Restriction map for
pFN21AA1840
(Circular) (Six-base) MAPSORT of: pFN21AA1840.seq Check: 725 from: 1 to: 12034
pFN21AA1840 12034 bp
With 182 enzymes: SgfI *
July 14, 2018 22:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 9320 9320
Size: 12034
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9844 278
Size: 53 83 186 936 8308 2468
Fragments arranged by size:
8308 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3420 5025 5125 7978 9377 1937
Size: 1483 1605 100 2853 1399 4594
Fragments arranged by size:
4594 2853 1605 1483 1399 100
Acc65I G'GTAC_C
Cuts at: 8594 9355 8594
Size: 761 11273
AclI AA'CG_TT
Cuts at: 1563 10161 10534 1563
Size: 8598 373 3063
Fragments arranged by size:
8598 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3912 4111 4665 4724 6172 6525 6673 6729
Size: 2280 199 554 59 1448 353 148 56
Cuts at: 6729 6995 8240 8662 9218 9308 10097 11481 11749
Size: 266 1245 422 556 90 789 1384 268
Cuts at: 11749 1632
Size: 1917
Fragments arranged by size:
2280 1917 1448 1384 1245 789 556 554
422 353 268 266 199 148 90 59
56
AflII C'TTAA_G
Cuts at: 820 1017 6211 8135 8801 820
Size: 197 5194 1924 666 4053
Fragments arranged by size:
5194 4053 1924 666 197
AflIII A'CryG_T
Cuts at: 1203 2736 10933 11910 1203
Size: 1533 8197 977 1327
Fragments arranged by size:
8197 1533 1327 977
AgeI A'CCGG_T
Cuts at: 10865 10865
Size: 12034
AhdI GACnn_n'nnGTC
Cuts at: 10763 10763
Size: 12034
AleI CACnn'nnGTG
Cuts at: 1166 3258 6482 1166
Size: 2092 3224 6718
Fragments arranged by size:
6718 3224 2092
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4746 7368 1184
Size: 3562 2622 5850
Fragments arranged by size:
5850 3562 2622
AlwNI CAG_nnn'CTG
Cuts at: 3442 4452 8147 9777 11349 3442
Size: 1010 3695 1630 1572 4127
Fragments arranged by size:
4127 3695 1630 1572 1010
ApaI G_GGCC'C
Cuts at: 2252 2252
Size: 12034
ApaLI G'TGCA_C
Cuts at: 5404 10091 11247 5404
Size: 4687 1156 6191
Fragments arranged by size:
6191 4687 1156
ApoI r'AATT_y
Cuts at: 1493 2354 2637 2860 3640 4183 4326 4526 4709
Size: 861 283 223 780 543 143 200 183
Cuts at: 4709 4818 5108 5596 6333 7213 7225 9051 9101
Size: 109 290 488 737 880 12 1826 50
Cuts at: 9101 9343 9569 9721 11830 1493
Size: 242 226 152 2109 1697
Fragments arranged by size:
2109 1826 1697 880 861 780 737 543
488 290 283 242 226 223 200 183
152 143 109 50 12
AseI AT'TA_AT
Cuts at: 160 1032 3945 5663 6972 10588 160
Size: 872 2913 1718 1309 3616 1606
Fragments arranged by size:
3616 2913 1718 1606 1309 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12034
AvaI C'yCGr_G
Cuts at: 1870 1943 2133 9359 1870
Size: 73 190 7226 4545
Fragments arranged by size:
7226 4545 190 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2685 2718 2685
Size: 33 12001
BamHI G'GATC_C
Cuts at: 5338 8584 9364 9731 5338
Size: 3246 780 367 7641
Fragments arranged by size:
7641 3246 780 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2057 2095 6440 7369 7653
Size: 325 217 627 270 38 4345 929 284
Cuts at: 7653 8594 8778 9355 10810 618
Size: 941 184 577 1455 1842
Fragments arranged by size:
