Restriction map for
pFN21AA1795
(Circular) (Six-base) MAPSORT of: pFN21AA1795.seq Check: 9002 from: 1 to: 6004
pFN21AA1795 6004 bp
With 182 enzymes: SgfI *
February 27, 2009 14:32 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3814 278
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2960 3347 1937
Size: 1023 387 4594
Fragments arranged by size:
4594 1023 387
Acc65I G'GTAC_C
Cuts at: 2392 3325 2392
Size: 933 5071
AclI AA'CG_TT
Cuts at: 1563 4131 4504 1563
Size: 2568 373 3063
Fragments arranged by size:
3063 2568 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4067 5451 5719 1632
Size: 2435 1384 268 1917
Fragments arranged by size:
2435 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5807
AflIII A'CryG_T
Cuts at: 1203 4903 5880 1203
Size: 3700 977 1327
Fragments arranged by size:
3700 1327 977
AgeI A'CCGG_T
Cuts at: 4835 4835
Size: 6004
AhdI GACnn_n'nnGTC
Cuts at: 4733 4733
Size: 6004
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6004
AlwNI CAG_nnn'CTG
Cuts at: 2951 3747 5319 2951
Size: 796 1572 3636
Fragments arranged by size:
3636 1572 796
ApaLI G'TGCA_C
Cuts at: 2643 4061 5217 2643
Size: 1418 1156 3430
Fragments arranged by size:
3430 1418 1156
ApoI r'AATT_y
Cuts at: 1493 3313 3539 3691 5800 1493
Size: 1820 226 152 2109 1697
Fragments arranged by size:
2109 1820 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3212 4558 160
Size: 872 2180 1346 1606
Fragments arranged by size:
2180 1606 1346 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
AvaI C'yCGr_G
Cuts at: 1870 1943 3329 1870
Size: 73 1386 4545
Fragments arranged by size:
4545 1386 73
BamHI G'GATC_C
Cuts at: 3334 3701 3334
Size: 367 5637
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2392 3325 4780 618
Size: 325 217 627 605 933 1455 1842
Fragments arranged by size:
1842 1455 933 627 605 325 217
BanII G_rGCy'C
Cuts at: 1391 2945 3323 1391
Size: 1554 378 4072
Fragments arranged by size:
4072 1554 378
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5033
BbvCI CC'TCA_GC
Cuts at: 2615 2615
Size: 6004
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4195 4229 1903
Size: 34 50 34 2174 34 3678
Fragments arranged by size:
3678 2174 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3896 5106 3896
Size: 1210 4794
BclI T'GATC_A
Cuts at: 1548 2815 1548
Size: 1267 4737
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2588 4615 136
Size: 107 122 71 631 605 916 2027 1525
Fragments arranged by size:
2027 1525 916 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5999 5999
Size: 6004
BlpI GC'TnA_GC
Cuts at: 2162 2952 3421 2162
Size: 790 469 4745
Fragments arranged by size:
4745 790 469
Bme1580I G_kGCm'C
Cuts at: 1790 2647 3179 4065 5221 1790
Size: 857 532 886 1156 2573
Fragments arranged by size:
2573 1156 886 857 532
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6004
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3735 4693 442
Size: 416 53 83 399 24 2318 958 1753
Fragments arranged by size:
2318 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6004
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2322 2322
Size: 6004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2292 2302 3086 3170 4664 1239
Size: 861 192 10 784 84 1494 2579
Fragments arranged by size:
2579 1494 861 784 192 84 10
Bpu10I CC'TnA_GC
Cuts at: 2297 2615 2297
Size: 318 5686
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2310 4129 4994 5292 2310
Size: 1819 865 298 3022
Fragments arranged by size:
3022 1819 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2341 2994 4667 882
Size: 617 842 653 1673 2219
Fragments arranged by size:
2219 1673 842 653 617
BsaAI yAC'GTr
Cuts at: 493 1204 3795 493
Size: 711 2591 2702
Fragments arranged by size:
2702 2591 711
BsaBI GATnn'nnATC
Cuts at: 5998 5998
Size: 6004
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3811 4193 4828 275
Size: 53 83 186 936 2278 382 635 1451
Fragments arranged by size:
2278 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4437 4835 5109 5256 5667 4437
Size: 398 274 147 411 4774
Fragments arranged by size:
4774 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2113 2143 2993 3023 1178
Size: 30 165 30 710 30 850 30 4159
Fragments arranged by size:
4159 850 710 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6004
BseYI C'CCAG_C
Cuts at: 2069 2351 3172 5207 5703 2069
Size: 282 821 2035 496 2370
Fragments arranged by size:
2370 2035 821 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5431
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3454 4215 5243 1540
Size: 275 186 1453 761 1028 2301
Fragments arranged by size:
2301 1453 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2163 2647 4065 4150 5221 2163
Size: 484 1418 85 1071 2946
Fragments arranged by size:
2946 1418 1071 484 85
BsmI GAATG_Cn'
Cuts at: 3515 3608 3515
Size: 93 5911
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2163 2647 2945 3179 3323 4065 4150
Size: 399 373 484 298 234 144 742 85
Cuts at: 4150 5221 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 484 399 373 298 234
144 85
BspEI T'CCGG_A
Cuts at: 5667 5667
Size: 6004
BspHI T'CATG_A
Cuts at: 3893 5623 3893
Size: 1730 4274
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3359 844
Size: 2515 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3891 1394
Size: 429 2068 3507
Fragments arranged by size:
3507 2068 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4499 4673 65
Size: 1183 3251 174 1396
Fragments arranged by size:
3251 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4648 4835 1700
Size: 110 2838 187 2869
Fragments arranged by size:
2869 2838 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6004
BssHII G'CGCG_C
Cuts at: 1925 2862 1925
Size: 937 5067
BssSI C'ACGA_G
Cuts at: 4064 5076 4064
Size: 1012 4992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3281 794
Size: 2487 3517
BstBI TT'CG_AA
Cuts at: 4808 4808
Size: 6004
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6004
BstYI r'GATC_y
Cuts at: 1972 2788 3256 3334 3701 4086 4103 5555 5641
Size: 816 468 78 367 385 17 1452 86
Cuts at: 5641 5653 5999 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 816 468 385 367 346 86
78 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2133 513
Size: 552 1068 4384
Fragments arranged by size:
4384 1068 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5265
Fragments arranged by size:
5265 683 56
BtsI GCAGTG_nn'
Cuts at: 3520 4314 4334 3520
Size: 794 20 5190
Fragments arranged by size:
5190 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6004
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3063 3098 529
Size: 35 2499 35 3435
Fragments arranged by size:
3435 2499 35 35
DraI TTT'AAA
Cuts at: 2763 3308 2763
Size: 545 5459
DraIII CAC_nnn'GTG
Cuts at: 3281 3281
Size: 6004
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2207 5011 809
Size: 507 891 2804 1802
Fragments arranged by size:
2804 1802 891 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3451 4340 5969
Size: 54 1425 50 275 18 1621 889 1629
Cuts at: 5969 8
Size: 43
Fragments arranged by size:
1629 1621 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3451 1537
Size: 275 1639 4090
Fragments arranged by size:
4090 1639 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3934 1353
Size: 2581 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4591 4965 5111 387
Size: 1064 138 3002 374 146 1280
Fragments arranged by size:
3002 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2292 2302 3086 3170 4067 4664
Size: 393 468 192 10 784 84 897 597
Cuts at: 4664 5451 5719 1239
Size: 787 268 1524
Fragments arranged by size:
1524 897 787 784 597 468 393 268
192 84 10
EcoO109I rG'GnC_Cy
Cuts at: 2065 2065
Size: 6004
EcoRI G'AATT_C
Cuts at: 3313 3691 3313
Size: 378 5626
FspI TGC'GCA
Cuts at: 4510 4510
Size: 6004
HaeII r_GCGC'y
Cuts at: 1678 5151 1678
Size: 3473 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2174 2206 2993 3025 4645
Size: 32 135 32 602 32 787 32 1620
Cuts at: 4645 4677 4719 4751 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1620 787 602 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3348 3594 669
Size: 988 281 1410 246 3079
Fragments arranged by size:
3079 1410 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2404 748
Size: 1656 4348
HpaI GTT'AAC
Cuts at: 3594 3594
Size: 6004
KpnI G_GTAC'C
Cuts at: 2396 3329 2396
Size: 933 5071
MfeI C'AATT_G
Cuts at: 2507 3603 2507
Size: 1096 4908
MluI A'CGCG_T
Cuts at: 5880 5880
Size: 6004
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2044 3201 5118 5302 1305
Size: 739 1157 1917 184 2007
Fragments arranged by size:
2007 1917 1157 739 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4182
Fragments arranged by size:
4182 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3962 4321 4480 518
Size: 648 2796 359 159 2042
Fragments arranged by size:
2796 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2519 3420 4097 5245 5490 1606
Size: 913 901 677 1148 245 2120
Fragments arranged by size:
2120 1148 913 901 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5452
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6004
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6004
NotI GC'GGCC_GC
Cuts at: 3451 3451
Size: 6004
NspI r_CATG'y
Cuts at: 1132 1229 2917 3362 4907 1132
Size: 97 1688 445 1545 2229
Fragments arranged by size:
2229 1688 1545 445 97
PasI CC'CwG_GG
Cuts at: 1348 3262 1348
Size: 1914 4090
PciI A'CATG_T
Cuts at: 4903 4903
Size: 6004
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2282 1108
Size: 1174 4830
PmeI GTTT'AAAC
Cuts at: 3308 3308
Size: 6004
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 5586 5618 4073
Size: 32 1481 32 4459
Fragments arranged by size:
4459 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2065 2065
Size: 6004
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6004
PsiI TTA'TAA
Cuts at: 3574 3574
Size: 6004
PstI C_TGCA'G
Cuts at: 830 2478 3356 830
Size: 1648 878 3478
Fragments arranged by size:
3478 1648 878
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6004
PvuII CAG'CTG
Cuts at: 2519 2519
Size: 6004
SalI G'TCGA_C
Cuts at: 1936 3346 1936
Size: 1410 4594
SbfI CC_TGCA'GG
Cuts at: 3356 3356
Size: 6004
ScaI AGT'ACT
Cuts at: 2323 4252 5964 2323
Size: 1929 1712 2363
Fragments arranged by size:
2363 1929 1712
SfcI C'TryA_G
Cuts at: 826 1045 2474 2754 2827 3352 4487 5168 5359
Size: 219 1429 280 73 525 1135 681 191
Cuts at: 5359 826
Size: 1471
Fragments arranged by size:
1471 1429 1135 681 525 280 219 191
73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6004
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6004
SmaI CCC'GGG
Cuts at: 1872 3331 1872
Size: 1459 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2325 4108 5009 5271 820
Size: 197 926 382 1783 901 262 1553
Fragments arranged by size:
1783 1553 926 901 382 262 197
SnaBI TAC'GTA
Cuts at: 493 3795 493
Size: 3302 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6004
SphI G_CATG'C
Cuts at: 1132 3362 1132
Size: 2230 3774
SspI AAT'ATT
Cuts at: 5 52 2568 3928 5
Size: 47 2516 1360 2081
Fragments arranged by size:
2516 2081 1360 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6004
StyI C'CwwG_G
Cuts at: 513 1065 2527 3443 513
Size: 552 1462 916 3074
Fragments arranged by size:
3074 1462 916 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4031 4048 4201 4386 4831 325
Size: 1255 2451 17 153 185 445 1498
Fragments arranged by size:
2451 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2248 2321 2820
Size: 274 80 33 51 1443 271 73 499
Cuts at: 2820 3048 3143 3153 4250 5962 96
Size: 228 95 10 1097 1712 138
Fragments arranged by size:
1712 1443 1097 499 274 271 228 138
95 80 73 51 33 10
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2113 2145 5964 5996 2113
Size: 32 3819 32 2121
Fragments arranged by size:
3819 2121 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6004
XbaI T'CTAG_A
Cuts at: 3340 3340
Size: 6004
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6004
XmaI C'CCGG_G
Cuts at: 1870 3329 1870
Size: 1459 4545
XmnI GAAnn'nnTTC
Cuts at: 4133 4133
Size: 6004
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3812 276
Size: 53 83 186 936 2278 2468
Fragments arranged by size:
2468 2278 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI