Restriction map for
pFN21AA1816
(Circular) (Six-base) MAPSORT of: pFN21AA1816.seq Check: 4132 from: 1 to: 8104
pFN21AA1816 8104 bp
With 182 enzymes: SgfI *
February 27, 2009 14:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5914 278
Size: 53 83 186 936 4378 2468
Fragments arranged by size:
4378 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5447 1937
Size: 3510 4594
Acc65I G'GTAC_C
Cuts at: 2286 5425 2286
Size: 3139 4965
AclI AA'CG_TT
Cuts at: 1563 6231 6604 1563
Size: 4668 373 3063
Fragments arranged by size:
4668 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2628 6167 7551 7819 1632
Size: 996 3539 1384 268 1917
Fragments arranged by size:
3539 1917 1384 996 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7907
AflIII A'CryG_T
Cuts at: 1203 3106 3387 5262 7003 7980 1203
Size: 1903 281 1875 1741 977 1327
Fragments arranged by size:
1903 1875 1741 1327 977 281
AgeI A'CCGG_T
Cuts at: 6935 6935
Size: 8104
AhdI GACnn_n'nnGTC
Cuts at: 6833 6833
Size: 8104
AleI CACnn'nnGTG
Cuts at: 1166 2222 1166
Size: 1056 7048
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2556 1184
Size: 1372 6732
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2695 2727 2695
Size: 32 8072
AlwNI CAG_nnn'CTG
Cuts at: 2986 3041 3365 4267 5005 5847 7419 2986
Size: 55 324 902 738 842 1572 3671
Fragments arranged by size:
3671 1572 902 842 738 324 55
ApaI G_GGCC'C
Cuts at: 2183 4237 2183
Size: 2054 6050
ApaLI G'TGCA_C
Cuts at: 6161 7317 6161
Size: 1156 6948
ApoI r'AATT_y
Cuts at: 1493 2554 2577 3023 5413 5639 5791 7900 1493
Size: 1061 23 446 2390 226 152 2109 1697
Fragments arranged by size:
2390 2109 1697 1061 446 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 2910 2939 6658 160
Size: 872 1878 29 3719 1606
Fragments arranged by size:
3719 1878 1606 872 29
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8104
AvaI C'yCGr_G
Cuts at: 1870 1943 2407 5092 5429 1870
Size: 73 464 2685 337 4545
Fragments arranged by size:
4545 2685 464 337 73
AvrII C'CTAG_G
Cuts at: 4811 4811
Size: 8104
BamHI G'GATC_C
Cuts at: 5434 5801 5434
Size: 367 7737
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 2286 2381 2387 4943
Size: 325 217 627 475 24 95 6 2556
Cuts at: 4943 5105 5425 6880 618
Size: 162 320 1455 1842
Fragments arranged by size:
2556 1842 1455 627 475 325 320 217
162 95 24 6
BanII G_rGCy'C
Cuts at: 1391 2183 2509 4237 5423 1391
Size: 792 326 1728 1186 4072
Fragments arranged by size:
4072 1728 1186 792 326
BbeI G_GCGC'C
Cuts at: 2385 4947 2385
Size: 2562 5542
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3003 3686 3993 928
Size: 971 1104 683 307 5039
Fragments arranged by size:
5039 1104 971 683 307
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6295 6329 1903
Size: 34 4358 34 3678
Fragments arranged by size:
4358 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5996 7206 5996
Size: 1210 6894
BclI T'GATC_A
Cuts at: 1548 2466 2920 3723 4225 1548
Size: 918 454 803 502 5427
Fragments arranged by size:
5427 918 803 502 454
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2187 3971 6715
Size: 107 122 71 631 605 515 1784 2744
Cuts at: 6715 136
Size: 1525
Fragments arranged by size:
2744 1784 1525 631 605 515 122 107
71
BglII A'GATC_T
Cuts at: 2728 8099 2728
Size: 5371 2733
BlpI GC'TnA_GC
Cuts at: 5521 5521
Size: 8104
Bme1580I G_kGCm'C
Cuts at: 1790 2183 3378 3976 4128 4237 5039 6165 7321