4345 1842 1455 941 929 627 577 325
284 270 217 184 38
BanII G_rGCy'C
Cuts at: 1391 2252 4567 5730 7464 8110 8358 9353 1391
Size: 861 2315 1163 1734 646 248 995 4072
Fragments arranged by size:
4072 2315 1734 1163 995 861 646 248
BbeI G_GCGC'C
Cuts at: 7657 7657
Size: 12034
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2739 4875 928
Size: 971 840 2136 8087
Fragments arranged by size:
8087 2136 971 840
BbvCI CC'TCA_GC
Cuts at: 9018 9018
Size: 12034
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8699 8733 10225 10259 1903
Size: 34 6762 34 1492 34 3678
Fragments arranged by size:
6762 3678 1492 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4915 6949 9926 11136 4915
Size: 2034 2977 1210 5813
Fragments arranged by size:
5813 2977 2034 1210
BclI T'GATC_A
Cuts at: 1548 3700 3709 1548
Size: 2152 9 9873
Fragments arranged by size:
9873 2152 9
BfrBI ATG'CAT
Cuts at: 3181 5528 3181
Size: 2347 9687
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2247 7277 10645
Size: 107 122 71 631 605 575 5030 3368
Cuts at: 10645 136
Size: 1525
Fragments arranged by size:
5030 3368 1525 631 605 575 122 107
71
BglII A'GATC_T
Cuts at: 2914 4278 7025 7404 7479 12029 2914
Size: 1364 2747 379 75 4550 2919
Fragments arranged by size:
4550 2919 2747 1364 379 75
BlpI GC'TnA_GC
Cuts at: 7818 9451 7818
Size: 1633 10401
Bme1580I G_kGCm'C
Cuts at: 1790 2060 2252 5408 6246 6287 7347 7374 7908
Size: 270 192 3156 838 41 1060 27 534
Cuts at: 7908 8781 10095 11251 1790
Size: 873 1314 1156 2573
Fragments arranged by size:
3156 2573 1314 1156 1060 873 838 534
270 192 41 27
BmgBI CAC'GTC
Cuts at: 1318 7366 8391 1318
Size: 6048 1025 4961
Fragments arranged by size:
6048 4961 1025
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6188 6844 8454
Size: 416 53 83 399 24 4771 656 1610
Cuts at: 8454 9765 10723 442
Size: 1311 958 1753
Fragments arranged by size:
4771 1753 1610 1311 958 656 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 5175 9329 1056
Size: 4119 4154 3761
Fragments arranged by size:
4154 4119 3761
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5694 6379 6624 6914 5694
Size: 685 245 290 10814
Fragments arranged by size:
10814 685 290 245
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2264 3086 4675 6419 6595 6743 6818 7406
Size: 1025 822 1589 1744 176 148 75 588
Cuts at: 7406 7415 7894 8661 10694 1239
Size: 9 479 767 2033 2579
Fragments arranged by size:
2579 2033 1744 1589 1025 822 767 588
479 176 148 75 9
Bpu10I CC'TnA_GC
Cuts at: 8043 9018 8043
Size: 975 11059
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2784 3480 4178 4259 5786 6575 7847 8753 10159
Size: 696 698 81 1527 789 1272 906 1406
Cuts at: 10159 11024 11322 2784
Size: 865 298 3496
Fragments arranged by size:
3496 1527 1406 1272 906 865 789 698
696 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3365 3426 10697 882
Size: 617 1866 61 7271 2219
Fragments arranged by size:
7271 2219 1866 617 61
BsaAI yAC'GTr
Cuts at: 493 1204 4310 9825 493
Size: 711 3106 5515 2702
Fragments arranged by size:
5515 3106 2702 711
BsaBI GATnn'nnATC
