Restriction map for
pFN21AA1838
(Circular) (Six-base) MAPSORT of: pFN21AA1838.seq Check: 5920 from: 1 to: 7435
pFN21AA1838 7435 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3941 3941
Size: 7435
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5245 278
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2162 3107 3287 3323 3359 3395 3431 3891
Size: 225 945 180 36 36 36 36 460
Cuts at: 3891 4778 1937
Size: 887 4594
Fragments arranged by size:
4594 945 887 460 225 180 36 36
36 36
Acc65I G'GTAC_C
Cuts at: 4756 4756
Size: 7435
AclI AA'CG_TT
Cuts at: 1563 5562 5935 1563
Size: 3999 373 3063
Fragments arranged by size:
3999 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3282 3711 4544 5498 6882 7150 1632
Size: 1650 429 833 954 1384 268 1917
Fragments arranged by size:
1917 1650 1384 954 833 429 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7238
AflIII A'CryG_T
Cuts at: 1203 3176 6334 7311 1203
Size: 1973 3158 977 1327
Fragments arranged by size:
3158 1973 1327 977
AgeI A'CCGG_T
Cuts at: 6266 6266
Size: 7435
AhdI GACnn_n'nnGTC
Cuts at: 3887 6164 3887
Size: 2277 5158
AleI CACnn'nnGTG
Cuts at: 1166 3680 1166
Size: 2514 4921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4552 1184
Size: 3368 4067
AlwNI CAG_nnn'CTG
Cuts at: 3044 3080 3116 3152 3188 3224 3260 3296 3332
Size: 36 36 36 36 36 36 36 36
Cuts at: 3332 3368 3404 3440 3476 3725 3933 5178 6750
Size: 36 36 36 36 249 208 1245 1572
Cuts at: 6750 3044
Size: 3729
Fragments arranged by size:
3729 1572 1245 249 208 36 36 36
36 36 36 36 36 36 36 36
36
ApaLI G'TGCA_C
Cuts at: 5492 6648 5492
Size: 1156 6279
ApoI r'AATT_y
Cuts at: 1493 2068 3620 4511 4744 4970 5122 7231 1493
Size: 575 1552 891 233 226 152 2109 1697
Fragments arranged by size:
2109 1697 1552 891 575 233 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5989 160
Size: 872 4957 1606
Fragments arranged by size:
4957 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
AvaI C'yCGr_G
Cuts at: 1870 1943 4760 1870
Size: 73 2817 4545
Fragments arranged by size:
4545 2817 73
BamHI G'GATC_C
Cuts at: 4765 5132 4765
Size: 367 7068
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3981 4756 6211 618
Size: 325 217 627 2194 775 1455 1842
Fragments arranged by size:
2194 1842 1455 775 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2592 4325 4582 4592 4625 4646 4754 1391
Size: 1201 1733 257 10 33 21 108 4072
Fragments arranged by size:
4072 1733 1201 257 108 33 21 10
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2544 3567 3675 4394 4621 928
Size: 971 645 1023 108 719 227 3742
Fragments arranged by size:
3742 1023 971 719 645 227 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5626 5660 1903
Size: 34 3689 34 3678
Fragments arranged by size:
3689 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2115 3972 5327 6537 2115
Size: 1857 1355 1210 3013
Fragments arranged by size:
3013 1857 1355 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7435
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2691 6046 136
Size: 107 122 71 631 605 1019 3355 1525
Fragments arranged by size:
3355 1525 1019 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4496 7430 4496
Size: 2934 4501
BlpI GC'TnA_GC
Cuts at: 4852 4852
Size: 7435
Bme1580I G_kGCm'C
Cuts at: 1790 5496 6652 1790
Size: 3706 1156 2573
Fragments arranged by size:
3706 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 3757 1318
Size: 2439 4996
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2456 3914 4594
Size: 416 53 83 399 24 1039 1458 680
Cuts at: 4594 5166 6124 442
Size: 572 958 1753
Fragments arranged by size:
1753 1458 1039 958 680 572 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2417 1056
Size: 1361 6074
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2651 2651
Size: 7435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2151 2492 4232 4609 6095 1239
Size: 912 341 1740 377 1486 2579
Fragments arranged by size:
2579 1740 1486 912 377 341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 4105 5560 6425 6723 2306
Size: 1799 1455 865 298 3018
Fragments arranged by size:
3018 1799 1455 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4677 6098 882
Size: 617 3178 1421 2219
Fragments arranged by size:
3178 2219 1421 617
BsaAI yAC'GTr
Cuts at: 493 1204 3177 5226 493
Size: 711 1973 2049 2702
Fragments arranged by size:
2702 2049 1973 711
BsaBI GATnn'nnATC
Cuts at: 7429 7429
Size: 7435
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3006 5242 5624 6259
Size: 53 83 186 936 1473 2236 382 635
Cuts at: 6259 275
Size: 1451
Fragments arranged by size:
2236 1473 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5868 6266 6540 6687 7098 5868
Size: 398 274 147 411 6205
Fragments arranged by size:
6205 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7210
Fragments arranged by size:
7210 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4108 1186
Size: 2922 4513
BseYI C'CCAG_C
Cuts at: 2246 3851 4318 6638 7134 2246
Size: 1605 467 2320 496 2547
Fragments arranged by size:
2547 2320 1605 496 467
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4334 1163
Size: 573 2598 4264
Fragments arranged by size:
4264 2598 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4885 5646 6674 1540
Size: 275 186 2884 761 1028 2301
Fragments arranged by size:
2884 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 2592 5496 5581 6652 2091
Size: 501 2904 85 1071 2874
Fragments arranged by size:
2904 2874 1071 501 85
BsmI GAATG_Cn'
Cuts at: 3815 4946 5039 3815
Size: 1131 93 6211
Fragments arranged by size:
6211 1131 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2592 4325 4582 4592 4625 4646
Size: 399 301 501 1733 257 10 33 21
Cuts at: 4646 4754 5496 5581 6652 1391
Size: 108 742 85 1071 2174
Fragments arranged by size:
2174 1733 1071 742 501 399 301 257
108 85 33 21 10
BspEI T'CCGG_A
Cuts at: 7098 7098
Size: 7435
BspHI T'CATG_A
Cuts at: 3789 5324 7054 3789
Size: 1535 1730 4170
Fragments arranged by size:
4170 1730 1535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2051 3941 4790 844
Size: 1207 1890 849 3489
Fragments arranged by size:
3489 1890 1207 849
BsrBI CCG'CTC
Cuts at: 1394 1823 5322 1394
Size: 429 3499 3507
Fragments arranged by size:
3507 3499 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5930 6104 65
Size: 1183 4682 174 1396
Fragments arranged by size:
4682 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6079 6266 1700
Size: 110 4269 187 2869
Fragments arranged by size:
4269 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2057 3071 3179 3251 3500 3536 3572 3644
Size: 1961 1014 108 72 249 36 36 72
Cuts at: 3644 3780 96
Size: 136 3751
Fragments arranged by size:
3751 1961 1014 249 136 108 72 72
36 36
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7435
BssSI C'ACGA_G
Cuts at: 2995 5495 6507 2995
Size: 2500 1012 3923
Fragments arranged by size:
3923 2500 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4545 794
Size: 3751 3684
BstBI TT'CG_AA
Cuts at: 6239 6239
Size: 7435
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7435
BstXI CCAn_nnnn'nTGG
Cuts at: 2175 3682 2175
Size: 1507 5928
BstYI r'GATC_y
Cuts at: 1972 2178 3541 4496 4765 5132 5517 5534 6986
Size: 206 1363 955 269 367 385 17 1452
Cuts at: 6986 7072 7084 7430 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1363 955 385 367 346 269
206 86 17 12
BstZ17I GTA'TAC
Cuts at: 2163 3108 3288 3324 3360 3396 3432 2163
Size: 945 180 36 36 36 36 6166
Fragments arranged by size:
6166 945 180 36 36 36 36
Bsu36I CC'TnA_GG
Cuts at: 2407 2407
Size: 7435
BtgI C'CryG_G
Cuts at: 513 1065 2004 2939 3711 4336 4676 513
Size: 552 939 935 772 625 340 3272
Fragments arranged by size:
3272 939 935 772 625 552 340