Size: 393 1195 598 152 109 802 1126 1156
Cuts at: 7321 1790
Size: 2573
Fragments arranged by size:
2573 1195 1156 1126 802 598 393 152
109
BmgBI CAC'GTC
Cuts at: 1318 2269 5263 1318
Size: 951 2994 4159
Fragments arranged by size:
4159 2994 951
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2619 4231 4373
Size: 416 53 83 399 24 1202 1612 142
Cuts at: 4373 5196 5835 6793 442
Size: 823 639 958 1753
Fragments arranged by size:
1753 1612 1202 958 823 639 416 399
142 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8104
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 8104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2333 2339 2378 2810 3267 3378 3944 4340
Size: 1094 6 39 432 457 111 566 396
Cuts at: 4340 4433 6764 1239
Size: 93 2331 2579
Fragments arranged by size:
2579 2331 1094 566 457 432 396 111
93 39 6
Bpu10I CC'TnA_GC
Cuts at: 2298 3980 4135 5141 2298
Size: 1682 155 1006 5261
Fragments arranged by size:
5261 1682 1006 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 6229 7094 7392 6229
Size: 865 298 6941
Fragments arranged by size:
6941 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 3038 3552 4153 6767 882
Size: 617 1021 518 514 601 2614 2219
Fragments arranged by size:
2614 2219 1021 617 601 518 514
BsaAI yAC'GTr
Cuts at: 493 1204 5895 493
Size: 711 4691 2702
Fragments arranged by size:
4691 2702 711
BsaBI GATnn'nnATC
Cuts at: 8098 8098
Size: 8104
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2382 4944 5911 6293
Size: 53 83 186 936 849 2562 967 382
Cuts at: 6293 6928 275
Size: 635 1451
Fragments arranged by size:
2562 1451 967 936 849 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6537 6935 7209 7356 7767 6537
Size: 398 274 147 411 6874
Fragments arranged by size:
6874 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2695 2725 4900 4930 1178
Size: 30 165 30 1292 30 2175 30 4352
Fragments arranged by size:
4352 2175 1292 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2643 4900 1186
Size: 1457 2257 4390
Fragments arranged by size:
4390 2257 1457
BseYI C'CCAG_C
Cuts at: 2418 3377 4767 4964 5056 5182 5291 7307 7803
Size: 959 1390 197 92 126 109 2016 496
Cuts at: 7803 2418
Size: 2719
Fragments arranged by size:
2719 2016 1390 959 496 197 126 109
92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3405 4035 4403 5343 1163
Size: 573 1669 630 368 940 3924
Fragments arranged by size:
3924 1669 940 630 573 368
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2202 5554 6315 7343 1540
Size: 275 186 201 3352 761 1028 2301
Fragments arranged by size:
3352 2301 1028 761 275 201 186
BsiHKAI G_wGCw'C
Cuts at: 2509 4616 4870 6165 6250 7321 2509
Size: 2107 254 1295 85 1071 3292
Fragments arranged by size:
3292 2107 1295 1071 254 85
BsmI GAATG_Cn'
Cuts at: 5254 5615 5708 5254
Size: 361 93 7650
Fragments arranged by size:
7650 361 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2183 2509 3378 3976 4128 4237 4616
Size: 399 393 326 869 598 152 109 379
Cuts at: 4616 4870 5039 5423 6165 6250 7321 1391
Size: 254 169 384 742 85 1071 2174
Fragments arranged by size:
2174 1071 869 742 598 399 393 384
379 326 254 169 152 109 85
BspEI T'CCGG_A
Cuts at: 7767 7767
Size: 8104
BspHI T'CATG_A
Cuts at: 4008 4206 5993 7723 4008
Size: 198 1787 1730 4389
Fragments arranged by size:
4389 1787 1730 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3681 4378 4705 5459 844
Size: 2837 697 327 754 3489
Fragments arranged by size:
3489 2837 754 697 327
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 5245 5991 1394
Size: 429 407 3015 746 3507
Fragments arranged by size:
3507 3015 746 429 407
BsrDI GCAATG_nn'
Cuts at: 65 1248 6599 6773 65
Size: 1183 5351 174 1396
Fragments arranged by size:
5351 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2156 2384 2390 3347 6748 6935 1700
Size: 110 346 228 6 957 3401 187 2869
Fragments arranged by size:
3401 2869 957 346 228 187 110 6
BsrGI T'GTAC_A
Cuts at: 96 4602 96
Size: 4506 3598
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8104
BssSI C'ACGA_G
Cuts at: 6164 7176 6164
Size: 1012 7092
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3242 4900 5080 794
Size: 2448 1658 180 3818
Fragments arranged by size:
3818 2448 1658 180
BstBI TT'CG_AA
Cuts at: 2581 6908 2581
Size: 4327 3777
BstEII G'GTnAC_C
Cuts at: 1184 5396 1184
Size: 4212 3892
BstXI CCAn_nnnn'nTGG
Cuts at: 2537 4520 2537
Size: 1983 6121
BstYI r'GATC_y
Cuts at: 1972 2728 3119 5434 5801 6186 6203 7655 7741
Size: 756 391 2315 367 385 17 1452 86
Cuts at: 7741 7753 8099 1972
Size: 12 346 1977
Fragments arranged by size:
2315 1977 1452 756 391 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2967 3553 5256 2967
Size: 586 1703 5815
Fragments arranged by size:
5815 1703 586
BtgI C'CryG_G
Cuts at: 513 1065 2004 2220 3114 3171 4038 4063 5064
Size: 552 939 216 894 57 867 25 1001
Cuts at: 5064 513
Size: 3553
Fragments arranged by size:
3553 1001 939 894 867 552 216 57
25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 5070 487
Size: 683 56 3844 3521
Fragments arranged by size:
3844 3521 683 56
BtsI GCAGTG_nn'
Cuts at: 2636 2788 3342 5620 6414 6434 2636
Size: 152 554 2278 794 20 4306
Fragments arranged by size:
4306 2278 794 554 152 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5260 5295 529
Size: 35 4696 35 3338
Fragments arranged by size:
4696 3338 35 35
DraI TTT'AAA
Cuts at: 5408 5408
Size: 8104
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7111 809
Size: 507 5795 1802
Fragments arranged by size:
5795 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2199 2952 3174
Size: 54 1425 50 275 18 369 753 222
Cuts at: 3174 3345 3852 4530 5551 6440 8069 8
Size: 171 507 678 1021 889 1629 43
Fragments arranged by size:
1629 1425 1021 889 753 678 507 369
275 222 171 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2199 5551 1537
Size: 275 387 3352 4090
Fragments arranged by size:
4090 3352 387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2661 3000 3071 4962 6034 1353
Size: 1308 339 71 1891 1072 3423
Fragments arranged by size:
3423 1891 1308 1072 339 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2225 2459 4328 6691 7065 7211
Size: 1064 138 636 234 1869 2363 374 146
Cuts at: 7211 387
Size: 1280
Fragments arranged by size:
2363 1869 1280 1064 636 374 234 146
138
EcoICRI GAG'CTC
Cuts at: 2507 2507
Size: 8104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2333 2339 2378 2628 2810 3267 3378
Size: 393 701 6 39 250 182 457 111
Cuts at: 3378 3944 4340 4433 6167 6764 7551 7819 1239
Size: 566 396 93 1734 597 787 268 1524
Fragments arranged by size:
1734 1524 787 701 597 566 457 396
393 268 250 182 111 93 39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 4810 5144 4810
Size: 334 7770
EcoO109I rG'GnC_Cy