Cuts at: 3906 12028 3906
Size: 8122 3912
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2210 7654 9841 10223
Size: 53 83 186 936 677 5444 2187 382
Cuts at: 10223 10858 275
Size: 635 1451
Fragments arranged by size:
5444 2187 1451 936 677 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 8064 10467 10865 11139 11286 11697 8064
Size: 2403 398 274 147 411 8401
Fragments arranged by size:
8401 2403 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5232 5262 1178
Size: 30 165 30 3829 30 7950
Fragments arranged by size:
7950 3829 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2821 4175 5400 5722 6368 7328 7403 8480
Size: 1635 1354 1225 322 646 960 75 1077
Cuts at: 8480 1186
Size: 4740
Fragments arranged by size:
4740 1635 1354 1225 1077 960 646 322
75
BseYI C'CCAG_C
Cuts at: 2052 3024 3153 3570 4719 5343 6157 6859 7425
Size: 972 129 417 1149 624 814 702 566
Cuts at: 7425 8868 11237 11733 2052
Size: 1443 2369 496 2353
Fragments arranged by size:
2369 2353 1443 1149 972 814 702 624
566 496 417 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2577 3167 3297 5872 6065 7769 1163
Size: 573 841 590 130 2575 193 1704 5428
Fragments arranged by size:
5428 2575 1704 841 590 573 193 130
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9484 10245 11273 1540
Size: 275 186 7483 761 1028 2301
Fragments arranged by size:
7483 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3085 3584 5001 5408 7290 7753 8358 10095 10180
Size: 499 1417 407 1882 463 605 1737 85
Cuts at: 10180 11251 3085
Size: 1071 3868
Fragments arranged by size:
3868 1882 1737 1417 1071 605 499 463
407 85
BsmI GAATG_Cn'
Cuts at: 4883 5982 9545 9638 4883
Size: 1099 3563 93 7279
Fragments arranged by size:
7279 3563 1099 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2060 2252 3085 3584 4567 5001 5408
Size: 399 270 192 833 499 983 434 407
Cuts at: 5408 5730 6246 6287 7290 7347 7374 7464 7753
Size: 322 516 41 1003 57 27 90 289
Cuts at: 7753 7908 8110 8358 8781 9353 10095 10180 11251
Size: 155 202 248 423 572 742 85 1071
Cuts at: 11251 1391
Size: 2174
Fragments arranged by size:
2174 1071 1003 983 833 742 572 516
499 434 423 407 399 322 289 270
248 202 192 155 90 85 57 41
27
BspEI T'CCGG_A
Cuts at: 8064 11697 8064
Size: 3633 8401
BspHI T'CATG_A
Cuts at: 3519 3963 9923 11653 3519
Size: 444 5960 1730 3900
Fragments arranged by size:
5960 3900 1730 444
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 9320 9389 844
Size: 8476 69 3489
Fragments arranged by size:
8476 3489 69
BsrBI CCG'CTC
Cuts at: 1394 1823 9921 1394
Size: 429 8098 3507
Fragments arranged by size:
8098 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2414 5713 6516 8374 10529 10703 65
Size: 1183 1166 3299 803 1858 2155 174 1396
Fragments arranged by size:
3299 2155 1858 1396 1183 1166 803 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2042 7698 10678 10865 1700
Size: 110 232 5656 2980 187 2869
Fragments arranged by size:
5656 2980 2869 232 187 110
BsrGI T'GTAC_A
Cuts at: 96 4012 96
Size: 3916 8118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12034
