
Cuts at: 3390 3399 3390
Size: 9 6208
SfoI GGC'GCC
Cuts at: 2250 2967 2250
Size: 717 5500
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2633 4321 5222 5484 820
Size: 197 926 690 1688 901 262 1553
Fragments arranged by size:
1688 1553 926 901 690 262 197
SnaBI TAC'GTA
Cuts at: 493 2752 4008 493
Size: 2259 1256 2702
Fragments arranged by size:
2702 2259 1256
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 2044 2459 3044 3575 1132
Size: 912 415 585 531 3774
Fragments arranged by size:
3774 912 585 531 415
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 3453 1517
Size: 1936 4281
StyI C'CwwG_G
Cuts at: 513 1065 2152 3656 513
Size: 552 1087 1504 3074
Fragments arranged by size:
3074 1504 1087 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2567 2874 3286 3349 4244 4261 4414
Size: 1255 987 307 412 63 895 17 153
Cuts at: 4414 4599 5044 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 987 895 445 412 307 185
153 63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3102 3307 4463
Size: 274 80 33 51 1443 1125 205 1156
Cuts at: 4463 6175 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1156 1125 274 205 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3039 3071 6177 6209 3039
Size: 32 3106 32 3047
Fragments arranged by size:
3106 3047 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6217
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 4346 4346
Size: 6217
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI NruI NsiI PacI PfoI
PmlI PspXI RsrII SacI SacII SrfI SwaI