Restriction map for
pFN21AA1824
(Circular) (Six-base) MAPSORT of: pFN21AA1824.seq Check: 8067 from: 1 to: 7531
pFN21AA1824 7531 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2939 2939
Size: 7531
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5341 278
Size: 53 83 186 936 3805 2468
Fragments arranged by size:
3805 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4874 1937
Size: 2937 4594
Acc65I G'GTAC_C
Cuts at: 4283 4852 4283
Size: 569 6962
AclI AA'CG_TT
Cuts at: 1563 5658 6031 1563
Size: 4095 373 3063
Fragments arranged by size:
4095 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4200 5594 6978 7246 1632
Size: 2568 1394 1384 268 1917
Fragments arranged by size:
2568 1917 1394 1384 268
AfeI AGC'GCT
Cuts at: 3362 3362
Size: 7531
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7334
AflIII A'CryG_T
Cuts at: 1203 2415 2919 3509 6430 7407 1203
Size: 1212 504 590 2921 977 1327
Fragments arranged by size:
2921 1327 1212 977 590 504
AgeI A'CCGG_T
Cuts at: 6362 6362
Size: 7531
AhdI GACnn_n'nnGTC
Cuts at: 3382 6260 3382
Size: 2878 4653
AleI CACnn'nnGTG
Cuts at: 1166 2348 3508 1166
Size: 1182 1160 5189
Fragments arranged by size:
5189 1182 1160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3774 1184
Size: 2590 4941
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2247 2279 2247
Size: 32 7499
AlwNI CAG_nnn'CTG
Cuts at: 2209 2749 3541 4171 4604 5274 6846 2209
Size: 540 792 630 433 670 1572 2894
Fragments arranged by size:
2894 1572 792 670 630 540 433
ApaI G_GGCC'C
Cuts at: 3459 3459
Size: 7531
ApaLI G'TGCA_C
Cuts at: 5588 6744 5588
Size: 1156 6375
ApoI r'AATT_y
Cuts at: 1493 2183 2928 3981 4801 4840 5066 5218 7327
Size: 690 745 1053 820 39 226 152 2109
Cuts at: 7327 1493
Size: 1697
Fragments arranged by size:
2109 1697 1053 820 745 690 226 152
39
AseI AT'TA_AT
Cuts at: 160 1032 6085 160
Size: 872 5053 1606
Fragments arranged by size:
5053 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7531
AvaI C'yCGr_G
Cuts at: 1870 1943 3786 4856 1870
Size: 73 1843 1070 4545
Fragments arranged by size:
4545 1843 1070 73
BamHI G'GATC_C
Cuts at: 4861 5228 4861
Size: 367 7164
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3768 4273 4283 4852 6307
Size: 325 217 627 1981 505 10 569 1455
Cuts at: 6307 618
Size: 1842
Fragments arranged by size:
1981 1842 1455 627 569 505 325 217
10
BanII G_rGCy'C
Cuts at: 1391 2459 2544 2604 2900 3089 3299 3459 4850
Size: 1068 85 60 296 189 210 160 1391
Cuts at: 4850 1391
Size: 4072
Fragments arranged by size:
4072 1391 1068 296 210 189 160 85
60
BbeI G_GCGC'C
Cuts at: 3772 3772
Size: 7531
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3243 3378 3785 3798 3846 928
Size: 971 1344 135 407 13 48 4613
Fragments arranged by size:
4613 1344 971 407 135 48 13
BbvCI CC'TCA_GC
Cuts at: 4371 4371
Size: 7531
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5722 5756 1903
Size: 34 3785 34 3678
Fragments arranged by size:
3785 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5423 6633 5423
Size: 1210 6321
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7531
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2028 2121 2163
Size: 107 122 71 631 605 356 93 42
Cuts at: 2163 4755 6142 136
Size: 2592 1387 1525
Fragments arranged by size:
2592 1525 1387 631 605 356 122 107
93 71 42
BglII A'GATC_T
Cuts at: 3078 3637 7526 3078
Size: 559 3889 3083
Fragments arranged by size:
3889 3083 559
BlpI GC'TnA_GC
Cuts at: 4948 4948
Size: 7531
Bme1580I G_kGCm'C
Cuts at: 1790 3459 3808 5592 6748 1790
Size: 1669 349 1784 1156 2573
Fragments arranged by size:
2573 1784 1669 1156 349
BmgBI CAC'GTC
Cuts at: 1318 2416 1318
Size: 1098 6433
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2453 3795 4071
Size: 416 53 83 399 24 1036 1342 276
Cuts at: 4071 4444 5262 6220 442
Size: 373 818 958 1753
Fragments arranged by size:
1753 1342 1036 958 818 416 399 373
276 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7531
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 4303 4822 2660
Size: 1643 519 5369
Fragments arranged by size:
5369 1643 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2254 2379 3103 3132 3313 4586 6191 1239
Size: 1015 125 724 29 181 1273 1605 2579
Fragments arranged by size:
2579 1605 1273 1015 724 181 125 29
Bpu10I CC'TnA_GC
Cuts at: 3364 3903 4358 4371 3364
Size: 539 455 13 6524
Fragments arranged by size:
6524 539 455 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5656 6521 6819 5656
Size: 865 298 6368
Fragments arranged by size:
6368 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2218 3108 4040 4670 6194 882
Size: 617 719 890 932 630 1524 2219
Fragments arranged by size:
2219 1524 932 890 719 630 617
BsaAI yAC'GTr
Cuts at: 493 1204 3510 5322 493
Size: 711 2306 1812 2702
Fragments arranged by size:
2702 2306 1812 711
BsaBI GATnn'nnATC
Cuts at: 7525 7525
Size: 7531
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2113 2764 3769 5338
Size: 53 83 186 936 580 651 1005 1569
Cuts at: 5338 5720 6355 275
Size: 382 635 1451
Fragments arranged by size:
1569 1451 1005 936 651 635 580 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 5964 6362 6636 6783 7194 5964
Size: 398 274 147 411 6301
Fragments arranged by size:
6301 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2591 2621 3866 3896 1178
Size: 30 165 30 1188 30 1245 30 4813
Fragments arranged by size:
4813 1245 1188 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2026 2122 2141 2235 3620 3731 3734 3871
Size: 840 96 19 94 1385 111 3 137
Cuts at: 3871 3874 4377 4388 4481 4486 4798 1186
Size: 3 503 11 93 5 312 3919
Fragments arranged by size:
3919 1385 840 503 312 137 111 96
94 93 19 11 5 3 3
BseYI C'CCAG_C
Cuts at: 2061 2742 3347 3662 4108 4226 4717 6734 7230
Size: 681 605 315 446 118 491 2017 496
Cuts at: 7230 2061
Size: 2362
Fragments arranged by size:
2362 2017 681 605 496 491 446 315
118
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3326 1163
Size: 573 1590 5368
Fragments arranged by size:
5368 1590 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3127 4981 5742 6770 1540
Size: 275 186 1126 1854 761 1028 2301
Fragments arranged by size:
2301 1854 1126 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2304 2781 3299 3914 4251 4323 5592 5677 6748
Size: 477 518 615 337 72 1269 85 1071
Cuts at: 6748 2304
Size: 3087
Fragments arranged by size:
3087 1269 1071 615 518 477 337 85
72
BsmI GAATG_Cn'
Cuts at: 3763 5042 5135 3763
Size: 1279 93 6159
Fragments arranged by size:
6159 1279 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2104 2104
Size: 7531
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2304 2459 2544 2604 2781 2900 3089
Size: 399 514 155 85 60 177 119 189
Cuts at: 3089 3299 3459 3808 3914 4251 4323 4850 5592
Size: 210 160 349 106 337 72 527 742
Cuts at: 5592 5677 6748 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 527 514 399 349 337
210 189 177 160 155 119 106 85
85 72 60
BspEI T'CCGG_A
Cuts at: 7194 7194
Size: 7531
BspHI T'CATG_A
Cuts at: 2425 5420 7150 2425
Size: 2995 1730 2806
Fragments arranged by size:
2995 2806 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2939 4179 4886 844
Size: 2095 1240 707 3489
Fragments arranged by size:
3489 2095 1240 707
BsrBI CCG'CTC
Cuts at: 1394 1823 3907 5418 1394
Size: 429 2084 1511 3507
Fragments arranged by size:
3507 2084 1511 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2371 3395 4155 4237 6026 6200 65
Size: 1183 1123 1024 760 82 1789 174 1396
Fragments arranged by size:
1789 1396 1183 1123 1024 760 174 82
BsrFI r'CCGG_y
Cuts at: 1700 1810 6175 6362 1700
Size: 110 4365 187 2869
Fragments arranged by size:
4365 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2916 96
Size: 2820 4711
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7531
BssSI C'ACGA_G
Cuts at: 5591 6603 5591
Size: 1012 6519
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7531
BstBI TT'CG_AA
Cuts at: 2656 6335 2656
Size: 3679 3852
BstEII G'GTnAC_C
Cuts at: 1184 3466 3657 1184
Size: 2282 191 5058
Fragments arranged by size:
5058 2282 191
BstXI CCAn_nnnn'nTGG
Cuts at: 2611 4505 2611
Size: 1894 5637
BstYI r'GATC_y