BssSI C'ACGA_G
Cuts at: 8159 8708 10094 11106 8159
Size: 549 1386 1012 9087
Fragments arranged by size:
9087 1386 1012 549
BstAPI GCAn_nnn'nTGC
Cuts at: 794 5005 6282 6939 794
Size: 4211 1277 657 5889
Fragments arranged by size:
5889 4211 1277 657
BstBI TT'CG_AA
Cuts at: 3955 10838 3955
Size: 6883 5151
BstEII G'GTnAC_C
Cuts at: 1184 3128 1184
Size: 1944 10090
BstXI CCAn_nnnn'nTGG
Cuts at: 3544 6366 6796 7299 8014 3544
Size: 2822 430 503 715 7564
Fragments arranged by size:
7564 2822 715 503 430
BstYI r'GATC_y
Cuts at: 1972 2667 2914 4278 5149 5338 6559 7025 7404
Size: 695 247 1364 871 189 1221 466 379
Cuts at: 7404 7479 7780 7794 8584 9364 9731 10116 10133
Size: 75 301 14 790 780 367 385 17
Cuts at: 10133 11585 11671 11683 12029 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1364 1221 871 790 780 695
466 385 379 367 346 301 247 189
86 75 17 14 12
Bsu36I CC'TnA_GG
Cuts at: 2934 7759 7853 2934
Size: 4825 94 7115
Fragments arranged by size:
7115 4825 94
BtgI C'CryG_G
Cuts at: 513 1065 2004 2061 2067 3086 3526 7960 8329
Size: 552 939 57 6 1019 440 4434 369
Cuts at: 8329 513
Size: 4218
Fragments arranged by size:
4434 4218 1019 939 552 440 369 57
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2129 487
Size: 683 56 903 10392
Fragments arranged by size:
10392 903 683 56
BtsI GCAGTG_nn'
Cuts at: 2607 2823 3309 3392 6396 6845 7741 9550 10344
Size: 216 486 83 3004 449 896 1809 794
Cuts at: 10344 10364 2607
Size: 20 4277
Fragments arranged by size:
4277 3004 1809 896 794 486 449 216
83 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 12034
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11999
DraI TTT'AAA
Cuts at: 3510 4095 4203 4332 9338 3510
Size: 585 108 129 5006 6206
Fragments arranged by size:
6206 5006 585 129 108
DraIII CAC_nnn'GTG
Cuts at: 3408 8271 3408
Size: 4863 7171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 11041 809
Size: 507 9725 1802
Fragments arranged by size:
9725 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2985 4833
Size: 54 1425 50 275 18 234 921 1848
Cuts at: 4833 5624 5826 7068 8926 9481 10370 11999 8
Size: 791 202 1242 1858 555 889 1629 43
Fragments arranged by size:
1858 1848 1629 1425 1242 921 889 791
555 275 234 202 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9481 1537
Size: 275 7669 4090
Fragments arranged by size:
7669 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3495 3647 3741 5399 6728 6905 8163 9135
Size: 2142 152 94 1658 1329 177 1258 972
Cuts at: 9135 9964 1353
Size: 829 3423
Fragments arranged by size:
3423 2142 1658 1329 1258 972 829 177
152 94
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2029 10621 10995 11141 387
Size: 1064 138 440 8592 374 146 1280
Fragments arranged by size:
8592 1280 1064 440 374 146 138
EcoICRI GAG'CTC
Cuts at: 8356 8356
Size: 12034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2264 3086 3912 4111 4665 4675 4724
Size: 393 632 822 826 199 554 10 49
Cuts at: 4724 6172 6419 6525 6595 6673 6729 6743 6818
Size: 1448 247 106 70 78 56 14 75
Cuts at: 6818 6995 7406 7415 7894 8240 8661 8662 9218