Cuts at: 2179 2180 2983 3362 3557 3964 4106 4169 4505
Size: 1 803 379 195 407 142 63 336
Cuts at: 4505 4815 5046 2179
Size: 310 231 5237
Fragments arranged by size:
5237 803 407 379 336 310 231 195
142 63 1
EcoRI G'AATT_C
Cuts at: 2577 3023 5413 5791 2577
Size: 446 2390 378 4890
Fragments arranged by size:
4890 2390 446 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5008 5008
Size: 8104
FspI TGC'GCA
Cuts at: 4545 6610 4545
Size: 2065 6039
HaeII r_GCGC'y
Cuts at: 1678 2326 2385 4947 7251 1678
Size: 648 59 2562 2304 2531
Fragments arranged by size:
2562 2531 2304 648 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2128 2160 6745 6777 6819
Size: 32 135 32 556 32 4585 32 42
Cuts at: 6819 6851 1373
Size: 32 2626
Fragments arranged by size:
4585 2626 556 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2272 2773 5448 5694 669
Size: 988 281 334 501 2675 246 3079
Fragments arranged by size:
3079 2675 988 501 334 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8104
HpaI GTT'AAC
Cuts at: 5694 5694
Size: 8104
KasI G'GCGC_C
Cuts at: 2381 4943 2381
Size: 2562 5542
KpnI G_GTAC'C
Cuts at: 2290 5429 2290
Size: 3139 4965
MfeI C'AATT_G
Cuts at: 5703 5703
Size: 8104
MluI A'CGCG_T
Cuts at: 7980 7980
Size: 8104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2478 2588 4066 4530 4696 7218 7402 1305
Size: 1173 110 1478 464 166 2522 184 2007
Fragments arranged by size:
2522 2007 1478 1173 464 184 166 110
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2954 3854 4532 10
Size: 54 1425 343 1122 900 678 3582
Fragments arranged by size:
3582 1425 1122 900 678 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2222 3719 3816 4619 4748 6062 6421
Size: 648 1056 1497 97 803 129 1314 359
Cuts at: 6421 6580 518
Size: 159 2042
Fragments arranged by size:
2042 1497 1314 1056 803 648 359 159
129 97
MspA1I CmG'CkG
Cuts at: 1606 2027 2129 2311 2350 4231 4366 4541 5060
Size: 421 102 182 39 1881 135 175 519
Cuts at: 5060 5520 6197 7345 7590 1606
Size: 460 677 1148 245 2120
Fragments arranged by size:
2120 1881 1148 677 519 460 421 245
182 175 135 102 39
NaeI GCC'GGC
Cuts at: 2386 2386
Size: 8104
NarI GG'CG_CC
Cuts at: 2382 4944 2382
Size: 2562 5542
NcoI C'CATG_G
Cuts at: 513 1065 2004 3114 4038 5064 513
Size: 552 939 1110 924 1026 3553
Fragments arranged by size:
3553 1110 1026 939 924 552
NdeI CA'TA_TG
Cuts at: 387 3574 387
Size: 3187 4917
NgoMIV G'CCGG_C
Cuts at: 2384 2384
Size: 8104
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8104
NotI GC'GGCC_GC
Cuts at: 5551 5551
Size: 8104
NspI r_CATG'y
Cuts at: 1132 1229 2635 3110 3391 4459 4747 4903 5164
Size: 97 1406 475 281 1068 288 156 261
Cuts at: 5164 5462 7007 1132
Size: 298 1545 2229
Fragments arranged by size:
2229 1545 1406 1068 475 298 288 281
261 156 97
PasI CC'CwG_GG
Cuts at: 1348 3969 4520 4681 1348
Size: 2621 551 161 4771
Fragments arranged by size:
4771 2621 551 161
PciI A'CATG_T
Cuts at: 3106 3387 7003 3106
Size: 281 3616 4207
Fragments arranged by size:
4207 3616 281
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2522 1108
Size: 1414 6690
PfoI T'CCnGG_A
Cuts at: 5313 5313
Size: 8104
PmeI GTTT'AAAC
Cuts at: 5408 5408
Size: 8104
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2695 2727 6173 6205 7686 7718 2695
Size: 32 3446 32 1481 32 3081
Fragments arranged by size:
3446 3081 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2983 3557 4505 5046 2983
Size: 574 948 541 6041
Fragments arranged by size:
6041 948 574 541
PshAI GACnn'nnGTC
Cuts at: 1613 3828 1613
Size: 2215 5889
PsiI TTA'TAA
Cuts at: 5674 5674
Size: 8104
PspOMI G'GGCC_C
Cuts at: 2179 4233 2179
Size: 2054 6050
PstI C_TGCA'G
Cuts at: 830 3595 3678 4839 5456 830
Size: 2765 83 1161 617 3478
Fragments arranged by size:
3478 2765 1161 617 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8104
PvuII CAG'CTG
Cuts at: 2311 4541 5060 2311
Size: 2230 519 5355
Fragments arranged by size:
5355 2230 519
SacI G_AGCT'C
Cuts at: 2509 2509
Size: 8104
SalI G'TCGA_C
Cuts at: 1936 5446 1936
Size: 3510 4594
SapI GCTCTTCn'nnn_
Cuts at: 3071 3071
Size: 8104
SbfI CC_TGCA'GG
Cuts at: 5456 5456
Size: 8104
ScaI AGT'ACT
Cuts at: 2122 6352 8064 2122
Size: 4230 1712 2162
Fragments arranged by size:
4230 2162 1712
SfcI C'TryA_G
Cuts at: 826 1045 3591 3648 3674 4835 5452 6587 7268
Size: 219 2546 57 26 1161 617 1135 681
Cuts at: 7268 7459 826
Size: 191 1471
Fragments arranged by size:
2546 1471 1161 1135 681 617 219 191
57 26
SfoI GGC'GCC
Cuts at: 2383 4945 2383
Size: 2562 5542
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8104
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8104
SmaI CCC'GGG
Cuts at: 1872 5094 5431 1872
Size: 3222 337 4545
Fragments arranged by size:
4545 3222 337
SmlI C'TyrA_G
Cuts at: 820 1017 1943 6208 7109 7371 820
Size: 197 926 4265 901 262 1553
Fragments arranged by size:
4265 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5895 493
Size: 5402 2702
SpeI A'CTAG_T
Cuts at: 152 3179 4913 152
Size: 3027 1734 3343
Fragments arranged by size:
3343 3027 1734
SphI G_CATG'C
Cuts at: 1132 4459 4747 4903 5164 5462 1132
Size: 3327 288 156 261 298 3774
Fragments arranged by size:
3774 3327 298 288 261 156
SspI AAT'ATT
Cuts at: 5 52 3778 6028 5
Size: 47 3726 2250 2081
Fragments arranged by size:
3726 2250 2081 47
StuI AGG'CCT
Cuts at: 1517 3136 4805 1517
Size: 1619 1669 4816
Fragments arranged by size:
4816 1669 1619
StyI C'CwwG_G
Cuts at: 513 1065 2004 3114 4038 4811 4829 5064 5543
Size: 552 939 1110 924 773 18 235 479
Cuts at: 5543 513
Size: 3074
Fragments arranged by size:
3074 1110 939 924 773 552 479 235
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4702 5196 6131 6148 6301 6486 6931
Size: 1255 3122 494 935 17 153 185 445
Cuts at: 6931 325
Size: 1498
Fragments arranged by size:
3122 1498 1255 935 494 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2120 4602 6350
Size: 274 80 33 51 1443 143 2482 1748
Cuts at: 6350 8062 96
Size: 1712 138
Fragments arranged by size:
2482 1748 1712 1443 274 143 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4900 4932 8064 8096 4900
Size: 32 3132 32 4908
Fragments arranged by size:
4908 3132 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8104
XbaI T'CTAG_A
Cuts at: 2722 5440 2722
Size: 2718 5386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 5364 5364
Size: 8104
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8104
XmaI C'CCGG_G
Cuts at: 1870 5092 5429 1870
Size: 3222 337 4545
Fragments arranged by size:
4545 3222 337
XmnI GAAnn'nnTTC
Cuts at: 6233 6233
Size: 8104
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5912 276
Size: 53 83 186 936 4378 2468
Fragments arranged by size:
4378 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbvCI BfrBI BsiWI BsmBI
BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI
SrfI SwaI