Cuts at: 1972 2938 3078 3637 4861 5228 5613 5630 7082
Size: 966 140 559 1224 367 385 17 1452
Cuts at: 7082 7168 7180 7526 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1224 966 559 385 367 346
140 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2756 2756
Size: 7531
BtgI C'CryG_G
Cuts at: 513 1065 2533 3653 513
Size: 552 1468 1120 4391
Fragments arranged by size:
4391 1468 1120 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6792
Fragments arranged by size:
6792 683 56
BtsI GCAGTG_nn'
Cuts at: 2407 5047 5841 5861 2407
Size: 2640 794 20 4077
Fragments arranged by size:
4077 2640 794 20
ClaI AT'CG_AT
Cuts at: 1554 3001 1554
Size: 1447 6084
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7496
DraI TTT'AAA
Cuts at: 4204 4835 4204
Size: 631 6900
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6538 809
Size: 507 5222 1802
Fragments arranged by size:
5222 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2155 2497 2613
Size: 54 1425 50 275 18 325 342 116
Cuts at: 2613 3650 4747 4978 5867 7496 8
Size: 1037 1097 231 889 1629 43
Fragments arranged by size:
1629 1425 1097 1037 889 342 325 275
231 116 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4978 1537
Size: 275 3166 4090
Fragments arranged by size:
4090 3166 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2003 3058 3159 4203 4588 5461 1353
Size: 650 1055 101 1044 385 873 3423
Fragments arranged by size:
3423 1055 1044 873 650 385 101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6118 6492 6638 387
Size: 1064 138 4529 374 146 1280
Fragments arranged by size:
4529 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3297 3297
Size: 7531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2254 2379 3103 3132 3313 4200 4586
Size: 393 622 125 724 29 181 887 386
Cuts at: 4586 5594 6191 6978 7246 1239
Size: 1008 597 787 268 1524
Fragments arranged by size:
1524 1008 887 787 724 622 597 393
386 268 181 125 29
EcoO109I rG'GnC_Cy
Cuts at: 2080 2146 2739 3455 3456 4168 4354 4446 4702
Size: 66 593 716 1 712 186 92 256
Cuts at: 4702 2080
Size: 4909
Fragments arranged by size:
4909 716 712 593 256 186 92 66
1
EcoRI G'AATT_C
Cuts at: 2183 2928 4801 4840 5218 2183
Size: 745 1873 39 378 4496
Fragments arranged by size:
4496 1873 745 378 39
FspI TGC'GCA
Cuts at: 6037 6037
Size: 7531
HaeII r_GCGC'y
Cuts at: 1678 3364 3559 3772 6678 1678
Size: 1686 195 213 2906 2531
Fragments arranged by size:
2906 2531 1686 213 195
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3368 3400 3509 3541 3863
Size: 32 135 32 1796 32 109 32 322
Cuts at: 3863 3866 3895 3898 3950 3982 4604 4636 6172
Size: 3 29 3 52 32 622 32 1536
Cuts at: 6172 6204 6246 6278 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1796 1536 622 322 135 109 52
42 32 32 32 32 32 32 32
32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2971 3013 4875 5121 669
Size: 988 281 1033 42 1862 246 3079
Fragments arranged by size:
3079 1862 1033 988 281 246 42
HindIII A'AGCT_T
Cuts at: 748 3990 748
Size: 3242 4289
HpaI GTT'AAC
Cuts at: 3013 5121 3013
Size: 2108 5423
KasI G'GCGC_C
Cuts at: 3768 3768
Size: 7531
KpnI G_GTAC'C
Cuts at: 4287 4856 4287
Size: 569 6962
MfeI C'AATT_G
Cuts at: 5130 5130
Size: 7531
MluI A'CGCG_T
Cuts at: 7407 7407
Size: 7531
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 2334 3107 3361 6645 6829 1305
Size: 909 120 773 254 3284 184 2007
Fragments arranged by size:
3284 2007 909 773 254 184 120
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2615 4749 10
Size: 54 1425 343 783 2134 2792
Fragments arranged by size:
2792 2134 1425 783 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2430 3041 3508 4148 4503 5489
Size: 648 1182 82 611 467 640 355 986
Cuts at: 5489 5848 6007 518
Size: 359 159 2042
Fragments arranged by size:
2042 1182 986 648 640 611 467 359
355 159 82
MspA1I CmG'CkG
Cuts at: 1606 2065 2290 3147 3754 4425 4947 5624 6772
Size: 459 225 857 607 671 522 677 1148
Cuts at: 6772 7017 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 857 677 671 607 522 459
245 225
NarI GG'CG_CC
Cuts at: 3769 3769
Size: 7531
NcoI C'CATG_G
Cuts at: 513 1065 2533 513
Size: 552 1468 5511
Fragments arranged by size:
5511 1468 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7531
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7531
NotI GC'GGCC_GC
Cuts at: 4978 4978
Size: 7531
NspI r_CATG'y
Cuts at: 1132 1229 2923 4889 6434 1132
Size: 97 1694 1966 1545 2229
Fragments arranged by size:
2229 1966 1694 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7531
PciI A'CATG_T
Cuts at: 2919 6430 2919
Size: 3511 4020
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2209 2749 1108
Size: 1101 540 5890
Fragments arranged by size:
5890 1101 540
PfoI T'CCnGG_A
Cuts at: 3231 3231
Size: 7531
PmeI GTTT'AAAC
Cuts at: 4835 4835
Size: 7531
PmlI CAC'GTG
Cuts at: 3510 3510
Size: 7531
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5600 5632 7113 7145 5600
Size: 32 1481 32 5986
Fragments arranged by size:
5986 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2080 2146 2739 4168 4446 4702 2080
Size: 66 593 1429 278 256 4909
Fragments arranged by size:
4909 1429 593 278 256 66
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7531
PsiI TTA'TAA
Cuts at: 3987 5101 3987
Size: 1114 6417
PspOMI G'GGCC_C
Cuts at: 3455 3455
Size: 7531
PstI C_TGCA'G
Cuts at: 830 2129 2938 4190 4883 830
Size: 1299 809 1252 693 3478
Fragments arranged by size:
3478 1299 1252 809 693
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7531
PvuII CAG'CTG
Cuts at: 2290 2290
Size: 7531
SacI G_AGCT'C
Cuts at: 3299 3299
Size: 7531
SalI G'TCGA_C
Cuts at: 1936 4873 1936
Size: 2937 4594
SanDI GG'GwC_CC
Cuts at: 2080 2739 2080
Size: 659 6872
SapI GCTCTTCn'nnn_
Cuts at: 3058 3058
Size: 7531
SbfI CC_TGCA'GG
Cuts at: 4883 4883
Size: 7531
ScaI AGT'ACT
Cuts at: 3328 5779 7491 3328
Size: 2451 1712 3368
Fragments arranged by size:
3368 2451 1712
SfcI C'TryA_G
Cuts at: 826 1045 2125 2934 4186 4314 4879 6014 6695
Size: 219 1080 809 1252 128 565 1135 681
Cuts at: 6695 6886 826
Size: 191 1471
Fragments arranged by size:
1471 1252 1135 1080 809 681 565 219
191 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 4755 4755
Size: 7531
SfoI GGC'GCC
Cuts at: 3770 3770
Size: 7531
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7531
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7531
SmaI CCC'GGG
Cuts at: 1872 4858 1872
Size: 2986 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5635 6536 6798 820
Size: 197 926 3692 901 262 1553
Fragments arranged by size:
3692 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5322 493
Size: 4829 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7531
SphI G_CATG'C
Cuts at: 1132 4889 1132
Size: 3757 3774
SspI AAT'ATT
Cuts at: 5 52 5455 5
Size: 47 5403 2081
Fragments arranged by size:
5403 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7531
StyI C'CwwG_G
Cuts at: 513 1065 2533 4053 4398 4970 513
Size: 552 1468 1520 345 572 3074
Fragments arranged by size:
3074 1520 1468 572 552 345
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3217 3520 3676 5558 5575 5728 5913
Size: 1255 1637 303 156 1882 17 153 185
Cuts at: 5913 6358 325
Size: 445 1498
Fragments arranged by size:
1882 1637 1498 1255 445 303 185 156
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2916 3107 3168
Size: 274 80 33 51 1443 939 191 61
Cuts at: 3168 3326 5777 7489 96
Size: 158 2451 1712 138
Fragments arranged by size:
2451 1712 1443 939 274 191 158 138
80 61 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7491 7523 7491
Size: 32 7499
Tth111I GACn'n_nGTC
Cuts at: 1316 4450 1316
Size: 3134 4397
XbaI T'CTAG_A
Cuts at: 4867 4867
Size: 7531
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3344 3344
Size: 7531
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7531
XmaI C'CCGG_G
Cuts at: 1870 4856 1870
Size: 2986 4545
XmnI GAAnn'nnTTC
Cuts at: 5660 5660
Size: 7531
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5339 276
Size: 53 83 186 936 3805 2468
Fragments arranged by size:
3805 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoNI
EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspXI PsrI RsrII SacII SexAI SrfI SwaI