Size: 177 411 9 479 346 421 1 556
Cuts at: 9218 9308 10097 10694 11481 11749 1239
Size: 90 789 597 787 268 1524
Fragments arranged by size:
1524 1448 826 822 789 787 632 597
556 554 479 421 411 393 346 268
247 199 177 106 90 78 75 70
56 49 14 10 9 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2925 5751 9300 2925
Size: 2826 3549 5659
Fragments arranged by size:
5659 3549 2826
EcoO109I rG'GnC_Cy
Cuts at: 2248 2249 2930 6403 7984 8131 8806 9292 2248
Size: 1 681 3473 1581 147 675 486 4990
Fragments arranged by size:
4990 3473 1581 681 675 486 147 1
EcoRI G'AATT_C
Cuts at: 2637 9343 9721 2637
Size: 6706 378 4950
Fragments arranged by size:
6706 4950 378
EcoRV GAT'ATC
Cuts at: 2443 2443
Size: 12034
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3013 4300 5966 6099 6237 6734 8572 9089 3013
Size: 1287 1666 133 138 497 1838 517 5958
Fragments arranged by size:
5958 1838 1666 1287 517 497 138 133
FspI TGC'GCA
Cuts at: 2147 10540 2147
Size: 8393 3641
HaeII r_GCGC'y
Cuts at: 1678 4089 7321 7657 11181 1678
Size: 2411 3232 336 3524 2531
Fragments arranged by size:
3524 3232 2531 2411 336
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3211 3243 3507 3539 3604
Size: 32 135 32 1639 32 264 32 65
Cuts at: 3604 3636 3701 3733 4123 4155 5232 5264 6155
Size: 32 65 32 390 32 1077 32 891
Cuts at: 6155 6187 7616 7648 7786 7818 7971 8003 8731
Size: 32 1429 32 138 32 153 32 728
Cuts at: 8731 8763 10675 10707 10749 10781 1373
Size: 32 1912 32 42 32 2626
Fragments arranged by size:
2626 1912 1639 1429 1077 891 728 390
264 153 138 135 65 65 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2962 5126 7979 9378 9624 669
Size: 988 281 1024 2164 2853 1399 246 3079
Fragments arranged by size:
3079 2853 2164 1399 1024 988 281 246
HindIII A'AGCT_T
Cuts at: 748 4397 6824 6866 6926 748
Size: 3649 2427 42 60 5856
Fragments arranged by size:
5856 3649 2427 60 42
HpaI GTT'AAC
Cuts at: 2962 9624 2962
Size: 6662 5372
KasI G'GCGC_C
Cuts at: 7653 7653
Size: 12034
KpnI G_GTAC'C
Cuts at: 8598 9359 8598
Size: 761 11273
MfeI C'AATT_G
Cuts at: 3830 4268 9633 3830
Size: 438 5365 6231
Fragments arranged by size:
6231 5365 438
MluI A'CGCG_T
Cuts at: 11910 11910
Size: 12034
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2859 8286 8562 8752 8802 11148 11332 1305
Size: 1554 5427 276 190 50 2346 184 2007
Fragments arranged by size:
5427 2346 2007 1554 276 190 184 50
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2987 4835 5828 7070 8928
Size: 54 1425 343 1155 1848 993 1242 1858
Cuts at: 8928 10
Size: 3116
Fragments arranged by size:
3116 1858 1848 1425 1242 1155 993 343
54
MslI CAynn'nnrTG
Cuts at: 518 1166 3258 3531 3564 4020 4728 6482 9185
Size: 648 2092 273 33 456 708 1754 2703
Cuts at: 9185 9992 10351 10510 518
Size: 807 359 159 2042
Fragments arranged by size:
2703 2092 2042 1754 807 708 648 456
359 273 159 33
MspA1I CmG'CkG
Cuts at: 1606 2063 2143 3442 3628 5689 8260 8717 8785
Size: 457 80 1299 186 2061 2571 457 68
Cuts at: 8785 8881 9450 10127 11275 11520 1606
Size: 96 569 677 1148 245 2120
Fragments arranged by size:
2571 2120 2061 1299 1148 677 569 457
457 245 186 96 80 68
NaeI GCC'GGC
Cuts at: 2044 2044
Size: 12034
NarI GG'CG_CC
Cuts at: 7654 7654
Size: 12034
NcoI C'CATG_G
Cuts at: 513 1065 2004 2067 3086 3526 7960 8329 513
Size: 552 939 63 1019 440 4434 369 4218
Fragments arranged by size:
4434 4218 1019 939 552 440 369 63
NdeI CA'TA_TG
Cuts at: 387 2698 387
Size: 2311 9723
NgoMIV G'CCGG_C
Cuts at: 2042 2042
Size: 12034
NheI G'CTAG_C
Cuts at: 1052 5171 9325 1052
Size: 4119 4154 3761
Fragments arranged by size:
4154 4119 3761
NotI GC'GGCC_GC
Cuts at: 9481 9481
Size: 12034
NruI TCG'CGA
Cuts at: 2027 2027
Size: 12034
NsiI A_TGCA'T
Cuts at: 3183 5530 3183
Size: 2347 9687
NspI r_CATG'y
Cuts at: 1132 1229 2740 3385 3599 5766 7284 7602 8437
Size: 97 1511 645 214 2167 1518 318 835
Cuts at: 8437 8686 9325 9392 10937 1132
Size: 249 639 67 1545 2229
Fragments arranged by size:
2229 2167 1545 1518 1511 835 645 639
318 249 214 97 67
PasI CC'CwG_GG
Cuts at: 1348 3376 8754 1348
Size: 2028 5378 4628
Fragments arranged by size:
5378 4628 2028
PciI A'CATG_T
Cuts at: 2736 10933 2736
Size: 8197 3837
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4452 1108
Size: 3344 8690
PfoI T'CCnGG_A
Cuts at: 3093 3229 5655 8638 3093
Size: 136 2426 2983 6489
Fragments arranged by size:
6489 2983 2426 136
PmeI GTTT'AAAC
Cuts at: 9338 9338
Size: 12034
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6221 6253 10103 10135 11616 11648 6221
Size: 32 3850 32 1481 32 6607
Fragments arranged by size:
6607 3850 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2930 7984 9292 2930
Size: 5054 1308 5672
Fragments arranged by size:
5672 5054 1308
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 12034
PsiI TTA'TAA
Cuts at: 6699 7716 9604 6699
Size: 1017 1888 9129
Fragments arranged by size:
9129 1888 1017
PspOMI G'GGCC_C
Cuts at: 2248 2248
Size: 12034
PstI C_TGCA'G
Cuts at: 830 2014 2633 5010 6056 6123 7047 7748 8907
Size: 1184 619 2377 1046 67 924 701 1159
Cuts at: 8907 9386 830
Size: 479 3478
Fragments arranged by size:
3478 2377 1184 1159 1046 924 701 619
479 67
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12034
PvuII CAG'CTG
Cuts at: 2143 3442 3628 5689 8260 8717 8785 2143
Size: 1299 186 2061 2571 457 68 5392
Fragments arranged by size:
5392 2571 2061 1299 457 186 68
SacI G_AGCT'C
Cuts at: 8358 8358
Size: 12034
SacII CC_GC'GG
Cuts at: 2064 2064
Size: 12034
SalI G'TCGA_C
Cuts at: 1936 5124 7977 9376 1936
Size: 3188 2853 1399 4594
Fragments arranged by size:
4594 3188 2853 1399
SapI GCTCTTCn'nnn_
Cuts at: 3647 3741 6728 3647
Size: 94 2987 8953
Fragments arranged by size:
8953 2987 94
SbfI CC_TGCA'GG
Cuts at: 9386 9386
Size: 12034
ScaI AGT'ACT
Cuts at: 2565 6185 10282 11994 2565
Size: 3620 4097 1712 2605
Fragments arranged by size:
4097 3620 2605 1712
SexAI A'CCwGG_T
Cuts at: 5562 5562
Size: 12034
SfcI C'TryA_G
Cuts at: 826 1045 2010 2401 2629 3200 3417 3996 4371
Size: 219 965 391 228 571 217 579 375
Cuts at: 4371 5006 5517 5580 5892 6052 6119 6833 6994
Size: 635 511 63 312 160 67 714 161
Cuts at: 6994 7043 7744 8420 8903 9382 10517 11198 11389
Size: 49 701 676 483 479 1135 681 191
Cuts at: 11389 826
Size: 1471
Fragments arranged by size:
1471 1135 965 714 701 681 676 635
579 571 511 483 479 391 375 312
228 219 217 191 161 160 67 63
49
SfoI GGC'GCC
Cuts at: 7655 7655
Size: 12034
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12034
SgrAI Cr'CCGG_yG
Cuts at: 1700 2042 1700
Size: 342 11692
SmaI CCC'GGG
Cuts at: 1872 2135 9361 1872
Size: 263 7226 4545
Fragments arranged by size:
7226 4545 263
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2799 3495 4157 4274 5801 6211
Size: 197 926 856 696 662 117 1527 410
Cuts at: 6211 6554 7826 8135 8768 8801 10138 11039 11301
Size: 343 1272 309 633 33 1337 901 262
Cuts at: 11301 820
Size: 1553
Fragments arranged by size:
1553 1527 1337 1272 926 901 856 696
662 633 410 343 309 262 197 117
33
SnaBI TAC'GTA
Cuts at: 493 9825 493
Size: 9332 2702
SpeI A'CTAG_T
Cuts at: 152 7487 152
Size: 7335 4699
SphI G_CATG'C
Cuts at: 1132 3385 9392 1132
Size: 2253 6007 3774
Fragments arranged by size:
6007 3774 2253
SspI AAT'ATT
Cuts at: 5 52 9958 5
Size: 47 9906 2081
Fragments arranged by size:
9906 2081 47
StuI AGG'CCT
Cuts at: 1517 4179 8114 9023 1517
Size: 2662 3935 909 4528
Fragments arranged by size:
4528 3935 2662 909
StyI C'CwwG_G
Cuts at: 513 1065 2004 2067 3086 3397 3526 3977 5099
Size: 552 939 63 1019 311 129 451 1122
Cuts at: 5099 7335 7374 7731 7960 8007 8329 8844 9473
Size: 2236 39 357 229 47 322 515 629
Cuts at: 9473 513
Size: 3074
Fragments arranged by size:
3074 2236 1122 1019 939 629 552 515
451 357 322 311 229 129 63 47
39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2895 6171 6335 7305 7439 7803 8109
Size: 1255 1315 3276 164 970 134 364 306
Cuts at: 8109 10061 10078 10231 10416 10861 325
Size: 1952 17 153 185 445 1498
Fragments arranged by size:
3276 1952 1498 1315 1255 970 445 364
306 185 164 153 134 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2563 3137 4012
Size: 274 80 33 51 1443 586 574 875
Cuts at: 4012 5871 6183 10280 11992 96
Size: 1859 312 4097 1712 138
Fragments arranged by size:
4097 1859 1712 1443 875 586 574 312
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3704 3736 3837 3869 6794 6826 11994 12026 3704
Size: 32 101 32 2925 32 5168 32 3712
Fragments arranged by size:
5168 3712 2925 101 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 9182 1316
Size: 7866 4168
XbaI T'CTAG_A
Cuts at: 9370 9370
Size: 12034
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3237 6253 7433 7918 3237
Size: 3016 1180 485 7353
Fragments arranged by size:
7353 3016 1180 485
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12034
XmaI C'CCGG_G
Cuts at: 1870 2133 9359 1870
Size: 263 7226 4545
Fragments arranged by size:
7226 4545 263
XmnI GAAnn'nnTTC
Cuts at: 2916 10163 2916
Size: 7247 4787
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9842 276
Size: 53 83 186 936 8308 2468
Fragments arranged by size:
8308 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BsiWI BsmBI BstZ17I FseI
FspAI PacI PmlI PspXI PsrI RsrII SanDI SfiI
SrfI SwaI