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6696
Fragments arranged by size:
6696 683 56
BtsI GCAGTG_nn'
Cuts at: 3802 4951 5745 5765 3802
Size: 1149 794 20 5472
Fragments arranged by size:
5472 1149 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7435
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7400
DraI TTT'AAA
Cuts at: 4739 4739
Size: 7435
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6442 809
Size: 507 5126 1802
Fragments arranged by size:
5126 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2188 2661 4573
Size: 54 1425 50 275 18 358 473 1912
Cuts at: 4573 4882 5771 7400 8
Size: 309 889 1629 43
Fragments arranged by size:
1912 1629 1425 889 473 358 309 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4882 1537
Size: 275 3070 4090
Fragments arranged by size:
4090 3070 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2679 2853 4122 5365 1353
Size: 1326 174 1269 1243 3423
Fragments arranged by size:
3423 1326 1269 1243 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6022 6396 6542 387
Size: 1064 138 4433 374 146 1280
Fragments arranged by size:
4433 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2590 2590
Size: 7435
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2492 3282 3711 4232 4544 4609
Size: 393 519 341 790 429 521 312 65
Cuts at: 4609 5498 6095 6882 7150 1239
Size: 889 597 787 268 1524
Fragments arranged by size:
1524 889 790 787 597 521 519 429
393 341 312 268 65
EcoO109I rG'GnC_Cy
Cuts at: 3077 3185 3257 3401 3437 3686 3077
Size: 108 72 144 36 249 6826
Fragments arranged by size:
6826 249 144 108 72 36
EcoRI G'AATT_C
Cuts at: 4744 5122 4744
Size: 378 7057
EcoRV GAT'ATC
Cuts at: 2340 2340
Size: 7435
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3851 3851
Size: 7435
FspI TGC'GCA
Cuts at: 4236 5941 4236
Size: 1705 5730
HaeII r_GCGC'y
Cuts at: 1678 4084 6582 1678
Size: 2406 2498 2531
Fragments arranged by size:
2531 2498 2406
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2801 2833 2854 2886 2996
Size: 32 135 32 1229 32 21 32 110
Cuts at: 2996 3028 3032 3064 3464 3496 3533 3565 3873
Size: 32 4 32 400 32 37 32 308
Cuts at: 3873 3905 4488 4520 6076 6108 6150 6182 1373
Size: 32 583 32 1556 32 42 32 2626
Fragments arranged by size:
2626 1556 1229 583 400 308 135 110
42 37 32 32 32 32 32 32
32 32 32 32 32 32 21 4
HincII GTy'rAC
Cuts at: 669 1657 1938 4363 4779 5025 669
Size: 988 281 2425 416 246 3079
Fragments arranged by size:
3079 2425 988 416 281 246
HindIII A'AGCT_T
Cuts at: 748 2725 4158 4427 748
Size: 1977 1433 269 3756
Fragments arranged by size:
3756 1977 1433 269
HpaI GTT'AAC
Cuts at: 5025 5025
Size: 7435
KpnI G_GTAC'C
Cuts at: 4760 4760
Size: 7435
MfeI C'AATT_G
Cuts at: 5034 5034
Size: 7435
MluI A'CGCG_T
Cuts at: 7311 7311
Size: 7435
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6549 6733 1305
Size: 5244 184 2007
Fragments arranged by size:
5244 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2190 10
Size: 54 1425 343 358 5255
Fragments arranged by size:
5255 1425 358 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2053 2615 3680 5393 5752 5911
Size: 648 843 44 562 1065 1713 359 159
Cuts at: 5911 518
Size: 2042
Fragments arranged by size:
2042 1713 1065 843 648 562 359 159
44
MspA1I CmG'CkG
Cuts at: 1606 2246 3886 4003 4318 4338 4851 5528 6676
Size: 640 1640 117 315 20 513 677 1148
Cuts at: 6676 6921 1606
Size: 245 2120
Fragments arranged by size:
2120 1640 1148 677 640 513 315 245
117 20
NcoI C'CATG_G
Cuts at: 513 1065 2004 2939 3711 513
Size: 552 939 935 772 4237
Fragments arranged by size:
4237 939 935 772 552
NdeI CA'TA_TG
Cuts at: 387 3533 387
Size: 3146 4289
NheI G'CTAG_C
Cuts at: 1052 2413 1052
Size: 1361 6074
NotI GC'GGCC_GC
Cuts at: 4882 4882
Size: 7435
NspI r_CATG'y
Cuts at: 1132 1229 2719 4226 4374 4793 6338 1132
Size: 97 1490 1507 148 419 1545 2229
Fragments arranged by size:
2229 1545 1507 1490 419 148 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7435
PciI A'CATG_T
Cuts at: 6334 6334
Size: 7435
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7435
PfoI T'CCnGG_A
Cuts at: 2107 2342 2107
Size: 235 7200
PmeI GTTT'AAAC
Cuts at: 4739 4739
Size: 7435
PmlI CAC'GTG
Cuts at: 3177 3177
Size: 7435
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5504 5536 7017 7049 5504
Size: 32 1481 32 5890
Fragments arranged by size:
5890 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2565 1613
Size: 952 6483
PsiI TTA'TAA
Cuts at: 2898 5005 2898
Size: 2107 5328
PstI C_TGCA'G
Cuts at: 830 2627 4008 4137 4787 830
Size: 1797 1381 129 650 3478
Fragments arranged by size:
3478 1797 1381 650 129
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7435
PvuII CAG'CTG
Cuts at: 2246 4318 2246
Size: 2072 5363
SacI G_AGCT'C
Cuts at: 2592 2592
Size: 7435
SacII CC_GC'GG
Cuts at: 4339 4339
Size: 7435
SalI G'TCGA_C
Cuts at: 1936 4777 1936
Size: 2841 4594
SapI GCTCTTCn'nnn_
Cuts at: 4122 4122
Size: 7435
SbfI CC_TGCA'GG
Cuts at: 4787 4787
Size: 7435
ScaI AGT'ACT
Cuts at: 4445 5683 7395 4445
Size: 1238 1712 4485
Fragments arranged by size:
4485 1712 1238
SexAI A'CCwGG_T
Cuts at: 3774 3774
Size: 7435
SfcI C'TryA_G
Cuts at: 826 1045 2623 3603 4004 4133 4283 4783 5918
Size: 219 1578 980 401 129 150 500 1135
Cuts at: 5918 6599 6790 826
Size: 681 191 1471
Fragments arranged by size:
1578 1471 1135 980 681 500 401 219
191 150 129
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7435
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7435
SmaI CCC'GGG
Cuts at: 1872 4762 1872
Size: 2890 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 4120 5539 6440 6702 820
Size: 197 926 378 1799 1419 901 262 1553
Fragments arranged by size:
1799 1553 1419 926 901 378 262 197
SnaBI TAC'GTA
Cuts at: 493 5226 493
Size: 4733 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7435
SphI G_CATG'C
Cuts at: 1132 4226 4793 1132
Size: 3094 567 3774
Fragments arranged by size:
3774 3094 567
SspI AAT'ATT
Cuts at: 5 52 4044 5359 5
Size: 47 3992 1315 2081
Fragments arranged by size:
3992 2081 1315 47
StuI AGG'CCT
Cuts at: 1517 3845 3860 4241 4544 1517
Size: 2328 15 381 303 4408
Fragments arranged by size:
4408 2328 381 303 15
StyI C'CwwG_G
Cuts at: 513 1065 2004 2689 2939 3711 4252 4874 513
Size: 552 939 685 250 772 541 622 3074
Fragments arranged by size:
3074 939 772 685 622 552 541 250
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2296 5462 5479 5632 5817 6262
Size: 1255 417 299 3166 17 153 185 445
Cuts at: 6262 325
Size: 1498
Fragments arranged by size:
3166 1498 1255 445 417 299 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2057 3071 3179
Size: 274 80 33 51 1443 80 1014 108
Cuts at: 3179 3251 3500 3536 3572 3644 3780 4443 5681
Size: 72 249 36 36 72 136 663 1238
Cuts at: 5681 7393 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1238 1014 663 274 249 138
136 108 80 80 72 72 51 36
36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2170 2202 2447 2479 3741 3773 4378 4410 7395
Size: 32 245 32 1262 32 605 32 2985
Cuts at: 7395 7427 2170
Size: 32 2178
Fragments arranged by size:
2985 2178 1262 605 245 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2612 3992 1316
Size: 1296 1380 4759
Fragments arranged by size:
4759 1380 1296
XbaI T'CTAG_A
Cuts at: 4771 4771
Size: 7435
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7435
XmaI C'CCGG_G
Cuts at: 1870 4760 1870
Size: 2890 4545
XmnI GAAnn'nnTTC
Cuts at: 3024 5564 3024
Size: 2540 4895
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5243 276
Size: 53 83 186 936 3709 2468
Fragments arranged by size:
3709 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI DraIII EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI
PspOMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI
SwaI XcmI